Rh4AG343100

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0013447
Physical Location & Seq
Forward (+)
65140282 .. 65142501
2220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG343100.1

Sequence Viewer

Length: 1146 bp
ATGATGCTGCATTTTCGAGACCCAGCAAACCTGAAGACCAAGAAGATTGTGTTTGAGGAGGCGTATGCTGCTTGTGATTCTGCAACACTTGAACAGCTGAAAGAGTTGAGCTCTATGCGTAGGATTCTCGAGGAGTCAACTAATGAGAGCAGCCCCATTACTAAGGCCATTGCAAGGGAAATGTCTGGAGGGTTAACTTCCCAGTCTCAACAGGTTCTTCAAAAACTAGAACTGTATCTTCCAGTGTTGGAAAATTTATTCTTCCACATTGATTTGATCAGCAGCAACGAACAGATAGTTCAGTGGACTTCAGAACTCAAGATACAATGGACTAGTGCTCTTAGTTCATCATCGTTCTTTAACTTTGGAGGTTCTAAATATTTTCAAATAGATAACTTGCAATTTGAGATTGTTATGACTCTTTTCCTTAATGGCGCAATCCTTCGGGAGAGAGCATCAGAAGTTCTTCCATCAGACCTATTGAAATCTGCGACCCTTTTCAGAGAAGCTGCTGGTGTTTATCACTATCTTGCTCATCAGGTCCTTCCTTCTCTACAGCCTTCAGTCCCTGTGGAAAAGCCACCAGAAGTTGTCCCATCTGTGTCCACTGTCATGAGCCTTATTTGTTTGGCTGAGGCCCAGGCAGTAACAATAAAGAGGGCTGAAGAGAAGGGTACTACTGTTGGTCTGTTGGCAAAGTTGCACCATGGTGTTGTAGAGTTGCTTGATGAAGCCGCATGTGCTTTACATACAGCTTCCATGGAATGCAAAGATATTTCTTCGCGCTTTGTGGAATTCATCACATCTTGCAAAGCGTTACATGAGTTAAAAAGTCAGAAATACTTGGCTGAAAGCCTGAAGAATAATAATGATGGCCAATTCGGGGTTTCTGTTGGACTTCTTCGACGTGCACTGATCAATGCGAAAAGGAAAATACCAAGTGAAGAATCTTGGAAATCTGTTTTCAAAAAGGAAATTGATGATTTTGCCGAATTACTCAGGAAGCATGAACATGAAAATGAATTTGTCTGGCATGAGAAAATCCCCTCTGGTGATGAGTTGCCAAAACCCGATGGTACCAAAATCGCGAGTGTTATACCTTACAATCCCAAAAGATGGGAAAGAGAGCTCGCCTTCAAGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

381

Amino Acids

42.92

Weight (kDa)

6.05

Isoelectric Point (pI)

