Rw4G030930

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0011268
Physical Location & Seq
Reverse (-)
57725564 .. 57727675
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G030930.1

Sequence Viewer

Length: 1086 bp
ATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTTGCATTTTTTCCCCCAGATCCTCCTTCCTATAACATATTCGTTGATGAACCGACTGGGAAAATGAGGATCTCTGATGTTCATCCCAGACAAGATGTGGACGTGGTGAAGATCAACACCAAGAAAGGGAACGAGATTGTTGCCTTGTTTATCAAAAACCCATCTGCTTCACTCACAGTGCTTTACTCTCATGGAAATGCTGCTGATATTGGTCAGATGTATGACTATTCTGGTTATGGACAATCTACTGGAAAGCCAAGTGAGCAGGACACATATGCAGACATAGAGGCTGCATATAAATGCCTTGAAGACACCTATGGTGTAAAGGAGGAAGATATTATATTGTACGGACAGTCAGTTGGAAGTGGACCGACTTTGGATTTGGCTAGTCGTTTGCCTCACGTGAGAGCTGTGATTCTTCACAGTCCAATACTTTCTGGCCTTCGAGTAATGTACCCTGTTAAGAAAACATTCTGGTTTGACATTTACAAGAATATTGATAAAATGCCACTCGTCAATAGCCCGGTTTTGGTGATTCATGGAACTGAAGATGATGTGGTGGATATTTCCCATGGTAAGCAGCTGTGGGAGCTTAGCAAAGACAAGTATGAACCTTTGTGGCTTAAAGGTGGTAACCACTGTAATCTGGAACTCTATCCAGAGTACTTGAGGCATCTCAGGAAGTTCATATCTGCCATTGAGAAGTTACCACGACACCGGAATCTAACTGAGCTGAGTCCTCTCAAGACCGTAGACGACAATGACAAAGCAAGACCAAGCAGTGGGAAGAGGGGCAAGGCTAGGTGGAGCACCGGGCAGCTGAGAGAAAAATCAAGGAGGAGCACAGAGAATGGAGAGAAATCGTCATCAAGAAGCAGTACTTATAGTAGAGAGAAATCATCGAGAAAGCTAAGTGTGGATCGATCTGGGAAGGCAAGGAATAGCACAGACTCAGATAGAGCAAGAAACAGCTTCGACCGCTTGGGAGATATGGTGAGATCAGTTGGATTGTGCAATGTTGATTGTATGAAGCCGGCAGCTTTCGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

361

Amino Acids

40.4

Weight (kDa)

9.0

Isoelectric Point (pI)

