Rroxscaffold_5G00375000

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0014950
Physical Location & Seq
Forward (+)
55894075 .. 55904856
10782 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00375000.1

Sequence Viewer

Length: 609 bp
ATGACCATAGAACAAATAAAGGCCTATGTTCCGGATGTGCTAGGCCAGTTATCTAATGTCATCAAGAATATATACAATGAAGGGGGAAGAACATTTTGGATCCACAACACAGGCCCAGTGGGTTGCTACCCCTATATTCTGGATCGGTTCTTTGTCAACCCGGCCCAATATAATAAGTACGGGTGTGCAAGTCCGTTTAATGATGTGGCCCAATTTTTCAACCAAAGATTAAAGGAAGCTGTGGTTCAGCTTGAGAAAGATCTTCCCATGGCTGCATTCACCTATGTGGATATCTACTCACTAAAGTACACACTCATTACTCAAGCCAAGAAATATGGATTTGAGAAGCCACTCGTAGCATGTTGTGGTCGTGGTGGGAAATACAATTACAACCTACATGAGAAGTGCGGGGCGAAGAAGACTATCAACGGCAAAGAGGTAGTAATCGCTAACTCGTGCAAGGATCCAATGACTAGGATTAATTGGGACGGGACGCATTTCACTGAGGCTGCCAACAAATGGATATTCCAACAAATTTTGAATGGCTCCTTTTCAGATCCACCAAACCCGTTGGAAATGGCTTGTCAAAGAAGGAAAACAAACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

202

Amino Acids

23.03

Weight (kDa)

9.08

Isoelectric Point (pI)

