Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb4

Orthogroup ID

OG0012815

Physical Location & Seq

Genomic Coordinates Reverse (-)

27198170 .. 27199644

Gene Structure 1475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_4g25420.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1371 bp

ATGACCCACTACGTGAAGACCGGCCTCATCGACCACGCCCTCCACGTGTTCGACGAAATGACCCAATCAAACTGCCGCGTCTTCAGCGTCGACTACAATCGTTTCATCGGCGTCCTCGTCAAGCACTCCCGTTACGACCTCGCCGAGCATTACTACTACGAGATGGCCCCACAGGGCTTCTCCCTAACCCCCTTCACTTACTCCAGGTTCATTTCCGGCCTGTGTAATGTAAACAACTTTACGTTGATCGAGAAGCTTCTAAGGGACATGGATAGATTAGGTTCCGTCCCCGATATTTGGGCTTTTAATATTTACTTGAATTTACTTTGCCAAGAAAATAGGATACATTATGCTCTGGAGGTGTTTGATAGGATGGCGGACAAGGGGAGGGAGCCGGATGTTGTGTCGTATACTATTGTGATTCATGGGTTGTGTAAAGCTGGGAGGTTCGATAATGCGGTTGAGATTTGGAATGGTATGATTAGTAAAGGGTTTAGGCCTGATACTATTGCGTGCACCGCGCTTGTTGTTGGGTTGTGTGGAGGTGGGAAGGTTGATTTGGCTTATGACCTTGTGATTGGCGAGATGAAGGGTGGTGTGGAGTTTAATAATTTGATTTACAACGCGCTGATTAGTGGGTTTTGTCGAGCGAGGAGGATTGACAAGGCGCAGGCGATTAAGTTGTTTATGAAGAGGAATGGGTGCAAGCCGGATTTGGTGACGCATAATGTGTTGTTGAATTATTGTTGTAATGAGTTCATGTTGGAGGAGGCGGAGAAGTTGATGAGACAAATGGAGAAGATTGGGATGAAGCTGGATGCGTATAGTTATAATGGGTTTCTTAAGGGTCTTTGTAAAGATAATAGACCCGAAAAGGCGTATTTGTTTATGAAGAACATGATGGAGCCTAAAGGTTTGTGTAATGTTGTTTCATTTAATACCATTATTATGGCGTGTTGCAAGGGAGGCCAAACTAGAAGAGCATACAAACTGTTTCAGGAAATGTTTCAAAAGGGAATATTACCGGATGTTGTTACATTTACTATTCTGATAGGAGCTTTTTTAAGAGAAGGTAGTTCAGATGTAGCCAAGAAGCTTCTTGATCAGATGACAGGGATGGATCTGTTGCCTGATCGTATATTTTACACTACAATAATTGATCATCTATGTAAGAGTGGGAAGATTGCAATGGCTTGTGACATGTTCAATGATATGTTAGAGAAGGGAATCACCGCTGATGTGGTTTTATATAATGCTCTCATTAGCGGGCTTTGTAGGGCTTCTAGAGTTACTGAAGCCATGCATTTATATGAGGAAATGCAGACTAGAGGATGCTTTCCTGATGAGAGTTCTTTCCGAGACACTGATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031090 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0098588 GO:0098805 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

457

Amino Acids

51.9

Weight (kDa)

6.79

Isoelectric Point (pI)

