Rw4G029950

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0013447
Physical Location & Seq
Forward (+)
56594759 .. 56597496
2738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G029950.1

Sequence Viewer

Length: 864 bp
ATGATGCTGCATTTTCGAGACCCAGCAAACCTGAAGACCAAGAAGATTGTGTTTGAGGAGGCGTATGCTGCTTGTGATTCTGCAACACTTGAACAGCTGAAAGAGTTGAGCTCTAAGCGTAGGATTCTCGAGGAGTCAACTAATGAGAGCAGCCCCATTACTAAGGCCATTGCAAGGGAAATGTCTGGAGGGTTAACTTCCCAGTCTCAACAGGTTCTTCAAAAACTAGAACTGTATCTTCCAGTGTTGGAAAATTTATTCTTCCACATTGATTTGATCAGCAGCAACGAACAGATAGTTCAGTGGACTTCAGAACTCAAGATACAATGGACTAGTGCTCTTAGTTCATCATCGTTCTTTAACTTTGGAGGTTCTAAATATTTTCAAATAGATAACTTGCAATTTGAGATTGTTATGACTCTTTTCCTTTATGGCGCAATCCTTCGGGAGAGAGCATCAGAAGTTCTTCCATCAGACCTTTTGAAATCTGCGACCCTTTTCAGAGAAGCTGCTGGTGTTTATCACTATCTTGCTCATCAGGTCCTTCCTTCTCTACAGCCTTCAGTCCATGTGGAAAAGCCACCAGAAGTTGTCCCATCTGTGTCCACTGTCATGAGCCTTATTTGTTTGGCTGAGGCCCAGGCAGTAACAATAAAGAGGGCTGAAGAGAAGGGTACTACTGTTGGTCTGTTGGCAAAGTTGCACCATGGTGTTGTAGAGTTGCTTGATGAAGCCGCATGTGCTTTACATACAGCTTCCATGGAATGCAAAGATATTTCTTCGCGCTTTGTGGTATGTGCACATTTTGCACACTGTATGTTCATTTCAATTTTGGGAGAAAGTTCAATAATGTTTCCACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

287

Amino Acids

31.93

Weight (kDa)

