pycom16g04720

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0011268
Physical Location & Seq
Reverse (-)
3039218 .. 3041616
2399 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g04720.1

Sequence Viewer

Length: 1209 bp
ATGGAAAACCTTCCAAACTTTACTCTGCACCACAATTCTCTCCCAAAATTTTCCACGGATCAAGAAATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTCGCATTCTTCCCACCGGACCCTCCGTCATATGACACGTACATCGACGAACCAACCGGGAAAATGAGGATCTCCGATGTTCTTCCTCGAGACGATGTTGACGTGGTGAAGCTGAGCACAAAAAAAGGGAATGAGATCGTGGCTATGTATGTCAAAAATCCAAATGCTTCACTCACTCTGCTTTACTCCCATGGAAATGCTGCTGATATTGGTCAGATGTACCACATTTTCACTGAGCTTAGTCTTCATCTGGGTGTCAATCTCATGGGCTACGATTATTCTGGCTATGGACAATCTTCTGGAAAGCCAAGTGAGCAGGACACATATGCAGACATAGAGGCAGCTTACAAATGCCTTGAAGAGAAGTACGGCATAAAGGAGGAAGACGTTATTTTGTACGGACAGTCAGTTGGTAGCGGACCGGCTTTGGATTTGGCCATTCGTTTGCCTAACTTGAGGGGTGTTGTTCTTCACAGTCCGATACTGTCCGGCCTTCGAGTGATGTATCCTGTCAAGAAAACATTCTGGTTCGACATTTATAAGAATATCGATAAGATCCCACATGTCAGTTGCCCAGTTTTGGTGATTCATGGAACTGAAGATGAAGTTGTGGATTTCTCGCATGGAAAACAGCTGTGGGAGCTTTGCAAAGAGAAATATGAACCCTTGTGGCTCAAGGGGGGCAACCATTGTAACTTGGAACTCTATCCAGAGTACTTGAGGCATCTCAGGAAGTTCATATCTGCCATAGAGAAACTACCCCGTCTTCGAAATTCAACCGAACAGAATGCAGATCAATCGGAGTCACCTCTGAAGACTGAAGACAACAAGGACAAGGCAAGATCAAGCACTGACTTGAGGGACAAGTCTAGGTCCAGCATGGGGCAGCGAGACAAGTCTAGGCTCAGCACAGACAGTAAAGAGAGATCAAGAACAAGCACTGAGAAAAGAGAGAAGTCAAGAAAGAGCACAGATCGGTCTGGAAAGGCAAGGAGCAGCATAGATCAGTCGGAGAGAGCTAGAAATAGCTTCGACCGATTGGGAGATATGGTGAGGTCAGTTGGATTGTGTAACGTAGATTGTCTGAAGCAGGCCGTGTTAGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

403

Amino Acids

45.36

Weight (kDa)

6.84

Isoelectric Point (pI)

