RLG00000006466

Alpha beta hydrolase domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0011268
Physical Location & Seq
Forward (+)
7465320 .. 7467424
2105 bp
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UTR
Exon/CDS
Intron
RLM00000006466

Sequence Viewer

Length: 1137 bp
ATGGGGTCTGCAACATCGTCTATGGCTGCAAAGTTTGCATTTTTTCCCCCAGATCCTCCTTCATATAACATATTCGTTGATGAACCGACTGGGAAAATGAGGATCTCTGATGTTCATCCCAGAGAAGATGTGGACGTGATGAAGATCAACACCAAGAAAGGGAACGAGATTGTTGCCTTGTTTATCAAAAACCCATCTGCTTCACTCACAGTGCTTTACTCTCATGGAAATGCAGCTGATATTGGTCAGATGTACCACGTTTTCAGTGAGCTTAGTGTTTATCTGGGTGTTAATCTAATGGGGTATGACTATTCTGGTTATGGACAATCTACTGGAAAGCCAAGTGAGCAGGACACATATGCAGACATAGAGGCTGCATATAAATGCCTTGAAGATACCTATGGTGTAAAGGAGGAAGATATTATATTGTACGGACAGTCAGTTGGAAGTGGACCGACTTTGGATTTGGCTGCTCGTTTGCCTCACGTGAGAGCTGTGGTTCTTCACAGCCCAATACTTTCTGGCCTTCGAGTCATGTACCCTGTTAAGAAAACATTCTGGTTTGACATTTACAAGAATATTGATAAAATGCCACTCGTCAATAGCCCGGTTTTGGTGATTCATGGAACTGAAGATGATGTCGTGGATATTTCCCATGGTAAGCAGCTGTGGGAGCTTAGCAAAGACAAGTATGAACCTTTGTGGCTTAAAGGTGGGAACCATTGTAATCTGGAACTCTATCCAGAGTACTTGAGGCATCTCAGGAAGTTCATATCTGCCATTGAGAAGTTACCACGACACCGGAATCTAACCGAGCTGAGTCCTCTCAAGACCGTAGACGACAATGACAAGGCAAGACCAAGCAGTGGGAAGAGGGACAAGGCTAGGTGGAGCACCGGGCAGCTGAGAGAAAAATCAAGGAGGAGCACAGAGAATGGAGAGAAATCGTCATCAAGAAGCAGTACTTTTAGTAGAGAGAAATCATCGAGAAAGCTAAGTGTGGATCGATCTGGGAAGGCAAGGAATAGCACAGACTCGGATAGAGCAAGAAACAGCTTCGACCGCTTGGGAGATATGGTGAGATCAGTTGGATTGTGCAATGTTGATTGTATGAAGCCGGCAGCTTTCGAGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hydrolase_4

Protein Analysis

379

Amino Acids

42.36

Weight (kDa)

8.63

Isoelectric Point (pI)

