Rh4BG362600

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0011268
Physical Location & Seq
Reverse (-)
52118651 .. 52120762
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG362600.1

Sequence Viewer

Length: 1140 bp
ATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTTGCATTTTTTCCCCCAGATCCTCCTTCCTATAACATATTCGTTGATGAACCGACTGGGAAAATGAGGATCTCTGATGTTCATCCCAGAGAAGATGTGGACGTGGTGAAGATCAACACCAAGAAAGGGAACGAGATTGTTGCCTTGTTTATCAAAAACCCATCTGCTTCACTCACAGTGCTTTACTCTCATGGAAATGCTGCTGATATTGGTCAGATGTACCACGTTTTCAGTGAGCTTAGTGTTTATCTGGGTGTTAATCTAATGGGGTATGACTATTCTGGTTATGGACAATCTACTGGAAAGCCAAGTGAGCAGGACACATATGCAGACATAGAGGCTGCATATAAATGCCTTGAAGACACCTATGGTGTAAAGGAGGAAGATATTATATTGTACGGACAGTCAGTTGGAAGTGGACCGACTTTGGATTTGGCTACTCATTTGCCTCACGTGAGAGCTGTGATTCTTCACAGTCCAATACTTTCTGGCCTTCGAGTAATGTACCCTGTTAAGAAAACATTCTGGTTTGACATTTACAAGAATATTGATAAAATGCCACTCGTCAATAGCCCGGTTTTGGTGATTCATGGAACTGAAGATGATGTGGTGGATATTTCCCATGGTAAGCAGCTGTGGGAGCTTAGCAAAGACAAGTATGAACCTTTGTGGCTTAAAGGTGGTAACCATTGTAATCTGGAACTCTATCCAGAGTACTTGAGGCATCTCAGGAAGTTCATATCTGCCATTGAGAAGTTACCACGACACCGGAATCTAACTGAGCTGAGTCCTCTCAAGACCGTAGACTACAATGACAAGGCAAGACCAAGCAGTGGGAAGAGGGGCAAGGCTGCTAGGTGGAGCACCGGGCAGCTGAGAGAAAAATCAAGGAGGAGCACAGAGAATGGAGAGAAATCATCATCAAGAAGCAGTACTTATAGTAGAGAGAAATCATCGAGAAAGCTAAGTGTGGATCGATCTGGGAAGGCAAGGAATAGCACAGACTCAGATAGAGCAAGAAACAGCTTCGACCGCTTGGGAGATATGGTGAGATCAGTTGGATTGTGCAATGTTGATTGTATGAAGCCGGCAGCTTTCGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

379

Amino Acids

42.43

Weight (kDa)

8.76

Isoelectric Point (pI)

