Rh4DG305400

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011253
Physical Location & Seq
Forward (+)
52743116 .. 52744978
1863 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG305400.1

Sequence Viewer

Length: 1158 bp
ATGCCTGATTTTGTGAGTGGAAACAAGAGATTCAAAGAACCAAATATGTGTTTAACCACTCTGCTCAAGCTAGATACACCAATCCATTCAAAAAGCTATCGTAATTTGAGTTCTGAAGCCACAGATGATTTTGACACCCCCATTCTACCTGGACTGCCAGATGACATATCGAAATGTTGCCTTGCCCTTGTTCCTCGTTCAGACTTCCATTCTATGAGTTTTGTGTGTAAGAAATGGAGGCATTTTATTCAAAGCAAGGAATTTACAACTATCCGACAATTAGCTGGGTTGCTTCAGGAGTGGCTCTATGTGTTAACCACGGATTCTGAAGGAAAGGAAAGCCATTGGGAGGTTTTGGATTGCCTTGGACATATGCACCGCATTCTTCCTCCAATGCCTGGTCCAACAAGATCTGGGTTTGGGGTGGTAGTTCTCAGCGGAAAGCTTGTTATTGTAGGTGGTTTTTCTGTGATCAATGGGACTGCTGTGGCATCATCAGAAGTCTACCATTATGATTCTTGTATCAACAGGTGGAGCAAATTAGCAGAGATGAATGTGGCTCGATATGACTTTGCTTGTGCAGAACTATCTGGCCTGGTTTACGCAGTTGGAGGCTATAGTACAGATGGCAACATCCTATCTAGTGCTGAGGTGTACAATCCTGAGACTGATACTTGGACCTTGATAGAGAGTATTCGCCGGCCAAGATATGGTTGTTTTGCATGTGGATTTGAGGGAAAGCTGTATGTTATGGGTGGAAGGTCAAGCTTCACTATTGGCAATTCAAAGTTTGTTGATGTCTACGATCCCAAGACCCACACTTGGTGTCAGATGAAGAATGGTTGTGTTATGGTCACTACTCAGGCTGTGCTGGAAAAGAAGCTCTTCTGTATGGAGTGGAAGAACCAGCGGAAACTTTCAATTTTCAATCCTGAGGACAATTCCTGGAAGATGGTGCAAATTCCACTGACAGGAAGCACTAGTATTGAATTTCGATTTGGGATACTGGGTGAGAAACTTCTACTGTTCTCACTAGAGCAGGAACCTGGTTACCGTACTCTGACGTATGATCCAAATGCAGCTCCAGGATCAGAGTGGCAGACTTCAGAGGTAGAGCTGTCTGGTCCGTGCTTGTGCACTGTTAGAATCACGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

385

Amino Acids

43.2

Weight (kDa)

