RLG00000020145

Plant protein of unknown function

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0010559
Physical Location & Seq
Forward (+)
61618724 .. 61622250
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020145

Sequence Viewer

Length: 1962 bp
ATGGGGAAGGGAAACCTTATAATTGCAAGCAGATGGTTAATCTTGCAGAAGATAACCAGCACTTGCAGAAGGAGATTTCAAGAAAGTTTGGCTTGTACTACTTTTCCTACACTTGATGCAAAAATCTTAGCAAGAGGTTTCCATCAAGTTGGTGGTGGCAGCTCATCATTGACAGAAGAGCTGGAGGGTATTCTTAATCCTGAAGATCAAGGGAGGGAGAGTAGTACTAACTCTATGACCTGCATTTACAAGGTTCCTAGCACCATGCGTCAGGTGAACAGGAAGGCTTACGAGCCCAACATTGTGTCCATCGGTCCTTACCATCACGGAGTTGAAAGCCTACAAGAAATGGAGAAGCTTAAGCACACATACTTTCGGCGCCTCTTTCAACCAAACTACCATGATCGGTTGGATGATGAAGTAGTGTCGCTGAAGAAGAAGGCCGTAGATGATGCGAAGAAGGCAATGGAGGAATTGGAGGTGGCGGCTAGGAGCTGCTACTCCGAACAATCCAAGCTTAGCAGCAAGGCGTTTGTAAAGATGATGCTGATTGATGGTTGCTTCATTATAGGGTTGCTCAGGGACTCATTGCAGTCCGATTTTGAGCACACACCCTCCATCATTCAAAGGTGGATGCTTCCAACTCTGCGCCGGGATTTGATCAAAATCGAAAATCAGCTTCCCCTCTTTGTCTTGCGCAAATTATTTCAGCTGACCGAAGCAAGCACTACTTGTCATGAGAAACCAATTGCAAGTTGGAAATTGGAAGCACTAGCCCTTCGCTTCTTTATGCCGCTACTGCGAGGAGATGATGTTCTGACCAAACCACCGCAGCATTATGCACCACAACATTGTCAAAGCAGCAGCAGCAAGCACTTTCTTGATCTTTTCCACAGGACAATCAGTCCGGACTGCTCTACCCGTTCGGTACGGCAAGCGCCGCGGCGAGGGAATCAAACCAAGCAGATTCTATCAGTACAAGAGCTTAAGGAGGCCGGAGTCAAGTTCAGAACAGACCGCACTCAAGGGCCACTAGACATCACTTTCGGCCGCGTCGCTTGGAGGTGGGGGAAGGTGCTCACAATCCCTCGAATACACATTGATGATCACAGAGGCACATTGTTTCGAAACATGGTTGCATTCGAAAAATGTGACCCCAACTGCCACCAAGATGTTACAACCTATTTGTTTTTCCTCGACAGCCTCATCAACTCGGCCAAGGATGTAGGGCTCCTGCACTACCACGGAGTCCTGCACCACTCTCTAGGAAGCAACAGAAGGGTGGCGAAACTCGTCAACAACCTTTGCAAGGAGGTTGGTCCGGATGTTTCCAAGTCCTACTTGTACAAGGTGGTTAGAGATGTCAATGAGTACTATAGTTCCGAGTATGGAAAAATAAGAGGCTTCTTGGTACACCACCATTTTAGTAGCTGGTTGGTGGGCATCTCAACTCTTGCAGCATGCTTAGCTCTTTACCTAGCACTCATTCAGACCGGCTGCACAGTCGCCACTGCCAGAGAAGCCTTGCATAAACACACCACATTTGGAGACTTTCTGAAAGAGTCTTTGCTTTTGCATCATATGAAAGAGTGGTGGGGATCATTTGGAAATACAGGCAGCACTAATCGCAATGACATGGAGTATTGTCCTCGGCGACGGCATGAACATCTCCCACCACCATGCGCACATCTTCTACGACCTCACCGGCCGTTGCTTCGCGCTGGAGGTCATCAAGAAGAATGGCTGCCTCATGGAGGGGATTGTCCACCTCCCCAGAAACCTTCTGTCAAGTTCGATTCACAGGATCTGCTTCAGATTGGGGATAAAGGGTTTTATTTTGTGTTGCCGATAAGGATTACTCTGGGTGGACCTATTGAGCCCAGGCATTACGCGATGGCGGTCAGGGGAGGTGGTGGTAGTTCCTATCTAGCTTTCTGTAGAGATGATAATATGATGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

