Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012815

Physical Location & Seq

Genomic Coordinates Forward (+)

7235212 .. 7238698

Gene Structure 3487 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1103300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1524 bp

ATGTATCGAAGTCTTGGCGTTCACCGCCTCATATACAGGAGCCGCATAGCGTACTACGTGAAAACTGGCCTCATCGATCAAGCACTCCAGGTGTTCGACGAAATGACCCACTCGGACTGCCGCGTTTTCAGCGTCGACTACAACCGCTTCATCGGCGTACTCGTCAAGCACTCGCGTTACGATCTCGCCGAGCACTACTACTACGAAATGGTGCCGAAGGGATTCTCATTGGACCCCTTTACTTACTCCAGGTTCATTTCTGGCCTGTGTAAGATTAGAAATTTCACCCTCATTGAGAAGCTTCTTCGAGACATGGATAGGATTGGGGCATTGCCTGACATCTGGGCTTTCAATATATATTTGAATCTTCTGTGCCAAGAAAACGGGACGGATTTTGCTTTGGAATTGTTTCATAGGATGGTTGATAAAGGAAGAGAGCCGGATGTTGTATCGTATACTATAATTATCGATGGGTTGTGTAAAGCTGGACAATTTGATATGGCAGTTGATATTTGGAATGGTATGATTAGGAAAGGGTTTAGGCCGGATAACATTGCGTGCACAGCGCTGGTTGTTGGGTTGTGCAAGGGTGGGAAGGTTGATTTGGCTTATGACCTTGTCATTGATGAAATGAAGGGTAGAATCAATTTCAGTAGTTTGATTTATAATGCATTGATTAGCGGGTTTTGTCGTGCTGGTAGGATTGATAAGGCACAGGCAATCAAGTCATTTATGAAGAGAAATGGGTGCGAGCCGGATTTGGTTTCTCACAATGTATTGTTGAATTATTGCTGTAATGCGTTCATGTTGGAGGAGGCTGAGAAGTTGATGAAGAAAATGGAGAGGGGTGGGATGGAACCGGATGTGTATAGTTATAATCAGCTTCTCATGGGCCTTTGTAAAGCTAATAGACCGGATAAGGCATATTTGTTGATGCGAACTAGGATGGAGCCAAAAGGGTTGTGTAATGTTGTGTCATATAATACCATCATTACAGCATTTTGCAAGGCAAGCCGTACTGGAAGTGCTTACAAACTGTTTGAGGAAATGCGTCACAAGGGCACTGTGCCAGATTTGGTTACATTCACTATTCTTATAAATGCCTTTTTAAGAGAAGGTAGTTCAGATATAGTCAAGAAGCTTCTTGATCAGATGACAGCAATGGGTCTGTTGCCTGACCGTATATTTTACACCACAATAGTTGATCACTTATGTAAGAGTGGGAATATTGAAATGGCTTGTAGTGTTTTCAGTGAAATGTTAGAGAAGGGAATCACCCCTGATGTGATTTCATATAATGCTCTCATTAACGGGCTGCTCAAGGCTTCTAAAGTGAGTGACGCTATGCATCTTTATGAGGAAATGCAGACTAGAGGATGTAGCGCTGACGAGGTAACTTTTAAATTGATAATTGGGGGTCTCATAAGGGAGAATAAGGTTTCAGTCGCTTGTAGGGTATGGGATCAGATGATGGAAAAGGGCTTTACTCTTGATGGAGCTTTCTCCGAGACTCTGAGTCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016020 GO:0016070 GO:0016787 GO:0016788 GO:0031090 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:0098588 GO:0098805 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_2 PPR_3

Protein Analysis

508

Amino Acids

57.58

Weight (kDa)

7.78

Isoelectric Point (pI)

