Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000759.1

Orthogroup ID

OG0008767

Physical Location & Seq

Genomic Coordinates Forward (+)

67778 .. 70511

Gene Structure 2734 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0000759.1_g000014.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1146 bp

atgcatggtctctttatagaaagtgctggggacgtggcgacctggtccagtcacgcgatccgcacctcaggccgttactataaattcactgcattccactccatttcctcaccatttcaaacacgtttcgtttctccaaaaatccagacaagcacagagcctgaaaaaaaaatggcgggcactctggctttgataccaagcaatccgccggtgacgtcatcgtctctgagctccatccgagagcggtgggaggtcgagttcgctcgcttcttcggctaccctccgctcacctccacgtgtccagatctcgtccctcttcctatgaaggtcaggaatcgacggccagtcggtagctggatctccgccgcctccgccgccatgcttcaactcatttatgaccactccagctccgacgtcatcctcaccgtctgctccagcggcaagatcatggaggagcactacgtgtcgaagctactcttttcctggcctcaggttgcatgcatgtctggatatcctgccaggggcagtagagccatctttgccagctacagagattctttgggccagatccagaaatttgcttttcgatttttgtcagtcaatgaagcagagaagttcataactcttatgaaggagattttcaaggaagggagggatattcaacctcttaatactgactatggatctgaaatttcatcacaatctgagttcatgtcttccaatagacctctaaacagaacttccaaggacttggttgtcatgactcctgttcacagtcattctcctcaaatatcaccaagcttaatgacggcttatactcctcaaatgtcaccaagcttaatgaaggattatacacctcaaatgtcaccaacctggaaacatgaagcagctcaacacagacaaacggagcacatgtcacctattcagaactttcagagcaactttgctgcctttcctcctagtttcacctcattagtgtccaactgtcaacctctggttgagcaagctccagcacaatcaactgcatcaaaggaagtcgatctcaaggccctaattgcgagatgtatggaagactcttccttccaagatatattgagccaagttgagacagttatgggcgaaatgggggctgatttgaagctgtag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000280 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0008150 GO:0009987 GO:0016043 GO:0022402 GO:0022414 GO:0044424 GO:0044464 GO:0045132 GO:0045143 GO:0048285 GO:0051276 GO:0051321 GO:0061982 GO:0070192 GO:0071840 GO:0098813 GO:0140013 GO:1903046

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

No domains found.

Protein Analysis

381

Amino Acids

42.5

Weight (kDa)

7.7

Isoelectric Point (pI)

