MD13G1045500.v1.1

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0013447
Physical Location & Seq
Forward (+)
3149475 .. 3152208
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1045500.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGATGCTGCACTTTAATGACCCGGCAAAGTTGAAGACCAAGAAGATTGTGTTCGAGGAGGTTTACGCTGCTCGTGATTCCGCGACACTTGAGCAACTGAAAGAATTGAGCTCTAAGCGCAGAATGCTTGAGGAGTCCATCAATGAGAGCAGCTCCATCAATGGCGCCATTGCAAGAGAAATGTCCGGAGGGCTAACTTCTCGTAATCAACAGGTTCTTCACAACCTTGAACTGTATCTTCCCTTGTTGGAAAATTTGATTTGGCACGTTGATCGGGTCAAGAGCCACCACCAGATAGTTCGATGGACTTCAGAACTTAAGATACAATGGACTAGTGTTCTTAGTTCATCTTCTTTCTTTAACTTGAAAGGATCGAAATATTTTCAAATCAACAACTTGCAGTTTGAGTTGTGCCTGACCCTTTTCCTCTATGGCGCAATCCTTCGGGAGAGGGCATTAGAAGTTCTTCCTGCAGACCTAGTGCAATCTGCCACGCTATTCCGAGAAGCTGCTGGTGTTTTTCATCACCTTGCTCATCAAGTTATTCCTTTTCTACAACACTCATTGGTTGGGGAAAGGCCACCAGAAGTTGTCTCATCAGTGTCTGCTGCCATGAGCCTCATTTGCTTGGCCGATGCCCAGGCCGTAACAATTAAAAGGGCTGAAGAGAAGGGTACTACTGTCGGTCTTTTGGCAAAGTTGCACCATGGTATTGTAGAATTGCTTGATGAAGCTGCAGGCCTTTTGCATACGGCAAAGGAATGTAAAGACACTTCTTCGGATGTTGCGGACTTTTTATCATCCTGCAAAATGCTACACGAGTTACAAGCCCAGAAATACTTTGCGGAAACCCTAAAGCGTTCCAACCAATTCGGGGTCGCTGTTGCAGTTCTTCGATTCACACTGACTAATGTGAAAAAGAACATGCCCGGCAAAGAATCGTGGAAGTCTGTTCTCAAAGAGGAAATTGACAATTTTTCCGAATTGCTCAGAAAGCATGAACATGAAAACGAATTTGTTTGGCACGAGAAAATCCCCTCGGAGGAAATGTTGTTGCCAAAACCCGAAGGTAACAAAATCGCAAACATCATCCCTTATCAACCGAAAAAATGGGAAAGACAGCTTGCCTTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

380

Amino Acids

43.09

Weight (kDa)

7.29

Isoelectric Point (pI)