654

Amino Acids

74.41

Weight (kDa)

9.11

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 82 - 493 1.1e-109 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 20
Acc16I TGCGCA 2 cut(s) 698, 1684
Acc36I ACCTGC 1 cut(s) 248
AccB1I GGYRCC 1 cut(s) 378
AccII CGCG 4 cut(s) 943, 1053, 1719, 1892
AccIII TCCGGA 2 cut(s) 907, 1321
AciI CCGC 8 cut(s) 485, 794, 830, 941, 943, 1018, 1051, 1898
AclWI GGATC 2 cut(s) 1606, 1812
AcoI YGGCCR 3 cut(s) 1048, 1215, 1706
AcuI CTGAAG 3 cut(s) 222, 452, 1796
AcyI GRCGYC 1 cut(s) 379
AfaI GTAC 7 cut(s) 97, 226, 930, 978, 1346, 1373, 1413
AfiI CCNNNNNNNGG 3 cut(s) 947, 1309, 1754
AflII CTTAAG 2 cut(s) 359, 986
AgsI TTSAA 4 cut(s) 80, 335, 389, 626
AhdI GACNNNNNGTC 1 cut(s) 903
AjnI CCWGG 1 cut(s) 1880
AloI GAACNNNNNNTCC 2 cut(s) 798, 830
Alw21I GWGCWC 2 cut(s) 609, 1080
Alw26I GTCTC 1 cut(s) 1542
AlwI GGATC 2 cut(s) 1606, 1812
AlwNI CAGNNNCTG 1 cut(s) 1807
Aor13HI TCCGGA 2 cut(s) 907, 1321
AoxI GGCC 6 cut(s) 441, 993, 1028, 1048, 1215, 1706
ArsI GACNNNNNNTTYG 2 cut(s) 1028, 1060
AspLEI GCGC 6 cut(s) 381, 651, 699, 940, 1685, 1721
AspS9I GGNCC 4 cut(s) 314, 1028, 1319, 1868
AsuC2I CCSGG 1 cut(s) 653
AsuHPI GGTGA 2 cut(s) 286, 1694
AsuII TTCGAA 2 cut(s) 1126, 1143
AvaII GGWCC 3 cut(s) 314, 1319, 1868
BanI GGYRCC 1 cut(s) 378
BanII GRGCYC 3 cut(s) 297, 1233, 1881
Bbv12I GWGCWC 2 cut(s) 609, 1080
BccI CCATC 8 cut(s) 27, 150, 317, 330, 548, 626, 1888, 1948
BceAI ACGGC 4 cut(s) 428, 947, 1673, 1693
BciT130I CCWGG 1 cut(s) 1882
BclI TGATCA 2 cut(s) 660, 1105
BcnI CCSGG 1 cut(s) 653
BcoDI GTCTC 1 cut(s) 1542
BfaI CTAG 7 cut(s) 258, 489, 773, 1034, 1265, 1478, 1928
BfmI CTRYAG 2 cut(s) 1375, 1936
BfoI RGCGCY 2 cut(s) 382, 941
BfrI CTTAAG 2 cut(s) 359, 986
BfuAI ACCTGC 1 cut(s) 248
BlpI GCTNAGC 2 cut(s) 518, 1465
BmcAI AGTACT 2 cut(s) 226, 1373
Bme1390I CCNGG 2 cut(s) 653, 1882
Bme18I GGWCC 3 cut(s) 314, 1319, 1868
BmeRI GACNNNNNGTC 1 cut(s) 903
BmgT120I GGNCC 4 cut(s) 314, 1028, 1319, 1868
BmiI GGNNCC 3 cut(s) 255, 380, 1232
BmrFI CCNGG 2 cut(s) 653, 1882
BmsI GCATC 6 cut(s) 106, 442, 534, 624, 1452, 1585