60.83

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008767)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10710 AT1G10710
fragaria_vesca	FvH4_4g25900 FvH4_4g25900 FvH4_4g25900
malus_domestica	MD13G1097800.v1.1 MD13G1097900.v1.1
prunus_persica	Prupe.1G247500_v2.0.a1 Prupe.1G247600_v2.0.a1 Prupe.1G247600_v2.0.a1
pyrus_communis	pycom13g08550
rosa_chinensis	RchiOBHm_Chr4g0433351
rosa_laevigata	RLG00000006761
rosa_multiflora	Rmu_sc0000354.1_g000013 Rmu_sc0000354.1_g000014 Rmu_sc0000759.1_g000014
rosa_roxburghii	Rroxscaffold_5G00374900
rosa_rugosa	Rorug04G0268000
rosa_samantha	Rh4AG322800 Rh4BG330500 Rh4CG345900 Rh4DG327000
rosa_wichuraiana	Rw4G027940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	220, 755
AatII	GACGTC	2 cut(s)	218, 417
AccBSI	CCGCTC	2 cut(s)	244, 286
AccII	CGCG	1 cut(s)	56
AclWI	GGATC	4 cut(s)	52, 365, 562, 691
AcoI	YGGCCR	1 cut(s)	341
AcsI	RAATTY	3 cut(s)	83, 575, 690
AcvI	CACGTG	1 cut(s)	297
AcyI	GRCGYC	2 cut(s)	215, 414
AdeI	CACNNNGTG	1 cut(s)	463
AfiI	CCNNNNNNNGG	1 cut(s)	521
AflIII	ACRYGT	4 cut(s)	122, 296, 462, 912
AgsI	TTSAA	5 cut(s)	119, 386, 643, 662, 1138
AhdI	GACNNNNNGTC	1 cut(s)	344
AjiI	CACGTC	1 cut(s)	34
AjnI	CCWGG	4 cut(s)	41, 482, 518, 872
AloI	GAACNNNNNNTCC	2 cut(s)	242, 274
Alw21I	GWGCWC	3 cut(s)	233, 459, 912
Alw26I	GTCTC	3 cut(s)	14, 228, 1100
AlwI	GGATC	4 cut(s)	52, 365, 562, 691
AlwNI	CAGNNNCTG	1 cut(s)	161
AoxI	GGCC	5 cut(s)	70, 341, 485, 562, 1047
ApeKI	GCWGC	2 cut(s)	887, 947
ApoI	RAATTY	3 cut(s)	83, 575, 690
AspS9I	GGNCC	3 cut(s)	45, 562, 1048
AsuHPI	GGTGA	9 cut(s)	102, 223, 280, 415, 786, 822, 858, 909, 958
AvaII	GGWCC	1 cut(s)	45
AxyI	CCTNAGG	2 cut(s)	67, 489
BaeGI	GKGCMC	1 cut(s)	182
BanII	GRGCYC	1 cut(s)	233
BbrPI	CACGTG	1 cut(s)	297
BbsI	GAAGAC	2 cut(s)	708, 1077
Bbv12I	GWGCWC	3 cut(s)	233, 459, 912
BbvI	GCAGC	2 cut(s)	899, 934
BccI	CCATC	2 cut(s)	242, 542
BceAI	ACGGC	3 cut(s)	57, 356, 825
BciT130I	CCWGG	4 cut(s)	43, 484, 520, 874
BcoDI	GTCTC	3 cut(s)	14, 228, 1100
BfaI	CTAG	1 cut(s)	960
BfmI	CTRYAG	2 cut(s)	547, 1142
BglII	AGATCT	1 cut(s)	304
BisI	GCNGC	5 cut(s)	366, 375, 439, 888, 948
BlsI	GCNGC	5 cut(s)	367, 376, 440, 889, 949
Bme1390I	CCNGG	4 cut(s)	43, 484, 520, 874
Bme18I	GGWCC	1 cut(s)	45
BmeRI	GACNNNNNGTC	1 cut(s)	344
