Rroxscaffold_152G00434540

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0010559
Physical Location & Seq
Forward (+)
69912 .. 70768
857 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434540.1

Sequence Viewer

Length: 729 bp
ATGCACCACAACATTGTCAAAGCAGCAGCAGCAAGCACTTTCTTGATCTTTTCCACAGGACAATCAGTCCGGACTGCTCTACCCGTTCGACTGCAAGCGCCGCGGCGAGGGAAGCAAACCAAGCAGATTCTATCGGTACAAGAGCTCAAGGAGGCCGGAGTCAAGTTCAGAACAGACCCCACTCGAGGGCCACTAGACATCACTTTCGGCCGCGTCGCTTGGAGTTGGGGGAAGCCTCATCAACTCGGCCAAGGATGTAGGGCTCTGCACTACCACGGAGTCCTGCAACACTCTCTAGGAAGCAACAGGAGGGTGGCGAAACTCGTCAACAACCTTTGCAAGGAGGTTGGTCCGGATGTTTCCAAGTCCTACTTGTACAAGGTGGTTAGAGATGTCGATGAGTACTATAGTTCCAAGTATGGAAAAATAAGAGGCTTCTTGGTACACCATCATTTTAGTAGCTGGTTGGTGGGCATCTCAACTCTTGCAGCATGCTTAGCTCTTTACCTAGCATTCATTCAGACCGGCTGCACAGTCGCTACTGCCAAAGACGAATTGAGTAGACATACCACATTTGGAGACTTTCTGAAAGAGTCTTTGCTTTTGCATCATATGAAACAGTGGTGGGGATCATTTGGAAATACCGGCAGTACTGAGAATAACGCCCTTGATCGCAATGACATGGAGTACTGTGTATACTACTTGACATGGGAATGGACTTTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

242

Amino Acids

27.42

Weight (kDa)