30.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 4 - 179 5.3e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26430
fragaria_vesca FvH4_4g26020
malus_domestica MD16G1100400.v1.1
prunus_persica Prupe.1G246600_v2.0.a1 Prupe.1G246600_v2.0.a1
pyrus_communis pycom16g08510
rosa_chinensis RchiOBHm_Chr4g0433461
rosa_laevigata RLG00000006751
rosa_multiflora Rmu_sc0000312.1_g000001
rosa_roxburghii Rroxscaffold_5G00375000
rosa_rugosa Rorug04G0269000
rosa_samantha Rh4BG331600 Rh4CG346900 Rh4DG327900
rosa_wichuraiana Rw0G020090 Rw4G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 519
AccIII TCCGGA 1 cut(s) 31
AciI CCGC 1 cut(s) 408
AclWI GGATC 6 cut(s) 94, 107, 150, 458, 471, 551
AcsI RAATTY 1 cut(s) 534
AfaI GTAC 2 cut(s) 179, 308
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 2 cut(s) 220, 541
AjuI GAANNNNNNNTTGG 2 cut(s) 79, 111
AleI CACNNNNGTG 1 cut(s) 284
AluBI AGCT 2 cut(s) 239, 250
AluI AGCT 2 cut(s) 239, 250
AlwI GGATC 6 cut(s) 94, 107, 150, 458, 471, 551
Aor13HI TCCGGA 1 cut(s) 31
AoxI GGCC 5 cut(s) 21, 43, 112, 162, 207
ApeKI GCWGC 2 cut(s) 272, 509
ApoI RAATTY 1 cut(s) 534
AseI ATTAAT 1 cut(s) 480
AspS9I GGNCC 3 cut(s) 113, 163, 208
AsuC2I CCSGG 1 cut(s) 161
AsuHPI GGTGA 1 cut(s) 271
BamHI GGATCC 2 cut(s) 99, 463
BauI CACGAG 1 cut(s) 454
BbsI GAAGAC 1 cut(s) 425
BbvI GCAGC 2 cut(s) 259, 496
BceAI ACGGC 1 cut(s) 445
BcnI CCSGG 1 cut(s) 161
BfaI CTAG 2 cut(s) 41, 474
BglII AGATCT 1 cut(s) 259
BisI GCNGC 2 cut(s) 273, 510
BlsI GCNGC 2 cut(s) 274, 511
Bme1390I CCNGG 1 cut(s) 161
BmgT120I GGNCC 3 cut(s) 113, 163, 208
BmiI GGNNCC 3 cut(s) 101, 465, 547
BmrFI CCNGG 1 cut(s) 161
BmrI ACTGGG 1 cut(s) 110
BmuI ACTGGG 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 425
BpuEI CTTGAG 2 cut(s) 272, 306
BpuMI CCSGG 1 cut(s) 161
BsaJI CCNNGG 1 cut(s) 267
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 2 cut(s) 46, 116
BseAI TCCGGA 1 cut(s) 31
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMII CTCAG 1 cut(s) 495
BseNI ACTGG 2 cut(s) 46, 116
BseXI GCAGC 2 cut(s) 259, 496
BshFI GGCC 5 cut(s) 23, 45, 114, 164, 209
BsiSI CCGG 2 cut(s) 32, 161
BslFI GGGAC 2 cut(s) 500, 505
BslI CCNNNNNNNGG 1 cut(s) 519
BsmFI GGGAC 2 cut(s) 500, 505
BsmI GAATGC 1 cut(s) 275
BsnI GGCC 5 cut(s) 23, 45, 114, 164, 209
Bsp13I TCCGGA 1 cut(s) 31
Bsp143I GATC 5 cut(s) 99, 142, 259, 463, 556
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 1 cut(s) 408
BspANI GGCC 5 cut(s) 23, 45, 114, 164, 209
BspCNI CTCAG 1 cut(s) 496
BspEI TCCGGA 1 cut(s) 31
BspLI GGNNCC 3 cut(s) 101, 465, 547
BspPI GGATC 6 cut(s) 94, 107, 150, 458, 471, 551
BsrI ACTGG 2 cut(s) 46, 116
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 5 cut(s) 99, 142, 259, 463, 556
BssSI CACGAG 1 cut(s) 454
BssT1I CCWWGG 1 cut(s) 267
Bst2BI CACGAG 1 cut(s) 454
BstDEI CTNAG 1 cut(s) 504
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 40
BstKTI GATC 5 cut(s) 102, 145, 262, 466, 559
BstMBI GATC 5 cut(s) 99, 142, 259, 463, 556
BstNSI RCATGY 1 cut(s) 363
BstSCI CCNGG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 259, 496
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 4 cut(s) 99, 259, 463, 556
BstYI RGATCY 4 cut(s) 99, 259, 463, 556
BsuRI GGCC 5 cut(s) 23, 45, 114, 164, 209
BtgI CCRYGG 1 cut(s) 267
BtsCI GGATG 1 cut(s) 40
BtsIMutI CAGTG 2 cut(s) 123, 501
Cfr13I GGNCC 3 cut(s) 113, 163, 208
