Rw0G006480

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00245
OG0018028
Physical Location & Seq
Reverse (-)
186226 .. 187465
1240 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G006480.1

Sequence Viewer

Length: 1095 bp
ATGTGTTCAAAGACGTCTAACGAGAAGCTCTGTCGTATAGACTGCCCAGATTGGGCAAGTCTTCACAGAGCCTCTGTCTATTTGTTTCTGGACAAGTTTTTGGAACCCATTGATCTTGTTCATTTTGCTGCAGTTTGCAAGGAGTGGCGTGCAGTTTCAGAAGATTATAATGAAGCAAAACAGCGGTGGTGTAAAAAAGTCTACAGCAATATTGAGTCACCATTGCCTTGTAGTAATTCTAGGAGGTGTTTTGGCTCCAACCACGACAGTGGTGGTTGGTTGACCATAGTAGATCTAGTAGAGCGTGAGTTGCCATATTTAGATATAACTCTCACGAACCCATTCAGAAAAGAAGTGGCACCCATTAACCTTCCTCGTTTGCAATTCAAATATTTATCTCAAGAAGGACGTTGGCAGCTCCCGAAGGTTATATTATCCGGTGATCCCACATTGAACCCGGACAGTTTTGTGGTTTTAACAATCAATAGTCAATTTCAAAATGGACACCAGGTGTCTTCCATTAAGGGACTAAATAGTGATTGGGTTCATTGGGACTTTGCTTATAACGTTACTGATGCCATAGTTTATAGAAGCCAGATCTACTTGGTTACTGTTGATAGATGGCTCGCCTTGTTGGATGTTGACCAGGCAATAACAAACATATTCCTTCCGCCTCAACAAATTCTTCCGCCAGCTAAGGTATTCATGGAACTAAATAAGAATGTGAACGGTTTGCATCAGAAATTTTGGACCAATAGCTTCGTTATTCACAAGGTGGTGTTCATCAAGAGAGACAAAGAGCTGGACGGAGATATAAAATACTTAACGTTTACAGAGGGGACGATGTCTGTAGGTGATCGAGATCGTTCACAGTCGTTCGAGCAATTTGAGGTGACAACTATTCGTTCTCGGTTTTGGCTTCGGACTATCCTGGGTGCATGTCAACCCAATTCCATATACTACACAGATGATTCCATGGAAATTGAAGCTGGAGAAGTACCGTATGACATGGGTGTCTTCAATCACACCACTTTGTTCTCTGAATCATTCGGAGAGGAATGTTTAACCACCGCTTTGGACAATCCCACCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

364

Amino Acids

41.98

Weight (kDa)

5.35

Isoelectric Point (pI)

45.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 66 - 341 4.5e-16 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018028)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g30040 FvH4_4g30041 FvH4_4g30110
rosa_chinensis RchiOBHm_Chr4g0438341
rosa_laevigata RLG00000006369
rosa_multiflora Rmu_sc0002327.1_g000028 Rmu_sc0025676.1_g000002
rosa_rugosa Rorug04G0309100
rosa_samantha Rh4AG360200
rosa_wichuraiana Rw0G006480 Rw0G016430

