FvH4_4g23951

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011253
Physical Location & Seq
Forward (+)
26259074 .. 26262609
3536 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g23951.t1

Sequence Viewer

Length: 1113 bp
ATGTGTTTAACCACTTTGCTCAAGAAAGATACACCAATTCACTCAAAGAGCTTTCGTAATTTGAGAACCGAAGCCACAGATGATTTCGACAGCCCCATTCTACCTGGACTGCCAGATGACATATCGAAATATTGCCTTGCGCTTGTTCCTCGCTCAGACTTCTGTTCTATGAGTGTTGTGTCTAAGAAATGGAGGCACTTTATGCAAAGCAAGGAATTTACAACTGTGCGACAATTAGCTGGGTTGCTTCAGGAGTGGCTCTATGTGTTAACCATGGATGCTGAAGGAAAAGAAAGCCGTTGGGAGGTTCTGGATTGCCTTGGACATCAGCACCGTATTCTTTCTCCAATGCCTGGTCCAACAAGATCCTTGTTTGGGGTGGTAGTTCTTGGTGGAAAGCTTGTCATTGTGGGTGGTTTTTCTGTGATCGATGGGATTGGTGTTGCATCACCAGAAGTCTACAACTATGATTCTTGCATCAACAGGTGGAGCAAATTAGCTGAGATGAATGTGGCTCGATATGACTTTGCATGTGCAGAACTATGTGGCCTGGTTTACGCAGTTGGAGGGTATAGTGCAGATGGCAACATCCTATCTAGTGCTGAGGTGTATAATCCTGAAACTGATACTTGGACACTGATAGAGAGTATTCGCCGCCCAAGATATGGTTGTTTTGCGTGTGGATTTGAGGGCAAGCTGTATATTATGGGCGGAAGGTCAAGCTTCACCATTGGCAATTCAAAGTTTGTTGATGTCTACGATCCTAAGACCCACACTTGGTGTCAGATGAAGAATGGCTGTGTTATGGTCACTACACATGCTGTGCTGGAAAAGAAGCTCTTCTGTATGGAGTGGAAGAACCAGAGGAAACTTTCAATTTTCAATCCCGAGGACAATTCCTGGAAGATGGTGCAAATTCCACTGACAGGAAGCAATAGTATTGAATTTCGATTTGGGATACTGGGTGAGAAACTTCTACTGTTCTCACTACAACAGGAACTTGGTTACTGTACTTTGATGTATGATCCAAATGCAGCTCCAGGATCAGAGTGGCAGACTTCAGTGGTAAAGCTGTCTGGTCCGTGCTTGTGCTCTGTTAGAATCAATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

371

Amino Acids

41.48

Weight (kDa)

6.96

Isoelectric Point (pI)