BpmI CTGGAG 2 cut(s) 203, 1743
Bpu10I CCTNAGC 1 cut(s) 578
Bpu1102I GCTNAGC 2 cut(s) 518, 1465
Bpu14I TTCGAA 2 cut(s) 1126, 1143
BpuEI CTTGAG 1 cut(s) 1008
BpuMI CCSGG 1 cut(s) 653
BsaBI GATNNNNATC 1 cut(s) 665
BsaHI GRCGYC 1 cut(s) 379
BsaJI CCNNGG 5 cut(s) 941, 1218, 1243, 1649, 1880
BsaWI WCCGGW 2 cut(s) 907, 1321
BsaXI ACNNNNNCTCC 6 cut(s) 205, 235, 599, 629, 798, 828
Bsc4I CCNNNNNNNGG 3 cut(s) 947, 1309, 1754
Bse118I RCCGGY 2 cut(s) 1493, 1704
Bse3DI GCAATG 3 cut(s) 471, 587, 1636
Bse8I GATNNNNATC 1 cut(s) 665
BseAI TCCGGA 2 cut(s) 907, 1321
BseBI CCWGG 1 cut(s) 1882
BseDI CCNNGG 5 cut(s) 941, 1218, 1243, 1649, 1880
BseGI GGATG 4 cut(s) 418, 639, 1228, 1330
BseJI GATNNNNATC 1 cut(s) 665
BseLI CCNNNNNNNGG 3 cut(s) 947, 1309, 1754
BseMI GCAATG 3 cut(s) 471, 587, 1636
BseMII CTCAG 1 cut(s) 592
BseRI GAGGAG 1 cut(s) 819
BseX3I CGGCCG 2 cut(s) 1048, 1706
BsgI GTGCAG 3 cut(s) 1220, 1238, 1483
Bsh1236I CGCG 4 cut(s) 943, 1053, 1719, 1892
Bsh1285I CGRYCG 2 cut(s) 1051, 1709
BshFI GGCC 6 cut(s) 443, 995, 1030, 1050, 1217, 1708
BshNI GGYRCC 1 cut(s) 378
BsiEI CGRYCG 2 cut(s) 1051, 1709
BsiHKAI GWGCWC 2 cut(s) 609, 1080
BsiSI CCGG 6 cut(s) 652, 908, 996, 1322, 1494, 1705
BslFI GGGAC 1 cut(s) 596
BslI CCNNNNNNNGG 3 cut(s) 947, 1309, 1754
BsmAI GTCTC 1 cut(s) 1542
BsmFI GGGAC 1 cut(s) 596
BsmI GAATGC 1 cut(s) 1139
BsnI GGCC 6 cut(s) 443, 995, 1030, 1050, 1217, 1708
Bsp119I TTCGAA 2 cut(s) 1126, 1143
Bsp1286I GDGCHC 5 cut(s) 297, 609, 1080, 1233, 1881
Bsp13I TCCGGA 2 cut(s) 907, 1321
Bsp1407I TGTACA 1 cut(s) 1344
Bsp143I GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
Bsp1720I GCTNAGC 2 cut(s) 518, 1465
BspACI CCGC 8 cut(s) 485, 794, 830, 941, 943, 1018, 1051, 1898
BspANI GGCC 6 cut(s) 443, 995, 1030, 1050, 1217, 1708
BspCNI CTCAG 1 cut(s) 591
BspEI TCCGGA 2 cut(s) 907, 1321
BspFNI CGCG 4 cut(s) 943, 1053, 1719, 1892
BspHI TCATGA 1 cut(s) 736
BspLI GGNNCC 3 cut(s) 255, 380, 1232
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 1606, 1812
BspQI GCTCTTC 1 cut(s) 171
BspT104I TTCGAA 2 cut(s) 1126, 1143
BspT107I GGYRCC 1 cut(s) 378
BspTI CTTAAG 2 cut(s) 359, 986
BsrDI GCAATG 3 cut(s) 471, 587, 1636
BsrFI RCCGGY 2 cut(s) 1493, 1704
BsrGI TGTACA 1 cut(s) 1344
