FvH4_4g30360
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0006868
Physical Location & Seq
Reverse (-)
30030743 .. 30034393
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g30360.t4

Sequence Viewer

Length: 942 bp
ATGGACGGCGACAAGGACACACGCGATCCGGGACTCACCGACTTCCCGAGCTGGGACAACGCCTACTTCAGTGAATTCGGCTGGAATTTCAACCCGGATGACCCGATCGGCACCGACGACAGGTCTGATTTGGCGGGAACCAGCAGTACTACAACTCTGCAGGCTGAACAAGTTCCCGAAAGAAGCAGCAATACTAGCTCCCGATCAGACCCTGCCCCCGTGGTCGGATCTGTCTCCTCAAGCTCAACTGAGGATCCGCCAGAGAGGTCTACTGGCTCCGGCGGCACGCCACCACTCGAAACACCGAGTAGCACTACGAAGGTGAACAAAAAGAAGGGGCAGAAGCGAATCCGGCAGCCAAGATTTGCGTTTATGACTAAGAGTGAAGTTGATCATCTTGAGGATGGCTACCGTTGGCGGAAATATGGACAGAAGGCTGTCAAGAATAGCCCATATCCGAGGAGCTACTATCGGTGCACAAATAGCAAATGCACTGTGAAGAAAAGGGTTGAGCGTTCCTCAGAAGACCCGACGACAGTAATAACGACGTATGAAGGGCAACACTGCCATCATACGGTTTCATTTCCCCGCGGCAGCGGCAGCGGGGGAGTCAATATTCTTAGTCATGACCAATCTGGCTTTGCCGCAGGAAGCCATCAGTTAATGCAGCTTCCTCCAGTCTCACAGTTCATGCATTTTCCAACTTCAATTCAACCCAGATCACAAATTAGTCCAACTCATAATTTGACAGTAGGGAGCTCGCAGTCGCATCGATTTGTCCCGGCTGGTGGTACTCATAACCATTATGATGAAGCAGCTGGAGGATCCCAGCGCATTACTAATCAAAACCATGAGCCACTCCCAGTAGTACCAGTTCCTACAGATGAAGGGCTTCTTGGGGACATTGTACCTCCTGGAATGCGTAATCCGTTTGATGGATAA

Protein Analysis

314

Amino Acids

34.19

Weight (kDa)

6.45

Isoelectric Point (pI)

