MD13G1064700.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0006868
Physical Location & Seq
Reverse (-)
4465565 .. 4471604
6040 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1064700.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGGACGACAACAACCAAAAACGCGATCCACTAACCACGACCGAGTTCACGACTCACACCTGGACTCCCGACTCGGACAGCGCCTACTTCTTCAATACCCACGACAGAGACAACAACATACTCAGCGACTTCGGCTGGATTTTCAACCCGGAAGCCTCCAAACCCGATGACGGCTTCCCCTGCCTCGACCCCATCGCCGCCGCCGCCGACACGTCCGATTTGGCGGGAACTACTAGTAGTGGTCCTCTTGTTTTGGATAACAACAGCAGCAGTAGCAGCGCCGCCTTTGGGAGCCCCGACCCGGCCCCTGTTGTCGGATCCGCTTCGACCAATCCGTCAGTGTCAACGAGCTCCAGCGAGGATCCGCCTGAGAGGTCTACAGGCTCCGGTGGCAAACCACCGTCCGAGATACCGAGTAAGGTTAAAATGAAGGGGCCGAAGAGAATCCGGCAGCCACGATTCGCATTCATGACAAAGAGTGAAGTTGATAATCTTGAGGATGGCTACCGTTGGCGGAAATATGGTCAGAAGGCTGTTAAAAATAGCCCCTTTCCAAGGAGCTATTATCGCTGCACAAATACCAAATGCACAGTGAAGAAAAGAGTTGAGCGTTCCTCTCAAGATCCCACAACTGTAATTACCACATATGAAGGCCAACACTGTCATCATACAGTTGCATTCCCTCGTGGGGGAGTCATGGGTCATGAATCTGGCTTTACAGGCCACCAGTTGTCTCAGCTGCCTCCAGCCTCACAGTTCATGTATTATCCAACAATTCGACCGAGAGAGATAACTAGTCCTATTAATTTGACCCCAACCTCGTCGCACCAAGTAGTAGTCCCACGTCATGACGATGATCAAGCTGCAGGAGGATCATCCCACTCCACTGACCGTCAGCCAACCCCACCTCTTCCTACAGACGAAGGGCTTCTTGGAGACGTTGGCCGTAGGATCAATATTGTTAAATTAGCTTGGCCTACGTCGTCTCTACAGGCCATGCATGCGGGTAGGCTTAGCAGCTGCATTATCTTCAGCTTCTGA

Protein Analysis

347

Amino Acids

37.73

Weight (kDa)

6.59

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 166 - 222 6.4e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 334
AccI GTMKAC 1 cut(s) 377
AccII CGCG 1 cut(s) 24
AciI CCGC 9 cut(s) 198, 201, 204, 224, 282, 321, 365, 514, 1004
AclWI GGATC 8 cut(s) 20, 312, 325, 356, 369, 617, 880, 959
AcoI YGGCCR 1 cut(s) 943
AcuI CTGAAG 1 cut(s) 1015
AfiI CCNNNNNNNGG 7 cut(s) 170, 288, 301, 314, 555, 688, 689
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 2 cut(s) 94, 145
AhlI ACTAGT 2 cut(s) 233, 794
AjiI CACGTC 2 cut(s) 213, 845
AjnI CCWGG 1 cut(s) 59
AjuI GAANNNNNNNTTGG 2 cut(s) 915, 947
AluBI AGCT 7 cut(s) 351, 561, 739, 863, 971, 1020, 1035
AluI AGCT 7 cut(s) 351, 561, 739, 863, 971, 1020, 1035
Alw21I GWGCWC 1 cut(s) 353
Alw26I GTCTC 4 cut(s) 102, 738, 930, 990
AlwI GGATC 8 cut(s) 20, 312, 325, 356, 369, 617, 880, 959
AlwNI CAGNNNCTG 1 cut(s) 1038
AoxI GGCC 7 cut(s) 303, 434, 652, 721, 943, 974, 993
ApeKI GCWGC 8 cut(s) 267, 276, 451, 570, 739, 863, 1017, 1020
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 1 cut(s) 927