51.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase_4 PF12146 69 - 175 1.1e-09 Serine aminopeptidase, S33
Abhydrolase_1 PF00561 71 - 171 3.8e-07 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011268)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G13610 AT1G13610
fragaria_vesca FvH4_4g29030
malus_domestica MD13G1055400.v1.1 MD16G1054200.v1.1
prunus_persica Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1 Prupe.1G295500_v2.0.a1
pyrus_communis pycom13g04900 pycom16g04720
rosa_chinensis RchiOBHm_Chr4g0437131
rosa_laevigata RLG00000006466
rosa_multiflora Rmu_ssc0000006.1_g000003
rosa_roxburghii Rroxscaffold_5G00378200
rosa_rugosa Rorug04G0298200
rosa_samantha Rh4AG353100 Rh4BG362600 Rh4CG376800 Rh4DG356600
rosa_wichuraiana Rw4G030930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 866
AccI GTMKAC 1 cut(s) 837
AciI CCGC 1 cut(s) 1063
AclWI GGATC 3 cut(s) 47, 110, 1011
AcuI CTGAAG 1 cut(s) 651
AcvI CACGTG 1 cut(s) 487
AfaI GTAC 5 cut(s) 254, 431, 539, 749, 964
AfiI CCNNNNNNNGG 3 cut(s) 159, 613, 866
AgsI TTSAA 1 cut(s) 392
AjiI CACGTC 1 cut(s) 136
Alw21I GWGCWC 2 cut(s) 896, 929
AlwI GGATC 3 cut(s) 47, 110, 1011
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 7 cut(s) 26, 233, 374, 470, 664, 901, 1121
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
Asp700I GAANNNNTTC 1 cut(s) 554
AspS9I GGNCC 1 cut(s) 452
AsuC2I CCSGG 2 cut(s) 608, 898
AsuHPI GGTGA 2 cut(s) 628, 1090
AvaII GGWCC 1 cut(s) 452
BbrPI CACGTG 1 cut(s) 487
Bbv12I GWGCWC 2 cut(s) 896, 929
BbvI GCAGC 7 cut(s) 13, 245, 361, 457, 676, 913, 1133
BccI CCATC 1 cut(s) 202
BcgI CGANNNNNNTGC 2 cut(s) 155, 189
BcnI CCSGG 2 cut(s) 608, 898
BfaI CTAG 1 cut(s) 885
BisI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
BlpI GCTNAGC 1 cut(s) 677
BlsI GCNGC 7 cut(s) 28, 235, 376, 472, 666, 903, 1123
BmcAI AGTACT 2 cut(s) 749, 964
Bme1390I CCNGG 2 cut(s) 608, 898
Bme18I GGWCC 1 cut(s) 452
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 1 cut(s) 452
BmiI GGNNCC 1 cut(s) 719
BmrFI CCNGG 2 cut(s) 608, 898
BmrI ACTGGG 1 cut(s) 99
BmsI GCATC 1 cut(s) 766
BmuI ACTGGG 1 cut(s) 99
Bpu1102I GCTNAGC 1 cut(s) 677
BpuEI CTTGAG 2 cut(s) 772, 812
BpuMI CCSGG 2 cut(s) 608, 898
Bsa29I ATCGAT 1 cut(s) 1006
BsaAI YACGTR 1 cut(s) 487
BsaBI GATNNNNATC 2 cut(s) 114, 143
BsaJI CCNNGG 1 cut(s) 655
BsaWI WCCGGW 1 cut(s) 801
BsaXI ACNNNNNCTCC 2 cut(s) 1062, 1092
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 613, 866
Bse118I RCCGGY 1 cut(s) 1117
Bse1I ACTGG 2 cut(s) 94, 337
Bse3DI GCAATG 1 cut(s) 1105
Bse8I GATNNNNATC 2 cut(s) 114, 143
BseCI ATCGAT 1 cut(s) 1006
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 1 cut(s) 115
BseJI GATNNNNATC 2 cut(s) 114, 143
BseLI CCNNNNNNNGG 3 cut(s) 159, 613, 866
BseMI GCAATG 1 cut(s) 1105
BseMII CTCAG 3 cut(s) 775, 809, 896
BseNI ACTGG 2 cut(s) 94, 337
BseRI GAGGAG 1 cut(s) 937
BseXI GCAGC 7 cut(s) 13, 245, 361, 457, 676, 913, 1133
Bsh1285I CGRYCG 1 cut(s) 1063
BshFI GGCC 1 cut(s) 525
BshVI ATCGAT 1 cut(s) 1006
BsiEI CGRYCG 1 cut(s) 1063
BsiHKAI GWGCWC 2 cut(s) 896, 929
BsiSI CCGG 4 cut(s) 608, 802, 897, 1118
BslFI GGGAC 1 cut(s) 890
BslI CCNNNNNNNGG 3 cut(s) 159, 613, 866
BsmFI GGGAC 1 cut(s) 890
