pycom13g04900

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011268
Physical Location & Seq
Reverse (-)
3286641 .. 3288888
2248 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g04900.3

Sequence Viewer

Length: 1101 bp
ATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTTGCATTTTTCCCACCGGACCCTCCGTCATATGACACGTACGTCGACGAACCAACCGGGAAAATGAGGATCTCCAATGTTCTTCCCCGAGACGACGTTGATGTGGTGAGGCTGAGCACAAAAAAAGGGAATGAGATTGTGGCCATGTATGTCAAAAATCCATGTGCTTCACTCACTCTTCTGTACTCCCATGGAAACGCTGCTGATCTTGGTCAGATGTACCACATTTTCACTGAGCTTAGCCTTCATCTGGGTGTCAATCTCATGGGTGAGCAGGACACATATGCAGACATAGAGGCAGCATACAAATGCCTTGAAGAGAAGTTCGGTGTAAAGGAGGAAGATGTCATTCTGTACGGACAGTCAGTTGGAAGCGGACCTGCTTTGGATTTGGCCGTTCGTTTGCCTAACTTGAGGGCTGTTGTTCTTCACAGTCCAATATTGTCCGGCCTCCGAGTAATGTACCATGTCAAGAAAACATTCTGGTTTGACATTTACAAGAATATCGATAAGATCCCACTTGTCAGTTGCCCAGTTTTGGTGATACATGGAACTGAAGATGAAGTTGTGGATTTCTCCCATGGAAAACAGCTGTGGGAGCTTTGCAAAGAGAAGTATGAACCTTTGTGGCTCAAAGGAGGCAACCATTGTAACTTGGAACTCTATCCGGAATACTTGAGGCATCTCAGGAAGTTCATATCTGCCATAGAGAAATTACCCCGTCTTCGAAATTCAACCGAACAAAGCGCAGATCAATCAGAGTCTCCTCTGAAGATTGAAGACATCGGGGACAAGGCAAGATCAAGCACTGACTTAAGGGACAAGTCTAGGTCCAGCACCGGGAAGCGAGACAAGTCTAGGCTAAGCACAGACAGTAGAGAAAGATCAAGTACAAGCACTGAAAAGAGAGAGAAGTCAAGAAAGAGCACCGATCGGTCTGGAAAGTTGAGGAGCAGCACAGATCAGTCGGAGAGGGCTAGAAATAGCTTCGACCGATTGGGAGACATGGTGAGGTCAGTTGGATTGTGTAACGTTGATTGTTTGAAGCAGGCGGCTTTGGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

367

Amino Acids

41.13

Weight (kDa)

7.6

Isoelectric Point (pI)

