Prupe.1G306500_v2.0.a1

BRO1-like domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0013447
Physical Location & Seq
Reverse (-)
29953649 .. 29956739
3091 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G306500.1

Sequence Viewer

Length: 1143 bp
ATGATGCTTCATTTTCGAGACCCGGCAAAGTTGAAGACTAAGAAGATTGTGTTTGAAGAGGCTTATGCTGCTCGTGATTCCACAACACTTGAGCAACTGAAAGAATTAAGCTCTAAGCGTAGAATGATTGAGGAGTCTATTAATGAGAGCAGCTCCATTACTACGGCCATTGCAAGAGAAATGTCTGGAGGGTTAACTTCTCGTTCTCAACAGGTTCTTCACAACCTAGAAATGTATCTTCCATTGCTGGAGAATTTGATTTTCCATGTGGGCATGATCGGCGACTTCGGCCAGATAGTTCGATGGAATTCAGAACTTAAGATGCAATGGACTAGTGCTCTTAGTTCATCTTCTTTCTTTAACTTTAAAGGTCCAAAATATTTTCAAATCAATAGCTTGCAGTTTGAGGTTGGTATGACCCTTTTCCTTTATGGTGCAATCCTTCGGGAGAGGGCATTAGAAGTTCTTCCTGCAGACCTAGTGCAATCGGCCACCCTTTTCAGAGAAGCTGCTGGTGTTTTTCATCATCTTGCTCATAAGGTTCTTCCTATTCTACAATATTCACTCACTGCGGAAAGGCCACCAGAAGTTGTCTCATCTGTGTCCACTGTCATGAGCCTCATTTGTTTGGCCGAGGCCCAGGCGGTAACAATAAAGAGGGCTGAAGAGAAGGGCACTACCGTGGGTCTTTTGGCAAAGTTGCATCATGGTGTTGCAGAGTTGCTTGATGAAGCTGCAGGTGTTTTAAATACAGCAAGTAGGGAATGTAATGATATTTCTTCACGCATTGTGGAATTCATATCATGTTGCAAAGCGCTACATGAGTTAAAAAGTAAGAAATACTTTGCCGAAAGCCTGAAGATTGCCAACCAATTCGGGGCTGCCATCGGAGTTCTTCGAGTTGCACTGGTTGATGTGAAGAAGAAGAAAATGCCAGGCCAAGAGTCATGGAAATATGTTCTCAAAGAGGAAATTGATAAGTTTGCGGAATTACTCAGGAAGCATGAACATGAAAACGAGTTTGTTTGGCATGAGAAAGTCCCCTCTGAGGATGAGTTGCCAAAACCTGAAGGTAACAAAATTGCAAGTATCATACCTTATCAACCCAAAAGATGGGAAAGACAACTTGCCTTCAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BRO1

Protein Analysis

381

Amino Acids

42.99

Weight (kDa)

8.19

Isoelectric Point (pI)