56.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 92 - 197 1.1e-10 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 93 - 193 7.2e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
AciI CCGC 1 cut(s) 516
AclWI GGATC 3 cut(s) 66, 176, 649
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 2 cut(s) 47, 871
AcuI CTGAAG 4 cut(s) 717, 932, 939, 1205
AfaI GTAC 5 cut(s) 140, 320, 467, 497, 815
AfiI CCNNNNNNNGG 2 cut(s) 679, 981
AflIII ACRYGT 2 cut(s) 135, 661
AgsI TTSAA 2 cut(s) 458, 876
AhdI GACNNNNNGTC 1 cut(s) 124
AjiI CACGTC 1 cut(s) 202
AluBI AGCT 7 cut(s) 211, 337, 443, 733, 742, 1118, 1128
AluI AGCT 7 cut(s) 211, 337, 443, 733, 742, 1118, 1128
Alw21I GWGCWC 2 cut(s) 218, 1070
Alw26I GTCTC 2 cut(s) 183, 984
AlwI GGATC 3 cut(s) 66, 176, 649
Ama87I CYCGRG 1 cut(s) 186
AoxI GGCC 3 cut(s) 534, 589, 1191
ApeKI GCWGC 5 cut(s) 92, 299, 440, 985, 1095
ApoI RAATTY 2 cut(s) 47, 871
Asp700I GAANNNNTTC 2 cut(s) 9, 620
AspS9I GGNCC 3 cut(s) 118, 518, 972
AsuC2I CCSGG 1 cut(s) 157
AsuHPI GGTGA 4 cut(s) 217, 694, 897, 1162
AsuII TTCGAA 1 cut(s) 868
AvaI CYCGRG 1 cut(s) 186
AvaII GGWCC 3 cut(s) 118, 518, 972
BalI TGGCCA 1 cut(s) 536
BbsI GAAGAC 5 cut(s) 335, 489, 857, 920, 927
Bbv12I GWGCWC 2 cut(s) 218, 1070
BbvI GCAGC 5 cut(s) 79, 286, 452, 997, 1107
BceAI ACGGC 2 cut(s) 484, 1178
BcgI CGANNNNNNTGC 4 cut(s) 869, 879, 903, 913
BciVI GTATCC 1 cut(s) 615
BcnI CCSGG 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 183, 984
BfaI CTAG 3 cut(s) 969, 999, 1119
BfuI GTATCC 1 cut(s) 615
BisI GCNGC 5 cut(s) 93, 300, 441, 986, 1096
BlpI GCTNAGC 2 cut(s) 212, 1004
BlsI GCNGC 5 cut(s) 94, 301, 442, 987, 1097
BmcAI AGTACT 1 cut(s) 815
Bme1390I CCNGG 1 cut(s) 157
Bme18I GGWCC 3 cut(s) 118, 518, 972
BmeRI GACNNNNNGTC 1 cut(s) 124
BmeT110I CYCGRG 1 cut(s) 186
BmgBI CACGTC 1 cut(s) 202
BmgT120I GGNCC 3 cut(s) 118, 518, 972
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 157
BmrI ACTGGG 1 cut(s) 668
BmsI GCATC 1 cut(s) 832
BmuI ACTGGG 1 cut(s) 668
BpiI GAAGAC 5 cut(s) 335, 489, 857, 920, 927
Bpu1102I GCTNAGC 2 cut(s) 212, 1004
Bpu14I TTCGAA 1 cut(s) 868
BpuEI CTTGAG 4 cut(s) 574, 758, 838, 976
BpuMI CCSGG 1 cut(s) 157
Bsa29I ATCGAT 1 cut(s) 648
BsaAI YACGTR 1 cut(s) 138
BsaJI CCNNGG 2 cut(s) 54, 289
BsaWI WCCGGW 1 cut(s) 115
BsaXI ACNNNNNCTCC 2 cut(s) 1134, 1164
Bsc4I CCNNNNNNNGG 2 cut(s) 679, 981
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 1 cut(s) 674
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 2 cut(s) 54, 289
BseLI CCNNNNNNNGG 2 cut(s) 679, 981