65.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 134 - 190 9.8e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AccI GTMKAC 1 cut(s) 269
AccII CGCG 2 cut(s) 24, 591
AciI CCGC 9 cut(s) 134, 257, 282, 418, 589, 591, 597, 603, 645
AclWI GGATC 6 cut(s) 20, 235, 248, 261, 819, 832
AcsI RAATTY 2 cut(s) 74, 85
AcuI CTGAAG 1 cut(s) 52
AfaI GTAC 4 cut(s) 148, 793, 870, 909
AfiI CCNNNNNNNGG 6 cut(s) 52, 120, 224, 574, 788, 935
AgsI TTSAA 3 cut(s) 91, 708, 713
AhdI GACNNNNNGTC 1 cut(s) 121
AjnI CCWGG 1 cut(s) 913
AjuI GAANNNNNNNTTGG 2 cut(s) 879, 911
AluBI AGCT 7 cut(s) 51, 198, 243, 465, 670, 759, 818
AluI AGCT 7 cut(s) 51, 198, 243, 465, 670, 759, 818
Alw21I GWGCWC 2 cut(s) 479, 761
Alw26I GTCTC 2 cut(s) 238, 685
Alw44I GTGCAC 1 cut(s) 475
AlwI GGATC 6 cut(s) 20, 235, 248, 261, 819, 832
AlwNI CAGNNNCTG 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 46
ApaLI GTGCAC 1 cut(s) 475
ApeKI GCWGC 6 cut(s) 186, 355, 594, 600, 667, 815
ApoI RAATTY 2 cut(s) 74, 85
Asp700I GAANNNNTTC 2 cut(s) 171, 891
AspLEI GCGC 1 cut(s) 834
AsuC2I CCSGG 3 cut(s) 30, 95, 782
AsuHPI GGTGA 2 cut(s) 28, 334
AvaI CYCGRG 1 cut(s) 46
BaeGI GKGCMC 1 cut(s) 479
BamHI GGATCC 2 cut(s) 253, 824
BanI GGYRCC 1 cut(s) 110
BanII GRGCYC 1 cut(s) 761
BarI GAAGNNNNNNTAC 2 cut(s) 175, 207
BbsI GAAGAC 1 cut(s) 531
Bbv12I GWGCWC 2 cut(s) 479, 761
BbvI GCAGC 6 cut(s) 198, 367, 606, 612, 679, 827
BccI CCATC 4 cut(s) 398, 576, 663, 929
BceAI ACGGC 1 cut(s) 22
BcgI CGANNNNNNTGC 2 cut(s) 752, 786
BciT130I CCWGG 1 cut(s) 915
BclI TGATCA 1 cut(s) 391
BcnI CCSGG 3 cut(s) 30, 95, 782
BcoDI GTCTC 2 cut(s) 238, 685
BfaI CTAG 1 cut(s) 195
BfmI CTRYAG 2 cut(s) 158, 879
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 4 cut(s) 30, 95, 782, 915
BmeRI GACNNNNNGTC 1 cut(s) 121
BmeT110I CYCGRG 1 cut(s) 46
BmiI GGNNCC 5 cut(s) 112, 139, 255, 277, 826
BmrFI CCNGG 4 cut(s) 30, 95, 782, 915
BmrI ACTGGG 1 cut(s) 857
BmsI GCATC 1 cut(s) 778
BmuI ACTGGG 1 cut(s) 857
BpiI GAAGAC 1 cut(s) 531
BplI GAGNNNNNCTC 2 cut(s) 503, 535
BpmI CTGGAG 2 cut(s) 660, 840
BpuEI CTTGAG 2 cut(s) 223, 419
BpuMI CCSGG 3 cut(s) 30, 95, 782
Bsa29I ATCGAT 1 cut(s) 772
BsaJI CCNNGG 3 cut(s) 219, 458, 589
Bsc4I CCNNNNNNNGG 6 cut(s) 52, 120, 224, 574, 788, 935
Bse1I ACTGG 4 cut(s) 277, 677, 863, 872
BseBI CCWGG 1 cut(s) 915
BseCI ATCGAT 1 cut(s) 772
BseDI CCNNGG 3 cut(s) 219, 458, 589
BseGI GGATG 2 cut(s) 103, 409
BseLI CCNNNNNNNGG 6 cut(s) 52, 120, 224, 574, 788, 935
BseMII CTCAG 2 cut(s) 240, 534
BseNI ACTGG 4 cut(s) 277, 677, 863, 872
BseRI GAGGAG 2 cut(s) 226, 475