47.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013447)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13310
fragaria_vesca FvH4_4g27970
malus_domestica MD13G1045500.v1.1
prunus_persica Prupe.1G306500_v2.0.a1
pyrus_communis pycom13g04000
rosa_chinensis RchiOBHm_Chr4g0435681
rosa_laevigata RLG00000006567
rosa_multiflora Rmu_sc0000051.1_g000013
rosa_roxburghii Rroxscaffold_5G00376890
rosa_rugosa Rorug04G0288200 Rorug04G0288300 Rorug04G0288400 Rorug04G0288500
rosa_samantha Rh4AG343100 Rh4BG351500 Rh4CG366100 Rh4DG345700
rosa_wichuraiana Rw4G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 728
Acc36I ACCTGC 1 cut(s) 728
AccB7I CCANNNNNTGG 1 cut(s) 1113
AciI CCGC 3 cut(s) 572, 644, 986
AcoI YGGCCR 4 cut(s) 165, 289, 489, 630
AcsI RAATTY 3 cut(s) 253, 307, 794
AcuI CTGAAG 3 cut(s) 684, 878, 1089
AfeI AGCGCT 1 cut(s) 816
AfiI CCNNNNNNNGG 3 cut(s) 877, 1048, 1113
AflII CTTAAG 1 cut(s) 317
AgsI TTSAA 4 cut(s) 34, 56, 386, 1135
AhlI ACTAGT 1 cut(s) 332
AjnI CCWGG 2 cut(s) 639, 934
AleI CACNNNNGTG 1 cut(s) 680
AluBI AGCT 5 cut(s) 111, 153, 396, 509, 734
AluI AGCT 5 cut(s) 111, 153, 396, 509, 734
Alw21I GWGCWC 1 cut(s) 340
Alw26I GTCTC 2 cut(s) 12, 598
Aor51HI AGCGCT 1 cut(s) 816
AoxI GGCC 7 cut(s) 165, 289, 489, 578, 630, 636, 937
ApeKI GCWGC 5 cut(s) 68, 150, 509, 734, 881
ApoI RAATTY 3 cut(s) 253, 307, 794
AseI ATTAAT 1 cut(s) 141
Asp700I GAANNNNTTC 1 cut(s) 465
AspLEI GCGC 1 cut(s) 817
AspS9I GGNCC 2 cut(s) 371, 637
AsuC2I CCSGG 1 cut(s) 23
AvaII GGWCC 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 677
BauI CACGAG 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 5 cut(s) 55, 162, 496, 721, 868
BccI CCATC 3 cut(s) 297, 893, 1107
BceAI ACGGC 1 cut(s) 180
BciT130I CCWGG 2 cut(s) 641, 936
BcnI CCSGG 1 cut(s) 23
BcoDI GTCTC 2 cut(s) 12, 598
BcuI ACTAGT 1 cut(s) 332
BfaI CTAG 3 cut(s) 227, 333, 479
BfmI CTRYAG 2 cut(s) 471, 735
BfoI RGCGCY 1 cut(s) 818
BfrI CTTAAG 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 728
BisI GCNGC 5 cut(s) 69, 151, 510, 735, 882
BlsI GCNGC 5 cut(s) 70, 152, 511, 736, 883
Bme1390I CCNGG 3 cut(s) 23, 641, 936
Bme18I GGWCC 1 cut(s) 371
BmgT120I GGNCC 2 cut(s) 371, 637
BmrFI CCNGG 3 cut(s) 23, 641, 936
BmsI GCATC 2 cut(s) 312, 712
BpiI GAAGAC 1 cut(s) 41
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 2 cut(s) 207, 269
BpuEI CTTGAG 1 cut(s) 110
BpuMI CCSGG 1 cut(s) 23
BsaI GGTCTC 1 cut(s) 12
BsaJI CCNNGG 3 cut(s) 633, 639, 681
Bsc4I CCNNNNNNNGG 3 cut(s) 877, 1048, 1113
Bse1I ACTGG 1 cut(s) 912
Bse3DI GCAATG 3 cut(s) 168, 242, 332
BseBI CCWGG 2 cut(s) 641, 936
BseDI CCNNGG 3 cut(s) 633, 639, 681
BseGI GGATG 1 cut(s) 1057
BseLI CCNNNNNNNGG 3 cut(s) 877, 1048, 1113
BseMI GCAATG 3 cut(s) 168, 242, 332
BseMII CTCAG 2 cut(s) 1009, 1038
BseNI ACTGG 1 cut(s) 912
BseRI GAGGAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 677
BseXI GCAGC 5 cut(s) 55, 162, 496, 721, 868