30.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	65 - 111	5.6e-06	PPR repeat family
PPR_3	PF13812	85 - 141	5.8e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	97 - 146	1.2e-14	PPR repeat family
PPR_1	PF12854	128 - 160	1.3e-11	PPR repeat
PPR_2	PF13041	132 - 180	3.6e-13	PPR repeat family
PPR	PF01535	135 - 165	8e-08	PPR repeat
PPR_3	PF13812	198 - 251	6.4e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	199 - 225	2e-06	PPR repeat
PPR_2	PF13041	205 - 251	4.1e-13	PPR repeat family
PPR_1	PF12854	233 - 266	1.1e-07	PPR repeat
PPR_2	PF13041	240 - 286	2.3e-11	PPR repeat family
PPR_long	PF17177	245 - 379	3e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	305 - 335	4.1e-08	PPR repeat
PPR_2	PF13041	308 - 355	1e-16	PPR repeat family
PPR	PF01535	310 - 340	8.9e-08	PPR repeat
PPR_3	PF13812	330 - 387	3.8e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	338 - 371	8.8e-08	PPR repeat
PPR_2	PF13041	342 - 378	1e-05	PPR repeat family
TPR_24	PF23276	345 - 440	1.4e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	374 - 405	8.1e-07	PPR repeat
PPR_2	PF13041	377 - 415	1e-09	PPR repeat family
PPR	PF01535	382 - 410	5.8e-06	PPR repeat
PPR_1	PF12854	408 - 441	1.5e-10	PPR repeat
PPR_2	PF13041	413 - 452	2.6e-12	PPR repeat family
PPR	PF01535	415 - 445	2.3e-08	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13040 AT1G13040
fragaria_vesca	FvH4_4g25420
malus_domestica	MD13G1103100.v1.1 MD16G1103300.v1.1
prunus_persica	Prupe.1G242700_v2.0.a1
pyrus_communis	pycom13g09010 pycom16g08810
rosa_chinensis	RchiOBHm_Chr4g0432731
rosa_laevigata	RLG00000006817
rosa_multiflora	Rmu_co8522421.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374190
rosa_rugosa	Rorug04G0262000
rosa_samantha	Rh4AG316500 Rh4BG324500 Rh4CG339500 Rh4DG319800
rosa_wichuraiana	Rw4G027530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	831
AccI	GTMKAC	2 cut(s)	90, 410
AccII	CGCG	3 cut(s)	78, 521, 626
AciI	CCGC	7 cut(s)	76, 377, 458, 519, 773, 1233, 1266
AclWI	GGATC	1 cut(s)	1128
AcsI	RAATTY	1 cut(s)	319
AcuI	CTGAAG	2 cut(s)	67, 1314
AcvI	CACGTG	1 cut(s)	46
AcyI	GRCGYC	1 cut(s)	111
AdeI	CACNNNGTG	1 cut(s)	13
AfiI	CCNNNNNNNGG	1 cut(s)	297
AflII	CTTAAG	1 cut(s)	842
AflIII	ACRYGT	2 cut(s)	45, 1200
