FvH4_4g27970

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0013447
Physical Location & Seq
Forward (+)
28763691 .. 28766346
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g27970.t1

Sequence Viewer

Length: 1140 bp
ATGATGCTTCAGTTTCGAGACCCAGCAAATCTTAAGACCAAGAAGATTGTGTTTGAAGAGGCATATGTTGCTCGTGATTCTGCAACACTTGAACAGCTGAAAGAGTTGAGCTCTAAGCGCAGGATTCTCGTGGAGTCTACTAATAAGAGCAGTCCCATTACTAAGGCCATTGCAAGGGAAATGTCTGGAGGGTTAACTTCACAGTCTGAACAGGTTCTTCAAAAACTAGAACTGTATCTTCCTGTGTTGGAAAATTTGCTCTTTCACATTGATTCAGTCAGCAGCAACGGACAGATAGTTCAGTGGACTTCAGAACTTAAGATACAATGGACTAGTGCTCTTAGTTCATCGTCCTTCTTTAAGTTTGGAGGTTCTAAATATTTTCAAATGGATAACTTGCGATTTGAGATTGTTATGACTCTTTTCCTCTATGGCGCAATCCTTCGGGAGAGAGCATCAGAAGTTCTTCCATCAGATGTTTTGCAATCCGTGACCCTTTTCAGAGAAGCTGCTGGTGTTTATTACTGTCTTGCTCATGAGGTCCTTCCTTCTCTACAGCCTTCAGTCCCTGTGGAAAAGCCACCAGAAGTTGTCCCATCTGTGTCCACTGTCATAAGCCTTATTTGTTTGGCTGAGGCCCAGGCAGTAACAATAAAGAGGGCTGAAGAAAAAGGTACCAGTTTTGGTCTTTTGGCAAAGCTTCACTATGGTATTGTAGAGTTGCTTGATGAAGCCACAGATTCTTTACATACAGCTTCCATGGAATGCAAAGATATCTCTTCGTTCTTAGTGGAATTCATCACATCTTGCAAAGTGTTACATGAGTTAAAAAGTCAGAAATACTTGGCTGAAAGCCTGAAGAATGATGGCCAATTCGGGGTTGCTGTTGGACTCCTTCGACGTGCACTGATCAATGCGAAAAGGAAAATACCAAGTGAACAATCTTGGAAATCTGTTATCAAGAAGGAAATCGATAATTTTGCCGAATTACTCAGGAAGCATGAACATGAAAATGAATTTGTGTGGCATGAGAAAGTCCCCTCTGGTGATGAGTTGCCAAAACCAGATGGTAACAAAATCGCCAGTATTATACCTTACATTCCCAAAAGATGGGAAAGAGAACTTGCTTTCAAGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

380

Amino Acids

42.73

Weight (kDa)

