MD13G1055400.v1.1

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011268
Physical Location & Seq
Reverse (-)
3872148 .. 3874390
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1055400.v1.1.491

Sequence Viewer

Length: 1143 bp
ATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTTGCATTTTTCCCACCGGACCCTCCGTCATACGACACGTACGTCGACGAACCAACGGGGAAAATGAGGATCTCCGATGTTCTTCCCCGAGACGACGTTGACGTGCTGAGGCTGAGCACAAAAAAAGGGAATGAGATTGTGGCCATGTATGTCAAAAATCCATGTGCTTCACTCACTGTTCTTTACTCCCATGGAAATGCTGCTGATCTTGGTCAGATGTACCACATTTTCACTGAGCTTAGCCTTCATCTGGGTGTCAATCTCATGGGGTACGATTATTCTGGCTATGGACAATCTTCTGGAAAGCCAAGTGAGCAGGACACATATGCAGACATAGAGGCAGCATACAAATGCCTTGAAGAGAAGTACGGTATAAAGGAGGAAGATGTCATTCTGTACGGACAGTCAGTTGGAAGCGGACCTGCTTTGGATTTGGCCGTTCGTTTGCCTAACTTGAGGGCTGTTGTTCTTCACAGTCCAATATTGTCGGGCCTCCGAGTAATGTACCATGTCAAGAAAACATTCTGGTTTGACATTTATAAGAATATCGATAAGATCCCACTTGTCAGTTGCCCAGTTTTGGTGATTCATGGAACTGAAGATGAAGTTGTGGATTCTTCCCATGGAAAACAGTTGTGGGAGCTTTGCAAAGAGAAGTATGAACCTTTGTGGCTCAAAGGAGGCAACCATTGTAACTTGGAACTCTATCCGGAATACTTGAGGCATCTCAGGAAGTTCATATCTGCCATAGAGAAATTACCCCGTCTTCGAAATTCAACAGAACAAAGTGCAGACCAATCAGAGTCTCCTCTGAAGATTGAAGACATCAGGGACAAGGCAAGATCAAGCACTGACTTAAGGGACAAGTCTAGGTCCAGCACCGGGAAGCGAGACAAGTCTAGGCTAAGCACAGACAGTAGAGAGAGATCAAGTACAAGCACTGAAAAGAGAGAAAAGTCAAGAAAGAGCACCGATCGGTCTGGAAAGTTGAGGAGCAGCACAGATCAGTCGGAGAGAGCTAGAAATAGCTTCGACCGATTGGGAGATATGGTGAGGTCAGTTGGATTGTGTAACGTTGATTGTCTGAAGCAGGCGGCTTTGGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

381

Amino Acids

42.68

Weight (kDa)

7.59

Isoelectric Point (pI)

