FvH4_4g30040

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018028
Physical Location & Seq
Reverse (-)
29875269 .. 29876664
1396 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30040.t1

Sequence Viewer

Length: 1353 bp
ATGTGTTCAAATACGTCTAACAAGAAGCTGCGTCATATAAACTGCCCAGATTGGGCAAGTCTTCACGGATCGCTTGTTCATCTGTTGCTAGACAAGTTGTTGGAACCCATTGACCATGTTCGTTTTGCTGCAGTTTGCAAGGAGTGGCGTGCAGTTTCAAAAGATTACAATGAAGCAAAGCAGCGCTGGTGTAAAAAGAGGCTACTTCCGATGCTCTTGATCTCATCTGCTGAATCCAAAGAAAAGCTCGAACTATACAGCATTGCCGAAAAAAAAGTCTATAGCAACATCAAGTTACCATTGCCTTGTAGGAAGTCTAGGAGGTGTTGGCTCCAACCACGACAGCGGTGGTTGGTTGATCGTAGTAGATGTAGTAGAAGAAAAGAAGTTCCCCCCATTCATCTTCCTCGTTTGCAATTCAATCATGGATACAAATATCCATCTGGAAAAGCTCAGCAGCTCACGAGCTCTAAGGTTATACTATCCGGTGATCCCACATTGAATCCGGACAGTTTCTTGGTTTTAGCAATCAATGGACATAAAAATGGACACAATGTGTATTCCATTGAGGGACTAAATAGTGATTGGGTTTATTGGAAGTTTGCATATGGTTGTACTGATGCTATAGTTTACAAAAGCCAGGTCTACTCGATTACTGCTCAGAGATGCCTTAATTTGTTAGATGTTGATAAGGCAATAACAAACAGATTCCTTCCTCAAACGTATAGTCCATCAGAAAAGTTCTATCTTGTAGAATCAATACAGGGATACATGTTGCTTGTTCAAAGAGATATGGAATCGAAGAAGAATGTGAACAATGATTTGCATCAGAAACTTTGGACCAACAGCTTCGTTGTTCACAAGATTGGGTTCAAAAAGAGAGACGTAAAGCTGGACAGAGGAAAGCTAGTCTTTGATGTTGAAGAGCCGCCGTTGTATACTGGTGATGGAGATCGTTCACAGTATTTTAAGCTATTTGAGGTAGAAAGCATTAGAGATGAAGTTTTGTTTGTAGGTGACAACTATTCGTTCTCGGTTTTGGCTTCGGACTATCCAGGGTGTCAACCCAATTCCATATACTACACAGATGATTCCATGGAAACTCCATTGCATGAAGGTTATAGTACTTATGACTGCAGCGATGGTAGAGGTCGTAGTTTCGGACGCCGTGGAAGGGGAGGATACAAAGGACCTCAGGCCGATCTACAGCTCGTAGGGGACGCTCCTTATGACATGGGGATCTTCAACCTAGCGGATAAAAGCATCACACCACTTTGTTCTTCTCTAAGTCATTCCAAGACGAATGTCCGACCACCGTTTTGGACAATCCCACCATTTAGCGGACTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

451

Amino Acids

51.65

Weight (kDa)

