Rw0G007130
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00270
OG0006868
Physical Location & Seq
Reverse (-)
15569 .. 17871
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G007130.1

Sequence Viewer

Length: 963 bp
ATGGACGGCGACAAGGACTCACGCGATCCGGGACTCACCGAGTTCCCCACCGAGCCGAGCTGGGACAGTCCTTACTTCTTCAGCAACGATCGTGACACCACCGTACTAAGCGAATTCGGGTGGAATTTCAGCCCCGACGACCAGATCGGCTCCGACGACAGGTCCGATTTGGCGGGAAGCAGCACTACTACTACTCTCGCGGAACAGTTTCCAGAAAGAAGCAGTAATACTAACTCCGACCCGGCCCCCGTGGTCGGATCTGTTTCGTCAAGCTCTACAGAGGATCCGCCGGAGAGGTCTACTGGCTCCGGCTCCGGCGGCACACCACCTCCCGAGACACCCACTACGAAGGTGAATAAAAAGAAGGGGCAGAAGCGAATCCGGCAGCCAAGATTTGCGTTCATGACAAAGAGTGAAGTTGATCATCTCGAGGATGGCTACCGTTGGCGGAAATATGGACAGAAGGCTGTTAAGAATAGCCCATATCCAAGGAGCTACTATCGGTGCACAAATAGCAAATGCACTGTGAAGAAAAGGGTTGAACGTTCCTCTGAAGATCCAACGACGGTAATTACGACGTATGAAGGGCAACACTGTCATCATACCGTTGCATTTCCCCGCGGCGGCGGGGGAGTCAATATTATTAGTCATGATCAATCTGGCTTTGCCGCAGGAAGCCAGTTAATGCAGCTTTCTCCAGTCTCGCAGTTCATGCATTATCCAACTTCAATTCAACCCAGATCACAAATTAGTCCAACTCATAATTTGACAGTAGGAGGGTCGCAGTCGCACCGATTAGTCCGGGCTGGCGGTACTCATTACCATCATGATCAAGCAGCTGGGGGATCCCAGTGCATTATTAATCCAAATCATGAGCCACTCCCAGTAGTACCAGTTCCTACAGATGAAGGGCTTCTTGGGGACATTGTACCTCCTGGAATGCGTAATCCGTTCGATGGATAA

Protein Analysis

320

Amino Acids

34.94

Weight (kDa)

6.17

Isoelectric Point (pI)