54.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 84 - 158 8.6e-06 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 815
AccI GTMKAC 1 cut(s) 786
AciI CCGC 1 cut(s) 1012
AclWI GGATC 3 cut(s) 47, 110, 960
AcuI CTGAAG 1 cut(s) 600
AcvI CACGTG 1 cut(s) 436
AfaI GTAC 4 cut(s) 380, 488, 698, 913
AfiI CCNNNNNNNGG 3 cut(s) 159, 562, 815
AgsI TTSAA 1 cut(s) 341
AjiI CACGTC 1 cut(s) 136
AluBI AGCT 8 cut(s) 443, 616, 625, 766, 853, 943, 1005, 1073
AluI AGCT 8 cut(s) 443, 616, 625, 766, 853, 943, 1005, 1073
Alw21I GWGCWC 2 cut(s) 845, 878
AlwI GGATC 3 cut(s) 47, 110, 960
AoxI GGCC 1 cut(s) 472
ApeKI GCWGC 6 cut(s) 26, 233, 323, 613, 850, 1070
ArsI GACNNNNNNTTYG 2 cut(s) 397, 429
Asp700I GAANNNNTTC 1 cut(s) 503
AspS9I GGNCC 1 cut(s) 401
AsuC2I CCSGG 2 cut(s) 557, 847
AsuHPI GGTGA 3 cut(s) 151, 577, 1039
AvaII GGWCC 1 cut(s) 401
BbrPI CACGTG 1 cut(s) 436
BbsI GAAGAC 1 cut(s) 348
Bbv12I GWGCWC 2 cut(s) 845, 878
BbvI GCAGC 6 cut(s) 13, 220, 310, 625, 862, 1082
BccI CCATC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BcnI CCSGG 2 cut(s) 557, 847
BfaI CTAG 2 cut(s) 420, 834
BisI GCNGC 6 cut(s) 27, 234, 324, 614, 851, 1071
BlpI GCTNAGC 1 cut(s) 626
BlsI GCNGC 6 cut(s) 28, 235, 325, 615, 852, 1072
BmcAI AGTACT 2 cut(s) 698, 913
Bme1390I CCNGG 2 cut(s) 557, 847
Bme18I GGWCC 1 cut(s) 401
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 401
BmrFI CCNGG 2 cut(s) 557, 847
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 715
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 348
Bpu1102I GCTNAGC 1 cut(s) 626
BpuEI CTTGAG 2 cut(s) 721, 761
BpuMI CCSGG 2 cut(s) 557, 847
Bsa29I ATCGAT 1 cut(s) 955
BsaAI YACGTR 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 604
BsaWI WCCGGW 1 cut(s) 750
BsaXI ACNNNNNCTCC 2 cut(s) 1011, 1041
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 562, 815
Bse118I RCCGGY 1 cut(s) 1066
Bse1I ACTGG 2 cut(s) 94, 286
Bse3DI GCAATG 1 cut(s) 1054
Bse8I GATNNNNATC 1 cut(s) 114
BseCI ATCGAT 1 cut(s) 955
BseDI CCNNGG 1 cut(s) 604
BseGI GGATG 1 cut(s) 115
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 3 cut(s) 159, 562, 815
BseMI GCAATG 1 cut(s) 1054
BseMII CTCAG 5 cut(s) 724, 753, 758, 845, 999
BseNI ACTGG 2 cut(s) 94, 286
BseRI GAGGAG 1 cut(s) 886
BseXI GCAGC 6 cut(s) 13, 220, 310, 625, 862, 1082
Bsh1285I CGRYCG 1 cut(s) 1012
BshFI GGCC 1 cut(s) 474
BshVI ATCGAT 1 cut(s) 955
BsiEI CGRYCG 1 cut(s) 1012
BsiHKAI GWGCWC 2 cut(s) 845, 878
BsiSI CCGG 4 cut(s) 557, 751, 846, 1067
BslI CCNNNNNNNGG 3 cut(s) 159, 562, 815
BsnI GGCC 1 cut(s) 474
Bsp1286I GDGCHC 2 cut(s) 845, 878
Bsp143I GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
Bsp1720I GCTNAGC 1 cut(s) 626
Bsp19I CCATGG 1 cut(s) 604
BspACI CCGC 1 cut(s) 1012
BspANI GGCC 1 cut(s) 474
BspCNI CTCAG 5 cut(s) 723, 754, 759, 846, 998
BspDI ATCGAT 1 cut(s) 955
BspPI GGATC 3 cut(s) 47, 110, 960
BsrDI GCAATG 1 cut(s) 1054
BsrFI RCCGGY 1 cut(s) 1066
