Rorug04G0288200

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0013447
Physical Location & Seq
Forward (+)
46125473 .. 46127200
1728 bp
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UTR
Exon/CDS
Intron
Rorug04G0288200.1

Sequence Viewer

Length: 828 bp
ATGGCGAGAAGAGCATGCACATCTCTTCCATTTCATGATGGTAGTGTTGAGGAGGAGAAGGGGCCTACCTTCTTCAAGATCATAAGGCCTGGATTCAACACTGAGCATTTGAGAATCCCACCTGCATTTCGGAAGCATATATTACATGAATCCACTAAGAGGGCAACCTTGAAATTGAAGAATTCATCAGATACTTCCTGGACTGTTAAAGTAAGTATAACAGGAAGGGATATATATTTGAAGGATGGCTGGCAGGAGTTTTTAAGAGAAAACTCCCTAGGTGATTCCGAGTTCTTAGTGTTTAGGTATGACAAAAATATGCATTTCATCATTAATATTTTTGACAAGAATTGCGTTGAGAGAGTGAATATCACCGACCTCAGAACACATAAAGCATCCACCTTTCCAGACAATACTAAAGGGCCTTTGGCAAGACCACCTACAGCTCCAGGTGATCATCGATATGACTCGGCTGTGGAAGCTGCTCATGTGCTTAAATCCAAAGGCGAGCACCTTCCAAGTTATGCAGCTGCTGCAGCTTTCAAATCCAAATTTCCTTACTTTGCAAAAGTTCTTGCAGGATCGAAGATGGTGGCAATTCCCACACACTTCTATAGGGAGATATTTTCTTCAAGCAAATATGATGAACTTTTCCTGGTGACTTCGAAGGGAAAATTCACAGTGACGCTTCTCCCATGCACTGATACAGTGATGTTGTCTGGAGGGTGGTCTTCCTTTAGAAATGACAACCAACTCCAGGAAGATGATATTTGCATCTTTGAGCTTGTGAAAGAAAACACCATGGTGGTTCACATTTTCCGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

275

Amino Acids

31.34

Weight (kDa)

9.05

Isoelectric Point (pI)

