MD16G1117600.v1.1

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011253
Physical Location & Seq
Reverse (-)
8342068 .. 8345303
3236 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1117600.v1.1.491

Sequence Viewer

Length: 1161 bp
ATGCCGGCTTTTGTGAGTGAAAAGAAGAGGTTCAAAGATCCAAATATGGGCCTATCCAATTCGGTCAAGCAAGATACACCAATTCGTGAAAAAAGCTATCGCAGTTTGACTTCTCTGGTTGTAGATGATTTTGACAGCCCCATTCTGCCTGGACTGCCAGATGACATAGCAAAATATTGCCTTGCGCTTGTTCCTCGTTCAGACTTCCCTGCAATGGGTGGTGTGTGTAAGAAATGGAGGTATTTTCTTCAAAGCAAAGAACTCATCACTGTGCGGCGATTAGCTGGGTTGCTTGAGGAGTGGCTCTATGTGTTGACAACCGATTCCGAAGGAAAGGAAAACCACTGGGAGGTTTTAGATTGCCATGGACATAAGCACCATGTTCTTCCTCCCATGCCTGGTCCAATGAGATCTGGGTTTGGGGTGGTGGTTCTCAATGGAAAGCTTCTTGTCGTTGGTGGATATTCTGTGATCACCGAGACCACCGTTGCATCAGACGATGTTTACCAATATGATTCTTGCCTCAACAGGTGGGGCAAATTGGCAAACATGAATGTTGCTCGATATGACTTTGCTTGTGCAGAACTAAGTGGTATGATTTACGCAGTTGGAGGATATGGTATAGATGGCAGCAGTCTATCCAGTGCCGAGGTGTACAATCCTGACACCGATACTTGGACCCTGATAAGCAGTATTCGCCGCCCCCGCTATGGCTGCTTTGCCTGTGGATTTGAAGGAAAGCTGTATGTTATGGGCGGAAGGTCAAGCTTCACCATTGGTAATTCGAAGTTTGTTGATGTCTATGACCCTGAGAGTCACACCTGGTGCGAGATGAAGAAGAATGGTTGTGTCATGGTCACTGCTCATGCTGTGCTGGAAAAGAAGCTGTTCTGTTCGGAGTGGAAGAACCAGCGCAAACTTTCAATTTACAATCCCTACGACAATTCTTGGGAGATGGTACAGATTCCGTTGACAGGAAGCACAAGCATTAAGTTCCGATTTGGGACACTGGACGAGAAACTTTTGTTGTTCTCGCTCGAGGAGGAGCCTGGTTATCGTACTTTGTTGTACGATCCAAATGCAGCACCAGGATCAGAGTGGCAGACTTCTGAGGTAAAGATGTCCGGTCCGTGCTTGTGCTGTGTTACAATCAAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0005575 GO:0005622 GO:0005623 GO:0031461 GO:0031463 GO:0032991 GO:0044424 GO:0044464 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box Kelch_1

Protein Analysis

387

Amino Acids

43.28

Weight (kDa)