6.74

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 67 - 366 9.5e-27 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13310
fragaria_vesca FvH4_4g27970
malus_domestica MD13G1045500.v1.1
prunus_persica Prupe.1G306500_v2.0.a1
pyrus_communis pycom13g04000
rosa_chinensis RchiOBHm_Chr4g0435681
rosa_laevigata RLG00000006567
rosa_multiflora Rmu_sc0000051.1_g000013
rosa_roxburghii Rroxscaffold_5G00376890
rosa_rugosa Rorug04G0288200 Rorug04G0288300 Rorug04G0288400 Rorug04G0288500
rosa_samantha Rh4AG343100 Rh4BG351500 Rh4CG366100 Rh4DG345700
rosa_wichuraiana Rw4G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 674
AccB1I GGYRCC 1 cut(s) 674
AccB7I CCANNNNNTGG 1 cut(s) 1110
AccI GTMKAC 1 cut(s) 137
AcoI YGGCCR 1 cut(s) 868
AcsI RAATTY 3 cut(s) 253, 794, 1016
AcuI CTGAAG 4 cut(s) 294, 546, 684, 878
AfaI GTAC 1 cut(s) 676
AfiI CCNNNNNNNGG 3 cut(s) 174, 877, 1110
AflII CTTAAG 2 cut(s) 32, 317
AgsI TTSAA 5 cut(s) 56, 92, 221, 386, 1132
AhlI ACTAGT 1 cut(s) 332
AjiI CACGTC 1 cut(s) 902
AjnI CCWGG 1 cut(s) 639
AloI GAACNNNNNNTCC 2 cut(s) 282, 314
AluBI AGCT 5 cut(s) 97, 111, 509, 700, 755
AluI AGCT 5 cut(s) 97, 111, 509, 700, 755
Alw21I GWGCWC 3 cut(s) 113, 340, 907
Alw26I GTCTC 1 cut(s) 12
Alw44I GTGCAC 1 cut(s) 903
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 3 cut(s) 165, 636, 868
ApaLI GTGCAC 1 cut(s) 903
ApeKI GCWGC 2 cut(s) 282, 509
ApoI RAATTY 3 cut(s) 253, 794, 1016
Asp700I GAANNNNTTC 2 cut(s) 213, 465
Asp718I GGTACC 1 cut(s) 674
AspLEI GCGC 2 cut(s) 120, 437
AspS9I GGNCC 2 cut(s) 541, 637
AsuHPI GGTGA 1 cut(s) 1058
AvaII GGWCC 1 cut(s) 541
BaeGI GKGCMC 1 cut(s) 907
BalI TGGCCA 1 cut(s) 870
BanI GGYRCC 1 cut(s) 674
BanII GRGCYC 1 cut(s) 113
BauI CACGAG 2 cut(s) 72, 128
Bbv12I GWGCWC 3 cut(s) 113, 340, 907
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 2 cut(s) 294, 496
BccI CCATC 5 cut(s) 478, 604, 860, 1061, 1104
BcgI CGANNNNNNTGC 4 cut(s) 109, 143, 962, 996
BciT130I CCWGG 1 cut(s) 641
BclI TGATCA 1 cut(s) 909
BcoDI GTCTC 1 cut(s) 12
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 2 cut(s) 227, 333
BfmI CTRYAG 1 cut(s) 554
BfrI CTTAAG 2 cut(s) 32, 317
BisI GCNGC 2 cut(s) 283, 510
BlsI GCNGC 2 cut(s) 284, 511
Bme1390I CCNGG 1 cut(s) 641
Bme18I GGWCC 1 cut(s) 541
BmgBI CACGTC 1 cut(s) 902
BmgT120I GGNCC 2 cut(s) 541, 637
BmiI GGNNCC 1 cut(s) 676
BmrFI CCNGG 1 cut(s) 641
BmsI GCATC 1 cut(s) 464
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 633
Bsa29I ATCGAT 1 cut(s) 972
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 639, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 877, 1110
Bse1I ACTGG 2 cut(s) 678, 1083
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 641
BseCI ATCGAT 1 cut(s) 972
BseDI CCNNGG 2 cut(s) 639, 759
BseLI CCNNNNNNNGG 3 cut(s) 174, 877, 1110
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 624, 1006
BseNI ACTGG 2 cut(s) 678, 1083
BseSI GKGCMC 1 cut(s) 907
BseXI GCAGC 2 cut(s) 294, 496
BseYI CCCAGC 1 cut(s) 22
BshFI GGCC 3 cut(s) 167, 638, 870
BshNI GGYRCC 1 cut(s) 674
BshVI ATCGAT 1 cut(s) 972
BsiHKAI GWGCWC 3 cut(s) 113, 340, 907
BslFI GGGAC 4 cut(s) 138, 551, 578, 1022
BslI CCNNNNNNNGG 3 cut(s) 174, 877, 1110
BsmAI GTCTC 1 cut(s) 12
BsmFI GGGAC 4 cut(s) 138, 551, 578, 1022
BsmI GAATGC 1 cut(s) 770
BsnI GGCC 3 cut(s) 167, 638, 870
Bso31I GGTCTC 1 cut(s) 12
Bsp1286I GDGCHC 3 cut(s) 113, 340, 907
Bsp143I GATC 1 cut(s) 909
Bsp19I CCATGG 1 cut(s) 759
