Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012815

Physical Location & Seq

Genomic Coordinates Forward (+)

7363825 .. 7367391

Gene Structure 3567 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1103100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGTATCGTAGTCTTGGCGTTCACCGCCTCATATACCGGAGCCGCATAGTGTACTACGTGAAAACTGGCCTCATCGATCAAGCACTACAGGTGTTCGACGAAATGACCCACTCGGACTGCCGGGTCTTCAGCGTCGACTACAACCGCTTCATCGGCGTACTCGTCAAGCACTCGCGGTACGATCTCGCCGAGCACTACTACTACGAAATGGTGCCGAAGGGGTTCTCATTGGACCCCTTTACTTACTCAAGGTTCATTTCTGGCTTGTGCAAGATTAGAAATTTCACCCTCATTGAGAAGCTTCTTCGAGACATGGATAGGATTGGGGCATTGCCTGACATCTGGGCTTTCAATATATATTTGAATCTTCTGTGCCAAGAAGACAGGATGGATTTCGCTTTGGAATTGCTTCATAGGATGGTTGACAAAGGAAGAGAGCCGGATGGTGTATCGTATACTATAATTATTGATGGGTTGTGTAAAGCTGGACAATTTGATACGGCAGTTGATATTTGGAATGGTATGATTAAGAAAGGGTTCAGGCCTGATAACATTGCGTGCACGGCGCTGGTTGTTGGGTTGTGCAAGGGTGGGAAGGTTGATTTGGCTTATGACCTTGTCATTGATGAAGTGAAGGGTAGTGTTAATTTTAGTAGTTTGATTTATAATGCGTTGATTAGTGGGTTTTGTCGTGCCGGTAGGATTGATAAGGCACAAGCAATCAAGTCATTTATGAAGAGAAATGGTTGCGAGCCAGATTTGGTTACTCACAATGTGTTGTTGAATTATTGTTGTAATCAGTTCATGTTGGAGGAGGCTGAGAAGTTGATGAAGAAAATGGAGAGGGGTGGGATGGAACCTGATGTGTATAGTTATAATCAGCTTCTCATGGGCCTTTGTAAAGTTAATAGACCGGATAAGGCGTATTTGTTGATGAGAACTAGGATGGAGCCAAAGGGGTTGTGTAATGTTGTGTCGTACAATACCATCATTACATCATTTTGCAAGGCAAGCCGTACCCGAAGTGCCTACAAATTGTTTGAGGAAATGCGTCACAAGGGCACTGTGCCAGATTTGGTTACATTCACTATTCTTATAAATGCCTTTTTAAGAGAAGGTAGTTCAGAGATAGCCAAGAAGCTTCTTGATCAGACAACAGCGATGGGTCTGTTGCTTGACTGTATATTTTACACTACAATAGTTGATCACCTATGTAAGAATGGGAAGATTGAAATGGCTTATAGTGTTTTCAGTGAAATGTTTGAGAAGGGAATCACCCCTGATGTGATTTCATATAATGCTCTCATTAACGGGCTGCTCAAGGCTTCTAAAGTGAGTGACGCTATGCATCTCTACGAGGAAATGCAGACTAGAGGGTGTAGTGCTGACGGGGTAACTTTTAAATTGATAATTGGAGGTCTCATAAGGGAGAATAAGCTGTCAGTCGCTTGTAGGGTATGGGATCAGATGATGGAAAAGGGCTTTACTCTTGATGGAGCTTTCTCCAAGACTCTGGTTAATGCCATCAATTCAAAAGATGCTGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031090 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0098588 GO:0098805 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

516

Amino Acids

58.47

Weight (kDa)

8.31

Isoelectric Point (pI)