BssAI RCCGGY 2 cut(s) 1493, 1704
BssECI CCNNGG 5 cut(s) 941, 1218, 1243, 1649, 1880
BssMI GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
BssNI GRCGYC 1 cut(s) 379
BssT1I CCWWGG 1 cut(s) 1218
Bst2UI CCWGG 1 cut(s) 1882
Bst4CI ACNGT 1 cut(s) 1504
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 1 cut(s) 379
BstAFI CTTAAG 2 cut(s) 359, 986
BstAUI TGTACA 1 cut(s) 1344
BstBI TTCGAA 2 cut(s) 1126, 1143
BstC8I GCNNGC 5 cut(s) 28, 724, 872, 936, 1462
BstDEI CTNAG 4 cut(s) 127, 518, 578, 1465
BstDSI CCRYGG 2 cut(s) 941, 1243
BstENI CCTNNNNNAGG 2 cut(s) 1307, 1752
BstF5I GGATG 4 cut(s) 418, 639, 1228, 1330
BstFNI CGCG 4 cut(s) 943, 1053, 1719, 1892
BstH2I RGCGCY 2 cut(s) 382, 941
BstHHI GCGC 6 cut(s) 381, 651, 699, 940, 1685, 1721
BstKTI GATC 7 cut(s) 208, 406, 663, 886, 1108, 1601, 1807
BstMAI GTCTC 1 cut(s) 1542
BstMBI GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
BstMCI CGRYCG 2 cut(s) 1051, 1709
BstMWI GCNNNNNNNGC 7 cut(s) 461, 799, 867, 940, 1466, 1520, 1626
BstNI CCWGG 1 cut(s) 1882
BstNSI RCATGY 1 cut(s) 1464
BstSCI CCNGG 2 cut(s) 651, 1880
BstSFI CTRYAG 2 cut(s) 1375, 1936
BstUI CGCG 4 cut(s) 943, 1053, 1719, 1892
BstX2I RGATCY 1 cut(s) 1804
BstXI CCANNNNNNTGG 1 cut(s) 149
BstYI RGATCY 1 cut(s) 1804
BstZI CGGCCG 2 cut(s) 1048, 1706
BsuRI GGCC 6 cut(s) 443, 995, 1030, 1050, 1217, 1708
BtgI CCRYGG 2 cut(s) 941, 1243
BtgZI GCGATG 1 cut(s) 1907
BtsCI GGATG 4 cut(s) 418, 639, 1228, 1330
BtsI GCAGTG 1 cut(s) 1509
BtsIMutI CAGTG 1 cut(s) 1509
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 5 cut(s) 28, 724, 872, 936, 1462
CaiI CAGNNNCTG 1 cut(s) 1807
CciI TCATGA 1 cut(s) 736
CfoI GCGC 6 cut(s) 381, 651, 699, 940, 1685, 1721
Cfr10I RCCGGY 2 cut(s) 1493, 1704
Cfr13I GGNCC 4 cut(s) 314, 1028, 1319, 1868
Cfr42I CCGCGG 1 cut(s) 944
CseI GACGC 2 cut(s) 257, 1042
Csp6I GTAC 7 cut(s) 96, 225, 929, 977, 1345, 1372, 1412
CviAII CATG 9 cut(s) 265, 401, 737, 1132, 1461, 1636, 1661, 1680, 1751
CviQI GTAC 7 cut(s) 96, 225, 929, 977, 1345, 1372, 1412
DdeI CTNAG 4 cut(s) 127, 518, 578, 1465
DinI GGCGCC 1 cut(s) 380
DpnI GATC 7 cut(s) 207, 405, 662, 885, 1107, 1600, 1806
DpnII GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