9.11

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 85 - 371 9.3e-29 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018028)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30040 FvH4_4g30041 FvH4_4g30110
rosa_chinensis RchiOBHm_Chr4g0438341
rosa_laevigata RLG00000006369
rosa_multiflora Rmu_sc0002327.1_g000028 Rmu_sc0025676.1_g000002
rosa_rugosa Rorug04G0309100
rosa_samantha Rh4AG360200
rosa_wichuraiana Rw0G006480 Rw0G016430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 645, 938
AccIII TCCGGA 1 cut(s) 505
AciI CCGC 4 cut(s) 346, 929, 1253, 1341
AclWI GGATC 3 cut(s) 76, 485, 1247
AcyI GRCGYC 1 cut(s) 1165
AdeI CACNNNGTG 1 cut(s) 556
AfaI GTAC 2 cut(s) 616, 1126
AfeI AGCGCT 1 cut(s) 185
AfiI CCNNNNNNNGG 3 cut(s) 52, 1174, 1340
AflIII ACRYGT 1 cut(s) 771
AgsI TTSAA 8 cut(s) 9, 159, 421, 502, 785, 874, 923, 1246
AjnI CCWGG 2 cut(s) 639, 1054
AloI GAACNNNNNNTCC 2 cut(s) 60, 92
Alw21I GWGCWC 1 cut(s) 470
Alw26I GTCTC 1 cut(s) 876
AlwI GGATC 3 cut(s) 76, 485, 1247
Aor13HI TCCGGA 1 cut(s) 505
Aor51HI AGCGCT 1 cut(s) 185
AoxI GGCC 1 cut(s) 1197
ApeKI GCWGC 5 cut(s) 28, 128, 181, 457, 1137
ArsI GACNNNNNNTTYG 8 cut(s) 261, 293, 627, 659, 712, 744, 1302, 1334
AspLEI GCGC 1 cut(s) 186
AspS9I GGNCC 2 cut(s) 840, 1190
AsuHPI GGTGA 3 cut(s) 500, 956, 1028
AvaII GGWCC 2 cut(s) 840, 1190
AxyI CCTNAGG 1 cut(s) 1194
BanII GRGCYC 1 cut(s) 470
BauI CACGAG 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 53
Bbv12I GWGCWC 1 cut(s) 470
BbvI GCAGC 5 cut(s) 15, 115, 193, 469, 1149
BccI CCATC 4 cut(s) 448, 739, 941, 1136
BceAI ACGGC 2 cut(s) 916, 1152
BciT130I CCWGG 2 cut(s) 641, 1056
BciVI GTATCC 3 cut(s) 422, 761, 1175
BcoDI GTCTC 1 cut(s) 876
BfaI CTAG 4 cut(s) 89, 318, 908, 1250
BfmI CTRYAG 5 cut(s) 129, 280, 624, 1135, 1205
BfoI RGCGCY 1 cut(s) 187
BfuI GTATCC 3 cut(s) 422, 761, 1175
BisI GCNGC 6 cut(s) 29, 129, 182, 458, 929, 1138
BlpI GCTNAGC 1 cut(s) 453
BlsI GCNGC 6 cut(s) 30, 130, 183, 459, 930, 1139
BmcAI AGTACT 1 cut(s) 1126
Bme1390I CCNGG 2 cut(s) 641, 1056
Bme18I GGWCC 2 cut(s) 840, 1190
BmgT120I GGNCC 2 cut(s) 840, 1190
BmiI GGNNCC 2 cut(s) 105, 332
BmrFI CCNGG 2 cut(s) 641, 1056
BmsI GCATC 5 cut(s) 201, 610, 656, 835, 1272
BoxI GACNNNNGTC 1 cut(s) 1304
BpiI GAAGAC 1 cut(s) 53
Bpu1102I GCTNAGC 1 cut(s) 453
BsaBI GATNNNNATC 2 cut(s) 825, 951
BsaHI GRCGYC 1 cut(s) 1165
BsaJI CCNNGG 3 cut(s) 1055, 1095, 1168
BsaWI WCCGGW 2 cut(s) 485, 505
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 1174, 1340
Bse1I ACTGG 1 cut(s) 946
Bse21I CCTNAGG 1 cut(s) 1194
Bse3DI GCAATG 3 cut(s) 261, 299, 1106
Bse8I GATNNNNATC 2 cut(s) 825, 951
BseAI TCCGGA 1 cut(s) 505