26.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	47 - 91	4.7e-07	PPR repeat family
PPR_2	PF13041	112 - 161	3.9e-14	PPR repeat family
PPR_1	PF12854	143 - 175	5.9e-10	PPR repeat
PPR_2	PF13041	147 - 196	1.1e-14	PPR repeat family
PPR	PF01535	150 - 180	4e-08	PPR repeat
PPR_3	PF13812	209 - 266	9.7e-08	Pentatricopeptide repeat domain
PPR_2	PF13041	220 - 266	1.8e-13	PPR repeat family
PPR	PF01535	221 - 250	1.6e-06	PPR repeat
PPR_1	PF12854	221 - 245	1.5e-07	PPR repeat
PPR_1	PF12854	248 - 281	3e-06	PPR repeat
PPR_2	PF13041	252 - 301	5.8e-14	PPR repeat family
PPR_1	PF12854	284 - 313	9.2e-08	PPR repeat
PPR_1	PF12854	320 - 351	3.7e-10	PPR repeat
PPR_3	PF13812	322 - 369	4.6e-07	Pentatricopeptide repeat domain
PPR_2	PF13041	323 - 370	3.7e-17	PPR repeat family
PPR	PF01535	325 - 354	5.1e-07	PPR repeat
TPR_24	PF23276	361 - 455	4.1e-06	Fungal tetratrico peptide repeats
PPR_long	PF17177	378 - 465	6.2e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	380 - 437	6e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	388 - 420	2.4e-07	PPR repeat
PPR_2	PF13041	392 - 439	4.6e-15	PPR repeat family
PPR	PF01535	397 - 425	1.8e-06	PPR repeat
PPR_1	PF12854	423 - 456	2.7e-08	PPR repeat
PPR_2	PF13041	427 - 475	6.2e-15	PPR repeat family
PPR	PF01535	430 - 460	4e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G13040 AT1G13040
fragaria_vesca	FvH4_4g25420
malus_domestica	MD13G1103100.v1.1 MD16G1103300.v1.1
prunus_persica	Prupe.1G242700_v2.0.a1
pyrus_communis	pycom13g09010 pycom16g08810
rosa_chinensis	RchiOBHm_Chr4g0432731
rosa_laevigata	RLG00000006817
rosa_multiflora	Rmu_co8522421.1_g000001
rosa_roxburghii	Rroxscaffold_5G00374190
rosa_rugosa	Rorug04G0262000
rosa_samantha	Rh4AG316500 Rh4BG324500 Rh4CG339500 Rh4DG319800
rosa_wichuraiana	Rw4G027530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	666, 876, 1097
AccB1I	GGYRCC	1 cut(s)	211
AccI	GTMKAC	2 cut(s)	135, 455
AccII	CGCG	2 cut(s)	123, 175
AciI	CCGC	5 cut(s)	25, 43, 121, 145, 681
AclWI	GGATC	1 cut(s)	1470
AcsI	RAATTY	1 cut(s)	280
AfaI	GTAC	3 cut(s)	53, 159, 1018
AfeI	AGCGCT	2 cut(s)	567, 1384
AgsI	TTSAA	4 cut(s)	352, 364, 784, 1232
AhdI	GACNNNNNGTC	1 cut(s)	1515
AjnI	CCWGG	2 cut(s)	87, 248
AjuI	GAANNNNNNNTTGG	2 cut(s)	587, 619