6.1

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 50 - 89 7.5e-09 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 115 - 279 6.9e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 133 - 268 1.4e-07 FBX42, beta-propeller domain
Kelch_1 PF01344 137 - 185 2.4e-09 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 141 - 321 2.2e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 141 - 280 5.5e-13 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 163 - 230 5.5e-08 FKB95, Kelch-repeats domain
Kelch_1 PF01344 187 - 229 7.8e-12 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 200 - 302 4.3e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 226 - 299 2.6e-08 FKB95, Kelch-repeats domain
Kelch_1 PF01344 235 - 279 5.1e-09 Kelch motif
Kelch_2 PF07646 235 - 279 1.5e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67480 AT1G67480 AT1G67480
fragaria_vesca FvH4_4g23951 FvH4_4g23951
malus_domestica MD13G1117000.v1.1 MD16G1117600.v1.1
prunus_persica Prupe.1G228600_v2.0.a1
pyrus_communis pycom13g10150
rosa_chinensis RchiOBHm_Chr4g0430611
rosa_laevigata RLG00000006963
rosa_multiflora Rmu_co8108234.1_g000001
rosa_roxburghii Rroxscaffold_5G00372360
rosa_rugosa Rorug04G0244800 Rorug04G0244800
rosa_samantha Rh4AG300700 Rh4BG307800 Rh4CG324900 Rh4DG305400
rosa_wichuraiana Rw4G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 398, 710
AccI GTMKAC 2 cut(s) 504, 801
AciI CCGC 3 cut(s) 379, 438, 910
AclWI GGATC 3 cut(s) 800, 1064, 1096
AcoI YGGCCR 1 cut(s) 701
AcsI RAATTY 3 cut(s) 260, 960, 989
AcuI CTGAAG 4 cut(s) 135, 278, 348, 1089
AdeI CACNNNGTG 2 cut(s) 825, 1155
AfaI GTAC 3 cut(s) 622, 656, 1057
AfiI CCNNNNNNNGG 5 cut(s) 349, 398, 710, 822, 971
AgsI TTSAA 7 cut(s) 34, 90, 251, 786, 921, 928, 989
AhlI ACTAGT 1 cut(s) 980
AjnI CCWGG 6 cut(s) 148, 397, 594, 944, 1045, 1084
AjuI GAANNNNNNNTTGG 2 cut(s) 981, 1013
AluBI AGCT 9 cut(s) 70, 96, 284, 445, 742, 768, 883, 1082, 1117
AluI AGCT 9 cut(s) 70, 96, 284, 445, 742, 768, 883, 1082, 1117
Alw21I GWGCWC 1 cut(s) 1139
Alw26I GTCTC 1 cut(s) 659
Alw44I GTGCAC 1 cut(s) 1135
AlwI GGATC 3 cut(s) 800, 1064, 1096
AoxI GGCC 2 cut(s) 592, 701
ApaLI GTGCAC 1 cut(s) 1135
ApeKI GCWGC 1 cut(s) 1079
ApoI RAATTY 3 cut(s) 260, 960, 989
Asp700I GAANNNNTTC 1 cut(s) 884
AspS9I GGNCC 3 cut(s) 401, 678, 1124
AsuHPI GGTGA 1 cut(s) 1022
AvaII GGWCC 3 cut(s) 401, 678, 1124
AxyI CCTNAGG 1 cut(s) 933
BaeGI GKGCMC 1 cut(s) 1139
Bbv12I GWGCWC 1 cut(s) 1139
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 1 cut(s) 1091
BccI CCATC 2 cut(s) 620, 946
BciT130I CCWGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
BciVI GTATCC 1 cut(s) 996
BclI TGATCA 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 659