AspLEI GCGC 2 cut(s) 83, 281
AspS9I GGNCC 3 cut(s) 242, 304, 434
AsuC2I CCSGG 2 cut(s) 149, 302
AvaII GGWCC 1 cut(s) 242
BamHI GGATCC 2 cut(s) 317, 361
BanII GRGCYC 2 cut(s) 296, 353
BauI CACGAG 1 cut(s) 684
Bbv12I GWGCWC 1 cut(s) 353
BbvI GCAGC 8 cut(s) 279, 288, 463, 557, 726, 850, 1007, 1029
BccI CCATC 2 cut(s) 200, 494
BceAI ACGGC 2 cut(s) 187, 930
BciT130I CCWGG 1 cut(s) 61
BclI TGATCA 1 cut(s) 856
BcnI CCSGG 2 cut(s) 149, 302
BcoDI GTCTC 4 cut(s) 102, 738, 930, 990
BcuI ACTAGT 2 cut(s) 233, 794
BfaI CTAG 2 cut(s) 234, 795
BfmI CTRYAG 4 cut(s) 378, 864, 915, 989
BfoI RGCGCY 2 cut(s) 84, 282
BlpI GCTNAGC 1 cut(s) 1013
Bme1390I CCNGG 3 cut(s) 61, 149, 302
Bme18I GGWCC 1 cut(s) 242
BmgBI CACGTC 2 cut(s) 213, 845
BmgT120I GGNCC 3 cut(s) 242, 304, 434
BmiI GGNNCC 6 cut(s) 293, 306, 319, 363, 385, 435
BmrFI CCNGG 3 cut(s) 61, 149, 302
BplI GAGNNNNNCTC 2 cut(s) 599, 631
BpmI CTGGAG 2 cut(s) 337, 729
Bpu1102I GCTNAGC 1 cut(s) 1013
BpuEI CTTGAG 2 cut(s) 515, 603
BpuMI CCSGG 2 cut(s) 149, 302
BsaJI CCNNGG 1 cut(s) 554
BsaWI WCCGGW 1 cut(s) 386
BsaXI ACNNNNNCTCC 2 cut(s) 49, 79
Bsc4I CCNNNNNNNGG 7 cut(s) 170, 288, 301, 314, 555, 688, 689
Bse1I ACTGG 1 cut(s) 727
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 554
BseGI GGATG 2 cut(s) 505, 875
BseLI CCNNNNNNNGG 7 cut(s) 170, 288, 301, 314, 555, 688, 689
BseMII CTCAG 3 cut(s) 136, 360, 749
BseNI ACTGG 1 cut(s) 727
BseXI GCAGC 8 cut(s) 279, 288, 463, 557, 726, 850, 1007, 1029
BsgI GTGCAG 1 cut(s) 556
Bsh1236I CGCG 1 cut(s) 24
Bsh1285I CGRYCG 2 cut(s) 42, 782
BshFI GGCC 7 cut(s) 305, 436, 654, 723, 945, 976, 995
BsiEI CGRYCG 2 cut(s) 42, 782
BsiHKAI GWGCWC 1 cut(s) 353
BsiSI CCGG 4 cut(s) 149, 302, 387, 448
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 7 cut(s) 170, 288, 301, 314, 555, 688, 689
BsmAI GTCTC 4 cut(s) 102, 738, 930, 990
BsmBI CGTCTC 2 cut(s) 930, 990
BsmFI GGGAC 1 cut(s) 824
BsmI GAATGC 2 cut(s) 464, 677
BsnI GGCC 7 cut(s) 305, 436, 654, 723, 945, 976, 995
Bsp1286I GDGCHC 2 cut(s) 296, 353
Bsp143I GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
Bsp1720I GCTNAGC 1 cut(s) 1013
BspACI CCGC 9 cut(s) 198, 201, 204, 224, 282, 321, 365, 514, 1004
BspANI GGCC 7 cut(s) 305, 436, 654, 723, 945, 976, 995
BspCNI CTCAG 3 cut(s) 135, 361, 748
BspFNI CGCG 1 cut(s) 24
BspHI TCATGA 3 cut(s) 468, 703, 847
BspLI GGNNCC 6 cut(s) 293, 306, 319, 363, 385, 435
BspMAI CTGCAG 1 cut(s) 868
BspPI GGATC 8 cut(s) 20, 312, 325, 356, 369, 617, 880, 959
