Rw4G026090

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011253
Physical Location & Seq
Forward (+)
52302263 .. 52305681
3419 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G026090.1

Sequence Viewer

Length: 1239 bp
ATGGAGGTGATTATATATCATGGAAGTTACAGGGGGCTAAAACCTGCCAAGTATATGGCATTGACTGACACTCGGGTCATGTGTGGAGGAACAAACAACTCAGAAAGCAACTACTCTTGGAGCAGAATTTTTGTTATGGAATTAGGACCTGACTGGACAAAACATAGAAAATTGCCAGCATATACTGCTCATGTGATGCGTTCTGCAACATTTTTTATGCCTTGGTCTTGTAAAATTATAGATGACATATCGAAATGTTGCCTTGCCCTTGTTCCTCGTTCAGATTTCCATTCTATGAGTTTTGTGTGTAAGAAATGGAGGCATTTTATTCAAAGCAAGGAATTTACAACTATCCGACAATTAGCTGGGTTGCTTCAGGAGTGGCTCTATGTGTTAACCACGGATTCTGAAGGAAAGGAAAGCCATTGGGAGGTTTTGGATTGCCTTGGACATATGCACCGCATTCTTCCTCCAATGCCTGGTCCAACAAGATCTGGGTTTGGGGTGGTAGTTCTCAGCGGAAAGCTTGTTATTGTAGGTGGTTTTTCTGTGATCAATGGGACTGCTGTGGCCTCATCAGAAGTCTACCAGTATGATTCTTGTATCAACAGGTGGAGCAAATTAGCAGAGATGAATGTGGCTCGATATGACTTTGCTTGTGCAGAACTATCTGGCCTGGTTTACGCAGTTGGAGGCTATAGTACAGATGGCAACATCCTATCTAGTGCTGAGGTGTACAATCCTGAGACTGATACTTGGACCTTGATAGAGAGTATTCGCCGGCCAAGATATGGTTGTTTTGCATGTGGATTTGAGGGAAAGCTGTATGTTATGGGTGGAAGGTCAAGCTTCACTATTGGCAATTCAAAGTTTGTTGATGTCTACGATCCCAAGACCCACACTTGGTGTCAGATGAAGAATGGTTGTGTTATGGTCACTACTCAGGCTGTGCTGGAAAAGAAGCTCTTCTGTATGGAGTGGAAGAACCAGCGGAAACTTTCAATTTTCAATCCTGAGGACAATTCCTGGAAGATGGTGCAAATTCCACTGACAGGAAGCACTAGTATTGAATTTCGATTTGGGATACTGGGTGAGAAACTTCTACTGTTCTCACTAGAGCAGGAACCTGGTTACCGTACTCTGACGTATGATCCAAATGCAGCTCCAGGATCAGAGTGGCAGACTTCAGAGGTAGAGCTGTCTGGTCCGTGCTTGTGCACTGTTAGAATCACGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

412

Amino Acids

46.52

Weight (kDa)