41.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BRO1 PF03097 104 - 366 2e-27 BRO1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13310
fragaria_vesca FvH4_4g27970
malus_domestica MD13G1045500.v1.1
prunus_persica Prupe.1G306500_v2.0.a1
pyrus_communis pycom13g04000
rosa_chinensis RchiOBHm_Chr4g0435681
rosa_laevigata RLG00000006567
rosa_multiflora Rmu_sc0000051.1_g000013
rosa_roxburghii Rroxscaffold_5G00376890
rosa_rugosa Rorug04G0288200 Rorug04G0288300 Rorug04G0288400 Rorug04G0288500
rosa_samantha Rh4AG343100 Rh4BG351500 Rh4CG366100 Rh4DG345700
rosa_wichuraiana Rw4G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 164
AccII CGCG 1 cut(s) 83
AccIII TCCGGA 1 cut(s) 185
AciI CCGC 3 cut(s) 81, 788, 845
AclWI GGATC 1 cut(s) 379
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 2 cut(s) 253, 1013
AcuI CTGAAG 2 cut(s) 294, 684
AcyI GRCGYC 1 cut(s) 165
AfaI GTAC 1 cut(s) 676
AfiI CCNNNNNNNGG 2 cut(s) 874, 1042
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 5 cut(s) 34, 230, 367, 386, 1132
AhlI ACTAGT 1 cut(s) 332
AjnI CCWGG 1 cut(s) 639
AluBI AGCT 5 cut(s) 111, 153, 509, 734, 1123
AluI AGCT 5 cut(s) 111, 153, 509, 734, 1123
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 598
AlwI GGATC 1 cut(s) 379
AlwNI CAGNNNCTG 1 cut(s) 605
Aor13HI TCCGGA 1 cut(s) 185
AoxI GGCC 4 cut(s) 578, 630, 642, 739
ApeKI GCWGC 6 cut(s) 7, 68, 150, 509, 608, 734
ApoI RAATTY 2 cut(s) 253, 1013
Asp700I GAANNNNTTC 1 cut(s) 465
AspLEI GCGC 3 cut(s) 120, 167, 437
AsuC2I CCSGG 2 cut(s) 23, 930
AsuHPI GGTGA 1 cut(s) 518
BaeI ACNNNNGTAYC 2 cut(s) 666, 699
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 113
BarI GAAGNNNNNNTAC 2 cut(s) 757, 789
BauI CACGAG 3 cut(s) 72, 818, 1025
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 113
BbvI GCAGC 5 cut(s) 55, 162, 496, 595, 721
BccI CCATC 3 cut(s) 146, 164, 297
BceAI ACGGC 2 cut(s) 629, 768
BcgI CGANNNNNNTGC 2 cut(s) 254, 288
BciT130I CCWGG 1 cut(s) 641
BcnI CCSGG 2 cut(s) 23, 930
BcoDI GTCTC 1 cut(s) 598
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 2 cut(s) 333, 479
BfmI CTRYAG 2 cut(s) 471, 735
BfoI RGCGCY 1 cut(s) 168
BfrI CTTAAG 1 cut(s) 317
BisI GCNGC 6 cut(s) 8, 69, 151, 510, 609, 735
BlsI GCNGC 6 cut(s) 9, 70, 152, 511, 610, 736
Bme1390I CCNGG 3 cut(s) 23, 641, 930
BmiI GGNNCC 1 cut(s) 166
BmrFI CCNGG 3 cut(s) 23, 641, 930
BmsI GCATC 1 cut(s) 625
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpuEI CTTGAG 2 cut(s) 110, 149
BpuMI CCSGG 2 cut(s) 23, 930
BsaHI GRCGYC 1 cut(s) 165
BsaJI CCNNGG 3 cut(s) 639, 706, 1038
BsaWI WCCGGW 1 cut(s) 185
BsaXI ACNNNNNCTCC 2 cut(s) 1034, 1064
Bsc4I CCNNNNNNNGG 2 cut(s) 874, 1042
Bse3DI GCAATG 1 cut(s) 168
BseAI TCCGGA 1 cut(s) 185
BseBI CCWGG 1 cut(s) 641
BseDI CCNNGG 3 cut(s) 639, 706, 1038
BseGI GGATG 3 cut(s) 787, 800, 1089
BseLI CCNNNNNNNGG 2 cut(s) 874, 1042
BseMI GCAATG 1 cut(s) 168
BseMII CTCAG 1 cut(s) 1003
BseRI GAGGAG 2 cut(s) 71, 146
BseXI GCAGC 5 cut(s) 55, 162, 496, 595, 721
Bsh1236I CGCG 1 cut(s) 83
BshFI GGCC 4 cut(s) 580, 632, 644, 741
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 113
BsiSI CCGG 3 cut(s) 23, 186, 930
BslI CCNNNNNNNGG 2 cut(s) 874, 1042
BsmAI GTCTC 1 cut(s) 598
BsmI GAATGC 1 cut(s) 129
BsnI GGCC 4 cut(s) 580, 632, 644, 741
Bsp1286I GDGCHC 1 cut(s) 113
Bsp13I TCCGGA 1 cut(s) 185
Bsp143I GATC 2 cut(s) 271, 371
Bsp19I CCATGG 1 cut(s) 706
