RchiOBHm_Chr4g0438661
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0006868
Physical Location & Seq
Reverse (-)
61288130 .. 61291331
3202 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40678

Sequence Viewer

Length: 969 bp
ATGGACGGCGACAAGGACACACGCGATCCGGGACTCACCGAGTTCCCCACCGAGCCGAGCTGGGACAGTCCTTACTTCTTCAGCAACGATCGTGACACCACCGTACTAAGCGAATTCGGGTGGAATTTCAGCCCCGACCACCAGATCGGCGCCGACGACAGGTCCGATTTGGCGGGAAGCAGCACTACTACTACTCTCGCGGAACAGCTTCCTGAAAGAAGCAGTAATACTAACTCCGACCCGGCCCCCGTGGTCGGATCTGTTTCGTCAAGCTCTACAGAGGATCCACCGGAGAGGTCTACTGGCTCCGGCTCCGGCGGCACACCACCTCCCGAGACACCGAGTAGCACTACGAAGGTGAATAAAAAGAAGGGGCAGAAGCGAATCCGGCAGCCAAGATTTGCGTTTATGACAAAGAGTGAAGTTGATCATCTCGAGGATGGCTACCGTTGGCGGAAATATGGACAGAAGGCTGTTAAGAATAGCCCATATCCAAGGAGCTACTATCGGTGCACAAATAGCAAATGCACTGTGAAGAAAAGGGTTGAACGTTCCTCTGAAGATCCAACGACGGTAATTACGACGTATGAAGGGCAACACTGTCATCATACGGTTGCATTTCCCCGCGGCGGCGGGGGAGTCAATATTATTAGTCATGATCAATCTGGCTTTGCCGCAGGAAGCCAGTTAATGCAGCTTCCTCCAGTCTCGCAGTTCATGCATTATCCAACTTCAATTCAACCCAGATCACAAATTAGTCCAACTCATAATTTGACAGTAGGAGGGTCGCAGTCGCACCGATTAGTCCGGGCTGGCGGTACTCATTACCATCATGATCAAGCAGCTGGGGGATCCCAGTGCATTATTAATCCAAATCATGAGCCACTCCGAGTAGTACCAGTTCCTACAGATGAAGGGCTTCTTGGGGACATTGTACCTCCTGGAATGCGTAATCCGTTCGATGGATAA

Protein Analysis

322

Amino Acids

35.17

Weight (kDa)

6.49

Isoelectric Point (pI)

