Rroxscaffold_152G00434520

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0010559
Physical Location & Seq
Forward (+)
64979 .. 65716
738 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434520.1

Sequence Viewer

Length: 738 bp
ATGGGGAAGGAAAACCTTATAATTGCAAGCAGATGGTTAATCTTGCAGAAGATAACCAGCACTTGCAGAAGGAGATTTCAAGAAAGTTTGGCGTGTACTACTTTTCCTACACTTGATGCAAAAATCTTAGCAAGAGGTTTCCATCAAGTTCGTGGTGGCAGCTCATCATTGACAGAAGAGCTGGAGGGTATTCTTAATCCAGAAGATCAAGGGAGGGAGAGTAGTACTAACTCTATGACCTGCATTTACAAGGTTCCTAGCACCATGCGTCAGGTGAACAGGAAGGCTTACGAGCCCAACATCGTGTCCATCGGTCCTTACCATCACGGAGTTGAAAGCCTACAAGAAATGGAGAAGCTTAAGCACACATACTTTCGGCGCCTCTTTCAACCAAACTACCATGATCGGTTGGATGATGAAGTAGTGTCGCTGAAGAAGAAGGCGGTAGATGATGCGAAGAAGGCAATGGAGGAATTGGAGGTGGCGGCTAGGAGCTGCTACTCCGAACAATCCAAGCTTAGCAGCAAGGCGTTTGTAAAGATGATGCTGATTGATGGTTGCTTCATTATAGGGTTGCTCAGGGACTCATTGCCACTACAAGAAAAAAGGCTTTTTGCAAGGAATTTTTTCCTTGTAAAAAGCTGGACATTCGTTGCATTAACCCAAATGCAAGGAATTTTCCTTGCATTTGAGTCCTTGCAAAGAAGCCCTTGCAAAAGAGCAAATACAACAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

245

Amino Acids

28.07

Weight (kDa)

9.3

Isoelectric Point (pI)