47.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 24 - 117 2.4e-17 B3 DNA binding domain
B3 PF02362 195 - 275 1.1e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13310
fragaria_vesca FvH4_4g27970
malus_domestica MD13G1045500.v1.1
prunus_persica Prupe.1G306500_v2.0.a1
pyrus_communis pycom13g04000
rosa_chinensis RchiOBHm_Chr4g0435681
rosa_laevigata RLG00000006567
rosa_multiflora Rmu_sc0000051.1_g000013
rosa_roxburghii Rroxscaffold_5G00376890
rosa_rugosa Rorug04G0288200 Rorug04G0288300 Rorug04G0288400 Rorug04G0288500
rosa_samantha Rh4AG343100 Rh4BG351500 Rh4CG366100 Rh4DG345700
rosa_wichuraiana Rw4G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 130
AclWI GGATC 1 cut(s) 589
AcsI RAATTY 3 cut(s) 181, 551, 674
AfiI CCNNNNNNNGG 2 cut(s) 159, 757
AgsI TTSAA 7 cut(s) 76, 97, 172, 178, 241, 544, 633
AjnI CCWGG 5 cut(s) 88, 197, 448, 654, 756
AleI CACNNNNGTG 1 cut(s) 803
AluBI AGCT 5 cut(s) 446, 482, 530, 539, 784
AluI AGCT 5 cut(s) 446, 482, 530, 539, 784
Alw21I GWGCWC 1 cut(s) 513
AlwI GGATC 1 cut(s) 589
AlwNI CAGNNNCTG 1 cut(s) 533
AoxI GGCC 3 cut(s) 62, 86, 422
ApeKI GCWGC 5 cut(s) 482, 527, 530, 533, 536
ApoI RAATTY 3 cut(s) 181, 551, 674
AseI ATTAAT 1 cut(s) 333
AspA2I CCTAGG 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 62, 422
AsuHPI GGTGA 4 cut(s) 293, 364, 464, 670
AsuII TTCGAA 1 cut(s) 665
AvrII CCTAGG 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 5 cut(s) 469, 517, 520, 539, 548
BccI CCATC 3 cut(s) 32, 239, 583
BciT130I CCWGG 5 cut(s) 90, 199, 450, 656, 758
BclI TGATCA 1 cut(s) 454
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 3 cut(s) 441, 534, 613
BfuAI ACCTGC 1 cut(s) 130
BisI GCNGC 5 cut(s) 483, 528, 531, 534, 537
BlnI CCTAGG 1 cut(s) 277
BlsI GCNGC 5 cut(s) 484, 529, 532, 535, 538
Bme1390I CCNGG 5 cut(s) 90, 199, 450, 656, 758
BmgT120I GGNCC 2 cut(s) 62, 422
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 5 cut(s) 90, 199, 450, 656, 758
BmsI GCATC 2 cut(s) 404, 783
BpiI GAAGAC 1 cut(s) 723
BpmI CTGGAG 3 cut(s) 432, 740, 741
Bpu14I TTCGAA 1 cut(s) 665
Bsa29I ATCGAT 1 cut(s) 460
BsaJI CCNNGG 2 cut(s) 277, 801
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 757
BseBI CCWGG 5 cut(s) 90, 199, 450, 656, 758
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 2 cut(s) 277, 801
BseGI GGATG 2 cut(s) 250, 395
BseLI CCNNNNNNNGG 2 cut(s) 159, 757
BseMII CTCAG 2 cut(s) 93, 394
BseRI GAGGAG 2 cut(s) 65, 68
BseXI GCAGC 5 cut(s) 469, 517, 520, 539, 548
BshFI GGCC 3 cut(s) 64, 88, 424
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 513
BslI CCNNNNNNNGG 2 cut(s) 159, 757
BsnI GGCC 3 cut(s) 64, 88, 424
Bsp119I TTCGAA 1 cut(s) 665
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 3 cut(s) 78, 454, 581
Bsp19I CCATGG 1 cut(s) 801
BspANI GGCC 3 cut(s) 64, 88, 424
BspCNI CTCAG 2 cut(s) 94, 393
BspDI ATCGAT 1 cut(s) 460
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 538
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 1 cut(s) 589
BspQI GCTCTTC 1 cut(s) 4
BspT104I TTCGAA 1 cut(s) 665
BssECI CCNNGG 2 cut(s) 277, 801
BssMI GATC 3 cut(s) 78, 454, 581
BssT1I CCWWGG 2 cut(s) 277, 801
Bst2UI CCWGG 5 cut(s) 90, 199, 450, 656, 758
Bst4CI ACNGT 3 cut(s) 205, 682, 709
Bst6I CTCTTC 2 cut(s) 4, 30
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 665
BstC8I GCNNGC 3 cut(s) 16, 251, 509
BstDEI CTNAG 4 cut(s) 102, 156, 295, 380
BstDSI CCRYGG 1 cut(s) 801
BstF5I GGATG 2 cut(s) 250, 395
BstKTI GATC 3 cut(s) 81, 457, 584
BstMBI GATC 3 cut(s) 78, 454, 581
BstMWI GCNNNNNNNGC 4 cut(s) 11, 479, 533, 536
BstNI CCWGG 5 cut(s) 90, 199, 450, 656, 758
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 5 cut(s) 88, 197, 448, 654, 756
BstSFI CTRYAG 3 cut(s) 441, 534, 613
BstV1I GCAGC 5 cut(s) 469, 517, 520, 539, 548
BstV2I GAAGAC 1 cut(s) 723
Bsu15I ATCGAT 1 cut(s) 460
BsuRI GGCC 3 cut(s) 64, 88, 424
BsuTUI ATCGAT 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 801
BtsCI GGATG 2 cut(s) 250, 395
BtsIMutI CAGTG 4 cut(s) 99, 687, 699, 714
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 3 cut(s) 16, 251, 509
CaiI CAGNNNCTG 1 cut(s) 533
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 62, 422
ClaI ATCGAT 1 cut(s) 460
CseI GACGC 1 cut(s) 694
CviAII CATG 6 cut(s) 15, 35, 146, 488, 696, 802
DdeI CTNAG 4 cut(s) 102, 156, 295, 380
DpnI GATC 3 cut(s) 80, 456, 583
DpnII GATC 3 cut(s) 78, 454, 581
Eam1104I CTCTTC 2 cut(s) 4, 30
EarI CTCTTC 2 cut(s) 4, 30
Eco130I CCWWGG 2 cut(s) 277, 801