BmgBI	CACGTC	1 cut(s)	34
BmgT120I	GGNCC	3 cut(s)	45, 562, 1048
BmrFI	CCNGG	4 cut(s)	43, 484, 520, 874
BmsI	GCATC	1 cut(s)	1034
BpiI	GAAGAC	2 cut(s)	708, 1077
BpmI	CTGGAG	3 cut(s)	388, 418, 993
BpuEI	CTTGAG	1 cut(s)	1028
BsaAI	YACGTR	2 cut(s)	297, 463
BsaHI	GRCGYC	2 cut(s)	215, 414
BsaI	GGTCTC	1 cut(s)	14
BsaJI	CCNNGG	2 cut(s)	519, 744
BsaXI	ACNNNNNCTCC	6 cut(s)	242, 272, 392, 422, 899, 929
Bsc4I	CCNNNNNNNGG	1 cut(s)	521
Bse118I	RCCGGY	1 cut(s)	208
Bse1I	ACTGG	2 cut(s)	48, 344
Bse21I	CCTNAGG	2 cut(s)	67, 489
BseBI	CCWGG	4 cut(s)	43, 484, 520, 874
BseDI	CCNNGG	2 cut(s)	519, 744
BseGI	GGATG	2 cut(s)	234, 417
BseLI	CCNNNNNNNGG	1 cut(s)	521
BseMII	CTCAG	4 cut(s)	81, 218, 503, 696
BseNI	ACTGG	2 cut(s)	48, 344
BseRI	GAGGAG	3 cut(s)	467, 774, 810
BseSI	GKGCMC	1 cut(s)	182
BseXI	GCAGC	2 cut(s)	899, 934
BseYI	CCCAGC	1 cut(s)	26
Bsh1236I	CGCG	1 cut(s)	56
BshFI	GGCC	5 cut(s)	72, 343, 487, 564, 1049
BsiHKAI	GWGCWC	3 cut(s)	233, 459, 912
BsiSI	CCGG	1 cut(s)	209
BslFI	GGGAC	2 cut(s)	44, 296
BslI	CCNNNNNNNGG	1 cut(s)	521
BsmAI	GTCTC	3 cut(s)	14, 228, 1100
BsmBI	CGTCTC	1 cut(s)	228
BsmFI	GGGAC	2 cut(s)	44, 296
BsmI	GAATGC	1 cut(s)	92
BsnI	GGCC	5 cut(s)	72, 343, 487, 564, 1049
Bso31I	GGTCTC	1 cut(s)	14
Bsp1286I	GDGCHC	4 cut(s)	182, 233, 459, 912
Bsp143I	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
BspANI	GGCC	5 cut(s)	72, 343, 487, 564, 1049
BspCNI	CTCAG	4 cut(s)	80, 219, 502, 697
BspFNI	CGCG	1 cut(s)	56
BspHI	TCATGA	1 cut(s)	759
BspPI	GGATC	4 cut(s)	52, 365, 562, 691
BspTNI	GGTCTC	1 cut(s)	14
BsrBI	CCGCTC	2 cut(s)	244, 286
BsrFI	RCCGGY	1 cut(s)	208
BsrI	ACTGG	2 cut(s)	48, 344
BssAI	RCCGGY	1 cut(s)	208
BssECI	CCNNGG	2 cut(s)	519, 744
BssMI	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
BssNI	GRCGYC	2 cut(s)	215, 414
BssT1I	CCWWGG	1 cut(s)	744
Bst2UI	CCWGG	4 cut(s)	43, 484, 520, 874
Bst4CI	ACNGT	4 cut(s)	427, 776, 986, 1111
Bst6I	CTCTTC	2 cut(s)	321, 1081
BstACI	GRCGYC	2 cut(s)	215, 414
BstBAI	YACGTR	2 cut(s)	297, 463
BstC8I	GCNNGC	5 cut(s)	178, 265, 499, 544, 1005
BstDEI	CTNAG	4 cut(s)	67, 227, 489, 705
BstF5I	GGATG	2 cut(s)	234, 417
BstFNI	CGCG	1 cut(s)	56
BstKTI	GATC	7 cut(s)	60, 307, 360, 447, 570, 686, 1042
BstMAI	GTCTC	3 cut(s)	14, 228, 1100
BstMBI	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