BseBI CCWGG 2 cut(s) 641, 1056
BseDI CCNNGG 3 cut(s) 1055, 1095, 1168
BseJI GATNNNNATC 2 cut(s) 825, 951
BseLI CCNNNNNNNGG 3 cut(s) 52, 1174, 1340
BseMI GCAATG 3 cut(s) 261, 299, 1106
BseMII CTCAG 3 cut(s) 467, 674, 1208
BseNI ACTGG 1 cut(s) 946
BseXI GCAGC 5 cut(s) 15, 115, 193, 469, 1149
BsgI GTGCAG 1 cut(s) 171
BshFI GGCC 1 cut(s) 1199
BsiHKAI GWGCWC 1 cut(s) 470
BsiSI CCGG 2 cut(s) 486, 506
BslFI GGGAC 2 cut(s) 585, 1232
BslI CCNNNNNNNGG 3 cut(s) 52, 1174, 1340
BsmAI GTCTC 1 cut(s) 876
BsmBI CGTCTC 1 cut(s) 876
BsmFI GGGAC 2 cut(s) 585, 1232
BsnI GGCC 1 cut(s) 1199
Bsp1286I GDGCHC 1 cut(s) 470
Bsp13I TCCGGA 1 cut(s) 505
Bsp143I GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
Bsp1720I GCTNAGC 1 cut(s) 453
Bsp19I CCATGG 1 cut(s) 1095
BspACI CCGC 4 cut(s) 346, 929, 1253, 1341
BspANI GGCC 1 cut(s) 1199
BspCNI CTCAG 3 cut(s) 466, 673, 1207
BspEI TCCGGA 1 cut(s) 505
BspLI GGNNCC 2 cut(s) 105, 332
BspMAI CTGCAG 2 cut(s) 133, 1139
BspPI GGATC 3 cut(s) 76, 485, 1247
BspQI GCTCTTC 1 cut(s) 918
BsrDI GCAATG 3 cut(s) 261, 299, 1106
BsrI ACTGG 1 cut(s) 946
BssECI CCNNGG 3 cut(s) 1055, 1095, 1168
BssMI GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
BssNAI GTATAC 1 cut(s) 939
BssNI GRCGYC 1 cut(s) 1165
BssSI CACGAG 1 cut(s) 463
BssT1I CCWWGG 1 cut(s) 1095
Bst1107I GTATAC 1 cut(s) 939
Bst2BI CACGAG 1 cut(s) 463
Bst2UI CCWGG 2 cut(s) 641, 1056
Bst4CI ACNGT 3 cut(s) 512, 963, 1317
Bst6I CTCTTC 1 cut(s) 918
BstACI GRCGYC 1 cut(s) 1165
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 5 cut(s) 453, 471, 660, 1194, 1286
BstDSI CCRYGG 2 cut(s) 1095, 1168
BstH2I RGCGCY 1 cut(s) 187
BstHHI GCGC 1 cut(s) 186
BstKTI GATC 7 cut(s) 71, 222, 361, 493, 955, 1204, 1242
BstMAI GTCTC 1 cut(s) 876
BstMBI GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
BstNI CCWGG 2 cut(s) 641, 1056
BstNSI RCATGY 1 cut(s) 775
BstPAI GACNNNNGTC 1 cut(s) 1304
BstSCI CCNGG 2 cut(s) 639, 1054
BstSFI CTRYAG 5 cut(s) 129, 280, 624, 1135, 1205
BstV1I GCAGC 5 cut(s) 15, 115, 193, 469, 1149
BstV2I GAAGAC 1 cut(s) 53
BstX2I RGATCY 1 cut(s) 1239
BstXI CCANNNNNNTGG 1 cut(s) 1320
BstYI RGATCY 1 cut(s) 1239
BstZ17I GTATAC 1 cut(s) 939
Bsu36I CCTNAGG 1 cut(s) 1194
BsuI GTATCC 3 cut(s) 422, 761, 1175
BsuRI GGCC 1 cut(s) 1199
BtgI CCRYGG 2 cut(s) 1095, 1168
BtgZI GCGATG 1 cut(s) 1155
Cac8I GCNNGC 1 cut(s) 150
CfoI GCGC 1 cut(s) 186
Cfr13I GGNCC 2 cut(s) 840, 1190
CseI GACGC 3 cut(s) 20, 1173, 1229
Csp6I GTAC 2 cut(s) 615, 1125
CviAII CATG 6 cut(s) 116, 425, 772, 1096, 1112, 1234
CviQI GTAC 2 cut(s) 615, 1125
DdeI CTNAG 5 cut(s) 453, 471, 660, 1194, 1286
DpnI GATC 7 cut(s) 70, 221, 360, 492, 954, 1203, 1241