65.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 146 - 202 1e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 149
AccI GTMKAC 1 cut(s) 299
AccII CGCG 3 cut(s) 24, 200, 627
AclI AACGTT 1 cut(s) 550
AclWI GGATC 7 cut(s) 20, 265, 278, 291, 557, 846, 859
AcsI RAATTY 2 cut(s) 113, 124
AcuI CTGAAG 2 cut(s) 64, 579
AcyI GRCGYC 1 cut(s) 150
AfaI GTAC 4 cut(s) 105, 820, 897, 936
AfiI CCNNNNNNNGG 4 cut(s) 159, 254, 629, 962
AgsI TTSAA 3 cut(s) 548, 735, 740
AhdI GACNNNNNGTC 1 cut(s) 160
AjnI CCWGG 1 cut(s) 940
AjuI GAANNNNNNNTTGG 2 cut(s) 906, 938
AluBI AGCT 6 cut(s) 60, 208, 273, 501, 697, 845
AluI AGCT 6 cut(s) 60, 208, 273, 501, 697, 845
Alw21I GWGCWC 1 cut(s) 515
Alw26I GTCTC 2 cut(s) 329, 712
Alw44I GTGCAC 1 cut(s) 511
AlwI GGATC 7 cut(s) 20, 265, 278, 291, 557, 846, 859
Ama87I CYCGRG 2 cut(s) 332, 434
AoxI GGCC 1 cut(s) 243
ApaLI GTGCAC 1 cut(s) 511
ApeKI GCWGC 4 cut(s) 180, 391, 694, 842
ApoI RAATTY 2 cut(s) 113, 124
AseI ATTAAT 1 cut(s) 867
Asp700I GAANNNNTTC 2 cut(s) 207, 918
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 162, 244
AsuC2I CCSGG 3 cut(s) 30, 242, 809
AsuHPI GGTGA 2 cut(s) 28, 370
AvaI CYCGRG 2 cut(s) 332, 434
AvaII GGWCC 1 cut(s) 162
BaeGI GKGCMC 1 cut(s) 515
BamHI GGATCC 2 cut(s) 283, 851
BanI GGYRCC 1 cut(s) 149
BarI GAAGNNNNNNTAC 4 cut(s) 169, 201, 211, 243
Bbv12I GWGCWC 1 cut(s) 515
BbvI GCAGC 4 cut(s) 192, 403, 706, 854
BccI CCATC 3 cut(s) 434, 837, 956
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 942
BclI TGATCA 3 cut(s) 427, 658, 835
BcnI CCSGG 3 cut(s) 30, 242, 809
BcoDI GTCTC 2 cut(s) 329, 712
BfmI CTRYAG 2 cut(s) 276, 906
BfoI RGCGCY 1 cut(s) 153
BisI GCNGC 8 cut(s) 181, 319, 392, 628, 631, 675, 695, 843
BlsI GCNGC 8 cut(s) 182, 320, 393, 629, 632, 676, 696, 844
Bme1390I CCNGG 4 cut(s) 30, 242, 809, 942
Bme18I GGWCC 1 cut(s) 162
BmeRI GACNNNNNGTC 1 cut(s) 160
BmeT110I CYCGRG 2 cut(s) 332, 434
BmgT120I GGNCC 2 cut(s) 162, 244
BmiI GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 853
BmrFI CCNGG 4 cut(s) 30, 242, 809, 942
BmrI ACTGGG 1 cut(s) 850
BmuI ACTGGG 1 cut(s) 850
BpmI CTGGAG 1 cut(s) 687
BpuMI CCSGG 3 cut(s) 30, 242, 809
BsaHI GRCGYC 1 cut(s) 150
BsaJI CCNNGG 3 cut(s) 249, 494, 625
BsaWI WCCGGW 1 cut(s) 289
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 4 cut(s) 159, 254, 629, 962
Bse1I ACTGG 5 cut(s) 307, 685, 704, 856, 899
BseBI CCWGG 1 cut(s) 942
BseDI CCNNGG 3 cut(s) 249, 494, 625
BseGI GGATG 1 cut(s) 445
BseLI CCNNNNNNNGG 4 cut(s) 159, 254, 629, 962
BseNI ACTGG 5 cut(s) 307, 685, 704, 856, 899
BseSI GKGCMC 1 cut(s) 515
BseXI GCAGC 4 cut(s) 192, 403, 706, 854
BseYI CCCAGC 2 cut(s) 60, 845
Bsh1236I CGCG 3 cut(s) 24, 200, 627
Bsh1285I CGRYCG 1 cut(s) 91
BshFI GGCC 1 cut(s) 245
BshNI GGYRCC 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 515
BsiHKCI CYCGRG 2 cut(s) 332, 434
BsiSI CCGG 7 cut(s) 29, 242, 290, 309, 315, 388, 808
BslFI GGGAC 3 cut(s) 45, 77, 941
BslI CCNNNNNNNGG 4 cut(s) 159, 254, 629, 962
BsmAI GTCTC 2 cut(s) 329, 712
BsmFI GGGAC 3 cut(s) 45, 77, 941
BsmI GAATGC 1 cut(s) 951
BsnI GGCC 1 cut(s) 245
BsoBI CYCGRG 2 cut(s) 332, 434
Bsp1286I GDGCHC 1 cut(s) 515
BspANI GGCC 1 cut(s) 245
BspFNI CGCG 3 cut(s) 24, 200, 627
BspHI TCATGA 3 cut(s) 655, 832, 877
BspLI GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 853
BspPI GGATC 7 cut(s) 20, 265, 278, 291, 557, 846, 859
BspT107I GGYRCC 1 cut(s) 149
