pycom16g08510

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014950
Physical Location & Seq
Forward (+)
5639471 .. 5643694
4224 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08510.1

Sequence Viewer

Length: 1206 bp
ATGCCTAAAACCATTCTCTCTCTCTCTCTCTCTCTATACACTCAACACATGGAATTTGTTTTCTTAAAACATCATGTTCTCTTCGGCCTTGTAATTTGGGCAACACTACTTCCGAGTCCCGGCATTTGCACAGGGCCATGCTCTTTTCCGGCCATATTCAATTTCGGAGATTCGAACTCGGACACCGGGGGTTTATCTGCAGCATTCGGACAAGCCCCTTACCCCAACGGAGAGACCTACTTTCACGTCCCGTCCGGCCGTTTCTCCGACGGCCGTCTTGTAATCGATTTCATAGCGGAAGGCTTGGGGTTGCCTCATCTGAGTGCATTTCTTGACTCGATGGGATCAAACTTCGGCCATGGAGCCAATTTTGCCACAGCTGGATCAACCGTTATACCTCCAAACACAACCATTTCCCAAAGTGGGGCGAGTCCCATCTCATTGGACGTGCAGCTTCTTCAATTCTCCGACTTCCGCACACGATCCCAAATTTATCGCACAAAAGGTGAATTATTTGAGAAATCGCTGCCGAAGGAAGAGTACTTTTCTCAAGCCCTATATACCTTTGACATCGGCCAAAATGATATCACTGCTGGCTACAAACTCAATTGGACCGCAGAACAAGTCAAGGCATATCTTCCATATGTGCTCACCAAGCTCTCAAATGCCATCAAGAGTGTATATGGTCAAGGAGGAAGATCGTTTTGGATACATAACACAGGCCCGCTGGGCTGCCTCCCTTATGTTTTGGTCCGCTTCGTCACTGATCCGGCCCAAATAAATAAATATGGGTGTGCGGATCAGATCAATGAGGTGGCCCAATTTTTCAATCAAAGATTAAAGGAAGCTGTGCTTCAACTCAAGAAAGATCTTCCATTGGCTGCAATCAACTATGTGGATGTCTACTCAGTAAAGTACACCCTCATCACCCAAGCCAAAATATATGGTTTTGAGAATCCCCTCATCGCATGTTGTCGTCACGGTGGCAAATATAACTTCGACCGGTACGCAAAGTGTGGGGCCAAGAAAATTATCAACGGCACAGAGACGTTGATAGCCAAATCGTGCGATGATCCAACGGTTCGGATCAATTGGGACGGGACCCACTTCACGGAGGCAGCCAACAAGTGGATATTCCAACAAATTGTGAATGGCTCCCTTTCGGACCCACCAAACCCGTTGAAAACGGCCTGTCGTTGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016298 GO:0016787 GO:0016788
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

402

Amino Acids

44.23

Weight (kDa)

8.23

Isoelectric Point (pI)