51.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 175 1.5e-09 Serine aminopeptidase, S33
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 866
AccI GTMKAC 1 cut(s) 837
AciI CCGC 1 cut(s) 1066
AclWI GGATC 3 cut(s) 47, 110, 1014
AcuI CTGAAG 1 cut(s) 651
AcvI CACGTG 1 cut(s) 487
AfaI GTAC 5 cut(s) 254, 431, 539, 749, 967
AfiI CCNNNNNNNGG 3 cut(s) 159, 613, 866
AgsI TTSAA 1 cut(s) 392
AjiI CACGTC 1 cut(s) 136
AluBI AGCT 9 cut(s) 271, 494, 667, 676, 817, 907, 997, 1059, 1127
AluI AGCT 9 cut(s) 271, 494, 667, 676, 817, 907, 997, 1059, 1127
Alw21I GWGCWC 2 cut(s) 899, 932
AlwI GGATC 3 cut(s) 47, 110, 1014
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 7 cut(s) 26, 233, 374, 664, 884, 904, 1124
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
Asp700I GAANNNNTTC 1 cut(s) 554
AspS9I GGNCC 1 cut(s) 452
AsuC2I CCSGG 2 cut(s) 608, 901
AsuHPI GGTGA 3 cut(s) 151, 628, 1093
AvaII GGWCC 1 cut(s) 452
BbrPI CACGTG 1 cut(s) 487
BbsI GAAGAC 1 cut(s) 399
Bbv12I GWGCWC 2 cut(s) 899, 932
BbvI GCAGC 7 cut(s) 13, 220, 361, 676, 871, 916, 1136
BccI CCATC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BcnI CCSGG 2 cut(s) 608, 901
BfaI CTAG 1 cut(s) 888
BisI GCNGC 7 cut(s) 27, 234, 375, 665, 885, 905, 1125
BlpI GCTNAGC 1 cut(s) 677
BlsI GCNGC 7 cut(s) 28, 235, 376, 666, 886, 906, 1126
BmcAI AGTACT 2 cut(s) 749, 967
Bme1390I CCNGG 2 cut(s) 608, 901
Bme18I GGWCC 1 cut(s) 452
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 452
BmrFI CCNGG 2 cut(s) 608, 901
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 99
BpiI GAAGAC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 677
BpuEI CTTGAG 2 cut(s) 772, 812
BpuMI CCSGG 2 cut(s) 608, 901
Bsa29I ATCGAT 1 cut(s) 1009
BsaAI YACGTR 1 cut(s) 487
BsaBI GATNNNNATC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 655
BsaWI WCCGGW 1 cut(s) 801
BsaXI ACNNNNNCTCC 2 cut(s) 1065, 1095
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 613, 866
Bse118I RCCGGY 1 cut(s) 1120
Bse1I ACTGG 2 cut(s) 94, 337
Bse3DI GCAATG 1 cut(s) 1108
Bse8I GATNNNNATC 1 cut(s) 114
BseCI ATCGAT 1 cut(s) 1009
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 1 cut(s) 115
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 3 cut(s) 159, 613, 866
BseMI GCAATG 1 cut(s) 1108
BseMII CTCAG 5 cut(s) 775, 804, 809, 899, 1053
BseNI ACTGG 2 cut(s) 94, 337
BseRI GAGGAG 1 cut(s) 940
BseXI GCAGC 7 cut(s) 13, 220, 361, 676, 871, 916, 1136
Bsh1285I CGRYCG 1 cut(s) 1066
BshFI GGCC 1 cut(s) 525
BshVI ATCGAT 1 cut(s) 1009
BsiEI CGRYCG 1 cut(s) 1066
BsiHKAI GWGCWC 2 cut(s) 899, 932
BsiSI CCGG 4 cut(s) 608, 802, 900, 1121
BslI CCNNNNNNNGG 3 cut(s) 159, 613, 866
BsnI GGCC 1 cut(s) 525
Bsp1286I GDGCHC 2 cut(s) 899, 932
Bsp143I GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
Bsp1720I GCTNAGC 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 1 cut(s) 1066
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 5 cut(s) 774, 805, 810, 900, 1052
BspDI ATCGAT 1 cut(s) 1009
BspPI GGATC 3 cut(s) 47, 110, 1014
BsrDI GCAATG 1 cut(s) 1108