60.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 70 - 174 3.3e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 171 3.3e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 573
AasI GACNNNNNNGTC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 742
AciI CCGC 2 cut(s) 450, 1127
AclI AACGTT 1 cut(s) 1107
AclWI GGATC 2 cut(s) 110, 583
AcoI YGGCCR 2 cut(s) 174, 468
AcsI RAATTY 1 cut(s) 805
AcuI CTGAAG 3 cut(s) 651, 866, 1139
AfaI GTAC 7 cut(s) 74, 254, 305, 401, 431, 539, 967
AfiI CCNNNNNNNGG 3 cut(s) 283, 613, 915
AflII CTTAAG 1 cut(s) 889
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 3 cut(s) 392, 810, 854
AhdI GACNNNNNGTC 1 cut(s) 58
AjiI CACGTC 1 cut(s) 136
AluBI AGCT 4 cut(s) 271, 676, 1052, 1062
AluI AGCT 4 cut(s) 271, 676, 1052, 1062
Alw21I GWGCWC 2 cut(s) 152, 1004
Alw26I GTCTC 3 cut(s) 117, 843, 918
AlwI GGATC 2 cut(s) 110, 583
Ama87I CYCGRG 1 cut(s) 120
Aor13HI TCCGGA 1 cut(s) 742
AoxI GGCC 3 cut(s) 174, 468, 523
ApeKI GCWGC 4 cut(s) 26, 233, 374, 1029
ApoI RAATTY 1 cut(s) 805
Asp700I GAANNNNTTC 1 cut(s) 554
AspS9I GGNCC 4 cut(s) 52, 452, 523, 906
AsuC2I CCSGG 1 cut(s) 916
AsuHPI GGTGA 2 cut(s) 628, 1096
AsuII TTCGAA 1 cut(s) 802
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 3 cut(s) 52, 452, 906
BalI TGGCCA 1 cut(s) 176
BbsI GAAGAC 2 cut(s) 791, 861
Bbv12I GWGCWC 2 cut(s) 152, 1004
BbvCI CCTCAGC 1 cut(s) 140
BbvI GCAGC 4 cut(s) 13, 220, 386, 1041
BceAI ACGGC 1 cut(s) 455
BcnI CCSGG 1 cut(s) 916
BcoDI GTCTC 3 cut(s) 117, 843, 918
BfaI CTAG 3 cut(s) 903, 933, 1053
BfrI CTTAAG 1 cut(s) 889
BfuAI ACCTGC 1 cut(s) 463
BisI GCNGC 5 cut(s) 27, 234, 375, 1030, 1128
BlpI GCTNAGC 3 cut(s) 146, 272, 938
BlsI GCNGC 5 cut(s) 28, 235, 376, 1031, 1129
Bme1390I CCNGG 1 cut(s) 916
Bme18I GGWCC 3 cut(s) 52, 452, 906
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 120
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 4 cut(s) 52, 452, 523, 906
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 1 cut(s) 916
BmrI ACTGGG 1 cut(s) 602
BmsI GCATC 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 602
BpiI GAAGAC 2 cut(s) 791, 861
Bpu10I CCTNAGC 1 cut(s) 140
Bpu1102I GCTNAGC 3 cut(s) 146, 272, 938
Bpu14I TTCGAA 1 cut(s) 802
BpuEI CTTGAG 2 cut(s) 508, 772
BpuMI CCSGG 1 cut(s) 916
Bsa29I ATCGAT 1 cut(s) 582
BsaAI YACGTR 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 223, 655
BsaWI WCCGGW 2 cut(s) 49, 742
BsaXI ACNNNNNCTCC 2 cut(s) 1068, 1098
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 613, 915
Bse1I ACTGG 1 cut(s) 608
BseAI TCCGGA 1 cut(s) 742
BseCI ATCGAT 1 cut(s) 582
BseDI CCNNGG 2 cut(s) 223, 655
BseLI CCNNNNNNNGG 3 cut(s) 283, 613, 915
BseMII CTCAG 4 cut(s) 131, 137, 258, 775
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 2 cut(s) 831, 1039
BseXI GCAGC 4 cut(s) 13, 220, 386, 1041
BsgI GTGCAG 1 cut(s) 843
Bsh1285I CGRYCG 2 cut(s) 1009, 1069
BshFI GGCC 3 cut(s) 176, 470, 525
BshVI ATCGAT 1 cut(s) 582
BsiEI CGRYCG 2 cut(s) 1009, 1069
BsiHKAI GWGCWC 2 cut(s) 152, 1004
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 3 cut(s) 50, 743, 915
BsiWI CGTACG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 878, 908
BslI CCNNNNNNNGG 3 cut(s) 283, 613, 915
BsmAI GTCTC 3 cut(s) 117, 843, 918
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 878, 908
BsnI GGCC 3 cut(s) 176, 470, 525
BsoBI CYCGRG 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 802
Bsp1286I GDGCHC 2 cut(s) 152, 1004
Bsp13I TCCGGA 1 cut(s) 742
