MD16G1100400.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0014950
Physical Location & Seq
Forward (+)
6959637 .. 6962797
3161 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1100400.v1.1.491

Sequence Viewer

Length: 1335 bp
ATGCAAGTACTATCTTTCTTTTTAAATTATTTTTTCCAAGACCATGCTATCAAAGTGGTCAATGCGGTTAGCAAGGAAAAGATAAAGAAAGACCCTCTTTCGTCGTCTCTCTATAAAAACAAGGCCGTCTACTTGTCTACACTTCTCCCCTTGAGTCTCTCTCTATGCACTCAACACATGGAATTTGTTTTCTTAAAACATCATGTTCTCTTCGGCCTTGTAATTTGGGCAACACTGCTTCCGAGTCCCGGCATTTGCACAGGGCCATGCTCTTTTCCGGCCATATTCAATTTCGGAGATTCGAACTCGGACACCGGGGGTTTATCTGCAGCATTCGGACAAGCCCCTTACCCCAACGGAGAGACCTACTTTCACGTCCCGTCCGGCCGTTTCTCCGACGGCCGTCTTGTAATCGATTTCATAGCGGAAAGCTTGGGGTTGCCTCATCTGAGTGCATTTCTTGACTCGATGGGATCAAACTTCAGCCATGGAGCCAATTTTGCCACAGCTGGATCAACCGTTATACCTCCAAACACAACCATTTCCCAAGGTGGGGCGAGTCCCATCTCATTAGACGTGCAGCTTCTTCAATTCTCCGACTTCCGCACACGATCCCAAATTTATCGCACAAAAAGCGAATTATTCGAGAAATCGCTGCCGAAGGAAGAGTACTTTTCTCAAGCCCTATATACCTTTGACATCGGCCAAAATGATATCACTGCTGGCTACAAACTCAATTGGACCGCAGAACAAGTCAAGGCATATCTTCCATATGTACTCACCCAGCTCTCAAATGCCATCAAGAGTGTATATGATCAAGGAGGAAGATCGTTTTGGATACATAACACAGGCCCATTGGGCTGCCTCCCTTATGTTTTGGTCCGGTTCGTCACTGATCCGGCCCAAATAAATAAATATGGGTGTGCAGATCAGATCGATGAGGTGGCCAAATTTTTCAATCAAAGATTAAAGGAAGCTGTGGTTCAACTCAAGAAAGATCTTCCGTTGGCTGCAATCACCTATGTGGATGTCTACTCAGTAAAGTACACCCTCATCACCCAAGCCAAAAAATATGGTTTTGAGAATCCCCTCATCGCGTGTTGCGGTCACGGTGGTAAATATAACTTCGACCGGTACGCAAAGTGCGGGGCCAAGAAAATGATCAACGGCACGGAGACGTTGATAGCCAAATCATGCGATGATCCAACGGTTCGGATCAACTGGGACGGGACCCACTTCACGGAGGCAGCCAACAAGTGGATATTCCAACAAATTGTGAACGGCTCCTTTTCGGACCCACCAAACCCTTTGAAAACGGCCTGTCGTTGCGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

445

Amino Acids

49.18

Weight (kDa)

8.35

Isoelectric Point (pI)