67.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 144 - 200 1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 299
AccII CGCG 3 cut(s) 24, 200, 621
AclI AACGTT 1 cut(s) 544
AclWI GGATC 7 cut(s) 20, 265, 278, 291, 551, 840, 853
AcsI RAATTY 2 cut(s) 113, 124
AcuI CTGAAG 2 cut(s) 64, 573
AfaI GTAC 4 cut(s) 105, 814, 891, 930
AfiI CCNNNNNNNGG 4 cut(s) 159, 254, 623, 956
AgsI TTSAA 3 cut(s) 542, 729, 734
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 1 cut(s) 934
AjuI GAANNNNNNNTTGG 2 cut(s) 900, 932
AluBI AGCT 5 cut(s) 60, 273, 495, 691, 839
AluI AGCT 5 cut(s) 60, 273, 495, 691, 839
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 2 cut(s) 329, 706
Alw44I GTGCAC 1 cut(s) 505
AlwI GGATC 7 cut(s) 20, 265, 278, 291, 551, 840, 853
Ama87I CYCGRG 2 cut(s) 332, 428
AoxI GGCC 1 cut(s) 243
ApaLI GTGCAC 1 cut(s) 505
ApeKI GCWGC 4 cut(s) 180, 385, 688, 836
ApoI RAATTY 2 cut(s) 113, 124
AseI ATTAAT 1 cut(s) 861
Asp700I GAANNNNTTC 2 cut(s) 207, 912
AspS9I GGNCC 2 cut(s) 162, 244
AsuC2I CCSGG 3 cut(s) 30, 242, 803
AsuHPI GGTGA 2 cut(s) 28, 364
AvaI CYCGRG 2 cut(s) 332, 428
AvaII GGWCC 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 509
BamHI GGATCC 2 cut(s) 283, 845
BarI GAAGNNNNNNTAC 4 cut(s) 169, 201, 211, 243
Bbv12I GWGCWC 1 cut(s) 509
BbvI GCAGC 4 cut(s) 192, 397, 700, 848
BccI CCATC 3 cut(s) 428, 831, 950
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 936
BclI TGATCA 3 cut(s) 421, 652, 829
BcnI CCSGG 3 cut(s) 30, 242, 803
BcoDI GTCTC 2 cut(s) 329, 706
BfmI CTRYAG 2 cut(s) 276, 900
BisI GCNGC 8 cut(s) 181, 319, 386, 622, 625, 669, 689, 837
BlsI GCNGC 8 cut(s) 182, 320, 387, 623, 626, 670, 690, 838
Bme1390I CCNGG 4 cut(s) 30, 242, 803, 936
Bme18I GGWCC 1 cut(s) 162
BmeRI GACNNNNNGTC 1 cut(s) 160
BmeT110I CYCGRG 2 cut(s) 332, 428
BmgT120I GGNCC 2 cut(s) 162, 244
BmiI GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 847
BmrFI CCNGG 4 cut(s) 30, 242, 803, 936
BmrI ACTGGG 2 cut(s) 844, 878
BmuI ACTGGG 2 cut(s) 844, 878
BpmI CTGGAG 1 cut(s) 681
BpuMI CCSGG 3 cut(s) 30, 242, 803
BsaJI CCNNGG 3 cut(s) 249, 488, 619
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 254, 623, 956
Bse1I ACTGG 6 cut(s) 307, 679, 698, 850, 884, 893
BseBI CCWGG 1 cut(s) 936
BseDI CCNNGG 3 cut(s) 249, 488, 619
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 4 cut(s) 159, 254, 623, 956
BseNI ACTGG 6 cut(s) 307, 679, 698, 850, 884, 893
BseSI GKGCMC 1 cut(s) 509
BseXI GCAGC 4 cut(s) 192, 397, 700, 848
BseYI CCCAGC 2 cut(s) 60, 839
Bsh1236I CGCG 3 cut(s) 24, 200, 621
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 245
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 509
BsiHKCI CYCGRG 2 cut(s) 332, 428
BsiSI CCGG 7 cut(s) 29, 242, 290, 309, 315, 382, 802
BslFI GGGAC 3 cut(s) 45, 77, 935
BslI CCNNNNNNNGG 4 cut(s) 159, 254, 623, 956
BsmAI GTCTC 2 cut(s) 329, 706
BsmFI GGGAC 3 cut(s) 45, 77, 935
BsmI GAATGC 1 cut(s) 945
BsnI GGCC 1 cut(s) 245
BsoBI CYCGRG 2 cut(s) 332, 428
Bsp1286I GDGCHC 1 cut(s) 509
BspANI GGCC 1 cut(s) 245
BspFNI CGCG 3 cut(s) 24, 200, 621
BspHI TCATGA 4 cut(s) 402, 649, 826, 871
BspLI GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 847