58.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 421
AccI GTMKAC 1 cut(s) 78
AccIII TCCGGA 1 cut(s) 700
AciI CCGC 2 cut(s) 408, 1085
AclI AACGTT 1 cut(s) 1065
AclWI GGATC 2 cut(s) 110, 541
AcoI YGGCCR 2 cut(s) 174, 426
AcsI RAATTY 1 cut(s) 763
AcuI CTGAAG 2 cut(s) 609, 824
AfaI GTAC 6 cut(s) 74, 218, 254, 389, 497, 925
AfiI CCNNNNNNNGG 3 cut(s) 283, 571, 873
AflII CTTAAG 1 cut(s) 847
AflIII ACRYGT 1 cut(s) 69
AgsI TTSAA 4 cut(s) 350, 768, 812, 1078
AhdI GACNNNNNGTC 1 cut(s) 58
AluBI AGCT 4 cut(s) 271, 625, 634, 1020
AluI AGCT 4 cut(s) 271, 625, 634, 1020
Alw21I GWGCWC 2 cut(s) 152, 962
Alw26I GTCTC 4 cut(s) 117, 801, 876, 1029
AlwI GGATC 2 cut(s) 110, 541
Ama87I CYCGRG 1 cut(s) 120
Aor13HI TCCGGA 1 cut(s) 700
AoxI GGCC 3 cut(s) 174, 426, 481
ApeKI GCWGC 4 cut(s) 26, 233, 332, 987
ApoI RAATTY 1 cut(s) 763
Asp700I GAANNNNTTC 1 cut(s) 512
AspLEI GCGC 1 cut(s) 782
AspS9I GGNCC 3 cut(s) 52, 410, 864
AsuC2I CCSGG 2 cut(s) 91, 874
AsuHPI GGTGA 4 cut(s) 151, 314, 586, 1054
AsuII TTCGAA 1 cut(s) 760
AvaI CYCGRG 1 cut(s) 120
AvaII GGWCC 3 cut(s) 52, 410, 864
BalI TGGCCA 1 cut(s) 176
BbsI GAAGAC 2 cut(s) 749, 819
Bbv12I GWGCWC 2 cut(s) 152, 962
BbvI GCAGC 4 cut(s) 13, 220, 344, 999
BceAI ACGGC 1 cut(s) 413
BcnI CCSGG 2 cut(s) 91, 874
BcoDI GTCTC 4 cut(s) 117, 801, 876, 1029
BfaI CTAG 3 cut(s) 861, 891, 1011
BfrI CTTAAG 1 cut(s) 847
BfuAI ACCTGC 1 cut(s) 421
BisI GCNGC 5 cut(s) 27, 234, 333, 988, 1086
BlpI GCTNAGC 3 cut(s) 146, 272, 896
BlsI GCNGC 5 cut(s) 28, 235, 334, 989, 1087
Bme1390I CCNGG 2 cut(s) 91, 874
Bme18I GGWCC 3 cut(s) 52, 410, 864
BmeRI GACNNNNNGTC 1 cut(s) 58
BmeT110I CYCGRG 1 cut(s) 120
BmgT120I GGNCC 3 cut(s) 52, 410, 864
BmiI GGNNCC 1 cut(s) 54
BmrFI CCNGG 2 cut(s) 91, 874
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 1 cut(s) 724
BmuI ACTGGG 1 cut(s) 560
BpiI GAAGAC 2 cut(s) 749, 819
Bpu1102I GCTNAGC 3 cut(s) 146, 272, 896
Bpu14I TTCGAA 1 cut(s) 760
BpuEI CTTGAG 2 cut(s) 466, 730
BpuMI CCSGG 2 cut(s) 91, 874
Bsa29I ATCGAT 1 cut(s) 540
BsaAI YACGTR 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 223, 613
BsaWI WCCGGW 2 cut(s) 49, 700
BsaXI ACNNNNNCTCC 2 cut(s) 1026, 1056
Bsc4I CCNNNNNNNGG 3 cut(s) 283, 571, 873
Bse1I ACTGG 1 cut(s) 566
BseAI TCCGGA 1 cut(s) 700
BseCI ATCGAT 1 cut(s) 540
BseDI CCNNGG 2 cut(s) 223, 613
BseLI CCNNNNNNNGG 3 cut(s) 283, 571, 873
BseMII CTCAG 3 cut(s) 137, 258, 733
BseNI ACTGG 1 cut(s) 566
BseRI GAGGAG 2 cut(s) 789, 997
BseXI GCAGC 4 cut(s) 13, 220, 344, 999
Bsh1285I CGRYCG 2 cut(s) 967, 1027