25.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 52 - 382 1.2e-46 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014950)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G26430
fragaria_vesca FvH4_4g26020
malus_domestica MD16G1100400.v1.1
prunus_persica Prupe.1G246600_v2.0.a1 Prupe.1G246600_v2.0.a1
pyrus_communis pycom16g08510
rosa_chinensis RchiOBHm_Chr4g0433461
rosa_laevigata RLG00000006751
rosa_multiflora Rmu_sc0000312.1_g000001
rosa_roxburghii Rroxscaffold_5G00375000
rosa_rugosa Rorug04G0269000
rosa_samantha Rh4BG331600 Rh4CG346900 Rh4DG327900
rosa_wichuraiana Rw0G020090 Rw4G028040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1128
AccI GTMKAC 1 cut(s) 903
AciI CCGC 6 cut(s) 296, 475, 615, 725, 754, 797
AclWI GGATC 7 cut(s) 352, 391, 477, 761, 807, 1067, 1094
AcoI YGGCCR 5 cut(s) 150, 256, 271, 355, 574
AcsI RAATTY 2 cut(s) 53, 489
AfaI GTAC 3 cut(s) 542, 917, 1007
AfiI CCNNNNNNNGG 5 cut(s) 119, 423, 424, 1111, 1128
AgeI ACCGGT 1 cut(s) 1002
AgsI TTSAA 5 cut(s) 160, 461, 829, 857, 1183
AjiI CACGTC 2 cut(s) 247, 448
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AluBI AGCT 4 cut(s) 380, 454, 658, 848
AluI AGCT 4 cut(s) 380, 454, 658, 848
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 2 cut(s) 227, 1040
AlwI GGATC 7 cut(s) 352, 391, 477, 761, 807, 1067, 1094
ApeKI GCWGC 6 cut(s) 200, 451, 526, 732, 881, 1118
ApoI RAATTY 2 cut(s) 53, 489
AsiGI ACCGGT 1 cut(s) 1002
AspS9I GGNCC 9 cut(s) 134, 612, 722, 751, 772, 817, 1020, 1101, 1165
AsuC2I CCSGG 2 cut(s) 120, 187
AsuHPI GGTGA 3 cut(s) 518, 643, 919
AsuII TTCGAA 1 cut(s) 173
AvaII GGWCC 4 cut(s) 612, 751, 1101, 1165
BarI GAAGNNNNNNTAC 2 cut(s) 524, 556
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 6 cut(s) 212, 463, 513, 719, 868, 1130
BccI CCATC 3 cut(s) 334, 443, 677
BceAI ACGGC 5 cut(s) 243, 258, 286, 1054, 1203
BciVI GTATCC 1 cut(s) 702
BcnI CCSGG 2 cut(s) 120, 187
BcoDI GTCTC 2 cut(s) 227, 1040
BfmI CTRYAG 1 cut(s) 198
BfuI GTATCC 1 cut(s) 702
BglI GCCNNNNNGGC 1 cut(s) 729
BglII AGATCT 1 cut(s) 868
BisI GCNGC 6 cut(s) 201, 452, 527, 733, 882, 1119
BlsI GCNGC 6 cut(s) 202, 453, 528, 734, 883, 1120
BmcAI AGTACT 1 cut(s) 542
Bme1390I CCNGG 2 cut(s) 120, 187
Bme18I GGWCC 4 cut(s) 612, 751, 1101, 1165
BmgBI CACGTC 2 cut(s) 247, 448
BmgT120I GGNCC 9 cut(s) 134, 612, 722, 751, 772, 817, 1020, 1101, 1165
BmiI GGNNCC 6 cut(s) 364, 1021, 1102, 1103, 1156, 1167
BmrFI CCNGG 2 cut(s) 120, 187
BoxI GACNNNNGTC 1 cut(s) 273
