MD16G1054200.v1.1

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0011268
Physical Location & Seq
Reverse (-)
3831255 .. 3833825
2571 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1054200.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTCGCATTTTTCCCACCGGACCCTCCGTCATATGACACGTACATCGACGAACCAACCGGGAAAATGAGGATCTCCGATGTTCTTCCTCGAGACGATGTTGACGTGGTGAAGCTGAGCACAAAAAAAGGGAATGAGATTGTGGCTATGTATGTCAAAAATCCATGTGCTTCACTCACTCTGCTTTACTCCCATGGAAATGCTGCTGATATTGGTCAGATGTACCACATTTTCACTGAGCTTAGTCTTCATCTGGGTGTCAATCTCATGGGCTACGATTATTCTGGCTATGGACAATCTTCTGGAAAGCCAAGTGAGCAGGACACATATGCAGACATAGAGGCAGCATACAAATGCCTTGAAGAGAAGTACGGCATAAAGGAGGAAGATGTTATTTTGTACGGACAGTCAGTTGGAAGCGGACCTGCATTGGATTTGGCCATTCGTTTGCCTAACTTGAGGGGTGTTGTTCTTCACAGTCCGATACTGTCCGGCCTTCGAGTGATGTACGCTGTCAAGAAAACATTCTGGTTCGACATTTATAAGAATATCGATAAGATCCCACAAGTCAGTTGCCCAGTTTTGGTGATTCATGGAACTGAAGATGAAGTTGTGGATTTCTCGCATGGAAAACAGCTGTGGGAGCTTTGCAAAGAGAAATATGAACCCTTGTGGCTCAAAGGAGGCAACCATTGTAACTTGGAACTCTATCCAGAGTACTTGAGGCATCTCAGGAAGTTCATATCTGCCATAGAGAAACTACCCCGTCTTCGAAATTCAACCGAACAGAGTGCAGATCAATCGGAGTCTCCTCTGAAGACCGAAGACAACAAGGACAAGGCAAGATCAAGCACTGACTTGAGAGACAAGTCTAGGTCCAGCACGGGGCAGCGAGACAAGTCCAGGCTCAGCACAGACAGTAAAGAGAGATCAAGAACAAGTACTGAGAAGAGAGAGAAGTCAAGAAGGAGCACAGATCGGTCTGGAAAGGCAAGGAGCAGCATAGATCAGTCGGAGAGAGCTAGAAATAGCTTCGACCGATTGGGAGATATGGTGAGGTCAGTTGGATTATGTAACGTAGATTGTCTGAAGCAGGCCGCTTTAGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

381

Amino Acids

42.68

Weight (kDa)

7.58

Isoelectric Point (pI)

