FvH4_4g25900

synapsis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008767
Physical Location & Seq
Reverse (-)
27527865 .. 27530973
3109 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g25900.t3

Sequence Viewer

Length: 1002 bp
ATGGCGGGCACTCTGGCTCTGCTACCAAGCAATCCTCCGATGACTTCATCGTCTCTGAGCTCCATCCGAGAGCGCTGGGAGATCGAGTTCTCTCGCTTCTTCGGCTACCCTCCGCTCACCTCCACGTGTCCAGATCTCGTCCCTCTCCCAATGAAGATCAGGAATCGACGGTCGGGTGGTAACTGGATCTCCGCCGCCTCCGCCGCCATGCTTCAGCTCATCTACGACCACTCCAGCTCTGACGTCATCCTCACCGTTTGCTGCAGCGGAAAGATCCAGGAGGAGCACTACGTATCGAAGCTACTCTTTGCCTGGCCTCAGGTGGCATGTATGTCTGGATTTCCTGCTAGGGGCAGTAGAGCTGTCTTTGCCAGCTACAGAGATTCTTTGGGCCAGATCCAGAAATTTGCTTTTCGATTTTTGTCTGTCAATGAAGCAGAGAAGTTCATGACTCTTATGAAGGAGATTTTCAAGGAAGTGAGGGACATTCAACCTCTTACTGCTGACTATGGATCGGAAATTTCATCACATTCTGAGTTCATGTCTTCTGATAGACCTTTAAACAGGCAAAACCATTCTTCTCTCTGTTTCAGAACTTCCAAGGACTTGGTTGTCATGACTCCTGGTCACACTCATTCTCCTCAAATATCACCAAGCTTAATGAAGGCTTATACTCCACAAACCTCACCTGGCTTAATGAAGGATTATTCACCTCAAATGCCACCAAGCTGGACTCATGAAGCAGCTCAATACAGACAAACTCAGCACATGTCCCCTATTCAGAACGTTCAGAGCAATTTTGCTGCCTTGCCTCCCAGTTTCACCTCATTAATGTCTAACTGTCAACCTGTGGTAGAACAAGCTCCAGCCCAATCAACTGCATCCAAGGAAGTTGACCTCAAGGCCCTAATTGCGAGATGTATGGAAGACTCTTCCTTCCAAGATATATTGAACCAAGTCGAGATAGTTATGAGTGAAATGGGGGCTGATTTGAGGCTGTAG

Protein Analysis

334

Amino Acids

37.14

Weight (kDa)

6.71

Isoelectric Point (pI)