BspACI CCGC 3 cut(s) 81, 788, 845
BspANI GGCC 4 cut(s) 580, 632, 644, 741
BspCNI CTCAG 1 cut(s) 1002
BspEI TCCGGA 1 cut(s) 185
BspFNI CGCG 1 cut(s) 83
BspLI GGNNCC 1 cut(s) 166
BspMAI CTGCAG 2 cut(s) 475, 739
BspPI GGATC 1 cut(s) 379
BspT107I GGYRCC 1 cut(s) 164
BspTI CTTAAG 1 cut(s) 317
BsrDI GCAATG 1 cut(s) 168
BssECI CCNNGG 3 cut(s) 639, 706, 1038
BssMI GATC 2 cut(s) 271, 371
BssNI GRCGYC 1 cut(s) 165
BssSI CACGAG 3 cut(s) 72, 818, 1025
BssT1I CCWWGG 1 cut(s) 706
Bst2BI CACGAG 3 cut(s) 72, 818, 1025
Bst2UI CCWGG 1 cut(s) 641
Bst4CI ACNGT 2 cut(s) 234, 682
Bst6I CTCTTC 1 cut(s) 660
BstACI GRCGYC 1 cut(s) 165
BstAFI CTTAAG 1 cut(s) 317
BstC8I GCNNGC 2 cut(s) 739, 1125
BstDEI CTNAG 3 cut(s) 114, 341, 989
BstDSI CCRYGG 1 cut(s) 706
BstF5I GGATG 3 cut(s) 787, 800, 1089
BstFNI CGCG 1 cut(s) 83
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 3 cut(s) 120, 167, 437
BstKTI GATC 2 cut(s) 274, 374
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 2 cut(s) 271, 371
BstMWI GCNNNNNNNGC 4 cut(s) 117, 124, 624, 994
BstNI CCWGG 1 cut(s) 641
BstNSI RCATGY 1 cut(s) 928
BstSCI CCNGG 3 cut(s) 21, 639, 928
BstSFI CTRYAG 2 cut(s) 471, 735
BstUI CGCG 1 cut(s) 83
BstV1I GCAGC 5 cut(s) 55, 162, 496, 595, 721
BstV2I GAAGAC 1 cut(s) 41
BsuRI GGCC 4 cut(s) 580, 632, 644, 741
BtgI CCRYGG 1 cut(s) 706
BtsCI GGATG 3 cut(s) 787, 800, 1089
BtsIMutI CAGTG 2 cut(s) 606, 902
Cac8I GCNNGC 2 cut(s) 739, 1125
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 3 cut(s) 120, 167, 437
Csp6I GTAC 1 cut(s) 675
CviAII CATG 5 cut(s) 613, 707, 925, 998, 1004
CviQI GTAC 1 cut(s) 675
DdeI CTNAG 3 cut(s) 114, 341, 989
DinI GGCGCC 1 cut(s) 166
DpnI GATC 2 cut(s) 273, 373
DpnII GATC 2 cut(s) 271, 371
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 1 cut(s) 660
EarI CTCTTC 1 cut(s) 660
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 706
Eco147I AGGCCT 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 113
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 2 cut(s) 294, 684
EcoICRI GAGCTC 1 cut(s) 111
EcoRII CCWGG 1 cut(s) 639
EcoT14I CCWWGG 1 cut(s) 706
EcoT38I GRGCYC 1 cut(s) 113
EgeI GGCGCC 1 cut(s) 166
EheI GGCGCC 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 706
FaeI CATG 5 cut(s) 616, 710, 928, 1001, 1007
FaiI YATR 7 cut(s) 432, 614, 708, 750, 926, 999, 1005
FatI CATG 5 cut(s) 612, 706, 924, 997, 1003
Fnu4HI GCNGC 6 cut(s) 8, 69, 151, 510, 609, 735
FokI GGATG 3 cut(s) 787, 794, 1076
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 6 cut(s) 8, 69, 151, 510, 609, 735
FspBI CTAG 2 cut(s) 333, 479
GlaI GCGC 3 cut(s) 119, 166, 436
GluI GCNGC 6 cut(s) 8, 69, 151, 510, 609, 735
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 4 cut(s) 580, 632, 644, 741
HapII CCGG 3 cut(s) 23, 186, 930
HhaI GCGC 3 cut(s) 120, 167, 437
Hin1I GRCGYC 1 cut(s) 165
Hin1II CATG 5 cut(s) 616, 710, 928, 1001, 1007
Hin6I GCGC 3 cut(s) 118, 165, 435
HinP1I GCGC 3 cut(s) 118, 165, 435
HinfI GANTC 4 cut(s) 77, 134, 897, 938
HpaII CCGG 3 cut(s) 23, 186, 930
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 6 cut(s) 313, 503, 781, 982, 992, 1042
Hpy188III TCNNGA 5 cut(s) 74, 186, 280, 446, 1132
Hpy8I GTNNAC 1 cut(s) 64
HpyAV CCTTC 4 cut(s) 452, 664, 1061, 1138
HpyCH4III ACNGT 2 cut(s) 234, 682
HpyCH4IV ACGT 1 cut(s) 267
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 124, 624, 994
