Rh4BG331600

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0014950
Physical Location & Seq
Forward (+)
49625661 .. 49627886
2226 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG331600.1

Sequence Viewer

Length: 1164 bp
ATGGAAACTCGGTTTCTAAGACATGTTGCCTTTGGGCTTGTGATTTGGGCAACAATGCTTCTGAGTCCTAGCACTTGTTCAAAACCATGTTCTTTTCCGGCCATATTCAACTTTGGAGACTCAAACTCAGACACCGGGGGCCTGTCCGCAGCGTTTGGACAAGCTCCTTTTCCCAATGGAGAGACATTTTTTGGAACACCTTCGGGACGTTATTCCGATGGCCGTCTTATAATTGATTTCATAGCTGAAAGCTTGAGATTACCTCATCTGAGTGCATTTTTGGACTCGGTTGGATCAAACTTCAGCCATGGAGCGAATTTTGCTACTGCAGGATCAACCATTAGACCCCCAAACATGACCATCCAATATGGACCTAGTCCCATTTCCTTAGATATGCAGTATGTTGAATTCTCAAACTTCCATATGAGATCGCAAATTTATCGCAAAAAAGGAGAGTTATTTGAGAAACTGCTTCCCAGAGAAGAGTACTTTTCTCAAGCACTTTATACCTTTGACATTGGCCAAAATGATCTCACTGCTGGTTACAAGCTTAACATGACCATAGAACAAATAAAGGCCTATGTTCCAGATGTGCTAGGCCAGTTCTCTAATGTCATCAAGAATGTATACAATGAAGGGGGAAGAACATTTTGGATCCACAACACAGGCCCAGTGGGCTGCTACCCTTATATTCTGGATCGGTTCTTTGTCAACCGGGCCCAATATAATAAGTACGGGTGTGCAAGTCCGTTTAATGATGTGGCCCAATTTTTCAACCAAAGATTAAAGGAAGCTGTGGTTCAGCTTGAGAAAGATCTTCCCATGGCTGCATTCACCTATGTGGATATCTACTCACTAAAGTACACCCTCATTACTCAAGCCAAGAAATATGGATTTGAGAAGCCACTCGTAGCATGTTGTGGTCGTGGTGGGAAATACAATTACAACCTACATGAGAAGTGTGGGGCGAAGAAGACTATCAACGGCAAAGAGGTAGTAATCGCTAACTCGTGCAAGGATCCAATGACTAGGATTAATTGGGACGGGACGCATTTCACTGAGGCTGCCAACAAATGGATATTCCAACAAATTTTGAATGGCTCCTTTTCAGATCCATCAAACCCGTTGGAAATGGCTTGTCAAAGAAGGAAAACAAAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

387

Amino Acids

43.61

Weight (kDa)

8.89

Isoelectric Point (pI)