6.2

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_Calicin PF13964 129 - 276 2.5e-11 Calicin, beta-propeller domain
Kelch_1 PF01344 136 - 185 1.4e-10 Kelch motif
Beta-prop_FBX42 PF13415 140 - 283 1.9e-06 FBX42, beta-propeller domain
Kelch_KLHDC2_KLHL20_DRC7 PF24681 142 - 322 8.3e-14 KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Beta-prop_ATRN-LZTR1 PF24981 146 - 256 2.6e-09 Attractin/LZTR1 beta-propeller
Kelch_FKB95 PF25210 164 - 229 2e-07 FKB95, Kelch-repeats domain
Kelch_1 PF01344 187 - 231 2.9e-10 Kelch motif
Kelch_FKB95 PF25210 230 - 322 4.9e-10 FKB95, Kelch-repeats domain
Kelch_1 PF01344 235 - 279 1.8e-08 Kelch motif
Kelch_2 PF07646 235 - 279 9.2e-08 Kelch motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011253)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67480 AT1G67480 AT1G67480
fragaria_vesca FvH4_4g23951 FvH4_4g23951
malus_domestica MD13G1117000.v1.1 MD16G1117600.v1.1
prunus_persica Prupe.1G228600_v2.0.a1
pyrus_communis pycom13g10150
rosa_chinensis RchiOBHm_Chr4g0430611
rosa_laevigata RLG00000006963
rosa_multiflora Rmu_co8108234.1_g000001
rosa_roxburghii Rroxscaffold_5G00372360
rosa_rugosa Rorug04G0244800 Rorug04G0244800
rosa_samantha Rh4AG300700 Rh4BG307800 Rh4CG324900 Rh4DG305400
rosa_wichuraiana Rw4G026090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 274, 700, 706, 756
AclWI GGATC 3 cut(s) 32, 1067, 1099
AdeI CACNNNGTG 1 cut(s) 825
AfaI GTAC 4 cut(s) 656, 960, 1060, 1070
AfiI CCNNNNNNNGG 8 cut(s) 47, 214, 215, 349, 398, 675, 710, 974
AgsI TTSAA 4 cut(s) 34, 251, 734, 924
AjnI CCWGG 5 cut(s) 148, 397, 821, 1048, 1087
AluBI AGCT 6 cut(s) 96, 284, 445, 742, 768, 886
AluI AGCT 6 cut(s) 96, 284, 445, 742, 768, 886
Alw26I GTCTC 1 cut(s) 473
AlwI GGATC 3 cut(s) 32, 1067, 1099
Ama87I CYCGRG 1 cut(s) 1037
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 3 cut(s) 630, 714, 1082
Asp700I GAANNNNTTC 2 cut(s) 29, 887
AspLEI GCGC 2 cut(s) 187, 915
AspS9I GGNCC 4 cut(s) 49, 401, 678, 1127
AsuHPI GGTGA 2 cut(s) 466, 763
AsuII TTCGAA 1 cut(s) 785
AvaI CYCGRG 1 cut(s) 1037
AvaII GGWCC 3 cut(s) 401, 678, 1127
BbvI GCAGC 3 cut(s) 642, 701, 1094
BccI CCATC 2 cut(s) 620, 949
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 5 cut(s) 150, 399, 823, 1050, 1089
BclI TGATCA 1 cut(s) 471
BcoDI GTCTC 1 cut(s) 473
BglII AGATCT 1 cut(s) 410
BisI GCNGC 5 cut(s) 275, 631, 700, 715, 1083
BlsI GCNGC 5 cut(s) 276, 632, 701, 716, 1084
Bme1390I CCNGG 5 cut(s) 150, 399, 823, 1050, 1089
Bme18I GGWCC 3 cut(s) 401, 678, 1127
BmeT110I CYCGRG 1 cut(s) 1037
BmgT120I GGNCC 4 cut(s) 49, 401, 678, 1127