BseMII CTCAG 5 cut(s) 203, 324, 841, 1018, 1032
BseNI ACTGG 1 cut(s) 674
BseXI GCAGC 5 cut(s) 79, 286, 452, 997, 1107
BsgI GTGCAG 1 cut(s) 11
Bsh1285I CGRYCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 536, 591, 1193
BshVI ATCGAT 1 cut(s) 648
BsiEI CGRYCG 1 cut(s) 1135
BsiHKAI GWGCWC 2 cut(s) 218, 1070
BsiHKCI CYCGRG 1 cut(s) 186
BsiSI CCGG 4 cut(s) 116, 156, 521, 588
BslFI GGGAC 1 cut(s) 974
BslI CCNNNNNNNGG 2 cut(s) 679, 981
BsmAI GTCTC 2 cut(s) 183, 984
BsmBI CGTCTC 1 cut(s) 183
BsmFI GGGAC 1 cut(s) 974
BsmI GAATGC 2 cut(s) 104, 892
BsnI GGCC 3 cut(s) 536, 591, 1193
BsoBI CYCGRG 1 cut(s) 186
Bsp119I TTCGAA 1 cut(s) 868
Bsp1286I GDGCHC 2 cut(s) 218, 1070
Bsp143I GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
Bsp1720I GCTNAGC 2 cut(s) 212, 1004
Bsp19I CCATGG 1 cut(s) 289
BspACI CCGC 1 cut(s) 516
BspANI GGCC 3 cut(s) 536, 591, 1193
BspCNI CTCAG 5 cut(s) 204, 325, 840, 1017, 1033
BspDI ATCGAT 1 cut(s) 648
BspLI GGNNCC 1 cut(s) 120
BspPI GGATC 3 cut(s) 66, 176, 649
BspT104I TTCGAA 1 cut(s) 868
BsrFI RCCGGY 1 cut(s) 520
BsrI ACTGG 1 cut(s) 674
BssAI RCCGGY 1 cut(s) 520
BssECI CCNNGG 2 cut(s) 54, 289
BssMI GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
BssT1I CCWWGG 1 cut(s) 289
Bst4CI ACNGT 4 cut(s) 504, 575, 585, 1016
Bst6I CTCTTC 1 cut(s) 453
BstBAI YACGTR 1 cut(s) 138
BstBI TTCGAA 1 cut(s) 868
BstC8I GCNNGC 1 cut(s) 1191
BstDEI CTNAG 6 cut(s) 212, 333, 338, 827, 1004, 1041
BstDSI CCRYGG 2 cut(s) 54, 289
BstKTI GATC 9 cut(s) 61, 171, 237, 657, 895, 944, 1028, 1075, 1105
BstMAI GTCTC 2 cut(s) 183, 984
BstMBI GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
BstMCI CGRYCG 1 cut(s) 1135
BstMWI GCNNNNNNNGC 3 cut(s) 101, 412, 739
BstNSI RCATGY 1 cut(s) 665
BstSCI CCNGG 1 cut(s) 155
BstV1I GCAGC 5 cut(s) 79, 286, 452, 997, 1107
BstV2I GAAGAC 5 cut(s) 335, 489, 857, 920, 927
BstX2I RGATCY 2 cut(s) 168, 654
BstYI RGATCY 2 cut(s) 168, 654
Bsu15I ATCGAT 1 cut(s) 648
BsuI GTATCC 1 cut(s) 615
BsuRI GGCC 3 cut(s) 536, 591, 1193
BsuTUI ATCGAT 1 cut(s) 648
BtgI CCRYGG 2 cut(s) 54, 289
BtrI CACGTC 1 cut(s) 202
BtsIMutI CAGTG 3 cut(s) 330, 948, 1038
Cac8I GCNNGC 1 cut(s) 1191
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 3 cut(s) 118, 518, 972
ClaI ATCGAT 1 cut(s) 648
CpoI CGGWCCG 1 cut(s) 518
Csp6I GTAC 5 cut(s) 139, 319, 466, 496, 814
CspI CGGWCCG 1 cut(s) 518
CviAII CATG 6 cut(s) 290, 364, 662, 689, 722, 979
CviQI GTAC 5 cut(s) 139, 319, 466, 496, 814
DdeI CTNAG 6 cut(s) 212, 333, 338, 827, 1004, 1041