AgsI	TTSAA	4 cut(s)	319, 739, 1010, 1207
AjnI	CCWGG	1 cut(s)	203
AjuI	GAANNNNNNNTTGG	2 cut(s)	542, 574
AluBI	AGCT	5 cut(s)	256, 440, 814, 1058, 1096
AluI	AGCT	5 cut(s)	256, 440, 814, 1058, 1096
Alw21I	GWGCWC	1 cut(s)	518
Alw26I	GTCTC	2 cut(s)	781, 1353
Alw44I	GTGCAC	1 cut(s)	514
AlwI	GGATC	1 cut(s)	1128
AoxI	GGCC	5 cut(s)	22, 165, 217, 497, 967
ApaLI	GTGCAC	1 cut(s)	514
ApoI	RAATTY	1 cut(s)	319
Asp700I	GAANNNNTTC	1 cut(s)	1005
AspLEI	GCGC	3 cut(s)	523, 628, 670
AspS9I	GGNCC	1 cut(s)	166
AsuHPI	GGTGA	2 cut(s)	730, 1222
BaeGI	GKGCMC	1 cut(s)	518
BbrPI	CACGTG	1 cut(s)	46
BbsI	GAAGAC	2 cut(s)	23, 73
Bbv12I	GWGCWC	1 cut(s)	518
BccI	CCATC	4 cut(s)	157, 367, 895, 1111
BciT130I	CCWGG	1 cut(s)	205
BciVI	GTATCC	1 cut(s)	336
BclI	TGATCA	2 cut(s)	1102, 1159
BcoDI	GTCTC	2 cut(s)	781, 1353
BfaI	CTAG	3 cut(s)	975, 1284, 1326
BfrI	CTTAAG	1 cut(s)	842
BfuI	GTATCC	1 cut(s)	336
BisI	GCNGC	1 cut(s)	76
BlsI	GCNGC	1 cut(s)	77
Bme1390I	CCNGG	1 cut(s)	205
BmgT120I	GGNCC	1 cut(s)	166
BmiI	GGNNCC	4 cut(s)	168, 283, 393, 906
BmrFI	CCNGG	1 cut(s)	205
BmsI	GCATC	2 cut(s)	808, 1322
BpiI	GAAGAC	2 cut(s)	23, 73
BpmI	CTGGAG	2 cut(s)	187, 377
BsaAI	YACGTR	2 cut(s)	13, 46
BsaHI	GRCGYC	1 cut(s)	111
BsaWI	WCCGGW	1 cut(s)	1024
Bsc4I	CCNNNNNNNGG	1 cut(s)	297
Bse118I	RCCGGY	1 cut(s)	20
Bse3DI	GCAATG	1 cut(s)	1194
BseBI	CCWGG	1 cut(s)	205
BseGI	GGATG	7 cut(s)	378, 403, 813, 823, 1033, 1122, 1337
BseLI	CCNNNNNNNGG	1 cut(s)	297
BseMI	GCAATG	1 cut(s)	1194
BseRI	GAGGAG	2 cut(s)	667, 782
BseSI	GKGCMC	1 cut(s)	518
BseYI	CCCAGC	1 cut(s)	440
Bsh1236I	CGCG	3 cut(s)	78, 521, 626
BshFI	GGCC	5 cut(s)	24, 167, 219, 499, 969
BsiHKAI	GWGCWC	1 cut(s)	518
BsiSI	CCGG	5 cut(s)	21, 216, 395, 710, 1025
BslFI	GGGAC	2 cut(s)	272, 278
BslI	CCNNNNNNNGG	1 cut(s)	297
BsmAI	GTCTC	2 cut(s)	781, 1353
BsmFI	GGGAC	2 cut(s)	272, 278
BsnI	GGCC	5 cut(s)	24, 167, 219, 499, 969
Bsp1286I	GDGCHC	1 cut(s)	518
Bsp143I	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
BspACI	CCGC	7 cut(s)	76, 377, 458, 519, 773, 1233, 1266
BspANI	GGCC	5 cut(s)	24, 167, 219, 499, 969
BspFNI	CGCG	3 cut(s)	78, 521, 626
BspLI	GGNNCC	4 cut(s)	168, 283, 393, 906
BspPI	GGATC	1 cut(s)	1128
BspQI	GCTCTTC	1 cut(s)	973
BspTI	CTTAAG	1 cut(s)	842