AluBI	AGCT	6 cut(s)	301, 485, 883, 905, 1141, 1499
AluI	AGCT	6 cut(s)	301, 485, 883, 905, 1141, 1499
Alw21I	GWGCWC	2 cut(s)	195, 563
Alw26I	GTCTC	3 cut(s)	303, 1424, 1502
Alw44I	GTGCAC	1 cut(s)	559
AlwI	GGATC	1 cut(s)	1470
Aor51HI	AGCGCT	2 cut(s)	567, 1384
AoxI	GGCC	4 cut(s)	67, 262, 542, 892
ApaLI	GTGCAC	1 cut(s)	559
ApeKI	GCWGC	1 cut(s)	1315
ApoI	RAATTY	1 cut(s)	280
Asp700I	GAANNNNTTC	2 cut(s)	221, 408
AspLEI	GCGC	2 cut(s)	568, 1385
AspS9I	GGNCC	2 cut(s)	232, 892
AsuHPI	GGTGA	3 cut(s)	14, 277, 1267
AvaII	GGWCC	1 cut(s)	232
BaeGI	GKGCMC	2 cut(s)	563, 1064
BanI	GGYRCC	1 cut(s)	211
Bbv12I	GWGCWC	2 cut(s)	195, 563
BbvI	GCAGC	1 cut(s)	1302
BccI	CCATC	7 cut(s)	412, 464, 847, 940, 995, 1465, 1487
BceAI	ACGGC	1 cut(s)	999
BciT130I	CCWGG	2 cut(s)	89, 250
BclI	TGATCA	2 cut(s)	1147, 1204
BcoDI	GTCTC	3 cut(s)	303, 1424, 1502
BfaI	CTAG	2 cut(s)	942, 1371
BfoI	RGCGCY	2 cut(s)	569, 1386
BisI	GCNGC	3 cut(s)	43, 121, 1316
BlsI	GCNGC	3 cut(s)	44, 122, 1317
Bme1390I	CCNGG	2 cut(s)	89, 250
Bme18I	GGWCC	1 cut(s)	232
BmeRI	GACNNNNNGTC	1 cut(s)	1515
BmgT120I	GGNCC	2 cut(s)	232, 892
BmiI	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
BmrFI	CCNGG	2 cut(s)	89, 250
BmsI	GCATC	2 cut(s)	924, 1357
BpmI	CTGGAG	2 cut(s)	71, 232
BpuEI	CTTGAG	1 cut(s)	1304
Bsa29I	ATCGAT	2 cut(s)	75, 468
BsaAI	YACGTR	1 cut(s)	58
BsaI	GGTCTC	1 cut(s)	1424
BsaWI	WCCGGW	2 cut(s)	859, 913
Bse1I	ACTGG	2 cut(s)	70, 1024
Bse3DI	GCAATG	3 cut(s)	329, 552, 1167
BseBI	CCWGG	2 cut(s)	89, 250
BseCI	ATCGAT	2 cut(s)	75, 468
BseGI	GGATG	6 cut(s)	423, 448, 858, 868, 951, 1382
BseMI	GCAATG	3 cut(s)	329, 552, 1167
BseMII	CTCAG	2 cut(s)	810, 1505
BseNI	ACTGG	2 cut(s)	70, 1024
BseRI	GAGGAG	1 cut(s)	827
BseSI	GKGCMC	2 cut(s)	563, 1064
BseXI	GCAGC	1 cut(s)	1302
Bsh1236I	CGCG	2 cut(s)	123, 175
BshFI	GGCC	4 cut(s)	69, 264, 544, 894
BshNI	GGYRCC	1 cut(s)	211
BshVI	ATCGAT	2 cut(s)	75, 468
BsiHKAI	GWGCWC	2 cut(s)	195, 563
BsiSI	CCGG	5 cut(s)	440, 545, 755, 860, 914
BslFI	GGGAC	1 cut(s)	400
BsmAI	GTCTC	3 cut(s)	303, 1424, 1502
BsmFI	GGGAC	1 cut(s)	400
BsnI	GGCC	4 cut(s)	69, 264, 544, 894
Bso31I	GGTCTC	1 cut(s)	1424
Bsp1286I	GDGCHC	3 cut(s)	195, 563, 1064
Bsp143I	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BspACI	CCGC	5 cut(s)	25, 43, 121, 145, 681
BspANI	GGCC	4 cut(s)	69, 264, 544, 894