63.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH_PHS1 PF25349 24 - 166 8.6e-63 Poor homologous synapsis protein PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 49, 611
AatII GACGTC 1 cut(s) 246
AccBSI CCGCTC 1 cut(s) 115
AciI CCGC 7 cut(s) 5, 113, 192, 195, 201, 204, 267
AclI AACGTT 1 cut(s) 786
AclWI GGATC 4 cut(s) 194, 268, 391, 520
AcsI RAATTY 2 cut(s) 404, 519
AcuI CTGAAG 1 cut(s) 197
AcvI CACGTG 1 cut(s) 126
AcyI GRCGYC 1 cut(s) 243
AfeI AGCGCT 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 350
AflIII ACRYGT 2 cut(s) 125, 768
AgsI TTSAA 3 cut(s) 472, 491, 952
AhdI GACNNNNNGTC 1 cut(s) 624
AjnI CCWGG 4 cut(s) 276, 311, 622, 688
AloI GAACNNNNNNTCC 2 cut(s) 71, 103
Alw21I GWGCWC 2 cut(s) 62, 288
Alw26I GTCTC 1 cut(s) 57
AlwI GGATC 4 cut(s) 194, 268, 391, 520
Aor51HI AGCGCT 1 cut(s) 74
AoxI GGCC 3 cut(s) 314, 391, 903
ApeKI GCWGC 4 cut(s) 261, 264, 743, 803
ApoI RAATTY 2 cut(s) 404, 519
AseI ATTAAT 1 cut(s) 830
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 391, 904
AsuHPI GGTGA 6 cut(s) 109, 244, 642, 678, 702, 814
AxyI CCTNAGG 1 cut(s) 318
BaeGI GKGCMC 1 cut(s) 11
BanII GRGCYC 1 cut(s) 62
BbrPI CACGTG 1 cut(s) 126
BbsI GAAGAC 2 cut(s) 537, 933
Bbv12I GWGCWC 2 cut(s) 62, 288
BbvI GCAGC 4 cut(s) 248, 276, 755, 790
BccI CCATC 1 cut(s) 71
BciT130I CCWGG 4 cut(s) 278, 313, 624, 690
BcoDI GTCTC 1 cut(s) 57
BfaI CTAG 1 cut(s) 348
BfmI CTRYAG 3 cut(s) 262, 376, 998
BfoI RGCGCY 1 cut(s) 76
BglII AGATCT 1 cut(s) 133
BisI GCNGC 6 cut(s) 195, 204, 262, 265, 744, 804
BlsI GCNGC 6 cut(s) 196, 205, 263, 266, 745, 805
Bme1390I CCNGG 4 cut(s) 278, 313, 624, 690
BmeRI GACNNNNNGTC 1 cut(s) 624
BmgT120I GGNCC 2 cut(s) 391, 904
BmrFI CCNGG 4 cut(s) 278, 313, 624, 690
BmrI ACTGGG 1 cut(s) 810
BmsI GCATC 1 cut(s) 890
BmuI ACTGGG 1 cut(s) 810
BpiI GAAGAC 2 cut(s) 537, 933
BpmI CTGGAG 2 cut(s) 217, 849
BpuEI CTTGAG 1 cut(s) 884
BsaAI YACGTR 2 cut(s) 126, 292
BsaHI GRCGYC 1 cut(s) 243
BsaJI CCNNGG 2 cut(s) 600, 885
BsaXI ACNNNNNCTCC 8 cut(s) 71, 101, 173, 203, 215, 245, 622, 652
Bsc4I CCNNNNNNNGG 1 cut(s) 350
Bse1I ACTGG 2 cut(s) 188, 816
Bse21I CCTNAGG 1 cut(s) 318
BseBI CCWGG 4 cut(s) 278, 313, 624, 690
BseDI CCNNGG 2 cut(s) 600, 885
BseGI GGATG 3 cut(s) 63, 246, 881
BseLI CCNNNNNNNGG 1 cut(s) 350
BseMII CTCAG 4 cut(s) 47, 332, 525, 776
BseNI ACTGG 2 cut(s) 188, 816
BseRI GAGGAG 2 cut(s) 296, 630
BseSI GKGCMC 1 cut(s) 11
BseXI GCAGC 4 cut(s) 248, 276, 755, 790
BseYI CCCAGC 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 3 cut(s) 316, 393, 905
BsiEI CGRYCG 1 cut(s) 173
BsiHKAI GWGCWC 2 cut(s) 62, 288
BslFI GGGAC 3 cut(s) 125, 497, 757
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 1 cut(s) 57
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 3 cut(s) 125, 497, 757
BsnI GGCC 3 cut(s) 316, 393, 905
Bsp1286I GDGCHC 3 cut(s) 11, 62, 288
Bsp143I GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
BspACI CCGC 7 cut(s) 5, 113, 192, 195, 201, 204, 267
BspANI GGCC 3 cut(s) 316, 393, 905
BspCNI CTCAG 4 cut(s) 48, 331, 526, 775
BspHI TCATGA 3 cut(s) 447, 615, 736
BspMAI CTGCAG 1 cut(s) 266
BspPI GGATC 4 cut(s) 194, 268, 391, 520
BsrBI CCGCTC 1 cut(s) 115
BsrI ACTGG 2 cut(s) 188, 816
BssECI CCNNGG 2 cut(s) 600, 885
BssMI GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