BspPI GGATC 7 cut(s) 20, 265, 278, 291, 551, 840, 853
BsrI ACTGG 6 cut(s) 307, 679, 698, 850, 884, 893
BssECI CCNNGG 3 cut(s) 249, 488, 619
BssT1I CCWWGG 1 cut(s) 488
Bst2UI CCWGG 1 cut(s) 936
Bst4CI ACNGT 9 cut(s) 68, 103, 207, 443, 526, 568, 596, 607, 772
BstAPI GCANNNNNTGC 1 cut(s) 712
BstC8I GCNNGC 1 cut(s) 808
BstDEI CTNAG 1 cut(s) 107
BstDSI CCRYGG 2 cut(s) 249, 619
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 3 cut(s) 24, 200, 621
BstMAI GTCTC 2 cut(s) 329, 706
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 4 cut(s) 318, 382, 513, 712
BstNI CCWGG 1 cut(s) 936
BstSCI CCNGG 4 cut(s) 28, 240, 801, 934
BstSFI CTRYAG 2 cut(s) 276, 900
BstSLI GKGCMC 1 cut(s) 509
BstUI CGCG 3 cut(s) 24, 200, 621
BstV1I GCAGC 4 cut(s) 192, 397, 700, 848
BstX2I RGATCY 4 cut(s) 257, 283, 556, 845
BstYI RGATCY 4 cut(s) 257, 283, 556, 845
BsuRI GGCC 1 cut(s) 245
BtgI CCRYGG 2 cut(s) 249, 619
BtsCI GGATG 1 cut(s) 439
BtsIMutI CAGTG 3 cut(s) 522, 592, 857
Cac8I GCNNGC 1 cut(s) 808
CciI TCATGA 4 cut(s) 402, 649, 826, 871
Cfr13I GGNCC 2 cut(s) 162, 244
Cfr42I CCGCGG 1 cut(s) 622
Csp6I GTAC 4 cut(s) 104, 813, 890, 929
CviAII CATG 5 cut(s) 403, 650, 712, 827, 872
CviQI GTAC 4 cut(s) 104, 813, 890, 929
DdeI CTNAG 1 cut(s) 107
DriI GACNNNNNGTC 1 cut(s) 160
Eam1105I GACNNNNNGTC 1 cut(s) 160
EciI GGCGGA 2 cut(s) 276, 463
Eco130I CCWWGG 1 cut(s) 488
Eco47I GGWCC 1 cut(s) 162
Eco57I CTGAAG 2 cut(s) 64, 573
Eco88I CYCGRG 2 cut(s) 332, 428
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 934
EcoT14I CCWWGG 1 cut(s) 488
EcoT22I ATGCAT 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 488
FaeI CATG 5 cut(s) 406, 653, 715, 830, 875
FalI AAGNNNNNCTT 2 cut(s) 900, 932
FaqI GGGAC 3 cut(s) 45, 77, 935
FatI CATG 5 cut(s) 402, 649, 711, 826, 871
FauI CCCGC 3 cut(s) 166, 620, 626
FbaI TGATCA 3 cut(s) 421, 652, 829
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 8 cut(s) 181, 319, 386, 622, 625, 669, 689, 837
FokI GGATG 1 cut(s) 446
Fsp4HI GCNGC 8 cut(s) 181, 319, 386, 622, 625, 669, 689, 837
GluI GCNGC 8 cut(s) 181, 319, 386, 622, 625, 669, 689, 837
GsaI CCCAGC 2 cut(s) 64, 843
GsuI CTGGAG 1 cut(s) 681
HaeIII GGCC 1 cut(s) 245
HapII CCGG 7 cut(s) 29, 242, 290, 309, 315, 382, 802
Hin1II CATG 5 cut(s) 406, 653, 715, 830, 875
HinfI GANTC 4 cut(s) 17, 33, 378, 633
HpaII CCGG 7 cut(s) 29, 242, 290, 309, 315, 382, 802
HphI GGTGA 2 cut(s) 28, 364
Hpy166II GTNNAC 2 cut(s) 300, 507
Hpy188I TCNGA 5 cut(s) 154, 166, 238, 257, 553
Hpy188III TCNNGA 8 cut(s) 92, 212, 332, 403, 428, 650, 827, 872
Hpy8I GTNNAC 2 cut(s) 300, 507
Hpy99I CGWCG 4 cut(s) 140, 158, 568, 580
HpyAV CCTTC 5 cut(s) 343, 358, 457, 578, 902
HpyCH4III ACNGT 9 cut(s) 68, 103, 207, 443, 526, 568, 596, 607, 772
HpyCH4IV ACGT 2 cut(s) 544, 578
HpyCH4V TGCA 6 cut(s) 507, 522, 611, 688, 715, 855
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 382, 513, 712
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 2 cut(s) 544, 578
Hsp92II CATG 5 cut(s) 406, 653, 715, 830, 875
Ksp22I TGATCA 3 cut(s) 421, 652, 829
KspI CCGCGG 1 cut(s) 622
LmnI GCTCC 4 cut(s) 155, 311, 317, 492
Lsp1109I GCAGC 4 cut(s) 192, 397, 700, 848
MaeII ACGT 2 cut(s) 544, 578
MaeIII GTNAC 1 cut(s) 92
