MD13G1117000.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011253
Physical Location & Seq
Reverse (-)
8545775 .. 8549280
3506 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1117000.v1.1.491

Sequence Viewer

Length: 1116 bp
ATGTGCCGATCGAATTTGGTCAAGCAAGATGCACCAATTGGTGCAAAAAGCTATCGCAGTTTGACTTCTCTGGAGGTAGATGATTCTGAGAGCCCCATCCTGCCGGGACTGCCAGATGACGTAGCAAAATATTGTCTAGCGCTTGTTCCTCATTCAGACTTCCCTGCTATGGGTGGCGTGTGTAAGAAATGGAGGTATTTTCTTCAAAGCAAAGAACTTATCACTGTGCGGCAATTAGCTGGGTTGCTTGAGGAGTGGCTCTATGTTTTGACAACCGATTCCGAAGGAAAGGAAAACCACTGGGAGGTTTTAGATTGCCATGGACATAAGCACCATGTTCTTCCTCCCATGCCTGGTCCAACAAGATTTGGATTTGGGGTGGCGGTTCTTAATGGAAAGCTTCTTGTCGTTGGTGGATATTCTGCGATTCCCGGGACCACCGTTGCATCAGAAGATGTGTACCAATACGATTCTTGCCTCAACAGGTGGGGCAAATTGGCAAACATGAATGTTGCTCGACATGACTTTGCTTGTGCAGAACTAAGTGGTATGATTTACGCTGTTGGGGGATATGGTATAGATGGCAGCAGTCTATCCAGTGCCGAGGTGTACAATCCTGACACCGATACTTGGACCCTGATAAGCAGTATTCGCCGTCCTCGATATGGTTGCTTTGCCTGTGGATTTGAAGGAAAGCTGTATGTTATGGGCGGAAGGTCAAGCTTCAGTATTGGAAATTCAAAGTTTGTTGATGTCTACGACCCTGAGAGTCACACTTGGGGTGAGATGAAGAAGAATGGTTGTGTCATGGTCACTGCTCATGCTGTGCTGGAAAAGAAGCTGTTCTGTTTGGAGTGGAGGAACCAGCGGAAACTTTCAATTTACAACCCTGACGACAATTCTTGGGAGATGGTGCAGATTCCGTTGACAGGAAGCACAAGCATCAAGCTCCGATTTGGGATACTGGATGACAAACTTCTGTTGTTTTCACTCGAGGAGGATCCTGGTTATCGTACTTTGTTGTACGATCCAAATGCAGCACCAGGATCCGAGTGGCTGACTTCTGAGGTAAAGCTGTCCGGTCCCTGCTTGTGCTGTGTTACCATCAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

372

Amino Acids

41.11

Weight (kDa)

5.76

Isoelectric Point (pI)