9.51

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 88 - 170 7.9e-16 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 562, 696
AccII CGCG 2 cut(s) 103, 213
AccIII TCCGGA 2 cut(s) 69, 352
AciI CCGC 3 cut(s) 101, 103, 211
AclWI GGATC 1 cut(s) 637
AcoI YGGCCR 2 cut(s) 208, 247
AfaI GTAC 6 cut(s) 138, 377, 404, 444, 652, 689
AfiI CCNNNNNNNGG 4 cut(s) 107, 185, 186, 340
AhdI GACNNNNNGTC 1 cut(s) 65
AluBI AGCT 3 cut(s) 145, 462, 500
AluI AGCT 3 cut(s) 145, 462, 500
Alw21I GWGCWC 1 cut(s) 147
Alw26I GTCTC 1 cut(s) 573
AlwI GGATC 1 cut(s) 637
Ama87I CYCGRG 1 cut(s) 183
Aor13HI TCCGGA 2 cut(s) 69, 352
AoxI GGCC 4 cut(s) 153, 188, 208, 247
ApeKI GCWGC 5 cut(s) 23, 26, 29, 488, 528
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 2 cut(s) 188, 350
AvaI CYCGRG 1 cut(s) 183
AvaII GGWCC 1 cut(s) 350
BanII GRGCYC 2 cut(s) 147, 265
Bbv12I GWGCWC 1 cut(s) 147
BbvI GCAGC 5 cut(s) 35, 38, 41, 500, 515
BccI CCATC 1 cut(s) 456
BcoDI GTCTC 1 cut(s) 573
BfaI CTAG 3 cut(s) 194, 296, 509
BfmI CTRYAG 1 cut(s) 406
BfoI RGCGCY 1 cut(s) 101
BisI GCNGC 8 cut(s) 24, 27, 30, 101, 104, 211, 489, 529
BlpI GCTNAGC 1 cut(s) 496
BlsI GCNGC 8 cut(s) 25, 28, 31, 102, 105, 212, 490, 530
BmcAI AGTACT 3 cut(s) 404, 652, 689
Bme18I GGWCC 1 cut(s) 350
BmeRI GACNNNNNGTC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 183
BmgT120I GGNCC 2 cut(s) 188, 350
BmsI GCATC 2 cut(s) 483, 616
Bpu1102I GCTNAGC 1 cut(s) 496
BpuEI CTTGAG 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 101, 250, 274
BsaWI WCCGGW 2 cut(s) 69, 352
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 185, 186, 340
Bse118I RCCGGY 2 cut(s) 524, 644
Bse3DI GCAATG 1 cut(s) 682
BseAI TCCGGA 2 cut(s) 69, 352
BseDI CCNNGG 3 cut(s) 101, 250, 274
BseGI GGATG 2 cut(s) 260, 361
BseLI CCNNNNNNNGG 4 cut(s) 107, 185, 186, 340
BseMI GCAATG 1 cut(s) 682
BseMII CTCAG 1 cut(s) 645
BseX3I CGGCCG 1 cut(s) 208
BseXI GCAGC 5 cut(s) 35, 38, 41, 500, 515
BsgI GTGCAG 2 cut(s) 251, 514
Bsh1236I CGCG 2 cut(s) 103, 213
Bsh1285I CGRYCG 1 cut(s) 211
BshFI GGCC 4 cut(s) 155, 190, 210, 249
BsiEI CGRYCG 1 cut(s) 211
BsiHKAI GWGCWC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 183
BsiSI CCGG 5 cut(s) 70, 156, 353, 525, 645
BslI CCNNNNNNNGG 4 cut(s) 107, 185, 186, 340
BsmAI GTCTC 1 cut(s) 573
BsmI GAATGC 1 cut(s) 512
BsnI GGCC 4 cut(s) 155, 190, 210, 249
BsoBI CYCGRG 1 cut(s) 183
Bsp1286I GDGCHC 2 cut(s) 147, 265
Bsp13I TCCGGA 2 cut(s) 69, 352
Bsp1407I TGTACA 1 cut(s) 375
Bsp143I GATC 3 cut(s) 45, 629, 670
Bsp1720I GCTNAGC 1 cut(s) 496
BspACI CCGC 3 cut(s) 101, 103, 211
BspANI GGCC 4 cut(s) 155, 190, 210, 249
BspCNI CTCAG 1 cut(s) 646
BspEI TCCGGA 2 cut(s) 69, 352
BspFNI CGCG 2 cut(s) 103, 213
BspPI GGATC 1 cut(s) 637
BsrDI GCAATG 1 cut(s) 682
BsrFI RCCGGY 2 cut(s) 524, 644
BsrGI TGTACA 1 cut(s) 375
BssAI RCCGGY 2 cut(s) 524, 644
BssECI CCNNGG 3 cut(s) 101, 250, 274
BssMI GATC 3 cut(s) 45, 629, 670
BssNAI GTATAC 1 cut(s) 697
BssT1I CCWWGG 1 cut(s) 250