BssNI GRCGYC 1 cut(s) 243
BssT1I CCWWGG 2 cut(s) 600, 885
Bst2UI CCWGG 4 cut(s) 278, 313, 624, 690
Bst4CI ACNGT 3 cut(s) 171, 256, 842
Bst6I CTCTTC 1 cut(s) 937
BstACI GRCGYC 1 cut(s) 243
BstBAI YACGTR 2 cut(s) 126, 292
BstC8I GCNNGC 2 cut(s) 7, 373
BstDEI CTNAG 4 cut(s) 56, 318, 534, 762
BstF5I GGATG 3 cut(s) 63, 246, 881
BstH2I RGCGCY 1 cut(s) 76
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 7 cut(s) 84, 136, 159, 189, 276, 399, 515
BstMAI GTCTC 1 cut(s) 57
BstMBI GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 5 cut(s) 102, 200, 203, 368, 911
BstNI CCWGG 4 cut(s) 278, 313, 624, 690
BstNSI RCATGY 2 cut(s) 330, 772
BstSCI CCNGG 4 cut(s) 276, 311, 622, 688
BstSFI CTRYAG 3 cut(s) 262, 376, 998
BstSLI GKGCMC 1 cut(s) 11
BstSNI TACGTA 1 cut(s) 292
BstV1I GCAGC 4 cut(s) 248, 276, 755, 790
BstV2I GAAGAC 2 cut(s) 537, 933
BstX2I RGATCY 4 cut(s) 133, 186, 273, 396
BstXI CCANNNNNNTGG 2 cut(s) 607, 729
BstYI RGATCY 4 cut(s) 133, 186, 273, 396
Bsu36I CCTNAGG 1 cut(s) 318
BsuRI GGCC 3 cut(s) 316, 393, 905
BtsCI GGATG 3 cut(s) 63, 246, 881
Cac8I GCNNGC 2 cut(s) 7, 373
CciI TCATGA 3 cut(s) 447, 615, 736
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 391, 904
CviAII CATG 7 cut(s) 208, 327, 448, 541, 616, 737, 769
DdeI CTNAG 4 cut(s) 56, 318, 534, 762
DpnI GATC 7 cut(s) 83, 135, 158, 188, 275, 398, 514
DpnII GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
DraI TTTAAA 1 cut(s) 561
DrdI GACNNNNNNGTC 2 cut(s) 49, 611
DriI GACNNNNNGTC 1 cut(s) 624
DseDI GACNNNNNNGTC 2 cut(s) 49, 611
Eam1104I CTCTTC 1 cut(s) 937
Eam1105I GACNNNNNGTC 1 cut(s) 624
EarI CTCTTC 1 cut(s) 937
EciI GGCGGA 2 cut(s) 181, 190
Ecl136II GAGCTC 1 cut(s) 60
Eco105I TACGTA 1 cut(s) 292
Eco130I CCWWGG 2 cut(s) 600, 885
Eco24I GRGCYC 1 cut(s) 62
Eco47III AGCGCT 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 60
Eco57I CTGAAG 1 cut(s) 197
Eco72I CACGTG 1 cut(s) 126
Eco81I CCTNAGG 1 cut(s) 318
EcoICRI GAGCTC 1 cut(s) 60
EcoO109I RGGNCCY 1 cut(s) 904
EcoRII CCWGG 4 cut(s) 276, 311, 622, 688
EcoT14I CCWWGG 2 cut(s) 600, 885
EcoT38I GRGCYC 1 cut(s) 62
ErhI CCWWGG 2 cut(s) 600, 885
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 7 cut(s) 211, 330, 451, 544, 619, 740, 772
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 3 cut(s) 125, 497, 757
FatI CATG 7 cut(s) 207, 326, 447, 540, 615, 736, 768
Fnu4HI GCNGC 6 cut(s) 195, 204, 262, 265, 744, 804
FokI GGATG 3 cut(s) 50, 233, 868
FriOI GRGCYC 1 cut(s) 62
Fsp4HI GCNGC 6 cut(s) 195, 204, 262, 265, 744, 804
FspBI CTAG 1 cut(s) 348
GlaI GCGC 1 cut(s) 74
GluI GCNGC 6 cut(s) 195, 204, 262, 265, 744, 804
GsaI CCCAGC 1 cut(s) 79
GsuI CTGGAG 2 cut(s) 217, 849
HaeII RGCGCY 1 cut(s) 76
HaeIII GGCC 3 cut(s) 316, 393, 905
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 243
Hin1II CATG 7 cut(s) 211, 330, 451, 544, 619, 740, 772
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HincII GTYRAC 2 cut(s) 845, 895
HindII GTYRAC 2 cut(s) 845, 895
HindIII AAGCTT 1 cut(s) 655
HinfI GANTC 6 cut(s) 163, 383, 451, 619, 733, 929
HphI GGTGA 6 cut(s) 109, 244, 642, 678, 702, 814
Hpy166II GTNNAC 2 cut(s) 845, 895
Hpy188III TCNNGA 8 cut(s) 131, 160, 336, 400, 448, 616, 737, 961
Hpy8I GTNNAC 2 cut(s) 845, 895