BsrI ACTGG 1 cut(s) 727
BssECI CCNNGG 1 cut(s) 554
BssMI GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
BssSI CACGAG 1 cut(s) 684
BssT1I CCWWGG 1 cut(s) 554
Bst2BI CACGAG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 8 cut(s) 402, 509, 592, 634, 662, 673, 756, 893
Bst6I CTCTTC 2 cut(s) 434, 915
BstC8I GCNNGC 1 cut(s) 1002
BstDEI CTNAG 4 cut(s) 122, 369, 735, 1013
BstENI CCTNNNNNAGG 1 cut(s) 553
BstF5I GGATG 2 cut(s) 505, 875
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 2 cut(s) 84, 282
BstHHI GCGC 2 cut(s) 83, 281
BstKTI GATC 7 cut(s) 28, 320, 364, 625, 859, 875, 954
BstMAI GTCTC 4 cut(s) 102, 738, 930, 990
BstMBI GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
BstMCI CGRYCG 2 cut(s) 42, 782
BstMWI GCNNNNNNNGC 9 cut(s) 132, 180, 203, 273, 276, 390, 567, 720, 1001
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 1 cut(s) 1004
BstSCI CCNGG 3 cut(s) 59, 147, 300
BstSFI CTRYAG 4 cut(s) 378, 864, 915, 989
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 8 cut(s) 279, 288, 463, 557, 726, 850, 1007, 1029
BstX2I RGATCY 3 cut(s) 317, 361, 622
BstYI RGATCY 3 cut(s) 317, 361, 622
BsuRI GGCC 7 cut(s) 305, 436, 654, 723, 945, 976, 995
BtgZI GCGATG 1 cut(s) 178
BtrI CACGTC 2 cut(s) 213, 845
BtsCI GGATG 2 cut(s) 505, 875
BtsIMutI CAGTG 4 cut(s) 345, 597, 658, 885
Cac8I GCNNGC 1 cut(s) 1002
CaiI CAGNNNCTG 1 cut(s) 1038
CciI TCATGA 3 cut(s) 468, 703, 847
CfoI GCGC 2 cut(s) 83, 281
Cfr13I GGNCC 3 cut(s) 242, 304, 434
CviAII CATG 7 cut(s) 469, 697, 704, 760, 848, 997, 1001
DdeI CTNAG 4 cut(s) 122, 369, 735, 1013
DpnI GATC 7 cut(s) 27, 319, 363, 624, 858, 874, 953
DpnII GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
DrdI GACNNNNNNGTC 1 cut(s) 334
DseDI GACNNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 943
Eam1104I CTCTTC 2 cut(s) 434, 915
EarI CTCTTC 2 cut(s) 434, 915
EciI GGCGGA 2 cut(s) 354, 529
Ecl136II GAGCTC 1 cut(s) 351
Eco130I CCWWGG 1 cut(s) 554
Eco24I GRGCYC 2 cut(s) 296, 353
Eco47I GGWCC 1 cut(s) 242
Eco53kI GAGCTC 1 cut(s) 351
Eco57I CTGAAG 1 cut(s) 1015
EcoICRI GAGCTC 1 cut(s) 351
EcoNI CCTNNNNNAGG 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 554
EcoT22I ATGCAT 1 cut(s) 1002
EcoT38I GRGCYC 2 cut(s) 296, 353
ErhI CCWWGG 1 cut(s) 554
Esp3I CGTCTC 2 cut(s) 930, 990
FaeI CATG 7 cut(s) 472, 700, 707, 763, 851, 1000, 1004
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 1 cut(s) 824