DriI GACNNNNNGTC 1 cut(s) 903
EaeI YGGCCR 3 cut(s) 1048, 1215, 1706
EagI CGGCCG 2 cut(s) 1048, 1706
Eam1104I CTCTTC 1 cut(s) 171
Eam1105I GACNNNNNGTC 1 cut(s) 903
EarI CTCTTC 1 cut(s) 171
EclXI CGGCCG 2 cut(s) 1048, 1706
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 3 cut(s) 297, 1233, 1881
Eco47I GGWCC 3 cut(s) 314, 1319, 1868
Eco52I CGGCCG 2 cut(s) 1048, 1706
Eco57I CTGAAG 3 cut(s) 222, 452, 1796
EcoNI CCTNNNNNAGG 2 cut(s) 1307, 1752
EcoRII CCWGG 1 cut(s) 1880
EcoT14I CCWWGG 1 cut(s) 1218
EcoT38I GRGCYC 3 cut(s) 297, 1233, 1881
EgeI GGCGCC 1 cut(s) 380
EheI GGCGCC 1 cut(s) 380
ErhI CCWWGG 1 cut(s) 1218
FaeI CATG 9 cut(s) 268, 404, 740, 1135, 1464, 1639, 1664, 1683, 1754
FalI AAGNNNNNCTT 6 cut(s) 76, 108, 715, 747, 1325, 1357
FaqI GGGAC 1 cut(s) 596
FatI CATG 9 cut(s) 264, 400, 736, 1131, 1460, 1635, 1660, 1679, 1750
FauNDI CATATG 1 cut(s) 1581
FbaI TGATCA 2 cut(s) 660, 1105
FokI GGATG 4 cut(s) 425, 646, 1235, 1337
FriOI GRGCYC 3 cut(s) 297, 1233, 1881
FspAI RTGCGCAY 1 cut(s) 1684
FspBI CTAG 7 cut(s) 258, 489, 773, 1034, 1265, 1478, 1928
FspI TGCGCA 2 cut(s) 698, 1684
GlaI GCGC 6 cut(s) 380, 650, 698, 939, 1684, 1720
GsuI CTGGAG 2 cut(s) 203, 1743
HaeII RGCGCY 2 cut(s) 382, 941
HaeIII GGCC 6 cut(s) 443, 995, 1030, 1050, 1217, 1708
HapII CCGG 6 cut(s) 652, 908, 996, 1322, 1494, 1705
HgaI GACGC 2 cut(s) 257, 1042
HhaI GCGC 6 cut(s) 381, 651, 699, 940, 1685, 1721
Hin1I GRCGYC 1 cut(s) 379
Hin1II CATG 9 cut(s) 268, 404, 740, 1135, 1464, 1639, 1664, 1683, 1754
Hin6I GCGC 6 cut(s) 379, 649, 697, 938, 1683, 1719
HinP1I GCGC 6 cut(s) 379, 649, 697, 938, 1683, 1719
HincII GTYRAC 1 cut(s) 1297
HindII GTYRAC 1 cut(s) 1297
HindIII AAGCTT 2 cut(s) 356, 515
HinfI GANTC 7 cut(s) 584, 952, 967, 999, 1248, 1562, 1796
HpaII CCGG 6 cut(s) 652, 908, 996, 1322, 1494, 1705
HphI GGTGA 2 cut(s) 286, 1694
Hpy166II GTNNAC 5 cut(s) 277, 1297, 1414, 1766, 1868
Hpy188I TCNGA 8 cut(s) 505, 598, 819, 1010, 1384, 1491, 1557, 1815
Hpy188III TCNNGA 7 cut(s) 80, 200, 737, 881, 908, 1322, 1733
Hpy8I GTNNAC 5 cut(s) 277, 1297, 1414, 1766, 1868
Hpy99I CGWCG 2 cut(s) 1058, 1659
HpyAV CCTTC 8 cut(s) 63, 277, 433, 454, 788, 1066, 1272, 1791
HpyCH4III ACNGT 1 cut(s) 1504