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 168, 564
AasI GACNNNNNNGTC 1 cut(s) 1013
AatII GACGTC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 201
AciI CCGC 4 cut(s) 184, 671, 689, 1071
AclI AACGTT 2 cut(s) 567, 827
AclWI GGATC 1 cut(s) 437
AcsI RAATTY 2 cut(s) 681, 743
AcyI GRCGYC 1 cut(s) 14
AdeI CACNNNGTG 2 cut(s) 511, 775
AfaI GTAC 1 cut(s) 999
AfiI CCNNNNNNNGG 1 cut(s) 52
AgsI TTSAA 6 cut(s) 9, 388, 454, 497, 986, 1021
AjnI CCWGG 3 cut(s) 507, 645, 930
AleI CACNNNNGTG 1 cut(s) 267
AluBI AGCT 6 cut(s) 28, 418, 695, 759, 802, 989
AluI AGCT 6 cut(s) 28, 418, 695, 759, 802, 989
Alw26I GTCTC 1 cut(s) 786
AlwI GGATC 1 cut(s) 437
ApeKI GCWGC 2 cut(s) 128, 415
ApoI RAATTY 2 cut(s) 681, 743
Asp700I GAANNNNTTC 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 750
AsuC2I CCSGG 1 cut(s) 458
AsuHPI GGTGA 4 cut(s) 210, 452, 866, 904
AvaII GGWCC 1 cut(s) 750
BanI GGYRCC 1 cut(s) 358
BbsI GAAGAC 3 cut(s) 53, 507, 1009
BbvI GCAGC 2 cut(s) 115, 427
BccI CCATC 1 cut(s) 615
BciT130I CCWGG 3 cut(s) 509, 647, 932
BcnI CCSGG 1 cut(s) 458
BcoDI GTCTC 1 cut(s) 786
BfaI CTAG 2 cut(s) 240, 296
BfmI CTRYAG 3 cut(s) 129, 202, 849
BglII AGATCT 2 cut(s) 292, 597
BisI GCNGC 2 cut(s) 129, 416
BlsI GCNGC 2 cut(s) 130, 417
Bme1390I CCNGG 4 cut(s) 458, 509, 647, 932
Bme18I GGWCC 1 cut(s) 750
BmgT120I GGNCC 1 cut(s) 750
BmiI GGNNCC 3 cut(s) 105, 256, 360
BmrFI CCNGG 4 cut(s) 458, 509, 647, 932
BmsI GCATC 2 cut(s) 565, 745
BpiI GAAGAC 3 cut(s) 53, 507, 1009
BpmI CTGGAG 1 cut(s) 1011
Bpu10I CCTNAGC 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 384
BpuMI CCSGG 1 cut(s) 458
BsaBI GATNNNNATC 1 cut(s) 861
BsaHI GRCGYC 1 cut(s) 14
BsaJI CCNNGG 2 cut(s) 931, 975
BsaWI WCCGGW 1 cut(s) 437
Bsc4I CCNNNNNNNGG 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 221
Bse8I GATNNNNATC 1 cut(s) 861
BseBI CCWGG 3 cut(s) 509, 647, 932
BseDI CCNNGG 2 cut(s) 931, 975
BseGI GGATG 1 cut(s) 643
BseJI GATNNNNATC 1 cut(s) 861
BseLI CCNNNNNNNGG 1 cut(s) 52
BseMI GCAATG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 115, 427
BsgI GTGCAG 1 cut(s) 171
BshNI GGYRCC 1 cut(s) 358
BsiSI CCGG 2 cut(s) 438, 458
BslFI GGGAC 3 cut(s) 540, 566, 853
BslI CCNNNNNNNGG 1 cut(s) 52
BsmAI GTCTC 1 cut(s) 786
BsmFI GGGAC 3 cut(s) 540, 566, 853
Bsp143I GATC 6 cut(s) 112, 292, 442, 597, 856, 862
Bsp19I CCATGG 1 cut(s) 975
BspACI CCGC 4 cut(s) 184, 671, 689, 1071
BspLI GGNNCC 3 cut(s) 105, 256, 360
BspMAI CTGCAG 1 cut(s) 133
BspPI GGATC 1 cut(s) 437
BspT107I GGYRCC 1 cut(s) 358
BsrDI GCAATG 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 931, 975
BssMI GATC 6 cut(s) 112, 292, 442, 597, 856, 862
BssNI GRCGYC 1 cut(s) 14
BssT1I CCWWGG 1 cut(s) 975
Bst2UI CCWGG 3 cut(s) 509, 647, 932
Bst4CI ACNGT 6 cut(s) 269, 464, 613, 731, 873, 1002
BstACI GRCGYC 1 cut(s) 14
BstC8I GCNNGC 3 cut(s) 150, 627, 693