33.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kelch_KLHDC2_KLHL20_DRC7 PF24681 87 - 216 7.5e-08 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 113 - 260 9.1e-12 Calicin, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 118 - 277 1.5e-09 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 122 - 170 9.2e-09 Kelch motif
Beta-prop_FBX42 PF13415 122 - 268 3.6e-06 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 127 - 308 2.9e-14 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_FKB95 PF25210 149 - 214 1.9e-07 FKB95, Kelch-repeats domain
NANM PF24996 162 - 339 1.9e-06 N-acetylneuraminate epimerase
Kelch_1 PF01344 172 - 216 3.5e-10 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 185 - 276 4e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 210 - 307 1.7e-11 FKB95, Kelch-repeats domain
Kelch_1 PF01344 220 - 260 3.4e-07 Kelch motif
Kelch_2 PF07646 220 - 263 7.6e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67480 AT1G67480 AT1G67480
fragaria_vesca FvH4_4g23951 FvH4_4g23951
malus_domestica MD13G1117000.v1.1 MD16G1117600.v1.1
prunus_persica Prupe.1G228600_v2.0.a1
pyrus_communis pycom13g10150
rosa_chinensis RchiOBHm_Chr4g0430611
rosa_laevigata RLG00000006963
rosa_multiflora Rmu_co8108234.1_g000001
rosa_roxburghii Rroxscaffold_5G00372360
rosa_rugosa Rorug04G0244800 Rorug04G0244800
rosa_samantha Rh4AG300700 Rh4BG307800 Rh4CG324900 Rh4DG305400
rosa_wichuraiana Rw4G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 756
AciI CCGC 4 cut(s) 229, 383, 711, 868
AclWI GGATC 5 cut(s) 995, 1008, 1022, 1041, 1054
AcsI RAATTY 2 cut(s) 13, 736
AcuI CTGAAG 1 cut(s) 709
AfaI GTAC 4 cut(s) 461, 611, 1015, 1025
AfeI AGCGCT 1 cut(s) 141
AfiI CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
AgsI TTSAA 4 cut(s) 206, 689, 741, 879
AjnI CCWGG 3 cut(s) 352, 1003, 1042
AluBI AGCT 8 cut(s) 51, 239, 400, 697, 723, 841, 949, 1075
AluI AGCT 8 cut(s) 51, 239, 400, 697, 723, 841, 949, 1075
AlwI GGATC 5 cut(s) 995, 1008, 1022, 1041, 1054
Ama87I CYCGRG 2 cut(s) 431, 992
Aor51HI AGCGCT 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 585, 1037
ApoI RAATTY 2 cut(s) 13, 736
Asp700I GAANNNNTTC 1 cut(s) 842
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 4 cut(s) 356, 435, 633, 1082
AsuC2I CCSGG 3 cut(s) 105, 432, 433
AsuHPI GGTGA 1 cut(s) 794
AvaI CYCGRG 2 cut(s) 431, 992
AvaII GGWCC 4 cut(s) 356, 435, 633, 1082
BamHI GGATCC 2 cut(s) 1000, 1046
BanII GRGCYC 1 cut(s) 95
BbvI GCAGC 2 cut(s) 597, 1049
BccI CCATC 4 cut(s) 104, 575, 904, 1112
BceAI ACGGC 1 cut(s) 639
BcgI CGANNNNNNTGC 4 cut(s) 651, 685, 1016, 1050
BciT130I CCWGG 3 cut(s) 354, 1005, 1044
BciVI GTATCC 1 cut(s) 954
BcnI CCSGG 3 cut(s) 105, 432, 433
BfaI CTAG 1 cut(s) 137
BfoI RGCGCY 1 cut(s) 143
BfuI GTATCC 1 cut(s) 954
BisI GCNGC 3 cut(s) 230, 586, 1038
BlsI GCNGC 3 cut(s) 231, 587, 1039
Bme1390I CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
Bme18I GGWCC 4 cut(s) 356, 435, 633, 1082
BmeT110I CYCGRG 2 cut(s) 431, 992
BmgT120I GGNCC 4 cut(s) 356, 435, 633, 1082
BmiI GGNNCC 6 cut(s) 436, 635, 863, 1002, 1048, 1084
BmrFI CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 3 cut(s) 19, 455, 951
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 92
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 3 cut(s) 105, 432, 433
BsaJI CCNNGG 3 cut(s) 319, 431, 603
BsaWI WCCGGW 1 cut(s) 1079
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
Bse1I ACTGG 3 cut(s) 305, 597, 969
BseBI CCWGG 3 cut(s) 354, 1005, 1044
BseDI CCNNGG 3 cut(s) 319, 431, 603
BseGI GGATG 2 cut(s) 96, 973