DpnI GATC 9 cut(s) 60, 170, 236, 656, 894, 943, 1027, 1074, 1104
DpnII GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
DriI GACNNNNNGTC 1 cut(s) 124
EaeI YGGCCR 1 cut(s) 534
Eam1104I CTCTTC 1 cut(s) 453
Eam1105I GACNNNNNGTC 1 cut(s) 124
EarI CTCTTC 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 289
Eco47I GGWCC 3 cut(s) 118, 518, 972
Eco57I CTGAAG 4 cut(s) 717, 932, 939, 1205
Eco88I CYCGRG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 289
Esp3I CGTCTC 1 cut(s) 183
FaeI CATG 6 cut(s) 293, 367, 665, 692, 725, 982
FaqI GGGAC 1 cut(s) 974
FatI CATG 6 cut(s) 289, 363, 661, 688, 721, 978
FauNDI CATATG 2 cut(s) 130, 424
Fnu4HI GCNGC 5 cut(s) 93, 300, 441, 986, 1096
Fsp4HI GCNGC 5 cut(s) 93, 300, 441, 986, 1096
FspBI CTAG 3 cut(s) 969, 999, 1119
GluI GCNGC 5 cut(s) 93, 300, 441, 986, 1096
HaeIII GGCC 3 cut(s) 536, 591, 1193
HapII CCGG 4 cut(s) 116, 156, 521, 588
Hin1II CATG 6 cut(s) 293, 367, 665, 692, 725, 982
HincII GTYRAC 1 cut(s) 199
HindII GTYRAC 1 cut(s) 199
HinfI GANTC 2 cut(s) 685, 902
HpaII CCGG 4 cut(s) 116, 156, 521, 588
HphI GGTGA 4 cut(s) 217, 694, 897, 1162
Hpy166II GTNNAC 1 cut(s) 199
Hpy188I TCNGA 7 cut(s) 175, 315, 579, 901, 912, 1111, 1185
Hpy188III TCNNGA 9 cut(s) 62, 188, 399, 613, 809, 829, 1029, 1059, 1080
Hpy8I GTNNAC 1 cut(s) 199
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 2 cut(s) 20, 602
HpyCH4III ACNGT 4 cut(s) 504, 575, 585, 1016
HpyCH4IV ACGT 4 cut(s) 137, 201, 486, 1173
HpyCH4V TGCA 6 cut(s) 28, 77, 95, 428, 747, 890
HpyF10VI GCNNNNNNNGC 3 cut(s) 101, 412, 739
HpyF3I CTNAG 6 cut(s) 212, 333, 338, 827, 1004, 1041
HpySE526I ACGT 4 cut(s) 137, 201, 486, 1173
Hsp92II CATG 6 cut(s) 293, 367, 665, 692, 725, 982
Kzo9I GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
LmnI GCTCC 2 cut(s) 739, 1092
Lsp1109I GCAGC 5 cut(s) 79, 286, 452, 997, 1107
LweI GCATC 1 cut(s) 832
MaeI CTAG 3 cut(s) 969, 999, 1119
MaeII ACGT 4 cut(s) 137, 201, 486, 1173
MaeIII GTNAC 3 cut(s) 791, 903, 1169
MalI GATC 9 cut(s) 60, 170, 236, 656, 894, 943, 1027, 1074, 1104
MboI GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
MflI RGATCY 2 cut(s) 168, 654
MhlI GDGCHC 2 cut(s) 218, 1070
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 3 cut(s) 34, 47, 871
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 1 cut(s) 911
MmeI TCCRAC 2 cut(s) 1089, 1141
Mox20I TGGCCA 1 cut(s) 536
MroXI GAANNNNTTC 2 cut(s) 9, 620
MscI TGGCCA 1 cut(s) 536
MslI CAYNNNNRTG 2 cut(s) 294, 351
Msp20I TGGCCA 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 733
