FvH4_4g26020

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014950
Physical Location & Seq
Forward (+)
27598131 .. 27600608
2478 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g26020.t1

Sequence Viewer

Length: 1170 bp
ATGGAAACTCAGTTTCTAAGACATGTTGCCTTTGGGGTTGTGATTTGGGCAACATTGCTTCTGTATCCTAGCACTTGTTCAAAGCCATGTTCTTTTCCGGCCATATTCAACTTTGGAGACTCAAACTCAGACACCGGGGGTCTGTCCGCAACGTTTGGACAAGCCCCTTACCCCAATGGAGAGACATATTTTCGAACACCTTCAGGCCGTTATTCCGATGGCCGTCTTATAATTGACTTCATAGCCGAAACCTTGAGGTTACCTCATCTGAGTGCATTTTTGGACTCGGTTGGATCAAACTTCAGCCATGGAGTGAATTTTGCAACTGCAGGATCAACCATTAGACCCCCAAACATGACCATCCAATATGGACCTAGTCCCATTTCATTAGATGTGCAGTCTGTCGAATTCTCAGACTTCCATAGAAGATCACAAATTTACCGCCAAAAAGGGGAGTTTTTTGAGAAACTGCTTCCAAGAGAAGAGTTCTATTCTCAAGCACTTTATACCTTTGACATTGGCCAGAATGATCTCACTGCTGGTTACAAGCTTAACATGAGCATAGAACAAATCAAGGCCTATGTTCCTGATGTGCTAGGCCAGTTCTCTAATGTCATCAAGAATATATACGATCAAGGAGGAAGAACGTTTTGGATCCACAACACAGGCCCAGTAGGCTGCTACCCCTATATTCTGGATCGTTTCTTTGTCAATCGGGCCCAATATAACAAGTATGGGTGTGCAGATCCGTTTAATGATGTGGCCCAATTTTTCAACCAAAGATTGAAGGAAGCAGTGGTTCAGCTAGAGAAAGATCTTCCCATGGCTGCCTTCACCTACGTGGATGTCTACTCAGTAAAGTACACCCTCATTACTGAAGCCAAGAAATATGGATTTGAGAAGCCACTTGTAGCATGTTGTGGTCATGGAGGGAAATACAATTACAACCGTTACGAGAAGTGTGGGACGAAGAAGACTATCGACGGCAGAGAGGTAGTGATCGCTAACTCATGCAATGATCCAACGATTAGGGTTAATTGGGATGGGACGCATTTCACCGAGGCTGCCAACAAATGGATATTCCAACAAATTGTAAATGGCTCCTTTTCTTATCCACCAAACCCTTTGGAAATGGCTTGTCAAAGAAGAAACACAAAGAACTATGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

390

Amino Acids

44.12

Weight (kDa)

7.97

Isoelectric Point (pI)