BsrI ACTGG 5 cut(s) 307, 685, 704, 856, 899
BssECI CCNNGG 3 cut(s) 249, 494, 625
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 1 cut(s) 494
Bst2UI CCWGG 1 cut(s) 942
Bst4CI ACNGT 8 cut(s) 68, 103, 449, 532, 574, 602, 613, 778
BstACI GRCGYC 1 cut(s) 150
BstAPI GCANNNNNTGC 1 cut(s) 718
BstC8I GCNNGC 1 cut(s) 814
BstDEI CTNAG 1 cut(s) 107
BstDSI CCRYGG 2 cut(s) 249, 625
BstF5I GGATG 1 cut(s) 445
BstFNI CGCG 3 cut(s) 24, 200, 627
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstMAI GTCTC 2 cut(s) 329, 712
BstMCI CGRYCG 1 cut(s) 91
BstMWI GCNNNNNNNGC 4 cut(s) 318, 388, 519, 718
BstNI CCWGG 1 cut(s) 942
BstSCI CCNGG 4 cut(s) 28, 240, 807, 940
BstSFI CTRYAG 2 cut(s) 276, 906
BstSLI GKGCMC 1 cut(s) 515
BstUI CGCG 3 cut(s) 24, 200, 627
BstV1I GCAGC 4 cut(s) 192, 403, 706, 854
BstX2I RGATCY 4 cut(s) 257, 283, 562, 851
BstYI RGATCY 4 cut(s) 257, 283, 562, 851
BsuRI GGCC 1 cut(s) 245
BtgI CCRYGG 2 cut(s) 249, 625
BtsCI GGATG 1 cut(s) 445
BtsIMutI CAGTG 3 cut(s) 528, 598, 863
Cac8I GCNNGC 1 cut(s) 814
CciI TCATGA 3 cut(s) 655, 832, 877
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 162, 244
Cfr42I CCGCGG 1 cut(s) 628
Csp6I GTAC 4 cut(s) 104, 819, 896, 935
CviAII CATG 4 cut(s) 656, 718, 833, 878
CviQI GTAC 4 cut(s) 104, 819, 896, 935
DdeI CTNAG 1 cut(s) 107
DinI GGCGCC 1 cut(s) 151
DriI GACNNNNNGTC 1 cut(s) 160
Eam1105I GACNNNNNGTC 1 cut(s) 160
EciI GGCGGA 1 cut(s) 469
Eco130I CCWWGG 1 cut(s) 494
Eco47I GGWCC 1 cut(s) 162
Eco57I CTGAAG 2 cut(s) 64, 579
Eco88I CYCGRG 2 cut(s) 332, 434
EcoRI GAATTC 1 cut(s) 113
EcoRII CCWGG 1 cut(s) 940
EcoT14I CCWWGG 1 cut(s) 494
EcoT22I ATGCAT 1 cut(s) 723
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 494
FaeI CATG 4 cut(s) 659, 721, 836, 881
FalI AAGNNNNNCTT 2 cut(s) 906, 938
FaqI GGGAC 3 cut(s) 45, 77, 941
FatI CATG 4 cut(s) 655, 717, 832, 877
FauI CCCGC 3 cut(s) 166, 626, 632
FbaI TGATCA 3 cut(s) 427, 658, 835
FblI GTMKAC 1 cut(s) 299
Fnu4HI GCNGC 8 cut(s) 181, 319, 392, 628, 631, 675, 695, 843
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 8 cut(s) 181, 319, 392, 628, 631, 675, 695, 843
GlaI GCGC 1 cut(s) 151
GluI GCNGC 8 cut(s) 181, 319, 392, 628, 631, 675, 695, 843
GsaI CCCAGC 2 cut(s) 64, 849
GsuI CTGGAG 1 cut(s) 687
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 1 cut(s) 245
HapII CCGG 7 cut(s) 29, 242, 290, 309, 315, 388, 808
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 4 cut(s) 659, 721, 836, 881
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 3 cut(s) 33, 384, 639
HpaII CCGG 7 cut(s) 29, 242, 290, 309, 315, 388, 808
HphI GGTGA 2 cut(s) 28, 370
Hpy166II GTNNAC 2 cut(s) 300, 513
Hpy188I TCNGA 5 cut(s) 166, 238, 257, 559, 890
Hpy188III TCNNGA 7 cut(s) 92, 212, 332, 434, 656, 833, 878
Hpy8I GTNNAC 2 cut(s) 300, 513
Hpy99I CGWCG 3 cut(s) 158, 574, 586
HpyAV CCTTC 5 cut(s) 349, 364, 463, 584, 908
HpyCH4III ACNGT 8 cut(s) 68, 103, 449, 532, 574, 602, 613, 778
HpyCH4IV ACGT 2 cut(s) 550, 584
HpyCH4V TGCA 6 cut(s) 513, 528, 617, 694, 721, 861
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 388, 519, 718
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 2 cut(s) 550, 584
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 4 cut(s) 659, 721, 836, 881
HspAI GCGC 1 cut(s) 150
KasI GGCGCC 1 cut(s) 149
Ksp22I TGATCA 3 cut(s) 427, 658, 835
KspI CCGCGG 1 cut(s) 628
LmnI GCTCC 3 cut(s) 311, 317, 498