Eco147I AGGCCT 1 cut(s) 88
EcoO109I RGGNCCY 2 cut(s) 62, 422
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 5 cut(s) 88, 197, 448, 654, 756
EcoT14I CCWWGG 2 cut(s) 277, 801
EcoT22I ATGCAT 1 cut(s) 324
ErhI CCWWGG 2 cut(s) 277, 801
FaeI CATG 6 cut(s) 18, 38, 149, 491, 699, 805
FatI CATG 6 cut(s) 14, 34, 145, 487, 695, 801
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 5 cut(s) 483, 528, 531, 534, 537
FokI GGATG 2 cut(s) 257, 382
Fsp4HI GCNGC 5 cut(s) 483, 528, 531, 534, 537
FspBI CTAG 1 cut(s) 278
GluI GCNGC 5 cut(s) 483, 528, 531, 534, 537
GsuI CTGGAG 3 cut(s) 432, 740, 741
HaeIII GGCC 3 cut(s) 64, 88, 424
HgaI GACGC 1 cut(s) 694
Hin1II CATG 6 cut(s) 18, 38, 149, 491, 699, 805
HinfI GANTC 5 cut(s) 93, 114, 149, 284, 467
HphI GGTGA 4 cut(s) 293, 364, 464, 670
Hpy166II GTNNAC 1 cut(s) 811
Hpy188I TCNGA 5 cut(s) 132, 190, 289, 383, 821
Hpy188III TCNNGA 4 cut(s) 35, 76, 407, 720
Hpy8I GTNNAC 1 cut(s) 811
HpyAV CCTTC 6 cut(s) 52, 79, 219, 235, 524, 661
HpyCH4III ACNGT 3 cut(s) 205, 682, 709
HpyCH4V TGCA 9 cut(s) 18, 125, 322, 527, 536, 566, 578, 699, 774
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 479, 533, 536
HpyF3I CTNAG 4 cut(s) 102, 156, 295, 380
Hsp92II CATG 6 cut(s) 18, 38, 149, 491, 699, 805
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 3 cut(s) 78, 454, 581
LguI GCTCTTC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 451
Lsp1109I GCAGC 5 cut(s) 469, 517, 520, 539, 548
LweI GCATC 2 cut(s) 404, 783
MaeI CTAG 1 cut(s) 278
MaeIII GTNAC 2 cut(s) 658, 682
MalI GATC 3 cut(s) 80, 456, 583
MboI GATC 3 cut(s) 78, 454, 581
MboII GAAGA 8 cut(s) 17, 21, 64, 190, 598, 621, 723, 773
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 6 cut(s) 173, 181, 349, 551, 597, 674
MlyI GAGTC 1 cut(s) 461
MnlI CCTC 5 cut(s) 43, 46, 153, 389, 716
Mph1103I ATGCAT 1 cut(s) 324
MseI TTAA 4 cut(s) 207, 263, 333, 495
MslI CAYNNNNRTG 2 cut(s) 462, 803
MspA1I CMGCKG 1 cut(s) 530
MspR9I CCNGG 5 cut(s) 90, 199, 450, 656, 758
MvaI CCWGG 5 cut(s) 90, 199, 450, 656, 758
MwoI GCNNNNNNNGC 4 cut(s) 11, 479, 533, 536
NcoI CCATGG 1 cut(s) 801
NdeII GATC 3 cut(s) 78, 454, 581
NlaIII CATG 6 cut(s) 18, 38, 149, 491, 699, 805
NlaIV GGNNCC 1 cut(s) 63
NmeAIII GCCGAG 1 cut(s) 449
NmuCI GTSAC 2 cut(s) 658, 682
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 1 cut(s) 18
NspV TTCGAA 1 cut(s) 665
OliI CACNNNNGTG 1 cut(s) 803
PaeI GCATGC 1 cut(s) 18
PagI TCATGA 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 130
PceI AGGCCT 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 4
PfeI GAWTC 4 cut(s) 93, 114, 149, 284
PfoI TCCNGGA 2 cut(s) 197, 756
PkrI GCNGC 5 cut(s) 484, 529, 532, 535, 538
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
PshBI ATTAAT 1 cut(s) 333
Psp6I CCWGG 5 cut(s) 88, 197, 448, 654, 756
PspGI CCWGG 5 cut(s) 88, 197, 448, 654, 756
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 2 cut(s) 62, 422
PstI CTGCAG 1 cut(s) 538
PstNI CAGNNNCTG 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 530
RseI CAYNNNNRTG 2 cut(s) 462, 803
SapI GCTCTTC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 207, 263, 333, 495
SatI GCNGC 5 cut(s) 483, 528, 531, 534, 537
Sau3AI GATC 3 cut(s) 78, 454, 581
Sau96I GGNCC 2 cut(s) 62, 422
SchI GAGTC 1 cut(s) 461
ScrFI CCNGG 5 cut(s) 90, 199, 450, 656, 758
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 2 cut(s) 404, 783
SfcI CTRYAG 3 cut(s) 441, 534, 613
SfuI TTCGAA 1 cut(s) 665
SmiMI CAYNNNNRTG 2 cut(s) 462, 803
SphI GCATGC 1 cut(s) 18
Sse9I AATT 6 cut(s) 173, 181, 349, 551, 597, 674
SseBI AGGCCT 1 cut(s) 88
SspI AATATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 278
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 5 cut(s) 88, 197, 448, 654, 756
StyI CCWWGG 2 cut(s) 277, 801
TaaI ACNGT 3 cut(s) 205, 682, 709
TaqI TCGA 3 cut(s) 460, 584, 665
TasI AATT 6 cut(s) 173, 181, 349, 551, 597, 674
TfiI GAWTC 4 cut(s) 93, 114, 149, 284
Tru1I TTAA 4 cut(s) 207, 263, 333, 495
Tru9I TTAA 4 cut(s) 207, 263, 333, 495
TscAI CASTG 4 cut(s) 106, 687, 706, 714
TseFI GTSAC 2 cut(s) 658, 682
TseI GCWGC 5 cut(s) 482, 527, 530, 533, 536
Tsp45I GTSAC 2 cut(s) 658, 682
TspDTI ATGAA 5 cut(s) 23, 162, 174, 316, 660
TspRI CASTG 4 cut(s) 106, 687, 706, 714
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 3 cut(s) 181, 551, 674
XceI RCATGY 1 cut(s) 18
XmaJI CCTAGG 1 cut(s) 277
XspI CTAG 1 cut(s) 278
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.