BspANI GGCC 3 cut(s) 167, 638, 870
BspCNI CTCAG 2 cut(s) 625, 1005
BspDI ATCGAT 1 cut(s) 972
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 676
BspT107I GGYRCC 1 cut(s) 674
BspTI CTTAAG 2 cut(s) 32, 317
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 2 cut(s) 678, 1083
BssECI CCNNGG 2 cut(s) 639, 759
BssMI GATC 1 cut(s) 909
BssSI CACGAG 2 cut(s) 72, 128
BssT1I CCWWGG 1 cut(s) 759
Bst2BI CACGAG 2 cut(s) 72, 128
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 4 cut(s) 204, 234, 527, 610
Bst6I CTCTTC 2 cut(s) 51, 784
BstAFI CTTAAG 2 cut(s) 32, 317
BstAPI GCANNNNNTGC 1 cut(s) 68
BstDEI CTNAG 6 cut(s) 114, 162, 341, 633, 787, 992
BstDSI CCRYGG 1 cut(s) 759
BstHHI GCGC 2 cut(s) 120, 437
BstKTI GATC 1 cut(s) 912
BstMAI GTCTC 1 cut(s) 12
BstMBI GATC 1 cut(s) 909
BstMWI GCNNNNNNNGC 2 cut(s) 68, 117
BstNI CCWGG 1 cut(s) 641
BstSCI CCNGG 1 cut(s) 639
BstSFI CTRYAG 1 cut(s) 554
BstSLI GKGCMC 1 cut(s) 907
BstV1I GCAGC 2 cut(s) 294, 496
Bsu15I ATCGAT 1 cut(s) 972
BsuRI GGCC 3 cut(s) 167, 638, 870
BsuTUI ATCGAT 1 cut(s) 972
BtgI CCRYGG 1 cut(s) 759
BtrI CACGTC 1 cut(s) 902
BtsIMutI CAGTG 3 cut(s) 308, 606, 905
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 535
CfoI GCGC 2 cut(s) 120, 437
Cfr13I GGNCC 2 cut(s) 541, 637
ClaI ATCGAT 1 cut(s) 972
Csp6I GTAC 1 cut(s) 675
CviAII CATG 6 cut(s) 536, 760, 821, 1001, 1007, 1028
CviQI GTAC 1 cut(s) 675
DdeI CTNAG 6 cut(s) 114, 162, 341, 633, 787, 992
DpnI GATC 1 cut(s) 911
DpnII GATC 1 cut(s) 909
EaeI YGGCCR 1 cut(s) 868
Eam1104I CTCTTC 2 cut(s) 51, 784
EarI CTCTTC 2 cut(s) 51, 784
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 12
Eco32I GATATC 1 cut(s) 775
Eco47I GGWCC 1 cut(s) 541
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 4 cut(s) 294, 546, 684, 878
EcoICRI GAGCTC 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 541
EcoRI GAATTC 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 639
EcoRV GATATC 1 cut(s) 775
EcoT14I CCWWGG 1 cut(s) 759
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 759
FaeI CATG 6 cut(s) 539, 763, 824, 1004, 1010, 1031
FaqI GGGAC 4 cut(s) 138, 551, 578, 1022
FatI CATG 6 cut(s) 535, 759, 820, 1000, 1006, 1027
FauNDI CATATG 1 cut(s) 64
FbaI TGATCA 1 cut(s) 909
FblI GTMKAC 1 cut(s) 137
Fnu4HI GCNGC 2 cut(s) 283, 510
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 2 cut(s) 283, 510
FspBI CTAG 2 cut(s) 227, 333
GlaI GCGC 2 cut(s) 119, 436
GluI GCNGC 2 cut(s) 283, 510
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 3 cut(s) 167, 638, 870
HhaI GCGC 2 cut(s) 120, 437
Hin1II CATG 6 cut(s) 539, 763, 824, 1004, 1010, 1031
Hin6I GCGC 2 cut(s) 118, 435
HinP1I GCGC 2 cut(s) 118, 435
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HindIII AAGCTT 1 cut(s) 698
HinfI GANTC 7 cut(s) 77, 124, 134, 272, 418, 740, 891
HpaI GTTAAC 1 cut(s) 195
HphI GGTGA 1 cut(s) 1058
Hpy166II GTNNAC 6 cut(s) 138, 195, 306, 606, 905, 938
Hpy188I TCNGA 6 cut(s) 208, 313, 460, 475, 503, 837
Hpy188III TCNNGA 8 cut(s) 17, 74, 186, 446, 536, 961, 994, 1132
Hpy8I GTNNAC 6 cut(s) 138, 195, 306, 606, 905, 938
Hpy99I CGWCG 1 cut(s) 903
HpyAV CCTTC 7 cut(s) 364, 452, 554, 558, 570, 905, 958
HpyCH4III ACNGT 4 cut(s) 204, 234, 527, 610
HpyCH4IV ACGT 1 cut(s) 901
HpyCH4V TGCA 6 cut(s) 83, 173, 484, 768, 810, 905
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 117
HpyF3I CTNAG 6 cut(s) 114, 162, 341, 633, 787, 992
HpySE526I ACGT 1 cut(s) 901