BseSI GKGCMC 1 cut(s) 479
BseXI GCAGC 6 cut(s) 198, 367, 606, 612, 679, 827
BseYI CCCAGC 2 cut(s) 51, 828
Bsh1236I CGCG 2 cut(s) 24, 591
Bsh1285I CGRYCG 1 cut(s) 108
BshNI GGYRCC 1 cut(s) 110
BshVI ATCGAT 1 cut(s) 772
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 479, 761
BsiHKCI CYCGRG 1 cut(s) 46
BsiSI CCGG 5 cut(s) 29, 95, 279, 352, 782
BslFI GGGAC 4 cut(s) 45, 68, 764, 914
BslI CCNNNNNNNGG 6 cut(s) 52, 120, 224, 574, 788, 935
BsmAI GTCTC 2 cut(s) 238, 685
BsmFI GGGAC 4 cut(s) 45, 68, 764, 914
BsmI GAATGC 1 cut(s) 924
BsoBI CYCGRG 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 479, 761
Bsp143I GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
BspACI CCGC 9 cut(s) 134, 257, 282, 418, 589, 591, 597, 603, 645
BspCNI CTCAG 2 cut(s) 241, 533
BspDI ATCGAT 1 cut(s) 772
BspFNI CGCG 2 cut(s) 24, 591
BspHI TCATGA 1 cut(s) 625
BspLI GGNNCC 5 cut(s) 112, 139, 255, 277, 826
BspMAI CTGCAG 1 cut(s) 162
BspPI GGATC 6 cut(s) 20, 235, 248, 261, 819, 832
BspT107I GGYRCC 1 cut(s) 110
BsrI ACTGG 4 cut(s) 277, 677, 863, 872
BssECI CCNNGG 3 cut(s) 219, 458, 589
BssMI GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
Bst2UI CCWGG 1 cut(s) 915
Bst4CI ACNGT 6 cut(s) 413, 496, 538, 577, 687, 751
BstC8I GCNNGC 3 cut(s) 162, 287, 761
BstDEI CTNAG 4 cut(s) 249, 378, 520, 620
BstDSI CCRYGG 2 cut(s) 219, 589
BstF5I GGATG 2 cut(s) 103, 409
BstFNI CGCG 2 cut(s) 24, 591
BstHHI GCGC 1 cut(s) 834
BstKTI GATC 8 cut(s) 28, 108, 206, 230, 256, 394, 722, 827
BstMAI GTCTC 2 cut(s) 238, 685
BstMBI GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
BstMCI CGRYCG 1 cut(s) 108
BstMWI GCNNNNNNNGC 6 cut(s) 195, 282, 352, 483, 597, 600
BstNI CCWGG 1 cut(s) 915
BstSCI CCNGG 4 cut(s) 28, 93, 780, 913
BstSFI CTRYAG 2 cut(s) 158, 879
BstSLI GKGCMC 1 cut(s) 479
BstUI CGCG 2 cut(s) 24, 591
BstV1I GCAGC 6 cut(s) 198, 367, 606, 612, 679, 827
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 3 cut(s) 227, 253, 824
BstYI RGATCY 3 cut(s) 227, 253, 824
Bsu15I ATCGAT 1 cut(s) 772
BsuTUI ATCGAT 1 cut(s) 772
BtgI CCRYGG 2 cut(s) 219, 589
BtsCI GGATG 2 cut(s) 103, 409
BtsI GCAGTG 1 cut(s) 562
BtsIMutI CAGTG 3 cut(s) 76, 492, 562
Cac8I GCNNGC 3 cut(s) 162, 287, 761
CaiI CAGNNNCTG 1 cut(s) 212
CciI TCATGA 1 cut(s) 625
CfoI GCGC 1 cut(s) 834
Cfr42I CCGCGG 1 cut(s) 592
ClaI ATCGAT 1 cut(s) 772
Csp6I GTAC 4 cut(s) 147, 792, 869, 908
CviAII CATG 3 cut(s) 626, 691, 851
CviQI GTAC 4 cut(s) 147, 792, 869, 908
DdeI CTNAG 4 cut(s) 249, 378, 520, 620
DpnI GATC 8 cut(s) 27, 107, 205, 229, 255, 393, 721, 826