Bst1107I GTATAC 1 cut(s) 697
Bst4CI ACNGT 3 cut(s) 535, 621, 692
BstAUI TGTACA 1 cut(s) 375
BstC8I GCNNGC 3 cut(s) 34, 96, 493
BstDEI CTNAG 2 cut(s) 496, 654
BstDSI CCRYGG 2 cut(s) 101, 274
BstENI CCTNNNNNAGG 1 cut(s) 338
BstF5I GGATG 2 cut(s) 260, 361
BstFNI CGCG 2 cut(s) 103, 213
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 3 cut(s) 48, 632, 673
BstMAI GTCTC 1 cut(s) 573
BstMBI GATC 3 cut(s) 45, 629, 670
BstMCI CGRYCG 1 cut(s) 211
BstMWI GCNNNNNNNGC 5 cut(s) 29, 100, 112, 121, 497
BstNSI RCATGY 1 cut(s) 495
BstSFI CTRYAG 1 cut(s) 406
BstUI CGCG 2 cut(s) 103, 213
BstV1I GCAGC 5 cut(s) 35, 38, 41, 500, 515
BstZ17I GTATAC 1 cut(s) 697
BstZI CGGCCG 1 cut(s) 208
BsuRI GGCC 4 cut(s) 155, 190, 210, 249
BtgI CCRYGG 2 cut(s) 101, 274
BtsCI GGATG 2 cut(s) 260, 361
BtsIMutI CAGTG 1 cut(s) 626
Cac8I GCNNGC 3 cut(s) 34, 96, 493
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 2 cut(s) 524, 644
Cfr13I GGNCC 2 cut(s) 188, 350
Cfr42I CCGCGG 1 cut(s) 104
CseI GACGC 1 cut(s) 202
Csp6I GTAC 6 cut(s) 137, 376, 403, 443, 651, 688
CviAII CATG 3 cut(s) 492, 682, 708
CviQI GTAC 6 cut(s) 137, 376, 403, 443, 651, 688
DdeI CTNAG 2 cut(s) 496, 654
DpnI GATC 3 cut(s) 47, 631, 672
DpnII GATC 3 cut(s) 45, 629, 670
DriI GACNNNNNGTC 1 cut(s) 65
EaeI YGGCCR 2 cut(s) 208, 247
EagI CGGCCG 1 cut(s) 208
Eam1105I GACNNNNNGTC 1 cut(s) 65
Ecl136II GAGCTC 1 cut(s) 145
EclXI CGGCCG 1 cut(s) 208
Eco130I CCWWGG 1 cut(s) 250
Eco24I GRGCYC 2 cut(s) 147, 265
Eco47I GGWCC 1 cut(s) 350
Eco52I CGGCCG 1 cut(s) 208
Eco53kI GAGCTC 1 cut(s) 145
Eco88I CYCGRG 1 cut(s) 183
EcoICRI GAGCTC 1 cut(s) 145
EcoNI CCTNNNNNAGG 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 250
EcoT38I GRGCYC 2 cut(s) 147, 265
ErhI CCWWGG 1 cut(s) 250
FaeI CATG 3 cut(s) 495, 685, 711
FaiI YATR 9 cut(s) 408, 420, 493, 567, 612, 614, 683, 697, 709
FalI AAGNNNNNCTT 2 cut(s) 356, 388
FatI CATG 3 cut(s) 491, 681, 707
FauNDI CATATG 1 cut(s) 612
FblI GTMKAC 2 cut(s) 562, 696
Fnu4HI GCNGC 8 cut(s) 24, 27, 30, 101, 104, 211, 489, 529
FokI GGATG 2 cut(s) 267, 368
FriOI GRGCYC 2 cut(s) 147, 265
Fsp4HI GCNGC 8 cut(s) 24, 27, 30, 101, 104, 211, 489, 529
FspBI CTAG 3 cut(s) 194, 296, 509
GlaI GCGC 1 cut(s) 99
GluI GCNGC 8 cut(s) 24, 27, 30, 101, 104, 211, 489, 529
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 4 cut(s) 155, 190, 210, 249
HapII CCGG 5 cut(s) 70, 156, 353, 525, 645
HgaI GACGC 1 cut(s) 202
HhaI GCGC 1 cut(s) 100
Hin1II CATG 3 cut(s) 495, 685, 711
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HincII GTYRAC 1 cut(s) 328
HindII GTYRAC 1 cut(s) 328
HinfI GANTC 4 cut(s) 127, 159, 279, 593
HpaII CCGG 5 cut(s) 70, 156, 353, 525, 645
Hpy166II GTNNAC 4 cut(s) 328, 445, 563, 697
Hpy188I TCNGA 3 cut(s) 170, 522, 588
Hpy188III TCNNGA 3 cut(s) 43, 70, 353
Hpy8I GTNNAC 4 cut(s) 328, 445, 563, 697
Hpy99I CGWCG 1 cut(s) 218
HpyCH4III ACNGT 3 cut(s) 535, 621, 692
HpyCH4V TGCA 8 cut(s) 4, 94, 268, 286, 339, 488, 531, 607
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 100, 112, 121, 497