7.85

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 81 - 116 6.8e-06 F-box domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 142 - 306 9.7e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_FBX42 PF13415 160 - 295 2.1e-07 FBX42, beta-propeller domain
Beta-prop_ATRN-LZTR1 PF24981 162 - 307 2e-09 Attractin/LZTR1 beta-propeller
Kelch_1 PF01344 164 - 212 1.1e-09 Kelch motif
Kelch_KLHDC2_KLHL20_DRC7 PF24681 168 - 348 3.4e-13 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_Calicin PF13964 168 - 307 5.7e-13 Calicin, beta-propeller domain
Kelch_FKB95 PF25210 190 - 257 7.9e-08 FKB95, Kelch-repeats domain
Kelch_1 PF01344 214 - 256 8.6e-12 Kelch motif
Beta-prop_ATRN-LZTR1 PF24981 227 - 329 4.9e-06 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 252 - 326 6.5e-09 FKB95, Kelch-repeats domain
Kelch_1 PF01344 262 - 306 5.6e-09 Kelch motif
Kelch_2 PF07646 262 - 306 1.7e-07 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67480 AT1G67480 AT1G67480
fragaria_vesca FvH4_4g23951 FvH4_4g23951
malus_domestica MD13G1117000.v1.1 MD16G1117600.v1.1
prunus_persica Prupe.1G228600_v2.0.a1
pyrus_communis pycom13g10150
rosa_chinensis RchiOBHm_Chr4g0430611
rosa_laevigata RLG00000006963
rosa_multiflora Rmu_co8108234.1_g000001
rosa_roxburghii Rroxscaffold_5G00372360
rosa_rugosa Rorug04G0244800 Rorug04G0244800
rosa_samantha Rh4AG300700 Rh4BG307800 Rh4CG324900 Rh4DG305400
rosa_wichuraiana Rw4G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 52
AccB7I CCANNNNNTGG 2 cut(s) 479, 791
AccI GTMKAC 2 cut(s) 585, 882
AciI CCGC 3 cut(s) 460, 519, 991
AclWI GGATC 3 cut(s) 881, 1145, 1177
AcoI YGGCCR 1 cut(s) 782
AcsI RAATTY 4 cut(s) 126, 341, 1041, 1070
AcuI CTGAAG 3 cut(s) 359, 429, 1170
AdeI CACNNNGTG 2 cut(s) 906, 1236
AfaI GTAC 3 cut(s) 703, 737, 1138
AfiI CCNNNNNNNGG 5 cut(s) 430, 479, 791, 903, 1052
AgsI TTSAA 5 cut(s) 332, 867, 1002, 1009, 1070
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 5 cut(s) 478, 675, 1025, 1126, 1165
AjuI GAANNNNNNNTTGG 2 cut(s) 1062, 1094
AluBI AGCT 7 cut(s) 365, 526, 823, 849, 964, 1163, 1198
AluI AGCT 7 cut(s) 365, 526, 823, 849, 964, 1163, 1198
Alw21I GWGCWC 1 cut(s) 1220
Alw26I GTCTC 1 cut(s) 740
Alw44I GTGCAC 1 cut(s) 1216
AlwI GGATC 3 cut(s) 881, 1145, 1177
Ama87I CYCGRG 1 cut(s) 72
AoxI GGCC 3 cut(s) 570, 673, 782
ApaLI GTGCAC 1 cut(s) 1216
ApeKI GCWGC 1 cut(s) 1160
ApoI RAATTY 4 cut(s) 126, 341, 1041, 1070
Asp700I GAANNNNTTC 1 cut(s) 965
AspS9I GGNCC 4 cut(s) 146, 482, 759, 1205
AsuHPI GGTGA 2 cut(s) 19, 1103
AvaI CYCGRG 1 cut(s) 72
AvaII GGWCC 4 cut(s) 146, 482, 759, 1205
AxyI CCTNAGG 1 cut(s) 1014
BaeGI GKGCMC 1 cut(s) 1220
Bbv12I GWGCWC 1 cut(s) 1220
BbvCI CCTCAGC 1 cut(s) 729
BbvI GCAGC 1 cut(s) 1172
BccI CCATC 2 cut(s) 701, 1027
BciT130I CCWGG 5 cut(s) 480, 677, 1027, 1128, 1167
BciVI GTATCC 1 cut(s) 1077