Bsp143I GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
Bsp1720I GCTNAGC 3 cut(s) 146, 272, 938
Bsp19I CCATGG 2 cut(s) 223, 655
BspACI CCGC 2 cut(s) 450, 1127
BspANI GGCC 3 cut(s) 176, 470, 525
BspCNI CTCAG 4 cut(s) 132, 138, 259, 774
BspDI ATCGAT 1 cut(s) 582
BspEI TCCGGA 1 cut(s) 742
BspLI GGNNCC 1 cut(s) 54
BspMI ACCTGC 1 cut(s) 463
BspPI GGATC 2 cut(s) 110, 583
BspT104I TTCGAA 1 cut(s) 802
BspTI CTTAAG 1 cut(s) 889
BsrI ACTGG 1 cut(s) 608
BssECI CCNNGG 2 cut(s) 223, 655
BssMI GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
BssT1I CCWWGG 2 cut(s) 223, 655
Bst4CI ACNGT 6 cut(s) 211, 404, 438, 509, 666, 950
Bst6I CTCTTC 1 cut(s) 387
BstAFI CTTAAG 1 cut(s) 889
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 72
BstBI TTCGAA 1 cut(s) 802
BstC8I GCNNGC 1 cut(s) 1125
BstDEI CTNAG 6 cut(s) 140, 146, 267, 272, 761, 938
BstDSI CCRYGG 2 cut(s) 223, 655
BstKTI GATC 7 cut(s) 105, 241, 591, 878, 962, 1009, 1039
BstMAI GTCTC 3 cut(s) 117, 843, 918
BstMBI GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
BstMCI CGRYCG 2 cut(s) 1009, 1069
BstMWI GCNNNNNNNGC 3 cut(s) 35, 346, 1136
BstSCI CCNGG 1 cut(s) 914
BstV1I GCAGC 4 cut(s) 13, 220, 386, 1041
BstV2I GAAGAC 2 cut(s) 791, 861
BstX2I RGATCY 2 cut(s) 102, 588
BstYI RGATCY 2 cut(s) 102, 588
Bsu15I ATCGAT 1 cut(s) 582
BsuRI GGCC 3 cut(s) 176, 470, 525
BsuTUI ATCGAT 1 cut(s) 582
BtgI CCRYGG 2 cut(s) 223, 655
BtrI CACGTC 1 cut(s) 136
BtsIMutI CAGTG 4 cut(s) 207, 264, 882, 972
BveI ACCTGC 1 cut(s) 463
Cac8I GCNNGC 1 cut(s) 1125
Cfr13I GGNCC 4 cut(s) 52, 452, 523, 906
ClaI ATCGAT 1 cut(s) 582
Csp6I GTAC 7 cut(s) 73, 253, 304, 400, 430, 538, 966
CviAII CATG 7 cut(s) 178, 195, 224, 298, 542, 623, 656
CviQI GTAC 7 cut(s) 73, 253, 304, 400, 430, 538, 966
DdeI CTNAG 6 cut(s) 140, 146, 267, 272, 761, 938
DpnI GATC 7 cut(s) 104, 240, 590, 877, 961, 1008, 1038
DpnII GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
DrdI GACNNNNNNGTC 1 cut(s) 74
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 2 cut(s) 174, 468
Eam1104I CTCTTC 1 cut(s) 387
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 1 cut(s) 387
Eco130I CCWWGG 2 cut(s) 223, 655
Eco47I GGWCC 3 cut(s) 52, 452, 906
Eco57I CTGAAG 3 cut(s) 651, 866, 1139
Eco88I CYCGRG 1 cut(s) 120
EcoT14I CCWWGG 2 cut(s) 223, 655
ErhI CCWWGG 2 cut(s) 223, 655
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 7 cut(s) 181, 198, 227, 301, 545, 626, 659
FaqI GGGAC 2 cut(s) 878, 908
FatI CATG 7 cut(s) 177, 194, 223, 297, 541, 622, 655
FauNDI CATATG 1 cut(s) 358
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 5 cut(s) 27, 234, 375, 1030, 1128
Fsp4HI GCNGC 5 cut(s) 27, 234, 375, 1030, 1128
FspBI CTAG 3 cut(s) 903, 933, 1053
GluI GCNGC 5 cut(s) 27, 234, 375, 1030, 1128
HaeIII GGCC 3 cut(s) 176, 470, 525
HapII CCGG 3 cut(s) 50, 743, 915
Hin1II CATG 7 cut(s) 181, 198, 227, 301, 545, 626, 659
HincII GTYRAC 2 cut(s) 79, 133
HindII GTYRAC 2 cut(s) 79, 133
HinfI GANTC 3 cut(s) 619, 647, 836
HpaII CCGG 3 cut(s) 50, 743, 915
HphI GGTGA 2 cut(s) 628, 1096
Hpy166II GTNNAC 2 cut(s) 79, 133
Hpy188I TCNGA 7 cut(s) 109, 249, 530, 835, 846, 1045, 1119
Hpy188III TCNNGA 6 cut(s) 333, 547, 743, 763, 993, 1014
Hpy8I GTNNAC 2 cut(s) 79, 133
Hpy99I CGWCG 3 cut(s) 80, 83, 131
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 6 cut(s) 211, 404, 438, 509, 666, 950
HpyCH4IV ACGT 5 cut(s) 71, 75, 129, 135, 1107
HpyCH4V TGCA 6 cut(s) 11, 29, 38, 362, 681, 824
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 346, 1136