49.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 70 - 368 6.4e-29 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13310
fragaria_vesca FvH4_4g27970
malus_domestica MD13G1045500.v1.1
prunus_persica Prupe.1G306500_v2.0.a1
pyrus_communis pycom13g04000
rosa_chinensis RchiOBHm_Chr4g0435681
rosa_laevigata RLG00000006567
rosa_multiflora Rmu_sc0000051.1_g000013
rosa_roxburghii Rroxscaffold_5G00376890
rosa_rugosa Rorug04G0288200 Rorug04G0288300 Rorug04G0288400 Rorug04G0288500
rosa_samantha Rh4AG343100 Rh4BG351500 Rh4CG366100 Rh4DG345700
rosa_wichuraiana Rw4G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1076
AccB1I GGYRCC 1 cut(s) 1076
AccB7I CCANNNNNTGG 1 cut(s) 1116
AccII CGCG 2 cut(s) 784, 1088
AciI CCGC 1 cut(s) 735
AcoI YGGCCR 1 cut(s) 874
AcsI RAATTY 3 cut(s) 253, 794, 1022
AcuI CTGAAG 5 cut(s) 53, 294, 546, 684, 878
AfaI GTAC 2 cut(s) 676, 1078
AfiI CCNNNNNNNGG 3 cut(s) 174, 883, 1116
AgsI TTSAA 6 cut(s) 92, 221, 386, 484, 967, 1138
AhlI ACTAGT 1 cut(s) 332
AjiI CACGTC 1 cut(s) 908
AjnI CCWGG 1 cut(s) 639
AleI CACNNNNGTG 1 cut(s) 708
AluBI AGCT 5 cut(s) 97, 111, 509, 755, 1129
AluI AGCT 5 cut(s) 97, 111, 509, 755, 1129
Alw21I GWGCWC 4 cut(s) 113, 340, 913, 1131
Alw26I GTCTC 2 cut(s) 12, 210
Alw44I GTGCAC 1 cut(s) 909
AlwNI CAGNNNCTG 1 cut(s) 569
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 3 cut(s) 165, 636, 874
ApaLI GTGCAC 1 cut(s) 909
ApeKI GCWGC 5 cut(s) 7, 68, 150, 282, 509
ApoI RAATTY 3 cut(s) 253, 794, 1022
Asp700I GAANNNNTTC 1 cut(s) 465
Asp718I GGTACC 1 cut(s) 1076
AspLEI GCGC 2 cut(s) 437, 786
AspS9I GGNCC 2 cut(s) 541, 637
AsuHPI GGTGA 1 cut(s) 1064
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 541
BaeGI GKGCMC 1 cut(s) 913
BaeI ACNNNNGTAYC 2 cut(s) 666, 699
BalI TGGCCA 1 cut(s) 876
BanI GGYRCC 1 cut(s) 1076
BanII GRGCYC 2 cut(s) 113, 1131
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 4 cut(s) 113, 340, 913, 1131
BbvCI CCTCAGC 1 cut(s) 633
BbvI GCAGC 4 cut(s) 55, 162, 294, 496
BccI CCATC 5 cut(s) 478, 604, 866, 1067, 1110
BciT130I CCWGG 1 cut(s) 641
BclI TGATCA 2 cut(s) 276, 915
BcoDI GTCTC 2 cut(s) 12, 210
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 2 cut(s) 227, 333
BfmI CTRYAG 1 cut(s) 554
BglII AGATCT 1 cut(s) 1140
BisI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
BlsI GCNGC 6 cut(s) 9, 70, 152, 284, 511, 736
Bme1390I CCNGG 1 cut(s) 641
Bme18I GGWCC 1 cut(s) 541
BmeT110I CYCGRG 1 cut(s) 128
BmgBI CACGTC 1 cut(s) 908
BmgT120I GGNCC 2 cut(s) 541, 637
BmiI GGNNCC 1 cut(s) 1078
BmrFI CCNGG 1 cut(s) 641
BmrI ACTGGG 1 cut(s) 196
BmsI GCATC 1 cut(s) 464
BmuI ACTGGG 1 cut(s) 196
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 95, 127
BpmI CTGGAG 1 cut(s) 207
Bpu10I CCTNAGC 1 cut(s) 633
BpuEI CTTGAG 1 cut(s) 302