31.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 364 2.3e-44 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26430
fragaria_vesca FvH4_4g26020
malus_domestica MD16G1100400.v1.1
prunus_persica Prupe.1G246600_v2.0.a1 Prupe.1G246600_v2.0.a1
pyrus_communis pycom16g08510
rosa_chinensis RchiOBHm_Chr4g0433461
rosa_laevigata RLG00000006751
rosa_multiflora Rmu_sc0000312.1_g000001
rosa_roxburghii Rroxscaffold_5G00375000
rosa_rugosa Rorug04G0269000
rosa_samantha Rh4BG331600 Rh4CG346900 Rh4DG327900
rosa_wichuraiana Rw0G020090 Rw4G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 1074
AccI GTMKAC 1 cut(s) 849
AciI CCGC 2 cut(s) 147, 442
AclI AACGTT 2 cut(s) 152, 647
AclWI GGATC 7 cut(s) 301, 340, 649, 662, 705, 740, 1013
AcoI YGGCCR 3 cut(s) 99, 220, 520
AcsI RAATTY 3 cut(s) 316, 407, 435
AcuI CTGAAG 3 cut(s) 186, 286, 897
AfaI GTAC 1 cut(s) 863
AfiI CCNNNNNNNGG 2 cut(s) 451, 1074
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 4 cut(s) 81, 109, 775, 787
AjuI GAANNNNNNNTTGG 2 cut(s) 634, 666
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 2 cut(s) 550, 805
AluI AGCT 2 cut(s) 550, 805
Alw26I GTCTC 2 cut(s) 111, 176
AlwI GGATC 7 cut(s) 301, 340, 649, 662, 705, 740, 1013
AoxI GGCC 9 cut(s) 99, 205, 220, 520, 576, 598, 667, 717, 762
ApaI GGGCCC 1 cut(s) 721
ApeKI GCWGC 3 cut(s) 678, 827, 1064
ApoI RAATTY 3 cut(s) 316, 407, 435
Asp700I GAANNNNTTC 1 cut(s) 199
AspS9I GGNCC 5 cut(s) 371, 668, 717, 718, 763
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 2 cut(s) 826, 1048
AsuII TTCGAA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 721
BalI TGGCCA 1 cut(s) 522
BamHI GGATCC 1 cut(s) 654
BanII GRGCYC 1 cut(s) 721
BbsI GAAGAC 1 cut(s) 980
BbvI GCAGC 3 cut(s) 665, 814, 1051
BccI CCATC 3 cut(s) 212, 368, 1037
BceAI ACGGC 3 cut(s) 192, 207, 1000
BciVI GTATCC 1 cut(s) 75
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 2 cut(s) 111, 176
BfaI CTAG 4 cut(s) 69, 375, 596, 806
BfmI CTRYAG 1 cut(s) 327
BfuI GTATCC 1 cut(s) 75
BglI GCCNNNNNGGC 1 cut(s) 675
BglII AGATCT 1 cut(s) 814
BisI GCNGC 3 cut(s) 679, 828, 1065
BlsI GCNGC 3 cut(s) 680, 829, 1066
Bme1390I CCNGG 1 cut(s) 136
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 5 cut(s) 371, 668, 717, 718, 763
BmiI GGNNCC 3 cut(s) 656, 719, 1102
BmrFI CCNGG 1 cut(s) 136
BmrI ACTGGG 1 cut(s) 665
BmuI ACTGGG 1 cut(s) 665
BpiI GAAGAC 1 cut(s) 980
BplI GAGNNNNNCTC 2 cut(s) 247, 279
Bpu14I TTCGAA 1 cut(s) 193
BpuEI CTTGAG 2 cut(s) 274, 480
BpuMI CCSGG 1 cut(s) 136
BsaAI YACGTR 1 cut(s) 841
BsaJI CCNNGG 4 cut(s) 135, 307, 822, 1059
Bsc4I CCNNNNNNNGG 2 cut(s) 451, 1074
Bse1I ACTGG 2 cut(s) 601, 671
Bse3DI GCAATG 2 cut(s) 53, 1021
BseDI CCNNGG 4 cut(s) 135, 307, 822, 1059
BseGI GGATG 3 cut(s) 360, 850, 1048
BseLI CCNNNNNNNGG 2 cut(s) 451, 1074
BseMI GCAATG 2 cut(s) 53, 1021
BseMII CTCAG 5 cut(s) 23, 141, 260, 426, 867
BseNI ACTGG 2 cut(s) 601, 671
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 3 cut(s) 665, 814, 1051
BsgI GTGCAG 2 cut(s) 416, 762
BshFI GGCC 9 cut(s) 101, 207, 222, 522, 578, 600, 669, 719, 764
BsiSI CCGG 2 cut(s) 98, 135
BslFI GGGAC 3 cut(s) 363, 979, 1060
BslI CCNNNNNNNGG 2 cut(s) 451, 1074
BsmAI GTCTC 2 cut(s) 111, 176
BsmFI GGGAC 3 cut(s) 363, 979, 1060
