MD04G1048600.v1.1

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0010559
Physical Location & Seq
Forward (+)
5705336 .. 5706061
726 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1048600.v1.1.491

Sequence Viewer

Length: 726 bp
ATGAATCGTCAGCCATTAGATATAAGTTTCAGAAGCACGCTGAGGGGAAAGGTTCTGACTATCCCTCAAATACACATCAATGATCATAGAGGTACTTTGTTTCGTAACCTGCTGGCATTTGAAAAATGTCACCGGAATTGCCATCAAGATGTTACAAGCTATCTGTTTTTTCTCGACGGTCTGATCAATTCATCCAAGGATGTAGGGCTTCTCCACTATCATGGAGTCCTCCAACATTCTCTAGGCAGCAACAGAAGCGTGGCGAAACTTGTCAACAACCTCTGCAAGGAAGTTGGTCCGGATGCATCCCAGACATACTTATACAGGGTGATTGGCGATGCCAACTTCTACTATGATTCTAAACTTGCAAAAGTAAGAGCAGCATTGGTGCACCACTATTTAAGTAGCTGGGTTGTGGGCATCTCAACGCTCGGTGGAATCTTAGCTCTTTACCTCACCTTGATTCAGACCGGCTGTGGAGTTGCCGCTGCCAAAAAGGATTTGGAGCACAAGTTCAGTTTCGGAGCCTTTCTCAAGGATTCGTTGTTCATAACATATCTGGAAAAGATTCTAGTCTTCAGTAAGGATTTGGAGCACAAGTTCTATGTCGGAGCCTTTCTCACTGACGAGGACAGTGACAAGGACAGTGACGAGGACAATGACGAGGCCCTTAATGCCATGGACTGGCCACATGATTTGGAACAATTTATGGAATCAATGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

242

Amino Acids

27.33

Weight (kDa)

6.03

Isoelectric Point (pI)