BstMWI	GCNNNNNNNGC	7 cut(s)	69, 273, 371, 374, 438, 539, 1055
BstNI	CCWGG	4 cut(s)	43, 484, 520, 874
BstNSI	RCATGY	3 cut(s)	501, 505, 916
BstSCI	CCNGG	4 cut(s)	41, 482, 518, 872
BstSFI	CTRYAG	2 cut(s)	547, 1142
BstSLI	GKGCMC	1 cut(s)	182
BstUI	CGCG	1 cut(s)	56
BstV1I	GCAGC	2 cut(s)	899, 934
BstV2I	GAAGAC	2 cut(s)	708, 1077
BstX2I	RGATCY	4 cut(s)	304, 357, 567, 683
BstXI	CCANNNNNNTGG	1 cut(s)	751
BstYI	RGATCY	4 cut(s)	304, 357, 567, 683
Bsu36I	CCTNAGG	2 cut(s)	67, 489
BsuRI	GGCC	5 cut(s)	72, 343, 487, 564, 1049
BtrI	CACGTC	1 cut(s)	34
BtsCI	GGATG	2 cut(s)	234, 417
BtsI	GCAGTG	1 cut(s)	87
BtsIMutI	CAGTG	1 cut(s)	87
Cac8I	GCNNGC	5 cut(s)	178, 265, 499, 544, 1005
CaiI	CAGNNNCTG	1 cut(s)	161
CciI	TCATGA	1 cut(s)	759
Cfr10I	RCCGGY	1 cut(s)	208
Cfr13I	GGNCC	3 cut(s)	45, 562, 1048
CsiI	ACCWGGT	1 cut(s)	41
CviAII	CATG	9 cut(s)	5, 379, 448, 498, 502, 712, 760, 881, 913
DdeI	CTNAG	4 cut(s)	67, 227, 489, 705
DpnI	GATC	7 cut(s)	59, 306, 359, 446, 569, 685, 1041
DpnII	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
DraIII	CACNNNGTG	1 cut(s)	463
DrdI	GACNNNNNNGTC	2 cut(s)	220, 755
DriI	GACNNNNNGTC	1 cut(s)	344
DseDI	GACNNNNNNGTC	2 cut(s)	220, 755
EaeI	YGGCCR	1 cut(s)	341
Eam1104I	CTCTTC	2 cut(s)	321, 1081
Eam1105I	GACNNNNNGTC	1 cut(s)	344
EarI	CTCTTC	2 cut(s)	321, 1081
EciI	GGCGGA	3 cut(s)	195, 352, 361
Ecl136II	GAGCTC	1 cut(s)	231
Eco130I	CCWWGG	1 cut(s)	744
Eco24I	GRGCYC	1 cut(s)	233
Eco31I	GGTCTC	1 cut(s)	14
Eco32I	GATATC	1 cut(s)	512
Eco47I	GGWCC	1 cut(s)	45
Eco53kI	GAGCTC	1 cut(s)	231
Eco72I	CACGTG	1 cut(s)	297
Eco81I	CCTNAGG	2 cut(s)	67, 489
EcoICRI	GAGCTC	1 cut(s)	231
EcoO109I	RGGNCCY	1 cut(s)	1048
EcoRII	CCWGG	4 cut(s)	41, 482, 518, 872
EcoRV	GATATC	1 cut(s)	512
EcoT14I	CCWWGG	1 cut(s)	744
EcoT22I	ATGCAT	2 cut(s)	6, 503
EcoT38I	GRGCYC	1 cut(s)	233
ErhI	CCWWGG	1 cut(s)	744
Esp3I	CGTCTC	1 cut(s)	228
FaeI	CATG	9 cut(s)	8, 382, 451, 501, 505, 715, 763, 884, 916
FalI	AAGNNNNNCTT	2 cut(s)	461, 493
FaqI	GGGAC	2 cut(s)	44, 296
FatI	CATG	9 cut(s)	4, 378, 447, 497, 501, 711, 759, 880, 912
FauI	CCCGC	1 cut(s)	169
Fnu4HI	GCNGC	5 cut(s)	366, 375, 439, 888, 948
FokI	GGATG	2 cut(s)	221, 404
FriOI	GRGCYC	1 cut(s)	233
Fsp4HI	GCNGC	5 cut(s)	366, 375, 439, 888, 948
FspBI	CTAG	1 cut(s)	960
GluI	GCNGC	5 cut(s)	366, 375, 439, 888, 948