BsrDI	GCAATG	1 cut(s)	1194
BsrFI	RCCGGY	1 cut(s)	20
BssAI	RCCGGY	1 cut(s)	20
BssMI	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
BssNAI	GTATAC	1 cut(s)	411
BssNI	GRCGYC	1 cut(s)	111
Bst1107I	GTATAC	1 cut(s)	411
Bst2UI	CCWGG	1 cut(s)	205
Bst4CI	ACNGT	1 cut(s)	993
Bst6I	CTCTTC	2 cut(s)	686, 973
BstACI	GRCGYC	1 cut(s)	111
BstAFI	CTTAAG	1 cut(s)	842
BstBAI	YACGTR	2 cut(s)	13, 46
BstC8I	GCNNGC	4 cut(s)	514, 672, 707, 1268
BstDEI	CTNAG	1 cut(s)	260
BstF5I	GGATG	7 cut(s)	378, 403, 813, 823, 1033, 1122, 1337
BstFNI	CGCG	3 cut(s)	78, 521, 626
BstHHI	GCGC	3 cut(s)	523, 628, 670
BstKTI	GATC	5 cut(s)	249, 1105, 1123, 1135, 1162
BstMAI	GTCTC	2 cut(s)	781, 1353
BstMBI	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
BstMWI	GCNNNNNNNGC	3 cut(s)	84, 518, 966
BstNI	CCWGG	1 cut(s)	205
BstNSI	RCATGY	1 cut(s)	1204
BstSCI	CCNGG	1 cut(s)	203
BstSLI	GKGCMC	1 cut(s)	518
BstUI	CGCG	3 cut(s)	78, 521, 626
BstV2I	GAAGAC	2 cut(s)	23, 73
BstX2I	RGATCY	1 cut(s)	1120
BstXI	CCANNNNNNTGG	1 cut(s)	949
BstYI	RGATCY	1 cut(s)	1120
BstZ17I	GTATAC	1 cut(s)	411
BsuI	GTATCC	1 cut(s)	336
BsuRI	GGCC	5 cut(s)	24, 167, 219, 499, 969
BtsCI	GGATG	7 cut(s)	378, 403, 813, 823, 1033, 1122, 1337
BtsIMutI	CAGTG	1 cut(s)	1362
Cac8I	GCNNGC	4 cut(s)	514, 672, 707, 1268
CfoI	GCGC	3 cut(s)	523, 628, 670
Cfr10I	RCCGGY	1 cut(s)	20
Cfr13I	GGNCC	1 cut(s)	166
CseI	GACGC	4 cut(s)	67, 76, 100, 730
CviAII	CATG	6 cut(s)	268, 425, 760, 898, 1201, 1300
DdeI	CTNAG	1 cut(s)	260
DpnI	GATC	5 cut(s)	248, 1104, 1122, 1134, 1161
DpnII	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
DraIII	CACNNNGTG	1 cut(s)	13
Eam1104I	CTCTTC	2 cut(s)	686, 973
EarI	CTCTTC	2 cut(s)	686, 973
EciI	GGCGGA	2 cut(s)	392, 788
Eco147I	AGGCCT	1 cut(s)	499
Eco57I	CTGAAG	2 cut(s)	67, 1314
Eco72I	CACGTG	1 cut(s)	46
EcoRII	CCWGG	1 cut(s)	203
EcoT22I	ATGCAT	1 cut(s)	1305
FaeI	CATG	6 cut(s)	271, 428, 763, 901, 1204, 1303
FaqI	GGGAC	2 cut(s)	272, 278
FatI	CATG	6 cut(s)	267, 424, 759, 897, 1200, 1299
FauI	CCCGC	1 cut(s)	1259
FbaI	TGATCA	2 cut(s)	1102, 1159
FblI	GTMKAC	2 cut(s)	90, 410
Fnu4HI	GCNGC	1 cut(s)	76
FokI	GGATG	7 cut(s)	385, 410, 820, 830, 1040, 1129, 1344
Fsp4HI	GCNGC	1 cut(s)	76
FspBI	CTAG	3 cut(s)	975, 1284, 1326
GlaI	GCGC	3 cut(s)	522, 627, 669
