pycom13g10150

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011253
Physical Location & Seq
Reverse (-)
6797021 .. 6798356
1336 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g10150.2

Sequence Viewer

Length: 1116 bp
ATGTGCCGATCCAATTCGGTCAAGCAAGATGCACCAATTAGTGCAAAAAGCTATCGGAGTTTGACTTCTCTGGAGGTAGATGATTCTGACAGCCCCATCCTGCCGGGACTGCCAGATGACATAGCAAAATATTGTCTAGCGCGTGTTCCTCATTCAGACTTCCCTGCTATGGGTGGCGTGTGTAAGAAATGGAGGTATTTTCTTCAAAGCAAAGAACTTATCACTGTGCGGCAATTAGCTGGGTTGCTTGAGGAGTGGCTCTATGTTTTGACAACCGATTCTCAAGGAATGGAAAACCACTGGGAGGTTTTGGATTGCCATGGACATAAGCACCATGTTCTTCCTCCCATGCCTGGTCCAACAAGATTTGGATTTGGGGTGGCGGTTCTTAATGGAAAGCTTCTTGTCGTTGGTGGATATTCTGTGATTCCCGGGACCACCATTGCATCAGAAGATGTGTACCAATACGATTCTTGCCTCAACAGGTGGGGCAAATTGGCAAACATGAATGTTGCTCGACATGACTTTGCTTGTGCAGAACTAAGTGGTATGATTTACGCTGTTGGAGGATACGGTATAGATGGCAGCAGTCTATCCAGTGCCGAGGTGTACAATCCTGACACCGATACTTGGACCCTGATAAGCAGTATTCGCCGTCCTCGATATGGTTGCTTTGCCTGTGGATTTGAAGGAAAGCTGTATGTTATGGGCGGAAGGTCAAGCTTCACAATTGGAAATTCAAAGTTTGTTGATGTCTACGACCCTGAGAGTCACACTTGGGGTGAGATGAAGAAGAATGGTTGTGTCATGGTCACTGCTCATGCTGTGCTGGAAAAGAAGCTGTTCTGTTTGGAGTGGAAGAACCAGCGGAAACTTTCAATTTACAACCCTGACGACAATTCTTGGGAGATGGTGCAGATTCCGTTGACAGGAAGCACAAGCATCAAGCTCCGATTTGGGATACTGGATGACAAACTTCTGTTGTTTTCGCTCGAGGAGGATCCTGGTTATCGTACTTTGTTGTACGATCCAAATGCAGCACCAGGATCCGAGTGGCTGACTTCTGAGGTAAAGCTGTCCGGCCCCTGCTTGTGCTGTGTTACTATCAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

372

Amino Acids

41.18

Weight (kDa)

5.87

Isoelectric Point (pI)