BsnI GGCC 9 cut(s) 101, 207, 222, 522, 578, 600, 669, 719, 764
Bsp119I TTCGAA 1 cut(s) 193
Bsp120I GGGCCC 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 721
Bsp19I CCATGG 2 cut(s) 307, 822
BspACI CCGC 2 cut(s) 147, 442
BspANI GGCC 9 cut(s) 101, 207, 222, 522, 578, 600, 669, 719, 764
BspCNI CTCAG 5 cut(s) 22, 140, 261, 425, 866
BspLI GGNNCC 3 cut(s) 656, 719, 1102
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 7 cut(s) 301, 340, 649, 662, 705, 740, 1013
BspT104I TTCGAA 1 cut(s) 193
BsrDI GCAATG 2 cut(s) 53, 1021
BsrI ACTGG 2 cut(s) 601, 671
BssECI CCNNGG 4 cut(s) 135, 307, 822, 1059
BssT1I CCWWGG 2 cut(s) 307, 822
Bst4CI ACNGT 1 cut(s) 950
Bst6I CTCTTC 1 cut(s) 477
BstBAI YACGTR 1 cut(s) 841
BstBI TTCGAA 1 cut(s) 193
BstDEI CTNAG 6 cut(s) 9, 17, 127, 269, 412, 853
BstDSI CCRYGG 2 cut(s) 307, 822
BstEII GGTNACC 1 cut(s) 258
BstF5I GGATG 3 cut(s) 360, 850, 1048
BstMAI GTCTC 2 cut(s) 111, 176
BstMWI GCNNNNNNNGC 1 cut(s) 675
BstNSI RCATGY 2 cut(s) 26, 918
BstPI GGTNACC 1 cut(s) 258
BstSCI CCNGG 1 cut(s) 134
BstSFI CTRYAG 1 cut(s) 327
BstSLI GKGCMC 1 cut(s) 721
BstV1I GCAGC 3 cut(s) 665, 814, 1051
BstV2I GAAGAC 1 cut(s) 980
BstX2I RGATCY 3 cut(s) 654, 745, 814
BstYI RGATCY 3 cut(s) 654, 745, 814
BsuI GTATCC 1 cut(s) 75
BsuRI GGCC 9 cut(s) 101, 207, 222, 522, 578, 600, 669, 719, 764
BtgI CCRYGG 2 cut(s) 307, 822
BtsCI GGATG 3 cut(s) 360, 850, 1048
BtsI GCAGTG 2 cut(s) 534, 801
BtsIMutI CAGTG 2 cut(s) 534, 801
Cfr13I GGNCC 5 cut(s) 371, 668, 717, 718, 763
CseI GACGC 1 cut(s) 1057
Csp6I GTAC 1 cut(s) 862
CviAII CATG 9 cut(s) 23, 87, 308, 355, 556, 823, 915, 926, 1011
CviQI GTAC 1 cut(s) 862
DdeI CTNAG 6 cut(s) 9, 17, 127, 269, 412, 853
EaeI YGGCCR 3 cut(s) 99, 220, 520
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 307, 822
Eco147I AGGCCT 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 721
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 3 cut(s) 186, 286, 897
Eco91I GGTNACC 1 cut(s) 258
EcoO65I GGTNACC 1 cut(s) 258
EcoRI GAATTC 1 cut(s) 407
EcoT14I CCWWGG 2 cut(s) 307, 822
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 2 cut(s) 307, 822
FaeI CATG 9 cut(s) 26, 90, 311, 358, 559, 826, 918, 929, 1014
FaqI GGGAC 3 cut(s) 363, 979, 1060
FatI CATG 9 cut(s) 22, 86, 307, 354, 555, 822, 914, 925, 1010
FblI GTMKAC 1 cut(s) 849
Fnu4HI GCNGC 3 cut(s) 679, 828, 1065
FokI GGATG 3 cut(s) 347, 857, 1055
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 3 cut(s) 679, 828, 1065
FspBI CTAG 4 cut(s) 69, 375, 596, 806
GluI GCNGC 3 cut(s) 679, 828, 1065
HaeIII GGCC 9 cut(s) 101, 207, 222, 522, 578, 600, 669, 719, 764
HapII CCGG 2 cut(s) 98, 135
HgaI GACGC 1 cut(s) 1057
Hin1II CATG 9 cut(s) 26, 90, 311, 358, 559, 826, 918, 929, 1014
HindIII AAGCTT 1 cut(s) 548
HinfI GANTC 2 cut(s) 119, 284
HpaII CCGG 2 cut(s) 98, 135
HphI GGTGA 2 cut(s) 826, 1048
Hpy166II GTNNAC 2 cut(s) 850, 864
Hpy188I TCNGA 4 cut(s) 130, 217, 270, 415
Hpy188III TCNNGA 3 cut(s) 587, 619, 695
Hpy8I GTNNAC 2 cut(s) 850, 864
Hpy99I CGWCG 1 cut(s) 986
HpyAV CCTTC 3 cut(s) 210, 781, 841
HpyCH4III ACNGT 1 cut(s) 950
HpyCH4IV ACGT 3 cut(s) 152, 647, 840
HpyCH4V TGCA 6 cut(s) 275, 323, 329, 397, 743, 1014
HpyF10VI GCNNNNNNNGC 1 cut(s) 675
HpyF3I CTNAG 6 cut(s) 9, 17, 127, 269, 412, 853