45.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 35 - 73 7.3e-08 F-box domain
Kelch_FKB95 PF25210 120 - 214 1.6e-07 FKB95, Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 121 - 274 1.7e-08 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 122 - 170 7.5e-09 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 127 - 306 3.3e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 130 - 264 1.9e-12 Calicin, beta-propeller domain
NANM PF24996 141 - 303 4.4e-06 N-acetylneuraminate epimerase
Kelch_1 PF01344 172 - 214 1.5e-12 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 185 - 287 2.4e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 216 - 304 2.1e-09 FKB95, Kelch-repeats domain
Kelch_1 PF01344 220 - 264 1e-08 Kelch motif
Kelch_2 PF07646 220 - 264 1.7e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67480 AT1G67480 AT1G67480
fragaria_vesca FvH4_4g23951 FvH4_4g23951
malus_domestica MD13G1117000.v1.1 MD16G1117600.v1.1
prunus_persica Prupe.1G228600_v2.0.a1
pyrus_communis pycom13g10150
rosa_chinensis RchiOBHm_Chr4g0430611
rosa_laevigata RLG00000006963
rosa_multiflora Rmu_co8108234.1_g000001
rosa_roxburghii Rroxscaffold_5G00372360
rosa_rugosa Rorug04G0244800 Rorug04G0244800
rosa_samantha Rh4AG300700 Rh4BG307800 Rh4CG324900 Rh4DG305400
rosa_wichuraiana Rw4G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 353, 665
AccI GTMKAC 2 cut(s) 459, 756
AciI CCGC 2 cut(s) 655, 711
AclWI GGATC 4 cut(s) 360, 755, 1019, 1051
AcsI RAATTY 3 cut(s) 215, 915, 944
AcuI CTGAAG 3 cut(s) 233, 303, 1044
AdeI CACNNNGTG 1 cut(s) 780
AfaI GTAC 1 cut(s) 1012
AfiI CCNNNNNNNGG 6 cut(s) 304, 353, 375, 665, 777, 926
AgsI TTSAA 4 cut(s) 741, 876, 883, 944
AjnI CCWGG 5 cut(s) 103, 352, 549, 899, 1039
AjuI GAANNNNNNNTTGG 2 cut(s) 936, 968
AluBI AGCT 9 cut(s) 51, 239, 400, 500, 697, 723, 838, 1037, 1072
AluI AGCT 9 cut(s) 51, 239, 400, 500, 697, 723, 838, 1037, 1072
Alw21I GWGCWC 1 cut(s) 1094
AlwI GGATC 4 cut(s) 360, 755, 1019, 1051
Ama87I CYCGRG 1 cut(s) 887
AoxI GGCC 1 cut(s) 547
ApeKI GCWGC 1 cut(s) 1034
ApoI RAATTY 3 cut(s) 215, 915, 944
Asp700I GAANNNNTTC 1 cut(s) 839
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 2 cut(s) 356, 1079
AsuHPI GGTGA 3 cut(s) 441, 718, 977
AvaI CYCGRG 1 cut(s) 887
AvaII GGWCC 2 cut(s) 356, 1079
Bbv12I GWGCWC 1 cut(s) 1094
BbvCI CCTCAGC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 1046
BccI CCATC 3 cut(s) 425, 575, 901
BceAI ACGGC 1 cut(s) 282
BciT130I CCWGG 5 cut(s) 105, 354, 551, 901, 1041
BciVI GTATCC 1 cut(s) 951
BfaI CTAG 1 cut(s) 597
BfuI GTATCC 1 cut(s) 951
BisI GCNGC 2 cut(s) 655, 1035
BlsI GCNGC 2 cut(s) 656, 1036
Bme1390I CCNGG 5 cut(s) 105, 354, 551, 901, 1041
Bme18I GGWCC 2 cut(s) 356, 1079
BmeT110I CYCGRG 1 cut(s) 887
BmgT120I GGNCC 2 cut(s) 356, 1079
BmrFI CCNGG 5 cut(s) 105, 354, 551, 901, 1041
BmrI ACTGGG 1 cut(s) 971
BmsI GCATC 3 cut(s) 268, 455, 486
BmuI ACTGGG 1 cut(s) 971
BpmI CTGGAG 1 cut(s) 1023
Bpu10I CCTNAGC 1 cut(s) 603
BpuEI CTTGAG 1 cut(s) 5
Bsa29I ATCGAT 1 cut(s) 429
BsaJI CCNNGG 3 cut(s) 273, 319, 888
Bsc4I CCNNNNNNNGG 6 cut(s) 304, 353, 375, 665, 777, 926
Bse1I ACTGG 1 cut(s) 966