BshFI GGCC 7 cut(s) 167, 291, 491, 580, 632, 638, 939
BsiHKAI GWGCWC 1 cut(s) 340
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 1025
BslI CCNNNNNNNGG 3 cut(s) 877, 1048, 1113
BsmAI GTCTC 2 cut(s) 12, 598
BsmFI GGGAC 1 cut(s) 1025
BsnI GGCC 7 cut(s) 167, 291, 491, 580, 632, 638, 939
Bso31I GGTCTC 1 cut(s) 12
Bsp1286I GDGCHC 2 cut(s) 340, 677
Bsp143I GATC 1 cut(s) 276
BspACI CCGC 3 cut(s) 572, 644, 986
BspANI GGCC 7 cut(s) 167, 291, 491, 580, 632, 638, 939
BspCNI CTCAG 2 cut(s) 1008, 1039
BspHI TCATGA 1 cut(s) 612
BspMAI CTGCAG 2 cut(s) 475, 739
BspMI ACCTGC 1 cut(s) 728
BspTI CTTAAG 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 12
BsrDI GCAATG 3 cut(s) 168, 242, 332
BsrI ACTGG 1 cut(s) 912
BssECI CCNNGG 3 cut(s) 633, 639, 681
BssMI GATC 1 cut(s) 276
BssSI CACGAG 1 cut(s) 72
Bst2BI CACGAG 1 cut(s) 72
Bst2UI CCWGG 2 cut(s) 641, 936
Bst4CI ACNGT 2 cut(s) 610, 682
Bst6I CTCTTC 2 cut(s) 51, 660
BstAFI CTTAAG 1 cut(s) 317
BstC8I GCNNGC 1 cut(s) 398
BstDEI CTNAG 5 cut(s) 39, 114, 341, 995, 1047
BstDSI CCRYGG 1 cut(s) 681
BstF5I GGATG 1 cut(s) 1057
BstH2I RGCGCY 1 cut(s) 818
BstHHI GCGC 1 cut(s) 817
BstKTI GATC 1 cut(s) 279
BstMAI GTCTC 2 cut(s) 12, 598
BstMBI GATC 1 cut(s) 276
BstMWI GCNNNNNNNGC 3 cut(s) 68, 279, 288
BstNI CCWGG 2 cut(s) 641, 936
BstSCI CCNGG 3 cut(s) 21, 639, 934
BstSFI CTRYAG 2 cut(s) 471, 735
BstSLI GKGCMC 1 cut(s) 677
BstV1I GCAGC 5 cut(s) 55, 162, 496, 721, 868
BstV2I GAAGAC 1 cut(s) 41
BsuRI GGCC 7 cut(s) 167, 291, 491, 580, 632, 638, 939
BtgI CCRYGG 1 cut(s) 681
BtsCI GGATG 1 cut(s) 1057
BtsI GCAGTG 1 cut(s) 567
BtsIMutI CAGTG 3 cut(s) 567, 606, 905
BveI ACCTGC 1 cut(s) 728
Cac8I GCNNGC 1 cut(s) 398
CciI TCATGA 1 cut(s) 612
CfoI GCGC 1 cut(s) 817
Cfr13I GGNCC 2 cut(s) 371, 637
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
DdeI CTNAG 5 cut(s) 39, 114, 341, 995, 1047
DpnI GATC 1 cut(s) 278
DpnII GATC 1 cut(s) 276
DraI TTTAAA 2 cut(s) 367, 747
EaeI YGGCCR 4 cut(s) 165, 289, 489, 630
Eam1104I CTCTTC 2 cut(s) 51, 660
EarI CTCTTC 2 cut(s) 51, 660
Eco31I GGTCTC 1 cut(s) 12
Eco47I GGWCC 1 cut(s) 371
Eco47III AGCGCT 1 cut(s) 816
Eco57I CTGAAG 3 cut(s) 684, 878, 1089
EcoRI GAATTC 2 cut(s) 307, 794
EcoRII CCWGG 2 cut(s) 639, 934
FalI AAGNNNNNCTT 2 cut(s) 827, 859
FaqI GGGAC 1 cut(s) 1025
Fnu4HI GCNGC 5 cut(s) 69, 151, 510, 735, 882
FokI GGATG 1 cut(s) 1064
Fsp4HI GCNGC 5 cut(s) 69, 151, 510, 735, 882
FspBI CTAG 3 cut(s) 227, 333, 479
GlaI GCGC 1 cut(s) 816
GluI GCNGC 5 cut(s) 69, 151, 510, 735, 882
GsuI CTGGAG 2 cut(s) 207, 269
HaeII RGCGCY 1 cut(s) 818
HaeIII GGCC 7 cut(s) 167, 291, 491, 580, 632, 638, 939
HapII CCGG 1 cut(s) 23
HhaI GCGC 1 cut(s) 817
Hin6I GCGC 1 cut(s) 815
HinP1I GCGC 1 cut(s) 815
HincII GTYRAC 1 cut(s) 195
HindII GTYRAC 1 cut(s) 195
HinfI GANTC 3 cut(s) 77, 134, 944
HpaI GTTAAC 1 cut(s) 195
HpaII CCGG 1 cut(s) 23
Hpy166II GTNNAC 2 cut(s) 195, 606
Hpy188I TCNGA 4 cut(s) 313, 503, 890, 1048