25.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 95 - 425 1.3e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26430
fragaria_vesca FvH4_4g26020
malus_domestica MD16G1100400.v1.1
prunus_persica Prupe.1G246600_v2.0.a1 Prupe.1G246600_v2.0.a1
pyrus_communis pycom16g08510
rosa_chinensis RchiOBHm_Chr4g0433461
rosa_laevigata RLG00000006751
rosa_multiflora Rmu_sc0000312.1_g000001
rosa_roxburghii Rroxscaffold_5G00375000
rosa_rugosa Rorug04G0269000
rosa_samantha Rh4BG331600 Rh4CG346900 Rh4DG327900
rosa_wichuraiana Rw0G020090 Rw4G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1257
AccI GTMKAC 3 cut(s) 129, 137, 1032
AccII CGCG 1 cut(s) 1097
AciI CCGC 7 cut(s) 65, 425, 604, 744, 1104, 1146, 1329
AclWI GGATC 6 cut(s) 481, 520, 606, 890, 1196, 1223
AcoI YGGCCR 5 cut(s) 279, 385, 400, 703, 945
AcsI RAATTY 3 cut(s) 182, 618, 950
AcuI CTGAAG 1 cut(s) 466
AfaI GTAC 5 cut(s) 9, 671, 777, 1046, 1136
AfiI CCNNNNNNNGG 5 cut(s) 248, 552, 553, 1240, 1257
AgeI ACCGGT 1 cut(s) 1131
AgsI TTSAA 5 cut(s) 289, 590, 958, 986, 1312
AjiI CACGTC 2 cut(s) 376, 577
AjuI GAANNNNNNNTTGG 2 cut(s) 817, 849
AleI CACNNNNGTG 1 cut(s) 1022
AluBI AGCT 5 cut(s) 432, 509, 583, 787, 977
AluI AGCT 5 cut(s) 432, 509, 583, 787, 977
Alw26I GTCTC 4 cut(s) 111, 161, 356, 1169
AlwI GGATC 6 cut(s) 481, 520, 606, 890, 1196, 1223
ApeKI GCWGC 6 cut(s) 329, 580, 655, 861, 1010, 1247
ApoI RAATTY 3 cut(s) 182, 618, 950
AsiGI ACCGGT 1 cut(s) 1131
AspS9I GGNCC 8 cut(s) 263, 741, 851, 880, 901, 1149, 1230, 1294
AsuC2I CCSGG 2 cut(s) 249, 316
AsuHPI GGTGA 3 cut(s) 772, 1009, 1048
AsuII TTCGAA 1 cut(s) 302
AvaII GGWCC 4 cut(s) 741, 880, 1230, 1294
BalI TGGCCA 1 cut(s) 947
BarI GAAGNNNNNNTAC 2 cut(s) 653, 685
BbvI GCAGC 6 cut(s) 341, 592, 642, 848, 997, 1259
BccI CCATC 3 cut(s) 463, 572, 806
BceAI ACGGC 7 cut(s) 110, 372, 387, 415, 1183, 1297, 1332
BciVI GTATCC 1 cut(s) 831
BclI TGATCA 2 cut(s) 814, 1161
BcnI CCSGG 2 cut(s) 249, 316
BcoDI GTCTC 4 cut(s) 111, 161, 356, 1169
BfmI CTRYAG 1 cut(s) 327
BfuI GTATCC 1 cut(s) 831
BglI GCCNNNNNGGC 1 cut(s) 858
BglII AGATCT 1 cut(s) 997
BisI GCNGC 6 cut(s) 330, 581, 656, 862, 1011, 1248
BlsI GCNGC 6 cut(s) 331, 582, 657, 863, 1012, 1249
BmcAI AGTACT 2 cut(s) 9, 671
Bme1390I CCNGG 2 cut(s) 249, 316
Bme18I GGWCC 4 cut(s) 741, 880, 1230, 1294
BmgBI CACGTC 2 cut(s) 376, 577
BmgT120I GGNCC 8 cut(s) 263, 741, 851, 880, 901, 1149, 1230, 1294
BmiI GGNNCC 6 cut(s) 493, 1150, 1231, 1232, 1285, 1296
BmrFI CCNGG 2 cut(s) 249, 316
BmrI ACTGGG 1 cut(s) 1231
BmuI ACTGGG 1 cut(s) 1231
BoxI GACNNNNGTC 1 cut(s) 402
BplI GAGNNNNNCTC 2 cut(s) 145, 177
Bpu14I TTCGAA 1 cut(s) 302
BpuEI CTTGAG 3 cut(s) 172, 663, 974