BstDEI CTNAG 1 cut(s) 696
BstDSI CCRYGG 1 cut(s) 975
BstF5I GGATG 1 cut(s) 643
BstKTI GATC 6 cut(s) 115, 295, 445, 600, 859, 865
BstMAI GTCTC 1 cut(s) 786
BstMBI GATC 6 cut(s) 112, 292, 442, 597, 856, 862
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 3 cut(s) 509, 647, 932
BstNSI RCATGY 1 cut(s) 942
BstSCI CCNGG 4 cut(s) 456, 507, 645, 930
BstSFI CTRYAG 3 cut(s) 129, 202, 849
BstV1I GCAGC 2 cut(s) 115, 427
BstV2I GAAGAC 3 cut(s) 53, 507, 1009
BstX2I RGATCY 2 cut(s) 292, 597
BstXI CCANNNNNNTGG 2 cut(s) 269, 1075
BstYI RGATCY 2 cut(s) 292, 597
BtgI CCRYGG 1 cut(s) 975
BtsCI GGATG 1 cut(s) 643
BtsIMutI CAGTG 1 cut(s) 274
Cac8I GCNNGC 3 cut(s) 150, 627, 693
Cfr13I GGNCC 1 cut(s) 750
CsiI ACCWGGT 1 cut(s) 507
Csp6I GTAC 1 cut(s) 998
CviAII CATG 4 cut(s) 706, 939, 976, 1009
CviQI GTAC 1 cut(s) 998
DdeI CTNAG 1 cut(s) 696
DpnI GATC 6 cut(s) 114, 294, 444, 599, 858, 864
DpnII GATC 6 cut(s) 112, 292, 442, 597, 856, 862
DraIII CACNNNGTG 2 cut(s) 511, 775
DrdI GACNNNNNNGTC 1 cut(s) 1013
DseDI GACNNNNNNGTC 1 cut(s) 1013
EciI GGCGGA 2 cut(s) 660, 678
Eco130I CCWWGG 1 cut(s) 975
Eco47I GGWCC 1 cut(s) 750
EcoRII CCWGG 3 cut(s) 507, 645, 930
EcoT14I CCWWGG 1 cut(s) 975
ErhI CCWWGG 1 cut(s) 975
FaeI CATG 4 cut(s) 709, 942, 979, 1012
FaqI GGGAC 3 cut(s) 540, 566, 853
FatI CATG 4 cut(s) 705, 938, 975, 1008
FblI GTMKAC 1 cut(s) 201
Fnu4HI GCNGC 2 cut(s) 129, 416
FokI GGATG 1 cut(s) 650
Fsp4HI GCNGC 2 cut(s) 129, 416
FspBI CTAG 2 cut(s) 240, 296
GluI GCNGC 2 cut(s) 129, 416
GsuI CTGGAG 1 cut(s) 1011
HapII CCGG 2 cut(s) 438, 458
Hin1I GRCGYC 1 cut(s) 14
Hin1II CATG 4 cut(s) 709, 942, 979, 1012
HincII GTYRAC 3 cut(s) 282, 643, 944
HindII GTYRAC 3 cut(s) 282, 643, 944
HinfI GANTC 3 cut(s) 215, 971, 1043
HpaII CCGG 2 cut(s) 438, 458
HphI GGTGA 4 cut(s) 210, 452, 866, 904
Hpy166II GTNNAC 7 cut(s) 202, 282, 643, 727, 831, 869, 944
Hpy188I TCNGA 6 cut(s) 160, 347, 741, 924, 1042, 1052
Hpy188III TCNNGA 6 cut(s) 89, 334, 401, 421, 787, 860
Hpy8I GTNNAC 7 cut(s) 202, 282, 643, 727, 831, 869, 944
HpyAV CCTTC 4 cut(s) 380, 398, 418, 677
HpyCH4III ACNGT 6 cut(s) 269, 464, 613, 731, 873, 1002
HpyCH4IV ACGT 4 cut(s) 14, 409, 567, 827
HpyCH4V TGCA 6 cut(s) 131, 138, 152, 382, 736, 938
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 1 cut(s) 696
HpySE526I ACGT 4 cut(s) 14, 409, 567, 827
Hsp92I GRCGYC 1 cut(s) 14
Hsp92II CATG 4 cut(s) 709, 942, 979, 1012
Kzo9I GATC 6 cut(s) 112, 292, 442, 597, 856, 862
LmnI GCTCC 2 cut(s) 260, 423
Lsp1109I GCAGC 2 cut(s) 115, 427
LweI GCATC 2 cut(s) 565, 745
MabI ACCWGGT 1 cut(s) 507
MaeI CTAG 2 cut(s) 240, 296
MaeII ACGT 4 cut(s) 14, 409, 567, 827
MaeIII GTNAC 4 cut(s) 216, 568, 607, 892
MalI GATC 6 cut(s) 114, 294, 444, 599, 858, 864
MboI GATC 6 cut(s) 112, 292, 442, 597, 856, 862
MboII GAAGA 5 cut(s) 53, 173, 507, 677, 1009