23.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	47 - 91	4.8e-07	PPR repeat family
PPR_2	PF13041	112 - 161	8.7e-14	PPR repeat family
PPR_1	PF12854	143 - 175	3.6e-09	PPR repeat
PPR_2	PF13041	150 - 196	2.4e-12	PPR repeat family
PPR	PF01535	150 - 180	1.6e-07	PPR repeat
PPR_3	PF13812	215 - 266	3.5e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	220 - 266	1.9e-14	PPR repeat family
PPR	PF01535	221 - 250	1.7e-06	PPR repeat
PPR_1	PF12854	221 - 245	1.5e-07	PPR repeat
PPR_1	PF12854	248 - 281	7.7e-07	PPR repeat
PPR_2	PF13041	253 - 301	9.1e-14	PPR repeat family
PPR_1	PF12854	284 - 313	8.2e-07	PPR repeat
PPR_1	PF12854	320 - 351	1.6e-10	PPR repeat
PPR_3	PF13812	322 - 369	7.3e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	323 - 370	3e-17	PPR repeat family
PPR	PF01535	325 - 354	3.3e-07	PPR repeat
TPR_24	PF23276	371 - 489	1.6e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	380 - 437	2.9e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	388 - 420	4.8e-07	PPR repeat
PPR_2	PF13041	395 - 441	1.7e-15	PPR repeat family
PPR	PF01535	397 - 425	1.6e-06	PPR repeat
PPR_1	PF12854	423 - 456	2.7e-08	PPR repeat
PPR_2	PF13041	427 - 475	3.8e-14	PPR repeat family
PPR	PF01535	430 - 460	4.1e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13040 AT1G13040
fragaria_vesca	FvH4_4g25420
malus_domestica	MD13G1103100.v1.1 MD16G1103300.v1.1
prunus_persica	Prupe.1G242700_v2.0.a1
pyrus_communis	pycom13g09010 pycom16g08810
rosa_chinensis	RchiOBHm_Chr4g0432731
rosa_laevigata	RLG00000006817
rosa_multiflora	Rmu_co8522421.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374190
rosa_rugosa	Rorug04G0262000
rosa_samantha	Rh4AG316500 Rh4BG324500 Rh4CG339500 Rh4DG319800
rosa_wichuraiana	Rw4G027530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	666, 876, 1097
AasI	GACNNNNNNGTC	1 cut(s)	122
AccB1I	GGYRCC	1 cut(s)	211
AccI	GTMKAC	2 cut(s)	135, 455
AccII	CGCG	1 cut(s)	175
AciI	CCGC	4 cut(s)	25, 43, 145, 175
AclWI	GGATC	1 cut(s)	1470
AcsI	RAATTY	1 cut(s)	280
AcuI	CTGAAG	1 cut(s)	112
AdeI	CACNNNGTG	1 cut(s)	775
AfaI	GTAC	5 cut(s)	53, 159, 179, 980, 1018
AgsI	TTSAA	5 cut(s)	352, 364, 784, 1232, 1533
AjuI	GAANNNNNNNTTGG	2 cut(s)	587, 619
AluBI	AGCT	6 cut(s)	301, 485, 883, 1141, 1438, 1499
AluI	AGCT	6 cut(s)	301, 485, 883, 1141, 1438, 1499
Alw21I	GWGCWC	2 cut(s)	195, 563
Alw26I	GTCTC	2 cut(s)	303, 1424
Alw44I	GTGCAC	1 cut(s)	559
AlwI	GGATC	1 cut(s)	1470
AoxI	GGCC	3 cut(s)	67, 542, 892
ApaLI	GTGCAC	1 cut(s)	559
ApeKI	GCWGC	2 cut(s)	1315, 1541
ApoI	RAATTY	1 cut(s)	280
Asp700I	GAANNNNTTC	3 cut(s)	221, 408, 536
AspLEI	GCGC	1 cut(s)	568
AspS9I	GGNCC	2 cut(s)	232, 892
AsuC2I	CCSGG	1 cut(s)	122
AsuHPI	GGTGA	4 cut(s)	14, 277, 1199, 1267
AvaII	GGWCC	1 cut(s)	232