BmiI GGNNCC 2 cut(s) 680, 1047
BmrFI CCNGG 5 cut(s) 150, 399, 823, 1050, 1089
BmrI ACTGGG 1 cut(s) 355
BmsI GCATC 1 cut(s) 500
BmuI ACTGGG 1 cut(s) 355
Bpu14I TTCGAA 1 cut(s) 785
BpuEI CTTGAG 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 2 cut(s) 364, 648
BsaWI WCCGGW 1 cut(s) 1124
Bsc4I CCNNNNNNNGG 8 cut(s) 47, 214, 215, 349, 398, 675, 710, 974
Bse118I RCCGGY 1 cut(s) 4
Bse1I ACTGG 3 cut(s) 350, 642, 1014
Bse3DI GCAATG 1 cut(s) 219
BseBI CCWGG 5 cut(s) 150, 399, 823, 1050, 1089
BseDI CCNNGG 2 cut(s) 364, 648
BseLI CCNNNNNNNGG 8 cut(s) 47, 214, 215, 349, 398, 675, 710, 974
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 2 cut(s) 801, 1101
BseNI ACTGG 3 cut(s) 350, 642, 1014
BseRI GAGGAG 3 cut(s) 311, 1055, 1058
BseXI GCAGC 3 cut(s) 642, 701, 1094
BseYI CCCAGC 1 cut(s) 284
BsgI GTGCAG 1 cut(s) 600
BshFI GGCC 1 cut(s) 51
BsiHKCI CYCGRG 1 cut(s) 1037
BsiSI CCGG 2 cut(s) 5, 1125
BslFI GGGAC 1 cut(s) 1018
BslI CCNNNNNNNGG 8 cut(s) 47, 214, 215, 349, 398, 675, 710, 974
BsmAI GTCTC 1 cut(s) 473
BsmFI GGGAC 1 cut(s) 1018
BsnI GGCC 1 cut(s) 51
Bso31I GGTCTC 1 cut(s) 473
BsoBI CYCGRG 1 cut(s) 1037
Bsp119I TTCGAA 1 cut(s) 785
Bsp1407I TGTACA 1 cut(s) 654
Bsp143I GATC 5 cut(s) 37, 410, 471, 1072, 1091
Bsp19I CCATGG 1 cut(s) 364
BspACI CCGC 4 cut(s) 274, 700, 706, 756
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 2 cut(s) 802, 1102
BspLI GGNNCC 2 cut(s) 680, 1047
BspPI GGATC 3 cut(s) 32, 1067, 1099
BspT104I TTCGAA 1 cut(s) 785
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 219
BsrFI RCCGGY 1 cut(s) 4
BsrGI TGTACA 1 cut(s) 654
BsrI ACTGG 3 cut(s) 350, 642, 1014
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 364, 648
BssMI GATC 5 cut(s) 37, 410, 471, 1072, 1091
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 5 cut(s) 150, 399, 823, 1050, 1089
Bst4CI ACNGT 2 cut(s) 271, 487
Bst6I CTCTTC 1 cut(s) 20
BstAUI TGTACA 1 cut(s) 654
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 3 cut(s) 587, 810, 1110
BstDSI CCRYGG 1 cut(s) 364
BstHHI GCGC 2 cut(s) 187, 915
BstKTI GATC 5 cut(s) 40, 413, 474, 1075, 1094
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 5 cut(s) 37, 410, 471, 1072, 1091
BstMWI GCNNNNNNNGC 4 cut(s) 154, 696, 705, 714
BstNI CCWGG 5 cut(s) 150, 399, 823, 1050, 1089
BstSCI CCNGG 5 cut(s) 148, 397, 821, 1048, 1087
BstV1I GCAGC 3 cut(s) 642, 701, 1094
BstX2I RGATCY 2 cut(s) 37, 410
BstYI RGATCY 2 cut(s) 37, 410
BsuRI GGCC 1 cut(s) 51
BtgI CCRYGG 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 858
BtsIMutI CAGTG 5 cut(s) 267, 343, 649, 858, 1007
Cac8I GCNNGC 1 cut(s) 6