BcuI ACTAGT 1 cut(s) 980
BfaI CTAG 4 cut(s) 71, 642, 981, 1034
BfmI CTRYAG 1 cut(s) 616
BfuI GTATCC 1 cut(s) 996
BglII AGATCT 1 cut(s) 410
BisI GCNGC 1 cut(s) 1080
BlsI GCNGC 1 cut(s) 1081
Bme1390I CCNGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
Bme18I GGWCC 3 cut(s) 401, 678, 1124
BmgT120I GGNCC 3 cut(s) 401, 678, 1124
BmiI GGNNCC 1 cut(s) 1044
BmrFI CCNGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
BmrI ACTGGG 1 cut(s) 1016
BmsI GCATC 1 cut(s) 500
BmuI ACTGGG 1 cut(s) 1016
BpmI CTGGAG 1 cut(s) 1068
Bpu10I CCTNAGC 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 50
BsaJI CCNNGG 2 cut(s) 318, 364
Bsc4I CCNNNNNNNGG 5 cut(s) 349, 398, 710, 822, 971
Bse118I RCCGGY 1 cut(s) 699
Bse1I ACTGG 1 cut(s) 1011
Bse21I CCTNAGG 1 cut(s) 933
BseBI CCWGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
BseDI CCNNGG 2 cut(s) 318, 364
BseGI GGATG 1 cut(s) 633
BseLI CCNNNNNNNGG 5 cut(s) 349, 398, 710, 822, 971
BseMII CTCAG 5 cut(s) 448, 639, 654, 875, 924
BseNI ACTGG 1 cut(s) 1011
BseSI GKGCMC 1 cut(s) 1139
BseXI GCAGC 1 cut(s) 1091
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 600
BshFI GGCC 2 cut(s) 594, 703
BsiHKAI GWGCWC 1 cut(s) 1139
BsiSI CCGG 1 cut(s) 700
BslFI GGGAC 1 cut(s) 493
BslI CCNNNNNNNGG 5 cut(s) 349, 398, 710, 822, 971
BsmAI GTCTC 1 cut(s) 659
BsmFI GGGAC 1 cut(s) 493
BsmI GAATGC 1 cut(s) 381
BsnI GGCC 2 cut(s) 594, 703
Bsp1286I GDGCHC 1 cut(s) 1139
Bsp1407I TGTACA 1 cut(s) 654
Bsp143I GATC 5 cut(s) 410, 471, 805, 1069, 1088
BspACI CCGC 3 cut(s) 379, 438, 910
BspANI GGCC 2 cut(s) 594, 703
BspCNI CTCAG 5 cut(s) 447, 640, 655, 874, 925
BspLI GGNNCC 1 cut(s) 1044
BspPI GGATC 3 cut(s) 800, 1064, 1096
BspQI GCTCTTC 1 cut(s) 890
BsrFI RCCGGY 1 cut(s) 699
BsrGI TGTACA 1 cut(s) 654
BsrI ACTGG 1 cut(s) 1011
BssAI RCCGGY 1 cut(s) 699
BssECI CCNNGG 2 cut(s) 318, 364
BssMI GATC 5 cut(s) 410, 471, 805, 1069, 1088
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
Bst4CI ACNGT 4 cut(s) 1026, 1055, 1141, 1153
Bst6I CTCTTC 1 cut(s) 890
BstAUI TGTACA 1 cut(s) 654
BstC8I GCNNGC 1 cut(s) 701
BstDEI CTNAG 5 cut(s) 434, 648, 663, 861, 933
BstDSI CCRYGG 1 cut(s) 318
BstEII GGTNACC 1 cut(s) 1049
BstF5I GGATG 1 cut(s) 633
BstKTI GATC 5 cut(s) 413, 474, 808, 1072, 1091
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 5 cut(s) 410, 471, 805, 1069, 1088
BstNI CCWGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
BstNSI RCATGY 1 cut(s) 726
BstPI GGTNACC 1 cut(s) 1049
BstSCI CCNGG 6 cut(s) 148, 397, 594, 944, 1045, 1084
BstSFI CTRYAG 1 cut(s) 616
BstSLI GKGCMC 1 cut(s) 1139
BstV1I GCAGC 1 cut(s) 1091
BstX2I RGATCY 1 cut(s) 410
BstYI RGATCY 1 cut(s) 410
Bsu36I CCTNAGG 1 cut(s) 933
BsuI GTATCC 1 cut(s) 996
BsuRI GGCC 2 cut(s) 594, 703