GsaI	CCCAGC	1 cut(s)	30
GsuI	CTGGAG	3 cut(s)	388, 418, 993
HaeIII	GGCC	5 cut(s)	72, 343, 487, 564, 1049
HapII	CCGG	1 cut(s)	209
Hin1I	GRCGYC	2 cut(s)	215, 414
Hin1II	CATG	9 cut(s)	8, 382, 451, 501, 505, 715, 763, 884, 916
HincII	GTYRAC	1 cut(s)	989
HindII	GTYRAC	1 cut(s)	989
HindIII	AAGCTT	2 cut(s)	799, 835
HinfI	GANTC	4 cut(s)	334, 554, 763, 1073
HpaII	CCGG	1 cut(s)	209
HphI	GGTGA	9 cut(s)	102, 223, 280, 415, 786, 822, 858, 909, 958
Hpy166II	GTNNAC	2 cut(s)	772, 989
Hpy188I	TCNGA	7 cut(s)	228, 239, 412, 688, 706, 927, 936
Hpy188III	TCNNGA	6 cut(s)	145, 302, 331, 507, 571, 760
Hpy8I	GTNNAC	2 cut(s)	772, 989
Hpy99I	CGWCG	2 cut(s)	342, 416
HpyAV	CCTTC	5 cut(s)	319, 625, 641, 838, 1090
HpyCH4III	ACNGT	4 cut(s)	427, 776, 986, 1111
HpyCH4IV	ACGT	6 cut(s)	33, 124, 215, 296, 414, 462
HpyCH4V	TGCA	5 cut(s)	4, 92, 497, 501, 1025
HpyF10VI	GCNNNNNNNGC	7 cut(s)	69, 273, 371, 374, 438, 539, 1055
HpyF3I	CTNAG	4 cut(s)	67, 227, 489, 705
HpySE526I	ACGT	6 cut(s)	33, 124, 215, 296, 414, 462
Hsp92I	GRCGYC	2 cut(s)	215, 414
Hsp92II	CATG	9 cut(s)	8, 382, 451, 501, 505, 715, 763, 884, 916
Kzo9I	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
LmnI	GCTCC	6 cut(s)	236, 413, 437, 454, 907, 1012
Lsp1109I	GCAGC	2 cut(s)	899, 934
LweI	GCATC	1 cut(s)	1034
MabI	ACCWGGT	1 cut(s)	41
MaeI	CTAG	1 cut(s)	960
MaeII	ACGT	6 cut(s)	33, 124, 215, 296, 414, 462
MaeIII	GTNAC	6 cut(s)	50, 74, 211, 828, 864, 915
MalI	GATC	7 cut(s)	59, 306, 359, 446, 569, 685, 1041
MbiI	CCGCTC	2 cut(s)	244, 286
MboI	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
MboII	GAAGA	5 cut(s)	262, 308, 708, 1068, 1082
MflI	RGATCY	4 cut(s)	304, 357, 567, 683
MhlI	GDGCHC	4 cut(s)	182, 233, 459, 912
MluCI	AATT	4 cut(s)	83, 575, 690, 1053
MlyI	GAGTC	2 cut(s)	757, 1067
MmeI	TCCRAC	2 cut(s)	435, 1005
Mph1103I	ATGCAT	2 cut(s)	6, 503
MseI	TTAA	3 cut(s)	669, 803, 839
MspA1I	CMGCKG	1 cut(s)	438
MspI	CCGG	1 cut(s)	209
MspR9I	CCNGG	4 cut(s)	43, 484, 520, 874
Mva1269I	GAATGC	1 cut(s)	92
MvaI	CCWGG	4 cut(s)	43, 484, 520, 874
MvnI	CGCG	1 cut(s)	56
MwoI	GCNNNNNNNGC	7 cut(s)	69, 273, 371, 374, 438, 539, 1055
NdeII	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
NlaIII	CATG	9 cut(s)	8, 382, 451, 501, 505, 715, 763, 884, 916
NmuCI	GTSAC	5 cut(s)	50, 211, 828, 864, 915
