FvH4_4g29030

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0011268
Physical Location & Seq
Reverse (-)
29385741 .. 29388492
2752 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g29030.t1

Sequence Viewer

Length: 1146 bp
ATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTTGCATTTTTTCCACCAGATCCTCCATCATATAACATATTCGTCGAAGAACCTACTGGGAAGATGAGGATCTCTGATGTTCATCCCAGAGAAGATGTGGACGTTATGACGATCAACACCAAGAAAGGGAATGAGATTGTTGCCTTGTTTATCAAAAATCCATCTGCTTCACTCACTATGCTCTACTCTCATGGAAATGCTGCTGATATTGGCCAGATGTACCACGTTTTCAGTGAGCTTAGTGTTTATCTGGGTGTTAATCTAATGGGGTATGACTATTCTGGTTACGGACAATCTTCTGGAAAGCCAAGTGAGCAGGACACATATGCAGACATAGAGGCTGCATATAAATGCCTTGAAGAAACCTATGGTGTAAAGGAGGAAGATGTCATATTGTACGGACAGTCAGTTGGAAGTGGACCTACTTTGGATTTGGCTGCTCGTTTGCCTAATCTGAGAGCTGTGGTTCTTCACAGTCCAATACTTTCTGGTCTTCGAGTAATGTACCCTGTTAAGAAAACATTCTGGTTTGACATTTACAAGAATATTGATAAAATGCCACTCGTCAATAGCCCGGTTTTGGTGATTCATGGAACTGAAGATGATGTGGTGGATATCTCCCATGGTAAGCAGCTGTGGGAGCTTAGCAAAGACAAGTATGAACCTTTGTGGCTTAAAGGAGGTAACCATAGTAACCTGGAACTCTATCCAGAGTACTTGAGGCATCTCAGGAAGTTCATATCTGCCGTTGAGAAGCTACCACGACACCGGAATCTAACTGAGCTGAGTCCTCTCAAGACAATAGATGACAGCAAAAAGGCAAGATCAAGCAGTGGAAAGAGGGACAAGGCTAGGTGGAGCACCGGGCAGCTGAGAGAAAAATCAAGAAGGAGCACAGAGAATAGAGAGAAACCGTGGTCAAGAAGCAGTACTTATAGTAGAGAAAAATCGGGGAGAAAACAAAGTGTGGATCGATCTGGGAAGGCAAGGAATAGCACAGACTCGGATAGAGCAAGAAACAGCTTCGACCGCTTGGGAGATATGGTGAGATCAGTTGGATTGTGCAATGTTGATTGTATGAAGCCAGCAGCTTTCGAAAATTTCGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

382

Amino Acids

42.9

Weight (kDa)