BshFI GGCC 3 cut(s) 176, 428, 483
BshVI ATCGAT 1 cut(s) 540
BsiEI CGRYCG 2 cut(s) 967, 1027
BsiHKAI GWGCWC 2 cut(s) 152, 962
BsiHKCI CYCGRG 1 cut(s) 120
BsiSI CCGG 5 cut(s) 50, 90, 480, 701, 873
BsiWI CGTACG 1 cut(s) 72
BslFI GGGAC 2 cut(s) 836, 866
BslI CCNNNNNNNGG 3 cut(s) 283, 571, 873
BsmAI GTCTC 4 cut(s) 117, 801, 876, 1029
BsmBI CGTCTC 1 cut(s) 117
BsmFI GGGAC 2 cut(s) 836, 866
BsnI GGCC 3 cut(s) 176, 428, 483
BsoBI CYCGRG 1 cut(s) 120
Bsp119I TTCGAA 1 cut(s) 760
Bsp1286I GDGCHC 2 cut(s) 152, 962
Bsp13I TCCGGA 1 cut(s) 700
Bsp143I GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
Bsp1720I GCTNAGC 3 cut(s) 146, 272, 896
Bsp19I CCATGG 2 cut(s) 223, 613
BspACI CCGC 2 cut(s) 408, 1085
BspANI GGCC 3 cut(s) 176, 428, 483
BspCNI CTCAG 3 cut(s) 138, 259, 732
BspDI ATCGAT 1 cut(s) 540
BspEI TCCGGA 1 cut(s) 700
BspLI GGNNCC 1 cut(s) 54
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 2 cut(s) 110, 541
BspT104I TTCGAA 1 cut(s) 760
BspTI CTTAAG 1 cut(s) 847
BsrI ACTGG 1 cut(s) 566
BssECI CCNNGG 2 cut(s) 223, 613
BssMI GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
BssT1I CCWWGG 2 cut(s) 223, 613
Bst4CI ACNGT 3 cut(s) 396, 467, 908
Bst6I CTCTTC 2 cut(s) 216, 345
BstAFI CTTAAG 1 cut(s) 847
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 72
BstBI TTCGAA 1 cut(s) 760
BstC8I GCNNGC 1 cut(s) 1083
BstDEI CTNAG 5 cut(s) 146, 267, 272, 719, 896
BstDSI CCRYGG 2 cut(s) 223, 613
BstHHI GCGC 1 cut(s) 782
BstKTI GATC 8 cut(s) 105, 241, 549, 787, 836, 920, 967, 997
BstMAI GTCTC 4 cut(s) 117, 801, 876, 1029
BstMBI GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
BstMCI CGRYCG 2 cut(s) 967, 1027
BstMWI GCNNNNNNNGC 3 cut(s) 35, 631, 1094
BstSCI CCNGG 2 cut(s) 89, 872
BstV1I GCAGC 4 cut(s) 13, 220, 344, 999
BstV2I GAAGAC 2 cut(s) 749, 819
BstX2I RGATCY 2 cut(s) 102, 546
BstYI RGATCY 2 cut(s) 102, 546
Bsu15I ATCGAT 1 cut(s) 540
BsuRI GGCC 3 cut(s) 176, 428, 483
BsuTUI ATCGAT 1 cut(s) 540
BtgI CCRYGG 2 cut(s) 223, 613
BtsIMutI CAGTG 3 cut(s) 264, 840, 930
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 1 cut(s) 1083
CfoI GCGC 1 cut(s) 782
Cfr13I GGNCC 3 cut(s) 52, 410, 864
ClaI ATCGAT 1 cut(s) 540
Csp6I GTAC 6 cut(s) 73, 217, 253, 388, 496, 924
CviAII CATG 8 cut(s) 178, 195, 224, 298, 500, 581, 614, 1039
CviQI GTAC 6 cut(s) 73, 217, 253, 388, 496, 924
DdeI CTNAG 5 cut(s) 146, 267, 272, 719, 896
DpnI GATC 8 cut(s) 104, 240, 548, 786, 835, 919, 966, 996
DpnII GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