DpnII GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
DriI GACNNNNNGTC 1 cut(s) 121
Eam1105I GACNNNNNGTC 1 cut(s) 121
EciI GGCGGA 2 cut(s) 246, 433
Ecl136II GAGCTC 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 761
Eco53kI GAGCTC 1 cut(s) 759
Eco57I CTGAAG 1 cut(s) 52
Eco88I CYCGRG 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 759
EcoRI GAATTC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 913
EcoT22I ATGCAT 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 761
FaeI CATG 3 cut(s) 629, 694, 854
FalI AAGNNNNNCTT 2 cut(s) 879, 911
FaqI GGGAC 4 cut(s) 45, 68, 764, 914
FatI CATG 3 cut(s) 625, 690, 850
FauI CCCGC 3 cut(s) 127, 596, 596
FbaI TGATCA 1 cut(s) 391
FblI GTMKAC 1 cut(s) 269
FokI GGATG 2 cut(s) 110, 416
FriOI GRGCYC 1 cut(s) 761
FspBI CTAG 1 cut(s) 195
GlaI GCGC 1 cut(s) 833
GsaI CCCAGC 2 cut(s) 55, 832
GsuI CTGGAG 2 cut(s) 660, 840
HapII CCGG 5 cut(s) 29, 95, 279, 352, 782
HhaI GCGC 1 cut(s) 834
Hin1II CATG 3 cut(s) 629, 694, 854
Hin6I GCGC 1 cut(s) 832
HinP1I GCGC 1 cut(s) 832
HinfI GANTC 3 cut(s) 33, 348, 609
HpaII CCGG 5 cut(s) 29, 95, 279, 352, 782
HphI GGTGA 2 cut(s) 28, 334
Hpy166II GTNNAC 3 cut(s) 270, 325, 477
Hpy188I TCNGA 5 cut(s) 127, 208, 227, 459, 523
Hpy188III TCNNGA 6 cut(s) 46, 176, 201, 398, 442, 626
Hpy8I GTNNAC 3 cut(s) 270, 325, 477
Hpy99I CGWCG 3 cut(s) 119, 535, 550
HpyAV CCTTC 5 cut(s) 313, 328, 427, 548, 881
HpyCH4III ACNGT 6 cut(s) 413, 496, 538, 577, 687, 751
HpyCH4IV ACGT 1 cut(s) 548
HpyCH4V TGCA 5 cut(s) 160, 477, 492, 667, 694
HpyF10VI GCNNNNNNNGC 6 cut(s) 195, 282, 352, 483, 597, 600
HpyF3I CTNAG 4 cut(s) 249, 378, 520, 620
HpySE526I ACGT 1 cut(s) 548
Hsp92II CATG 3 cut(s) 629, 694, 854
HspAI GCGC 1 cut(s) 832
Ksp22I TGATCA 1 cut(s) 391
KspI CCGCGG 1 cut(s) 592
Kzo9I GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
LmnI GCTCC 4 cut(s) 203, 281, 462, 756
Lsp1109I GCAGC 6 cut(s) 198, 367, 606, 612, 679, 827
LweI GCATC 1 cut(s) 778
MaeI CTAG 1 cut(s) 195
MaeII ACGT 1 cut(s) 548
MalI GATC 8 cut(s) 27, 107, 205, 229, 255, 393, 721, 826
MboI GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
MboII GAAGA 2 cut(s) 511, 536
MflI RGATCY 3 cut(s) 227, 253, 824
MhlI GDGCHC 2 cut(s) 479, 761
MluCI AATT 5 cut(s) 74, 85, 708, 726, 742
MlyI GAGTC 2 cut(s) 27, 618
MmeI TCCRAC 3 cut(s) 205, 725, 758
MnlI CCTC 9 cut(s) 244, 247, 258, 394, 453, 529, 684, 815, 921
Mph1103I ATGCAT 1 cut(s) 696
MroXI GAANNNNTTC 2 cut(s) 171, 891
MseI TTAA 1 cut(s) 662
MslI CAYNNNNRTG 1 cut(s) 807
MspA1I CMGCKG 4 cut(s) 591, 597, 603, 818