33.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67480 AT1G67480 AT1G67480
fragaria_vesca FvH4_4g23951 FvH4_4g23951
malus_domestica MD13G1117000.v1.1 MD16G1117600.v1.1
prunus_persica Prupe.1G228600_v2.0.a1
pyrus_communis pycom13g10150
rosa_chinensis RchiOBHm_Chr4g0430611
rosa_laevigata RLG00000006963
rosa_multiflora Rmu_co8108234.1_g000001
rosa_roxburghii Rroxscaffold_5G00372360
rosa_rugosa Rorug04G0244800 Rorug04G0244800
rosa_samantha Rh4AG300700 Rh4BG307800 Rh4CG324900 Rh4DG305400
rosa_wichuraiana Rw4G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 756
AccII CGCG 1 cut(s) 142
AciI CCGC 4 cut(s) 229, 383, 711, 868
AclWI GGATC 6 cut(s) 3, 995, 1008, 1022, 1041, 1054
AcsI RAATTY 1 cut(s) 736
AfaI GTAC 4 cut(s) 461, 611, 1015, 1025
AfiI CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
AgsI TTSAA 4 cut(s) 206, 689, 741, 879
AjnI CCWGG 3 cut(s) 352, 1003, 1042
AluBI AGCT 8 cut(s) 51, 239, 400, 697, 723, 841, 949, 1075
AluI AGCT 8 cut(s) 51, 239, 400, 697, 723, 841, 949, 1075
AlwI GGATC 6 cut(s) 3, 995, 1008, 1022, 1041, 1054
Ama87I CYCGRG 2 cut(s) 431, 992
AoxI GGCC 1 cut(s) 1081
ApeKI GCWGC 2 cut(s) 585, 1037
ApoI RAATTY 1 cut(s) 736
Asp700I GAANNNNTTC 1 cut(s) 842
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 4 cut(s) 356, 435, 633, 1082
AsuC2I CCSGG 3 cut(s) 105, 432, 433
AsuHPI GGTGA 1 cut(s) 794
AvaI CYCGRG 2 cut(s) 431, 992
AvaII GGWCC 3 cut(s) 356, 435, 633
BamHI GGATCC 2 cut(s) 1000, 1046
BbvI GCAGC 2 cut(s) 597, 1049
BccI CCATC 3 cut(s) 104, 575, 904
BceAI ACGGC 1 cut(s) 639
BcgI CGANNNNNNTGC 4 cut(s) 651, 685, 1016, 1050
BciT130I CCWGG 3 cut(s) 354, 1005, 1044
BciVI GTATCC 2 cut(s) 563, 954
BcnI CCSGG 3 cut(s) 105, 432, 433
BfaI CTAG 1 cut(s) 137
BfuI GTATCC 2 cut(s) 563, 954
BisI GCNGC 3 cut(s) 230, 586, 1038
BlsI GCNGC 3 cut(s) 231, 587, 1039
Bme1390I CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
Bme18I GGWCC 3 cut(s) 356, 435, 633
BmeT110I CYCGRG 2 cut(s) 431, 992
BmgT120I GGNCC 4 cut(s) 356, 435, 633, 1082
BmiI GGNNCC 5 cut(s) 436, 635, 1002, 1048, 1084
BmrFI CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
BmrI ACTGGG 1 cut(s) 310
BmsI GCATC 3 cut(s) 19, 455, 951
BmuI ACTGGG 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 92
BpuEI CTTGAG 2 cut(s) 267, 269
BpuMI CCSGG 3 cut(s) 105, 432, 433
BsaJI CCNNGG 3 cut(s) 319, 431, 603
Bsc4I CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
Bse1I ACTGG 3 cut(s) 305, 597, 969
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 3 cut(s) 354, 1005, 1044
BseDI CCNNGG 3 cut(s) 319, 431, 603
BseGI GGATG 2 cut(s) 96, 973
BseLI CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 2 cut(s) 756, 1056
BseNI ACTGG 3 cut(s) 305, 597, 969
BseRI GAGGAG 2 cut(s) 266, 1010
BseXI GCAGC 2 cut(s) 597, 1049
BseYI CCCAGC 1 cut(s) 239
BsgI GTGCAG 2 cut(s) 555, 935
Bsh1236I CGCG 1 cut(s) 142
BshFI GGCC 1 cut(s) 1083
BsiHKCI CYCGRG 2 cut(s) 431, 992
BsiSI CCGG 3 cut(s) 104, 432, 1080
BslFI GGGAC 2 cut(s) 120, 448
BslI CCNNNNNNNGG 7 cut(s) 169, 170, 304, 353, 630, 665, 929
BsmFI GGGAC 2 cut(s) 120, 448
BsnI GGCC 1 cut(s) 1083
BsoBI CYCGRG 2 cut(s) 431, 992