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 639, 706, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 174, 883, 1116
Bse1I ACTGG 2 cut(s) 202, 242
Bse3DI GCAATG 1 cut(s) 168
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 3 cut(s) 639, 706, 759
BseLI CCNNNNNNNGG 3 cut(s) 174, 883, 1116
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 2 cut(s) 624, 1012
BseNI ACTGG 2 cut(s) 202, 242
BseRI GAGGAG 2 cut(s) 71, 146
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 4 cut(s) 55, 162, 294, 496
BseYI CCCAGC 1 cut(s) 22
Bsh1236I CGCG 2 cut(s) 784, 1088
BshFI GGCC 3 cut(s) 167, 638, 876
BshNI GGYRCC 1 cut(s) 1076
BsiHKAI GWGCWC 4 cut(s) 113, 340, 913, 1131
BsiHKCI CYCGRG 1 cut(s) 128
BslFI GGGAC 2 cut(s) 551, 578
BslI CCNNNNNNNGG 3 cut(s) 174, 883, 1116
BsmAI GTCTC 2 cut(s) 12, 210
BsmFI GGGAC 2 cut(s) 551, 578
BsmI GAATGC 1 cut(s) 770
BsnI GGCC 3 cut(s) 167, 638, 876
Bso31I GGTCTC 1 cut(s) 12
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 4 cut(s) 113, 340, 913, 1131
Bsp143I GATC 3 cut(s) 276, 915, 1140
Bsp19I CCATGG 2 cut(s) 706, 759
Bsp68I TCGCGA 1 cut(s) 1088
BspACI CCGC 1 cut(s) 735
BspANI GGCC 3 cut(s) 167, 638, 876
BspCNI CTCAG 2 cut(s) 625, 1011
BspFNI CGCG 2 cut(s) 784, 1088
BspHI TCATGA 1 cut(s) 612
BspLI GGNNCC 1 cut(s) 1078
BspT107I GGYRCC 1 cut(s) 1076
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 168
BsrI ACTGG 2 cut(s) 202, 242
BssECI CCNNGG 3 cut(s) 639, 706, 759
BssMI GATC 3 cut(s) 276, 915, 1140
BssT1I CCWWGG 2 cut(s) 706, 759
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 3 cut(s) 234, 610, 682
Bst6I CTCTTC 1 cut(s) 660
BstC8I GCNNGC 1 cut(s) 1131
BstDEI CTNAG 4 cut(s) 162, 341, 633, 998
BstDSI CCRYGG 2 cut(s) 706, 759
BstFNI CGCG 2 cut(s) 784, 1088
BstHHI GCGC 2 cut(s) 437, 786
BstKTI GATC 3 cut(s) 279, 918, 1143
BstMAI GTCTC 2 cut(s) 12, 210
BstMBI GATC 3 cut(s) 276, 915, 1140
BstMWI GCNNNNNNNGC 2 cut(s) 68, 740
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 1 cut(s) 741
BstSCI CCNGG 1 cut(s) 639
BstSFI CTRYAG 1 cut(s) 554
BstSLI GKGCMC 1 cut(s) 913
BstUI CGCG 2 cut(s) 784, 1088
BstV1I GCAGC 4 cut(s) 55, 162, 294, 496
BstV2I GAAGAC 1 cut(s) 41
BstX2I RGATCY 1 cut(s) 1140
BstYI RGATCY 1 cut(s) 1140
BsuRI GGCC 3 cut(s) 167, 638, 876
BtgI CCRYGG 2 cut(s) 706, 759
BtrI CACGTC 1 cut(s) 908
BtsIMutI CAGTG 4 cut(s) 249, 308, 606, 911
BtuMI TCGCGA 1 cut(s) 1088
Cac8I GCNNGC 1 cut(s) 1131
CaiI CAGNNNCTG 1 cut(s) 569
CciI TCATGA 1 cut(s) 612
CfoI GCGC 2 cut(s) 437, 786
Cfr13I GGNCC 2 cut(s) 541, 637
Csp6I GTAC 2 cut(s) 675, 1077
CviAII CATG 8 cut(s) 613, 707, 738, 760, 821, 1007, 1013, 1034
CviQI GTAC 2 cut(s) 675, 1077
DdeI CTNAG 4 cut(s) 162, 341, 633, 998
DpnI GATC 3 cut(s) 278, 917, 1142
DpnII GATC 3 cut(s) 276, 915, 1140
EaeI YGGCCR 1 cut(s) 874
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Ecl136II GAGCTC 2 cut(s) 111, 1129