BseLI CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
BseMII CTCAG 3 cut(s) 78, 756, 1056
BseNI ACTGG 3 cut(s) 305, 597, 969
BseRI GAGGAG 2 cut(s) 266, 1010
BseXI GCAGC 2 cut(s) 597, 1049
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 555, 935
Bsh1285I CGRYCG 1 cut(s) 11
BsiEI CGRYCG 1 cut(s) 11
BsiHKCI CYCGRG 2 cut(s) 431, 992
BsiSI CCGG 3 cut(s) 104, 432, 1080
BslFI GGGAC 3 cut(s) 120, 448, 1068
BslI CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
BsmFI GGGAC 3 cut(s) 120, 448, 1068
BsoBI CYCGRG 2 cut(s) 431, 992
Bsp1286I GDGCHC 1 cut(s) 95
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 4 cut(s) 8, 1000, 1027, 1046
Bsp19I CCATGG 1 cut(s) 319
BspACI CCGC 4 cut(s) 229, 383, 711, 868
BspCNI CTCAG 3 cut(s) 79, 757, 1057
BspLI GGNNCC 6 cut(s) 436, 635, 863, 1002, 1048, 1084
BspPI GGATC 5 cut(s) 995, 1008, 1022, 1041, 1054
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 3 cut(s) 305, 597, 969
BssECI CCNNGG 3 cut(s) 319, 431, 603
BssMI GATC 4 cut(s) 8, 1000, 1027, 1046
BssT1I CCWWGG 1 cut(s) 319
Bst2UI CCWGG 3 cut(s) 354, 1005, 1044
Bst4CI ACNGT 2 cut(s) 226, 442
BstAUI TGTACA 1 cut(s) 609
BstDEI CTNAG 4 cut(s) 87, 542, 765, 1065
BstDSI CCRYGG 1 cut(s) 319
BstF5I GGATG 2 cut(s) 96, 973
BstH2I RGCGCY 1 cut(s) 143
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 4 cut(s) 11, 1003, 1030, 1049
BstMBI GATC 4 cut(s) 8, 1000, 1027, 1046
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 2 cut(s) 109, 651
BstNI CCWGG 3 cut(s) 354, 1005, 1044
BstSCI CCNGG 6 cut(s) 103, 352, 430, 431, 1003, 1042
BstV1I GCAGC 2 cut(s) 597, 1049
BstX2I RGATCY 2 cut(s) 1000, 1046
BstYI RGATCY 2 cut(s) 1000, 1046
BsuI GTATCC 1 cut(s) 954
BtgI CCRYGG 1 cut(s) 319
BtsCI GGATG 2 cut(s) 96, 973
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 4 cut(s) 222, 298, 604, 813
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 4 cut(s) 356, 435, 633, 1082
Cfr9I CCCGGG 1 cut(s) 431
Csp6I GTAC 4 cut(s) 460, 610, 1014, 1024
CviAII CATG 7 cut(s) 320, 335, 349, 505, 521, 808, 821
CviQI GTAC 4 cut(s) 460, 610, 1014, 1024
DdeI CTNAG 4 cut(s) 87, 542, 765, 1065
DpnI GATC 4 cut(s) 10, 1002, 1029, 1048
DpnII GATC 4 cut(s) 8, 1000, 1027, 1046
EciI GGCGGA 1 cut(s) 726
Eco130I CCWWGG 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 95
Eco47I GGWCC 4 cut(s) 356, 435, 633, 1082
Eco47III AGCGCT 1 cut(s) 141
Eco57I CTGAAG 1 cut(s) 709
Eco88I CYCGRG 2 cut(s) 431, 992
EcoRII CCWGG 3 cut(s) 352, 1003, 1042
EcoT14I CCWWGG 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 95
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
FaqI GGGAC 3 cut(s) 120, 448, 1068
FatI CATG 7 cut(s) 319, 334, 348, 504, 520, 807, 820
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 3 cut(s) 230, 586, 1038
FokI GGATG 2 cut(s) 83, 980
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 3 cut(s) 230, 586, 1038
FspBI CTAG 1 cut(s) 137
GlaI GCGC 1 cut(s) 141
GluI GCNGC 3 cut(s) 230, 586, 1038
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 143
HapII CCGG 3 cut(s) 104, 432, 1080
HhaI GCGC 1 cut(s) 142
Hin1II CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HindIII AAGCTT 2 cut(s) 398, 721
HinfI GANTC 6 cut(s) 83, 278, 427, 470, 769, 919
HpaII CCGG 3 cut(s) 104, 432, 1080
HphI GGTGA 1 cut(s) 794
Hpy166II GTNNAC 4 cut(s) 460, 610, 757, 927
Hpy188I TCNGA 7 cut(s) 88, 157, 283, 451, 953, 1051, 1066
Hpy188III TCNNGA 2 cut(s) 71, 617
Hpy8I GTNNAC 4 cut(s) 460, 610, 757, 927
HpyAV CCTTC 3 cut(s) 278, 683, 708
HpyCH4III ACNGT 2 cut(s) 226, 442
HpyCH4IV ACGT 1 cut(s) 120