BpuMI CCSGG 2 cut(s) 249, 316
Bsa29I ATCGAT 2 cut(s) 414, 936
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 3 cut(s) 315, 487, 547
BsaWI WCCGGW 2 cut(s) 882, 1131
Bsc4I CCNNNNNNNGG 5 cut(s) 248, 552, 553, 1240, 1257
Bse118I RCCGGY 1 cut(s) 1131
Bse1I ACTGG 1 cut(s) 1226
BseCI ATCGAT 2 cut(s) 414, 936
BseDI CCNNGG 3 cut(s) 315, 487, 547
BseGI GGATG 1 cut(s) 1033
BseLI CCNNNNNNNGG 5 cut(s) 248, 552, 553, 1240, 1257
BseMII CTCAG 2 cut(s) 440, 1050
BseNI ACTGG 1 cut(s) 1226
BseX3I CGGCCG 2 cut(s) 385, 400
BseXI GCAGC 6 cut(s) 341, 592, 642, 848, 997, 1259
BseYI CCCAGC 1 cut(s) 783
BsgI GTGCAG 2 cut(s) 599, 945
Bsh1236I CGCG 1 cut(s) 1097
Bsh1285I CGRYCG 3 cut(s) 388, 403, 1132
BshTI ACCGGT 1 cut(s) 1131
BshVI ATCGAT 2 cut(s) 414, 936
BsiEI CGRYCG 3 cut(s) 388, 403, 1132
BsiSI CCGG 7 cut(s) 249, 278, 315, 384, 883, 899, 1132
BslFI GGGAC 5 cut(s) 231, 362, 546, 1238, 1243
BslI CCNNNNNNNGG 5 cut(s) 248, 552, 553, 1240, 1257
BsmAI GTCTC 4 cut(s) 111, 161, 356, 1169
BsmBI CGTCTC 2 cut(s) 111, 1169
BsmFI GGGAC 5 cut(s) 231, 362, 546, 1238, 1243
BsmI GAATGC 1 cut(s) 332
Bso31I GGTCTC 1 cut(s) 356
Bsp119I TTCGAA 1 cut(s) 302
Bsp19I CCATGG 1 cut(s) 487
BspACI CCGC 7 cut(s) 65, 425, 604, 744, 1104, 1146, 1329
BspCNI CTCAG 2 cut(s) 441, 1049
BspDI ATCGAT 2 cut(s) 414, 936
BspFNI CGCG 1 cut(s) 1097
BspLI GGNNCC 6 cut(s) 493, 1150, 1231, 1232, 1285, 1296
BspMAI CTGCAG 1 cut(s) 331
BspPI GGATC 6 cut(s) 481, 520, 606, 890, 1196, 1223
BspT104I TTCGAA 1 cut(s) 302
BspTNI GGTCTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 1131
BsrI ACTGG 1 cut(s) 1226
BssAI RCCGGY 1 cut(s) 1131
BssECI CCNNGG 3 cut(s) 315, 487, 547
BssT1I CCWWGG 2 cut(s) 487, 547
Bst4CI ACNGT 3 cut(s) 520, 1112, 1210
Bst6I CTCTTC 2 cut(s) 215, 660
BstBI TTCGAA 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 724
BstDEI CTNAG 2 cut(s) 449, 1036
BstDSI CCRYGG 1 cut(s) 487
BstF5I GGATG 1 cut(s) 1033
BstFNI CGCG 1 cut(s) 1097
BstMAI GTCTC 4 cut(s) 111, 161, 356, 1169
BstMCI CGRYCG 3 cut(s) 388, 403, 1132
BstMWI GCNNNNNNNGC 3 cut(s) 500, 633, 858
BstPAI GACNNNNGTC 1 cut(s) 402
BstSCI CCNGG 2 cut(s) 247, 314
BstSFI CTRYAG 1 cut(s) 327
BstUI CGCG 1 cut(s) 1097
BstV1I GCAGC 6 cut(s) 341, 592, 642, 848, 997, 1259
BstX2I RGATCY 1 cut(s) 997
BstYI RGATCY 1 cut(s) 997
BstZI CGGCCG 2 cut(s) 385, 400
Bsu15I ATCGAT 2 cut(s) 414, 936
BsuI GTATCC 1 cut(s) 831
BsuTUI ATCGAT 2 cut(s) 414, 936
BtgI CCRYGG 1 cut(s) 487
BtgZI GCGATG 2 cut(s) 1078, 1212
BtrI CACGTC 2 cut(s) 376, 577
BtsCI GGATG 1 cut(s) 1033
BtsI GCAGTG 2 cut(s) 233, 717
BtsIMutI CAGTG 3 cut(s) 233, 717, 891
Cac8I GCNNGC 1 cut(s) 724
Cfr10I RCCGGY 1 cut(s) 1131