25.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 35 - 364 5.9e-45 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26430
fragaria_vesca FvH4_4g26020
malus_domestica MD16G1100400.v1.1
prunus_persica Prupe.1G246600_v2.0.a1 Prupe.1G246600_v2.0.a1
pyrus_communis pycom16g08510
rosa_chinensis RchiOBHm_Chr4g0433461
rosa_laevigata RLG00000006751
rosa_multiflora Rmu_sc0000312.1_g000001
rosa_roxburghii Rroxscaffold_5G00375000
rosa_rugosa Rorug04G0269000
rosa_samantha Rh4BG331600 Rh4CG346900 Rh4DG327900
rosa_wichuraiana Rw0G020090 Rw4G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 230
AccB7I CCANNNNNTGG 1 cut(s) 1074
AccI GTMKAC 1 cut(s) 627
AciI CCGC 1 cut(s) 147
AclWI GGATC 8 cut(s) 301, 340, 649, 662, 705, 1013, 1026, 1106
AcoI YGGCCR 3 cut(s) 99, 220, 520
AcsI RAATTY 4 cut(s) 316, 407, 435, 1089
AcuI CTGAAG 1 cut(s) 286
AfaI GTAC 3 cut(s) 488, 734, 863
AfiI CCNNNNNNNGG 1 cut(s) 1074
AflIII ACRYGT 1 cut(s) 22
AgsI TTSAA 5 cut(s) 81, 109, 407, 775, 1096
AjuI GAANNNNNNNTTGG 2 cut(s) 634, 666
AleI CACNNNNGTG 1 cut(s) 839
AluBI AGCT 6 cut(s) 164, 245, 252, 550, 794, 805
AluI AGCT 6 cut(s) 164, 245, 252, 550, 794, 805
Alw26I GTCTC 2 cut(s) 111, 176
AlwI GGATC 8 cut(s) 301, 340, 649, 662, 705, 1013, 1026, 1106
AoxI GGCC 9 cut(s) 99, 139, 220, 520, 576, 598, 667, 717, 762
ApaI GGGCCC 1 cut(s) 721
ApeKI GCWGC 4 cut(s) 149, 678, 827, 1064
ApoI RAATTY 4 cut(s) 316, 407, 435, 1089
AseI ATTAAT 1 cut(s) 1035
Asp700I GAANNNNTTC 1 cut(s) 199
AspS9I GGNCC 6 cut(s) 139, 371, 668, 717, 718, 763
AsuC2I CCSGG 2 cut(s) 136, 716
AsuHPI GGTGA 1 cut(s) 826
AvaII GGWCC 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 721
BalI TGGCCA 1 cut(s) 522
BamHI GGATCC 2 cut(s) 654, 1018
BanII GRGCYC 1 cut(s) 721
BauI CACGAG 1 cut(s) 1009
BbsI GAAGAC 1 cut(s) 980
BbvI GCAGC 4 cut(s) 161, 665, 814, 1051
BccI CCATC 3 cut(s) 212, 368, 1123
BceAI ACGGC 2 cut(s) 207, 1000
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BcnI CCSGG 2 cut(s) 136, 716
BcoDI GTCTC 2 cut(s) 111, 176
BfaI CTAG 4 cut(s) 69, 375, 596, 1029
BfmI CTRYAG 1 cut(s) 327
BglI GCCNNNNNGGC 1 cut(s) 675
BglII AGATCT 1 cut(s) 814
BisI GCNGC 4 cut(s) 150, 679, 828, 1065
BlsI GCNGC 4 cut(s) 151, 680, 829, 1066
BmcAI AGTACT 1 cut(s) 488
Bme1390I CCNGG 2 cut(s) 136, 716
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 6 cut(s) 139, 371, 668, 717, 718, 763
BmiI GGNNCC 5 cut(s) 140, 656, 719, 1020, 1102
BmrFI CCNGG 2 cut(s) 136, 716
BmrI ACTGGG 1 cut(s) 665
BmuI ACTGGG 1 cut(s) 665
BpiI GAAGAC 1 cut(s) 980
BplI GAGNNNNNCTC 2 cut(s) 247, 279
BpuEI CTTGAG 4 cut(s) 274, 480, 827, 861
