FvH4_4g30280

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010559
Physical Location & Seq
Reverse (-)
29996203 .. 29997921
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30280.t1

Sequence Viewer

Length: 1719 bp
ATGGGGAAGGGGAACCATATATTTGCAAGCAGATGGTTTATCTTGCAGAAGATTACTAGCACTTGCAAAAGGAGATTTCATGAAAGTTTGTCCTGTACTACTCTTCCCACATTTGATGCAAAAATCTTAGCAAGAGGTTTCCATGAAGTTTGTGGTGGCGGCACATCATTGACAGAAGAGCTGGAGGACATTCTTGATCCTGAAGATCAAGGGTGGGAGACTAGTACCAACTCTGTGACCTGCATTTACAGGGTTCCTAGCACCATGCGTCAGGTGAACAGGAAGGCGTTTGATCCAAGCATTGTGTCAATTGGTCCGTACCATTATGGCGTTGAAAGCCTGCAAGAAATGGAGAAGCTAAAGCACACATACTTTCGGCGCCTCTTTCAACCAAACTACCACAATCAGTTGGATGATGAAGTAGCAGTCTTGCAGAAGAAAAAGGCGGTAAATGATGCGAAGAAGGCAATGGAGGAATTGGAGGCGGTGGCTAGGAGCTGCTACTCGGAACAATCCAAGCTTAACAGCAAGGAGTTTGTAAAGATGATGTTGATTGATGGTTGCTTCATCATAGGTTTGCTGAGGGACTCATTGCAGTCCGACTTTGAGCACACACCCTCCATCATTCAAAGGTGGATGCTTCCAACTCTGCGCCGAGACTTGATCAAGCTCGAAAATCAGCTTCCCCTCTTTGTATTGCGCAAATTATTTCAGCTAACCCAAGCAGGCACCACTTGTCATGAGAAACCAATTACAGGTTGGGAATTAGAAGCACTAGCCCTTCGCTTCTTTAAGCCGCTGCTTCGAGGACATGATGATGTTCTGACCAGTCCACCGCAGCATTATGCACCAAAACATTGTCAAAGTAGCAGCATCAAGCACTTTCTTGATCTTTTCCACCATAGAATCAGTCCGGACTGCTCCACCCGTTCAGTAGTACAAGTGCCACCGAGAGGGAAGCAAACCAAGCAGATTCTATCAATACAAGAGCTCAAAGAAGCAGGAGTCAAGCTAAAGTTCACAACAGACCCCACTCGCAAGCCACTAGACATCACTTTCGGCCGCGGCTCTTGGTGTTGGGGGAAGGTGCTCACAATCCCTCGTATACATATTGCTGATCACAGAGGCACATTGTTCCGGAACATGGTTGCATTTGAAAAATGTAACCCCAACTGCCACCAAGATGTTACATCCTATTTGTTTTTCCTCGACAACCTCATCAACTCGGCTAAGGATGTAGGGATTCTGCACTACTATGGAGTCCTGCACCACTCTCTAGGCAGCAACAGAGAGGTGGCGAAACTCGTCAACAACCTTTGCAAGGAGGTTAGTCCAGATGTTTCCAAGTCCTACTTGTACAAGGTGGTTAGAGATGTCGATGAGTACTATGGATCCAAATATGGAAAAATAAGAGGCTTCTTGGTACACCACCATTTTAGTAGCTGGTTAGTGGGCATCTCAACTCTTGCAGCATGCTTAGCTCTTTACCTAGCACTCGTTCAGACCGGTTCCTCTGTCGCCAGTGCCAGAGAAAGGTTGCATGAACACACCACATTTAAAGACTTTCTGAAAGAGGCTTTGCTTTATGGTCATATATCTAAGTGGGGATTGGGAAATGCTCGGAACCTCCAACAGAAGGCCGTGGTCAATGGCAGTACCGAGAACATAAATCATCTACCTCGTATTCACATGCAGTACTGTGTATACTACTTGGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

573

Amino Acids

65.45

Weight (kDa)

9.05

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 82 - 497 1.8e-107 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 701
Acc36I ACCTGC 1 cut(s) 248