BaeGI	GKGCMC	2 cut(s)	563, 1064
BaeI	ACNNNNGTAYC	2 cut(s)	489, 522
BanI	GGYRCC	1 cut(s)	211
BbsI	GAAGAC	2 cut(s)	118, 387
Bbv12I	GWGCWC	2 cut(s)	195, 563
BbvI	GCAGC	2 cut(s)	1302, 1528
BceAI	ACGGC	3 cut(s)	516, 579, 999
BclI	TGATCA	2 cut(s)	1147, 1204
BcnI	CCSGG	1 cut(s)	122
BcoDI	GTCTC	2 cut(s)	303, 1424
BfaI	CTAG	2 cut(s)	942, 1371
BfmI	CTRYAG	1 cut(s)	86
BfoI	RGCGCY	1 cut(s)	569
BisI	GCNGC	3 cut(s)	43, 1316, 1542
BlsI	GCNGC	3 cut(s)	44, 1317, 1543
Bme1390I	CCNGG	1 cut(s)	122
Bme18I	GGWCC	1 cut(s)	232
BmgT120I	GGNCC	2 cut(s)	232, 892
BmiI	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
BmrFI	CCNGG	1 cut(s)	122
BmsI	GCATC	2 cut(s)	1357, 1528
BpiI	GAAGAC	2 cut(s)	118, 387
BpuEI	CTTGAG	2 cut(s)	232, 1304
BpuMI	CCSGG	1 cut(s)	122
Bsa29I	ATCGAT	1 cut(s)	75
BsaAI	YACGTR	1 cut(s)	58
BsaI	GGTCTC	1 cut(s)	1424
BsaWI	WCCGGW	2 cut(s)	36, 913
Bse118I	RCCGGY	1 cut(s)	695
Bse1I	ACTGG	1 cut(s)	70
Bse3DI	GCAATG	2 cut(s)	329, 552
BseCI	ATCGAT	1 cut(s)	75
BseGI	GGATG	5 cut(s)	393, 423, 448, 858, 951
BseMI	GCAATG	2 cut(s)	329, 552
BseMII	CTCAG	1 cut(s)	810
BseNI	ACTGG	1 cut(s)	70
BseRI	GAGGAG	1 cut(s)	827
BseSI	GKGCMC	2 cut(s)	563, 1064
BseXI	GCAGC	2 cut(s)	1302, 1528
Bsh1236I	CGCG	1 cut(s)	175
BshFI	GGCC	3 cut(s)	69, 544, 894
BshNI	GGYRCC	1 cut(s)	211
BshVI	ATCGAT	1 cut(s)	75
BsiHKAI	GWGCWC	2 cut(s)	195, 563
BsiSI	CCGG	5 cut(s)	37, 121, 440, 696, 914
BsmAI	GTCTC	2 cut(s)	303, 1424
BsnI	GGCC	3 cut(s)	69, 544, 894
Bso31I	GGTCTC	1 cut(s)	1424
Bsp1286I	GDGCHC	3 cut(s)	195, 563, 1064
Bsp143I	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BspACI	CCGC	4 cut(s)	25, 43, 145, 175
BspANI	GGCC	3 cut(s)	69, 544, 894
BspCNI	CTCAG	1 cut(s)	811
BspDI	ATCGAT	1 cut(s)	75
BspFNI	CGCG	1 cut(s)	175
BspLI	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
BspPI	GGATC	1 cut(s)	1470
BspT107I	GGYRCC	1 cut(s)	211
BspTNI	GGTCTC	1 cut(s)	1424
BsrDI	GCAATG	2 cut(s)	329, 552
BsrFI	RCCGGY	1 cut(s)	695
BsrI	ACTGG	1 cut(s)	70
BssAI	RCCGGY	1 cut(s)	695
BssMI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BssNAI	GTATAC	1 cut(s)	456
Bst1107I	GTATAC	1 cut(s)	456
Bst4CI	ACNGT	2 cut(s)	1066, 1181
Bst6I	CTCTTC	2 cut(s)	427, 731
BstBAI	YACGTR	1 cut(s)	58
BstC8I	GCNNGC	3 cut(s)	559, 752, 1012
BstDEI	CTNAG	1 cut(s)	819
BstF5I	GGATG	5 cut(s)	393, 423, 448, 858, 951
BstFNI	CGCG	1 cut(s)	175
BstH2I	RGCGCY	1 cut(s)	569
BstHHI	GCGC	1 cut(s)	568
BstKTI	GATC	5 cut(s)	79, 184, 1150, 1207, 1465
BstMAI	GTCTC	2 cut(s)	303, 1424
BstMBI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BstMWI	GCNNNNNNNGC	4 cut(s)	24, 153, 563, 1011
BstSCI	CCNGG	1 cut(s)	120
BstSFI	CTRYAG	1 cut(s)	86
BstSLI	GKGCMC	2 cut(s)	563, 1064
BstUI	CGCG	1 cut(s)	175
BstV1I	GCAGC	2 cut(s)	1302, 1528
BstV2I	GAAGAC	2 cut(s)	118, 387