MspI CCGG 4 cut(s) 116, 156, 521, 588
MspR9I CCNGG 1 cut(s) 157
Mva1269I GAATGC 2 cut(s) 104, 892
MwoI GCNNNNNNNGC 3 cut(s) 101, 412, 739
NciI CCSGG 1 cut(s) 157
NcoI CCATGG 1 cut(s) 289
NdeI CATATG 2 cut(s) 130, 424
NdeII GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
NlaIII CATG 6 cut(s) 293, 367, 665, 692, 725, 982
NlaIV GGNNCC 1 cut(s) 120
NmuCI GTSAC 1 cut(s) 903
NspI RCATGY 1 cut(s) 665
NspV TTCGAA 1 cut(s) 868
PaeR7I CTCGAG 1 cut(s) 186
PciI ACATGT 1 cut(s) 661
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 2 cut(s) 104, 892
PdmI GAANNNNTTC 2 cut(s) 9, 620
PfeI GAWTC 1 cut(s) 685
PkrI GCNGC 5 cut(s) 94, 301, 442, 987, 1097
PleI GAGTC 1 cut(s) 910
PpsI GAGTC 1 cut(s) 910
Ppu21I YACGTR 1 cut(s) 138
PscI ACATGT 1 cut(s) 661
PsiI TTATAA 1 cut(s) 639
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 3 cut(s) 118, 518, 972
PsuI RGATCY 2 cut(s) 168, 654
PvuII CAGCTG 1 cut(s) 733
RsaI GTAC 5 cut(s) 140, 320, 467, 497, 815
RsaNI GTAC 5 cut(s) 139, 319, 466, 496, 814
RseI CAYNNNNRTG 2 cut(s) 294, 351
Rsr2I CGGWCCG 1 cut(s) 518
RsrII CGGWCCG 1 cut(s) 518
SatI GCNGC 5 cut(s) 93, 300, 441, 986, 1096
Sau3AI GATC 9 cut(s) 58, 168, 234, 654, 892, 941, 1025, 1072, 1102
Sau96I GGNCC 3 cut(s) 118, 518, 972
ScaI AGTACT 1 cut(s) 815
SchI GAGTC 1 cut(s) 911
ScrFI CCNGG 1 cut(s) 157
SduI GDGCHC 2 cut(s) 218, 1070
SfaNI GCATC 1 cut(s) 832
Sfr274I CTCGAG 1 cut(s) 186
SfuI TTCGAA 1 cut(s) 868
SinI GGWCC 3 cut(s) 118, 518, 972
SlaI CTCGAG 1 cut(s) 186
SmiMI CAYNNNNRTG 2 cut(s) 294, 351
SmlI CTYRAG 5 cut(s) 186, 553, 773, 817, 955
SmoI CTYRAG 5 cut(s) 186, 553, 773, 817, 955
Sse9I AATT 3 cut(s) 34, 47, 871
SsiI CCGC 1 cut(s) 516
SspMI CTAG 3 cut(s) 969, 999, 1119
StyD4I CCNGG 1 cut(s) 155
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 4 cut(s) 504, 575, 585, 1016
TaiI ACGT 4 cut(s) 140, 204, 489, 1176
TaqI TCGA 7 cut(s) 144, 187, 595, 630, 648, 868, 1131
TaqII GACCGA 2 cut(s) 1065, 1149
TasI AATT 3 cut(s) 34, 47, 871
TatI WGTACW 1 cut(s) 813
TfiI GAWTC 1 cut(s) 685
TscAI CASTG 3 cut(s) 337, 955, 1045
TseFI GTSAC 1 cut(s) 903
TseI GCWGC 5 cut(s) 92, 299, 440, 985, 1095
Tsp45I GTSAC 1 cut(s) 903
TspDTI ATGAA 5 cut(s) 335, 677, 717, 774, 826
TspGWI ACGGA 3 cut(s) 71, 114, 513
TspRI CASTG 3 cut(s) 337, 955, 1045
VpaK11BI GGWCC 3 cut(s) 118, 518, 972
XapI RAATTY 2 cut(s) 47, 871
XceI RCATGY 1 cut(s) 665
XhoI CTCGAG 1 cut(s) 186
XmnI GAANNNNTTC 2 cut(s) 9, 620
XspI CTAG 3 cut(s) 969, 999, 1119
ZrmI AGTACT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.