8.82

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 70 - 175 3.3e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 171 7.3e-06 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 1063
AclWI GGATC 3 cut(s) 47, 110, 1011
AcoI YGGCCR 1 cut(s) 244
AcsI RAATTY 1 cut(s) 1132
AcuI CTGAAG 1 cut(s) 651
AfaI GTAC 5 cut(s) 254, 431, 539, 749, 964
AfiI CCNNNNNNNGG 2 cut(s) 159, 613
AgsI TTSAA 1 cut(s) 392
AjnI CCWGG 1 cut(s) 729
AluBI AGCT 9 cut(s) 271, 494, 667, 676, 790, 817, 904, 1056, 1124
AluI AGCT 9 cut(s) 271, 494, 667, 676, 790, 817, 904, 1056, 1124
Alw21I GWGCWC 2 cut(s) 896, 929
AlwI GGATC 3 cut(s) 47, 110, 1011
AoxI GGCC 1 cut(s) 244
ApeKI GCWGC 7 cut(s) 26, 233, 374, 470, 664, 901, 1121
ApoI RAATTY 1 cut(s) 1132
Asp700I GAANNNNTTC 1 cut(s) 554
AspS9I GGNCC 1 cut(s) 452
AsuC2I CCSGG 2 cut(s) 608, 898
AsuHPI GGTGA 2 cut(s) 628, 1090
AsuII TTCGAA 1 cut(s) 1128
AvaII GGWCC 1 cut(s) 452
BalI TGGCCA 1 cut(s) 246
BarI GAAGNNNNNNTAC 2 cut(s) 439, 471
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 2 cut(s) 896, 929
BbvI GCAGC 7 cut(s) 13, 220, 361, 457, 676, 913, 1133
BccI CCATC 2 cut(s) 67, 202
BceAI ACGGC 1 cut(s) 764
BciT130I CCWGG 1 cut(s) 731
BcnI CCSGG 2 cut(s) 608, 898
BfaI CTAG 1 cut(s) 885
BisI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
BlpI GCTNAGC 1 cut(s) 677
BlsI GCNGC 7 cut(s) 28, 235, 376, 472, 666, 903, 1123
BmcAI AGTACT 2 cut(s) 749, 964
Bme1390I CCNGG 3 cut(s) 608, 731, 898
Bme18I GGWCC 1 cut(s) 452
BmgT120I GGNCC 1 cut(s) 452
BmrFI CCNGG 3 cut(s) 608, 731, 898
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 2 cut(s) 766, 1129
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 518
Bpu1102I GCTNAGC 1 cut(s) 677
Bpu14I TTCGAA 1 cut(s) 1128
BpuEI CTTGAG 2 cut(s) 772, 812
BpuMI CCSGG 2 cut(s) 608, 898
Bsa29I ATCGAT 1 cut(s) 1006
BsaBI GATNNNNATC 2 cut(s) 101, 114
BsaJI CCNNGG 2 cut(s) 655, 947
BsaWI WCCGGW 1 cut(s) 801
BsaXI ACNNNNNCTCC 2 cut(s) 1062, 1092
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 613
Bse1I ACTGG 1 cut(s) 94
Bse3DI GCAATG 1 cut(s) 1105
Bse8I GATNNNNATC 2 cut(s) 101, 114
BseBI CCWGG 1 cut(s) 731
BseCI ATCGAT 1 cut(s) 1006
BseDI CCNNGG 2 cut(s) 655, 947
BseGI GGATG 1 cut(s) 115
BseJI GATNNNNATC 2 cut(s) 101, 114
BseLI CCNNNNNNNGG 2 cut(s) 159, 613
BseMI GCAATG 1 cut(s) 1105
BseMII CTCAG 5 cut(s) 479, 775, 804, 809, 896
BseNI ACTGG 1 cut(s) 94
BseXI GCAGC 7 cut(s) 13, 220, 361, 457, 676, 913, 1133
Bsh1285I CGRYCG 1 cut(s) 1063
BshFI GGCC 1 cut(s) 246
BshVI ATCGAT 1 cut(s) 1006
BsiEI CGRYCG 1 cut(s) 1063
BsiHKAI GWGCWC 2 cut(s) 896, 929
BsiSI CCGG 3 cut(s) 608, 802, 897
BslFI GGGAC 1 cut(s) 890
BslI CCNNNNNNNGG 2 cut(s) 159, 613
BsmFI GGGAC 1 cut(s) 890
BsnI GGCC 1 cut(s) 246
Bsp119I TTCGAA 1 cut(s) 1128
Bsp1286I GDGCHC 2 cut(s) 896, 929
Bsp143I GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
Bsp1720I GCTNAGC 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 1 cut(s) 1063
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 5 cut(s) 480, 774, 805, 810, 897
BspDI ATCGAT 1 cut(s) 1006