60.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 70 - 174 1.7e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 171 5.9e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
Acc36I ACCTGC 1 cut(s) 463
AciI CCGC 2 cut(s) 450, 1128
AclWI GGATC 2 cut(s) 110, 583
AcoI YGGCCR 1 cut(s) 468
AcsI RAATTY 1 cut(s) 805
AcuI CTGAAG 3 cut(s) 651, 866, 1139
AfaI GTAC 7 cut(s) 74, 254, 401, 431, 539, 749, 973
AfiI CCNNNNNNNGG 2 cut(s) 613, 915
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 2 cut(s) 392, 810
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 1 cut(s) 932
AluBI AGCT 6 cut(s) 145, 271, 667, 676, 1052, 1062
AluI AGCT 6 cut(s) 145, 271, 667, 676, 1052, 1062
Alw21I GWGCWC 2 cut(s) 152, 1004
Alw26I GTCTC 4 cut(s) 117, 843, 888, 918
AlwI GGATC 2 cut(s) 110, 583
Ama87I CYCGRG 1 cut(s) 120
AoxI GGCC 3 cut(s) 468, 523, 1125
ApeKI GCWGC 5 cut(s) 26, 233, 374, 919, 1029
ApoI RAATTY 1 cut(s) 805
Asp700I GAANNNNTTC 1 cut(s) 554
AspS9I GGNCC 3 cut(s) 52, 452, 906
AsuC2I CCSGG 1 cut(s) 91
AsuHPI GGTGA 3 cut(s) 151, 628, 1096
AsuII TTCGAA 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 3 cut(s) 52, 452, 906
BalI TGGCCA 1 cut(s) 470
BbsI GAAGAC 4 cut(s) 269, 791, 854, 861
Bbv12I GWGCWC 2 cut(s) 152, 1004
BbvI GCAGC 5 cut(s) 13, 220, 386, 931, 1041
BceAI ACGGC 1 cut(s) 418
BcgI CGANNNNNNTGC 4 cut(s) 803, 813, 837, 847
BciT130I CCWGG 1 cut(s) 934
BcnI CCSGG 1 cut(s) 91
BcoDI GTCTC 4 cut(s) 117, 843, 888, 918
BfaI CTAG 2 cut(s) 903, 1053
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 6 cut(s) 27, 234, 375, 920, 1030, 1128
BlpI GCTNAGC 2 cut(s) 146, 938
BlsI GCNGC 6 cut(s) 28, 235, 376, 921, 1031, 1129
BmcAI AGTACT 2 cut(s) 749, 973
Bme1390I CCNGG 2 cut(s) 91, 934
Bme18I GGWCC 3 cut(s) 52, 452, 906
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 3 cut(s) 52, 452, 906
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 2 cut(s) 91, 934
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 4 cut(s) 269, 791, 854, 861
Bpu1102I GCTNAGC 2 cut(s) 146, 938
Bpu14I TTCGAA 1 cut(s) 802
BpuEI CTTGAG 3 cut(s) 508, 772, 910
BpuMI CCSGG 1 cut(s) 91
Bsa29I ATCGAT 1 cut(s) 582
BsaAI YACGTR 1 cut(s) 72
BsaJI CCNNGG 1 cut(s) 223
BsaWI WCCGGW 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 1068, 1098
Bsc4I CCNNNNNNNGG 2 cut(s) 613, 915
Bse1I ACTGG 1 cut(s) 608
BseBI CCWGG 1 cut(s) 934
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 1 cut(s) 223
BseLI CCNNNNNNNGG 2 cut(s) 613, 915
BseMII CTCAG 5 cut(s) 137, 258, 775, 952, 966
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 1 cut(s) 831
BseXI GCAGC 5 cut(s) 13, 220, 386, 931, 1041
BsgI GTGCAG 1 cut(s) 843
Bsh1285I CGRYCG 1 cut(s) 1069
BshFI GGCC 3 cut(s) 470, 525, 1127
BshVI ATCGAT 1 cut(s) 582
BsiEI CGRYCG 1 cut(s) 1069
BsiHKAI GWGCWC 2 cut(s) 152, 1004
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 3 cut(s) 50, 90, 522
BslI CCNNNNNNNGG 2 cut(s) 613, 915
BsmAI GTCTC 4 cut(s) 117, 843, 888, 918
BsmBI CGTCTC 1 cut(s) 117
BsnI GGCC 3 cut(s) 470, 525, 1127
BsoBI CYCGRG 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 2 cut(s) 152, 1004
Bsp143I GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
Bsp1720I GCTNAGC 2 cut(s) 146, 938
Bsp19I CCATGG 1 cut(s) 223