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 4 cut(s) 454, 658, 694, 946
HpyCH4III ACNGT 3 cut(s) 171, 256, 842
HpyCH4IV ACGT 4 cut(s) 125, 243, 291, 786
HpyCH4V TGCA 2 cut(s) 264, 881
HpyF10VI GCNNNNNNNGC 5 cut(s) 102, 200, 203, 368, 911
HpyF3I CTNAG 4 cut(s) 56, 318, 534, 762
HpySE526I ACGT 4 cut(s) 125, 243, 291, 786
Hsp92I GRCGYC 1 cut(s) 243
Hsp92II CATG 7 cut(s) 211, 330, 451, 544, 619, 740, 772
HspAI GCGC 1 cut(s) 73
Kzo9I GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
LmnI GCTCC 3 cut(s) 65, 283, 868
Lsp1109I GCAGC 4 cut(s) 248, 276, 755, 790
LweI GCATC 1 cut(s) 890
MaeI CTAG 1 cut(s) 348
MaeII ACGT 4 cut(s) 125, 243, 291, 786
MaeIII GTNAC 2 cut(s) 179, 626
MalI GATC 7 cut(s) 83, 135, 158, 188, 275, 398, 514
MbiI CCGCTC 1 cut(s) 115
MboI GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
MboII GAAGA 6 cut(s) 91, 166, 537, 570, 924, 938
MflI RGATCY 4 cut(s) 133, 186, 273, 396
MhlI GDGCHC 3 cut(s) 11, 62, 288
MluCI AATT 4 cut(s) 404, 519, 796, 909
MlyI GAGTC 4 cut(s) 445, 613, 727, 923
MseI TTAA 4 cut(s) 560, 659, 695, 830
MspA1I CMGCKG 1 cut(s) 267
MspR9I CCNGG 4 cut(s) 278, 313, 624, 690
MvaI CCWGG 4 cut(s) 278, 313, 624, 690
MwoI GCNNNNNNNGC 5 cut(s) 102, 200, 203, 368, 911
NdeII GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
NlaIII CATG 7 cut(s) 211, 330, 451, 544, 619, 740, 772
NmuCI GTSAC 1 cut(s) 626
NspI RCATGY 2 cut(s) 330, 772
PagI TCATGA 3 cut(s) 447, 615, 736
PciI ACATGT 1 cut(s) 768
PfeI GAWTC 2 cut(s) 163, 383
PfoI TCCNGGA 1 cut(s) 276
PkrI GCNGC 6 cut(s) 196, 205, 263, 266, 745, 805
PleI GAGTC 4 cut(s) 445, 613, 727, 923
PmaCI CACGTG 1 cut(s) 126
PmlI CACGTG 1 cut(s) 126
PpsI GAGTC 4 cut(s) 445, 613, 727, 923
Ppu21I YACGTR 2 cut(s) 126, 292
PscI ACATGT 1 cut(s) 768
PshBI ATTAAT 1 cut(s) 830
Psp124BI GAGCTC 1 cut(s) 62
Psp1406I AACGTT 1 cut(s) 786
Psp6I CCWGG 4 cut(s) 276, 311, 622, 688
PspCI CACGTG 1 cut(s) 126
PspFI CCCAGC 1 cut(s) 75
PspGI CCWGG 4 cut(s) 276, 311, 622, 688
PspPI GGNCC 2 cut(s) 391, 904
PstI CTGCAG 1 cut(s) 266
PsuI RGATCY 4 cut(s) 133, 186, 273, 396
SacI GAGCTC 1 cut(s) 62
SaqAI TTAA 4 cut(s) 560, 659, 695, 830
SatI GCNGC 6 cut(s) 195, 204, 262, 265, 744, 804
Sau3AI GATC 7 cut(s) 81, 133, 156, 186, 273, 396, 512
Sau96I GGNCC 2 cut(s) 391, 904
SchI GAGTC 4 cut(s) 445, 613, 727, 923
ScrFI CCNGG 4 cut(s) 278, 313, 624, 690
SduI GDGCHC 3 cut(s) 11, 62, 288
SfaNI GCATC 1 cut(s) 890
SfcI CTRYAG 3 cut(s) 262, 376, 998
SmlI CTYRAG 1 cut(s) 899
SmoI CTYRAG 1 cut(s) 899
SnaBI TACGTA 1 cut(s) 292
Sse9I AATT 4 cut(s) 404, 519, 796, 909
SsiI CCGC 7 cut(s) 5, 113, 192, 195, 201, 204, 267
SspMI CTAG 1 cut(s) 348
SstI GAGCTC 1 cut(s) 62
StyD4I CCNGG 4 cut(s) 276, 311, 622, 688
StyI CCWWGG 2 cut(s) 600, 885
TaaI ACNGT 3 cut(s) 171, 256, 842
TaiI ACGT 4 cut(s) 128, 246, 294, 789
TaqI TCGA 5 cut(s) 84, 166, 296, 415, 960
TasI AATT 4 cut(s) 404, 519, 796, 909
TauI GCSGC 2 cut(s) 197, 206
TfiI GAWTC 2 cut(s) 163, 383
Tru1I TTAA 4 cut(s) 560, 659, 695, 830
Tru9I TTAA 4 cut(s) 560, 659, 695, 830
TseFI GTSAC 1 cut(s) 626
TseI GCWGC 4 cut(s) 261, 264, 743, 803
Tsp45I GTSAC 1 cut(s) 626
VspI ATTAAT 1 cut(s) 830
XapI RAATTY 2 cut(s) 404, 519
XceI RCATGY 2 cut(s) 330, 772
XspI CTAG 1 cut(s) 348
ZraI GACGTC 1 cut(s) 244
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.