Rw0G020090

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00906
OG0014950
Physical Location & Seq
Forward (+)
326 .. 784
459 bp
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UTR
Exon/CDS
Intron
Rw0G020090.1

Sequence Viewer

Length: 342 bp
ATGGCTGCATTCACCTATGTGGATATCTACTCACTAAAGTACACCCTCATTACTCAAGCCAAGAAATATGGATTTGAGAAGCCACTCGTAGCATGTTGTGGTCGTGGTGGGAAATACAATTACAACCTACATGAGAAGTGTGGGGCGAAGAAGACTATCAACGGCAAAGAGGTAGTAGTCGCTAACTCGTGCAAGGATCCAATGACTAGGATTAATTGGGACGGGACGCATTTCACTGAGGCTGCCAACAAATGGATATTTCAACAAATTTTGAATGGCTCCTTTTCAGATCCACCAAACCCGTTGGAAATGGCTTGTCAAAGAAGGAAAACAAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

113

Amino Acids

12.83

Weight (kDa)

9.44

Isoelectric Point (pI)

22.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 3 - 90 4.3e-07 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26430
fragaria_vesca FvH4_4g26020
malus_domestica MD16G1100400.v1.1
prunus_persica Prupe.1G246600_v2.0.a1 Prupe.1G246600_v2.0.a1
pyrus_communis pycom16g08510
rosa_chinensis RchiOBHm_Chr4g0433461
rosa_laevigata RLG00000006751
rosa_multiflora Rmu_sc0000312.1_g000001
rosa_roxburghii Rroxscaffold_5G00375000
rosa_rugosa Rorug04G0269000
rosa_samantha Rh4BG331600 Rh4CG346900 Rh4DG327900
rosa_wichuraiana Rw0G020090 Rw4G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 252
AclWI GGATC 3 cut(s) 191, 204, 284
AcsI RAATTY 1 cut(s) 267
AfaI GTAC 1 cut(s) 41
AfiI CCNNNNNNNGG 1 cut(s) 252
AgsI TTSAA 2 cut(s) 263, 274
AleI CACNNNNGTG 1 cut(s) 17
AlwI GGATC 3 cut(s) 191, 204, 284
ApeKI GCWGC 2 cut(s) 5, 242
ApoI RAATTY 1 cut(s) 267
AseI ATTAAT 1 cut(s) 213
AsuHPI GGTGA 1 cut(s) 4
BamHI GGATCC 1 cut(s) 196
BauI CACGAG 1 cut(s) 187
BbsI GAAGAC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 229
BceAI ACGGC 1 cut(s) 178
BfaI CTAG 1 cut(s) 207
BisI GCNGC 2 cut(s) 6, 243
BlsI GCNGC 2 cut(s) 7, 244
BmiI GGNNCC 2 cut(s) 198, 280
BpiI GAAGAC 1 cut(s) 158
BpuEI CTTGAG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 252
BseLI CCNNNNNNNGG 1 cut(s) 252
BseMII CTCAG 1 cut(s) 228
BseXI GCAGC 1 cut(s) 229
BslFI GGGAC 2 cut(s) 233, 238
BslI CCNNNNNNNGG 1 cut(s) 252
BsmFI GGGAC 2 cut(s) 233, 238
BsmI GAATGC 1 cut(s) 8
Bsp143I GATC 2 cut(s) 196, 289
BspCNI CTCAG 1 cut(s) 229
BspLI GGNNCC 2 cut(s) 198, 280
BspPI GGATC 3 cut(s) 191, 204, 284
BssMI GATC 2 cut(s) 196, 289
BssSI CACGAG 1 cut(s) 187
Bst2BI CACGAG 1 cut(s) 187
BstDEI CTNAG 1 cut(s) 237
BstKTI GATC 2 cut(s) 199, 292