Eco130I CCWWGG 2 cut(s) 706, 759
Eco24I GRGCYC 2 cut(s) 113, 1131
Eco31I GGTCTC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 541
Eco53kI GAGCTC 2 cut(s) 111, 1129
Eco57I CTGAAG 5 cut(s) 53, 294, 546, 684, 878
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 2 cut(s) 111, 1129
EcoO109I RGGNCCY 1 cut(s) 541
EcoRI GAATTC 1 cut(s) 794
EcoRII CCWGG 1 cut(s) 639
EcoT14I CCWWGG 2 cut(s) 706, 759
EcoT38I GRGCYC 2 cut(s) 113, 1131
ErhI CCWWGG 2 cut(s) 706, 759
FaeI CATG 8 cut(s) 616, 710, 741, 763, 824, 1010, 1016, 1037
FaqI GGGAC 2 cut(s) 551, 578
FatI CATG 8 cut(s) 612, 706, 737, 759, 820, 1006, 1012, 1033
FbaI TGATCA 2 cut(s) 276, 915
Fnu4HI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
FriOI GRGCYC 2 cut(s) 113, 1131
Fsp4HI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
FspBI CTAG 2 cut(s) 227, 333
GlaI GCGC 2 cut(s) 436, 785
GluI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
GsaI CCCAGC 1 cut(s) 26
GsuI CTGGAG 1 cut(s) 207
HaeIII GGCC 3 cut(s) 167, 638, 876
HhaI GCGC 2 cut(s) 437, 786
Hin1II CATG 8 cut(s) 616, 710, 741, 763, 824, 1010, 1016, 1037
Hin6I GCGC 2 cut(s) 435, 784
HinP1I GCGC 2 cut(s) 435, 784
HincII GTYRAC 2 cut(s) 138, 195
HindII GTYRAC 2 cut(s) 138, 195
HinfI GANTC 5 cut(s) 77, 124, 134, 418, 947
HpaI GTTAAC 1 cut(s) 195
HphI GGTGA 1 cut(s) 1064
Hpy166II GTNNAC 5 cut(s) 138, 195, 306, 606, 911
Hpy188I TCNGA 5 cut(s) 313, 460, 475, 503, 837
Hpy188III TCNNGA 9 cut(s) 17, 128, 186, 319, 446, 613, 1000, 1087, 1138
Hpy8I GTNNAC 5 cut(s) 138, 195, 306, 606, 911
Hpy99I CGWCG 1 cut(s) 909
HpyAV CCTTC 6 cut(s) 452, 554, 558, 570, 664, 1144
HpyCH4III ACNGT 3 cut(s) 234, 610, 682
HpyCH4IV ACGT 1 cut(s) 907
HpyCH4V TGCA 8 cut(s) 10, 83, 173, 400, 703, 768, 810, 911
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 740
HpyF3I CTNAG 4 cut(s) 162, 341, 633, 998
HpySE526I ACGT 1 cut(s) 907
Hsp92II CATG 8 cut(s) 616, 710, 741, 763, 824, 1010, 1016, 1037
HspAI GCGC 2 cut(s) 435, 784
KpnI GGTACC 1 cut(s) 1080
Ksp22I TGATCA 2 cut(s) 276, 915
KspAI GTTAAC 1 cut(s) 195
Kzo9I GATC 3 cut(s) 276, 915, 1140
Lsp1109I GCAGC 4 cut(s) 55, 162, 294, 496
LweI GCATC 1 cut(s) 464
MaeI CTAG 2 cut(s) 227, 333
MaeII ACGT 1 cut(s) 907
MaeIII GTNAC 2 cut(s) 646, 816
MalI GATC 3 cut(s) 278, 917, 1142
MboI GATC 3 cut(s) 276, 915, 1140
MflI RGATCY 1 cut(s) 1140
MhlI GDGCHC 4 cut(s) 113, 340, 913, 1131
MlsI TGGCCA 1 cut(s) 876
MluCI AATT 7 cut(s) 253, 401, 794, 878, 975, 992, 1022
MluNI TGGCCA 1 cut(s) 876
MlyI GAGTC 2 cut(s) 143, 412
MmeI TCCRAC 2 cut(s) 228, 874
MnlI CCTC 8 cut(s) 49, 52, 124, 182, 362, 628, 651, 1057
Mox20I TGGCCA 1 cut(s) 876
MroXI GAANNNNTTC 1 cut(s) 465
MscI TGGCCA 1 cut(s) 876
MseI TTAA 4 cut(s) 194, 360, 429, 827
MslI CAYNNNNRTG 4 cut(s) 611, 708, 1011, 1017