BclI TGATCA 1 cut(s) 552
BcoDI GTCTC 1 cut(s) 740
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 3 cut(s) 723, 1062, 1115
BfmI CTRYAG 1 cut(s) 697
BfuAI ACCTGC 1 cut(s) 52
BfuI GTATCC 1 cut(s) 1077
BglII AGATCT 1 cut(s) 491
BisI GCNGC 1 cut(s) 1161
BlsI GCNGC 1 cut(s) 1162
Bme1390I CCNGG 5 cut(s) 480, 677, 1027, 1128, 1167
Bme18I GGWCC 4 cut(s) 146, 482, 759, 1205
BmeT110I CYCGRG 1 cut(s) 72
BmgT120I GGNCC 4 cut(s) 146, 482, 759, 1205
BmiI GGNNCC 1 cut(s) 1125
BmrFI CCNGG 5 cut(s) 480, 677, 1027, 1128, 1167
BmrI ACTGGG 1 cut(s) 1097
BmsI GCATC 1 cut(s) 186
BmuI ACTGGG 1 cut(s) 1097
BpmI CTGGAG 1 cut(s) 1149
Bpu10I CCTNAGC 1 cut(s) 729
BsaJI CCNNGG 3 cut(s) 221, 399, 445
Bsc4I CCNNNNNNNGG 5 cut(s) 430, 479, 791, 903, 1052
Bse118I RCCGGY 1 cut(s) 780
Bse1I ACTGG 3 cut(s) 158, 589, 1092
Bse21I CCTNAGG 1 cut(s) 1014
BseBI CCWGG 5 cut(s) 480, 677, 1027, 1128, 1167
BseDI CCNNGG 3 cut(s) 221, 399, 445
BseGI GGATG 1 cut(s) 714
BseLI CCNNNNNNNGG 5 cut(s) 430, 479, 791, 903, 1052
BseMII CTCAG 6 cut(s) 114, 529, 720, 735, 956, 1005
BseNI ACTGG 3 cut(s) 158, 589, 1092
BseSI GKGCMC 1 cut(s) 1220
BseXI GCAGC 1 cut(s) 1172
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 681
BshFI GGCC 3 cut(s) 572, 675, 784
BsiHKAI GWGCWC 1 cut(s) 1220
BsiHKCI CYCGRG 1 cut(s) 72
BsiSI CCGG 1 cut(s) 781
BslFI GGGAC 1 cut(s) 574
BslI CCNNNNNNNGG 5 cut(s) 430, 479, 791, 903, 1052
BsmAI GTCTC 1 cut(s) 740
BsmFI GGGAC 1 cut(s) 574
BsmI GAATGC 1 cut(s) 462
BsnI GGCC 3 cut(s) 572, 675, 784
BsoBI CYCGRG 1 cut(s) 72
Bsp1286I GDGCHC 1 cut(s) 1220
Bsp1407I TGTACA 1 cut(s) 735
Bsp143I GATC 5 cut(s) 491, 552, 886, 1150, 1169
BspACI CCGC 3 cut(s) 460, 519, 991
BspANI GGCC 3 cut(s) 572, 675, 784
BspCNI CTCAG 6 cut(s) 113, 528, 721, 736, 955, 1006
BspLI GGNNCC 1 cut(s) 1125
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 3 cut(s) 881, 1145, 1177
BspQI GCTCTTC 1 cut(s) 971
BsrFI RCCGGY 1 cut(s) 780
BsrGI TGTACA 1 cut(s) 735
BsrI ACTGG 3 cut(s) 158, 589, 1092
BssAI RCCGGY 1 cut(s) 780
BssECI CCNNGG 3 cut(s) 221, 399, 445
BssMI GATC 5 cut(s) 491, 552, 886, 1150, 1169
BssT1I CCWWGG 2 cut(s) 221, 445
Bst2UI CCWGG 5 cut(s) 480, 677, 1027, 1128, 1167
Bst4CI ACNGT 4 cut(s) 1107, 1136, 1222, 1234
Bst6I CTCTTC 1 cut(s) 971
BstAPI GCANNNNNTGC 1 cut(s) 185
BstAUI TGTACA 1 cut(s) 735
BstC8I GCNNGC 2 cut(s) 177, 782
BstDEI CTNAG 6 cut(s) 100, 515, 729, 744, 942, 1014
BstDSI CCRYGG 1 cut(s) 399
BstEII GGTNACC 1 cut(s) 1130
BstF5I GGATG 1 cut(s) 714
BstKTI GATC 5 cut(s) 494, 555, 889, 1153, 1172
BstMAI GTCTC 1 cut(s) 740
BstMBI GATC 5 cut(s) 491, 552, 886, 1150, 1169
BstMWI GCNNNNNNNGC 1 cut(s) 185
BstNI CCWGG 5 cut(s) 480, 677, 1027, 1128, 1167
BstNSI RCATGY 1 cut(s) 807
BstPI GGTNACC 1 cut(s) 1130
BstSCI CCNGG 5 cut(s) 478, 675, 1025, 1126, 1165
BstSFI CTRYAG 1 cut(s) 697
BstSLI GKGCMC 1 cut(s) 1220