BstMBI GATC 2 cut(s) 196, 289
BstNSI RCATGY 1 cut(s) 96
BstV1I GCAGC 1 cut(s) 229
BstV2I GAAGAC 1 cut(s) 158
BstX2I RGATCY 2 cut(s) 196, 289
BstYI RGATCY 2 cut(s) 196, 289
BtsIMutI CAGTG 1 cut(s) 234
CseI GACGC 1 cut(s) 235
Csp6I GTAC 1 cut(s) 40
CviAII CATG 2 cut(s) 93, 131
CviJI RGCY 6 cut(s) 5, 59, 82, 242, 279, 314
CviKI_1 RGCY 6 cut(s) 5, 59, 82, 242, 279, 314
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 1 cut(s) 237
DpnI GATC 2 cut(s) 198, 291
DpnII GATC 2 cut(s) 196, 289
Eco32I GATATC 1 cut(s) 25
EcoRV GATATC 1 cut(s) 25
FaeI CATG 2 cut(s) 96, 134
FaiI YATR 4 cut(s) 18, 69, 94, 132
FaqI GGGAC 2 cut(s) 233, 238
FatI CATG 2 cut(s) 92, 130
Fnu4HI GCNGC 2 cut(s) 6, 243
Fsp4HI GCNGC 2 cut(s) 6, 243
FspBI CTAG 1 cut(s) 207
GluI GCNGC 2 cut(s) 6, 243
HgaI GACGC 1 cut(s) 235
Hin1II CATG 2 cut(s) 96, 134
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 1 cut(s) 42
Hpy188I TCNGA 1 cut(s) 289
Hpy8I GTNNAC 1 cut(s) 42
HpyAV CCTTC 1 cut(s) 318
HpyCH4V TGCA 2 cut(s) 8, 192
HpyF3I CTNAG 1 cut(s) 237
Hsp92II CATG 2 cut(s) 96, 134
Kzo9I GATC 2 cut(s) 196, 289
LmnI GCTCC 1 cut(s) 284
Lsp1109I GCAGC 1 cut(s) 229
MaeI CTAG 1 cut(s) 207
MalI GATC 2 cut(s) 198, 291
MboI GATC 2 cut(s) 196, 289
MboII GAAGA 2 cut(s) 160, 163
MflI RGATCY 2 cut(s) 196, 289
MluCI AATT 3 cut(s) 118, 214, 267
MmeI TCCRAC 1 cut(s) 285
MnlI CCTC 3 cut(s) 56, 163, 232
MseI TTAA 1 cut(s) 213
MslI CAYNNNNRTG 1 cut(s) 17
Mva1269I GAATGC 1 cut(s) 8
NdeII GATC 2 cut(s) 196, 289
NlaIII CATG 2 cut(s) 96, 134
NlaIV GGNNCC 2 cut(s) 198, 280
NspI RCATGY 1 cut(s) 96
OliI CACNNNNGTG 1 cut(s) 17
PctI GAATGC 1 cut(s) 8
PflMI CCANNNNNTGG 1 cut(s) 252
PkrI GCNGC 2 cut(s) 7, 244
PshBI ATTAAT 1 cut(s) 213
PspN4I GGNNCC 2 cut(s) 198, 280
PsuI RGATCY 2 cut(s) 196, 289
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
RseI CAYNNNNRTG 1 cut(s) 17
SaqAI TTAA 1 cut(s) 213
SatI GCNGC 2 cut(s) 6, 243
Sau3AI GATC 2 cut(s) 196, 289
SetI ASST 3 cut(s) 17, 129, 174
SmiMI CAYNNNNRTG 1 cut(s) 17
SmlI CTYRAG 1 cut(s) 54
SmoI CTYRAG 1 cut(s) 54
Sse9I AATT 3 cut(s) 118, 214, 267
SspMI CTAG 1 cut(s) 207
TasI AATT 3 cut(s) 118, 214, 267
TatI WGTACW 1 cut(s) 39
Tru1I TTAA 1 cut(s) 213
Tru9I TTAA 1 cut(s) 213
TscAI CASTG 1 cut(s) 241
TseI GCWGC 2 cut(s) 5, 242
TspRI CASTG 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 252
VspI ATTAAT 1 cut(s) 213
XapI RAATTY 1 cut(s) 267
XceI RCATGY 1 cut(s) 96
XspI CTAG 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.