5.7

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 70 - 264 9.4e-16 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13310
fragaria_vesca FvH4_4g27970
malus_domestica MD13G1045500.v1.1
prunus_persica Prupe.1G306500_v2.0.a1
pyrus_communis pycom13g04000
rosa_chinensis RchiOBHm_Chr4g0435681
rosa_laevigata RLG00000006567
rosa_multiflora Rmu_sc0000051.1_g000013
rosa_roxburghii Rroxscaffold_5G00376890
rosa_rugosa Rorug04G0288200 Rorug04G0288300 Rorug04G0288400 Rorug04G0288500
rosa_samantha Rh4AG343100 Rh4BG351500 Rh4CG366100 Rh4DG345700
rosa_wichuraiana Rw4G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 784
AciI CCGC 1 cut(s) 735
AcsI RAATTY 1 cut(s) 253
AcuI CTGAAG 4 cut(s) 53, 294, 546, 684
AfaI GTAC 1 cut(s) 676
AfiI CCNNNNNNNGG 1 cut(s) 174
AgsI TTSAA 6 cut(s) 92, 221, 386, 484, 828, 846
AhlI ACTAGT 1 cut(s) 332
AjnI CCWGG 1 cut(s) 639
AleI CACNNNNGTG 1 cut(s) 708
AluBI AGCT 4 cut(s) 97, 111, 509, 755
AluI AGCT 4 cut(s) 97, 111, 509, 755
Alw21I GWGCWC 3 cut(s) 113, 340, 802
Alw26I GTCTC 2 cut(s) 12, 210
Alw44I GTGCAC 1 cut(s) 798
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 2 cut(s) 165, 636
ApaLI GTGCAC 1 cut(s) 798
ApeKI GCWGC 5 cut(s) 7, 68, 150, 282, 509
ApoI RAATTY 1 cut(s) 253
Asp700I GAANNNNTTC 1 cut(s) 465
AspLEI GCGC 2 cut(s) 437, 786
AspS9I GGNCC 2 cut(s) 541, 637
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 541
BaeGI GKGCMC 1 cut(s) 802
BaeI ACNNNNGTAYC 2 cut(s) 666, 699
BanII GRGCYC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 3 cut(s) 113, 340, 802
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 4 cut(s) 55, 162, 294, 496
BccI CCATC 2 cut(s) 478, 604
BciT130I CCWGG 1 cut(s) 641
BclI TGATCA 1 cut(s) 276
BcoDI GTCTC 2 cut(s) 12, 210
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 2 cut(s) 227, 333
BfmI CTRYAG 1 cut(s) 554
BisI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
BlsI GCNGC 6 cut(s) 9, 70, 152, 284, 511, 736
Bme1390I CCNGG 1 cut(s) 641
Bme18I GGWCC 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 541, 637
BmrFI CCNGG 1 cut(s) 641
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 639, 706, 759
Bsc4I CCNNNNNNNGG 1 cut(s) 174
Bse1I ACTGG 2 cut(s) 202, 242
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 3 cut(s) 639, 706, 759
BseLI CCNNNNNNNGG 1 cut(s) 174
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 624
BseNI ACTGG 2 cut(s) 202, 242
BseRI GAGGAG 2 cut(s) 71, 146
BseSI GKGCMC 1 cut(s) 802
BseXI GCAGC 4 cut(s) 55, 162, 294, 496
BseYI CCCAGC 1 cut(s) 22
Bsh1236I CGCG 1 cut(s) 784
BshFI GGCC 2 cut(s) 167, 638
BsiHKAI GWGCWC 3 cut(s) 113, 340, 802
BsiHKCI CYCGRG 1 cut(s) 128
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 1 cut(s) 174
BsmAI GTCTC 2 cut(s) 12, 210
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 770
BsnI GGCC 2 cut(s) 167, 638
Bso31I GGTCTC 1 cut(s) 12
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 3 cut(s) 113, 340, 802
Bsp143I GATC 1 cut(s) 276
Bsp19I CCATGG 2 cut(s) 706, 759
BspACI CCGC 1 cut(s) 735
BspANI GGCC 2 cut(s) 167, 638
BspCNI CTCAG 1 cut(s) 625
BspFNI CGCG 1 cut(s) 784
BspHI TCATGA 1 cut(s) 612
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 2 cut(s) 202, 242
BssECI CCNNGG 3 cut(s) 639, 706, 759
BssMI GATC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 706, 759
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 4 cut(s) 234, 610, 682, 815
Bst6I CTCTTC 1 cut(s) 660
BstAPI GCANNNNNTGC 1 cut(s) 806
BstDEI CTNAG 4 cut(s) 114, 162, 341, 633
BstDSI CCRYGG 2 cut(s) 706, 759
BstFNI CGCG 1 cut(s) 784
BstHHI GCGC 2 cut(s) 437, 786
BstKTI GATC 1 cut(s) 279
BstMAI GTCTC 2 cut(s) 12, 210
BstMBI GATC 1 cut(s) 276
BstMWI GCNNNNNNNGC 3 cut(s) 68, 740, 806
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 639
BstSFI CTRYAG 1 cut(s) 554
BstSLI GKGCMC 1 cut(s) 802
BstUI CGCG 1 cut(s) 784
BstV1I GCAGC 4 cut(s) 55, 162, 294, 496
BstV2I GAAGAC 1 cut(s) 41
BsuRI GGCC 2 cut(s) 167, 638
BtgI CCRYGG 2 cut(s) 706, 759
BtsIMutI CAGTG 4 cut(s) 249, 308, 606, 811
CciI TCATGA 1 cut(s) 612
CfoI GCGC 2 cut(s) 437, 786
Cfr13I GGNCC 2 cut(s) 541, 637
Csp6I GTAC 1 cut(s) 675
CviAII CATG 5 cut(s) 569, 613, 707, 738, 760
CviQI GTAC 1 cut(s) 675
DdeI CTNAG 4 cut(s) 114, 162, 341, 633
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 706, 759
Eco24I GRGCYC 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 541