Msp20I TGGCCA 1 cut(s) 876
MspA1I CMGCKG 1 cut(s) 97
MspR9I CCNGG 1 cut(s) 641
Mva1269I GAATGC 1 cut(s) 770
MvaI CCWGG 1 cut(s) 641
MvnI CGCG 2 cut(s) 784, 1088
MwoI GCNNNNNNNGC 2 cut(s) 68, 740
NcoI CCATGG 2 cut(s) 706, 759
NdeII GATC 3 cut(s) 276, 915, 1140
NlaIII CATG 8 cut(s) 616, 710, 741, 763, 824, 1010, 1016, 1037
NlaIV GGNNCC 1 cut(s) 1078
NruI TCGCGA 1 cut(s) 1088
NspI RCATGY 1 cut(s) 741
OliI CACNNNNGTG 1 cut(s) 708
PaeR7I CTCGAG 1 cut(s) 128
PagI TCATGA 1 cut(s) 612
PctI GAATGC 1 cut(s) 770
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 3 cut(s) 77, 124, 947
PflMI CCANNNNNTGG 1 cut(s) 1116
PkrI GCNGC 6 cut(s) 9, 70, 152, 284, 511, 736
PleI GAGTC 2 cut(s) 142, 412
PpsI GAGTC 2 cut(s) 142, 412
PpuMI RGGWCCY 1 cut(s) 541
Psp124BI GAGCTC 2 cut(s) 113, 1131
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 1 cut(s) 639
PspFI CCCAGC 1 cut(s) 22
PspGI CCWGG 1 cut(s) 639
PspN4I GGNNCC 1 cut(s) 1078
PspPI GGNCC 2 cut(s) 541, 637
PspPPI RGGWCCY 1 cut(s) 541
PsrI GAACNNNNNNTAC 2 cut(s) 306, 338
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 1140
PvuII CAGCTG 1 cut(s) 97
RruI TCGCGA 1 cut(s) 1088
RsaI GTAC 2 cut(s) 676, 1078
RsaNI GTAC 2 cut(s) 675, 1077
RseI CAYNNNNRTG 4 cut(s) 611, 708, 1011, 1017
SacI GAGCTC 2 cut(s) 113, 1131
SaqAI TTAA 4 cut(s) 194, 360, 429, 827
SatI GCNGC 6 cut(s) 8, 69, 151, 283, 510, 735
Sau3AI GATC 3 cut(s) 276, 915, 1140
Sau96I GGNCC 2 cut(s) 541, 637
SchI GAGTC 2 cut(s) 143, 412
ScrFI CCNGG 1 cut(s) 641
SduI GDGCHC 4 cut(s) 113, 340, 913, 1131
SfaNI GCATC 1 cut(s) 464
SfcI CTRYAG 1 cut(s) 554
Sfr274I CTCGAG 1 cut(s) 128
SinI GGWCC 1 cut(s) 541
SlaI CTCGAG 1 cut(s) 128
SmiMI CAYNNNNRTG 4 cut(s) 611, 708, 1011, 1017
SmlI CTYRAG 2 cut(s) 128, 317
SmoI CTYRAG 2 cut(s) 128, 317
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 7 cut(s) 253, 401, 794, 878, 975, 992, 1022
SsiI CCGC 1 cut(s) 735
SspI AATATT 1 cut(s) 380
SspMI CTAG 2 cut(s) 227, 333
SstI GAGCTC 2 cut(s) 113, 1131
StyD4I CCNGG 1 cut(s) 639
StyI CCWWGG 2 cut(s) 706, 759
TaaI ACNGT 3 cut(s) 234, 610, 682
TaiI ACGT 1 cut(s) 910
TaqI TCGA 3 cut(s) 16, 129, 904
TasI AATT 7 cut(s) 253, 401, 794, 878, 975, 992, 1022
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 3 cut(s) 77, 124, 947
Tru1I TTAA 4 cut(s) 194, 360, 429, 827
Tru9I TTAA 4 cut(s) 194, 360, 429, 827
TscAI CASTG 4 cut(s) 249, 308, 613, 918
TseI GCWGC 5 cut(s) 7, 68, 150, 282, 509
TspDTI ATGAA 6 cut(s) 336, 744, 787, 1023, 1029, 1035
TspRI CASTG 4 cut(s) 249, 308, 613, 918
Van91I CCANNNNNTGG 1 cut(s) 1116
VneI GTGCAC 1 cut(s) 909
VpaK11BI GGWCC 1 cut(s) 541
XapI RAATTY 3 cut(s) 253, 794, 1022
XceI RCATGY 1 cut(s) 741
XhoI CTCGAG 1 cut(s) 128
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 2 cut(s) 227, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.