Bpu14I TTCGAA 1 cut(s) 173
BpuEI CTTGAG 2 cut(s) 534, 845
BpuMI CCSGG 2 cut(s) 120, 187
Bsa29I ATCGAT 1 cut(s) 285
BsaI GGTCTC 1 cut(s) 227
BsaJI CCNNGG 2 cut(s) 186, 358
BsaWI WCCGGW 1 cut(s) 1002
Bsc4I CCNNNNNNNGG 5 cut(s) 119, 423, 424, 1111, 1128
Bse118I RCCGGY 1 cut(s) 1002
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 186, 358
BseGI GGATG 1 cut(s) 904
BseLI CCNNNNNNNGG 5 cut(s) 119, 423, 424, 1111, 1128
BseMII CTCAG 2 cut(s) 311, 921
BseX3I CGGCCG 2 cut(s) 256, 271
BseXI GCAGC 6 cut(s) 212, 463, 513, 719, 868, 1130
BseYI CCCAGC 1 cut(s) 727
BsgI GTGCAG 1 cut(s) 470
Bsh1285I CGRYCG 3 cut(s) 259, 274, 1003
BshTI ACCGGT 1 cut(s) 1002
BshVI ATCGAT 1 cut(s) 285
BsiEI CGRYCG 3 cut(s) 259, 274, 1003
BsiHKAI GWGCWC 1 cut(s) 651
BsiSI CCGG 6 cut(s) 120, 149, 186, 255, 770, 1003
BslFI GGGAC 5 cut(s) 102, 233, 417, 1109, 1114
BslI CCNNNNNNNGG 5 cut(s) 119, 423, 424, 1111, 1128
BsmAI GTCTC 2 cut(s) 227, 1040
BsmBI CGTCTC 1 cut(s) 1040
BsmFI GGGAC 5 cut(s) 102, 233, 417, 1109, 1114
BsmI GAATGC 1 cut(s) 203
Bso31I GGTCTC 1 cut(s) 227
Bsp119I TTCGAA 1 cut(s) 173
Bsp1286I GDGCHC 1 cut(s) 651
Bsp19I CCATGG 1 cut(s) 358
BspACI CCGC 6 cut(s) 296, 475, 615, 725, 754, 797
BspCNI CTCAG 2 cut(s) 312, 920
BspDI ATCGAT 1 cut(s) 285
BspLI GGNNCC 6 cut(s) 364, 1021, 1102, 1103, 1156, 1167
BspMAI CTGCAG 1 cut(s) 202
BspPI GGATC 7 cut(s) 352, 391, 477, 761, 807, 1067, 1094
BspT104I TTCGAA 1 cut(s) 173
BspTNI GGTCTC 1 cut(s) 227
BsrFI RCCGGY 1 cut(s) 1002
BssAI RCCGGY 1 cut(s) 1002
BssECI CCNNGG 2 cut(s) 186, 358
BssT1I CCWWGG 1 cut(s) 358
Bst4CI ACNGT 3 cut(s) 391, 983, 1081
Bst6I CTCTTC 2 cut(s) 86, 531
BstBI TTCGAA 1 cut(s) 173
BstC8I GCNNGC 2 cut(s) 595, 725
BstDEI CTNAG 2 cut(s) 320, 907
BstDSI CCRYGG 1 cut(s) 358
BstF5I GGATG 1 cut(s) 904
BstMAI GTCTC 2 cut(s) 227, 1040
BstMCI CGRYCG 3 cut(s) 259, 274, 1003
BstMWI GCNNNNNNNGC 3 cut(s) 371, 655, 729
BstNSI RCATGY 1 cut(s) 972
BstPAI GACNNNNGTC 1 cut(s) 273
BstSCI CCNGG 2 cut(s) 118, 185
BstSFI CTRYAG 1 cut(s) 198
BstV1I GCAGC 6 cut(s) 212, 463, 513, 719, 868, 1130
BstX2I RGATCY 1 cut(s) 868
BstXI CCANNNNNNTGG 1 cut(s) 442
BstYI RGATCY 1 cut(s) 868
BstZI CGGCCG 2 cut(s) 256, 271
Bsu15I ATCGAT 1 cut(s) 285
BsuI GTATCC 1 cut(s) 702
BsuTUI ATCGAT 1 cut(s) 285
BtgI CCRYGG 1 cut(s) 358
BtgZI GCGATG 2 cut(s) 949, 1083
BtrI CACGTC 2 cut(s) 247, 448
BtsCI GGATG 1 cut(s) 904
BtsI GCAGTG 1 cut(s) 588
BtsIMutI CAGTG 2 cut(s) 588, 762
Cac8I GCNNGC 2 cut(s) 595, 725