BpuMI CCSGG 2 cut(s) 136, 716
BsaJI CCNNGG 3 cut(s) 135, 307, 822
Bsc4I CCNNNNNNNGG 1 cut(s) 1074
Bse1I ACTGG 2 cut(s) 601, 671
BseDI CCNNGG 3 cut(s) 135, 307, 822
BseGI GGATG 1 cut(s) 360
BseLI CCNNNNNNNGG 1 cut(s) 1074
BseMII CTCAG 4 cut(s) 53, 141, 260, 1050
BseNI ACTGG 2 cut(s) 601, 671
BseSI GKGCMC 1 cut(s) 721
BseXI GCAGC 4 cut(s) 161, 665, 814, 1051
BshFI GGCC 9 cut(s) 101, 141, 222, 522, 578, 600, 669, 719, 764
BsiSI CCGG 3 cut(s) 98, 135, 715
BslFI GGGAC 4 cut(s) 219, 363, 1055, 1060
BslI CCNNNNNNNGG 1 cut(s) 1074
BsmAI GTCTC 2 cut(s) 111, 176
BsmFI GGGAC 4 cut(s) 219, 363, 1055, 1060
BsmI GAATGC 1 cut(s) 830
BsnI GGCC 9 cut(s) 101, 141, 222, 522, 578, 600, 669, 719, 764
Bsp120I GGGCCC 1 cut(s) 717
Bsp1286I GDGCHC 1 cut(s) 721
Bsp143I GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
Bsp19I CCATGG 2 cut(s) 307, 822
BspACI CCGC 1 cut(s) 147
BspANI GGCC 9 cut(s) 101, 141, 222, 522, 578, 600, 669, 719, 764
BspCNI CTCAG 4 cut(s) 54, 140, 261, 1051
BspLI GGNNCC 5 cut(s) 140, 656, 719, 1020, 1102
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 8 cut(s) 301, 340, 649, 662, 705, 1013, 1026, 1106
BsrI ACTGG 2 cut(s) 601, 671
BssECI CCNNGG 3 cut(s) 135, 307, 822
BssMI GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
BssNAI GTATAC 1 cut(s) 628
BssSI CACGAG 1 cut(s) 1009
BssT1I CCWWGG 2 cut(s) 307, 822
Bst1107I GTATAC 1 cut(s) 628
Bst2BI CACGAG 1 cut(s) 1009
Bst6I CTCTTC 1 cut(s) 477
BstDEI CTNAG 6 cut(s) 17, 62, 127, 269, 388, 1059
BstDSI CCRYGG 2 cut(s) 307, 822
BstF5I GGATG 1 cut(s) 360
BstKTI GATC 9 cut(s) 296, 335, 431, 532, 657, 700, 817, 1021, 1114
BstMAI GTCTC 2 cut(s) 111, 176
BstMBI GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
BstMWI GCNNNNNNNGC 2 cut(s) 320, 675
BstNSI RCATGY 2 cut(s) 26, 918
BstSCI CCNGG 2 cut(s) 134, 714
BstSFI CTRYAG 1 cut(s) 327
BstSLI GKGCMC 1 cut(s) 721
BstV1I GCAGC 4 cut(s) 161, 665, 814, 1051
BstV2I GAAGAC 1 cut(s) 980
BstX2I RGATCY 4 cut(s) 654, 814, 1018, 1111
BstYI RGATCY 4 cut(s) 654, 814, 1018, 1111
BstZ17I GTATAC 1 cut(s) 628
BsuRI GGCC 9 cut(s) 101, 141, 222, 522, 578, 600, 669, 719, 764
BtgI CCRYGG 2 cut(s) 307, 822
BtsCI GGATG 1 cut(s) 360
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 3 cut(s) 534, 678, 1056
Cfr13I GGNCC 6 cut(s) 139, 371, 668, 717, 718, 763
CseI GACGC 1 cut(s) 1057
Csp6I GTAC 3 cut(s) 487, 733, 862
CviAII CATG 8 cut(s) 23, 87, 308, 355, 556, 823, 915, 953
CviQI GTAC 3 cut(s) 487, 733, 862
DdeI CTNAG 6 cut(s) 17, 62, 127, 269, 388, 1059
DpnI GATC 9 cut(s) 295, 334, 430, 531, 656, 699, 816, 1020, 1113
DpnII GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