AccB1I GGYRCC 2 cut(s) 378, 728
AccI GTMKAC 2 cut(s) 1105, 1704
AccII CGCG 1 cut(s) 1065
AccIII TCCGGA 2 cut(s) 913, 1137
AciI CCGC 7 cut(s) 159, 446, 485, 797, 836, 1063, 1065
AclWI GGATC 4 cut(s) 191, 287, 1386, 1399
AcoI YGGCCR 1 cut(s) 1060
AcuI CTGAAG 1 cut(s) 222
AcyI GRCGYC 1 cut(s) 379
AfaI GTAC 9 cut(s) 97, 226, 320, 939, 1358, 1385, 1425, 1657, 1697
AfiI CCNNNNNNNGG 6 cut(s) 755, 953, 1144, 1321, 1533, 1636
AgeI ACCGGT 1 cut(s) 1505
AgsI TTSAA 4 cut(s) 335, 389, 629, 1157
AhlI ACTAGT 1 cut(s) 221
Alw21I GWGCWC 3 cut(s) 612, 993, 1092
Alw26I GTCTC 2 cut(s) 212, 651
AlwI GGATC 4 cut(s) 191, 287, 1386, 1399
Aor13HI TCCGGA 2 cut(s) 913, 1137
AoxI GGCC 2 cut(s) 1060, 1638
ApeKI GCWGC 6 cut(s) 498, 799, 838, 870, 1281, 1469
ArsI GACNNNNNNTTYG 2 cut(s) 1040, 1072
AsiGI ACCGGT 1 cut(s) 1505
AspLEI GCGC 3 cut(s) 381, 654, 702
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 286
AvaII GGWCC 1 cut(s) 314
BamHI GGATCC 1 cut(s) 1391
BanI GGYRCC 2 cut(s) 378, 728
BanII GRGCYC 1 cut(s) 993
Bbv12I GWGCWC 3 cut(s) 612, 993, 1092
BbvCI CCTCAGC 1 cut(s) 581
BbvI GCAGC 6 cut(s) 485, 786, 850, 882, 1293, 1481
BccI CCATC 3 cut(s) 27, 551, 629
BceAI ACGGC 1 cut(s) 1625
BclI TGATCA 2 cut(s) 663, 1117
BcoDI GTCTC 2 cut(s) 212, 651
BcuI ACTAGT 1 cut(s) 221
BfaI CTAG 8 cut(s) 57, 222, 258, 492, 776, 1046, 1277, 1490
BfoI RGCGCY 1 cut(s) 382
BfuAI ACCTGC 1 cut(s) 248
BlpI GCTNAGC 1 cut(s) 1477
BmcAI AGTACT 2 cut(s) 1385, 1697
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 7 cut(s) 14, 255, 380, 730, 1393, 1510, 1625
BmsI GCATC 5 cut(s) 106, 445, 627, 882, 1464
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 2 cut(s) 581, 1230
Bpu1102I GCTNAGC 1 cut(s) 1477
BsaHI GRCGYC 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 1063, 1641
BsaWI WCCGGW 3 cut(s) 913, 1137, 1505
BsaXI ACNNNNNCTCC 2 cut(s) 602, 632
Bsc4I CCNNNNNNNGG 6 cut(s) 755, 953, 1144, 1321, 1533, 1636
Bse118I RCCGGY 1 cut(s) 1505
Bse1I ACTGG 2 cut(s) 828, 1521
Bse3DI GCAATG 2 cut(s) 474, 590
BseAI TCCGGA 2 cut(s) 913, 1137
BseDI CCNNGG 2 cut(s) 1063, 1641
BseGI GGATG 4 cut(s) 418, 642, 1190, 1240
BseLI CCNNNNNNNGG 6 cut(s) 755, 953, 1144, 1321, 1533, 1636
BseMI GCAATG 2 cut(s) 474, 590