59.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 82 - 196 3.5e-34 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 20
Acc36I ACCTGC 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 378
AciI CCGC 2 cut(s) 443, 485
AcsI RAATTY 2 cut(s) 622, 675
AcuI CTGAAG 1 cut(s) 452
AcyI GRCGYC 1 cut(s) 379
AfaI GTAC 2 cut(s) 97, 226
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 3 cut(s) 80, 335, 389
AluBI AGCT 6 cut(s) 162, 181, 358, 495, 517, 642
AluI AGCT 6 cut(s) 162, 181, 358, 495, 517, 642
ApeKI GCWGC 3 cut(s) 159, 495, 522
ApoI RAATTY 2 cut(s) 622, 675
Asp700I GAANNNNTTC 1 cut(s) 626
AspLEI GCGC 1 cut(s) 381
AspS9I GGNCC 1 cut(s) 314
AsuHPI GGTGA 1 cut(s) 286
AvaII GGWCC 1 cut(s) 314
BanI GGYRCC 1 cut(s) 378
BanII GRGCYC 1 cut(s) 297
BbvI GCAGC 3 cut(s) 171, 482, 534
BccI CCATC 5 cut(s) 27, 150, 317, 330, 548
BfaI CTAG 2 cut(s) 258, 489
BfoI RGCGCY 1 cut(s) 382
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 4 cut(s) 160, 486, 496, 523
BlpI GCTNAGC 1 cut(s) 518
BlsI GCNGC 4 cut(s) 161, 487, 497, 524
BmcAI AGTACT 1 cut(s) 226
Bme18I GGWCC 1 cut(s) 314
BmgT120I GGNCC 1 cut(s) 314
BmiI GGNNCC 2 cut(s) 255, 380
BmsI GCATC 3 cut(s) 106, 442, 534
BpmI CTGGAG 1 cut(s) 203
Bpu10I CCTNAGC 1 cut(s) 578
Bpu1102I GCTNAGC 1 cut(s) 518
BsaHI GRCGYC 1 cut(s) 379
BsaXI ACNNNNNCTCC 2 cut(s) 205, 235
Bse3DI GCAATG 2 cut(s) 471, 587
BseGI GGATG 1 cut(s) 418
BseMI GCAATG 2 cut(s) 471, 587
BseMII CTCAG 1 cut(s) 592
BseXI GCAGC 3 cut(s) 171, 482, 534
BshNI GGYRCC 1 cut(s) 378
BslFI GGGAC 1 cut(s) 596
BsmFI GGGAC 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 205, 403
Bsp1720I GCTNAGC 1 cut(s) 518
BspACI CCGC 2 cut(s) 443, 485
BspCNI CTCAG 1 cut(s) 591
BspLI GGNNCC 2 cut(s) 255, 380
BspMI ACCTGC 1 cut(s) 248
BspQI GCTCTTC 1 cut(s) 171
BspT107I GGYRCC 1 cut(s) 378
BspTI CTTAAG 1 cut(s) 359
BsrDI GCAATG 2 cut(s) 471, 587
BssMI GATC 2 cut(s) 205, 403
BssNI GRCGYC 1 cut(s) 379
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 379
BstAFI CTTAAG 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 3 cut(s) 127, 518, 578
BstF5I GGATG 1 cut(s) 418
BstH2I RGCGCY 1 cut(s) 382
BstHHI GCGC 1 cut(s) 381
BstKTI GATC 2 cut(s) 208, 406
BstMBI GATC 2 cut(s) 205, 403
BstMWI GCNNNNNNNGC 1 cut(s) 461
BstV1I GCAGC 3 cut(s) 171, 482, 534
BtsCI GGATG 1 cut(s) 418
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 1 cut(s) 28
CfoI GCGC 1 cut(s) 381
Cfr13I GGNCC 1 cut(s) 314
CseI GACGC 1 cut(s) 257
Csp6I GTAC 2 cut(s) 96, 225
CviAII CATG 2 cut(s) 265, 401
CviQI GTAC 2 cut(s) 96, 225
DdeI CTNAG 3 cut(s) 127, 518, 578
DinI GGCGCC 1 cut(s) 380
DpnI GATC 2 cut(s) 207, 405
DpnII GATC 2 cut(s) 205, 403
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
Eco24I GRGCYC 1 cut(s) 297
Eco47I GGWCC 1 cut(s) 314
Eco57I CTGAAG 1 cut(s) 452
EcoT38I GRGCYC 1 cut(s) 297
EgeI GGCGCC 1 cut(s) 380
EheI GGCGCC 1 cut(s) 380
FaeI CATG 2 cut(s) 268, 404
FaiI YATR 6 cut(s) 20, 236, 266, 370, 402, 569
FalI AAGNNNNNCTT 2 cut(s) 694, 726
FaqI GGGAC 1 cut(s) 596
FatI CATG 2 cut(s) 264, 400
Fnu4HI GCNGC 4 cut(s) 160, 486, 496, 523