HpyF10VI GCNNNNNNNGC 7 cut(s) 461, 799, 867, 940, 1466, 1520, 1626
HpyF3I CTNAG 4 cut(s) 127, 518, 578, 1465
Hsp92I GRCGYC 1 cut(s) 379
Hsp92II CATG 9 cut(s) 268, 404, 740, 1135, 1464, 1639, 1664, 1683, 1754
HspAI GCGC 6 cut(s) 379, 649, 697, 938, 1683, 1719
KasI GGCGCC 1 cut(s) 378
Kpn2I TCCGGA 2 cut(s) 907, 1321
Ksp22I TGATCA 2 cut(s) 660, 1105
KspI CCGCGG 1 cut(s) 944
Kzo9I GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 2 cut(s) 492, 1236
LweI GCATC 6 cut(s) 106, 442, 534, 624, 1452, 1585
MaeI CTAG 7 cut(s) 258, 489, 773, 1034, 1265, 1478, 1928
MaeIII GTNAC 2 cut(s) 1151, 1174
MalI GATC 7 cut(s) 207, 405, 662, 885, 1107, 1600, 1806
MboI GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
MboII GAAGA 8 cut(s) 61, 188, 215, 445, 448, 469, 1682, 1748
MfeI CAATTG 1 cut(s) 747
MflI RGATCY 1 cut(s) 1804
MhlI GDGCHC 5 cut(s) 297, 609, 1080, 1233, 1881
MluCI AATT 5 cut(s) 21, 473, 701, 747, 761
Mly113I GGCGCC 1 cut(s) 379
MlyI GAGTC 4 cut(s) 578, 1008, 1257, 1571
MmeI TCCRAC 3 cut(s) 390, 665, 737
MroI TCCGGA 2 cut(s) 907, 1321
MseI TTAA 4 cut(s) 38, 195, 360, 987
MslI CAYNNNNRTG 3 cut(s) 1101, 1170, 1678
MspA1I CMGCKG 2 cut(s) 712, 943
MspCI CTTAAG 2 cut(s) 359, 986
MspI CCGG 6 cut(s) 652, 908, 996, 1322, 1494, 1705
MspR9I CCNGG 2 cut(s) 653, 1882
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 1139
MvaI CCWGG 1 cut(s) 1882
MvnI CGCG 4 cut(s) 943, 1053, 1719, 1892
MwoI GCNNNNNNNGC 7 cut(s) 461, 799, 867, 940, 1466, 1520, 1626
NarI GGCGCC 1 cut(s) 379
NciI CCSGG 1 cut(s) 653
NdeI CATATG 1 cut(s) 1581
NdeII GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
NlaIII CATG 9 cut(s) 268, 404, 740, 1135, 1464, 1639, 1664, 1683, 1754
NlaIV GGNNCC 3 cut(s) 255, 380, 1232
NmeAIII GCCGAG 2 cut(s) 1193, 1630
NmuCI GTSAC 1 cut(s) 1151
NsbI TGCGCA 2 cut(s) 698, 1684
NspI RCATGY 1 cut(s) 1464
NspV TTCGAA 2 cut(s) 1126, 1143
PaeI GCATGC 1 cut(s) 1464
PagI TCATGA 1 cut(s) 736
PciSI GCTCTTC 1 cut(s) 171
PctI GAATGC 1 cut(s) 1139
PfeI GAWTC 3 cut(s) 952, 967, 1796
PleI GAGTC 4 cut(s) 578, 1007, 1256, 1570
PluTI GGCGCC 1 cut(s) 382
PpsI GAGTC 4 cut(s) 578, 1007, 1256, 1570
PsiI TTATAA 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 1880