DrdI GACNNNNNNGTC 1 cut(s) 74
DriI GACNNNNNGTC 1 cut(s) 58
DseDI GACNNNNNNGTC 1 cut(s) 74
EaeI YGGCCR 2 cut(s) 174, 426
Eam1104I CTCTTC 2 cut(s) 216, 345
Eam1105I GACNNNNNGTC 1 cut(s) 58
EarI CTCTTC 2 cut(s) 216, 345
Eco130I CCWWGG 2 cut(s) 223, 613
Eco47I GGWCC 3 cut(s) 52, 410, 864
Eco57I CTGAAG 2 cut(s) 609, 824
Eco88I CYCGRG 1 cut(s) 120
EcoT14I CCWWGG 2 cut(s) 223, 613
ErhI CCWWGG 2 cut(s) 223, 613
Esp3I CGTCTC 1 cut(s) 117
FaeI CATG 8 cut(s) 181, 198, 227, 301, 503, 584, 617, 1042
FaqI GGGAC 2 cut(s) 836, 866
FatI CATG 8 cut(s) 177, 194, 223, 297, 499, 580, 613, 1038
FauNDI CATATG 2 cut(s) 64, 316
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 5 cut(s) 27, 234, 333, 988, 1086
Fsp4HI GCNGC 5 cut(s) 27, 234, 333, 988, 1086
FspBI CTAG 3 cut(s) 861, 891, 1011
GlaI GCGC 1 cut(s) 781
GluI GCNGC 5 cut(s) 27, 234, 333, 988, 1086
HaeIII GGCC 3 cut(s) 176, 428, 483
HapII CCGG 5 cut(s) 50, 90, 480, 701, 873
HhaI GCGC 1 cut(s) 782
Hin1II CATG 8 cut(s) 181, 198, 227, 301, 503, 584, 617, 1042
Hin6I GCGC 1 cut(s) 780
HinP1I GCGC 1 cut(s) 780
HincII GTYRAC 1 cut(s) 79
HindII GTYRAC 1 cut(s) 79
HinfI GANTC 1 cut(s) 794
HpaII CCGG 5 cut(s) 50, 90, 480, 701, 873
HphI GGTGA 4 cut(s) 151, 314, 586, 1054
Hpy166II GTNNAC 1 cut(s) 79
Hpy188I TCNGA 5 cut(s) 249, 488, 793, 804, 1003
Hpy188III TCNNGA 5 cut(s) 505, 701, 721, 951, 972
Hpy8I GTNNAC 1 cut(s) 79
Hpy99I CGWCG 3 cut(s) 80, 83, 131
HpyAV CCTTC 1 cut(s) 287
HpyCH4III ACNGT 3 cut(s) 396, 467, 908
HpyCH4IV ACGT 4 cut(s) 71, 75, 129, 1065
HpyCH4V TGCA 5 cut(s) 11, 29, 38, 320, 639
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 631, 1094
HpyF3I CTNAG 5 cut(s) 146, 267, 272, 719, 896
HpySE526I ACGT 4 cut(s) 71, 75, 129, 1065
Hsp92II CATG 8 cut(s) 181, 198, 227, 301, 503, 584, 617, 1042
HspAI GCGC 1 cut(s) 780
Kpn2I TCCGGA 1 cut(s) 700
Kzo9I GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
LmnI GCTCC 2 cut(s) 631, 984
Lsp1109I GCAGC 4 cut(s) 13, 220, 344, 999
LweI GCATC 1 cut(s) 724
MaeI CTAG 3 cut(s) 861, 891, 1011
MaeII ACGT 4 cut(s) 71, 75, 129, 1065
MaeIII GTNAC 2 cut(s) 683, 1061
MalI GATC 8 cut(s) 104, 240, 548, 786, 835, 919, 966, 996
MboI GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
MboII GAAGA 9 cut(s) 107, 203, 362, 386, 452, 602, 749, 817, 824
MflI RGATCY 2 cut(s) 102, 546
MhlI GDGCHC 2 cut(s) 152, 962
MlsI TGGCCA 1 cut(s) 176
MluCI AATT 2 cut(s) 746, 763
MluNI TGGCCA 1 cut(s) 176
MlyI GAGTC 1 cut(s) 803
MmeI TCCRAC 3 cut(s) 382, 981, 1033
Mox20I TGGCCA 1 cut(s) 176