NsiI	ATGCAT	2 cut(s)	6, 503
NspI	RCATGY	3 cut(s)	501, 505, 916
PaeI	GCATGC	1 cut(s)	501
PagI	TCATGA	1 cut(s)	759
PciI	ACATGT	1 cut(s)	912
PctI	GAATGC	1 cut(s)	92
PfeI	GAWTC	2 cut(s)	334, 554
PflFI	GACNNNGTC	1 cut(s)	43
PkrI	GCNGC	5 cut(s)	367, 376, 440, 889, 949
PleI	GAGTC	2 cut(s)	757, 1067
PmaCI	CACGTG	1 cut(s)	297
PmlI	CACGTG	1 cut(s)	297
PpsI	GAGTC	2 cut(s)	757, 1067
Ppu21I	YACGTR	2 cut(s)	297, 463
PscI	ACATGT	1 cut(s)	912
Psp124BI	GAGCTC	1 cut(s)	233
Psp6I	CCWGG	4 cut(s)	41, 482, 518, 872
PspCI	CACGTG	1 cut(s)	297
PspFI	CCCAGC	1 cut(s)	26
PspGI	CCWGG	4 cut(s)	41, 482, 518, 872
PspPI	GGNCC	3 cut(s)	45, 562, 1048
PstNI	CAGNNNCTG	1 cut(s)	161
PsuI	RGATCY	4 cut(s)	304, 357, 567, 683
PsyI	GACNNNGTC	1 cut(s)	43
SacI	GAGCTC	1 cut(s)	233
SaqAI	TTAA	3 cut(s)	669, 803, 839
SatI	GCNGC	5 cut(s)	366, 375, 439, 888, 948
Sau3AI	GATC	7 cut(s)	57, 304, 357, 444, 567, 683, 1039
Sau96I	GGNCC	3 cut(s)	45, 562, 1048
SchI	GAGTC	2 cut(s)	757, 1067
ScrFI	CCNGG	4 cut(s)	43, 484, 520, 874
SduI	GDGCHC	4 cut(s)	182, 233, 459, 912
SexAI	ACCWGGT	1 cut(s)	41
SfaNI	GCATC	1 cut(s)	1034
SfcI	CTRYAG	2 cut(s)	547, 1142
SgrAI	CRCCGGYG	1 cut(s)	208
SinI	GGWCC	1 cut(s)	45
SmlI	CTYRAG	1 cut(s)	1043
SmoI	CTYRAG	1 cut(s)	1043
SphI	GCATGC	1 cut(s)	501
Sse9I	AATT	4 cut(s)	83, 575, 690, 1053
SspMI	CTAG	1 cut(s)	960
SstI	GAGCTC	1 cut(s)	233
StyD4I	CCNGG	4 cut(s)	41, 482, 518, 872
StyI	CCWWGG	1 cut(s)	744
TaaI	ACNGT	4 cut(s)	427, 776, 986, 1111
TaiI	ACGT	6 cut(s)	36, 127, 218, 299, 417, 465
TaqI	TCGA	5 cut(s)	255, 337, 467, 586, 1038
TasI	AATT	4 cut(s)	83, 575, 690, 1053
TauI	GCSGC	3 cut(s)	368, 377, 441
TfiI	GAWTC	2 cut(s)	334, 554
Tru1I	TTAA	3 cut(s)	669, 803, 839
Tru9I	TTAA	3 cut(s)	669, 803, 839
TscAI	CASTG	1 cut(s)	94
TseFI	GTSAC	5 cut(s)	50, 211, 828, 864, 915
TseI	GCWGC	2 cut(s)	887, 947
Tsp45I	GTSAC	5 cut(s)	50, 211, 828, 864, 915
TspDTI	ATGAA	8 cut(s)	338, 607, 618, 644, 684, 700, 857, 897
TspGWI	ACGGA	1 cut(s)	920
TspRI	CASTG	1 cut(s)	94
Tth111I	GACNNNGTC	1 cut(s)	43
VpaK11BI	GGWCC	1 cut(s)	45
XapI	RAATTY	3 cut(s)	83, 575, 690
XceI	RCATGY	3 cut(s)	501, 505, 916
XcmI	CCANNNNNNNNNTGG	1 cut(s)	351
XspI	CTAG	1 cut(s)	960
ZraI	GACGTC	2 cut(s)	216, 415
Zsp2I	ATGCAT	2 cut(s)	6, 503

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.