DpnII GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
DraIII CACNNNGTG 1 cut(s) 556
Eam1104I CTCTTC 1 cut(s) 918
EarI CTCTTC 1 cut(s) 918
Ecl136II GAGCTC 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 1095
Eco24I GRGCYC 1 cut(s) 470
Eco47I GGWCC 2 cut(s) 840, 1190
Eco47III AGCGCT 1 cut(s) 185
Eco53kI GAGCTC 1 cut(s) 468
Eco81I CCTNAGG 1 cut(s) 1194
EcoICRI GAGCTC 1 cut(s) 468
EcoO109I RGGNCCY 1 cut(s) 1190
EcoRII CCWGG 2 cut(s) 639, 1054
EcoT14I CCWWGG 1 cut(s) 1095
EcoT38I GRGCYC 1 cut(s) 470
ErhI CCWWGG 1 cut(s) 1095
Esp3I CGTCTC 1 cut(s) 876
FaeI CATG 6 cut(s) 119, 428, 775, 1099, 1115, 1237
FalI AAGNNNNNCTT 2 cut(s) 896, 928
FaqI GGGAC 2 cut(s) 585, 1232
FatI CATG 6 cut(s) 115, 424, 771, 1095, 1111, 1233
FauNDI CATATG 1 cut(s) 607
FblI GTMKAC 2 cut(s) 645, 938
Fnu4HI GCNGC 6 cut(s) 29, 129, 182, 458, 929, 1138
FriOI GRGCYC 1 cut(s) 470
Fsp4HI GCNGC 6 cut(s) 29, 129, 182, 458, 929, 1138
FspBI CTAG 4 cut(s) 89, 318, 908, 1250
GlaI GCGC 1 cut(s) 185
GluI GCNGC 6 cut(s) 29, 129, 182, 458, 929, 1138
HaeII RGCGCY 1 cut(s) 187
HaeIII GGCC 1 cut(s) 1199
HapII CCGG 2 cut(s) 486, 506
HgaI GACGC 3 cut(s) 20, 1173, 1229
HhaI GCGC 1 cut(s) 186
Hin1I GRCGYC 1 cut(s) 1165
Hin1II CATG 6 cut(s) 119, 428, 775, 1099, 1115, 1237
Hin6I GCGC 1 cut(s) 184
HinP1I GCGC 1 cut(s) 184
HincII GTYRAC 1 cut(s) 1064
HindII GTYRAC 1 cut(s) 1064
HinfI GANTC 7 cut(s) 233, 502, 708, 755, 797, 1091, 1344
HpaII CCGG 2 cut(s) 486, 506
HphI GGTGA 3 cut(s) 500, 956, 1028
Hpy166II GTNNAC 7 cut(s) 631, 646, 814, 859, 939, 959, 1064
Hpy188I TCNGA 7 cut(s) 210, 663, 736, 831, 1048, 1163, 1310
Hpy188III TCNNGA 4 cut(s) 217, 444, 463, 506
Hpy8I GTNNAC 7 cut(s) 631, 646, 814, 859, 939, 959, 1064
HpyAV CCTTC 3 cut(s) 722, 1109, 1167
HpyCH4III ACNGT 3 cut(s) 512, 963, 1317
HpyCH4IV ACGT 3 cut(s) 14, 722, 885
HpyCH4V TGCA 8 cut(s) 131, 138, 152, 415, 605, 826, 1111, 1137
HpyF3I CTNAG 5 cut(s) 453, 471, 660, 1194, 1286
HpySE526I ACGT 3 cut(s) 14, 722, 885
Hsp92I GRCGYC 1 cut(s) 1165
Hsp92II CATG 6 cut(s) 119, 428, 775, 1099, 1115, 1237
HspAI GCGC 1 cut(s) 184
Kpn2I TCCGGA 1 cut(s) 505
Kzo9I GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
LguI GCTCTTC 1 cut(s) 918
LmnI GCTCC 2 cut(s) 336, 1228
Lsp1109I GCAGC 5 cut(s) 15, 115, 193, 469, 1149
LweI GCATC 5 cut(s) 201, 610, 656, 835, 1272
MaeI CTAG 4 cut(s) 89, 318, 908, 1250
MaeII ACGT 3 cut(s) 14, 722, 885
MaeIII GTNAC 2 cut(s) 294, 1016
MalI GATC 7 cut(s) 70, 221, 360, 492, 954, 1203, 1241
MboI GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
MboII GAAGA 8 cut(s) 53, 390, 395, 814, 817, 935, 1234, 1272
MflI RGATCY 1 cut(s) 1239