Cfr10I RCCGGY 1 cut(s) 1002
Cfr13I GGNCC 9 cut(s) 134, 612, 722, 751, 772, 817, 1020, 1101, 1165
ClaI ATCGAT 1 cut(s) 285
Csp6I GTAC 3 cut(s) 541, 916, 1006
CspAI ACCGGT 1 cut(s) 1002
CviAII CATG 5 cut(s) 49, 74, 138, 359, 969
CviQI GTAC 3 cut(s) 541, 916, 1006
DdeI CTNAG 2 cut(s) 320, 907
EaeI YGGCCR 5 cut(s) 150, 256, 271, 355, 574
EagI CGGCCG 2 cut(s) 256, 271
Eam1104I CTCTTC 2 cut(s) 86, 531
EarI CTCTTC 2 cut(s) 86, 531
EclXI CGGCCG 2 cut(s) 256, 271
Eco130I CCWWGG 1 cut(s) 358
Eco31I GGTCTC 1 cut(s) 227
Eco32I GATATC 1 cut(s) 586
Eco47I GGWCC 4 cut(s) 612, 751, 1101, 1165
Eco52I CGGCCG 2 cut(s) 256, 271
EcoO109I RGGNCCY 1 cut(s) 1101
EcoRV GATATC 1 cut(s) 586
EcoT14I CCWWGG 1 cut(s) 358
ErhI CCWWGG 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 1040
FaeI CATG 5 cut(s) 52, 77, 141, 362, 972
FalI AAGNNNNNCTT 2 cut(s) 837, 869
FaqI GGGAC 5 cut(s) 102, 233, 417, 1109, 1114
FatI CATG 5 cut(s) 48, 73, 137, 358, 968
FauI CCCGC 1 cut(s) 732
FauNDI CATATG 1 cut(s) 643
FblI GTMKAC 1 cut(s) 903
Fnu4HI GCNGC 6 cut(s) 201, 452, 527, 733, 882, 1119
FokI GGATG 1 cut(s) 911
Fsp4HI GCNGC 6 cut(s) 201, 452, 527, 733, 882, 1119
GluI GCNGC 6 cut(s) 201, 452, 527, 733, 882, 1119
GsaI CCCAGC 1 cut(s) 731
HapII CCGG 6 cut(s) 120, 149, 186, 255, 770, 1003
Hin1II CATG 5 cut(s) 52, 77, 141, 362, 972
HinfI GANTC 5 cut(s) 115, 170, 335, 430, 955
HpaII CCGG 6 cut(s) 120, 149, 186, 255, 770, 1003
HphI GGTGA 3 cut(s) 518, 643, 919
Hpy166II GTNNAC 2 cut(s) 904, 918
Hpy188III TCNNGA 3 cut(s) 332, 673, 862
Hpy8I GTNNAC 2 cut(s) 904, 918
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 2 cut(s) 293, 526
HpyCH4III ACNGT 3 cut(s) 391, 983, 1081
HpyCH4IV ACGT 3 cut(s) 246, 447, 1049
HpyCH4V TGCA 6 cut(s) 129, 200, 326, 451, 884, 1200
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 655, 729
HpyF3I CTNAG 2 cut(s) 320, 907
HpySE526I ACGT 3 cut(s) 246, 447, 1049
Hsp92II CATG 5 cut(s) 52, 77, 141, 362, 972
KflI GGGWCCC 1 cut(s) 1101
LmnI GCTCC 2 cut(s) 362, 1160
Lsp1109I GCAGC 6 cut(s) 212, 463, 513, 719, 868, 1130
MaeII ACGT 3 cut(s) 246, 447, 1049
MaeIII GTNAC 2 cut(s) 760, 977
MboII GAAGA 6 cut(s) 73, 449, 548, 629, 708, 863
MfeI CAATTG 2 cut(s) 607, 1090
MflI RGATCY 1 cut(s) 868
MhlI GDGCHC 1 cut(s) 651
MlyI GAGTC 3 cut(s) 124, 329, 439
MmeI TCCRAC 4 cut(s) 291, 492, 1100, 1162
MnlI CCTC 8 cut(s) 324, 408, 686, 746, 805, 932, 971, 1108
MseI TTAA 2 cut(s) 65, 839
MslI CAYNNNNRTG 1 cut(s) 321