BseMII CTCAG 1 cut(s) 572
BseNI ACTGG 2 cut(s) 828, 1521
BseX3I CGGCCG 1 cut(s) 1060
BseXI GCAGC 6 cut(s) 485, 786, 850, 882, 1293, 1481
BsgI GTGCAG 2 cut(s) 1232, 1250
Bsh1236I CGCG 1 cut(s) 1065
Bsh1285I CGRYCG 1 cut(s) 1063
BshFI GGCC 2 cut(s) 1062, 1640
BshNI GGYRCC 2 cut(s) 378, 728
BshTI ACCGGT 1 cut(s) 1505
BsiEI CGRYCG 1 cut(s) 1063
BsiHKAI GWGCWC 3 cut(s) 612, 993, 1092
BsiSI CCGG 3 cut(s) 914, 1138, 1506
BslFI GGGAC 1 cut(s) 599
BslI CCNNNNNNNGG 6 cut(s) 755, 953, 1144, 1321, 1533, 1636
BsmAI GTCTC 2 cut(s) 212, 651
BsmFI GGGAC 1 cut(s) 599
BsnI GGCC 2 cut(s) 1062, 1640
Bsp1286I GDGCHC 3 cut(s) 612, 993, 1092
Bsp13I TCCGGA 2 cut(s) 913, 1137
Bsp1407I TGTACA 1 cut(s) 1356
Bsp143I GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
Bsp1720I GCTNAGC 1 cut(s) 1477
BspACI CCGC 7 cut(s) 159, 446, 485, 797, 836, 1063, 1065
BspANI GGCC 2 cut(s) 1062, 1640
BspCNI CTCAG 1 cut(s) 573
BspEI TCCGGA 2 cut(s) 913, 1137
BspFNI CGCG 1 cut(s) 1065
BspHI TCATGA 2 cut(s) 79, 739
BspLI GGNNCC 7 cut(s) 14, 255, 380, 730, 1393, 1510, 1625
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 4 cut(s) 191, 287, 1386, 1399
BspQI GCTCTTC 1 cut(s) 171
BspT107I GGYRCC 2 cut(s) 378, 728
BsrDI GCAATG 2 cut(s) 474, 590
BsrFI RCCGGY 1 cut(s) 1505
BsrGI TGTACA 1 cut(s) 1356
BsrI ACTGG 2 cut(s) 828, 1521
BssAI RCCGGY 1 cut(s) 1505
BssECI CCNNGG 2 cut(s) 1063, 1641
BssMI GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
BssNAI GTATAC 2 cut(s) 1106, 1705
BssNI GRCGYC 1 cut(s) 379
Bst1107I GTATAC 2 cut(s) 1106, 1705
Bst4CI ACNGT 1 cut(s) 1700
Bst6I CTCTTC 2 cut(s) 108, 171
BstACI GRCGYC 1 cut(s) 379
BstAUI TGTACA 1 cut(s) 1356
BstC8I GCNNGC 5 cut(s) 28, 341, 727, 1040, 1474
BstDEI CTNAG 5 cut(s) 127, 581, 1230, 1477, 1599
BstDSI CCRYGG 2 cut(s) 1063, 1641
BstENI CCTNNNNNAGG 1 cut(s) 1319
BstF5I GGATG 4 cut(s) 418, 642, 1190, 1240
BstFNI CGCG 1 cut(s) 1065
BstH2I RGCGCY 1 cut(s) 382
BstHHI GCGC 3 cut(s) 381, 654, 702
BstKTI GATC 7 cut(s) 199, 208, 295, 666, 892, 1120, 1394
BstMAI GTCTC 2 cut(s) 212, 651
BstMBI GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
BstMCI CGRYCG 1 cut(s) 1063
BstMWI GCNNNNNNNGC 4 cut(s) 336, 464, 967, 1478
BstNSI RCATGY 2 cut(s) 1476, 1693