BsnI GGCC 1 cut(s) 525
Bsp1286I GDGCHC 2 cut(s) 896, 929
Bsp143I GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
Bsp1720I GCTNAGC 1 cut(s) 677
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 1 cut(s) 1063
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 3 cut(s) 774, 810, 897
BspDI ATCGAT 1 cut(s) 1006
BspLI GGNNCC 1 cut(s) 719
BspPI GGATC 3 cut(s) 47, 110, 1011
BsrDI GCAATG 1 cut(s) 1105
BsrFI RCCGGY 1 cut(s) 1117
BsrI ACTGG 2 cut(s) 94, 337
BssAI RCCGGY 1 cut(s) 1117
BssECI CCNNGG 1 cut(s) 655
BssMI GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
BssT1I CCWWGG 1 cut(s) 655
Bst4CI ACNGT 3 cut(s) 211, 438, 835
Bst6I CTCTTC 1 cut(s) 866
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBAI YACGTR 1 cut(s) 487
BstC8I GCNNGC 1 cut(s) 1119
BstDEI CTNAG 6 cut(s) 272, 677, 761, 818, 905, 995
BstDSI CCRYGG 1 cut(s) 655
BstF5I GGATG 1 cut(s) 115
BstKTI GATC 6 cut(s) 55, 105, 147, 1006, 1010, 1085
BstMBI GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
BstMCI CGRYCG 1 cut(s) 1063
BstMWI GCNNNNNNNGC 5 cut(s) 35, 346, 673, 1062, 1130
BstSCI CCNGG 2 cut(s) 606, 896
BstV1I GCAGC 7 cut(s) 13, 245, 361, 457, 676, 913, 1133
BstX2I RGATCY 2 cut(s) 52, 102
BstYI RGATCY 2 cut(s) 52, 102
Bsu15I ATCGAT 1 cut(s) 1006
BsuRI GGCC 1 cut(s) 525
BsuTUI ATCGAT 1 cut(s) 1006
BtgI CCRYGG 1 cut(s) 655
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 1 cut(s) 115
BtsI GCAGTG 1 cut(s) 871
BtsIMutI CAGTG 3 cut(s) 216, 271, 871
Cac8I GCNNGC 1 cut(s) 1119
Cfr10I RCCGGY 1 cut(s) 1117
Cfr13I GGNCC 1 cut(s) 452
ClaI ATCGAT 1 cut(s) 1006
Csp6I GTAC 5 cut(s) 253, 430, 538, 748, 963
CviAII CATG 4 cut(s) 224, 535, 623, 656
CviQI GTAC 5 cut(s) 253, 430, 538, 748, 963
DdeI CTNAG 6 cut(s) 272, 677, 761, 818, 905, 995
DpnI GATC 6 cut(s) 54, 104, 146, 1005, 1009, 1084
DpnII GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
Eam1104I CTCTTC 1 cut(s) 866
EarI CTCTTC 1 cut(s) 866
Eco130I CCWWGG 1 cut(s) 655
Eco47I GGWCC 1 cut(s) 452
Eco57I CTGAAG 1 cut(s) 651
Eco72I CACGTG 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 655
FaeI CATG 4 cut(s) 227, 538, 626, 659
FalI AAGNNNNNCTT 2 cut(s) 949, 981
FaqI GGGAC 1 cut(s) 890
FatI CATG 4 cut(s) 223, 534, 622, 655
FauNDI CATATG 1 cut(s) 358
FblI GTMKAC 1 cut(s) 837
Fnu4HI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
FokI GGATG 1 cut(s) 102
Fsp4HI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
FspBI CTAG 1 cut(s) 885
GluI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
HaeIII GGCC 1 cut(s) 525
HapII CCGG 4 cut(s) 608, 802, 897, 1118
Hin1II CATG 4 cut(s) 227, 538, 626, 659
HinfI GANTC 5 cut(s) 531, 619, 805, 820, 1034
HpaII CCGG 4 cut(s) 608, 802, 897, 1118
HphI GGTGA 2 cut(s) 628, 1090
Hpy166II GTNNAC 3 cut(s) 133, 452, 838
Hpy188I TCNGA 3 cut(s) 109, 249, 1039
Hpy188III TCNNGA 6 cut(s) 731, 743, 763, 829, 954, 987
Hpy8I GTNNAC 3 cut(s) 133, 452, 838
HpyAV CCTTC 3 cut(s) 69, 536, 1009
HpyCH4III ACNGT 3 cut(s) 211, 438, 835
HpyCH4IV ACGT 3 cut(s) 135, 258, 486
HpyCH4V TGCA 7 cut(s) 11, 29, 38, 233, 362, 377, 1098
HpyF10VI GCNNNNNNNGC 5 cut(s) 35, 346, 673, 1062, 1130
HpyF3I CTNAG 6 cut(s) 272, 677, 761, 818, 905, 995
HpySE526I ACGT 3 cut(s) 135, 258, 486