MspA1I CMGCKG 2 cut(s) 380, 727
MspI CCGG 6 cut(s) 120, 149, 186, 255, 770, 1003
MspR9I CCNGG 2 cut(s) 120, 187
MunI CAATTG 2 cut(s) 607, 1090
Mva1269I GAATGC 1 cut(s) 203
MwoI GCNNNNNNNGC 3 cut(s) 371, 655, 729
NciI CCSGG 2 cut(s) 120, 187
NcoI CCATGG 1 cut(s) 358
NdeI CATATG 1 cut(s) 643
NlaIII CATG 5 cut(s) 52, 77, 141, 362, 972
NlaIV GGNNCC 6 cut(s) 364, 1021, 1102, 1103, 1156, 1167
NmuCI GTSAC 2 cut(s) 760, 977
NspI RCATGY 1 cut(s) 972
NspV TTCGAA 1 cut(s) 173
PctI GAATGC 1 cut(s) 203
PfeI GAWTC 2 cut(s) 170, 955
PflMI CCANNNNNTGG 1 cut(s) 1128
PinAI ACCGGT 1 cut(s) 1002
PkrI GCNGC 6 cut(s) 202, 453, 528, 734, 883, 1120
PleI GAGTC 3 cut(s) 123, 329, 438
PpsI GAGTC 3 cut(s) 123, 329, 438
PpuMI RGGWCCY 1 cut(s) 1101
PshAI GACNNNNGTC 1 cut(s) 273
Psp5II RGGWCCY 1 cut(s) 1101
PspFI CCCAGC 1 cut(s) 727
PspN4I GGNNCC 6 cut(s) 364, 1021, 1102, 1103, 1156, 1167
PspPI GGNCC 9 cut(s) 134, 612, 722, 751, 772, 817, 1020, 1101, 1165
PspPPI RGGWCCY 1 cut(s) 1101
PstI CTGCAG 1 cut(s) 202
PsuI RGATCY 1 cut(s) 868
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 3 cut(s) 542, 917, 1007
RsaNI GTAC 3 cut(s) 541, 916, 1006
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 2 cut(s) 65, 839
SatI GCNGC 6 cut(s) 201, 452, 527, 733, 882, 1119
Sau96I GGNCC 9 cut(s) 134, 612, 722, 751, 772, 817, 1020, 1101, 1165
ScaI AGTACT 1 cut(s) 542
SchI GAGTC 3 cut(s) 124, 329, 439
ScrFI CCNGG 2 cut(s) 120, 187
SduI GDGCHC 1 cut(s) 651
SfcI CTRYAG 1 cut(s) 198
SfuI TTCGAA 1 cut(s) 173
SinI GGWCC 4 cut(s) 612, 751, 1101, 1165
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 549, 860
SmoI CTYRAG 2 cut(s) 549, 860
SsiI CCGC 6 cut(s) 296, 475, 615, 725, 754, 797
StyD4I CCNGG 2 cut(s) 118, 185
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 3 cut(s) 391, 983, 1081
TaiI ACGT 3 cut(s) 249, 450, 1052
TaqI TCGA 4 cut(s) 173, 285, 338, 999
TatI WGTACW 2 cut(s) 540, 915
TfiI GAWTC 2 cut(s) 170, 955
Tru1I TTAA 2 cut(s) 65, 839
Tru9I TTAA 2 cut(s) 65, 839
TscAI CASTG 2 cut(s) 595, 769
TseFI GTSAC 2 cut(s) 760, 977
TseI GCWGC 6 cut(s) 200, 451, 526, 732, 881, 1118
Tsp45I GTSAC 2 cut(s) 760, 977
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 2 cut(s) 243, 1127
TspRI CASTG 2 cut(s) 595, 769
Van91I CCANNNNNTGG 1 cut(s) 1128
VpaK11BI GGWCC 4 cut(s) 612, 751, 1101, 1165
XapI RAATTY 2 cut(s) 53, 489
XceI RCATGY 1 cut(s) 972
XmiI GTMKAC 1 cut(s) 903
ZrmI AGTACT 1 cut(s) 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.