MhlI GDGCHC 1 cut(s) 470
MluCI AATT 3 cut(s) 416, 673, 1069
MlyI GAGTC 1 cut(s) 1338
MmeI TCCRAC 3 cut(s) 81, 358, 1333
MroI TCCGGA 1 cut(s) 505
MseI TTAA 2 cut(s) 672, 969
MslI CAYNNNNRTG 1 cut(s) 543
MspA1I CMGCKG 1 cut(s) 346
MspI CCGG 2 cut(s) 486, 506
MspR9I CCNGG 2 cut(s) 641, 1056
MvaI CCWGG 2 cut(s) 641, 1056
NcoI CCATGG 1 cut(s) 1095
NdeI CATATG 1 cut(s) 607
NdeII GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
NlaIII CATG 6 cut(s) 119, 428, 775, 1099, 1115, 1237
NlaIV GGNNCC 2 cut(s) 105, 332
NmuCI GTSAC 1 cut(s) 1016
NspI RCATGY 1 cut(s) 775
PciI ACATGT 1 cut(s) 771
PciSI GCTCTTC 1 cut(s) 918
PfeI GAWTC 6 cut(s) 233, 502, 708, 755, 797, 1091
PkrI GCNGC 6 cut(s) 30, 130, 183, 459, 930, 1139
PleI GAGTC 1 cut(s) 1338
PpsI GAGTC 1 cut(s) 1338
PpuMI RGGWCCY 1 cut(s) 1190
PscI ACATGT 1 cut(s) 771
PshAI GACNNNNGTC 1 cut(s) 1304
Psp124BI GAGCTC 1 cut(s) 470
Psp5II RGGWCCY 1 cut(s) 1190
Psp6I CCWGG 2 cut(s) 639, 1054
PspGI CCWGG 2 cut(s) 639, 1054
PspN4I GGNNCC 2 cut(s) 105, 332
PspPI GGNCC 2 cut(s) 840, 1190
PspPPI RGGWCCY 1 cut(s) 1190
PstI CTGCAG 2 cut(s) 133, 1139
PsuI RGATCY 1 cut(s) 1239
RsaI GTAC 2 cut(s) 616, 1126
RsaNI GTAC 2 cut(s) 615, 1125
RseI CAYNNNNRTG 1 cut(s) 543
SacI GAGCTC 1 cut(s) 470
SapI GCTCTTC 1 cut(s) 918
SaqAI TTAA 2 cut(s) 672, 969
SatI GCNGC 6 cut(s) 29, 129, 182, 458, 929, 1138
Sau3AI GATC 7 cut(s) 68, 219, 358, 490, 952, 1201, 1239
Sau96I GGNCC 2 cut(s) 840, 1190
ScaI AGTACT 1 cut(s) 1126
SchI GAGTC 1 cut(s) 1338
ScrFI CCNGG 2 cut(s) 641, 1056
SduI GDGCHC 1 cut(s) 470
SfaNI GCATC 5 cut(s) 201, 610, 656, 835, 1272
SfcI CTRYAG 5 cut(s) 129, 280, 624, 1135, 1205
SinI GGWCC 2 cut(s) 840, 1190
SmiMI CAYNNNNRTG 1 cut(s) 543
Sse9I AATT 3 cut(s) 416, 673, 1069
SsiI CCGC 4 cut(s) 346, 929, 1253, 1341
SspMI CTAG 4 cut(s) 89, 318, 908, 1250
SstI GAGCTC 1 cut(s) 470
StyD4I CCNGG 2 cut(s) 639, 1054
StyI CCWWGG 1 cut(s) 1095
TaaI ACNGT 3 cut(s) 512, 963, 1317
TaiI ACGT 3 cut(s) 17, 725, 888
TaqI TCGA 3 cut(s) 249, 650, 800
TasI AATT 3 cut(s) 416, 673, 1069
TatI WGTACW 2 cut(s) 614, 1124
TauI GCSGC 1 cut(s) 931
TfiI GAWTC 6 cut(s) 233, 502, 708, 755, 797, 1091
Tru1I TTAA 2 cut(s) 672, 969
Tru9I TTAA 2 cut(s) 672, 969
TseFI GTSAC 1 cut(s) 1016
TseI GCWGC 5 cut(s) 28, 128, 181, 457, 1137
Tsp45I GTSAC 1 cut(s) 1016
TspDTI ATGAA 5 cut(s) 68, 186, 389, 1014, 1128
TspGWI ACGGA 1 cut(s) 81
VpaK11BI GGWCC 2 cut(s) 840, 1190
XceI RCATGY 1 cut(s) 775
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmiI GTMKAC 2 cut(s) 645, 938
XspI CTAG 4 cut(s) 89, 318, 908, 1250
ZrmI AGTACT 1 cut(s) 1126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.