BsrFI RCCGGY 1 cut(s) 1120
BsrI ACTGG 2 cut(s) 94, 337
BssAI RCCGGY 1 cut(s) 1120
BssECI CCNNGG 1 cut(s) 655
BssMI GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
BssT1I CCWWGG 1 cut(s) 655
Bst4CI ACNGT 4 cut(s) 211, 438, 509, 835
Bst6I CTCTTC 1 cut(s) 866
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 1122
BstDEI CTNAG 8 cut(s) 272, 677, 761, 813, 818, 908, 998, 1039
BstDSI CCRYGG 1 cut(s) 655
BstEII GGTNACC 1 cut(s) 716
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 6 cut(s) 55, 105, 147, 1009, 1013, 1088
BstMBI GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
BstMCI CGRYCG 1 cut(s) 1066
BstMWI GCNNNNNNNGC 5 cut(s) 35, 346, 673, 1065, 1133
BstPI GGTNACC 1 cut(s) 716
BstSCI CCNGG 2 cut(s) 606, 899
BstV1I GCAGC 7 cut(s) 13, 220, 361, 676, 871, 916, 1136
BstV2I GAAGAC 1 cut(s) 399
BstX2I RGATCY 2 cut(s) 52, 102
BstYI RGATCY 2 cut(s) 52, 102
Bsu15I ATCGAT 1 cut(s) 1009
BsuRI GGCC 1 cut(s) 525
BsuTUI ATCGAT 1 cut(s) 1009
BtgI CCRYGG 1 cut(s) 655
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 871
BtsIMutI CAGTG 3 cut(s) 216, 271, 871
Cac8I GCNNGC 1 cut(s) 1122
Cfr10I RCCGGY 1 cut(s) 1120
Cfr13I GGNCC 1 cut(s) 452
ClaI ATCGAT 1 cut(s) 1009
Csp6I GTAC 5 cut(s) 253, 430, 538, 748, 966
CviAII CATG 3 cut(s) 224, 623, 656
CviQI GTAC 5 cut(s) 253, 430, 538, 748, 966
DdeI CTNAG 8 cut(s) 272, 677, 761, 813, 818, 908, 998, 1039
DpnI GATC 6 cut(s) 54, 104, 146, 1008, 1012, 1087
DpnII GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
Eam1104I CTCTTC 1 cut(s) 866
EarI CTCTTC 1 cut(s) 866
Eco130I CCWWGG 1 cut(s) 655
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 651
Eco72I CACGTG 1 cut(s) 487
Eco91I GGTNACC 1 cut(s) 716
EcoO65I GGTNACC 1 cut(s) 716
EcoT14I CCWWGG 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 3 cut(s) 227, 626, 659
FalI AAGNNNNNCTT 2 cut(s) 952, 984
FatI CATG 3 cut(s) 223, 622, 655
FauNDI CATATG 1 cut(s) 358
FblI GTMKAC 1 cut(s) 837
Fnu4HI GCNGC 7 cut(s) 27, 234, 375, 665, 885, 905, 1125
FokI GGATG 1 cut(s) 102
Fsp4HI GCNGC 7 cut(s) 27, 234, 375, 665, 885, 905, 1125
FspBI CTAG 1 cut(s) 888
GluI GCNGC 7 cut(s) 27, 234, 375, 665, 885, 905, 1125
HaeIII GGCC 1 cut(s) 525
HapII CCGG 4 cut(s) 608, 802, 900, 1121
Hin1II CATG 3 cut(s) 227, 626, 659
HinfI GANTC 5 cut(s) 499, 619, 805, 820, 1037
HpaII CCGG 4 cut(s) 608, 802, 900, 1121
HphI GGTGA 3 cut(s) 151, 628, 1093
Hpy166II GTNNAC 3 cut(s) 133, 452, 838
Hpy188I TCNGA 3 cut(s) 109, 249, 1042
Hpy188III TCNNGA 6 cut(s) 731, 743, 763, 829, 957, 990
Hpy8I GTNNAC 3 cut(s) 133, 452, 838
HpyAV CCTTC 3 cut(s) 69, 536, 1012
HpyCH4III ACNGT 4 cut(s) 211, 438, 509, 835
HpyCH4IV ACGT 3 cut(s) 135, 258, 486
HpyCH4V TGCA 6 cut(s) 11, 29, 38, 362, 377, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 346, 673, 1065, 1133
HpyF3I CTNAG 8 cut(s) 272, 677, 761, 813, 818, 908, 998, 1039
HpySE526I ACGT 3 cut(s) 135, 258, 486
Hsp92II CATG 3 cut(s) 227, 626, 659
KroI GCCGGC 1 cut(s) 1120
KroNI GCCGGC 1 cut(s) 1122
Kzo9I GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