BstXI	CCANNNNNNTGG	1 cut(s)	1513
BstZ17I	GTATAC	1 cut(s)	456
Bsu15I	ATCGAT	1 cut(s)	75
BsuRI	GGCC	3 cut(s)	69, 544, 894
BsuTUI	ATCGAT	1 cut(s)	75
BtgZI	GCGATG	1 cut(s)	1175
BtsCI	GGATG	5 cut(s)	393, 423, 448, 858, 951
BtsIMutI	CAGTG	2 cut(s)	1062, 1258
Cac8I	GCNNGC	3 cut(s)	559, 752, 1012
CfoI	GCGC	1 cut(s)	568
Cfr10I	RCCGGY	1 cut(s)	695
Cfr13I	GGNCC	2 cut(s)	232, 892
ClaI	ATCGAT	1 cut(s)	75
CseI	GACGC	3 cut(s)	121, 1040, 1349
Csp6I	GTAC	5 cut(s)	52, 158, 178, 979, 1017
CviAII	CATG	4 cut(s)	313, 805, 889, 1545
CviQI	GTAC	5 cut(s)	52, 158, 178, 979, 1017
DdeI	CTNAG	1 cut(s)	819
DpnI	GATC	5 cut(s)	78, 183, 1149, 1206, 1464
DpnII	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
DraI	TTTAAA	1 cut(s)	1402
DraIII	CACNNNGTG	1 cut(s)	775
DrdI	GACNNNNNNGTC	1 cut(s)	122
DseDI	GACNNNNNNGTC	1 cut(s)	122
Eam1104I	CTCTTC	2 cut(s)	427, 731
EarI	CTCTTC	2 cut(s)	427, 731
Eco147I	AGGCCT	1 cut(s)	544
Eco31I	GGTCTC	1 cut(s)	1424
Eco47I	GGWCC	1 cut(s)	232
Eco57I	CTGAAG	1 cut(s)	112
EcoT22I	ATGCAT	1 cut(s)	1350
FaeI	CATG	4 cut(s)	316, 808, 892, 1548
FatI	CATG	4 cut(s)	312, 804, 888, 1544
FbaI	TGATCA	2 cut(s)	1147, 1204
FblI	GTMKAC	2 cut(s)	135, 455
Fnu4HI	GCNGC	3 cut(s)	43, 1316, 1542
FokI	GGATG	5 cut(s)	400, 430, 455, 865, 958
Fsp4HI	GCNGC	3 cut(s)	43, 1316, 1542
FspBI	CTAG	2 cut(s)	942, 1371
GlaI	GCGC	1 cut(s)	567
GluI	GCNGC	3 cut(s)	43, 1316, 1542
HaeII	RGCGCY	1 cut(s)	569
HaeIII	GGCC	3 cut(s)	69, 544, 894
HapII	CCGG	5 cut(s)	37, 121, 440, 696, 914
HgaI	GACGC	3 cut(s)	121, 1040, 1349
HhaI	GCGC	1 cut(s)	568
Hin1II	CATG	4 cut(s)	316, 808, 892, 1548
Hin6I	GCGC	1 cut(s)	566
HinP1I	GCGC	1 cut(s)	566
HincII	GTYRAC	2 cut(s)	136, 424
HindII	GTYRAC	2 cut(s)	136, 424
HindIII	AAGCTT	2 cut(s)	299, 1139
HinfI	GANTC	3 cut(s)	364, 1272, 1510
HpaII	CCGG	5 cut(s)	37, 121, 440, 696, 914
HphI	GGTGA	4 cut(s)	14, 277, 1199, 1267
Hpy166II	GTNNAC	6 cut(s)	22, 52, 136, 424, 456, 561
Hpy188I	TCNGA	4 cut(s)	115, 1126, 1152, 1467
Hpy188III	TCNNGA	3 cut(s)	308, 1145, 1490
Hpy8I	GTNNAC	6 cut(s)	22, 52, 136, 424, 456, 561
Hpy99I	CGWCG	2 cut(s)	101, 137
HpyAV	CCTTC	5 cut(s)	211, 589, 628, 1109, 1261
HpyCH4III	ACNGT	2 cut(s)	1066, 1181
HpyCH4IV	ACGT	1 cut(s)	57
HpyCH4V	TGCA	7 cut(s)	270, 561, 585, 1005, 1348, 1366, 1544
HpyF10VI	GCNNNNNNNGC	4 cut(s)	24, 153, 563, 1011
HpyF3I	CTNAG	1 cut(s)	819
HpySE526I	ACGT	1 cut(s)	57
Hsp92II	CATG	4 cut(s)	316, 808, 892, 1548
HspAI	GCGC	1 cut(s)	566
Ksp22I	TGATCA	2 cut(s)	1147, 1204
Kzo9I	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
LmnI	GCTCC	3 cut(s)	39, 949, 1496
Lsp1109I	GCAGC	2 cut(s)	1302, 1528
LweI	GCATC	2 cut(s)	1357, 1528
MaeI	CTAG	2 cut(s)	942, 1371
MaeII	ACGT	1 cut(s)	57
MaeIII	GTNAC	5 cut(s)	763, 1052, 1078, 1337, 1393