BsrI ACTGG 2 cut(s) 94, 286
BssAI RCCGGY 1 cut(s) 1066
BssECI CCNNGG 1 cut(s) 604
BssMI GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
BssT1I CCWWGG 1 cut(s) 604
Bst4CI ACNGT 5 cut(s) 211, 387, 458, 674, 784
Bst6I CTCTTC 1 cut(s) 815
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 436
BstC8I GCNNGC 1 cut(s) 1068
BstDEI CTNAG 7 cut(s) 626, 710, 762, 767, 854, 944, 985
BstDSI CCRYGG 1 cut(s) 604
BstEII GGTNACC 1 cut(s) 665
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 6 cut(s) 55, 105, 147, 955, 959, 1034
BstMBI GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
BstMCI CGRYCG 1 cut(s) 1012
BstMWI GCNNNNNNNGC 5 cut(s) 35, 295, 622, 1011, 1079
BstPI GGTNACC 1 cut(s) 665
BstSCI CCNGG 2 cut(s) 555, 845
BstV1I GCAGC 6 cut(s) 13, 220, 310, 625, 862, 1082
BstV2I GAAGAC 1 cut(s) 348
BstX2I RGATCY 2 cut(s) 52, 102
BstYI RGATCY 2 cut(s) 52, 102
Bsu15I ATCGAT 1 cut(s) 955
BsuRI GGCC 1 cut(s) 474
BsuTUI ATCGAT 1 cut(s) 955
BtgI CCRYGG 1 cut(s) 604
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 3 cut(s) 216, 670, 820
Cac8I GCNNGC 1 cut(s) 1068
Cfr10I RCCGGY 1 cut(s) 1066
Cfr13I GGNCC 1 cut(s) 401
ClaI ATCGAT 1 cut(s) 955
Csp6I GTAC 4 cut(s) 379, 487, 697, 912
CviAII CATG 3 cut(s) 224, 572, 605
CviQI GTAC 4 cut(s) 379, 487, 697, 912
DdeI CTNAG 7 cut(s) 626, 710, 762, 767, 854, 944, 985
DpnI GATC 6 cut(s) 54, 104, 146, 954, 958, 1033
DpnII GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
Eam1104I CTCTTC 1 cut(s) 815
EarI CTCTTC 1 cut(s) 815
Eco130I CCWWGG 1 cut(s) 604
Eco47I GGWCC 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 600
Eco72I CACGTG 1 cut(s) 436
Eco91I GGTNACC 1 cut(s) 665
EcoO65I GGTNACC 1 cut(s) 665
EcoT14I CCWWGG 1 cut(s) 604
ErhI CCWWGG 1 cut(s) 604
FaeI CATG 3 cut(s) 227, 575, 608
FalI AAGNNNNNCTT 2 cut(s) 898, 930
FatI CATG 3 cut(s) 223, 571, 604
FauNDI CATATG 1 cut(s) 307
FblI GTMKAC 1 cut(s) 786
Fnu4HI GCNGC 6 cut(s) 27, 234, 324, 614, 851, 1071
FokI GGATG 1 cut(s) 102
Fsp4HI GCNGC 6 cut(s) 27, 234, 324, 614, 851, 1071
FspBI CTAG 2 cut(s) 420, 834
GluI GCNGC 6 cut(s) 27, 234, 324, 614, 851, 1071
HaeIII GGCC 1 cut(s) 474
HapII CCGG 4 cut(s) 557, 751, 846, 1067
Hin1II CATG 3 cut(s) 227, 575, 608
HinfI GANTC 5 cut(s) 448, 568, 754, 769, 983
HpaII CCGG 4 cut(s) 557, 751, 846, 1067
HphI GGTGA 3 cut(s) 151, 577, 1039
Hpy166II GTNNAC 3 cut(s) 133, 401, 787
Hpy188I TCNGA 3 cut(s) 109, 249, 988
Hpy188III TCNNGA 6 cut(s) 680, 692, 712, 778, 903, 936
Hpy8I GTNNAC 3 cut(s) 133, 401, 787
HpyAV CCTTC 3 cut(s) 69, 485, 958
HpyCH4III ACNGT 5 cut(s) 211, 387, 458, 674, 784
HpyCH4IV ACGT 2 cut(s) 135, 435
HpyCH4V TGCA 6 cut(s) 11, 29, 38, 311, 326, 1047
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 295, 622, 1011, 1079
HpyF3I CTNAG 7 cut(s) 626, 710, 762, 767, 854, 944, 985
HpySE526I ACGT 2 cut(s) 135, 435
Hsp92II CATG 3 cut(s) 227, 575, 608
KroI GCCGGC 1 cut(s) 1066
KroNI GCCGGC 1 cut(s) 1068