EaeI YGGCCR 3 cut(s) 99, 220, 520
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Eco130I CCWWGG 2 cut(s) 307, 822
Eco147I AGGCCT 1 cut(s) 578
Eco24I GRGCYC 1 cut(s) 721
Eco32I GATATC 1 cut(s) 847
Eco47I GGWCC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 286
EcoO109I RGGNCCY 1 cut(s) 139
EcoRI GAATTC 1 cut(s) 407
EcoRV GATATC 1 cut(s) 847
EcoT14I CCWWGG 2 cut(s) 307, 822
EcoT38I GRGCYC 1 cut(s) 721
ErhI CCWWGG 2 cut(s) 307, 822
FaeI CATG 8 cut(s) 26, 90, 311, 358, 559, 826, 918, 956
FaqI GGGAC 4 cut(s) 219, 363, 1055, 1060
FatI CATG 8 cut(s) 22, 86, 307, 354, 555, 822, 914, 952
FauNDI CATATG 1 cut(s) 423
FblI GTMKAC 1 cut(s) 627
Fnu4HI GCNGC 4 cut(s) 150, 679, 828, 1065
FokI GGATG 1 cut(s) 347
FriOI GRGCYC 1 cut(s) 721
Fsp4HI GCNGC 4 cut(s) 150, 679, 828, 1065
FspBI CTAG 4 cut(s) 69, 375, 596, 1029
GluI GCNGC 4 cut(s) 150, 679, 828, 1065
HaeIII GGCC 9 cut(s) 101, 141, 222, 522, 578, 600, 669, 719, 764
HapII CCGG 3 cut(s) 98, 135, 715
HgaI GACGC 1 cut(s) 1057
Hin1II CATG 8 cut(s) 26, 90, 311, 358, 559, 826, 918, 956
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HindIII AAGCTT 2 cut(s) 250, 548
HinfI GANTC 3 cut(s) 64, 119, 284
HpaII CCGG 3 cut(s) 98, 135, 715
HphI GGTGA 1 cut(s) 826
Hpy166II GTNNAC 3 cut(s) 628, 712, 864
Hpy188I TCNGA 5 cut(s) 63, 130, 217, 270, 1111
Hpy188III TCNNGA 4 cut(s) 204, 587, 619, 695
Hpy8I GTNNAC 3 cut(s) 628, 712, 864
HpyAV CCTTC 3 cut(s) 210, 629, 1140
HpyCH4IV ACGT 1 cut(s) 208
HpyCH4V TGCA 6 cut(s) 275, 329, 397, 743, 830, 1014
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 675
HpyF3I CTNAG 6 cut(s) 17, 62, 127, 269, 388, 1059
HpySE526I ACGT 1 cut(s) 208
Hsp92II CATG 8 cut(s) 26, 90, 311, 358, 559, 826, 918, 956
Kzo9I GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
LmnI GCTCC 3 cut(s) 169, 311, 1106
Lsp1109I GCAGC 4 cut(s) 161, 665, 814, 1051
MaeI CTAG 4 cut(s) 69, 375, 596, 1029
MaeII ACGT 1 cut(s) 208
MaeIII GTNAC 1 cut(s) 542
MalI GATC 9 cut(s) 295, 334, 430, 531, 656, 699, 816, 1020, 1113
MboI GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
MboII GAAGA 5 cut(s) 494, 654, 809, 982, 985
MflI RGATCY 4 cut(s) 654, 814, 1018, 1111
MhlI GDGCHC 1 cut(s) 721
MlsI TGGCCA 1 cut(s) 522
MluCI AATT 8 cut(s) 231, 316, 407, 435, 767, 940, 1036, 1089
MluNI TGGCCA 1 cut(s) 522
MlyI GAGTC 3 cut(s) 73, 113, 278
MmeI TCCRAC 3 cut(s) 271, 1107, 1108
MnlI CCTC 4 cut(s) 273, 878, 985, 1054
Mox20I TGGCCA 1 cut(s) 522
MroXI GAANNNNTTC 1 cut(s) 199
MscI TGGCCA 1 cut(s) 522
MseI TTAA 4 cut(s) 552, 753, 785, 1035
MslI CAYNNNNRTG 2 cut(s) 270, 839
Msp20I TGGCCA 1 cut(s) 522