35.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 3 - 152 5.1e-41 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 117
AccB7I CCANNNNNTGG 1 cut(s) 684
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 1 cut(s) 486
AcoI YGGCCR 1 cut(s) 686
AcuI CTGAAG 1 cut(s) 562
AfaI GTAC 1 cut(s) 94
AfiI CCNNNNNNNGG 2 cut(s) 286, 684
AgsI TTSAA 1 cut(s) 122
AloI GAACNNNNNNTCC 6 cut(s) 497, 529, 530, 562, 584, 616
AluBI AGCT 3 cut(s) 159, 408, 446
AluI AGCT 3 cut(s) 159, 408, 446
Alw21I GWGCWC 3 cut(s) 393, 510, 597
Alw44I GTGCAC 1 cut(s) 389
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 2 cut(s) 666, 686
ApaLI GTGCAC 1 cut(s) 389
ApeKI GCWGC 3 cut(s) 246, 380, 488
Asp700I GAANNNNTTC 1 cut(s) 567
AspS9I GGNCC 2 cut(s) 296, 667
AsuHPI GGTGA 3 cut(s) 122, 340, 448
AvaII GGWCC 1 cut(s) 296
BaeGI GKGCMC 1 cut(s) 393
BalI TGGCCA 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 568
Bbv12I GWGCWC 3 cut(s) 393, 510, 597
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 3 cut(s) 258, 392, 475
BccI CCATC 1 cut(s) 150
BclI TGATCA 2 cut(s) 82, 183
BfaI CTAG 2 cut(s) 242, 572
BfuAI ACCTGC 1 cut(s) 117
BisI GCNGC 4 cut(s) 247, 381, 486, 489
BlsI GCNGC 4 cut(s) 248, 382, 487, 490
Bme18I GGWCC 1 cut(s) 296
BmgT120I GGNCC 2 cut(s) 296, 667
BmiI GGNNCC 2 cut(s) 526, 613
BmsI GCATC 4 cut(s) 292, 314, 328, 429
BpiI GAAGAC 1 cut(s) 568
BplI GAGNNNNNCTC 4 cut(s) 516, 548, 603, 635
Bpu10I CCTNAGC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 518
BsaJI CCNNGG 2 cut(s) 195, 678
BsaWI WCCGGW 2 cut(s) 132, 298
BsaXI ACNNNNNCTCC 4 cut(s) 497, 527, 584, 614
Bsc4I CCNNNNNNNGG 2 cut(s) 286, 684
Bse118I RCCGGY 1 cut(s) 470
Bse1I ACTGG 1 cut(s) 689
BseAI TCCGGA 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 195, 678
BseGI GGATG 4 cut(s) 191, 205, 305, 307
BseLI CCNNNNNNNGG 2 cut(s) 286, 684
BseMII CTCAG 1 cut(s) 32
BseNI ACTGG 1 cut(s) 689
BseSI GKGCMC 1 cut(s) 393
BseXI GCAGC 3 cut(s) 258, 392, 475
BseYI CCCAGC 1 cut(s) 408
BshFI GGCC 2 cut(s) 668, 688
BsiHKAI GWGCWC 3 cut(s) 393, 510, 597
BsiSI CCGG 3 cut(s) 133, 299, 471
BslI CCNNNNNNNGG 2 cut(s) 286, 684
BsnI GGCC 2 cut(s) 668, 688
Bsp1286I GDGCHC 3 cut(s) 393, 510, 597
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 2 cut(s) 82, 183
Bsp19I CCATGG 1 cut(s) 678
BspACI CCGC 1 cut(s) 486
BspANI GGCC 2 cut(s) 668, 688
BspCNI CTCAG 1 cut(s) 33
BspEI TCCGGA 1 cut(s) 298
BspLI GGNNCC 2 cut(s) 526, 613
BspMI ACCTGC 1 cut(s) 117
BsrFI RCCGGY 1 cut(s) 470
BsrI ACTGG 1 cut(s) 689
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 195, 678
BssMI GATC 2 cut(s) 82, 183
BssT1I CCWWGG 2 cut(s) 195, 678
Bst4CI ACNGT 3 cut(s) 179, 635, 647
BstC8I GCNNGC 2 cut(s) 38, 114
BstDEI CTNAG 2 cut(s) 41, 442
BstDSI CCRYGG 1 cut(s) 678
BstENI CCTNNNNNAGG 1 cut(s) 284
BstF5I GGATG 4 cut(s) 191, 205, 305, 307
BstKTI GATC 2 cut(s) 85, 186
BstMBI GATC 2 cut(s) 82, 183
BstMWI GCNNNNNNNGC 2 cut(s) 255, 674
BstSLI GKGCMC 1 cut(s) 393
BstV1I GCAGC 3 cut(s) 258, 392, 475
BstV2I GAAGAC 1 cut(s) 568
BstXI CCANNNNNNTGG 1 cut(s) 221
BsuRI GGCC 2 cut(s) 668, 688
BtgI CCRYGG 1 cut(s) 678
BtgZI GCGATG 1 cut(s) 351
BtsCI GGATG 4 cut(s) 191, 205, 305, 307
BtsIMutI CAGTG 3 cut(s) 621, 640, 652
BveI ACCTGC 1 cut(s) 117
Cac8I GCNNGC 2 cut(s) 38, 114
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 2 cut(s) 296, 667
Csp6I GTAC 1 cut(s) 93
CspCI CAANNNNNGTGG 2 cut(s) 678, 713
CviAII CATG 3 cut(s) 221, 679, 692
CviQI GTAC 1 cut(s) 93
DdeI CTNAG 2 cut(s) 41, 442
DpnI GATC 2 cut(s) 84, 185
DpnII GATC 2 cut(s) 82, 183
EaeI YGGCCR 1 cut(s) 686
Eco130I CCWWGG 2 cut(s) 195, 678
Eco47I GGWCC 1 cut(s) 296
Eco57I CTGAAG 1 cut(s) 562
EcoNI CCTNNNNNAGG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 667
EcoT14I CCWWGG 2 cut(s) 195, 678