MalI	GATC	5 cut(s)	78, 183, 1149, 1206, 1464
MboI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
MboII	GAAGA	8 cut(s)	118, 296, 359, 392, 444, 748, 844, 1237
MhlI	GDGCHC	3 cut(s)	195, 563, 1064
MlyI	GAGTC	1 cut(s)	1504
MmeI	TCCRAC	1 cut(s)	789
Mph1103I	ATGCAT	1 cut(s)	1350
MroXI	GAANNNNTTC	3 cut(s)	221, 408, 536
MseI	TTAA	7 cut(s)	528, 645, 906, 1109, 1308, 1401, 1518
MspI	CCGG	5 cut(s)	37, 121, 440, 696, 914
MspR9I	CCNGG	1 cut(s)	122
MvnI	CGCG	1 cut(s)	175
MwoI	GCNNNNNNNGC	4 cut(s)	24, 153, 563, 1011
NciI	CCSGG	1 cut(s)	122
NdeII	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
NlaIII	CATG	4 cut(s)	316, 808, 892, 1548
NlaIV	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
NmeAIII	GCCGAG	1 cut(s)	214
NmuCI	GTSAC	2 cut(s)	1052, 1337
NsiI	ATGCAT	1 cut(s)	1350
PceI	AGGCCT	1 cut(s)	544
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 186
PdmI	GAANNNNTTC	3 cut(s)	221, 408, 536
PfeI	GAWTC	2 cut(s)	364, 1272
PflFI	GACNNNGTC	1 cut(s)	617
PkrI	GCNGC	3 cut(s)	44, 1317, 1543
PleI	GAGTC	1 cut(s)	1504
PpsI	GAGTC	1 cut(s)	1504
Ppu21I	YACGTR	1 cut(s)	58
PsiI	TTATAA	3 cut(s)	666, 876, 1097
PspN4I	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
PspPI	GGNCC	2 cut(s)	232, 892
PsyI	GACNNNGTC	1 cut(s)	617
RsaI	GTAC	5 cut(s)	53, 159, 179, 980, 1018
RsaNI	GTAC	5 cut(s)	52, 158, 178, 979, 1017
SalI	GTCGAC	1 cut(s)	134
SaqAI	TTAA	7 cut(s)	528, 645, 906, 1109, 1308, 1401, 1518
SatI	GCNGC	3 cut(s)	43, 1316, 1542
Sau3AI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
Sau96I	GGNCC	2 cut(s)	232, 892
SchI	GAGTC	1 cut(s)	1504
ScrFI	CCNGG	1 cut(s)	122
SduI	GDGCHC	3 cut(s)	195, 563, 1064
SfaNI	GCATC	2 cut(s)	1357, 1528
SfcI	CTRYAG	1 cut(s)	86
SinI	GGWCC	1 cut(s)	232
SmlI	CTYRAG	2 cut(s)	247, 1319
SmoI	CTYRAG	2 cut(s)	247, 1319
SseBI	AGGCCT	1 cut(s)	544
SsiI	CCGC	4 cut(s)	25, 43, 145, 175
SspMI	CTAG	2 cut(s)	942, 1371
StuI	AGGCCT	1 cut(s)	544
StyD4I	CCNGG	1 cut(s)	120
TaaI	ACNGT	2 cut(s)	1066, 1181
TaiI	ACGT	1 cut(s)	60
TaqI	TCGA	4 cut(s)	75, 96, 135, 307
TatI	WGTACW	1 cut(s)	51
TauI	GCSGC	1 cut(s)	45
TfiI	GAWTC	2 cut(s)	364, 1272
Tru1I	TTAA	7 cut(s)	528, 645, 906, 1109, 1308, 1401, 1518
Tru9I	TTAA	7 cut(s)	528, 645, 906, 1109, 1308, 1401, 1518
TscAI	CASTG	2 cut(s)	1069, 1258
TseFI	GTSAC	2 cut(s)	1052, 1337
TseI	GCWGC	2 cut(s)	1315, 1541
Tsp45I	GTSAC	2 cut(s)	1052, 1337
TspDTI	ATGAA	8 cut(s)	139, 244, 401, 642, 749, 793, 845, 1281
TspRI	CASTG	2 cut(s)	1069, 1258
Tth111I	GACNNNGTC	1 cut(s)	617
VneI	GTGCAC	1 cut(s)	559
VpaK11BI	GGWCC	1 cut(s)	232
XapI	RAATTY	1 cut(s)	280
XmiI	GTMKAC	2 cut(s)	135, 455
XmnI	GAANNNNTTC	3 cut(s)	221, 408, 536
XspI	CTAG	2 cut(s)	942, 1371
Zsp2I	ATGCAT	1 cut(s)	1350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.