BtgI CCRYGG 1 cut(s) 318
BtsCI GGATG 1 cut(s) 633
BtsIMutI CAGTG 2 cut(s) 965, 1137
Cac8I GCNNGC 1 cut(s) 701
Cfr10I RCCGGY 1 cut(s) 699
Cfr13I GGNCC 3 cut(s) 401, 678, 1124
CsiI ACCWGGT 1 cut(s) 1045
Csp6I GTAC 3 cut(s) 621, 655, 1056
CviAII CATG 1 cut(s) 723
CviQI GTAC 3 cut(s) 621, 655, 1056
DdeI CTNAG 5 cut(s) 434, 648, 663, 861, 933
DpnI GATC 5 cut(s) 412, 473, 807, 1071, 1090
DpnII GATC 5 cut(s) 410, 471, 805, 1069, 1088
DraIII CACNNNGTG 2 cut(s) 825, 1155
EaeI YGGCCR 1 cut(s) 701
Eam1104I CTCTTC 1 cut(s) 890
EarI CTCTTC 1 cut(s) 890
Eco130I CCWWGG 1 cut(s) 364
Eco47I GGWCC 3 cut(s) 401, 678, 1124
Eco57I CTGAAG 4 cut(s) 135, 278, 348, 1089
Eco81I CCTNAGG 1 cut(s) 933
Eco91I GGTNACC 1 cut(s) 1049
EcoO65I GGTNACC 1 cut(s) 1049
EcoRII CCWGG 6 cut(s) 148, 397, 594, 944, 1045, 1084
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 1 cut(s) 726
FalI AAGNNNNNCTT 2 cut(s) 869, 901
FaqI GGGAC 1 cut(s) 493
FatI CATG 1 cut(s) 722
FauNDI CATATG 1 cut(s) 372
FbaI TGATCA 1 cut(s) 471
FblI GTMKAC 2 cut(s) 504, 801
Fnu4HI GCNGC 1 cut(s) 1080
FokI GGATG 1 cut(s) 620
Fsp4HI GCNGC 1 cut(s) 1080
FspBI CTAG 4 cut(s) 71, 642, 981, 1034
GluI GCNGC 1 cut(s) 1080
GsaI CCCAGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 1068
HaeIII GGCC 2 cut(s) 594, 703
HapII CCGG 1 cut(s) 700
Hin1II CATG 1 cut(s) 726
HincII GTYRAC 1 cut(s) 315
HindII GTYRAC 1 cut(s) 315
HindIII AAGCTT 2 cut(s) 443, 766
HinfI GANTC 4 cut(s) 30, 323, 515, 1146
HpaI GTTAAC 1 cut(s) 315
HpaII CCGG 1 cut(s) 700
HphI GGTGA 1 cut(s) 1022
Hpy166II GTNNAC 6 cut(s) 315, 505, 601, 655, 802, 1137
Hpy188I TCNGA 9 cut(s) 115, 202, 275, 328, 499, 831, 1062, 1093, 1108
Hpy188III TCNNGA 3 cut(s) 296, 662, 932
Hpy8I GTNNAC 6 cut(s) 315, 505, 601, 655, 802, 1137
HpyAV CCTTC 2 cut(s) 323, 753
HpyCH4III ACNGT 4 cut(s) 1026, 1055, 1141, 1153
HpyCH4IV ACGT 1 cut(s) 1064
HpyCH4V TGCA 6 cut(s) 376, 581, 722, 958, 1079, 1137
HpyF3I CTNAG 5 cut(s) 434, 648, 663, 861, 933
HpySE526I ACGT 1 cut(s) 1064
Hsp92II CATG 1 cut(s) 726
KroI GCCGGC 1 cut(s) 699
KroNI GCCGGC 1 cut(s) 701
Ksp22I TGATCA 1 cut(s) 471
KspAI GTTAAC 1 cut(s) 315
Kzo9I GATC 5 cut(s) 410, 471, 805, 1069, 1088
LguI GCTCTTC 1 cut(s) 890
LmnI GCTCC 2 cut(s) 534, 1087
Lsp1109I GCAGC 1 cut(s) 1091
LweI GCATC 1 cut(s) 500
MabI ACCWGGT 1 cut(s) 1045
MaeI CTAG 4 cut(s) 71, 642, 981, 1034
MaeII ACGT 1 cut(s) 1064
MaeIII GTNAC 2 cut(s) 853, 1049
MalI GATC 5 cut(s) 412, 473, 807, 1071, 1090
MboI GATC 5 cut(s) 410, 471, 805, 1069, 1088
MboII GAAGA 5 cut(s) 377, 847, 877, 913, 961
MflI RGATCY 1 cut(s) 410
MhlI GDGCHC 1 cut(s) 1139
MluCI AATT 9 cut(s) 103, 260, 278, 539, 781, 921, 940, 960, 989
MmeI TCCRAC 3 cut(s) 298, 428, 589
