MD04G1048500.v1.1

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010559
Physical Location & Seq
Forward (+)
5704441 .. 5705211
771 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1048500.v1.1.491

Sequence Viewer

Length: 771 bp
ATGACGAAATTTCATGGTTTGGCGGCCGGAAGGGGAACAAGAGCTGCTGCGCTTCACGTAATCAAAGCGAGAAGTTTCAGCGGCTCGTCAAACACGAAAGCTTTGCTAAAACAAGAATTGCAGAGTGAGATTCCTGGAGAGAGTAGCTCTGTCTGCATTTACAAAATTCCTAATACATTTCGCCAAGTAAAAAGCAAGGCTTACCAACCAAGTATTGTCTCCATTGGCCCTTACCATCATGGACTGCCGAGCTTACAGGAAATGGAAAAACTGAAGCGAGTATACTTTCACCGCGTCTTTAAACCAAAGCCAGAAGAACTGGATCAGGCGACCGAGGCAATGCAGAAACTAGAGGAGGCGGCTCGGAGCTGCTACTCCGAAGAATCCAAGCTATGCAGCGAGGAATTTGTCAAGATGATGCTCATTGATGGTTGCTTCATCGTAGAGTTGCTCAGAGACTCGATGCAACAAGATTTCGTACATACACCCTCCACCATTAAAAGGTGGATGCTGCCAACTCTCCGTCAGGATTTAATCAAGCTTGAAAATCAGCTCCCTCTGTTTGTTTTACGTGAATTGTTTCAGCTGACAGCGAGAAGCTCTTGTTTTTCTTCTGAGCACGAGCAACCAGCATCTTTGGAACTGGAAGCCCTTGCCCTTCGCTTCATTAACCCGCTACTGCAAGGACATCTAGATCCGAACAAACCCTTGGAACATTTTACGCCAAAACAGTTACAAGCACAAGCACTTTCTTCATCTTTTCCACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

257

Amino Acids

29.06

Weight (kDa)