Cfr13I GGNCC 8 cut(s) 263, 741, 851, 880, 901, 1149, 1230, 1294
ClaI ATCGAT 2 cut(s) 414, 936
Csp6I GTAC 5 cut(s) 8, 670, 776, 1045, 1135
CspAI ACCGGT 1 cut(s) 1131
CviAII CATG 6 cut(s) 44, 178, 203, 267, 488, 1194
CviQI GTAC 5 cut(s) 8, 670, 776, 1045, 1135
DdeI CTNAG 2 cut(s) 449, 1036
DraI TTTAAA 1 cut(s) 24
EaeI YGGCCR 5 cut(s) 279, 385, 400, 703, 945
EagI CGGCCG 2 cut(s) 385, 400
Eam1104I CTCTTC 2 cut(s) 215, 660
EarI CTCTTC 2 cut(s) 215, 660
EclXI CGGCCG 2 cut(s) 385, 400
Eco130I CCWWGG 2 cut(s) 487, 547
Eco31I GGTCTC 1 cut(s) 356
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 4 cut(s) 741, 880, 1230, 1294
Eco52I CGGCCG 2 cut(s) 385, 400
Eco57I CTGAAG 1 cut(s) 466
EcoO109I RGGNCCY 1 cut(s) 1230
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 2 cut(s) 487, 547
ErhI CCWWGG 2 cut(s) 487, 547
Esp3I CGTCTC 2 cut(s) 111, 1169
FaeI CATG 6 cut(s) 47, 181, 206, 270, 491, 1197
FalI AAGNNNNNCTT 2 cut(s) 81, 113
FaqI GGGAC 5 cut(s) 231, 362, 546, 1238, 1243
FatI CATG 6 cut(s) 43, 177, 202, 266, 487, 1193
FauI CCCGC 1 cut(s) 1139
FauNDI CATATG 1 cut(s) 772
FbaI TGATCA 2 cut(s) 814, 1161
FblI GTMKAC 3 cut(s) 129, 137, 1032
Fnu4HI GCNGC 6 cut(s) 330, 581, 656, 862, 1011, 1248
FokI GGATG 1 cut(s) 1040
Fsp4HI GCNGC 6 cut(s) 330, 581, 656, 862, 1011, 1248
GluI GCNGC 6 cut(s) 330, 581, 656, 862, 1011, 1248
GsaI CCCAGC 1 cut(s) 787
HapII CCGG 7 cut(s) 249, 278, 315, 384, 883, 899, 1132
Hin1II CATG 6 cut(s) 47, 181, 206, 270, 491, 1197
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 6 cut(s) 154, 244, 299, 464, 559, 1084
HpaII CCGG 7 cut(s) 249, 278, 315, 384, 883, 899, 1132
HphI GGTGA 3 cut(s) 772, 1009, 1048
Hpy166II GTNNAC 5 cut(s) 130, 138, 1033, 1047, 1279
Hpy188III TCNNGA 4 cut(s) 461, 646, 802, 991
Hpy8I GTNNAC 5 cut(s) 130, 138, 1033, 1047, 1279
Hpy99I CGWCG 2 cut(s) 106, 401
HpyAV CCTTC 1 cut(s) 655
HpyCH4III ACNGT 3 cut(s) 520, 1112, 1210
HpyCH4IV ACGT 3 cut(s) 375, 576, 1178
HpyCH4V TGCA 8 cut(s) 4, 168, 258, 329, 455, 580, 926, 1013
HpyF10VI GCNNNNNNNGC 3 cut(s) 500, 633, 858
HpyF3I CTNAG 2 cut(s) 449, 1036
HpySE526I ACGT 3 cut(s) 375, 576, 1178
Hsp92II CATG 6 cut(s) 47, 181, 206, 270, 491, 1197
KflI GGGWCCC 1 cut(s) 1230
Ksp22I TGATCA 2 cut(s) 814, 1161
LmnI GCTCC 2 cut(s) 491, 1289
Lsp1109I GCAGC 6 cut(s) 341, 592, 642, 848, 997, 1259
MaeII ACGT 3 cut(s) 375, 576, 1178
MaeIII GTNAC 2 cut(s) 889, 1106
MboII GAAGA 6 cut(s) 202, 578, 677, 758, 837, 992
MfeI CAATTG 1 cut(s) 736
MflI RGATCY 1 cut(s) 997
MlsI TGGCCA 1 cut(s) 947
MluNI TGGCCA 1 cut(s) 947
MlyI GAGTC 4 cut(s) 163, 253, 458, 568
MmeI TCCRAC 4 cut(s) 420, 621, 1229, 1291
MnlI CCTC 9 cut(s) 105, 453, 537, 815, 875, 934, 1061, 1100, 1237