Kzo9I GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
LmnI GCTCC 3 cut(s) 622, 840, 873
Lsp1109I GCAGC 6 cut(s) 13, 220, 310, 625, 862, 1082
LweI GCATC 1 cut(s) 715
MaeI CTAG 2 cut(s) 420, 834
MaeII ACGT 2 cut(s) 135, 435
MaeIII GTNAC 2 cut(s) 665, 738
MalI GATC 6 cut(s) 54, 104, 146, 954, 958, 1033
MboI GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
MboII GAAGA 6 cut(s) 154, 353, 377, 443, 593, 832
MflI RGATCY 2 cut(s) 52, 102
MhlI GDGCHC 2 cut(s) 845, 878
MlyI GAGTC 2 cut(s) 778, 977
MmeI TCCRAC 2 cut(s) 373, 1018
MroNI GCCGGC 1 cut(s) 1066
MroXI GAANNNNTTC 1 cut(s) 503
MseI TTAA 2 cut(s) 495, 657
MslI CAYNNNNRTG 2 cut(s) 228, 331
MspA1I CMGCKG 2 cut(s) 616, 853
MspI CCGG 4 cut(s) 557, 751, 846, 1067
MspR9I CCNGG 2 cut(s) 557, 847
MwoI GCNNNNNNNGC 5 cut(s) 35, 295, 622, 1011, 1079
NaeI GCCGGC 1 cut(s) 1068
NciI CCSGG 2 cut(s) 557, 847
NcoI CCATGG 1 cut(s) 604
NdeI CATATG 1 cut(s) 307
NdeII GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
NgoMIV GCCGGC 1 cut(s) 1066
NlaIII CATG 3 cut(s) 227, 575, 608
PdiI GCCGGC 1 cut(s) 1068
PdmI GAANNNNTTC 1 cut(s) 503
PfeI GAWTC 3 cut(s) 448, 568, 754
PflMI CCANNNNNTGG 1 cut(s) 815
PkrI GCNGC 6 cut(s) 28, 235, 325, 615, 852, 1072
PleI GAGTC 2 cut(s) 777, 977
PmaCI CACGTG 1 cut(s) 436
PmlI CACGTG 1 cut(s) 436
PpsI GAGTC 2 cut(s) 777, 977
Ppu21I YACGTR 1 cut(s) 436
PspCI CACGTG 1 cut(s) 436
PspEI GGTNACC 1 cut(s) 665
PspPI GGNCC 1 cut(s) 401
PsuI RGATCY 2 cut(s) 52, 102
PvuII CAGCTG 2 cut(s) 616, 853
RsaI GTAC 4 cut(s) 380, 488, 698, 913
RsaNI GTAC 4 cut(s) 379, 487, 697, 912
RseI CAYNNNNRTG 2 cut(s) 228, 331
SaqAI TTAA 2 cut(s) 495, 657
SatI GCNGC 6 cut(s) 27, 234, 324, 614, 851, 1071
Sau3AI GATC 6 cut(s) 52, 102, 144, 952, 956, 1031
Sau96I GGNCC 1 cut(s) 401
ScaI AGTACT 2 cut(s) 698, 913
SchI GAGTC 2 cut(s) 778, 977
ScrFI CCNGG 2 cut(s) 557, 847
SduI GDGCHC 2 cut(s) 845, 878
SfaNI GCATC 1 cut(s) 715
SinI GGWCC 1 cut(s) 401
SmiMI CAYNNNNRTG 2 cut(s) 228, 331
SmlI CTYRAG 2 cut(s) 700, 776
SmoI CTYRAG 2 cut(s) 700, 776
SsiI CCGC 1 cut(s) 1012
SspI AATATT 1 cut(s) 529
SspMI CTAG 2 cut(s) 420, 834
StyD4I CCNGG 2 cut(s) 555, 845
StyI CCWWGG 1 cut(s) 604
TaaI ACNGT 5 cut(s) 211, 387, 458, 674, 784
TaiI ACGT 2 cut(s) 138, 438
TaqI TCGA 5 cut(s) 478, 935, 955, 1008, 1077
TaqII GACCGA 1 cut(s) 418
TatI WGTACW 2 cut(s) 696, 911
TfiI GAWTC 3 cut(s) 448, 568, 754
Tru1I TTAA 2 cut(s) 495, 657
Tru9I TTAA 2 cut(s) 495, 657
TscAI CASTG 3 cut(s) 216, 677, 820
TseI GCWGC 6 cut(s) 26, 233, 323, 613, 850, 1070
TspDTI ATGAA 6 cut(s) 96, 104, 560, 657, 709, 1076
TspGWI ACGGA 1 cut(s) 396
TspRI CASTG 3 cut(s) 216, 677, 820
Van91I CCANNNNNTGG 1 cut(s) 815
VpaK11BI GGWCC 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 786
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 2 cut(s) 420, 834
ZrmI AGTACT 2 cut(s) 698, 913
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.