HpyF3I CTNAG 2 cut(s) 496, 654
Hsp92II CATG 3 cut(s) 495, 685, 711
HspAI GCGC 1 cut(s) 98
Kpn2I TCCGGA 2 cut(s) 69, 352
KspI CCGCGG 1 cut(s) 104
Kzo9I GATC 3 cut(s) 45, 629, 670
LpnPI CCDG 9 cut(s) 42, 83, 169, 292, 296, 366, 448, 538, 658
Lsp1109I GCAGC 5 cut(s) 35, 38, 41, 500, 515
LweI GCATC 2 cut(s) 483, 616
MaeI CTAG 3 cut(s) 194, 296, 509
MalI GATC 3 cut(s) 47, 631, 672
MboI GATC 3 cut(s) 45, 629, 670
MhlI GDGCHC 2 cut(s) 147, 265
MluCI AATT 2 cut(s) 554, 724
MlyI GAGTC 3 cut(s) 168, 288, 602
MnlI CCTC 7 cut(s) 101, 145, 179, 246, 303, 337, 425
MroI TCCGGA 2 cut(s) 69, 352
MseI TTAA 1 cut(s) 723
MslI CAYNNNNRTG 1 cut(s) 712
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 5 cut(s) 70, 156, 353, 525, 645
Mva1269I GAATGC 1 cut(s) 512
MvnI CGCG 2 cut(s) 103, 213
MwoI GCNNNNNNNGC 5 cut(s) 29, 100, 112, 121, 497
NdeI CATATG 1 cut(s) 612
NdeII GATC 3 cut(s) 45, 629, 670
NlaIII CATG 3 cut(s) 495, 685, 711
NmeAIII GCCGAG 1 cut(s) 225
NspI RCATGY 1 cut(s) 495
PaeI GCATGC 1 cut(s) 495
PaeR7I CTCGAG 1 cut(s) 183
PctI GAATGC 1 cut(s) 512
PfeI GAWTC 1 cut(s) 127
PkrI GCNGC 8 cut(s) 25, 28, 31, 102, 105, 212, 490, 530
PleI GAGTC 3 cut(s) 167, 287, 601
PpsI GAGTC 3 cut(s) 167, 287, 601
Psp124BI GAGCTC 1 cut(s) 147
PspPI GGNCC 2 cut(s) 188, 350
PspXI VCTCGAGB 1 cut(s) 183
RsaI GTAC 6 cut(s) 138, 377, 404, 444, 652, 689
RsaNI GTAC 6 cut(s) 137, 376, 403, 443, 651, 688
RseI CAYNNNNRTG 1 cut(s) 712
SacI GAGCTC 1 cut(s) 147
SacII CCGCGG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 723
SatI GCNGC 8 cut(s) 24, 27, 30, 101, 104, 211, 489, 529
Sau3AI GATC 3 cut(s) 45, 629, 670
Sau96I GGNCC 2 cut(s) 188, 350
ScaI AGTACT 3 cut(s) 404, 652, 689
SchI GAGTC 3 cut(s) 168, 288, 602
SduI GDGCHC 2 cut(s) 147, 265
SetI ASST 7 cut(s) 147, 336, 348, 384, 464, 502, 510
SfaNI GCATC 2 cut(s) 483, 616
SfcI CTRYAG 1 cut(s) 406
Sfr274I CTCGAG 1 cut(s) 183
Sfr303I CCGCGG 1 cut(s) 104
SgrBI CCGCGG 1 cut(s) 104
SinI GGWCC 1 cut(s) 350
SlaI CTCGAG 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 712
SmlI CTYRAG 2 cut(s) 146, 183
SmoI CTYRAG 2 cut(s) 146, 183
SphI GCATGC 1 cut(s) 495
Sse9I AATT 2 cut(s) 554, 724
SsiI CCGC 3 cut(s) 101, 103, 211
SspMI CTAG 3 cut(s) 194, 296, 509
SstI GAGCTC 1 cut(s) 147
StyI CCWWGG 1 cut(s) 250
TaaI ACNGT 3 cut(s) 535, 621, 692
TaqI TCGA 3 cut(s) 88, 184, 396
TasI AATT 2 cut(s) 554, 724
TatI WGTACW 4 cut(s) 375, 402, 650, 687
TauI GCSGC 3 cut(s) 103, 106, 213
TfiI GAWTC 1 cut(s) 127
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TscAI CASTG 1 cut(s) 626
TseI GCWGC 5 cut(s) 23, 26, 29, 488, 528
TspDTI ATGAA 2 cut(s) 505, 629
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 1 cut(s) 626
VpaK11BI GGWCC 1 cut(s) 350
XagI CCTNNNNNAGG 1 cut(s) 338
XceI RCATGY 1 cut(s) 495
XhoI CTCGAG 1 cut(s) 183
XmiI GTMKAC 2 cut(s) 562, 696
XspI CTAG 3 cut(s) 194, 296, 509
ZrmI AGTACT 3 cut(s) 404, 652, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.