Bsp1407I TGTACA 1 cut(s) 609
Bsp143I GATC 4 cut(s) 8, 1000, 1027, 1046
Bsp19I CCATGG 1 cut(s) 319
BspACI CCGC 4 cut(s) 229, 383, 711, 868
BspANI GGCC 1 cut(s) 1083
BspCNI CTCAG 2 cut(s) 757, 1057
BspFNI CGCG 1 cut(s) 142
BspLI GGNNCC 5 cut(s) 436, 635, 1002, 1048, 1084
BspPI GGATC 6 cut(s) 3, 995, 1008, 1022, 1041, 1054
BsrDI GCAATG 1 cut(s) 441
BsrGI TGTACA 1 cut(s) 609
BsrI ACTGG 3 cut(s) 305, 597, 969
BssECI CCNNGG 3 cut(s) 319, 431, 603
BssMI GATC 4 cut(s) 8, 1000, 1027, 1046
BssT1I CCWWGG 1 cut(s) 319
Bst2UI CCWGG 3 cut(s) 354, 1005, 1044
Bst4CI ACNGT 2 cut(s) 226, 575
BstAUI TGTACA 1 cut(s) 609
BstDEI CTNAG 3 cut(s) 542, 765, 1065
BstDSI CCRYGG 1 cut(s) 319
BstF5I GGATG 2 cut(s) 96, 973
BstFNI CGCG 1 cut(s) 142
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 4 cut(s) 11, 1003, 1030, 1049
BstMBI GATC 4 cut(s) 8, 1000, 1027, 1046
BstMWI GCNNNNNNNGC 2 cut(s) 109, 651
BstNI CCWGG 3 cut(s) 354, 1005, 1044
BstSCI CCNGG 6 cut(s) 103, 352, 430, 431, 1003, 1042
BstUI CGCG 1 cut(s) 142
BstV1I GCAGC 2 cut(s) 597, 1049
BstX2I RGATCY 2 cut(s) 1000, 1046
BstYI RGATCY 2 cut(s) 1000, 1046
BsuI GTATCC 2 cut(s) 563, 954
BsuRI GGCC 1 cut(s) 1083
BtgI CCRYGG 1 cut(s) 319
BtsCI GGATG 2 cut(s) 96, 973
BtsI GCAGTG 1 cut(s) 813
BtsIMutI CAGTG 4 cut(s) 222, 298, 604, 813
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 4 cut(s) 356, 435, 633, 1082
Cfr9I CCCGGG 1 cut(s) 431
Csp6I GTAC 4 cut(s) 460, 610, 1014, 1024
CviAII CATG 7 cut(s) 320, 335, 349, 505, 521, 808, 821
CviQI GTAC 4 cut(s) 460, 610, 1014, 1024
DdeI CTNAG 3 cut(s) 542, 765, 1065
DpnI GATC 4 cut(s) 10, 1002, 1029, 1048
DpnII GATC 4 cut(s) 8, 1000, 1027, 1046
EciI GGCGGA 1 cut(s) 726
Eco130I CCWWGG 1 cut(s) 319
Eco47I GGWCC 3 cut(s) 356, 435, 633
Eco88I CYCGRG 2 cut(s) 431, 992
EcoRII CCWGG 3 cut(s) 352, 1003, 1042
EcoT14I CCWWGG 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 319
FaeI CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
FaqI GGGAC 2 cut(s) 120, 448
FatI CATG 7 cut(s) 319, 334, 348, 504, 520, 807, 820
FblI GTMKAC 1 cut(s) 756
Fnu4HI GCNGC 3 cut(s) 230, 586, 1038
FokI GGATG 2 cut(s) 83, 980
Fsp4HI GCNGC 3 cut(s) 230, 586, 1038
FspBI CTAG 1 cut(s) 137
GlaI GCGC 1 cut(s) 141
GluI GCNGC 3 cut(s) 230, 586, 1038
GsaI CCCAGC 1 cut(s) 243
GsuI CTGGAG 1 cut(s) 92
HaeIII GGCC 1 cut(s) 1083
HapII CCGG 3 cut(s) 104, 432, 1080
HhaI GCGC 1 cut(s) 142
Hin1II CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HincII GTYRAC 1 cut(s) 927
HindII GTYRAC 1 cut(s) 927
HindIII AAGCTT 2 cut(s) 398, 721
HinfI GANTC 6 cut(s) 83, 278, 427, 470, 769, 919
HpaII CCGG 3 cut(s) 104, 432, 1080
HphI GGTGA 1 cut(s) 794
Hpy166II GTNNAC 4 cut(s) 460, 610, 757, 927
Hpy188I TCNGA 7 cut(s) 57, 88, 157, 451, 953, 1051, 1066
Hpy188III TCNNGA 2 cut(s) 71, 617
Hpy8I GTNNAC 4 cut(s) 460, 610, 757, 927
HpyAV CCTTC 2 cut(s) 683, 708
HpyCH4III ACNGT 2 cut(s) 226, 575
HpyCH4V TGCA 6 cut(s) 32, 44, 446, 536, 916, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 109, 651
HpyF3I CTNAG 3 cut(s) 542, 765, 1065
Hsp92II CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