8.54

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 53 - 234 7.5e-51 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 282
AccII CGCG 1 cut(s) 294
AciI CCGC 5 cut(s) 23, 81, 292, 359, 674
AclWI GGATC 2 cut(s) 330, 689
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 3 cut(s) 8, 165, 404
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 1 cut(s) 480
AfiI CCNNNNNNNGG 1 cut(s) 501
AgsI TTSAA 1 cut(s) 545
AjnI CCWGG 1 cut(s) 133
AjuI GAANNNNNNNTTGG 2 cut(s) 692, 724
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 2 cut(s) 223, 450
AlwI GGATC 2 cut(s) 330, 689
AoxI GGCC 2 cut(s) 24, 226
ApeKI GCWGC 5 cut(s) 44, 47, 369, 396, 511
ApoI RAATTY 3 cut(s) 8, 165, 404
Asp700I GAANNNNTTC 1 cut(s) 579
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 1 cut(s) 227
AsuHPI GGTGA 1 cut(s) 281
BarI GAAGNNNNNNTAC 2 cut(s) 266, 298
BauI CACGAG 1 cut(s) 620
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 5 cut(s) 31, 34, 356, 408, 498
BccI CCATC 2 cut(s) 243, 422
BcgI CGANNNNNNTGC 2 cut(s) 85, 119
BciT130I CCWGG 1 cut(s) 135
BcoDI GTCTC 2 cut(s) 223, 450
BfaI CTAG 2 cut(s) 350, 692
BisI GCNGC 8 cut(s) 24, 45, 48, 82, 360, 370, 397, 512
BlsI GCNGC 8 cut(s) 25, 46, 49, 83, 361, 371, 398, 513
Bme1390I CCNGG 1 cut(s) 135
BmgT120I GGNCC 1 cut(s) 227
BmrFI CCNGG 1 cut(s) 135
BmsI GCATC 4 cut(s) 408, 453, 498, 641
BplI GAGNNNNNCTC 2 cut(s) 131, 163
BpmI CTGGAG 1 cut(s) 156
BsaAI YACGTR 2 cut(s) 58, 572
BsaJI CCNNGG 2 cut(s) 333, 708
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse1I ACTGG 2 cut(s) 324, 648
Bse3DI GCAATG 1 cut(s) 345
BseBI CCWGG 1 cut(s) 135
BseDI CCNNGG 2 cut(s) 333, 708
BseGI GGATG 1 cut(s) 513
BseLI CCNNNNNNNGG 1 cut(s) 501
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 2 cut(s) 466, 606
BseNI ACTGG 2 cut(s) 324, 648
BseRI GAGGAG 1 cut(s) 368
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 5 cut(s) 31, 34, 356, 408, 498
Bsh1236I CGCG 1 cut(s) 294
Bsh1285I CGRYCG 2 cut(s) 27, 333
BshFI GGCC 2 cut(s) 26, 228
BsiEI CGRYCG 2 cut(s) 27, 333
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 1 cut(s) 27
BslI CCNNNNNNNGG 1 cut(s) 501
BsmAI GTCTC 2 cut(s) 223, 450
BsnI GGCC 2 cut(s) 26, 228
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 2 cut(s) 322, 694
BspACI CCGC 5 cut(s) 23, 81, 292, 359, 674
BspANI GGCC 2 cut(s) 26, 228
BspCNI CTCAG 2 cut(s) 465, 607
BspFNI CGCG 1 cut(s) 294
BspPI GGATC 2 cut(s) 330, 689
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 2 cut(s) 324, 648
BssECI CCNNGG 2 cut(s) 333, 708
BssMI GATC 2 cut(s) 322, 694
BssNAI GTATAC 1 cut(s) 283
BssSI CACGAG 1 cut(s) 620
BssT1I CCWWGG 1 cut(s) 708
Bst1107I GTATAC 1 cut(s) 283
Bst2BI CACGAG 1 cut(s) 620
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 1 cut(s) 732
BstBAI YACGTR 2 cut(s) 58, 572
BstDEI CTNAG 2 cut(s) 452, 615
BstF5I GGATG 1 cut(s) 513
BstFNI CGCG 1 cut(s) 294
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 2 cut(s) 325, 697
BstMAI GTCTC 2 cut(s) 223, 450
BstMBI GATC 2 cut(s) 322, 694
BstMCI CGRYCG 2 cut(s) 27, 333
BstMWI GCNNNNNNNGC 2 cut(s) 153, 335
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 1 cut(s) 133
BstUI CGCG 1 cut(s) 294
BstV1I GCAGC 5 cut(s) 31, 34, 356, 408, 498
BstX2I RGATCY 1 cut(s) 694
BstYI RGATCY 1 cut(s) 694
BstZ17I GTATAC 1 cut(s) 283
BstZI CGGCCG 1 cut(s) 24
BsuRI GGCC 2 cut(s) 26, 228
BtsCI GGATG 1 cut(s) 513
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 1 cut(s) 227
CseI GACGC 1 cut(s) 283
Csp6I GTAC 1 cut(s) 479
CviAII CATG 2 cut(s) 14, 239
CviQI GTAC 1 cut(s) 479
DdeI CTNAG 2 cut(s) 452, 615
DpnI GATC 2 cut(s) 324, 696
DpnII GATC 2 cut(s) 322, 694
DraI TTTAAA 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 24
EagI CGGCCG 1 cut(s) 24