FatI CATG 7 cut(s) 468, 696, 703, 759, 847, 996, 1000
FauI CCCGC 2 cut(s) 217, 997
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 856
FblI GTMKAC 1 cut(s) 377
FokI GGATG 2 cut(s) 512, 862
FriOI GRGCYC 2 cut(s) 296, 353
FspBI CTAG 2 cut(s) 234, 795
GlaI GCGC 2 cut(s) 82, 280
GsuI CTGGAG 2 cut(s) 337, 729
HaeII RGCGCY 2 cut(s) 84, 282
HaeIII GGCC 7 cut(s) 305, 436, 654, 723, 945, 976, 995
HapII CCGG 4 cut(s) 149, 302, 387, 448
HhaI GCGC 2 cut(s) 83, 281
Hin1II CATG 7 cut(s) 472, 700, 707, 763, 851, 1000, 1004
Hin6I GCGC 2 cut(s) 81, 279
HinP1I GCGC 2 cut(s) 81, 279
HincII GTYRAC 1 cut(s) 345
HindII GTYRAC 1 cut(s) 345
HinfI GANTC 7 cut(s) 52, 64, 71, 444, 459, 693, 707
HpaII CCGG 4 cut(s) 149, 302, 387, 448
Hpy166II GTNNAC 3 cut(s) 48, 345, 378
Hpy188I TCNGA 6 cut(s) 76, 217, 317, 406, 528, 1040
Hpy188III TCNNGA 7 cut(s) 49, 68, 469, 494, 620, 704, 848
Hpy8I GTNNAC 3 cut(s) 48, 345, 378
Hpy99I CGWCG 2 cut(s) 826, 985
HpyAV CCTTC 4 cut(s) 424, 523, 644, 917
HpyCH4III ACNGT 8 cut(s) 402, 509, 592, 634, 662, 673, 756, 893
HpyCH4IV ACGT 4 cut(s) 212, 844, 939, 980
HpyCH4V TGCA 6 cut(s) 573, 588, 677, 866, 1000, 1023
HpyF10VI GCNNNNNNNGC 9 cut(s) 132, 180, 203, 273, 276, 390, 567, 720, 1001
HpyF3I CTNAG 4 cut(s) 122, 369, 735, 1013
HpySE526I ACGT 4 cut(s) 212, 844, 939, 980
Hsp92II CATG 7 cut(s) 472, 700, 707, 763, 851, 1000, 1004
HspAI GCGC 2 cut(s) 81, 279
Ksp22I TGATCA 1 cut(s) 856
Kzo9I GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
LmnI GCTCC 4 cut(s) 291, 356, 389, 558
Lsp1109I GCAGC 8 cut(s) 279, 288, 463, 557, 726, 850, 1007, 1029
MaeI CTAG 2 cut(s) 234, 795
MaeII ACGT 4 cut(s) 212, 844, 939, 980
MalI GATC 7 cut(s) 27, 319, 363, 624, 858, 874, 953
MboI GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
MboII GAAGA 5 cut(s) 82, 451, 607, 902, 1021
MflI RGATCY 3 cut(s) 317, 361, 622
MhlI GDGCHC 2 cut(s) 296, 353
MluCI AATT 4 cut(s) 636, 774, 805, 965
MlyI GAGTC 4 cut(s) 46, 58, 65, 702
MmeI TCCRAC 2 cut(s) 295, 794
Mph1103I ATGCAT 1 cut(s) 1002
MroXI GAANNNNTTC 1 cut(s) 927
MseI TTAA 4 cut(s) 423, 537, 804, 963
MslI CAYNNNNRTG 1 cut(s) 852
MspA1I CMGCKG 2 cut(s) 739, 1020
MspI CCGG 4 cut(s) 149, 302, 387, 448
MspR9I CCNGG 3 cut(s) 61, 149, 302
Mva1269I GAATGC 2 cut(s) 464, 677
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 9 cut(s) 132, 180, 203, 273, 276, 390, 567, 720, 1001
NciI CCSGG 2 cut(s) 149, 302
NdeI CATATG 1 cut(s) 646