CfoI GCGC 2 cut(s) 187, 915
Cfr10I RCCGGY 1 cut(s) 4
Cfr13I GGNCC 4 cut(s) 49, 401, 678, 1127
CpoI CGGWCCG 1 cut(s) 1127
CsiI ACCWGGT 1 cut(s) 821
Csp6I GTAC 4 cut(s) 655, 959, 1059, 1069
CspI CGGWCCG 1 cut(s) 1127
CviAII CATG 6 cut(s) 365, 380, 394, 550, 853, 866
CviQI GTAC 4 cut(s) 655, 959, 1059, 1069
DdeI CTNAG 3 cut(s) 587, 810, 1110
DpnI GATC 5 cut(s) 39, 412, 473, 1074, 1093
DpnII GATC 5 cut(s) 37, 410, 471, 1072, 1091
DraIII CACNNNGTG 1 cut(s) 825
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 1 cut(s) 771
Eco130I CCWWGG 1 cut(s) 364
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 3 cut(s) 401, 678, 1127
Eco88I CYCGRG 1 cut(s) 1037
EcoRII CCWGG 5 cut(s) 148, 397, 821, 1048, 1087
EcoT14I CCWWGG 1 cut(s) 364
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 6 cut(s) 368, 383, 397, 553, 856, 869
FaqI GGGAC 1 cut(s) 1018
FatI CATG 6 cut(s) 364, 379, 393, 549, 852, 865
FauI CCCGC 1 cut(s) 713
FbaI TGATCA 1 cut(s) 471
Fnu4HI GCNGC 5 cut(s) 275, 631, 700, 715, 1083
Fsp4HI GCNGC 5 cut(s) 275, 631, 700, 715, 1083
GlaI GCGC 2 cut(s) 186, 914
GluI GCNGC 5 cut(s) 275, 631, 700, 715, 1083
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 1 cut(s) 51
HapII CCGG 2 cut(s) 5, 1125
HhaI GCGC 2 cut(s) 187, 915
Hin1II CATG 6 cut(s) 368, 383, 397, 553, 856, 869
Hin6I GCGC 2 cut(s) 185, 913
HinP1I GCGC 2 cut(s) 185, 913
HincII GTYRAC 2 cut(s) 315, 972
HindII GTYRAC 2 cut(s) 315, 972
HindIII AAGCTT 2 cut(s) 443, 766
HinfI GANTC 4 cut(s) 323, 515, 814, 964
HpaII CCGG 2 cut(s) 5, 1125
HphI GGTGA 2 cut(s) 466, 763
Hpy166II GTNNAC 4 cut(s) 315, 505, 655, 972
Hpy188I TCNGA 7 cut(s) 202, 328, 496, 898, 998, 1096, 1111
Hpy188III TCNNGA 2 cut(s) 86, 662
Hpy8I GTNNAC 4 cut(s) 315, 505, 655, 972
HpyAV CCTTC 3 cut(s) 323, 728, 753
HpyCH4III ACNGT 2 cut(s) 271, 487
HpyCH4V TGCA 4 cut(s) 212, 491, 581, 1082
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 696, 705, 714
HpyF3I CTNAG 3 cut(s) 587, 810, 1110
Hsp92II CATG 6 cut(s) 368, 383, 397, 553, 856, 869
HspAI GCGC 2 cut(s) 185, 913
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 5 cut(s) 37, 410, 471, 1072, 1091
LmnI GCTCC 1 cut(s) 1045
Lsp1109I GCAGC 3 cut(s) 642, 701, 1094
LweI GCATC 1 cut(s) 500
MabI ACCWGGT 1 cut(s) 821
MaeIII GTNAC 3 cut(s) 815, 856, 1144
MalI GATC 5 cut(s) 39, 412, 473, 1074, 1093
MboI GATC 5 cut(s) 37, 410, 471, 1072, 1091
MboII GAAGA 6 cut(s) 37, 239, 377, 847, 850, 916
MflI RGATCY 2 cut(s) 37, 410
MluCI AATT 6 cut(s) 58, 81, 539, 781, 924, 943
MlyI GAGTC 1 cut(s) 823
MmeI TCCRAC 1 cut(s) 589
MroNI GCCGGC 1 cut(s) 4