Mox20I TGGCCA 1 cut(s) 947
MscI TGGCCA 1 cut(s) 947
MseI TTAA 3 cut(s) 23, 194, 968
MslI CAYNNNNRTG 2 cut(s) 450, 1022
Msp20I TGGCCA 1 cut(s) 947
MspA1I CMGCKG 1 cut(s) 509
MspI CCGG 7 cut(s) 249, 278, 315, 384, 883, 899, 1132
MspR9I CCNGG 2 cut(s) 249, 316
MunI CAATTG 1 cut(s) 736
Mva1269I GAATGC 1 cut(s) 332
MvnI CGCG 1 cut(s) 1097
MwoI GCNNNNNNNGC 3 cut(s) 500, 633, 858
NciI CCSGG 2 cut(s) 249, 316
NcoI CCATGG 1 cut(s) 487
NdeI CATATG 1 cut(s) 772
NlaIII CATG 6 cut(s) 47, 181, 206, 270, 491, 1197
NlaIV GGNNCC 6 cut(s) 493, 1150, 1231, 1232, 1285, 1296
NmuCI GTSAC 2 cut(s) 889, 1106
NspV TTCGAA 1 cut(s) 302
OliI CACNNNNGTG 1 cut(s) 1022
PctI GAATGC 1 cut(s) 332
PfeI GAWTC 2 cut(s) 299, 1084
PflMI CCANNNNNTGG 1 cut(s) 1257
PinAI ACCGGT 1 cut(s) 1131
PkrI GCNGC 6 cut(s) 331, 582, 657, 863, 1012, 1249
PleI GAGTC 4 cut(s) 162, 252, 458, 567
PpsI GAGTC 4 cut(s) 162, 252, 458, 567
PpuMI RGGWCCY 1 cut(s) 1230
PshAI GACNNNNGTC 1 cut(s) 402
Psp5II RGGWCCY 1 cut(s) 1230
PspFI CCCAGC 1 cut(s) 783
PspN4I GGNNCC 6 cut(s) 493, 1150, 1231, 1232, 1285, 1296
PspPI GGNCC 8 cut(s) 263, 741, 851, 880, 901, 1149, 1230, 1294
PspPPI RGGWCCY 1 cut(s) 1230
PstI CTGCAG 1 cut(s) 331
PsuI RGATCY 1 cut(s) 997
PvuII CAGCTG 1 cut(s) 509
RsaI GTAC 5 cut(s) 9, 671, 777, 1046, 1136
RsaNI GTAC 5 cut(s) 8, 670, 776, 1045, 1135
RseI CAYNNNNRTG 2 cut(s) 450, 1022
SaqAI TTAA 3 cut(s) 23, 194, 968
SatI GCNGC 6 cut(s) 330, 581, 656, 862, 1011, 1248
Sau96I GGNCC 8 cut(s) 263, 741, 851, 880, 901, 1149, 1230, 1294
ScaI AGTACT 2 cut(s) 9, 671
SchI GAGTC 4 cut(s) 163, 253, 458, 568
ScrFI CCNGG 2 cut(s) 249, 316
SfcI CTRYAG 1 cut(s) 327
SfuI TTCGAA 1 cut(s) 302
SinI GGWCC 4 cut(s) 741, 880, 1230, 1294
SmiMI CAYNNNNRTG 2 cut(s) 450, 1022
SmlI CTYRAG 3 cut(s) 151, 678, 989
SmoI CTYRAG 3 cut(s) 151, 678, 989
SsiI CCGC 7 cut(s) 65, 425, 604, 744, 1104, 1146, 1329
StyD4I CCNGG 2 cut(s) 247, 314
StyI CCWWGG 2 cut(s) 487, 547
TaaI ACNGT 3 cut(s) 520, 1112, 1210
TaiI ACGT 3 cut(s) 378, 579, 1181
TaqI TCGA 6 cut(s) 302, 414, 467, 645, 936, 1128
TatI WGTACW 4 cut(s) 7, 669, 775, 1044
TfiI GAWTC 2 cut(s) 299, 1084
Tru1I TTAA 3 cut(s) 23, 194, 968
Tru9I TTAA 3 cut(s) 23, 194, 968
TscAI CASTG 3 cut(s) 240, 724, 898
TseFI GTSAC 2 cut(s) 889, 1106
TseI GCWGC 6 cut(s) 329, 580, 655, 861, 1010, 1247
Tsp45I GTSAC 2 cut(s) 889, 1106
TspDTI ATGAA 1 cut(s) 409
TspGWI ACGGA 4 cut(s) 372, 993, 1187, 1256
TspRI CASTG 3 cut(s) 240, 724, 898
Van91I CCANNNNNTGG 1 cut(s) 1257
VpaK11BI GGWCC 4 cut(s) 741, 880, 1230, 1294
XapI RAATTY 3 cut(s) 182, 618, 950
XmiI GTMKAC 3 cut(s) 129, 137, 1032
ZrmI AGTACT 2 cut(s) 9, 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.