BspCNI	CTCAG	2 cut(s)	811, 1506
BspDI	ATCGAT	2 cut(s)	75, 468
BspFNI	CGCG	2 cut(s)	123, 175
BspLI	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
BspPI	GGATC	1 cut(s)	1470
BspT107I	GGYRCC	1 cut(s)	211
BspTNI	GGTCTC	1 cut(s)	1424
BsrDI	GCAATG	3 cut(s)	329, 552, 1167
BsrI	ACTGG	2 cut(s)	70, 1024
BssMI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BssNAI	GTATAC	1 cut(s)	456
Bst1107I	GTATAC	1 cut(s)	456
Bst2UI	CCWGG	2 cut(s)	89, 250
Bst4CI	ACNGT	3 cut(s)	1038, 1066, 1181
Bst6I	CTCTTC	2 cut(s)	427, 731
BstBAI	YACGTR	1 cut(s)	58
BstC8I	GCNNGC	3 cut(s)	559, 752, 1012
BstDEI	CTNAG	2 cut(s)	819, 1514
BstF5I	GGATG	6 cut(s)	423, 448, 858, 868, 951, 1382
BstFNI	CGCG	2 cut(s)	123, 175
BstH2I	RGCGCY	2 cut(s)	569, 1386
BstHHI	GCGC	2 cut(s)	568, 1385
BstKTI	GATC	5 cut(s)	79, 184, 1150, 1207, 1465
BstMAI	GTCTC	3 cut(s)	303, 1424, 1502
BstMBI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
BstMWI	GCNNNNNNNGC	5 cut(s)	24, 129, 153, 563, 1011
BstNI	CCWGG	2 cut(s)	89, 250
BstSCI	CCNGG	2 cut(s)	87, 248
BstSLI	GKGCMC	2 cut(s)	563, 1064
BstUI	CGCG	2 cut(s)	123, 175
BstV1I	GCAGC	1 cut(s)	1302
BstZ17I	GTATAC	1 cut(s)	456
Bsu15I	ATCGAT	2 cut(s)	75, 468
BsuRI	GGCC	4 cut(s)	69, 264, 544, 894
BsuTUI	ATCGAT	2 cut(s)	75, 468
BtsCI	GGATG	6 cut(s)	423, 448, 858, 868, 951, 1382
BtsIMutI	CAGTG	2 cut(s)	1062, 1258
Cac8I	GCNNGC	3 cut(s)	559, 752, 1012
CfoI	GCGC	2 cut(s)	568, 1385
Cfr13I	GGNCC	2 cut(s)	232, 892
ClaI	ATCGAT	2 cut(s)	75, 468
CseI	GACGC	3 cut(s)	121, 1040, 1349
Csp6I	GTAC	3 cut(s)	52, 158, 1017
CspCI	CAANNNNNGTGG	2 cut(s)	1183, 1218
CviAII	CATG	3 cut(s)	313, 805, 889
CviQI	GTAC	3 cut(s)	52, 158, 1017
DdeI	CTNAG	2 cut(s)	819, 1514
DpnI	GATC	5 cut(s)	78, 183, 1149, 1206, 1464
DpnII	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
DraI	TTTAAA	1 cut(s)	1402
DriI	GACNNNNNGTC	1 cut(s)	1515
Eam1104I	CTCTTC	2 cut(s)	427, 731
Eam1105I	GACNNNNNGTC	1 cut(s)	1515
EarI	CTCTTC	2 cut(s)	427, 731
Eco31I	GGTCTC	1 cut(s)	1424
Eco47I	GGWCC	1 cut(s)	232
Eco47III	AGCGCT	2 cut(s)	567, 1384
EcoRII	CCWGG	2 cut(s)	87, 248
EcoT22I	ATGCAT	2 cut(s)	673, 1350
FaeI	CATG	3 cut(s)	316, 808, 892
FaqI	GGGAC	1 cut(s)	400
FatI	CATG	3 cut(s)	312, 804, 888
FauI	CCCGC	1 cut(s)	674
FbaI	TGATCA	2 cut(s)	1147, 1204