MroXI GAANNNNTTC 2 cut(s) 29, 887
MseI TTAA 1 cut(s) 990
MslI CAYNNNNRTG 1 cut(s) 269
MspI CCGG 2 cut(s) 5, 1125
MspR9I CCNGG 5 cut(s) 150, 399, 823, 1050, 1089
MvaI CCWGG 5 cut(s) 150, 399, 823, 1050, 1089
MwoI GCNNNNNNNGC 4 cut(s) 154, 696, 705, 714
NaeI GCCGGC 1 cut(s) 6
NcoI CCATGG 1 cut(s) 364
NdeII GATC 5 cut(s) 37, 410, 471, 1072, 1091
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 6 cut(s) 368, 383, 397, 553, 856, 869
NlaIV GGNNCC 2 cut(s) 680, 1047
NmeAIII GCCGAG 1 cut(s) 673
NmuCI GTSAC 2 cut(s) 815, 856
NspV TTCGAA 1 cut(s) 785
PaeR7I CTCGAG 1 cut(s) 1037
PdiI GCCGGC 1 cut(s) 6
PdmI GAANNNNTTC 2 cut(s) 29, 887
PfeI GAWTC 3 cut(s) 323, 515, 964
PkrI GCNGC 5 cut(s) 276, 632, 701, 716, 1084
PleI GAGTC 1 cut(s) 822
PpsI GAGTC 1 cut(s) 822
Psp6I CCWGG 5 cut(s) 148, 397, 821, 1048, 1087
PspFI CCCAGC 1 cut(s) 284
PspGI CCWGG 5 cut(s) 148, 397, 821, 1048, 1087
PspN4I GGNNCC 2 cut(s) 680, 1047
PspPI GGNCC 4 cut(s) 49, 401, 678, 1127
PspXI VCTCGAGB 1 cut(s) 1037
PsuI RGATCY 2 cut(s) 37, 410
RsaI GTAC 4 cut(s) 656, 960, 1060, 1070
RsaNI GTAC 4 cut(s) 655, 959, 1059, 1069
RseI CAYNNNNRTG 1 cut(s) 269
Rsr2I CGGWCCG 1 cut(s) 1127
RsrII CGGWCCG 1 cut(s) 1127
SaqAI TTAA 1 cut(s) 990
SatI GCNGC 5 cut(s) 275, 631, 700, 715, 1083
Sau3AI GATC 5 cut(s) 37, 410, 471, 1072, 1091
Sau96I GGNCC 4 cut(s) 49, 401, 678, 1127
SchI GAGTC 1 cut(s) 823
ScrFI CCNGG 5 cut(s) 150, 399, 823, 1050, 1089
SexAI ACCWGGT 1 cut(s) 821
SfaNI GCATC 1 cut(s) 500
Sfr274I CTCGAG 1 cut(s) 1037
SfuI TTCGAA 1 cut(s) 785
SinI GGWCC 3 cut(s) 401, 678, 1127
SlaI CTCGAG 1 cut(s) 1037
SmiMI CAYNNNNRTG 1 cut(s) 269
SmlI CTYRAG 2 cut(s) 293, 1037
SmoI CTYRAG 2 cut(s) 293, 1037
Sse9I AATT 6 cut(s) 58, 81, 539, 781, 924, 943
SsiI CCGC 4 cut(s) 274, 700, 706, 756
SspI AATATT 1 cut(s) 176
StyD4I CCNGG 5 cut(s) 148, 397, 821, 1048, 1087
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 2 cut(s) 271, 487
TaqI TCGA 3 cut(s) 562, 785, 1038
TaqII GACCGA 1 cut(s) 52
TasI AATT 6 cut(s) 58, 81, 539, 781, 924, 943
TatI WGTACW 1 cut(s) 654
TauI GCSGC 2 cut(s) 277, 702
TfiI GAWTC 3 cut(s) 323, 515, 964
Tru1I TTAA 1 cut(s) 990
Tru9I TTAA 1 cut(s) 990
TscAI CASTG 5 cut(s) 274, 350, 649, 865, 1014
TseFI GTSAC 2 cut(s) 815, 856
TseI GCWGC 3 cut(s) 630, 714, 1082
Tsp45I GTSAC 2 cut(s) 815, 856
TspDTI ATGAA 2 cut(s) 566, 848
TspGWI ACGGA 2 cut(s) 957, 1119
TspRI CASTG 5 cut(s) 274, 350, 649, 865, 1014
VpaK11BI GGWCC 3 cut(s) 401, 678, 1127
XcmI CCANNNNNNNNNTGG 1 cut(s) 1095
XhoI CTCGAG 1 cut(s) 1037
XmnI GAANNNNTTC 2 cut(s) 29, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.