BspACI CCGC 2 cut(s) 450, 1128
BspANI GGCC 3 cut(s) 470, 525, 1127
BspCNI CTCAG 5 cut(s) 138, 259, 774, 951, 967
BspDI ATCGAT 1 cut(s) 582
BspLI GGNNCC 1 cut(s) 54
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 2 cut(s) 110, 583
BspT104I TTCGAA 1 cut(s) 802
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 1 cut(s) 223
BssMI GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
BssT1I CCWWGG 1 cut(s) 223
Bst2UI CCWGG 1 cut(s) 934
Bst4CI ACNGT 4 cut(s) 438, 509, 519, 950
Bst6I CTCTTC 2 cut(s) 387, 974
BstBAI YACGTR 1 cut(s) 72
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 1 cut(s) 1125
BstDEI CTNAG 6 cut(s) 146, 267, 272, 761, 938, 975
BstDSI CCRYGG 1 cut(s) 223
BstKTI GATC 7 cut(s) 105, 591, 829, 878, 962, 1009, 1039
BstMAI GTCTC 4 cut(s) 117, 843, 888, 918
BstMBI GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
BstMCI CGRYCG 1 cut(s) 1069
BstMWI GCNNNNNNNGC 4 cut(s) 35, 346, 673, 1136
BstNI CCWGG 1 cut(s) 934
BstSCI CCNGG 2 cut(s) 89, 932
BstV1I GCAGC 5 cut(s) 13, 220, 386, 931, 1041
BstV2I GAAGAC 4 cut(s) 269, 791, 854, 861
BstX2I RGATCY 2 cut(s) 102, 588
BstYI RGATCY 2 cut(s) 102, 588
Bsu15I ATCGAT 1 cut(s) 582
BsuRI GGCC 3 cut(s) 470, 525, 1127
BsuTUI ATCGAT 1 cut(s) 582
BtgI CCRYGG 1 cut(s) 223
BtrI CACGTC 1 cut(s) 136
BtsIMutI CAGTG 2 cut(s) 264, 882
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 1125
Cfr13I GGNCC 3 cut(s) 52, 452, 906
ClaI ATCGAT 1 cut(s) 582
Csp6I GTAC 7 cut(s) 73, 253, 400, 430, 538, 748, 972
CviAII CATG 5 cut(s) 195, 224, 298, 623, 656
CviQI GTAC 7 cut(s) 73, 253, 400, 430, 538, 748, 972
DdeI CTNAG 6 cut(s) 146, 267, 272, 761, 938, 975
DpnI GATC 7 cut(s) 104, 590, 828, 877, 961, 1008, 1038
DpnII GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
DriI GACNNNNNGTC 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 468
Eam1104I CTCTTC 2 cut(s) 387, 974
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 2 cut(s) 387, 974
Eco130I CCWWGG 1 cut(s) 223
Eco47I GGWCC 3 cut(s) 52, 452, 906
Eco57I CTGAAG 3 cut(s) 651, 866, 1139
Eco88I CYCGRG 1 cut(s) 120
EcoRII CCWGG 1 cut(s) 932
EcoT14I CCWWGG 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 223
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 5 cut(s) 198, 227, 301, 626, 659
FatI CATG 5 cut(s) 194, 223, 297, 622, 655
FauNDI CATATG 2 cut(s) 64, 358
Fnu4HI GCNGC 6 cut(s) 27, 234, 375, 920, 1030, 1128
Fsp4HI GCNGC 6 cut(s) 27, 234, 375, 920, 1030, 1128
FspBI CTAG 2 cut(s) 903, 1053
GluI GCNGC 6 cut(s) 27, 234, 375, 920, 1030, 1128
HaeIII GGCC 3 cut(s) 470, 525, 1127
HapII CCGG 3 cut(s) 50, 90, 522
Hin1II CATG 5 cut(s) 198, 227, 301, 626, 659
HincII GTYRAC 1 cut(s) 133
HindII GTYRAC 1 cut(s) 133
HinfI GANTC 2 cut(s) 619, 836
HpaII CCGG 3 cut(s) 50, 90, 522
HphI GGTGA 3 cut(s) 151, 628, 1096
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 7 cut(s) 109, 249, 513, 835, 846, 1045, 1119
Hpy188III TCNNGA 8 cut(s) 122, 333, 547, 743, 763, 963, 993, 1014
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 1 cut(s) 83
HpyAV CCTTC 2 cut(s) 536, 990
HpyCH4III ACNGT 4 cut(s) 438, 509, 519, 950
HpyCH4IV ACGT 3 cut(s) 71, 135, 1107
HpyCH4V TGCA 6 cut(s) 11, 29, 362, 458, 681, 824
HpyF10VI GCNNNNNNNGC 4 cut(s) 35, 346, 673, 1136