BstUI CGCG 1 cut(s) 1065
BstV1I GCAGC 6 cut(s) 485, 786, 850, 882, 1293, 1481
BstX2I RGATCY 1 cut(s) 1391
BstYI RGATCY 1 cut(s) 1391
BstZ17I GTATAC 2 cut(s) 1106, 1705
BstZI CGGCCG 1 cut(s) 1060
BsuRI GGCC 2 cut(s) 1062, 1640
BtgI CCRYGG 2 cut(s) 1063, 1641
BtsCI GGATG 4 cut(s) 418, 642, 1190, 1240
BtsIMutI CAGTG 1 cut(s) 1528
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 5 cut(s) 28, 341, 727, 1040, 1474
CciI TCATGA 2 cut(s) 79, 739
CfoI GCGC 3 cut(s) 381, 654, 702
Cfr10I RCCGGY 1 cut(s) 1505
Cfr13I GGNCC 1 cut(s) 314
Cfr42I CCGCGG 1 cut(s) 1066
CseI GACGC 1 cut(s) 257
Csp6I GTAC 9 cut(s) 96, 225, 319, 938, 1357, 1384, 1424, 1656, 1696
CspAI ACCGGT 1 cut(s) 1505
CviAII CATG 9 cut(s) 80, 143, 265, 740, 812, 1144, 1473, 1541, 1690
CviQI GTAC 9 cut(s) 96, 225, 319, 938, 1357, 1384, 1424, 1656, 1696
DdeI CTNAG 5 cut(s) 127, 581, 1230, 1477, 1599
DinI GGCGCC 1 cut(s) 380
DpnI GATC 7 cut(s) 198, 207, 294, 665, 891, 1119, 1393
DpnII GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
DraI TTTAAA 1 cut(s) 1558
EaeI YGGCCR 1 cut(s) 1060
EagI CGGCCG 1 cut(s) 1060
Eam1104I CTCTTC 2 cut(s) 108, 171
EarI CTCTTC 2 cut(s) 108, 171
Ecl136II GAGCTC 1 cut(s) 991
EclXI CGGCCG 1 cut(s) 1060
Eco24I GRGCYC 1 cut(s) 993
Eco47I GGWCC 1 cut(s) 314
Eco52I CGGCCG 1 cut(s) 1060
Eco53kI GAGCTC 1 cut(s) 991
Eco57I CTGAAG 1 cut(s) 222
EcoICRI GAGCTC 1 cut(s) 991
EcoNI CCTNNNNNAGG 1 cut(s) 1319
EcoT38I GRGCYC 1 cut(s) 993
EgeI GGCGCC 1 cut(s) 380
EheI GGCGCC 1 cut(s) 380
FaeI CATG 9 cut(s) 83, 146, 268, 743, 815, 1147, 1476, 1544, 1693
FalI AAGNNNNNCTT 2 cut(s) 1337, 1369
FaqI GGGAC 1 cut(s) 599
FatI CATG 9 cut(s) 79, 142, 264, 739, 811, 1143, 1472, 1540, 1689
FbaI TGATCA 2 cut(s) 663, 1117
FblI GTMKAC 2 cut(s) 1105, 1704
FokI GGATG 4 cut(s) 425, 649, 1177, 1247
FriOI GRGCYC 1 cut(s) 993
FspBI CTAG 8 cut(s) 57, 222, 258, 492, 776, 1046, 1277, 1490
FspI TGCGCA 1 cut(s) 701
GlaI GCGC 3 cut(s) 380, 653, 701
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 382
HaeIII GGCC 2 cut(s) 1062, 1640
HapII CCGG 3 cut(s) 914, 1138, 1506
HgaI GACGC 1 cut(s) 257
HhaI GCGC 3 cut(s) 381, 654, 702
Hin1I GRCGYC 1 cut(s) 379
Hin1II CATG 9 cut(s) 83, 146, 268, 743, 815, 1147, 1476, 1544, 1693