MspI CCGG 3 cut(s) 98, 135, 715
MspR9I CCNGG 2 cut(s) 136, 716
Mva1269I GAATGC 1 cut(s) 830
MwoI GCNNNNNNNGC 2 cut(s) 320, 675
NciI CCSGG 2 cut(s) 136, 716
NcoI CCATGG 2 cut(s) 307, 822
NdeI CATATG 1 cut(s) 423
NdeII GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
NlaIII CATG 8 cut(s) 26, 90, 311, 358, 559, 826, 918, 956
NlaIV GGNNCC 5 cut(s) 140, 656, 719, 1020, 1102
NspI RCATGY 2 cut(s) 26, 918
OliI CACNNNNGTG 1 cut(s) 839
PceI AGGCCT 1 cut(s) 578
PciI ACATGT 1 cut(s) 22
PctI GAATGC 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 199
PflFI GACNNNGTC 1 cut(s) 375
PflMI CCANNNNNTGG 1 cut(s) 1074
PkrI GCNGC 4 cut(s) 151, 680, 829, 1066
PleI GAGTC 3 cut(s) 72, 113, 278
PpsI GAGTC 3 cut(s) 72, 113, 278
PscI ACATGT 1 cut(s) 22
PshBI ATTAAT 1 cut(s) 1035
PsiI TTATAA 1 cut(s) 230
PspN4I GGNNCC 5 cut(s) 140, 656, 719, 1020, 1102
PspOMI GGGCCC 1 cut(s) 717
PspPI GGNCC 6 cut(s) 139, 371, 668, 717, 718, 763
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 4 cut(s) 654, 814, 1018, 1111
PsyI GACNNNGTC 1 cut(s) 375
RsaI GTAC 3 cut(s) 488, 734, 863
RsaNI GTAC 3 cut(s) 487, 733, 862
RseI CAYNNNNRTG 2 cut(s) 270, 839
SaqAI TTAA 4 cut(s) 552, 753, 785, 1035
SatI GCNGC 4 cut(s) 150, 679, 828, 1065
Sau3AI GATC 9 cut(s) 293, 332, 428, 529, 654, 697, 814, 1018, 1111
Sau96I GGNCC 6 cut(s) 139, 371, 668, 717, 718, 763
ScaI AGTACT 1 cut(s) 488
SchI GAGTC 3 cut(s) 73, 113, 278
ScrFI CCNGG 2 cut(s) 136, 716
SduI GDGCHC 1 cut(s) 721
SfcI CTRYAG 1 cut(s) 327
SinI GGWCC 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 270, 839
SmlI CTYRAG 4 cut(s) 253, 495, 806, 876
SmoI CTYRAG 4 cut(s) 253, 495, 806, 876
Sse9I AATT 8 cut(s) 231, 316, 407, 435, 767, 940, 1036, 1089
SseBI AGGCCT 1 cut(s) 578
SsiI CCGC 1 cut(s) 147
SspMI CTAG 4 cut(s) 69, 375, 596, 1029
StuI AGGCCT 1 cut(s) 578
StyD4I CCNGG 2 cut(s) 134, 714
StyI CCWWGG 2 cut(s) 307, 822
TaiI ACGT 1 cut(s) 211
TasI AATT 8 cut(s) 231, 316, 407, 435, 767, 940, 1036, 1089
TatI WGTACW 2 cut(s) 486, 861
Tru1I TTAA 4 cut(s) 552, 753, 785, 1035
Tru9I TTAA 4 cut(s) 552, 753, 785, 1035
TscAI CASTG 3 cut(s) 541, 678, 1063
TseI GCWGC 4 cut(s) 149, 678, 827, 1064
TspDTI ATGAA 2 cut(s) 229, 648
TspGWI ACGGA 1 cut(s) 738
TspRI CASTG 3 cut(s) 541, 678, 1063
Tth111I GACNNNGTC 1 cut(s) 375
Van91I CCANNNNNTGG 1 cut(s) 1074
VpaK11BI GGWCC 1 cut(s) 371
VspI ATTAAT 1 cut(s) 1035
XapI RAATTY 4 cut(s) 316, 407, 435, 1089
XceI RCATGY 2 cut(s) 26, 918
XmiI GTMKAC 1 cut(s) 627
XmnI GAANNNNTTC 1 cut(s) 199
XspI CTAG 4 cut(s) 69, 375, 596, 1029
ZrmI AGTACT 1 cut(s) 488
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.