LmnI GCTCC 3 cut(s) 673, 894, 927
Lsp1109I GCAGC 7 cut(s) 13, 220, 361, 676, 871, 916, 1136
LweI GCATC 1 cut(s) 766
MaeI CTAG 1 cut(s) 888
MaeII ACGT 3 cut(s) 135, 258, 486
MaeIII GTNAC 2 cut(s) 716, 789
MalI GATC 6 cut(s) 54, 104, 146, 1008, 1012, 1087
MboI GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
MboII GAAGA 7 cut(s) 137, 154, 404, 428, 494, 644, 883
MflI RGATCY 2 cut(s) 52, 102
MhlI GDGCHC 2 cut(s) 899, 932
MlyI GAGTC 2 cut(s) 829, 1031
MmeI TCCRAC 2 cut(s) 424, 1072
MroNI GCCGGC 1 cut(s) 1120
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 3 cut(s) 291, 546, 708
MslI CAYNNNNRTG 2 cut(s) 228, 382
MspA1I CMGCKG 2 cut(s) 667, 907
MspI CCGG 4 cut(s) 608, 802, 900, 1121
MspR9I CCNGG 2 cut(s) 608, 901
MwoI GCNNNNNNNGC 5 cut(s) 35, 346, 673, 1065, 1133
NaeI GCCGGC 1 cut(s) 1122
NciI CCSGG 2 cut(s) 608, 901
NcoI CCATGG 1 cut(s) 655
NdeI CATATG 1 cut(s) 358
NdeII GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
NgoMIV GCCGGC 1 cut(s) 1120
NlaIII CATG 3 cut(s) 227, 626, 659
PdiI GCCGGC 1 cut(s) 1122
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 3 cut(s) 499, 619, 805
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 7 cut(s) 28, 235, 376, 666, 886, 906, 1126
PleI GAGTC 2 cut(s) 828, 1031
PmaCI CACGTG 1 cut(s) 487
PmlI CACGTG 1 cut(s) 487
PpsI GAGTC 2 cut(s) 828, 1031
Ppu21I YACGTR 1 cut(s) 487
PspCI CACGTG 1 cut(s) 487
PspEI GGTNACC 1 cut(s) 716
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 2 cut(s) 52, 102
PvuII CAGCTG 2 cut(s) 667, 907
RsaI GTAC 5 cut(s) 254, 431, 539, 749, 967
RsaNI GTAC 5 cut(s) 253, 430, 538, 748, 966
RseI CAYNNNNRTG 2 cut(s) 228, 382
SaqAI TTAA 3 cut(s) 291, 546, 708
SatI GCNGC 7 cut(s) 27, 234, 375, 665, 885, 905, 1125
Sau3AI GATC 6 cut(s) 52, 102, 144, 1006, 1010, 1085
Sau96I GGNCC 1 cut(s) 452
ScaI AGTACT 2 cut(s) 749, 967
SchI GAGTC 2 cut(s) 829, 1031
ScrFI CCNGG 2 cut(s) 608, 901
SduI GDGCHC 2 cut(s) 899, 932
SfaNI GCATC 1 cut(s) 766
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 2 cut(s) 228, 382
SmlI CTYRAG 2 cut(s) 751, 827
SmoI CTYRAG 2 cut(s) 751, 827
SsiI CCGC 1 cut(s) 1066
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 888
StyD4I CCNGG 2 cut(s) 606, 899
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 4 cut(s) 211, 438, 509, 835
TaiI ACGT 3 cut(s) 138, 261, 489
TaqI TCGA 5 cut(s) 529, 989, 1009, 1062, 1131
TaqII GACCGA 1 cut(s) 469
TatI WGTACW 2 cut(s) 747, 965
TfiI GAWTC 3 cut(s) 499, 619, 805
Tru1I TTAA 3 cut(s) 291, 546, 708
Tru9I TTAA 3 cut(s) 291, 546, 708
TscAI CASTG 3 cut(s) 216, 271, 871
TseI GCWGC 7 cut(s) 26, 233, 374, 664, 884, 904, 1124
TspDTI ATGAA 6 cut(s) 96, 104, 611, 708, 760, 1130
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 3 cut(s) 216, 271, 871
Van91I CCANNNNNTGG 1 cut(s) 866
VpaK11BI GGWCC 1 cut(s) 452
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 837
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 1 cut(s) 888
ZrmI AGTACT 2 cut(s) 749, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.