FblI	GTMKAC	2 cut(s)	135, 455
Fnu4HI	GCNGC	3 cut(s)	43, 121, 1316
FokI	GGATG	6 cut(s)	430, 455, 865, 875, 958, 1389
Fsp4HI	GCNGC	3 cut(s)	43, 121, 1316
FspBI	CTAG	2 cut(s)	942, 1371
GlaI	GCGC	2 cut(s)	567, 1384
GluI	GCNGC	3 cut(s)	43, 121, 1316
GsuI	CTGGAG	2 cut(s)	71, 232
HaeII	RGCGCY	2 cut(s)	569, 1386
HaeIII	GGCC	4 cut(s)	69, 264, 544, 894
HapII	CCGG	5 cut(s)	440, 545, 755, 860, 914
HgaI	GACGC	3 cut(s)	121, 1040, 1349
HhaI	GCGC	2 cut(s)	568, 1385
Hin1II	CATG	3 cut(s)	316, 808, 892
Hin6I	GCGC	2 cut(s)	566, 1383
HinP1I	GCGC	2 cut(s)	566, 1383
HincII	GTYRAC	1 cut(s)	136
HindII	GTYRAC	1 cut(s)	136
HindIII	AAGCTT	2 cut(s)	299, 1139
HinfI	GANTC	6 cut(s)	222, 364, 642, 1272, 1510, 1516
HpaII	CCGG	5 cut(s)	440, 545, 755, 860, 914
HphI	GGTGA	3 cut(s)	14, 277, 1267
Hpy166II	GTNNAC	4 cut(s)	22, 136, 456, 561
Hpy188I	TCNGA	6 cut(s)	115, 1126, 1152, 1467, 1507, 1515
Hpy188III	TCNNGA	4 cut(s)	308, 1135, 1145, 1490
Hpy8I	GTNNAC	4 cut(s)	22, 136, 456, 561
Hpy99I	CGWCG	2 cut(s)	101, 137
HpyAV	CCTTC	5 cut(s)	211, 589, 628, 1109, 1261
HpyCH4III	ACNGT	3 cut(s)	1038, 1066, 1181
HpyCH4IV	ACGT	1 cut(s)	57
HpyCH4V	TGCA	6 cut(s)	561, 585, 671, 1005, 1348, 1366
HpyF10VI	GCNNNNNNNGC	5 cut(s)	24, 129, 153, 563, 1011
HpyF3I	CTNAG	2 cut(s)	819, 1514
HpySE526I	ACGT	1 cut(s)	57
Hsp92II	CATG	3 cut(s)	316, 808, 892
HspAI	GCGC	2 cut(s)	566, 1383
Ksp22I	TGATCA	2 cut(s)	1147, 1204
Kzo9I	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
LmnI	GCTCC	3 cut(s)	39, 949, 1496
Lsp1109I	GCAGC	1 cut(s)	1302
LweI	GCATC	2 cut(s)	924, 1357
MaeI	CTAG	2 cut(s)	942, 1371
MaeII	ACGT	1 cut(s)	57
MaeIII	GTNAC	5 cut(s)	176, 1052, 1078, 1337, 1393
MalI	GATC	5 cut(s)	78, 183, 1149, 1206, 1464
MboI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
MboII	GAAGA	5 cut(s)	296, 359, 444, 748, 844
MhlI	GDGCHC	3 cut(s)	195, 563, 1064
MluCI	AATT	8 cut(s)	280, 404, 462, 491, 646, 784, 1403, 1410
MlyI	GAGTC	1 cut(s)	1504
MmeI	TCCRAC	1 cut(s)	789
Mph1103I	ATGCAT	2 cut(s)	673, 1350
MroXI	GAANNNNTTC	2 cut(s)	221, 408
MseI	TTAA	3 cut(s)	1109, 1308, 1401
MslI	CAYNNNNRTG	1 cut(s)	1353
MspI	CCGG	5 cut(s)	440, 545, 755, 860, 914
MspR9I	CCNGG	2 cut(s)	89, 250
MvaI	CCWGG	2 cut(s)	89, 250
MvnI	CGCG	2 cut(s)	123, 175
MwoI	GCNNNNNNNGC	5 cut(s)	24, 129, 153, 563, 1011