GluI	GCNGC	1 cut(s)	76
GsaI	CCCAGC	1 cut(s)	444
GsuI	CTGGAG	2 cut(s)	187, 377
HaeIII	GGCC	5 cut(s)	24, 167, 219, 499, 969
HapII	CCGG	5 cut(s)	21, 216, 395, 710, 1025
HgaI	GACGC	4 cut(s)	67, 76, 100, 730
HhaI	GCGC	3 cut(s)	523, 628, 670
Hin1I	GRCGYC	1 cut(s)	111
Hin1II	CATG	6 cut(s)	271, 428, 763, 901, 1204, 1303
Hin6I	GCGC	3 cut(s)	521, 626, 668
HinP1I	GCGC	3 cut(s)	521, 626, 668
HincII	GTYRAC	1 cut(s)	91
HindII	GTYRAC	1 cut(s)	91
HindIII	AAGCTT	2 cut(s)	254, 1094
HinfI	GANTC	2 cut(s)	421, 1227
HpaII	CCGG	5 cut(s)	21, 216, 395, 710, 1025
HphI	GGTGA	2 cut(s)	730, 1222
Hpy166II	GTNNAC	4 cut(s)	91, 232, 411, 516
Hpy188I	TCNGA	4 cut(s)	1050, 1081, 1107, 1358
Hpy188III	TCNNGA	6 cut(s)	250, 356, 998, 1100, 1284, 1340
Hpy8I	GTNNAC	4 cut(s)	91, 232, 411, 516
Hpy99I	CGWCG	2 cut(s)	56, 92
HpyAV	CCTTC	5 cut(s)	202, 544, 583, 1064, 1216
HpyCH4III	ACNGT	1 cut(s)	993
HpyCH4IV	ACGT	3 cut(s)	12, 45, 242
HpyCH4V	TGCA	6 cut(s)	516, 705, 960, 1187, 1303, 1321
HpyF10VI	GCNNNNNNNGC	3 cut(s)	84, 518, 966
HpyF3I	CTNAG	1 cut(s)	260
HpySE526I	ACGT	3 cut(s)	12, 45, 242
Hsp92I	GRCGYC	1 cut(s)	111
Hsp92II	CATG	6 cut(s)	271, 428, 763, 901, 1204, 1303
HspAI	GCGC	3 cut(s)	521, 626, 668
Ksp22I	TGATCA	2 cut(s)	1102, 1159
Kzo9I	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
LguI	GCTCTTC	1 cut(s)	973
LmnI	GCTCC	3 cut(s)	391, 904, 1055
LweI	GCATC	2 cut(s)	808, 1322
MaeI	CTAG	3 cut(s)	975, 1284, 1326
MaeII	ACGT	3 cut(s)	12, 45, 242
MaeIII	GTNAC	5 cut(s)	131, 718, 1033, 1196, 1288
MalI	GATC	5 cut(s)	248, 1104, 1122, 1134, 1161
MboI	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
MboII	GAAGA	7 cut(s)	28, 73, 703, 811, 904, 990, 1192
MflI	RGATCY	1 cut(s)	1120
MhlI	GDGCHC	1 cut(s)	518
MluCI	AATT	4 cut(s)	319, 610, 739, 1155
MmeI	TCCRAC	1 cut(s)	744
Mph1103I	ATGCAT	1 cut(s)	1305
MroXI	GAANNNNTTC	1 cut(s)	1005
MseI	TTAA	7 cut(s)	306, 606, 678, 843, 936, 1064, 1369
MslI	CAYNNNNRTG	2 cut(s)	947, 1308
MspA1I	CMGCKG	1 cut(s)	1235
MspCI	CTTAAG	1 cut(s)	842
MspI	CCGG	5 cut(s)	21, 216, 395, 710, 1025
MspR9I	CCNGG	1 cut(s)	205
MvaI	CCWGG	1 cut(s)	205
MvnI	CGCG	3 cut(s)	78, 521, 626
MwoI	GCNNNNNNNGC	3 cut(s)	84, 518, 966
NdeII	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