MroI TCCGGA 1 cut(s) 700
MroXI GAANNNNTTC 1 cut(s) 512
MscI TGGCCA 1 cut(s) 176
MseI TTAA 1 cut(s) 848
MslI CAYNNNNRTG 2 cut(s) 285, 340
Msp20I TGGCCA 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 625
MspCI CTTAAG 1 cut(s) 847
MspI CCGG 5 cut(s) 50, 90, 480, 701, 873
MspR9I CCNGG 2 cut(s) 91, 874
MwoI GCNNNNNNNGC 3 cut(s) 35, 631, 1094
NciI CCSGG 2 cut(s) 91, 874
NcoI CCATGG 2 cut(s) 223, 613
NdeI CATATG 2 cut(s) 64, 316
NdeII GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
NlaIII CATG 8 cut(s) 181, 198, 227, 301, 503, 584, 617, 1042
NlaIV GGNNCC 1 cut(s) 54
NspV TTCGAA 1 cut(s) 760
PdmI GAANNNNTTC 1 cut(s) 512
Pfl23II CGTACG 1 cut(s) 72
PkrI GCNGC 5 cut(s) 28, 235, 334, 989, 1087
Ple19I CGATCG 1 cut(s) 967
PleI GAGTC 1 cut(s) 802
PpsI GAGTC 1 cut(s) 802
Ppu21I YACGTR 1 cut(s) 72
Psp1406I AACGTT 1 cut(s) 1065
PspLI CGTACG 1 cut(s) 72
PspN4I GGNNCC 1 cut(s) 54
PspPI GGNCC 3 cut(s) 52, 410, 864
PsuI RGATCY 2 cut(s) 102, 546
PvuI CGATCG 1 cut(s) 967
PvuII CAGCTG 1 cut(s) 625
RsaI GTAC 6 cut(s) 74, 218, 254, 389, 497, 925
RsaNI GTAC 6 cut(s) 73, 217, 253, 388, 496, 924
RseI CAYNNNNRTG 2 cut(s) 285, 340
SalI GTCGAC 1 cut(s) 77
SaqAI TTAA 1 cut(s) 848
SatI GCNGC 5 cut(s) 27, 234, 333, 988, 1086
Sau3AI GATC 8 cut(s) 102, 238, 546, 784, 833, 917, 964, 994
Sau96I GGNCC 3 cut(s) 52, 410, 864
SchI GAGTC 1 cut(s) 803
ScrFI CCNGG 2 cut(s) 91, 874
SduI GDGCHC 2 cut(s) 152, 962
SfaNI GCATC 1 cut(s) 724
SfuI TTCGAA 1 cut(s) 760
SgrDI CGTCGACG 1 cut(s) 77
SinI GGWCC 3 cut(s) 52, 410, 864
SmiMI CAYNNNNRTG 2 cut(s) 285, 340
SmlI CTYRAG 3 cut(s) 445, 709, 847
SmoI CTYRAG 3 cut(s) 445, 709, 847
Sse9I AATT 2 cut(s) 746, 763
SsiI CCGC 2 cut(s) 408, 1085
SspI AATATT 1 cut(s) 474
SspMI CTAG 3 cut(s) 861, 891, 1011
StyD4I CCNGG 2 cut(s) 89, 872
StyI CCWWGG 2 cut(s) 223, 613
TaaI ACNGT 3 cut(s) 396, 467, 908
TaiI ACGT 4 cut(s) 74, 78, 132, 1068
TaqI TCGA 4 cut(s) 78, 540, 760, 1023
TaqII GACCGA 2 cut(s) 957, 1041
TasI AATT 2 cut(s) 746, 763
TatI WGTACW 2 cut(s) 216, 923
TauI GCSGC 1 cut(s) 1088
Tru1I TTAA 1 cut(s) 848
Tru9I TTAA 1 cut(s) 848
TscAI CASTG 3 cut(s) 271, 847, 937
TseI GCWGC 4 cut(s) 26, 233, 332, 987
TspDTI ATGAA 4 cut(s) 269, 609, 666, 718
TspGWI ACGGA 2 cut(s) 48, 405
TspRI CASTG 3 cut(s) 271, 847, 937
Vha464I CTTAAG 1 cut(s) 847
VpaK11BI GGWCC 3 cut(s) 52, 410, 864
XapI RAATTY 1 cut(s) 763
XmiI GTMKAC 1 cut(s) 78
XmnI GAANNNNTTC 1 cut(s) 512
XspI CTAG 3 cut(s) 861, 891, 1011
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.