NdeII GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
NlaIII CATG 7 cut(s) 472, 700, 707, 763, 851, 1000, 1004
NlaIV GGNNCC 6 cut(s) 293, 306, 319, 363, 385, 435
NsiI ATGCAT 1 cut(s) 1002
NspI RCATGY 1 cut(s) 1004
PaeI GCATGC 1 cut(s) 1004
PagI TCATGA 3 cut(s) 468, 703, 847
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PctI GAATGC 2 cut(s) 464, 677
PdmI GAANNNNTTC 1 cut(s) 927
PfeI GAWTC 3 cut(s) 444, 459, 707
PleI GAGTC 4 cut(s) 46, 58, 65, 701
PpsI GAGTC 4 cut(s) 46, 58, 65, 701
PshBI ATTAAT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 353
Psp6I CCWGG 1 cut(s) 59
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 6 cut(s) 293, 306, 319, 363, 385, 435
PspPI GGNCC 3 cut(s) 242, 304, 434
PstI CTGCAG 1 cut(s) 868
PstNI CAGNNNCTG 1 cut(s) 1038
PsuI RGATCY 3 cut(s) 317, 361, 622
PvuII CAGCTG 2 cut(s) 739, 1020
RseI CAYNNNNRTG 1 cut(s) 852
SacI GAGCTC 1 cut(s) 353
SaqAI TTAA 4 cut(s) 423, 537, 804, 963
Sau3AI GATC 7 cut(s) 25, 317, 361, 622, 856, 872, 951
Sau96I GGNCC 3 cut(s) 242, 304, 434
SchI GAGTC 4 cut(s) 46, 58, 65, 702
ScrFI CCNGG 3 cut(s) 61, 149, 302
SduI GDGCHC 2 cut(s) 296, 353
SfcI CTRYAG 4 cut(s) 378, 864, 915, 989
SinI GGWCC 1 cut(s) 242
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 2 cut(s) 494, 618
SmoI CTYRAG 2 cut(s) 494, 618
SpeI ACTAGT 2 cut(s) 233, 794
SphI GCATGC 1 cut(s) 1004
Sse9I AATT 4 cut(s) 636, 774, 805, 965
SsiI CCGC 9 cut(s) 198, 201, 204, 224, 282, 321, 365, 514, 1004
SspI AATATT 1 cut(s) 958
SspMI CTAG 2 cut(s) 234, 795
SstI GAGCTC 1 cut(s) 353
StyD4I CCNGG 3 cut(s) 59, 147, 300
StyI CCWWGG 1 cut(s) 554
TaaI ACNGT 8 cut(s) 402, 509, 592, 634, 662, 673, 756, 893
TaiI ACGT 4 cut(s) 215, 847, 942, 983
TaqI TCGA 3 cut(s) 186, 326, 778
TaqII GACCGA 2 cut(s) 56, 796
TasI AATT 4 cut(s) 636, 774, 805, 965
TauI GCSGC 4 cut(s) 200, 203, 206, 284
TfiI GAWTC 3 cut(s) 444, 459, 707
Tru1I TTAA 4 cut(s) 423, 537, 804, 963
Tru9I TTAA 4 cut(s) 423, 537, 804, 963
TscAI CASTG 4 cut(s) 345, 597, 665, 892
TseI GCWGC 8 cut(s) 267, 276, 451, 570, 739, 863, 1017, 1020
TspDTI ATGAA 5 cut(s) 443, 457, 663, 720, 748
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 4 cut(s) 345, 597, 665, 892
VpaK11BI GGWCC 1 cut(s) 242
VspI ATTAAT 1 cut(s) 804
XagI CCTNNNNNAGG 1 cut(s) 553
XceI RCATGY 1 cut(s) 1004
XmiI GTMKAC 1 cut(s) 377
XmnI GAANNNNTTC 1 cut(s) 927
XspI CTAG 2 cut(s) 234, 795
Zsp2I ATGCAT 1 cut(s) 1002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.