EclXI CGGCCG 1 cut(s) 24
Eco130I CCWWGG 1 cut(s) 708
Eco52I CGGCCG 1 cut(s) 24
Eco57I CTGAAG 1 cut(s) 293
EcoRII CCWGG 1 cut(s) 133
EcoT14I CCWWGG 1 cut(s) 708
ErhI CCWWGG 1 cut(s) 708
FaeI CATG 2 cut(s) 17, 242
FaiI YATR 6 cut(s) 15, 240, 283, 394, 483, 769
FalI AAGNNNNNCTT 2 cut(s) 184, 216
FatI CATG 2 cut(s) 13, 238
FauI CCCGC 1 cut(s) 681
FblI GTMKAC 1 cut(s) 282
Fnu4HI GCNGC 8 cut(s) 24, 45, 48, 82, 360, 370, 397, 512
FokI GGATG 1 cut(s) 520
Fsp4HI GCNGC 8 cut(s) 24, 45, 48, 82, 360, 370, 397, 512
FspBI CTAG 2 cut(s) 350, 692
GlaI GCGC 1 cut(s) 51
GluI GCNGC 8 cut(s) 24, 45, 48, 82, 360, 370, 397, 512
GsuI CTGGAG 1 cut(s) 156
HaeIII GGCC 2 cut(s) 26, 228
HapII CCGG 1 cut(s) 27
HgaI GACGC 1 cut(s) 283
HhaI GCGC 1 cut(s) 52
Hin1II CATG 2 cut(s) 17, 242
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HindIII AAGCTT 2 cut(s) 99, 539
HinfI GANTC 3 cut(s) 130, 383, 458
HpaII CCGG 1 cut(s) 27
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 1 cut(s) 283
Hpy188I TCNGA 5 cut(s) 366, 379, 455, 616, 699
Hpy188III TCNNGA 3 cut(s) 412, 527, 692
Hpy8I GTNNAC 1 cut(s) 283
HpyAV CCTTC 2 cut(s) 24, 668
HpyCH4III ACNGT 1 cut(s) 732
HpyCH4IV ACGT 2 cut(s) 57, 571
HpyCH4V TGCA 6 cut(s) 121, 156, 343, 396, 466, 682
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 335
HpyF3I CTNAG 2 cut(s) 452, 615
HpySE526I ACGT 2 cut(s) 57, 571
Hsp92II CATG 2 cut(s) 17, 242
HspAI GCGC 1 cut(s) 50
Kzo9I GATC 2 cut(s) 322, 694
LmnI GCTCC 2 cut(s) 366, 558
Lsp1109I GCAGC 5 cut(s) 31, 34, 356, 408, 498
LweI GCATC 4 cut(s) 408, 453, 498, 641
MaeI CTAG 2 cut(s) 350, 692
MaeII ACGT 2 cut(s) 57, 571
MaeIII GTNAC 1 cut(s) 732
MalI GATC 2 cut(s) 324, 696
MboI GATC 2 cut(s) 322, 694
MboII GAAGA 4 cut(s) 326, 392, 603, 744
MflI RGATCY 1 cut(s) 694
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 5 cut(s) 8, 116, 165, 404, 575
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 6 cut(s) 328, 346, 349, 394, 499, 567
MroXI GAANNNNTTC 1 cut(s) 579
MseI TTAA 4 cut(s) 300, 498, 533, 669
MspA1I CMGCKG 2 cut(s) 81, 586
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 1 cut(s) 135
MvaI CCWGG 1 cut(s) 135
MvnI CGCG 1 cut(s) 294
MwoI GCNNNNNNNGC 2 cut(s) 153, 335
NdeII GATC 2 cut(s) 322, 694
NlaIII CATG 2 cut(s) 17, 242
NmeAIII GCCGAG 1 cut(s) 273
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 2 cut(s) 130, 383
PfoI TCCNGGA 1 cut(s) 133
PkrI GCNGC 8 cut(s) 25, 46, 49, 83, 361, 371, 398, 513
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
Ppu21I YACGTR 2 cut(s) 58, 572
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspPI GGNCC 1 cut(s) 227
PsuI RGATCY 1 cut(s) 694
PvuII CAGCTG 1 cut(s) 586
RsaI GTAC 1 cut(s) 480
RsaNI GTAC 1 cut(s) 479
SaqAI TTAA 4 cut(s) 300, 498, 533, 669
SatI GCNGC 8 cut(s) 24, 45, 48, 82, 360, 370, 397, 512
Sau3AI GATC 2 cut(s) 322, 694
Sau96I GGNCC 1 cut(s) 227
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 1 cut(s) 135
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 4 cut(s) 408, 453, 498, 641
Sse9I AATT 5 cut(s) 8, 116, 165, 404, 575
SsiI CCGC 5 cut(s) 23, 81, 292, 359, 674
SspMI CTAG 2 cut(s) 350, 692
StyD4I CCNGG 1 cut(s) 133
StyI CCWWGG 1 cut(s) 708
TaaI ACNGT 1 cut(s) 732
TaiI ACGT 2 cut(s) 60, 574
TaqI TCGA 1 cut(s) 461
TaqII GACCGA 1 cut(s) 347
TasI AATT 5 cut(s) 8, 116, 165, 404, 575
TauI GCSGC 3 cut(s) 26, 84, 362
TfiI GAWTC 2 cut(s) 130, 383
Tru1I TTAA 4 cut(s) 300, 498, 533, 669
Tru9I TTAA 4 cut(s) 300, 498, 533, 669
TseI GCWGC 5 cut(s) 44, 47, 369, 396, 511
TspDTI ATGAA 3 cut(s) 427, 655, 744
TspGWI ACGGA 1 cut(s) 512
XapI RAATTY 3 cut(s) 8, 165, 404
XbaI TCTAGA 1 cut(s) 691
XmiI GTMKAC 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 2 cut(s) 350, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.