MboII GAAGA 3 cut(s) 70, 541, 566
MflI RGATCY 4 cut(s) 257, 283, 556, 845
MhlI GDGCHC 1 cut(s) 509
MluCI AATT 6 cut(s) 113, 124, 570, 729, 747, 763
MlyI GAGTC 3 cut(s) 11, 27, 642
MmeI TCCRAC 6 cut(s) 177, 235, 261, 584, 746, 779
MnlI CCTC 7 cut(s) 274, 288, 339, 424, 559, 770, 942
Mph1103I ATGCAT 1 cut(s) 717
MroXI GAANNNNTTC 2 cut(s) 207, 912
MseI TTAA 3 cut(s) 471, 683, 861
MspA1I CMGCKG 2 cut(s) 621, 839
MspI CCGG 7 cut(s) 29, 242, 290, 309, 315, 382, 802
MspR9I CCNGG 4 cut(s) 30, 242, 803, 936
Mva1269I GAATGC 1 cut(s) 945
MvaI CCWGG 1 cut(s) 936
MvnI CGCG 3 cut(s) 24, 200, 621
MwoI GCNNNNNNNGC 4 cut(s) 318, 382, 513, 712
NciI CCSGG 3 cut(s) 30, 242, 803
NlaIII CATG 5 cut(s) 406, 653, 715, 830, 875
NlaIV GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 847
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 92
NsiI ATGCAT 1 cut(s) 717
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 4 cut(s) 402, 649, 826, 871
PcsI WCGNNNNNNNCGW 3 cut(s) 153, 162, 572
PctI GAATGC 1 cut(s) 945
PdmI GAANNNNTTC 2 cut(s) 207, 912
PfeI GAWTC 1 cut(s) 378
PfoI TCCNGGA 2 cut(s) 28, 934
PkrI GCNGC 8 cut(s) 182, 320, 387, 623, 626, 670, 690, 838
Ple19I CGATCG 1 cut(s) 91
PleI GAGTC 3 cut(s) 11, 27, 641
PpsI GAGTC 3 cut(s) 11, 27, 641
PshBI ATTAAT 1 cut(s) 861
Psp1406I AACGTT 1 cut(s) 544
Psp6I CCWGG 1 cut(s) 934
PspFI CCCAGC 2 cut(s) 60, 839
PspGI CCWGG 1 cut(s) 934
PspN4I GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 847
PspPI GGNCC 2 cut(s) 162, 244
PsrI GAACNNNNNNTAC 2 cut(s) 879, 911
PsuI RGATCY 4 cut(s) 257, 283, 556, 845
PvuI CGATCG 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 839
RsaI GTAC 4 cut(s) 105, 814, 891, 930
RsaNI GTAC 4 cut(s) 104, 813, 890, 929
SacII CCGCGG 1 cut(s) 622
SaqAI TTAA 3 cut(s) 471, 683, 861
SatI GCNGC 8 cut(s) 181, 319, 386, 622, 625, 669, 689, 837
Sau96I GGNCC 2 cut(s) 162, 244
SchI GAGTC 3 cut(s) 11, 27, 642
ScrFI CCNGG 4 cut(s) 30, 242, 803, 936
SduI GDGCHC 1 cut(s) 509
SfcI CTRYAG 2 cut(s) 276, 900
Sfr274I CTCGAG 1 cut(s) 428
Sfr303I CCGCGG 1 cut(s) 622
SgrBI CCGCGG 1 cut(s) 622
SinI GGWCC 1 cut(s) 162
SlaI CTCGAG 1 cut(s) 428
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 6 cut(s) 113, 124, 570, 729, 747, 763
SspI AATATT 1 cut(s) 640
StyD4I CCNGG 4 cut(s) 28, 240, 801, 934
StyI CCWWGG 1 cut(s) 488
TaaI ACNGT 9 cut(s) 68, 103, 207, 443, 526, 568, 596, 607, 772
TaiI ACGT 2 cut(s) 547, 581
TaqI TCGA 2 cut(s) 429, 954
TasI AATT 6 cut(s) 113, 124, 570, 729, 747, 763
TauI GCSGC 4 cut(s) 321, 624, 627, 671
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 3 cut(s) 471, 683, 861
Tru9I TTAA 3 cut(s) 471, 683, 861
TscAI CASTG 3 cut(s) 529, 599, 857
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 4 cut(s) 180, 385, 688, 836
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 4 cut(s) 391, 597, 700, 921
TspGWI ACGGA 1 cut(s) 939
TspRI CASTG 3 cut(s) 529, 599, 857
VneI GTGCAC 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 162
VspI ATTAAT 1 cut(s) 861
XapI RAATTY 2 cut(s) 113, 124
XhoI CTCGAG 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 299
XmnI GAANNNNTTC 2 cut(s) 207, 912
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.