HpyF3I CTNAG 3 cut(s) 114, 341, 989
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 165
Hsp92II CATG 5 cut(s) 616, 710, 928, 1001, 1007
HspAI GCGC 3 cut(s) 118, 165, 435
KasI GGCGCC 1 cut(s) 164
Kpn2I TCCGGA 1 cut(s) 185
Kzo9I GATC 2 cut(s) 271, 371
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 5 cut(s) 55, 162, 496, 595, 721
LweI GCATC 1 cut(s) 625
MaeI CTAG 2 cut(s) 333, 479
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 3 cut(s) 646, 822, 1070
MalI GATC 2 cut(s) 273, 373
MboI GATC 2 cut(s) 271, 371
MboII GAAGA 9 cut(s) 46, 55, 209, 230, 342, 458, 677, 768, 884
MhlI GDGCHC 1 cut(s) 113
MluCI AATT 9 cut(s) 104, 253, 651, 719, 869, 966, 973, 983, 1013
Mly113I GGCGCC 1 cut(s) 165
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 2 cut(s) 228, 888
MroI TCCGGA 1 cut(s) 185
MroXI GAANNNNTTC 1 cut(s) 465
MseI TTAA 4 cut(s) 15, 318, 360, 654
MslI CAYNNNNRTG 2 cut(s) 15, 1002
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 3 cut(s) 23, 186, 930
MspR9I CCNGG 3 cut(s) 23, 641, 930
Mva1269I GAATGC 1 cut(s) 129
MvaI CCWGG 1 cut(s) 641
MvnI CGCG 1 cut(s) 83
MwoI GCNNNNNNNGC 4 cut(s) 117, 124, 624, 994
NarI GGCGCC 1 cut(s) 165
NciI CCSGG 2 cut(s) 23, 930
NcoI CCATGG 1 cut(s) 706
NdeII GATC 2 cut(s) 271, 371
NlaIII CATG 5 cut(s) 616, 710, 928, 1001, 1007
NlaIV GGNNCC 1 cut(s) 166
NspI RCATGY 1 cut(s) 928
PceI AGGCCT 1 cut(s) 741
PctI GAATGC 1 cut(s) 129
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 3 cut(s) 77, 897, 938
PkrI GCNGC 6 cut(s) 9, 70, 152, 511, 610, 736
PleI GAGTC 1 cut(s) 142
PluTI GGCGCC 1 cut(s) 168
PpsI GAGTC 1 cut(s) 142
Psp124BI GAGCTC 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 639
PspGI CCWGG 1 cut(s) 639
PspN4I GGNNCC 1 cut(s) 166
PsrI GAACNNNNNNTAC 2 cut(s) 306, 338
PstI CTGCAG 2 cut(s) 475, 739
PstNI CAGNNNCTG 1 cut(s) 605
RsaI GTAC 1 cut(s) 676
RsaNI GTAC 1 cut(s) 675
RseI CAYNNNNRTG 2 cut(s) 15, 1002
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 4 cut(s) 15, 318, 360, 654
SatI GCNGC 6 cut(s) 8, 69, 151, 510, 609, 735
Sau3AI GATC 2 cut(s) 271, 371
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 3 cut(s) 23, 641, 930
SduI GDGCHC 1 cut(s) 113
SfaNI GCATC 1 cut(s) 625
SfcI CTRYAG 2 cut(s) 471, 735
SfoI GGCGCC 1 cut(s) 166
SmiMI CAYNNNNRTG 2 cut(s) 15, 1002
SmlI CTYRAG 3 cut(s) 89, 128, 317
SmoI CTYRAG 3 cut(s) 89, 128, 317
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 9 cut(s) 104, 253, 651, 719, 869, 966, 973, 983, 1013
SseBI AGGCCT 1 cut(s) 741
SsiI CCGC 3 cut(s) 81, 788, 845
SspDI GGCGCC 1 cut(s) 164
SspI AATATT 1 cut(s) 380
SspMI CTAG 2 cut(s) 333, 479
SstI GAGCTC 1 cut(s) 113
StuI AGGCCT 1 cut(s) 741
StyD4I CCNGG 3 cut(s) 21, 639, 928
StyI CCWWGG 1 cut(s) 706
TaaI ACNGT 2 cut(s) 234, 682
TaiI ACGT 1 cut(s) 270
TaqI TCGA 4 cut(s) 54, 301, 374, 895
TaqII GACCGA 1 cut(s) 674
TasI AATT 9 cut(s) 104, 253, 651, 719, 869, 966, 973, 983, 1013
TfiI GAWTC 3 cut(s) 77, 897, 938
Tru1I TTAA 4 cut(s) 15, 318, 360, 654
Tru9I TTAA 4 cut(s) 15, 318, 360, 654
TscAI CASTG 2 cut(s) 606, 909
TseI GCWGC 6 cut(s) 7, 68, 150, 509, 608, 734
TspDTI ATGAA 5 cut(s) 336, 512, 744, 1014, 1020
TspRI CASTG 2 cut(s) 606, 909
Vha464I CTTAAG 1 cut(s) 317
XapI RAATTY 2 cut(s) 253, 1013
XceI RCATGY 1 cut(s) 928
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 2 cut(s) 333, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.