BspPI GGATC 3 cut(s) 47, 110, 1011
BspT104I TTCGAA 1 cut(s) 1128
BsrDI GCAATG 1 cut(s) 1105
BsrI ACTGG 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 655, 947
BssMI GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
BssT1I CCWWGG 1 cut(s) 655
Bst2UI CCWGG 1 cut(s) 731
Bst4CI ACNGT 3 cut(s) 438, 509, 948
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 1128
BstC8I GCNNGC 1 cut(s) 1119
BstDEI CTNAG 7 cut(s) 272, 488, 677, 761, 813, 818, 905
BstDSI CCRYGG 2 cut(s) 655, 947
BstEII GGTNACC 1 cut(s) 716
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 7 cut(s) 55, 105, 147, 860, 1006, 1010, 1085
BstMBI GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
BstMCI CGRYCG 1 cut(s) 1063
BstMWI GCNNNNNNNGC 4 cut(s) 35, 346, 673, 1062
BstNI CCWGG 1 cut(s) 731
BstPI GGTNACC 1 cut(s) 716
BstSCI CCNGG 3 cut(s) 606, 729, 896
BstV1I GCAGC 7 cut(s) 13, 220, 361, 457, 676, 913, 1133
BstV2I GAAGAC 1 cut(s) 518
BstX2I RGATCY 2 cut(s) 52, 102
BstYI RGATCY 2 cut(s) 52, 102
Bsu15I ATCGAT 1 cut(s) 1006
BsuRI GGCC 1 cut(s) 246
BsuTUI ATCGAT 1 cut(s) 1006
BtgI CCRYGG 2 cut(s) 655, 947
BtsCI GGATG 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 871
BtsIMutI CAGTG 2 cut(s) 271, 871
Cac8I GCNNGC 1 cut(s) 1119
Cfr13I GGNCC 1 cut(s) 452
ClaI ATCGAT 1 cut(s) 1006
Csp6I GTAC 5 cut(s) 253, 430, 538, 748, 963
CviAII CATG 3 cut(s) 224, 623, 656
CviQI GTAC 5 cut(s) 253, 430, 538, 748, 963
DdeI CTNAG 7 cut(s) 272, 488, 677, 761, 813, 818, 905
DpnI GATC 7 cut(s) 54, 104, 146, 859, 1005, 1009, 1084
DpnII GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
EaeI YGGCCR 1 cut(s) 244
Eco130I CCWWGG 1 cut(s) 655
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 651
Eco91I GGTNACC 1 cut(s) 716
EcoO65I GGTNACC 1 cut(s) 716
EcoRII CCWGG 1 cut(s) 729
EcoRV GATATC 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 3 cut(s) 227, 626, 659
FalI AAGNNNNNCTT 2 cut(s) 949, 981
FaqI GGGAC 1 cut(s) 890
FatI CATG 3 cut(s) 223, 622, 655
FauNDI CATATG 1 cut(s) 358
Fnu4HI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
FokI GGATG 1 cut(s) 102
Fsp4HI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
FspBI CTAG 1 cut(s) 885
GluI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
HaeIII GGCC 1 cut(s) 246
HapII CCGG 3 cut(s) 608, 802, 897
Hin1II CATG 3 cut(s) 227, 626, 659
HinfI GANTC 4 cut(s) 619, 805, 820, 1034
HpaII CCGG 3 cut(s) 608, 802, 897
HphI GGTGA 2 cut(s) 628, 1090
Hpy166II GTNNAC 2 cut(s) 133, 452
Hpy188I TCNGA 3 cut(s) 109, 489, 1039
Hpy188III TCNNGA 6 cut(s) 333, 743, 763, 829, 918, 954
Hpy8I GTNNAC 2 cut(s) 133, 452
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 2 cut(s) 915, 1009
HpyCH4III ACNGT 3 cut(s) 438, 509, 948
HpyCH4IV ACGT 2 cut(s) 135, 258
HpyCH4V TGCA 6 cut(s) 11, 29, 38, 362, 377, 1098
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 346, 673, 1062
HpyF3I CTNAG 7 cut(s) 272, 488, 677, 761, 813, 818, 905
HpySE526I ACGT 2 cut(s) 135, 258
Hsp92II CATG 3 cut(s) 227, 626, 659
Kzo9I GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