Hsp92II CATG 4 cut(s) 227, 538, 626, 659
KroI GCCGGC 1 cut(s) 1117
KroNI GCCGGC 1 cut(s) 1119
Kzo9I GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
LmnI GCTCC 3 cut(s) 673, 891, 924
Lsp1109I GCAGC 7 cut(s) 13, 245, 361, 457, 676, 913, 1133
LweI GCATC 1 cut(s) 766
MaeI CTAG 1 cut(s) 885
MaeII ACGT 3 cut(s) 135, 258, 486
MaeIII GTNAC 1 cut(s) 789
MalI GATC 6 cut(s) 54, 104, 146, 1005, 1009, 1084
MboI GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
MboII GAAGA 7 cut(s) 137, 154, 404, 428, 494, 644, 883
MflI RGATCY 2 cut(s) 52, 102
MhlI GDGCHC 2 cut(s) 896, 929
MlyI GAGTC 3 cut(s) 540, 829, 1028
MmeI TCCRAC 2 cut(s) 424, 1069
MroNI GCCGGC 1 cut(s) 1117
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 3 cut(s) 291, 546, 708
MslI CAYNNNNRTG 2 cut(s) 228, 382
MspA1I CMGCKG 3 cut(s) 236, 667, 904
MspI CCGG 4 cut(s) 608, 802, 897, 1118
MspR9I CCNGG 2 cut(s) 608, 898
MwoI GCNNNNNNNGC 5 cut(s) 35, 346, 673, 1062, 1130
NaeI GCCGGC 1 cut(s) 1119
NciI CCSGG 2 cut(s) 608, 898
NcoI CCATGG 1 cut(s) 655
NdeI CATATG 1 cut(s) 358
NdeII GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
NgoMIV GCCGGC 1 cut(s) 1117
NlaIII CATG 4 cut(s) 227, 538, 626, 659
NlaIV GGNNCC 1 cut(s) 719
PdiI GCCGGC 1 cut(s) 1119
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 2 cut(s) 619, 805
PflMI CCANNNNNTGG 1 cut(s) 866
PkrI GCNGC 7 cut(s) 28, 235, 376, 472, 666, 903, 1123
PleI GAGTC 3 cut(s) 539, 828, 1028
PmaCI CACGTG 1 cut(s) 487
PmlI CACGTG 1 cut(s) 487
PpsI GAGTC 3 cut(s) 539, 828, 1028
Ppu21I YACGTR 1 cut(s) 487
PspCI CACGTG 1 cut(s) 487
PspN4I GGNNCC 1 cut(s) 719
PspPI GGNCC 1 cut(s) 452
PsuI RGATCY 2 cut(s) 52, 102
PvuII CAGCTG 3 cut(s) 236, 667, 904
RsaI GTAC 5 cut(s) 254, 431, 539, 749, 964
RsaNI GTAC 5 cut(s) 253, 430, 538, 748, 963
RseI CAYNNNNRTG 2 cut(s) 228, 382
SaqAI TTAA 3 cut(s) 291, 546, 708
SatI GCNGC 7 cut(s) 27, 234, 375, 471, 665, 902, 1122
Sau3AI GATC 6 cut(s) 52, 102, 144, 1003, 1007, 1082
Sau96I GGNCC 1 cut(s) 452
ScaI AGTACT 2 cut(s) 749, 964
SchI GAGTC 3 cut(s) 540, 829, 1028
ScrFI CCNGG 2 cut(s) 608, 898
SduI GDGCHC 2 cut(s) 896, 929
SfaNI GCATC 1 cut(s) 766
SinI GGWCC 1 cut(s) 452
SmiMI CAYNNNNRTG 2 cut(s) 228, 382
SmlI CTYRAG 2 cut(s) 751, 827
SmoI CTYRAG 2 cut(s) 751, 827
SsiI CCGC 1 cut(s) 1063
SspI AATATT 1 cut(s) 580
SspMI CTAG 1 cut(s) 885
StyD4I CCNGG 2 cut(s) 606, 896
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 3 cut(s) 211, 438, 835
TaiI ACGT 3 cut(s) 138, 261, 489
TaqI TCGA 5 cut(s) 529, 986, 1006, 1059, 1128
TaqII GACCGA 1 cut(s) 469
TatI WGTACW 2 cut(s) 747, 962
TfiI GAWTC 2 cut(s) 619, 805
Tru1I TTAA 3 cut(s) 291, 546, 708
Tru9I TTAA 3 cut(s) 291, 546, 708
TscAI CASTG 3 cut(s) 216, 271, 871
TseI GCWGC 7 cut(s) 26, 233, 374, 470, 664, 901, 1121
TspDTI ATGAA 8 cut(s) 51, 96, 104, 155, 611, 708, 760, 1127
TspGWI ACGGA 1 cut(s) 447
TspRI CASTG 3 cut(s) 216, 271, 871
Van91I CCANNNNNTGG 1 cut(s) 866
VpaK11BI GGWCC 1 cut(s) 452
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
XmiI GTMKAC 1 cut(s) 837
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 1 cut(s) 885
ZrmI AGTACT 2 cut(s) 749, 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.