HpySE526I ACGT 3 cut(s) 152, 647, 840
Hsp92II CATG 9 cut(s) 26, 90, 311, 358, 559, 826, 918, 929, 1014
LmnI GCTCC 1 cut(s) 1106
Lsp1109I GCAGC 3 cut(s) 665, 814, 1051
MaeI CTAG 4 cut(s) 69, 375, 596, 806
MaeII ACGT 3 cut(s) 152, 647, 840
MaeIII GTNAC 3 cut(s) 258, 542, 950
MboII GAAGA 7 cut(s) 438, 494, 654, 809, 982, 985, 1158
MflI RGATCY 3 cut(s) 654, 745, 814
MhlI GDGCHC 1 cut(s) 721
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 8 cut(s) 231, 316, 407, 435, 767, 940, 1036, 1089
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 2 cut(s) 113, 278
MmeI TCCRAC 3 cut(s) 271, 1046, 1108
MnlI CCTC 7 cut(s) 249, 273, 632, 878, 923, 985, 1054
Mox20I TGGCCA 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 199
MscI TGGCCA 1 cut(s) 522
MseI TTAA 3 cut(s) 552, 753, 1035
MslI CAYNNNNRTG 2 cut(s) 270, 839
Msp20I TGGCCA 1 cut(s) 522
MspI CCGG 2 cut(s) 98, 135
MspR9I CCNGG 1 cut(s) 136
MwoI GCNNNNNNNGC 1 cut(s) 675
NciI CCSGG 1 cut(s) 136
NcoI CCATGG 2 cut(s) 307, 822
NlaIII CATG 9 cut(s) 26, 90, 311, 358, 559, 826, 918, 929, 1014
NlaIV GGNNCC 3 cut(s) 656, 719, 1102
NspI RCATGY 2 cut(s) 26, 918
NspV TTCGAA 1 cut(s) 193
OliI CACNNNNGTG 1 cut(s) 839
PceI AGGCCT 1 cut(s) 578
PciI ACATGT 1 cut(s) 22
PdmI GAANNNNTTC 1 cut(s) 199
PflFI GACNNNGTC 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 1074
PkrI GCNGC 3 cut(s) 680, 829, 1066
PleI GAGTC 2 cut(s) 113, 278
PpsI GAGTC 2 cut(s) 113, 278
Ppu21I YACGTR 1 cut(s) 841
PscI ACATGT 1 cut(s) 22
PsiI TTATAA 1 cut(s) 230
Psp1406I AACGTT 2 cut(s) 152, 647
PspEI GGTNACC 1 cut(s) 258
PspN4I GGNNCC 3 cut(s) 656, 719, 1102
PspOMI GGGCCC 1 cut(s) 717
PspPI GGNCC 5 cut(s) 371, 668, 717, 718, 763
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 3 cut(s) 654, 745, 814
PsyI GACNNNGTC 1 cut(s) 375
RsaI GTAC 1 cut(s) 863
RsaNI GTAC 1 cut(s) 862
RseI CAYNNNNRTG 2 cut(s) 270, 839
SaqAI TTAA 3 cut(s) 552, 753, 1035
SatI GCNGC 3 cut(s) 679, 828, 1065
Sau96I GGNCC 5 cut(s) 371, 668, 717, 718, 763
SchI GAGTC 2 cut(s) 113, 278
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 721
SfcI CTRYAG 1 cut(s) 327
SfuI TTCGAA 1 cut(s) 193
SinI GGWCC 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 270, 839
SmlI CTYRAG 2 cut(s) 253, 495
SmoI CTYRAG 2 cut(s) 253, 495
Sse9I AATT 8 cut(s) 231, 316, 407, 435, 767, 940, 1036, 1089
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 2 cut(s) 147, 442
SspMI CTAG 4 cut(s) 69, 375, 596, 806
StuI AGGCCT 1 cut(s) 578
StyD4I CCNGG 1 cut(s) 134
StyI CCWWGG 2 cut(s) 307, 822
TaaI ACNGT 1 cut(s) 950
TaiI ACGT 3 cut(s) 155, 650, 843
TaqI TCGA 3 cut(s) 193, 405, 981
TasI AATT 8 cut(s) 231, 316, 407, 435, 767, 940, 1036, 1089
TatI WGTACW 1 cut(s) 861
Tru1I TTAA 3 cut(s) 552, 753, 1035
Tru9I TTAA 3 cut(s) 552, 753, 1035
TscAI CASTG 2 cut(s) 541, 801
TseI GCWGC 3 cut(s) 678, 827, 1064
TspDTI ATGAA 2 cut(s) 229, 375
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 2 cut(s) 541, 801
Tth111I GACNNNGTC 1 cut(s) 375
Van91I CCANNNNNTGG 1 cut(s) 1074
VpaK11BI GGWCC 1 cut(s) 371
XapI RAATTY 3 cut(s) 316, 407, 435
XceI RCATGY 2 cut(s) 26, 918
XmiI GTMKAC 1 cut(s) 849
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 4 cut(s) 69, 375, 596, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.