BseBI CCWGG 5 cut(s) 105, 354, 551, 901, 1041
BseCI ATCGAT 1 cut(s) 429
BseDI CCNNGG 3 cut(s) 273, 319, 888
BseGI GGATG 2 cut(s) 283, 588
BseLI CCNNNNNNNGG 6 cut(s) 304, 353, 375, 665, 777, 926
BseMII CTCAG 3 cut(s) 168, 492, 594
BseNI ACTGG 1 cut(s) 966
BseXI GCAGC 1 cut(s) 1046
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 555, 597
BshFI GGCC 1 cut(s) 549
BshVI ATCGAT 1 cut(s) 429
BsiHKAI GWGCWC 1 cut(s) 1094
BsiHKCI CYCGRG 1 cut(s) 887
BslI CCNNNNNNNGG 6 cut(s) 304, 353, 375, 665, 777, 926
BsnI GGCC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 887
Bsp1286I GDGCHC 1 cut(s) 1094
Bsp143I GATC 5 cut(s) 365, 426, 760, 1024, 1043
Bsp19I CCATGG 1 cut(s) 273
BspACI CCGC 2 cut(s) 655, 711
BspANI GGCC 1 cut(s) 549
BspCNI CTCAG 3 cut(s) 167, 493, 595
BspDI ATCGAT 1 cut(s) 429
BspPI GGATC 4 cut(s) 360, 755, 1019, 1051
BspQI GCTCTTC 1 cut(s) 845
BsrI ACTGG 1 cut(s) 966
BssECI CCNNGG 3 cut(s) 273, 319, 888
BssMI GATC 5 cut(s) 365, 426, 760, 1024, 1043
BssT1I CCWWGG 2 cut(s) 273, 319
Bst2UI CCWGG 5 cut(s) 105, 354, 551, 901, 1041
Bst4CI ACNGT 4 cut(s) 226, 335, 981, 1010
Bst6I CTCTTC 1 cut(s) 845
BstAPI GCANNNNNTGC 1 cut(s) 202
BstC8I GCNNGC 1 cut(s) 695
BstDEI CTNAG 5 cut(s) 154, 183, 501, 603, 765
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 2 cut(s) 283, 588
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 5 cut(s) 368, 429, 763, 1027, 1046
BstMBI GATC 5 cut(s) 365, 426, 760, 1024, 1043
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNI CCWGG 5 cut(s) 105, 354, 551, 901, 1041
BstNSI RCATGY 2 cut(s) 534, 821
BstSCI CCNGG 5 cut(s) 103, 352, 549, 899, 1039
BstV1I GCAGC 1 cut(s) 1046
BstX2I RGATCY 1 cut(s) 365
BstYI RGATCY 1 cut(s) 365
Bsu15I ATCGAT 1 cut(s) 429
BsuI GTATCC 1 cut(s) 951
BsuRI GGCC 1 cut(s) 549
BsuTUI ATCGAT 1 cut(s) 429
BtgI CCRYGG 1 cut(s) 273
BtsCI GGATG 2 cut(s) 283, 588
BtsIMutI CAGTG 3 cut(s) 635, 920, 1068
Cac8I GCNNGC 1 cut(s) 695
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 2 cut(s) 356, 1079
ClaI ATCGAT 1 cut(s) 429
Csp6I GTAC 1 cut(s) 1011
CviAII CATG 3 cut(s) 274, 531, 818
CviQI GTAC 1 cut(s) 1011
DdeI CTNAG 5 cut(s) 154, 183, 501, 603, 765
DpnI GATC 5 cut(s) 367, 428, 762, 1026, 1045
DpnII GATC 5 cut(s) 365, 426, 760, 1024, 1043
DraIII CACNNNGTG 1 cut(s) 780
Eam1104I CTCTTC 1 cut(s) 845
EarI CTCTTC 1 cut(s) 845
EciI GGCGGA 1 cut(s) 726
Eco130I CCWWGG 2 cut(s) 273, 319
Eco47I GGWCC 2 cut(s) 356, 1079
Eco57I CTGAAG 3 cut(s) 233, 303, 1044
Eco88I CYCGRG 1 cut(s) 887
EcoRII CCWGG 5 cut(s) 103, 352, 549, 899, 1039
EcoT14I CCWWGG 2 cut(s) 273, 319
ErhI CCWWGG 2 cut(s) 273, 319
FaeI CATG 3 cut(s) 277, 534, 821
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FatI CATG 3 cut(s) 273, 530, 817
FblI GTMKAC 2 cut(s) 459, 756
Fnu4HI GCNGC 2 cut(s) 655, 1035
FokI GGATG 2 cut(s) 290, 575
Fsp4HI GCNGC 2 cut(s) 655, 1035
FspBI CTAG 1 cut(s) 597
GlaI GCGC 1 cut(s) 141
GluI GCNGC 2 cut(s) 655, 1035
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 1023
HaeIII GGCC 1 cut(s) 549
HhaI GCGC 1 cut(s) 142
Hin1II CATG 3 cut(s) 277, 534, 821
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 270