Lsp1109I GCAGC 4 cut(s) 192, 403, 706, 854
MaeII ACGT 2 cut(s) 550, 584
MaeIII GTNAC 1 cut(s) 92
MboII GAAGA 3 cut(s) 70, 547, 572
MflI RGATCY 4 cut(s) 257, 283, 562, 851
MhlI GDGCHC 1 cut(s) 515
MluCI AATT 6 cut(s) 113, 124, 576, 735, 753, 769
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 2 cut(s) 27, 648
MmeI TCCRAC 5 cut(s) 235, 261, 590, 752, 785
MnlI CCTC 8 cut(s) 274, 288, 339, 430, 565, 711, 776, 948
Mph1103I ATGCAT 1 cut(s) 723
MroXI GAANNNNTTC 2 cut(s) 207, 918
MseI TTAA 3 cut(s) 477, 689, 867
MspA1I CMGCKG 2 cut(s) 627, 845
MspI CCGG 7 cut(s) 29, 242, 290, 309, 315, 388, 808
MspR9I CCNGG 4 cut(s) 30, 242, 809, 942
Mva1269I GAATGC 1 cut(s) 951
MvaI CCWGG 1 cut(s) 942
MvnI CGCG 3 cut(s) 24, 200, 627
MwoI GCNNNNNNNGC 4 cut(s) 318, 388, 519, 718
NarI GGCGCC 1 cut(s) 150
NciI CCSGG 3 cut(s) 30, 242, 809
NlaIII CATG 4 cut(s) 659, 721, 836, 881
NlaIV GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 853
NmeAIII GCCGAG 1 cut(s) 81
NmuCI GTSAC 1 cut(s) 92
NsiI ATGCAT 1 cut(s) 723
PaeR7I CTCGAG 1 cut(s) 434
PagI TCATGA 3 cut(s) 655, 832, 877
PcsI WCGNNNNNNNCGW 3 cut(s) 153, 162, 578
PctI GAATGC 1 cut(s) 951
PdmI GAANNNNTTC 2 cut(s) 207, 918
PfeI GAWTC 1 cut(s) 384
PfoI TCCNGGA 2 cut(s) 28, 940
PkrI GCNGC 8 cut(s) 182, 320, 393, 629, 632, 676, 696, 844
Ple19I CGATCG 1 cut(s) 91
PleI GAGTC 2 cut(s) 27, 647
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 2 cut(s) 27, 647
PshBI ATTAAT 1 cut(s) 867
Psp1406I AACGTT 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 940
PspFI CCCAGC 2 cut(s) 60, 845
PspGI CCWGG 1 cut(s) 940
PspN4I GGNNCC 6 cut(s) 151, 246, 285, 307, 313, 853
PspPI GGNCC 2 cut(s) 162, 244
PsrI GAACNNNNNNTAC 2 cut(s) 885, 917
PsuI RGATCY 4 cut(s) 257, 283, 562, 851
PvuI CGATCG 1 cut(s) 91
PvuII CAGCTG 1 cut(s) 845
RsaI GTAC 4 cut(s) 105, 820, 897, 936
RsaNI GTAC 4 cut(s) 104, 819, 896, 935
SacII CCGCGG 1 cut(s) 628
SaqAI TTAA 3 cut(s) 477, 689, 867
SatI GCNGC 8 cut(s) 181, 319, 392, 628, 631, 675, 695, 843
Sau96I GGNCC 2 cut(s) 162, 244
SchI GAGTC 2 cut(s) 27, 648
ScrFI CCNGG 4 cut(s) 30, 242, 809, 942
SduI GDGCHC 1 cut(s) 515
SfcI CTRYAG 2 cut(s) 276, 906
SfoI GGCGCC 1 cut(s) 151
Sfr274I CTCGAG 1 cut(s) 434
Sfr303I CCGCGG 1 cut(s) 628
SgrBI CCGCGG 1 cut(s) 628
SinI GGWCC 1 cut(s) 162
SlaI CTCGAG 1 cut(s) 434
SmlI CTYRAG 1 cut(s) 434
SmoI CTYRAG 1 cut(s) 434
Sse9I AATT 6 cut(s) 113, 124, 576, 735, 753, 769
SspDI GGCGCC 1 cut(s) 149
SspI AATATT 1 cut(s) 646
StyD4I CCNGG 4 cut(s) 28, 240, 807, 940
StyI CCWWGG 1 cut(s) 494
TaaI ACNGT 8 cut(s) 68, 103, 449, 532, 574, 602, 613, 778
TaiI ACGT 2 cut(s) 553, 587
TaqI TCGA 2 cut(s) 435, 960
TasI AATT 6 cut(s) 113, 124, 576, 735, 753, 769
TauI GCSGC 4 cut(s) 321, 630, 633, 677
TfiI GAWTC 1 cut(s) 384
Tru1I TTAA 3 cut(s) 477, 689, 867
Tru9I TTAA 3 cut(s) 477, 689, 867
TscAI CASTG 3 cut(s) 535, 605, 863
TseFI GTSAC 1 cut(s) 92
TseI GCWGC 4 cut(s) 180, 391, 694, 842
Tsp45I GTSAC 1 cut(s) 92
TspDTI ATGAA 3 cut(s) 603, 706, 927
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 3 cut(s) 535, 605, 863
VneI GTGCAC 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 162
VspI ATTAAT 1 cut(s) 867
XapI RAATTY 2 cut(s) 113, 124
XhoI CTCGAG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 299
XmnI GAANNNNTTC 2 cut(s) 207, 918
Zsp2I ATGCAT 1 cut(s) 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.