HpyCH4V TGCA 6 cut(s) 32, 44, 446, 536, 916, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 651
HpyF3I CTNAG 4 cut(s) 87, 542, 765, 1065
HpySE526I ACGT 1 cut(s) 120
Hsp92II CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 4 cut(s) 8, 1000, 1027, 1046
LmnI GCTCC 1 cut(s) 954
Lsp1109I GCAGC 2 cut(s) 597, 1049
LweI GCATC 3 cut(s) 19, 455, 951
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 120
MaeIII GTNAC 3 cut(s) 770, 811, 1099
MalI GATC 4 cut(s) 10, 1002, 1029, 1048
MboI GATC 4 cut(s) 8, 1000, 1027, 1046
MboII GAAGA 5 cut(s) 194, 332, 464, 802, 805
MfeI CAATTG 1 cut(s) 36
MflI RGATCY 2 cut(s) 1000, 1046
MhlI GDGCHC 1 cut(s) 95
MluCI AATT 7 cut(s) 13, 36, 233, 494, 736, 879, 898
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 1 cut(s) 383
MroXI GAANNNNTTC 1 cut(s) 842
MseI TTAA 1 cut(s) 390
MslI CAYNNNNRTG 1 cut(s) 1109
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 3 cut(s) 104, 432, 1080
MspR9I CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
MunI CAATTG 1 cut(s) 36
MvaI CCWGG 3 cut(s) 354, 1005, 1044
MwoI GCNNNNNNNGC 2 cut(s) 109, 651
NciI CCSGG 3 cut(s) 105, 432, 433
NcoI CCATGG 1 cut(s) 319
NdeII GATC 4 cut(s) 8, 1000, 1027, 1046
NlaIII CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
NlaIV GGNNCC 6 cut(s) 436, 635, 863, 1002, 1048, 1084
NmeAIII GCCGAG 1 cut(s) 628
NmuCI GTSAC 2 cut(s) 770, 811
PaeR7I CTCGAG 1 cut(s) 992
PcsI WCGNNNNNNNCGW 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 842
PfeI GAWTC 5 cut(s) 83, 278, 427, 470, 919
PkrI GCNGC 3 cut(s) 231, 587, 1039
Ple19I CGATCG 1 cut(s) 11
PleI GAGTC 1 cut(s) 777
PpsI GAGTC 1 cut(s) 777
Psp6I CCWGG 3 cut(s) 352, 1003, 1042
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 3 cut(s) 352, 1003, 1042
PspN4I GGNNCC 6 cut(s) 436, 635, 863, 1002, 1048, 1084
PspPI GGNCC 4 cut(s) 356, 435, 633, 1082
PspXI VCTCGAGB 1 cut(s) 992
PsuI RGATCY 2 cut(s) 1000, 1046
PvuI CGATCG 1 cut(s) 11
RsaI GTAC 4 cut(s) 461, 611, 1015, 1025
RsaNI GTAC 4 cut(s) 460, 610, 1014, 1024
RseI CAYNNNNRTG 1 cut(s) 1109
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 3 cut(s) 230, 586, 1038
Sau3AI GATC 4 cut(s) 8, 1000, 1027, 1046
Sau96I GGNCC 4 cut(s) 356, 435, 633, 1082
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
SduI GDGCHC 1 cut(s) 95
SfaNI GCATC 3 cut(s) 19, 455, 951
Sfr274I CTCGAG 1 cut(s) 992
SinI GGWCC 4 cut(s) 356, 435, 633, 1082
SlaI CTCGAG 1 cut(s) 992
SmaI CCCGGG 1 cut(s) 433
SmiMI CAYNNNNRTG 1 cut(s) 1109
SmlI CTYRAG 2 cut(s) 248, 992
SmoI CTYRAG 2 cut(s) 248, 992
Sse9I AATT 7 cut(s) 13, 36, 233, 494, 736, 879, 898
SsiI CCGC 4 cut(s) 229, 383, 711, 868
SspI AATATT 1 cut(s) 131
SspMI CTAG 1 cut(s) 137
StyD4I CCNGG 6 cut(s) 103, 352, 430, 431, 1003, 1042
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 226, 442
TaiI ACGT 1 cut(s) 123
TaqI TCGA 4 cut(s) 11, 517, 661, 993
TasI AATT 7 cut(s) 13, 36, 233, 494, 736, 879, 898
TatI WGTACW 1 cut(s) 609
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 5 cut(s) 83, 278, 427, 470, 919
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 4 cut(s) 229, 305, 604, 820
TseFI GTSAC 2 cut(s) 770, 811
TseI GCWGC 2 cut(s) 585, 1037
Tsp45I GTSAC 2 cut(s) 770, 811
TspDTI ATGAA 2 cut(s) 521, 803
TspGWI ACGGA 1 cut(s) 912
TspMI CCCGGG 1 cut(s) 431
TspRI CASTG 4 cut(s) 229, 305, 604, 820
VpaK11BI GGWCC 4 cut(s) 356, 435, 633, 1082
XapI RAATTY 2 cut(s) 13, 736
XcmI CCANNNNNNNNNTGG 1 cut(s) 1050
XhoI CTCGAG 1 cut(s) 992
XmaI CCCGGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 1 cut(s) 842
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.