MspI CCGG 5 cut(s) 29, 95, 279, 352, 782
MspR9I CCNGG 4 cut(s) 30, 95, 782, 915
Mva1269I GAATGC 1 cut(s) 924
MvaI CCWGG 1 cut(s) 915
MvnI CGCG 2 cut(s) 24, 591
MwoI GCNNNNNNNGC 6 cut(s) 195, 282, 352, 483, 597, 600
NciI CCSGG 3 cut(s) 30, 95, 782
NdeII GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
NlaIII CATG 3 cut(s) 629, 694, 854
NlaIV GGNNCC 5 cut(s) 112, 139, 255, 277, 826
NsiI ATGCAT 1 cut(s) 696
PagI TCATGA 1 cut(s) 625
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PctI GAATGC 1 cut(s) 924
PdmI GAANNNNTTC 2 cut(s) 171, 891
PfeI GAWTC 1 cut(s) 348
PfoI TCCNGGA 2 cut(s) 28, 913
Ple19I CGATCG 1 cut(s) 108
PleI GAGTC 2 cut(s) 27, 617
PpsI GAGTC 2 cut(s) 27, 617
Psp124BI GAGCTC 1 cut(s) 761
Psp6I CCWGG 1 cut(s) 913
PspFI CCCAGC 2 cut(s) 51, 828
PspGI CCWGG 1 cut(s) 913
PspN4I GGNNCC 5 cut(s) 112, 139, 255, 277, 826
PsrI GAACNNNNNNTAC 4 cut(s) 130, 162, 858, 890
PstI CTGCAG 1 cut(s) 162
PstNI CAGNNNCTG 1 cut(s) 212
PsuI RGATCY 3 cut(s) 227, 253, 824
PvuI CGATCG 1 cut(s) 108
PvuII CAGCTG 1 cut(s) 818
RsaI GTAC 4 cut(s) 148, 793, 870, 909
RsaNI GTAC 4 cut(s) 147, 792, 869, 908
RseI CAYNNNNRTG 1 cut(s) 807
SacI GAGCTC 1 cut(s) 761
SacII CCGCGG 1 cut(s) 592
SaqAI TTAA 1 cut(s) 662
Sau3AI GATC 8 cut(s) 25, 105, 203, 227, 253, 391, 719, 824
ScaI AGTACT 1 cut(s) 148
SchI GAGTC 2 cut(s) 27, 618
ScrFI CCNGG 4 cut(s) 30, 95, 782, 915
SduI GDGCHC 2 cut(s) 479, 761
SfaNI GCATC 1 cut(s) 778
SfcI CTRYAG 2 cut(s) 158, 879
Sfr303I CCGCGG 1 cut(s) 592
SgrBI CCGCGG 1 cut(s) 592
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 2 cut(s) 238, 398
SmoI CTYRAG 2 cut(s) 238, 398
Sse9I AATT 5 cut(s) 74, 85, 708, 726, 742
SsiI CCGC 9 cut(s) 134, 257, 282, 418, 589, 591, 597, 603, 645
SspI AATATT 1 cut(s) 616
SspMI CTAG 1 cut(s) 195
SstI GAGCTC 1 cut(s) 761
StyD4I CCNGG 4 cut(s) 28, 93, 780, 913
TaaI ACNGT 6 cut(s) 413, 496, 538, 577, 687, 751
TaiI ACGT 1 cut(s) 551
TaqI TCGA 2 cut(s) 297, 772
TasI AATT 5 cut(s) 74, 85, 708, 726, 742
TatI WGTACW 1 cut(s) 146
TauI GCSGC 4 cut(s) 285, 594, 600, 647
TfiI GAWTC 1 cut(s) 348
Tru1I TTAA 1 cut(s) 662
Tru9I TTAA 1 cut(s) 662
TscAI CASTG 3 cut(s) 76, 499, 569
TseI GCWGC 6 cut(s) 186, 355, 594, 600, 667, 815
TspDTI ATGAA 5 cut(s) 567, 570, 679, 825, 900
TspGWI ACGGA 1 cut(s) 918
TspRI CASTG 3 cut(s) 76, 499, 569
VneI GTGCAC 1 cut(s) 475
XapI RAATTY 2 cut(s) 74, 85
XmiI GTMKAC 1 cut(s) 269
XmnI GAANNNNTTC 2 cut(s) 171, 891
XspI CTAG 1 cut(s) 195
ZrmI AGTACT 1 cut(s) 148
Zsp2I ATGCAT 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.