HpyF3I CTNAG 6 cut(s) 140, 146, 267, 272, 761, 938
HpySE526I ACGT 5 cut(s) 71, 75, 129, 135, 1107
Hsp92II CATG 7 cut(s) 181, 198, 227, 301, 545, 626, 659
Kpn2I TCCGGA 1 cut(s) 742
Kzo9I GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
LmnI GCTCC 2 cut(s) 673, 1026
Lsp1109I GCAGC 4 cut(s) 13, 220, 386, 1041
LweI GCATC 1 cut(s) 766
MaeI CTAG 3 cut(s) 903, 933, 1053
MaeII ACGT 5 cut(s) 71, 75, 129, 135, 1107
MaeIII GTNAC 2 cut(s) 725, 1103
MalI GATC 7 cut(s) 104, 240, 590, 877, 961, 1008, 1038
MboI GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
MflI RGATCY 2 cut(s) 102, 588
MhlI GDGCHC 2 cut(s) 152, 1004
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 2 cut(s) 788, 805
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 845
MmeI TCCRAC 3 cut(s) 424, 1023, 1075
Mox20I TGGCCA 1 cut(s) 176
MroI TCCGGA 1 cut(s) 742
MroXI GAANNNNTTC 1 cut(s) 554
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 890
MslI CAYNNNNRTG 3 cut(s) 228, 285, 382
Msp20I TGGCCA 1 cut(s) 176
MspCI CTTAAG 1 cut(s) 889
MspI CCGG 3 cut(s) 50, 743, 915
MspR9I CCNGG 1 cut(s) 916
MwoI GCNNNNNNNGC 3 cut(s) 35, 346, 1136
NciI CCSGG 1 cut(s) 916
NcoI CCATGG 2 cut(s) 223, 655
NdeI CATATG 1 cut(s) 358
NdeII GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
NlaIII CATG 7 cut(s) 181, 198, 227, 301, 545, 626, 659
NlaIV GGNNCC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 802
PcsI WCGNNNNNNNCGW 2 cut(s) 72, 132
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 619, 647
Pfl23II CGTACG 1 cut(s) 72
PkrI GCNGC 5 cut(s) 28, 235, 376, 1031, 1129
Ple19I CGATCG 1 cut(s) 1009
PleI GAGTC 1 cut(s) 844
PpsI GAGTC 1 cut(s) 844
Ppu21I YACGTR 1 cut(s) 72
PsiI TTATAA 1 cut(s) 573
Psp1406I AACGTT 1 cut(s) 1107
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 4 cut(s) 52, 452, 523, 906
PsuI RGATCY 2 cut(s) 102, 588
PvuI CGATCG 1 cut(s) 1009
RsaI GTAC 7 cut(s) 74, 254, 305, 401, 431, 539, 967
RsaNI GTAC 7 cut(s) 73, 253, 304, 400, 430, 538, 966
RseI CAYNNNNRTG 3 cut(s) 228, 285, 382
SalI GTCGAC 1 cut(s) 77
SaqAI TTAA 1 cut(s) 890
SatI GCNGC 5 cut(s) 27, 234, 375, 1030, 1128
Sau3AI GATC 7 cut(s) 102, 238, 588, 875, 959, 1006, 1036
Sau96I GGNCC 4 cut(s) 52, 452, 523, 906
SchI GAGTC 1 cut(s) 845
ScrFI CCNGG 1 cut(s) 916
SduI GDGCHC 2 cut(s) 152, 1004
SfaNI GCATC 1 cut(s) 766
SfuI TTCGAA 1 cut(s) 802
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 3 cut(s) 52, 452, 906
SmiMI CAYNNNNRTG 3 cut(s) 228, 285, 382
SmlI CTYRAG 3 cut(s) 487, 751, 889
SmoI CTYRAG 3 cut(s) 487, 751, 889
Sse9I AATT 2 cut(s) 788, 805
SsiI CCGC 2 cut(s) 450, 1127
SspI AATATT 1 cut(s) 516
SspMI CTAG 3 cut(s) 903, 933, 1053
StyD4I CCNGG 1 cut(s) 914
StyI CCWWGG 2 cut(s) 223, 655
TaaI ACNGT 6 cut(s) 211, 404, 438, 509, 666, 950
TaiI ACGT 5 cut(s) 74, 78, 132, 138, 1110
TaqI TCGA 4 cut(s) 78, 582, 802, 1065
TaqII GACCGA 2 cut(s) 999, 1083
TasI AATT 2 cut(s) 788, 805
TatI WGTACW 1 cut(s) 965
TauI GCSGC 1 cut(s) 1130
TfiI GAWTC 2 cut(s) 619, 647
Tru1I TTAA 1 cut(s) 890
Tru9I TTAA 1 cut(s) 890
TscAI CASTG 4 cut(s) 214, 271, 889, 979
TseI GCWGC 4 cut(s) 26, 233, 374, 1029
TspDTI ATGAA 5 cut(s) 269, 611, 651, 708, 760
TspGWI ACGGA 2 cut(s) 48, 447
TspRI CASTG 4 cut(s) 214, 271, 889, 979
Vha464I CTTAAG 1 cut(s) 889
VpaK11BI GGWCC 3 cut(s) 52, 452, 906
XapI RAATTY 1 cut(s) 805
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 3 cut(s) 903, 933, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.