MnlI CCTC 9 cut(s) 204, 231, 343, 399, 605, 643, 727, 928, 1102
MroNI GCCGGC 1 cut(s) 699
MroXI GAANNNNTTC 1 cut(s) 884
MseI TTAA 2 cut(s) 53, 314
MspA1I CMGCKG 2 cut(s) 438, 910
MspI CCGG 1 cut(s) 700
MspR9I CCNGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
Mva1269I GAATGC 1 cut(s) 381
MvaI CCWGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
NaeI GCCGGC 1 cut(s) 701
NdeI CATATG 1 cut(s) 372
NdeII GATC 5 cut(s) 410, 471, 805, 1069, 1088
NgoMIV GCCGGC 1 cut(s) 699
NlaIII CATG 1 cut(s) 726
NlaIV GGNNCC 1 cut(s) 1044
NmuCI GTSAC 1 cut(s) 853
NspI RCATGY 1 cut(s) 726
PciSI GCTCTTC 1 cut(s) 890
PctI GAATGC 1 cut(s) 381
PdiI GCCGGC 1 cut(s) 701
PdmI GAANNNNTTC 1 cut(s) 884
PfeI GAWTC 4 cut(s) 30, 323, 515, 1146
PflMI CCANNNNNTGG 2 cut(s) 398, 710
PfoI TCCNGGA 2 cut(s) 944, 1084
PkrI GCNGC 1 cut(s) 1081
Psp6I CCWGG 6 cut(s) 148, 397, 594, 944, 1045, 1084
PspEI GGTNACC 1 cut(s) 1049
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 6 cut(s) 148, 397, 594, 944, 1045, 1084
PspN4I GGNNCC 1 cut(s) 1044
PspPI GGNCC 3 cut(s) 401, 678, 1124
PsrI GAACNNNNNNTAC 2 cut(s) 94, 126
PsuI RGATCY 1 cut(s) 410
RsaI GTAC 3 cut(s) 622, 656, 1057
RsaNI GTAC 3 cut(s) 621, 655, 1056
SapI GCTCTTC 1 cut(s) 890
SaqAI TTAA 2 cut(s) 53, 314
SatI GCNGC 1 cut(s) 1080
Sau3AI GATC 5 cut(s) 410, 471, 805, 1069, 1088
Sau96I GGNCC 3 cut(s) 401, 678, 1124
ScrFI CCNGG 6 cut(s) 150, 399, 596, 946, 1047, 1086
SduI GDGCHC 1 cut(s) 1139
SexAI ACCWGGT 1 cut(s) 1045
SfaNI GCATC 1 cut(s) 500
SfcI CTRYAG 1 cut(s) 616
SinI GGWCC 3 cut(s) 401, 678, 1124
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
SpeI ACTAGT 1 cut(s) 980
Sse9I AATT 9 cut(s) 103, 260, 278, 539, 781, 921, 940, 960, 989
SsiI CCGC 3 cut(s) 379, 438, 910
SspMI CTAG 4 cut(s) 71, 642, 981, 1034
StyD4I CCNGG 6 cut(s) 148, 397, 594, 944, 1045, 1084
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 4 cut(s) 1026, 1055, 1141, 1153
TaiI ACGT 1 cut(s) 1067
TaqI TCGA 3 cut(s) 170, 562, 994
TasI AATT 9 cut(s) 103, 260, 278, 539, 781, 921, 940, 960, 989
TatI WGTACW 2 cut(s) 620, 654
TfiI GAWTC 4 cut(s) 30, 323, 515, 1146
Tru1I TTAA 2 cut(s) 53, 314
Tru9I TTAA 2 cut(s) 53, 314
TscAI CASTG 2 cut(s) 972, 1144
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 1 cut(s) 1079
Tsp45I GTSAC 1 cut(s) 853
TspDTI ATGAA 2 cut(s) 566, 848
TspGWI ACGGA 2 cut(s) 335, 1116
TspRI CASTG 2 cut(s) 972, 1144
Van91I CCANNNNNTGG 2 cut(s) 398, 710
VneI GTGCAC 1 cut(s) 1135
VpaK11BI GGWCC 3 cut(s) 401, 678, 1124
XapI RAATTY 3 cut(s) 260, 960, 989
XceI RCATGY 1 cut(s) 726
XcmI CCANNNNNNNNNTGG 1 cut(s) 1092
XmiI GTMKAC 2 cut(s) 504, 801
XmnI GAANNNNTTC 1 cut(s) 884
XspI CTAG 4 cut(s) 71, 642, 981, 1034
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.