BstV1I GCAGC 1 cut(s) 1172
BstX2I RGATCY 1 cut(s) 491
BstXI CCANNNNNNTGG 1 cut(s) 55
BstYI RGATCY 1 cut(s) 491
Bsu36I CCTNAGG 1 cut(s) 1014
BsuI GTATCC 1 cut(s) 1077
BsuRI GGCC 3 cut(s) 572, 675, 784
BtgI CCRYGG 1 cut(s) 399
BtsCI GGATG 1 cut(s) 714
BtsIMutI CAGTG 2 cut(s) 1046, 1218
BveI ACCTGC 1 cut(s) 52
Cac8I GCNNGC 2 cut(s) 177, 782
Cfr10I RCCGGY 1 cut(s) 780
Cfr13I GGNCC 4 cut(s) 146, 482, 759, 1205
CsiI ACCWGGT 1 cut(s) 1126
Csp6I GTAC 3 cut(s) 702, 736, 1137
CviAII CATG 4 cut(s) 20, 79, 191, 804
CviQI GTAC 3 cut(s) 702, 736, 1137
DdeI CTNAG 6 cut(s) 100, 515, 729, 744, 942, 1014
DpnI GATC 5 cut(s) 493, 554, 888, 1152, 1171
DpnII GATC 5 cut(s) 491, 552, 886, 1150, 1169
DraIII CACNNNGTG 2 cut(s) 906, 1236
DrdI GACNNNNNNGTC 1 cut(s) 74
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 1 cut(s) 782
Eam1104I CTCTTC 1 cut(s) 971
EarI CTCTTC 1 cut(s) 971
Eco130I CCWWGG 2 cut(s) 221, 445
Eco47I GGWCC 4 cut(s) 146, 482, 759, 1205
Eco57I CTGAAG 3 cut(s) 359, 429, 1170
Eco81I CCTNAGG 1 cut(s) 1014
Eco88I CYCGRG 1 cut(s) 72
Eco91I GGTNACC 1 cut(s) 1130
EcoO109I RGGNCCY 1 cut(s) 146
EcoO65I GGTNACC 1 cut(s) 1130
EcoRII CCWGG 5 cut(s) 478, 675, 1025, 1126, 1165
EcoT14I CCWWGG 2 cut(s) 221, 445
ErhI CCWWGG 2 cut(s) 221, 445
FaeI CATG 4 cut(s) 23, 82, 194, 807
FalI AAGNNNNNCTT 2 cut(s) 950, 982
FaqI GGGAC 1 cut(s) 574
FatI CATG 4 cut(s) 19, 78, 190, 803
FauNDI CATATG 1 cut(s) 453
FbaI TGATCA 1 cut(s) 552
FblI GTMKAC 2 cut(s) 585, 882
Fnu4HI GCNGC 1 cut(s) 1161
FokI GGATG 1 cut(s) 701
Fsp4HI GCNGC 1 cut(s) 1161
FspBI CTAG 3 cut(s) 723, 1062, 1115
GluI GCNGC 1 cut(s) 1161
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 1149
HaeIII GGCC 3 cut(s) 572, 675, 784
HapII CCGG 1 cut(s) 781
Hin1II CATG 4 cut(s) 23, 82, 194, 807
HincII GTYRAC 1 cut(s) 396
HindII GTYRAC 1 cut(s) 396
HindIII AAGCTT 2 cut(s) 524, 847
HinfI GANTC 3 cut(s) 404, 596, 1227
HpaI GTTAAC 1 cut(s) 396
HpaII CCGG 1 cut(s) 781
HphI GGTGA 2 cut(s) 19, 1103
Hpy166II GTNNAC 6 cut(s) 396, 586, 682, 736, 883, 1218
Hpy188I TCNGA 9 cut(s) 103, 283, 356, 409, 580, 912, 1143, 1174, 1189
Hpy188III TCNNGA 3 cut(s) 377, 743, 1013
Hpy8I GTNNAC 6 cut(s) 396, 586, 682, 736, 883, 1218
HpyAV CCTTC 2 cut(s) 404, 834
HpyCH4III ACNGT 4 cut(s) 1107, 1136, 1222, 1234
HpyCH4IV ACGT 1 cut(s) 1145
HpyCH4V TGCA 7 cut(s) 206, 457, 662, 803, 1039, 1160, 1218
HpyF10VI GCNNNNNNNGC 1 cut(s) 185
HpyF3I CTNAG 6 cut(s) 100, 515, 729, 744, 942, 1014
HpySE526I ACGT 1 cut(s) 1145
Hsp92II CATG 4 cut(s) 23, 82, 194, 807
KroI GCCGGC 1 cut(s) 780
KroNI GCCGGC 1 cut(s) 782
Ksp22I TGATCA 1 cut(s) 552
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 5 cut(s) 491, 552, 886, 1150, 1169
LguI GCTCTTC 1 cut(s) 971
LmnI GCTCC 3 cut(s) 120, 615, 1168
Lsp1109I GCAGC 1 cut(s) 1172
LweI GCATC 1 cut(s) 186
MabI ACCWGGT 1 cut(s) 1126