Hin6I GCGC 3 cut(s) 379, 652, 700
HinP1I GCGC 3 cut(s) 379, 652, 700
HincII GTYRAC 1 cut(s) 1309
HindII GTYRAC 1 cut(s) 1309
HindIII AAGCTT 1 cut(s) 518
HinfI GANTC 6 cut(s) 587, 906, 973, 1005, 1243, 1260
HpaII CCGG 3 cut(s) 914, 1138, 1506
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 7 cut(s) 277, 833, 1020, 1106, 1309, 1426, 1705
Hpy188I TCNGA 6 cut(s) 508, 601, 825, 1503, 1569, 1623
Hpy188III TCNNGA 8 cut(s) 80, 194, 200, 740, 887, 914, 1138, 1334
Hpy8I GTNNAC 7 cut(s) 277, 833, 1020, 1106, 1309, 1426, 1705
HpyAV CCTTC 5 cut(s) 277, 457, 791, 1078, 1630
HpyCH4III ACNGT 1 cut(s) 1700
HpyF10VI GCNNNNNNNGC 4 cut(s) 336, 464, 967, 1478
HpyF3I CTNAG 5 cut(s) 127, 581, 1230, 1477, 1599
Hsp92I GRCGYC 1 cut(s) 379
Hsp92II CATG 9 cut(s) 83, 146, 268, 743, 815, 1147, 1476, 1544, 1693
HspAI GCGC 3 cut(s) 379, 652, 700
KasI GGCGCC 1 cut(s) 378
Kpn2I TCCGGA 2 cut(s) 913, 1137
Ksp22I TGATCA 2 cut(s) 663, 1117
KspI CCGCGG 1 cut(s) 1066
Kzo9I GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 2 cut(s) 495, 926
Lsp1109I GCAGC 6 cut(s) 485, 786, 850, 882, 1293, 1481
LweI GCATC 5 cut(s) 106, 445, 627, 882, 1464
MaeI CTAG 8 cut(s) 57, 222, 258, 492, 776, 1046, 1277, 1490
MaeIII GTNAC 3 cut(s) 235, 1163, 1186
MalI GATC 7 cut(s) 198, 207, 294, 665, 891, 1119, 1393
MboI GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
MboII GAAGA 6 cut(s) 61, 95, 188, 215, 448, 472
MfeI CAATTG 1 cut(s) 309
MflI RGATCY 1 cut(s) 1391
MhlI GDGCHC 3 cut(s) 612, 993, 1092
MluCI AATT 5 cut(s) 309, 476, 704, 750, 764
Mly113I GGCGCC 1 cut(s) 379
MlyI GAGTC 3 cut(s) 581, 1014, 1269
MmeI TCCRAC 4 cut(s) 390, 624, 668, 1654
MroI TCCGGA 2 cut(s) 913, 1137
MseI TTAA 3 cut(s) 522, 792, 1557
MslI CAYNNNNRTG 3 cut(s) 816, 1182, 1254
MspA1I CMGCKG 2 cut(s) 799, 1065
MspI CCGG 3 cut(s) 914, 1138, 1506
MunI CAATTG 1 cut(s) 309
MvnI CGCG 1 cut(s) 1065
MwoI GCNNNNNNNGC 4 cut(s) 336, 464, 967, 1478
NarI GGCGCC 1 cut(s) 379
NdeII GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
NlaIII CATG 9 cut(s) 83, 146, 268, 743, 815, 1147, 1476, 1544, 1693
NlaIV GGNNCC 7 cut(s) 14, 255, 380, 730, 1393, 1510, 1625
NmeAIII GCCGAG 2 cut(s) 680, 1205
NmuCI GTSAC 1 cut(s) 235
NsbI TGCGCA 1 cut(s) 701