Hpy188III TCNNGA 7 cut(s) 17, 74, 186, 446, 613, 997, 1135
Hpy8I GTNNAC 2 cut(s) 195, 606
HpyAV CCTTC 4 cut(s) 452, 664, 1064, 1141
HpyCH4III ACNGT 2 cut(s) 610, 682
HpyF10VI GCNNNNNNNGC 3 cut(s) 68, 279, 288
HpyF3I CTNAG 5 cut(s) 39, 114, 341, 995, 1047
HspAI GCGC 1 cut(s) 815
KspAI GTTAAC 1 cut(s) 195
Kzo9I GATC 1 cut(s) 276
LmnI GCTCC 1 cut(s) 158
Lsp1109I GCAGC 5 cut(s) 55, 162, 496, 721, 868
LweI GCATC 2 cut(s) 312, 712
MaeI CTAG 3 cut(s) 227, 333, 479
MaeIII GTNAC 2 cut(s) 646, 1073
MalI GATC 1 cut(s) 278
MboI GATC 1 cut(s) 276
MhlI GDGCHC 2 cut(s) 340, 677
MluCI AATT 8 cut(s) 104, 253, 307, 794, 872, 972, 989, 1080
MlyI GAGTC 2 cut(s) 143, 953
MroXI GAANNNNTTC 1 cut(s) 465
MseI TTAA 8 cut(s) 107, 141, 194, 318, 360, 366, 746, 827
MslI CAYNNNNRTG 4 cut(s) 611, 680, 708, 1008
MspCI CTTAAG 1 cut(s) 317
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 3 cut(s) 23, 641, 936
MvaI CCWGG 2 cut(s) 641, 936
MwoI GCNNNNNNNGC 3 cut(s) 68, 279, 288
NciI CCSGG 1 cut(s) 23
NdeII GATC 1 cut(s) 276
NmeAIII GCCGAG 1 cut(s) 658
OliI CACNNNNGTG 1 cut(s) 680
PagI TCATGA 1 cut(s) 612
PaqCI CACCTGC 1 cut(s) 728
PdmI GAANNNNTTC 1 cut(s) 465
PfeI GAWTC 1 cut(s) 77
PflMI CCANNNNNTGG 1 cut(s) 1113
PkrI GCNGC 5 cut(s) 70, 152, 511, 736, 883
PleI GAGTC 2 cut(s) 142, 952
PpsI GAGTC 2 cut(s) 142, 952
PshBI ATTAAT 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 639, 934
PspGI CCWGG 2 cut(s) 639, 934
PspPI GGNCC 2 cut(s) 371, 637
PstI CTGCAG 2 cut(s) 475, 739
RseI CAYNNNNRTG 4 cut(s) 611, 680, 708, 1008
SaqAI TTAA 8 cut(s) 107, 141, 194, 318, 360, 366, 746, 827
SatI GCNGC 5 cut(s) 69, 151, 510, 735, 882
Sau3AI GATC 1 cut(s) 276
Sau96I GGNCC 2 cut(s) 371, 637
SchI GAGTC 2 cut(s) 143, 953
ScrFI CCNGG 3 cut(s) 23, 641, 936
SduI GDGCHC 2 cut(s) 340, 677
SfaNI GCATC 2 cut(s) 312, 712
SfcI CTRYAG 2 cut(s) 471, 735
SinI GGWCC 1 cut(s) 371
SmiMI CAYNNNNRTG 4 cut(s) 611, 680, 708, 1008
SmlI CTYRAG 2 cut(s) 89, 317
SmoI CTYRAG 2 cut(s) 89, 317
SpeI ACTAGT 1 cut(s) 332
Sse9I AATT 8 cut(s) 104, 253, 307, 794, 872, 972, 989, 1080
SsiI CCGC 3 cut(s) 572, 644, 986
SspI AATATT 2 cut(s) 380, 560
SspMI CTAG 3 cut(s) 227, 333, 479
StyD4I CCNGG 3 cut(s) 21, 639, 934
TaaI ACNGT 2 cut(s) 610, 682
TaqI TCGA 3 cut(s) 16, 301, 898
TasI AATT 8 cut(s) 104, 253, 307, 794, 872, 972, 989, 1080
TfiI GAWTC 1 cut(s) 77
Tru1I TTAA 8 cut(s) 107, 141, 194, 318, 360, 366, 746, 827
Tru9I TTAA 8 cut(s) 107, 141, 194, 318, 360, 366, 746, 827
TscAI CASTG 3 cut(s) 574, 613, 912
TseI GCWGC 5 cut(s) 68, 150, 509, 734, 881
TspDTI ATGAA 6 cut(s) 336, 512, 744, 787, 1020, 1026
TspRI CASTG 3 cut(s) 574, 613, 912
Van91I CCANNNNNTGG 1 cut(s) 1113
Vha464I CTTAAG 1 cut(s) 317
VpaK11BI GGWCC 1 cut(s) 371
VspI ATTAAT 1 cut(s) 141
XapI RAATTY 3 cut(s) 253, 307, 794
XmnI GAANNNNTTC 1 cut(s) 465
XspI CTAG 3 cut(s) 227, 333, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.