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 4 cut(s) 53, 294, 546, 684
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 111
EcoO109I RGGNCCY 1 cut(s) 541
EcoRII CCWGG 1 cut(s) 639
EcoT14I CCWWGG 2 cut(s) 706, 759
EcoT38I GRGCYC 1 cut(s) 113
ErhI CCWWGG 2 cut(s) 706, 759
FaeI CATG 5 cut(s) 572, 616, 710, 741, 763
FaqI GGGAC 1 cut(s) 578
FatI CATG 5 cut(s) 568, 612, 706, 737, 759
FbaI TGATCA 1 cut(s) 276
Fnu4HI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
FspBI CTAG 2 cut(s) 227, 333
GlaI GCGC 2 cut(s) 436, 785
GluI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 2 cut(s) 167, 638
HhaI GCGC 2 cut(s) 437, 786
Hin1II CATG 5 cut(s) 572, 616, 710, 741, 763
Hin6I GCGC 2 cut(s) 435, 784
HinP1I GCGC 2 cut(s) 435, 784
HincII GTYRAC 2 cut(s) 138, 195
HindII GTYRAC 2 cut(s) 138, 195
HinfI GANTC 4 cut(s) 77, 124, 134, 418
HpaI GTTAAC 1 cut(s) 195
Hpy166II GTNNAC 5 cut(s) 138, 195, 306, 606, 800
Hpy188I TCNGA 4 cut(s) 313, 460, 475, 503
Hpy188III TCNNGA 6 cut(s) 17, 128, 186, 319, 446, 613
Hpy8I GTNNAC 5 cut(s) 138, 195, 306, 606, 800
HpyAV CCTTC 5 cut(s) 452, 554, 558, 570, 664
HpyCH4III ACNGT 4 cut(s) 234, 610, 682, 815
HpyCH4V TGCA 8 cut(s) 10, 83, 173, 400, 703, 768, 800, 809
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 740, 806
HpyF3I CTNAG 4 cut(s) 114, 162, 341, 633
Hsp92II CATG 5 cut(s) 572, 616, 710, 741, 763
HspAI GCGC 2 cut(s) 435, 784
Ksp22I TGATCA 1 cut(s) 276
KspAI GTTAAC 1 cut(s) 195
Kzo9I GATC 1 cut(s) 276
Lsp1109I GCAGC 4 cut(s) 55, 162, 294, 496
LweI GCATC 1 cut(s) 464
MaeI CTAG 2 cut(s) 227, 333
MaeIII GTNAC 1 cut(s) 646
MalI GATC 1 cut(s) 278
MboI GATC 1 cut(s) 276
MboII GAAGA 8 cut(s) 46, 55, 209, 230, 253, 458, 677, 771
MhlI GDGCHC 3 cut(s) 113, 340, 802
MluCI AATT 3 cut(s) 253, 401, 828
MlyI GAGTC 2 cut(s) 143, 412
MmeI TCCRAC 1 cut(s) 228
MnlI CCTC 7 cut(s) 49, 52, 124, 182, 362, 628, 651
MroXI GAANNNNTTC 1 cut(s) 465
MseI TTAA 2 cut(s) 194, 360
MslI CAYNNNNRTG 2 cut(s) 611, 708
MspA1I CMGCKG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 641
Mva1269I GAATGC 1 cut(s) 770
MvaI CCWGG 1 cut(s) 641
MvnI CGCG 1 cut(s) 784
MwoI GCNNNNNNNGC 3 cut(s) 68, 740, 806
NcoI CCATGG 2 cut(s) 706, 759
NdeII GATC 1 cut(s) 276
NlaIII CATG 5 cut(s) 572, 616, 710, 741, 763
NspI RCATGY 1 cut(s) 741
OliI CACNNNNGTG 1 cut(s) 708
PaeR7I CTCGAG 1 cut(s) 128
PagI TCATGA 1 cut(s) 612
PctI GAATGC 1 cut(s) 770
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 2 cut(s) 77, 124
PkrI GCNGC 6 cut(s) 9, 70, 152, 284, 511, 736
PleI GAGTC 2 cut(s) 142, 412
PpsI GAGTC 2 cut(s) 142, 412
PpuMI RGGWCCY 1 cut(s) 541
Psp124BI GAGCTC 1 cut(s) 113
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 639
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 1 cut(s) 639
PspPI GGNCC 2 cut(s) 541, 637
PspPPI RGGWCCY 1 cut(s) 541
PsrI GAACNNNNNNTAC 2 cut(s) 306, 338
PvuII CAGCTG 1 cut(s) 97
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
RseI CAYNNNNRTG 2 cut(s) 611, 708
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 194, 360
SatI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
Sau3AI GATC 1 cut(s) 276
Sau96I GGNCC 2 cut(s) 541, 637
SchI GAGTC 2 cut(s) 143, 412
ScrFI CCNGG 1 cut(s) 641
SduI GDGCHC 3 cut(s) 113, 340, 802
SfaNI GCATC 1 cut(s) 464
SfcI CTRYAG 1 cut(s) 554
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 1 cut(s) 541
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 2 cut(s) 611, 708
SmlI CTYRAG 2 cut(s) 128, 317
SmoI CTYRAG 2 cut(s) 128, 317
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 3 cut(s) 253, 401, 828
SsiI CCGC 1 cut(s) 735
SspI AATATT 1 cut(s) 380
SspMI CTAG 2 cut(s) 227, 333
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 1 cut(s) 639
StyI CCWWGG 2 cut(s) 706, 759
TaaI ACNGT 4 cut(s) 234, 610, 682, 815
TaqI TCGA 2 cut(s) 16, 129
TasI AATT 3 cut(s) 253, 401, 828
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 2 cut(s) 77, 124
Tru1I TTAA 2 cut(s) 194, 360
Tru9I TTAA 2 cut(s) 194, 360
TscAI CASTG 4 cut(s) 249, 308, 613, 818
TseI GCWGC 5 cut(s) 7, 68, 150, 282, 509
TspDTI ATGAA 3 cut(s) 336, 744, 811
TspRI CASTG 4 cut(s) 249, 308, 613, 818
VneI GTGCAC 1 cut(s) 798
VpaK11BI GGWCC 1 cut(s) 541
XapI RAATTY 1 cut(s) 253
XceI RCATGY 1 cut(s) 741
XhoI CTCGAG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 2 cut(s) 227, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.