NdeII	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
NlaIII	CATG	3 cut(s)	316, 808, 892
NlaIV	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
NmeAIII	GCCGAG	1 cut(s)	214
NmuCI	GTSAC	2 cut(s)	1052, 1337
NsiI	ATGCAT	2 cut(s)	673, 1350
PcsI	WCGNNNNNNNCGW	2 cut(s)	159, 186
PdmI	GAANNNNTTC	2 cut(s)	221, 408
PfeI	GAWTC	4 cut(s)	222, 364, 642, 1272
PflFI	GACNNNGTC	1 cut(s)	617
PkrI	GCNGC	3 cut(s)	44, 122, 1317
PleI	GAGTC	1 cut(s)	1504
PpsI	GAGTC	1 cut(s)	1504
Ppu21I	YACGTR	1 cut(s)	58
PsiI	TTATAA	3 cut(s)	666, 876, 1097
Psp6I	CCWGG	2 cut(s)	87, 248
PspGI	CCWGG	2 cut(s)	87, 248
PspN4I	GGNNCC	5 cut(s)	41, 213, 234, 858, 951
PspPI	GGNCC	2 cut(s)	232, 892
PsyI	GACNNNGTC	1 cut(s)	617
RsaI	GTAC	3 cut(s)	53, 159, 1018
RsaNI	GTAC	3 cut(s)	52, 158, 1017
RseI	CAYNNNNRTG	1 cut(s)	1353
SalI	GTCGAC	1 cut(s)	134
SaqAI	TTAA	3 cut(s)	1109, 1308, 1401
SatI	GCNGC	3 cut(s)	43, 121, 1316
Sau3AI	GATC	5 cut(s)	76, 181, 1147, 1204, 1462
Sau96I	GGNCC	2 cut(s)	232, 892
SchI	GAGTC	1 cut(s)	1504
ScrFI	CCNGG	2 cut(s)	89, 250
SduI	GDGCHC	3 cut(s)	195, 563, 1064
SfaNI	GCATC	2 cut(s)	924, 1357
SinI	GGWCC	1 cut(s)	232
SmiMI	CAYNNNNRTG	1 cut(s)	1353
SmlI	CTYRAG	1 cut(s)	1319
SmoI	CTYRAG	1 cut(s)	1319
Sse9I	AATT	8 cut(s)	280, 404, 462, 491, 646, 784, 1403, 1410
SsiI	CCGC	5 cut(s)	25, 43, 121, 145, 681
SspI	AATATT	1 cut(s)	1228
SspMI	CTAG	2 cut(s)	942, 1371
StyD4I	CCNGG	2 cut(s)	87, 248
TaaI	ACNGT	3 cut(s)	1038, 1066, 1181
TaiI	ACGT	1 cut(s)	60
TaqI	TCGA	6 cut(s)	7, 75, 96, 135, 307, 468
TasI	AATT	8 cut(s)	280, 404, 462, 491, 646, 784, 1403, 1410
TauI	GCSGC	2 cut(s)	45, 123
TfiI	GAWTC	4 cut(s)	222, 364, 642, 1272
Tru1I	TTAA	3 cut(s)	1109, 1308, 1401
Tru9I	TTAA	3 cut(s)	1109, 1308, 1401
TscAI	CASTG	2 cut(s)	1069, 1258
TseFI	GTSAC	2 cut(s)	1052, 1337
TseI	GCWGC	1 cut(s)	1315
Tsp45I	GTSAC	2 cut(s)	1052, 1337
TspDTI	ATGAA	9 cut(s)	139, 244, 401, 642, 647, 749, 793, 845, 1281
TspGWI	ACGGA	1 cut(s)	404
TspRI	CASTG	2 cut(s)	1069, 1258
Tth111I	GACNNNGTC	1 cut(s)	617
VneI	GTGCAC	1 cut(s)	559
VpaK11BI	GGWCC	1 cut(s)	232
XapI	RAATTY	1 cut(s)	280
XmiI	GTMKAC	2 cut(s)	135, 455
XmnI	GAANNNNTTC	2 cut(s)	221, 408
XspI	CTAG	2 cut(s)	942, 1371
Zsp2I	ATGCAT	2 cut(s)	673, 1350

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.