MaeI CTAG 3 cut(s) 723, 1062, 1115
MaeII ACGT 1 cut(s) 1145
MaeIII GTNAC 3 cut(s) 26, 934, 1130
MalI GATC 5 cut(s) 493, 554, 888, 1152, 1171
MboI GATC 5 cut(s) 491, 552, 886, 1150, 1169
MboII GAAGA 5 cut(s) 458, 928, 958, 994, 1042
MflI RGATCY 1 cut(s) 491
MhlI GDGCHC 1 cut(s) 1220
MmeI TCCRAC 3 cut(s) 379, 509, 670
MroNI GCCGGC 1 cut(s) 780
MroXI GAANNNNTTC 1 cut(s) 965
MseI TTAA 1 cut(s) 395
MspA1I CMGCKG 2 cut(s) 519, 991
MspI CCGG 1 cut(s) 781
MspR9I CCNGG 5 cut(s) 480, 677, 1027, 1128, 1167
Mva1269I GAATGC 1 cut(s) 462
MvaI CCWGG 5 cut(s) 480, 677, 1027, 1128, 1167
MwoI GCNNNNNNNGC 1 cut(s) 185
NaeI GCCGGC 1 cut(s) 782
NdeI CATATG 1 cut(s) 453
NdeII GATC 5 cut(s) 491, 552, 886, 1150, 1169
NgoMIV GCCGGC 1 cut(s) 780
NlaIII CATG 4 cut(s) 23, 82, 194, 807
NlaIV GGNNCC 1 cut(s) 1125
NmuCI GTSAC 1 cut(s) 934
NspI RCATGY 1 cut(s) 807
PciSI GCTCTTC 1 cut(s) 971
PctI GAATGC 1 cut(s) 462
PdiI GCCGGC 1 cut(s) 782
PdmI GAANNNNTTC 1 cut(s) 965
PfeI GAWTC 3 cut(s) 404, 596, 1227
PflMI CCANNNNNTGG 2 cut(s) 479, 791
PfoI TCCNGGA 2 cut(s) 1025, 1165
PkrI GCNGC 1 cut(s) 1162
PpuMI RGGWCCY 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 5 cut(s) 478, 675, 1025, 1126, 1165
PspEI GGTNACC 1 cut(s) 1130
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 5 cut(s) 478, 675, 1025, 1126, 1165
PspN4I GGNNCC 1 cut(s) 1125
PspPI GGNCC 4 cut(s) 146, 482, 759, 1205
PspPPI RGGWCCY 1 cut(s) 146
PsuI RGATCY 1 cut(s) 491
RsaI GTAC 3 cut(s) 703, 737, 1138
RsaNI GTAC 3 cut(s) 702, 736, 1137
SapI GCTCTTC 1 cut(s) 971
SaqAI TTAA 1 cut(s) 395
SatI GCNGC 1 cut(s) 1161
Sau3AI GATC 5 cut(s) 491, 552, 886, 1150, 1169
Sau96I GGNCC 4 cut(s) 146, 482, 759, 1205
ScrFI CCNGG 5 cut(s) 480, 677, 1027, 1128, 1167
SduI GDGCHC 1 cut(s) 1220
SexAI ACCWGGT 1 cut(s) 1126
SfaNI GCATC 1 cut(s) 186
SfcI CTRYAG 1 cut(s) 697
SinI GGWCC 4 cut(s) 146, 482, 759, 1205
SpeI ACTAGT 1 cut(s) 1061
SsiI CCGC 3 cut(s) 460, 519, 991
SspMI CTAG 3 cut(s) 723, 1062, 1115
StyD4I CCNGG 5 cut(s) 478, 675, 1025, 1126, 1165
StyI CCWWGG 2 cut(s) 221, 445
TaaI ACNGT 4 cut(s) 1107, 1136, 1222, 1234
TaiI ACGT 1 cut(s) 1148
TaqI TCGA 3 cut(s) 251, 643, 1075
TatI WGTACW 2 cut(s) 701, 735
TfiI GAWTC 3 cut(s) 404, 596, 1227
Tru1I TTAA 1 cut(s) 395
Tru9I TTAA 1 cut(s) 395
TscAI CASTG 2 cut(s) 1053, 1225
TseFI GTSAC 1 cut(s) 934
TseI GCWGC 1 cut(s) 1160
Tsp45I GTSAC 1 cut(s) 934
TspDTI ATGAA 2 cut(s) 647, 929
TspGWI ACGGA 2 cut(s) 416, 1197
TspRI CASTG 2 cut(s) 1053, 1225
Van91I CCANNNNNTGG 2 cut(s) 479, 791
VneI GTGCAC 1 cut(s) 1216
VpaK11BI GGWCC 4 cut(s) 146, 482, 759, 1205
XapI RAATTY 4 cut(s) 126, 341, 1041, 1070
XceI RCATGY 1 cut(s) 807
XcmI CCANNNNNNNNNTGG 1 cut(s) 1173
XmiI GTMKAC 2 cut(s) 585, 882
XmnI GAANNNNTTC 1 cut(s) 965
XspI CTAG 3 cut(s) 723, 1062, 1115
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.