HspAI GCGC 1 cut(s) 140
Kzo9I GATC 4 cut(s) 8, 1000, 1027, 1046
LmnI GCTCC 1 cut(s) 954
Lsp1109I GCAGC 2 cut(s) 597, 1049
LweI GCATC 3 cut(s) 19, 455, 951
MaeI CTAG 1 cut(s) 137
MaeIII GTNAC 3 cut(s) 770, 811, 1099
MalI GATC 4 cut(s) 10, 1002, 1029, 1048
MboI GATC 4 cut(s) 8, 1000, 1027, 1046
MboII GAAGA 6 cut(s) 194, 332, 464, 802, 805, 871
MfeI CAATTG 1 cut(s) 729
MflI RGATCY 2 cut(s) 1000, 1046
MluCI AATT 8 cut(s) 13, 36, 233, 494, 729, 736, 879, 898
MlyI GAGTC 1 cut(s) 778
MmeI TCCRAC 2 cut(s) 383, 544
MroXI GAANNNNTTC 1 cut(s) 842
MseI TTAA 1 cut(s) 390
MspA1I CMGCKG 1 cut(s) 868
MspI CCGG 3 cut(s) 104, 432, 1080
MspR9I CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
MunI CAATTG 1 cut(s) 729
MvaI CCWGG 3 cut(s) 354, 1005, 1044
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 109, 651
NciI CCSGG 3 cut(s) 105, 432, 433
NcoI CCATGG 1 cut(s) 319
NdeII GATC 4 cut(s) 8, 1000, 1027, 1046
NlaIII CATG 7 cut(s) 323, 338, 352, 508, 524, 811, 824
NlaIV GGNNCC 5 cut(s) 436, 635, 1002, 1048, 1084
NmeAIII GCCGAG 1 cut(s) 628
NmuCI GTSAC 2 cut(s) 770, 811
PaeR7I CTCGAG 1 cut(s) 992
PcsI WCGNNNNNNNCGW 1 cut(s) 658
PdmI GAANNNNTTC 1 cut(s) 842
PfeI GAWTC 5 cut(s) 83, 278, 427, 470, 919
PkrI GCNGC 3 cut(s) 231, 587, 1039
PleI GAGTC 1 cut(s) 777
PpsI GAGTC 1 cut(s) 777
Psp6I CCWGG 3 cut(s) 352, 1003, 1042
PspFI CCCAGC 1 cut(s) 239
PspGI CCWGG 3 cut(s) 352, 1003, 1042
PspN4I GGNNCC 5 cut(s) 436, 635, 1002, 1048, 1084
PspPI GGNCC 4 cut(s) 356, 435, 633, 1082
PspXI VCTCGAGB 1 cut(s) 992
PsuI RGATCY 2 cut(s) 1000, 1046
RsaI GTAC 4 cut(s) 461, 611, 1015, 1025
RsaNI GTAC 4 cut(s) 460, 610, 1014, 1024
SaqAI TTAA 1 cut(s) 390
SatI GCNGC 3 cut(s) 230, 586, 1038
Sau3AI GATC 4 cut(s) 8, 1000, 1027, 1046
Sau96I GGNCC 4 cut(s) 356, 435, 633, 1082
SchI GAGTC 1 cut(s) 778
ScrFI CCNGG 6 cut(s) 105, 354, 432, 433, 1005, 1044
SfaNI GCATC 3 cut(s) 19, 455, 951
Sfr274I CTCGAG 1 cut(s) 992
SinI GGWCC 3 cut(s) 356, 435, 633
SlaI CTCGAG 1 cut(s) 992
SmaI CCCGGG 1 cut(s) 433
SmlI CTYRAG 3 cut(s) 248, 282, 992
SmoI CTYRAG 3 cut(s) 248, 282, 992
Sse9I AATT 8 cut(s) 13, 36, 233, 494, 729, 736, 879, 898
SsiI CCGC 4 cut(s) 229, 383, 711, 868
SspI AATATT 1 cut(s) 131
SspMI CTAG 1 cut(s) 137
StyD4I CCNGG 6 cut(s) 103, 352, 430, 431, 1003, 1042
StyI CCWWGG 1 cut(s) 319
TaaI ACNGT 2 cut(s) 226, 575
TaqI TCGA 3 cut(s) 517, 661, 993
TaqII GACCGA 1 cut(s) 7
TasI AATT 8 cut(s) 13, 36, 233, 494, 729, 736, 879, 898
TatI WGTACW 1 cut(s) 609
TauI GCSGC 1 cut(s) 232
TfiI GAWTC 5 cut(s) 83, 278, 427, 470, 919
Tru1I TTAA 1 cut(s) 390
Tru9I TTAA 1 cut(s) 390
TscAI CASTG 4 cut(s) 229, 305, 604, 820
TseFI GTSAC 2 cut(s) 770, 811
TseI GCWGC 2 cut(s) 585, 1037
Tsp45I GTSAC 2 cut(s) 770, 811
TspDTI ATGAA 2 cut(s) 521, 803
TspGWI ACGGA 1 cut(s) 912
TspMI CCCGGG 1 cut(s) 431
TspRI CASTG 4 cut(s) 229, 305, 604, 820
VpaK11BI GGWCC 3 cut(s) 356, 435, 633
XapI RAATTY 1 cut(s) 736
XcmI CCANNNNNNNNNTGG 1 cut(s) 1050
XhoI CTCGAG 1 cut(s) 992
XmaI CCCGGG 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 756
XmnI GAANNNNTTC 1 cut(s) 842
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.