HindII GTYRAC 1 cut(s) 270
HindIII AAGCTT 2 cut(s) 398, 721
HinfI GANTC 2 cut(s) 470, 1101
HpaI GTTAAC 1 cut(s) 270
HphI GGTGA 3 cut(s) 441, 718, 977
Hpy166II GTNNAC 4 cut(s) 270, 460, 556, 757
Hpy188I TCNGA 3 cut(s) 157, 786, 1048
Hpy188III TCNNGA 5 cut(s) 22, 251, 311, 617, 887
Hpy8I GTNNAC 4 cut(s) 270, 460, 556, 757
HpyAV CCTTC 2 cut(s) 278, 708
HpyCH4III ACNGT 4 cut(s) 226, 335, 981, 1010
HpyCH4V TGCA 8 cut(s) 205, 446, 477, 530, 536, 578, 913, 1034
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 5 cut(s) 154, 183, 501, 603, 765
Hsp92II CATG 3 cut(s) 277, 534, 821
HspAI GCGC 1 cut(s) 140
KspAI GTTAAC 1 cut(s) 270
Kzo9I GATC 5 cut(s) 365, 426, 760, 1024, 1043
LguI GCTCTTC 1 cut(s) 845
LmnI GCTCC 2 cut(s) 489, 1042
Lsp1109I GCAGC 1 cut(s) 1046
LweI GCATC 3 cut(s) 268, 455, 486
MaeI CTAG 1 cut(s) 597
MaeIII GTNAC 2 cut(s) 808, 1004
MalI GATC 5 cut(s) 367, 428, 762, 1026, 1045
MboI GATC 5 cut(s) 365, 426, 760, 1024, 1043
MboII GAAGA 4 cut(s) 802, 832, 868, 916
MflI RGATCY 1 cut(s) 365
MhlI GDGCHC 1 cut(s) 1094
MmeI TCCRAC 2 cut(s) 383, 544
MnlI CCTC 8 cut(s) 159, 186, 298, 560, 598, 682, 858, 883
MroXI GAANNNNTTC 1 cut(s) 839
MseI TTAA 2 cut(s) 8, 269
MspR9I CCNGG 5 cut(s) 105, 354, 551, 901, 1041
MvaI CCWGG 5 cut(s) 105, 354, 551, 901, 1041
MwoI GCNNNNNNNGC 1 cut(s) 202
NcoI CCATGG 1 cut(s) 273
NdeII GATC 5 cut(s) 365, 426, 760, 1024, 1043
NlaIII CATG 3 cut(s) 277, 534, 821
NmuCI GTSAC 1 cut(s) 808
NspI RCATGY 2 cut(s) 534, 821
PciSI GCTCTTC 1 cut(s) 845
PdmI GAANNNNTTC 1 cut(s) 839
PfeI GAWTC 2 cut(s) 470, 1101
PflMI CCANNNNNTGG 2 cut(s) 353, 665
PfoI TCCNGGA 2 cut(s) 899, 1039
PkrI GCNGC 2 cut(s) 656, 1036
Psp6I CCWGG 5 cut(s) 103, 352, 549, 899, 1039
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 5 cut(s) 103, 352, 549, 899, 1039
PspPI GGNCC 2 cut(s) 356, 1079
PsuI RGATCY 1 cut(s) 365
RsaI GTAC 1 cut(s) 1012
RsaNI GTAC 1 cut(s) 1011
SapI GCTCTTC 1 cut(s) 845
SaqAI TTAA 2 cut(s) 8, 269
SatI GCNGC 2 cut(s) 655, 1035
Sau3AI GATC 5 cut(s) 365, 426, 760, 1024, 1043
Sau96I GGNCC 2 cut(s) 356, 1079
ScrFI CCNGG 5 cut(s) 105, 354, 551, 901, 1041
SduI GDGCHC 1 cut(s) 1094
SfaNI GCATC 3 cut(s) 268, 455, 486
SinI GGWCC 2 cut(s) 356, 1079
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
SsiI CCGC 2 cut(s) 655, 711
SspI AATATT 1 cut(s) 131
SspMI CTAG 1 cut(s) 597
StyD4I CCNGG 5 cut(s) 103, 352, 549, 899, 1039
StyI CCWWGG 2 cut(s) 273, 319
TaaI ACNGT 4 cut(s) 226, 335, 981, 1010
TaqI TCGA 5 cut(s) 87, 125, 429, 517, 949
TatI WGTACW 1 cut(s) 1010
TauI GCSGC 1 cut(s) 657
TfiI GAWTC 2 cut(s) 470, 1101
Tru1I TTAA 2 cut(s) 8, 269
Tru9I TTAA 2 cut(s) 8, 269
TscAI CASTG 3 cut(s) 642, 927, 1068
TseFI GTSAC 1 cut(s) 808
TseI GCWGC 1 cut(s) 1034
Tsp45I GTSAC 1 cut(s) 808
TspDTI ATGAA 2 cut(s) 521, 803
TspGWI ACGGA 1 cut(s) 1071
TspRI CASTG 3 cut(s) 642, 927, 1068
Van91I CCANNNNNTGG 2 cut(s) 353, 665
VpaK11BI GGWCC 2 cut(s) 356, 1079
XapI RAATTY 3 cut(s) 215, 915, 944
XceI RCATGY 2 cut(s) 534, 821
XcmI CCANNNNNNNNNTGG 1 cut(s) 1047
XmiI GTMKAC 2 cut(s) 459, 756
XmnI GAANNNNTTC 1 cut(s) 839
XspI CTAG 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.