MflI RGATCY 2 cut(s) 292, 597
MluCI AATT 8 cut(s) 235, 383, 491, 681, 743, 884, 949, 981
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 2 cut(s) 282, 615
MnlI CCTC 7 cut(s) 82, 237, 384, 684, 829, 883, 1048
MroXI GAANNNNTTC 1 cut(s) 341
MseI TTAA 6 cut(s) 366, 476, 522, 824, 1064, 1093
MslI CAYNNNNRTG 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 184
MspI CCGG 2 cut(s) 438, 458
MspR9I CCNGG 4 cut(s) 458, 509, 647, 932
MvaI CCWGG 3 cut(s) 509, 647, 932
MwoI GCNNNNNNNGC 1 cut(s) 310
NciI CCSGG 1 cut(s) 458
NcoI CCATGG 1 cut(s) 975
NdeII GATC 6 cut(s) 112, 292, 442, 597, 856, 862
NlaIII CATG 4 cut(s) 709, 942, 979, 1012
NlaIV GGNNCC 3 cut(s) 105, 256, 360
NmuCI GTSAC 2 cut(s) 216, 892
NspI RCATGY 1 cut(s) 942
OliI CACNNNNGTG 1 cut(s) 267
PdmI GAANNNNTTC 1 cut(s) 341
PfeI GAWTC 2 cut(s) 971, 1043
PflFI GACNNNGTC 1 cut(s) 844
PkrI GCNGC 2 cut(s) 130, 417
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PsiI TTATAA 2 cut(s) 168, 564
Psp1406I AACGTT 2 cut(s) 567, 827
Psp6I CCWGG 3 cut(s) 507, 645, 930
PspGI CCWGG 3 cut(s) 507, 645, 930
PspN4I GGNNCC 3 cut(s) 105, 256, 360
PspPI GGNCC 1 cut(s) 750
PstI CTGCAG 1 cut(s) 133
PsuI RGATCY 2 cut(s) 292, 597
PsyI GACNNNGTC 1 cut(s) 844
RsaI GTAC 1 cut(s) 999
RsaNI GTAC 1 cut(s) 998
RseI CAYNNNNRTG 1 cut(s) 267
SaqAI TTAA 6 cut(s) 366, 476, 522, 824, 1064, 1093
SatI GCNGC 2 cut(s) 129, 416
Sau3AI GATC 6 cut(s) 112, 292, 442, 597, 856, 862
Sau96I GGNCC 1 cut(s) 750
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 4 cut(s) 458, 509, 647, 932
SexAI ACCWGGT 1 cut(s) 507
SfaNI GCATC 2 cut(s) 565, 745
SfcI CTRYAG 3 cut(s) 129, 202, 849
SinI GGWCC 1 cut(s) 750
SmiMI CAYNNNNRTG 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 399
SmoI CTYRAG 1 cut(s) 399
Sse9I AATT 8 cut(s) 235, 383, 491, 681, 743, 884, 949, 981
SsiI CCGC 4 cut(s) 184, 671, 689, 1071
SspI AATATT 2 cut(s) 211, 392
SspMI CTAG 2 cut(s) 240, 296
StyD4I CCNGG 4 cut(s) 456, 507, 645, 930
StyI CCWWGG 1 cut(s) 975
TaaI ACNGT 6 cut(s) 269, 464, 613, 731, 873, 1002
TaiI ACGT 4 cut(s) 17, 412, 570, 830
TaqI TCGA 2 cut(s) 859, 879
TasI AATT 8 cut(s) 235, 383, 491, 681, 743, 884, 949, 981
TfiI GAWTC 2 cut(s) 971, 1043
Tru1I TTAA 6 cut(s) 366, 476, 522, 824, 1064, 1093
Tru9I TTAA 6 cut(s) 366, 476, 522, 824, 1064, 1093
TscAI CASTG 1 cut(s) 274
TseFI GTSAC 2 cut(s) 216, 892
TseI GCWGC 2 cut(s) 128, 415
Tsp45I GTSAC 2 cut(s) 216, 892
TspDTI ATGAA 5 cut(s) 110, 186, 536, 694, 772
TspGWI ACGGA 1 cut(s) 822
TspRI CASTG 1 cut(s) 274
Tth111I GACNNNGTC 1 cut(s) 844
VpaK11BI GGWCC 1 cut(s) 750
XapI RAATTY 2 cut(s) 681, 743
XceI RCATGY 1 cut(s) 942
XcmI CCANNNNNNNNNTGG 1 cut(s) 269
XmiI GTMKAC 1 cut(s) 201
XmnI GAANNNNTTC 1 cut(s) 341
XspI CTAG 2 cut(s) 240, 296
ZraI GACGTC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.