PspGI CCWGG 1 cut(s) 1880
PspN4I GGNNCC 3 cut(s) 255, 380, 1232
PspPI GGNCC 4 cut(s) 314, 1028, 1319, 1868
PstNI CAGNNNCTG 1 cut(s) 1807
PsuI RGATCY 1 cut(s) 1804
PvuII CAGCTG 1 cut(s) 712
RsaI GTAC 7 cut(s) 97, 226, 930, 978, 1346, 1373, 1413
RsaNI GTAC 7 cut(s) 96, 225, 929, 977, 1345, 1372, 1412
RseI CAYNNNNRTG 3 cut(s) 1101, 1170, 1678
SacII CCGCGG 1 cut(s) 944
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 4 cut(s) 38, 195, 360, 987
Sau3AI GATC 7 cut(s) 205, 403, 660, 883, 1105, 1598, 1804
Sau96I GGNCC 4 cut(s) 314, 1028, 1319, 1868
ScaI AGTACT 2 cut(s) 226, 1373
SchI GAGTC 4 cut(s) 578, 1008, 1257, 1571
ScrFI CCNGG 2 cut(s) 653, 1882
SduI GDGCHC 5 cut(s) 297, 609, 1080, 1233, 1881
SfaNI GCATC 6 cut(s) 106, 442, 534, 624, 1452, 1585
SfcI CTRYAG 2 cut(s) 1375, 1936
SfoI GGCGCC 1 cut(s) 380
Sfr303I CCGCGG 1 cut(s) 944
SfuI TTCGAA 2 cut(s) 1126, 1143
SgrBI CCGCGG 1 cut(s) 944
SinI GGWCC 3 cut(s) 314, 1319, 1868
SmiMI CAYNNNNRTG 3 cut(s) 1101, 1170, 1678
SmlI CTYRAG 3 cut(s) 359, 986, 1023
SmoI CTYRAG 3 cut(s) 359, 986, 1023
SphI GCATGC 1 cut(s) 1464
Sse9I AATT 5 cut(s) 21, 473, 701, 747, 761
SsiI CCGC 8 cut(s) 485, 794, 830, 941, 943, 1018, 1051, 1898
SspDI GGCGCC 1 cut(s) 378
SspMI CTAG 7 cut(s) 258, 489, 773, 1034, 1265, 1478, 1928
StyD4I CCNGG 2 cut(s) 651, 1880
StyI CCWWGG 1 cut(s) 1218
TaaI ACNGT 1 cut(s) 1504
TaqI TCGA 6 cut(s) 669, 1090, 1126, 1143, 1197, 1794
TaqII GACCGA 2 cut(s) 302, 731
TasI AATT 5 cut(s) 21, 473, 701, 747, 761
TatI WGTACW 5 cut(s) 95, 224, 976, 1344, 1371
TauI GCSGC 5 cut(s) 488, 796, 943, 946, 1053
TfiI GAWTC 3 cut(s) 952, 967, 1796
Tru1I TTAA 4 cut(s) 38, 195, 360, 987
Tru9I TTAA 4 cut(s) 38, 195, 360, 987
TscAI CASTG 1 cut(s) 1516
TseFI GTSAC 1 cut(s) 1151
Tsp45I GTSAC 1 cut(s) 1151
TspDTI ATGAA 4 cut(s) 432, 553, 1598, 1677
TspGWI ACGGA 2 cut(s) 342, 1260
TspRI CASTG 1 cut(s) 1516
Vha464I CTTAAG 2 cut(s) 359, 986
VpaK11BI GGWCC 3 cut(s) 314, 1319, 1868
XagI CCTNNNNNAGG 2 cut(s) 1307, 1752
XceI RCATGY 1 cut(s) 1464
XcmI CCANNNNNNNNNTGG 2 cut(s) 149, 753
XspI CTAG 7 cut(s) 258, 489, 773, 1034, 1265, 1478, 1928
ZrmI AGTACT 2 cut(s) 226, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.