HpyF3I CTNAG 6 cut(s) 146, 267, 272, 761, 938, 975
HpySE526I ACGT 3 cut(s) 71, 135, 1107
Hsp92II CATG 5 cut(s) 198, 227, 301, 626, 659
Kzo9I GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
LmnI GCTCC 3 cut(s) 673, 999, 1026
Lsp1109I GCAGC 5 cut(s) 13, 220, 386, 931, 1041
LweI GCATC 1 cut(s) 766
MaeI CTAG 2 cut(s) 903, 1053
MaeII ACGT 3 cut(s) 71, 135, 1107
MaeIII GTNAC 2 cut(s) 725, 1103
MalI GATC 7 cut(s) 104, 590, 828, 877, 961, 1008, 1038
MboI GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
MflI RGATCY 2 cut(s) 102, 588
MhlI GDGCHC 2 cut(s) 152, 1004
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 1 cut(s) 805
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 3 cut(s) 424, 1023, 1075
Mox20I TGGCCA 1 cut(s) 470
MroXI GAANNNNTTC 1 cut(s) 554
MscI TGGCCA 1 cut(s) 470
MslI CAYNNNNRTG 3 cut(s) 228, 285, 382
Msp20I TGGCCA 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 667
MspI CCGG 3 cut(s) 50, 90, 522
MspR9I CCNGG 2 cut(s) 91, 934
MvaI CCWGG 1 cut(s) 934
MwoI GCNNNNNNNGC 4 cut(s) 35, 346, 673, 1136
NciI CCSGG 1 cut(s) 91
NcoI CCATGG 1 cut(s) 223
NdeI CATATG 2 cut(s) 64, 358
NdeII GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
NlaIII CATG 5 cut(s) 198, 227, 301, 626, 659
NlaIV GGNNCC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 802
PaeR7I CTCGAG 1 cut(s) 120
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 1 cut(s) 619
PkrI GCNGC 6 cut(s) 28, 235, 376, 921, 1031, 1129
PleI GAGTC 1 cut(s) 844
PpsI GAGTC 1 cut(s) 844
Ppu21I YACGTR 1 cut(s) 72
PsiI TTATAA 1 cut(s) 573
Psp6I CCWGG 1 cut(s) 932
PspGI CCWGG 1 cut(s) 932
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 3 cut(s) 52, 452, 906
PsuI RGATCY 2 cut(s) 102, 588
PvuII CAGCTG 1 cut(s) 667
RsaI GTAC 7 cut(s) 74, 254, 401, 431, 539, 749, 973
RsaNI GTAC 7 cut(s) 73, 253, 400, 430, 538, 748, 972
RseI CAYNNNNRTG 3 cut(s) 228, 285, 382
SatI GCNGC 6 cut(s) 27, 234, 375, 920, 1030, 1128
Sau3AI GATC 7 cut(s) 102, 588, 826, 875, 959, 1006, 1036
Sau96I GGNCC 3 cut(s) 52, 452, 906
ScaI AGTACT 2 cut(s) 749, 973
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 2 cut(s) 91, 934
SduI GDGCHC 2 cut(s) 152, 1004
SfaNI GCATC 1 cut(s) 766
Sfr274I CTCGAG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 802
SinI GGWCC 3 cut(s) 52, 452, 906
SlaI CTCGAG 1 cut(s) 120
SmiMI CAYNNNNRTG 3 cut(s) 228, 285, 382
SmlI CTYRAG 4 cut(s) 120, 487, 751, 889
SmoI CTYRAG 4 cut(s) 120, 487, 751, 889
Sse9I AATT 1 cut(s) 805
SsiI CCGC 2 cut(s) 450, 1128
SspMI CTAG 2 cut(s) 903, 1053
StyD4I CCNGG 2 cut(s) 89, 932
StyI CCWWGG 1 cut(s) 223
TaaI ACNGT 4 cut(s) 438, 509, 519, 950
TaiI ACGT 3 cut(s) 74, 138, 1110
TaqI TCGA 7 cut(s) 78, 121, 529, 564, 582, 802, 1065
TaqII GACCGA 3 cut(s) 866, 999, 1083
TasI AATT 1 cut(s) 805
TatI WGTACW 2 cut(s) 747, 971
TauI GCSGC 1 cut(s) 1130
TfiI GAWTC 1 cut(s) 619
TscAI CASTG 2 cut(s) 271, 889
TseI GCWGC 5 cut(s) 26, 233, 374, 919, 1029
TspDTI ATGAA 5 cut(s) 269, 611, 651, 708, 760
TspGWI ACGGA 2 cut(s) 48, 447
TspRI CASTG 2 cut(s) 271, 889
VpaK11BI GGWCC 3 cut(s) 52, 452, 906
XapI RAATTY 1 cut(s) 805
XhoI CTCGAG 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 2 cut(s) 903, 1053
ZrmI AGTACT 2 cut(s) 749, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.