EcoT22I ATGCAT 1 cut(s) 307
ErhI CCWWGG 2 cut(s) 195, 678
FaeI CATG 3 cut(s) 224, 682, 695
FatI CATG 3 cut(s) 220, 678, 691
FbaI TGATCA 2 cut(s) 82, 183
Fnu4HI GCNGC 4 cut(s) 247, 381, 486, 489
FokI GGATG 4 cut(s) 178, 212, 292, 314
Fsp4HI GCNGC 4 cut(s) 247, 381, 486, 489
FspBI CTAG 2 cut(s) 242, 572
GluI GCNGC 4 cut(s) 247, 381, 486, 489
GsaI CCCAGC 1 cut(s) 412
HaeIII GGCC 2 cut(s) 668, 688
HapII CCGG 3 cut(s) 133, 299, 471
Hin1II CATG 3 cut(s) 224, 682, 695
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HinfI GANTC 8 cut(s) 4, 225, 356, 438, 463, 539, 568, 713
HpaII CCGG 3 cut(s) 133, 299, 471
HphI GGTGA 3 cut(s) 122, 340, 448
Hpy166II GTNNAC 2 cut(s) 274, 391
Hpy188I TCNGA 6 cut(s) 32, 57, 183, 468, 524, 611
Hpy188III TCNNGA 4 cut(s) 146, 173, 299, 560
Hpy8I GTNNAC 2 cut(s) 274, 391
Hpy99I CGWCG 1 cut(s) 179
HpyCH4III ACNGT 3 cut(s) 179, 635, 647
HpyCH4V TGCA 4 cut(s) 285, 305, 368, 391
HpyF10VI GCNNNNNNNGC 2 cut(s) 255, 674
HpyF3I CTNAG 2 cut(s) 41, 442
Hsp92II CATG 3 cut(s) 224, 682, 695
Kpn2I TCCGGA 1 cut(s) 298
Ksp22I TGATCA 2 cut(s) 82, 183
Kzo9I GATC 2 cut(s) 82, 183
LmnI GCTCC 4 cut(s) 505, 524, 592, 611
Lsp1109I GCAGC 3 cut(s) 258, 392, 475
LweI GCATC 4 cut(s) 292, 314, 328, 429
MaeI CTAG 2 cut(s) 242, 572
MaeIII GTNAC 5 cut(s) 104, 128, 151, 635, 647
MalI GATC 2 cut(s) 84, 185
MboI GATC 2 cut(s) 82, 183
MboII GAAGA 1 cut(s) 568
MhlI GDGCHC 3 cut(s) 393, 510, 597
MlsI TGGCCA 1 cut(s) 688
MluCI AATT 3 cut(s) 136, 187, 704
MluNI TGGCCA 1 cut(s) 688
MlyI GAGTC 1 cut(s) 234
MmeI TCCRAC 2 cut(s) 256, 589
MnlI CCTC 9 cut(s) 36, 75, 83, 239, 290, 464, 622, 646, 658
Mox20I TGGCCA 1 cut(s) 688
Mph1103I ATGCAT 1 cut(s) 307
MroI TCCGGA 1 cut(s) 298
MroXI GAANNNNTTC 1 cut(s) 567
MscI TGGCCA 1 cut(s) 688
MseI TTAA 3 cut(s) 401, 672, 724
MslI CAYNNNNRTG 3 cut(s) 78, 147, 219
Msp20I TGGCCA 1 cut(s) 688
MspA1I CMGCKG 1 cut(s) 488
MspI CCGG 3 cut(s) 133, 299, 471
MwoI GCNNNNNNNGC 2 cut(s) 255, 674
NcoI CCATGG 1 cut(s) 678
NdeII GATC 2 cut(s) 82, 183
NlaIII CATG 3 cut(s) 224, 682, 695
NlaIV GGNNCC 2 cut(s) 526, 613
NmuCI GTSAC 3 cut(s) 128, 635, 647
NsiI ATGCAT 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 7 cut(s) 4, 356, 438, 463, 539, 568, 713
PflMI CCANNNNNTGG 1 cut(s) 684
PkrI GCNGC 4 cut(s) 248, 382, 487, 490
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PspFI CCCAGC 1 cut(s) 408
PspN4I GGNNCC 2 cut(s) 526, 613
PspPI GGNCC 2 cut(s) 296, 667
RsaI GTAC 1 cut(s) 94
RsaNI GTAC 1 cut(s) 93
RseI CAYNNNNRTG 3 cut(s) 78, 147, 219
SaqAI TTAA 3 cut(s) 401, 672, 724
SatI GCNGC 4 cut(s) 247, 381, 486, 489
Sau3AI GATC 2 cut(s) 82, 183
Sau96I GGNCC 2 cut(s) 296, 667
SchI GAGTC 1 cut(s) 234
SduI GDGCHC 3 cut(s) 393, 510, 597
SetI ASST 9 cut(s) 54, 94, 111, 161, 282, 410, 448, 456, 461
SfaNI GCATC 4 cut(s) 292, 314, 328, 429
SinI GGWCC 1 cut(s) 296
SmiMI CAYNNNNRTG 3 cut(s) 78, 147, 219
SmlI CTYRAG 1 cut(s) 533
SmoI CTYRAG 1 cut(s) 533
Sse9I AATT 3 cut(s) 136, 187, 704
SsiI CCGC 1 cut(s) 486
SspMI CTAG 2 cut(s) 242, 572
StyI CCWWGG 2 cut(s) 195, 678
TaaI ACNGT 3 cut(s) 179, 635, 647
TaqI TCGA 1 cut(s) 174
TasI AATT 3 cut(s) 136, 187, 704
TauI GCSGC 1 cut(s) 488
TfiI GAWTC 7 cut(s) 4, 356, 438, 463, 539, 568, 713
Tru1I TTAA 3 cut(s) 401, 672, 724
Tru9I TTAA 3 cut(s) 401, 672, 724
TscAI CASTG 3 cut(s) 628, 640, 652
TseFI GTSAC 3 cut(s) 128, 635, 647
TseI GCWGC 3 cut(s) 246, 380, 488
Tsp45I GTSAC 3 cut(s) 128, 635, 647
TspDTI ATGAA 3 cut(s) 17, 180, 538
TspRI CASTG 3 cut(s) 628, 640, 652
Van91I CCANNNNNTGG 1 cut(s) 684
VneI GTGCAC 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 296
XagI CCTNNNNNAGG 1 cut(s) 284
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 2 cut(s) 242, 572
Zsp2I ATGCAT 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.