LmnI GCTCC 3 cut(s) 673, 891, 924
Lsp1109I GCAGC 7 cut(s) 13, 220, 361, 457, 676, 913, 1133
LweI GCATC 2 cut(s) 766, 1129
MaeI CTAG 1 cut(s) 885
MaeII ACGT 2 cut(s) 135, 258
MaeIII GTNAC 3 cut(s) 317, 716, 725
MalI GATC 7 cut(s) 54, 104, 146, 859, 1005, 1009, 1084
MboI GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
MboII GAAGA 9 cut(s) 92, 106, 137, 321, 404, 428, 494, 518, 644
MflI RGATCY 2 cut(s) 52, 102
MhlI GDGCHC 2 cut(s) 896, 929
MlsI TGGCCA 1 cut(s) 246
MluCI AATT 1 cut(s) 1132
MluNI TGGCCA 1 cut(s) 246
MlyI GAGTC 2 cut(s) 829, 1028
MmeI TCCRAC 2 cut(s) 424, 1069
MnlI CCTC 8 cut(s) 66, 93, 364, 406, 707, 747, 834, 867
Mox20I TGGCCA 1 cut(s) 246
MroXI GAANNNNTTC 1 cut(s) 554
MscI TGGCCA 1 cut(s) 246
MseI TTAA 3 cut(s) 291, 546, 708
MslI CAYNNNNRTG 2 cut(s) 228, 382
Msp20I TGGCCA 1 cut(s) 246
MspA1I CMGCKG 2 cut(s) 667, 904
MspI CCGG 3 cut(s) 608, 802, 897
MspR9I CCNGG 3 cut(s) 608, 731, 898
MvaI CCWGG 1 cut(s) 731
MwoI GCNNNNNNNGC 4 cut(s) 35, 346, 673, 1062
NciI CCSGG 2 cut(s) 608, 898
NcoI CCATGG 1 cut(s) 655
NdeI CATATG 1 cut(s) 358
NdeII GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
NlaIII CATG 3 cut(s) 227, 626, 659
NspV TTCGAA 1 cut(s) 1128
PcsI WCGNNNNNNNCGW 1 cut(s) 1134
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 619, 805
PkrI GCNGC 7 cut(s) 28, 235, 376, 472, 666, 903, 1123
PleI GAGTC 2 cut(s) 828, 1028
PpsI GAGTC 2 cut(s) 828, 1028
Psp6I CCWGG 1 cut(s) 729
PspEI GGTNACC 1 cut(s) 716
PspGI CCWGG 1 cut(s) 729
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 2 cut(s) 52, 102
PvuII CAGCTG 2 cut(s) 667, 904
RsaI GTAC 5 cut(s) 254, 431, 539, 749, 964
RsaNI GTAC 5 cut(s) 253, 430, 538, 748, 963
RseI CAYNNNNRTG 2 cut(s) 228, 382
SaqAI TTAA 3 cut(s) 291, 546, 708
SatI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
Sau3AI GATC 7 cut(s) 52, 102, 144, 857, 1003, 1007, 1082
Sau96I GGNCC 1 cut(s) 452
ScaI AGTACT 2 cut(s) 749, 964
SchI GAGTC 2 cut(s) 829, 1028
ScrFI CCNGG 3 cut(s) 608, 731, 898
SduI GDGCHC 2 cut(s) 896, 929
SfaNI GCATC 2 cut(s) 766, 1129
SfuI TTCGAA 1 cut(s) 1128
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 2 cut(s) 228, 382
SmlI CTYRAG 2 cut(s) 751, 827
SmoI CTYRAG 2 cut(s) 751, 827
Sse9I AATT 1 cut(s) 1132
SsiI CCGC 1 cut(s) 1063
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 885
StyD4I CCNGG 3 cut(s) 606, 729, 896
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 3 cut(s) 438, 509, 948
TaiI ACGT 2 cut(s) 138, 261
TaqI TCGA 6 cut(s) 78, 529, 1006, 1059, 1128, 1137
TasI AATT 1 cut(s) 1132
TatI WGTACW 2 cut(s) 747, 962
TfiI GAWTC 2 cut(s) 619, 805
Tru1I TTAA 3 cut(s) 291, 546, 708
Tru9I TTAA 3 cut(s) 291, 546, 708
TscAI CASTG 2 cut(s) 271, 871
TseI GCWGC 7 cut(s) 26, 233, 374, 470, 664, 901, 1121
TspDTI ATGAA 5 cut(s) 104, 611, 708, 760, 1127
TspGWI ACGGA 2 cut(s) 336, 447
TspRI CASTG 2 cut(s) 271, 871
VpaK11BI GGWCC 1 cut(s) 452
XapI RAATTY 1 cut(s) 1132
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 1 cut(s) 885
ZrmI AGTACT 2 cut(s) 749, 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.