CseI GACGC 1 cut(s) 502
Csp6I GTAC 2 cut(s) 178, 307
CviAII CATG 3 cut(s) 268, 360, 398
CviQI GTAC 2 cut(s) 178, 307
DdeI CTNAG 1 cut(s) 504
DpnI GATC 5 cut(s) 101, 144, 261, 465, 558
DpnII GATC 5 cut(s) 99, 142, 259, 463, 556
Eco130I CCWWGG 1 cut(s) 267
Eco147I AGGCCT 1 cut(s) 23
Eco32I GATATC 1 cut(s) 292
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 3 cut(s) 271, 363, 401
FaqI GGGAC 2 cut(s) 500, 505
FatI CATG 3 cut(s) 267, 359, 397
FauI CCCGC 1 cut(s) 401
Fnu4HI GCNGC 2 cut(s) 273, 510
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 2 cut(s) 273, 510
FspBI CTAG 2 cut(s) 41, 474
GluI GCNGC 2 cut(s) 273, 510
HaeIII GGCC 5 cut(s) 23, 45, 114, 164, 209
HapII CCGG 2 cut(s) 32, 161
HgaI GACGC 1 cut(s) 502
Hin1II CATG 3 cut(s) 271, 363, 401
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HpaII CCGG 2 cut(s) 32, 161
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 2 cut(s) 157, 309
Hpy188I TCNGA 1 cut(s) 556
Hpy188III TCNNGA 3 cut(s) 32, 64, 140
Hpy8I GTNNAC 2 cut(s) 157, 309
HpyAV CCTTC 2 cut(s) 74, 585
HpyCH4V TGCA 3 cut(s) 188, 275, 459
HpyF3I CTNAG 1 cut(s) 504
Hsp92II CATG 3 cut(s) 271, 363, 401
Kpn2I TCCGGA 1 cut(s) 31
Kzo9I GATC 5 cut(s) 99, 142, 259, 463, 556
LmnI GCTCC 1 cut(s) 551
LpnPI CCDG 6 cut(s) 45, 59, 96, 125, 129, 174
Lsp1109I GCAGC 2 cut(s) 259, 496
MaeI CTAG 2 cut(s) 41, 474
MalI GATC 5 cut(s) 101, 144, 261, 465, 558
MboI GATC 5 cut(s) 99, 142, 259, 463, 556
MboII GAAGA 4 cut(s) 99, 254, 427, 430
MflI RGATCY 4 cut(s) 99, 259, 463, 556
MluCI AATT 4 cut(s) 212, 385, 481, 534
MmeI TCCRAC 2 cut(s) 552, 553
MnlI CCTC 2 cut(s) 430, 499
MroI TCCGGA 1 cut(s) 31
MseI TTAA 3 cut(s) 198, 230, 480
MslI CAYNNNNRTG 1 cut(s) 284
MspI CCGG 2 cut(s) 32, 161
MspR9I CCNGG 1 cut(s) 161
Mva1269I GAATGC 1 cut(s) 275
NciI CCSGG 1 cut(s) 161
NcoI CCATGG 1 cut(s) 267
NdeII GATC 5 cut(s) 99, 142, 259, 463, 556
NlaIII CATG 3 cut(s) 271, 363, 401
NlaIV GGNNCC 3 cut(s) 101, 465, 547
NspI RCATGY 1 cut(s) 363
OliI CACNNNNGTG 1 cut(s) 284
PceI AGGCCT 1 cut(s) 23
PctI GAATGC 1 cut(s) 275
PflMI CCANNNNNTGG 1 cut(s) 519
PkrI GCNGC 2 cut(s) 274, 511
PshBI ATTAAT 1 cut(s) 480
PspN4I GGNNCC 3 cut(s) 101, 465, 547
PspPI GGNCC 3 cut(s) 113, 163, 208
PsuI RGATCY 4 cut(s) 99, 259, 463, 556
RsaI GTAC 2 cut(s) 179, 308
RsaNI GTAC 2 cut(s) 178, 307
RseI CAYNNNNRTG 1 cut(s) 284
SaqAI TTAA 3 cut(s) 198, 230, 480
SatI GCNGC 2 cut(s) 273, 510
Sau3AI GATC 5 cut(s) 99, 142, 259, 463, 556
Sau96I GGNCC 3 cut(s) 113, 163, 208
ScrFI CCNGG 1 cut(s) 161
SetI ASST 5 cut(s) 241, 252, 284, 396, 441
SmiMI CAYNNNNRTG 1 cut(s) 284
SmlI CTYRAG 2 cut(s) 251, 321
SmoI CTYRAG 2 cut(s) 251, 321
Sse9I AATT 4 cut(s) 212, 385, 481, 534
SseBI AGGCCT 1 cut(s) 23
SsiI CCGC 1 cut(s) 408
SspMI CTAG 2 cut(s) 41, 474
StuI AGGCCT 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 159
StyI CCWWGG 1 cut(s) 267
TasI AATT 4 cut(s) 212, 385, 481, 534
TatI WGTACW 1 cut(s) 306
Tru1I TTAA 3 cut(s) 198, 230, 480
Tru9I TTAA 3 cut(s) 198, 230, 480
TscAI CASTG 2 cut(s) 123, 508
TseI GCWGC 2 cut(s) 272, 509
TspDTI ATGAA 1 cut(s) 93
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 2 cut(s) 123, 508
Van91I CCANNNNNTGG 1 cut(s) 519
VspI ATTAAT 1 cut(s) 480
XapI RAATTY 1 cut(s) 534
XceI RCATGY 1 cut(s) 363
XspI CTAG 2 cut(s) 41, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.