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 297
Fsp4HI GCNGC 4 cut(s) 160, 486, 496, 523
FspBI CTAG 2 cut(s) 258, 489
GlaI GCGC 1 cut(s) 380
GluI GCNGC 4 cut(s) 160, 486, 496, 523
GsuI CTGGAG 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 382
HgaI GACGC 1 cut(s) 257
HhaI GCGC 1 cut(s) 381
Hin1I GRCGYC 1 cut(s) 379
Hin1II CATG 2 cut(s) 268, 404
Hin6I GCGC 1 cut(s) 379
HinP1I GCGC 1 cut(s) 379
HindIII AAGCTT 2 cut(s) 356, 515
HinfI GANTC 2 cut(s) 584, 692
HphI GGTGA 1 cut(s) 286
Hpy166II GTNNAC 2 cut(s) 96, 277
Hpy188I TCNGA 1 cut(s) 505
Hpy188III TCNNGA 2 cut(s) 80, 200
Hpy8I GTNNAC 2 cut(s) 96, 277
HpyAV CCTTC 4 cut(s) 63, 277, 433, 454
HpyF10VI GCNNNNNNNGC 1 cut(s) 461
HpyF3I CTNAG 3 cut(s) 127, 518, 578
Hsp92I GRCGYC 1 cut(s) 379
Hsp92II CATG 2 cut(s) 268, 404
HspAI GCGC 1 cut(s) 379
KasI GGCGCC 1 cut(s) 378
Kzo9I GATC 2 cut(s) 205, 403
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 1 cut(s) 492
LpnPI CCDG 8 cut(s) 70, 167, 213, 253, 257, 265, 565, 628
Lsp1109I GCAGC 3 cut(s) 171, 482, 534
LweI GCATC 3 cut(s) 106, 442, 534
MaeI CTAG 2 cut(s) 258, 489
MalI GATC 2 cut(s) 207, 405
MboI GATC 2 cut(s) 205, 403
MboII GAAGA 6 cut(s) 61, 188, 215, 445, 448, 469
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 4 cut(s) 21, 473, 622, 675
Mly113I GGCGCC 1 cut(s) 379
MlyI GAGTC 2 cut(s) 578, 701
MmeI TCCRAC 1 cut(s) 390
MnlI CCTC 6 cut(s) 128, 178, 207, 392, 463, 472
MroXI GAANNNNTTC 1 cut(s) 626
MseI TTAA 4 cut(s) 38, 195, 360, 659
MspCI CTTAAG 1 cut(s) 359
MwoI GCNNNNNNNGC 1 cut(s) 461
NarI GGCGCC 1 cut(s) 379
NdeII GATC 2 cut(s) 205, 403
NlaIII CATG 2 cut(s) 268, 404
NlaIV GGNNCC 2 cut(s) 255, 380
PciSI GCTCTTC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 626
PkrI GCNGC 4 cut(s) 161, 487, 497, 524
PleI GAGTC 2 cut(s) 578, 700
PluTI GGCGCC 1 cut(s) 382
PpsI GAGTC 2 cut(s) 578, 700
PsiI TTATAA 1 cut(s) 20
PspN4I GGNNCC 2 cut(s) 255, 380
PspPI GGNCC 1 cut(s) 314
RsaI GTAC 2 cut(s) 97, 226
RsaNI GTAC 2 cut(s) 96, 225
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 4 cut(s) 38, 195, 360, 659
SatI GCNGC 4 cut(s) 160, 486, 496, 523
Sau3AI GATC 2 cut(s) 205, 403
Sau96I GGNCC 1 cut(s) 314
ScaI AGTACT 1 cut(s) 226
SchI GAGTC 2 cut(s) 578, 701
SduI GDGCHC 1 cut(s) 297
SfaNI GCATC 3 cut(s) 106, 442, 534
SfoI GGCGCC 1 cut(s) 380
SinI GGWCC 1 cut(s) 314
SmlI CTYRAG 1 cut(s) 359
SmoI CTYRAG 1 cut(s) 359
Sse9I AATT 4 cut(s) 21, 473, 622, 675
SsiI CCGC 2 cut(s) 443, 485
SspDI GGCGCC 1 cut(s) 378
SspMI CTAG 2 cut(s) 258, 489
TaqII GACCGA 1 cut(s) 302
TasI AATT 4 cut(s) 21, 473, 622, 675
TatI WGTACW 2 cut(s) 95, 224
TauI GCSGC 1 cut(s) 488
Tru1I TTAA 4 cut(s) 38, 195, 360, 659
Tru9I TTAA 4 cut(s) 38, 195, 360, 659
TseI GCWGC 3 cut(s) 159, 495, 522
TspDTI ATGAA 2 cut(s) 432, 553
TspGWI ACGGA 1 cut(s) 342
Vha464I CTTAAG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 314
XapI RAATTY 2 cut(s) 622, 675
XcmI CCANNNNNNNNNTGG 1 cut(s) 149
XmnI GAANNNNTTC 1 cut(s) 626
XspI CTAG 2 cut(s) 258, 489
ZrmI AGTACT 1 cut(s) 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.