NspI RCATGY 2 cut(s) 1476, 1693
PaeI GCATGC 1 cut(s) 1476
PagI TCATGA 2 cut(s) 79, 739
PciSI GCTCTTC 1 cut(s) 171
PfeI GAWTC 3 cut(s) 906, 973, 1243
PinAI ACCGGT 1 cut(s) 1505
PleI GAGTC 3 cut(s) 581, 1013, 1268
PluTI GGCGCC 1 cut(s) 382
PpsI GAGTC 3 cut(s) 581, 1013, 1268
Psp124BI GAGCTC 1 cut(s) 993
PspN4I GGNNCC 7 cut(s) 14, 255, 380, 730, 1393, 1510, 1625
PspPI GGNCC 1 cut(s) 314
PsuI RGATCY 1 cut(s) 1391
RsaI GTAC 9 cut(s) 97, 226, 320, 939, 1358, 1385, 1425, 1657, 1697
RsaNI GTAC 9 cut(s) 96, 225, 319, 938, 1357, 1384, 1424, 1656, 1696
RseI CAYNNNNRTG 3 cut(s) 816, 1182, 1254
SacI GAGCTC 1 cut(s) 993
SacII CCGCGG 1 cut(s) 1066
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 3 cut(s) 522, 792, 1557
Sau3AI GATC 7 cut(s) 196, 205, 292, 663, 889, 1117, 1391
Sau96I GGNCC 1 cut(s) 314
ScaI AGTACT 2 cut(s) 1385, 1697
SchI GAGTC 3 cut(s) 581, 1014, 1269
SduI GDGCHC 3 cut(s) 612, 993, 1092
SfaNI GCATC 5 cut(s) 106, 445, 627, 882, 1464
SfoI GGCGCC 1 cut(s) 380
Sfr303I CCGCGG 1 cut(s) 1066
SgrBI CCGCGG 1 cut(s) 1066
SinI GGWCC 1 cut(s) 314
SmiMI CAYNNNNRTG 3 cut(s) 816, 1182, 1254
SpeI ACTAGT 1 cut(s) 221
SphI GCATGC 1 cut(s) 1476
Sse9I AATT 5 cut(s) 309, 476, 704, 750, 764
SsiI CCGC 7 cut(s) 159, 446, 485, 797, 836, 1063, 1065
SspDI GGCGCC 1 cut(s) 378
SspMI CTAG 8 cut(s) 57, 222, 258, 492, 776, 1046, 1277, 1490
SstI GAGCTC 1 cut(s) 993
TaaI ACNGT 1 cut(s) 1700
TaqI TCGA 4 cut(s) 672, 805, 1209, 1377
TasI AATT 5 cut(s) 309, 476, 704, 750, 764
TatI WGTACW 5 cut(s) 95, 937, 1356, 1383, 1695
TauI GCSGC 4 cut(s) 162, 799, 1065, 1068
TfiI GAWTC 3 cut(s) 906, 973, 1243
Tru1I TTAA 3 cut(s) 522, 792, 1557
Tru9I TTAA 3 cut(s) 522, 792, 1557
TscAI CASTG 1 cut(s) 1528
TseFI GTSAC 1 cut(s) 235
TseI GCWGC 6 cut(s) 498, 799, 838, 870, 1281, 1469
Tsp45I GTSAC 1 cut(s) 235
TspDTI ATGAA 6 cut(s) 68, 96, 159, 432, 556, 1557
TspGWI ACGGA 1 cut(s) 306
TspRI CASTG 1 cut(s) 1528
VpaK11BI GGWCC 1 cut(s) 314
XagI CCTNNNNNAGG 1 cut(s) 1319
XceI RCATGY 2 cut(s) 1476, 1693
XcmI CCANNNNNNNNNTGG 2 cut(s) 149, 756
XmiI GTMKAC 2 cut(s) 1105, 1704
XspI CTAG 8 cut(s) 57, 222, 258, 492, 776, 1046, 1277, 1490
ZrmI AGTACT 2 cut(s) 1385, 1697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.