NlaIII	CATG	6 cut(s)	271, 428, 763, 901, 1204, 1303
NlaIV	GGNNCC	4 cut(s)	168, 283, 393, 906
NmeAIII	GCCGAG	1 cut(s)	169
NmuCI	GTSAC	2 cut(s)	718, 1196
NsiI	ATGCAT	1 cut(s)	1305
NspI	RCATGY	1 cut(s)	1204
PceI	AGGCCT	1 cut(s)	499
PciI	ACATGT	1 cut(s)	1200
PciSI	GCTCTTC	1 cut(s)	973
PcsI	WCGNNNNNNNCGW	4 cut(s)	42, 51, 114, 141
PdmI	GAANNNNTTC	1 cut(s)	1005
PfeI	GAWTC	2 cut(s)	421, 1227
PkrI	GCNGC	1 cut(s)	77
PmaCI	CACGTG	1 cut(s)	46
PmlI	CACGTG	1 cut(s)	46
Ppu21I	YACGTR	2 cut(s)	13, 46
PscI	ACATGT	1 cut(s)	1200
PsiI	TTATAA	1 cut(s)	831
Psp6I	CCWGG	1 cut(s)	203
PspCI	CACGTG	1 cut(s)	46
PspFI	CCCAGC	1 cut(s)	440
PspGI	CCWGG	1 cut(s)	203
PspN4I	GGNNCC	4 cut(s)	168, 283, 393, 906
PspPI	GGNCC	1 cut(s)	166
PsuI	RGATCY	1 cut(s)	1120
RseI	CAYNNNNRTG	2 cut(s)	947, 1308
SalI	GTCGAC	1 cut(s)	89
SapI	GCTCTTC	1 cut(s)	973
SaqAI	TTAA	7 cut(s)	306, 606, 678, 843, 936, 1064, 1369
SatI	GCNGC	1 cut(s)	76
Sau3AI	GATC	5 cut(s)	246, 1102, 1120, 1132, 1159
Sau96I	GGNCC	1 cut(s)	166
ScrFI	CCNGG	1 cut(s)	205
SduI	GDGCHC	1 cut(s)	518
SfaNI	GCATC	2 cut(s)	808, 1322
SmiMI	CAYNNNNRTG	2 cut(s)	947, 1308
SmlI	CTYRAG	1 cut(s)	842
SmoI	CTYRAG	1 cut(s)	842
Sse9I	AATT	4 cut(s)	319, 610, 739, 1155
SseBI	AGGCCT	1 cut(s)	499
SsiI	CCGC	7 cut(s)	76, 377, 458, 519, 773, 1233, 1266
SspI	AATATT	2 cut(s)	310, 1020
SspMI	CTAG	3 cut(s)	975, 1284, 1326
StuI	AGGCCT	1 cut(s)	499
StyD4I	CCNGG	1 cut(s)	203
TaaI	ACNGT	1 cut(s)	993
TaiI	ACGT	3 cut(s)	15, 48, 245
TaqI	TCGA	6 cut(s)	30, 51, 90, 249, 450, 646
TasI	AATT	4 cut(s)	319, 610, 739, 1155
TauI	GCSGC	1 cut(s)	78
TfiI	GAWTC	2 cut(s)	421, 1227
Tru1I	TTAA	7 cut(s)	306, 606, 678, 843, 936, 1064, 1369
Tru9I	TTAA	7 cut(s)	306, 606, 678, 843, 936, 1064, 1369
TscAI	CASTG	1 cut(s)	1369
TseFI	GTSAC	2 cut(s)	718, 1196
Tsp45I	GTSAC	2 cut(s)	718, 1196
TspDTI	ATGAA	9 cut(s)	94, 199, 413, 602, 704, 748, 824, 905, 921
TspGWI	ACGGA	1 cut(s)	274
TspRI	CASTG	1 cut(s)	1369
Vha464I	CTTAAG	1 cut(s)	842
VneI	GTGCAC	1 cut(s)	514
XapI	RAATTY	1 cut(s)	319
XbaI	TCTAGA	1 cut(s)	1283
XceI	RCATGY	1 cut(s)	1204
XmiI	GTMKAC	2 cut(s)	90, 410
XmnI	GAANNNNTTC	1 cut(s)	1005
XspI	CTAG	3 cut(s)	975, 1284, 1326
Zsp2I	ATGCAT	1 cut(s)	1305

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.