Hsp92II CATG 6 cut(s) 539, 763, 824, 1004, 1010, 1031
HspAI GCGC 2 cut(s) 118, 435
KpnI GGTACC 1 cut(s) 678
Ksp22I TGATCA 1 cut(s) 909
KspAI GTTAAC 1 cut(s) 195
Kzo9I GATC 1 cut(s) 909
Lsp1109I GCAGC 2 cut(s) 294, 496
LweI GCATC 1 cut(s) 464
MaeI CTAG 2 cut(s) 227, 333
MaeII ACGT 1 cut(s) 901
MaeIII GTNAC 4 cut(s) 490, 646, 816, 1070
MalI GATC 1 cut(s) 911
MboI GATC 1 cut(s) 909
MboII GAAGA 8 cut(s) 55, 68, 209, 230, 458, 677, 771, 871
MhlI GDGCHC 3 cut(s) 113, 340, 907
MlsI TGGCCA 1 cut(s) 870
MluCI AATT 6 cut(s) 253, 794, 872, 976, 986, 1016
MluNI TGGCCA 1 cut(s) 870
MlyI GAGTC 3 cut(s) 143, 412, 885
MmeI TCCRAC 2 cut(s) 228, 868
MnlI CCTC 8 cut(s) 52, 182, 362, 437, 532, 628, 651, 1051
Mox20I TGGCCA 1 cut(s) 870
MroXI GAANNNNTTC 2 cut(s) 213, 465
MscI TGGCCA 1 cut(s) 870
MseI TTAA 5 cut(s) 33, 194, 318, 360, 827
MslI CAYNNNNRTG 2 cut(s) 1005, 1011
Msp20I TGGCCA 1 cut(s) 870
MspA1I CMGCKG 1 cut(s) 97
MspCI CTTAAG 2 cut(s) 32, 317
MspR9I CCNGG 1 cut(s) 641
Mva1269I GAATGC 1 cut(s) 770
MvaI CCWGG 1 cut(s) 641
MwoI GCNNNNNNNGC 2 cut(s) 68, 117
NcoI CCATGG 1 cut(s) 759
NdeI CATATG 1 cut(s) 64
NdeII GATC 1 cut(s) 909
NlaIII CATG 6 cut(s) 539, 763, 824, 1004, 1010, 1031
NlaIV GGNNCC 1 cut(s) 676
NmuCI GTSAC 1 cut(s) 490
PagI TCATGA 1 cut(s) 535
PctI GAATGC 1 cut(s) 770
PdmI GAANNNNTTC 2 cut(s) 213, 465
PfeI GAWTC 4 cut(s) 77, 124, 272, 740
PflMI CCANNNNNTGG 1 cut(s) 1110
PkrI GCNGC 2 cut(s) 284, 511
PleI GAGTC 3 cut(s) 142, 412, 885
PpsI GAGTC 3 cut(s) 142, 412, 885
PpuMI RGGWCCY 1 cut(s) 541
Psp124BI GAGCTC 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 639
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 1 cut(s) 639
PspN4I GGNNCC 1 cut(s) 676
PspPI GGNCC 2 cut(s) 541, 637
PspPPI RGGWCCY 1 cut(s) 541
PsrI GAACNNNNNNTAC 2 cut(s) 306, 338
PstNI CAGNNNCTG 1 cut(s) 569
PvuII CAGCTG 1 cut(s) 97
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
RseI CAYNNNNRTG 2 cut(s) 1005, 1011
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 5 cut(s) 33, 194, 318, 360, 827
SatI GCNGC 2 cut(s) 283, 510
Sau3AI GATC 1 cut(s) 909
Sau96I GGNCC 2 cut(s) 541, 637
SchI GAGTC 3 cut(s) 143, 412, 885
ScrFI CCNGG 1 cut(s) 641
SduI GDGCHC 3 cut(s) 113, 340, 907
SfaNI GCATC 1 cut(s) 464
SfcI CTRYAG 1 cut(s) 554
SinI GGWCC 1 cut(s) 541
SmiMI CAYNNNNRTG 2 cut(s) 1005, 1011
SmlI CTYRAG 2 cut(s) 32, 317
SmoI CTYRAG 2 cut(s) 32, 317
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 6 cut(s) 253, 794, 872, 976, 986, 1016
SspI AATATT 1 cut(s) 380
SspMI CTAG 2 cut(s) 227, 333
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 639
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 4 cut(s) 204, 234, 527, 610
TaiI ACGT 1 cut(s) 904
TaqI TCGA 3 cut(s) 16, 898, 972
TasI AATT 6 cut(s) 253, 794, 872, 976, 986, 1016
TfiI GAWTC 4 cut(s) 77, 124, 272, 740
Tru1I TTAA 5 cut(s) 33, 194, 318, 360, 827
Tru9I TTAA 5 cut(s) 33, 194, 318, 360, 827
TscAI CASTG 3 cut(s) 308, 613, 912
TseFI GTSAC 1 cut(s) 490
TseI GCWGC 2 cut(s) 282, 509
Tsp45I GTSAC 1 cut(s) 490
TspDTI ATGAA 6 cut(s) 336, 744, 787, 1017, 1023, 1029
TspGWI ACGGA 2 cut(s) 303, 478
TspRI CASTG 3 cut(s) 308, 613, 912
Van91I CCANNNNNTGG 1 cut(s) 1110
Vha464I CTTAAG 2 cut(s) 32, 317
VneI GTGCAC 1 cut(s) 903
VpaK11BI GGWCC 1 cut(s) 541
XapI RAATTY 3 cut(s) 253, 794, 1016
XmiI GTMKAC 1 cut(s) 137
XmnI GAANNNNTTC 2 cut(s) 213, 465
XspI CTAG 2 cut(s) 227, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.