Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0006646.1

Orthogroup ID

OG0006868

Physical Location & Seq

Genomic Coordinates Forward (+)

1 .. 4749

Gene Structure 4749 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0006646.1_g000001.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1138 bp

ctttccattccctccttcaaaaactactacgcgtcctttccatgtcaccaaaaccctaattactaattaccatctctctcgccgacgcctaccttcttctaattcatgctcgcttcgccctcccaagtccaatccactctacgacgtcgtccgatccacctccgcctccatggacggcgacaaggacacacgcgatccgggactcaccgacttccccaccgagccgagctgggacagtccttacttcttcagcaacgatcgtgacaccaccgtactaagcgaattcgggtggaatttcagccccgacgaccagatcggcgccgacgacaggtccgatttggcgggaagcagcactactactactctcgcggaacagtttccagaaagaagcagtaatactaactccgacccggcccccgtggtcggatctgtttcgtcaagctctacagaggatccgccggagaggtctactggctccggctccggcggcacaccacctcccgagacaccgagtagcactacgaaggtgaataaaaagaaggggcagaagcgaatccggcagccaagatttgcgtttatgactaagagtgaagttgatcatctcgaggatggctaccgttggcggaaatatggacagaaggctgttaagaatagcccatatccaaggagctactatcggtgcacaaatagcaaatgcactgtgaagaaaagggttgaacgttcctctgaagatccaacgacggtaattacgacgtatgaagggcaacactgtcatcataccgttgcatttccccgcggcggcgggggagtgaatattattagtcatgatcaatctggctttgccgcaggaagccagttaatgcagcttcctccagttgcgcagttcatgcattatccaacttcaattcaacccagatcacaaattagtccaactcataatttgacagtaggagggtcgcagtcgcaccgattagtccgggctggcggtactcattaccatcatgatcaagcagctgggggatcccagtgcattattaatccaaatcatgagccactcccagtagtaccagttcctacagatgaagggcttcttggggacattgtacctcctggaatgcgtaatccgttcgatggataa

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001067 GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0006950 GO:0006970 GO:0008150 GO:0009414 GO:0009415 GO:0009628 GO:0009651 GO:0009889 GO:0010035 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042221 GO:0043565 GO:0044212 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

378

Amino Acids

41.42

Weight (kDa)

8.52

Isoelectric Point (pI)

69.16

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006868)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69310 AT1G69310 AT1G69310 AT1G69310
fragaria_vesca	FvH4_4g30360 FvH4_4g30360 FvH4_4g30360 FvH4_4g30360 FvH4_4g30360
malus_domestica	MD13G1064700.v1.1 MD16G1063200.v1.1
prunus_persica	Prupe.1G283500_v2.0.a1
pyrus_communis	pycom16g05540
rosa_chinensis	RchiOBHm_Chr4g0438661
rosa_laevigata	RLG00000006343
rosa_multiflora	Rmu_co8519545.1_g000001 Rmu_sc0006646.1_g000001 Rmu_sc0006646.1_g000002
rosa_roxburghii	Rroxscaffold_5G00379660
rosa_rugosa	Rorug04G0312400
rosa_samantha	Rh4AG362800 Rh4BG374100 Rh4CG388100 Rh4DG368800
rosa_wichuraiana	Rw0G007130 Rw0G016680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	148
Acc16I	TGCGCA	1 cut(s)	880
AccB1I	GGYRCC	1 cut(s)	318
AccI	GTMKAC	1 cut(s)	468
AccII	CGCG	4 cut(s)	32, 193, 369, 796
AclI	AACGTT	1 cut(s)	719
AclWI	GGATC	8 cut(s)	148, 189, 434, 447, 460, 726, 1015, 1028
AcsI	RAATTY	2 cut(s)	282, 293
AcuI	CTGAAG	2 cut(s)	233, 748
AcyI	GRCGYC	3 cut(s)	86, 145, 319
AfaI	GTAC	4 cut(s)	274, 989, 1066, 1105
AfiI	CCNNNNNNNGG	4 cut(s)	328, 423, 798, 1131
AflIII	ACRYGT	1 cut(s)	30
AgsI	TTSAA	4 cut(s)	19, 717, 904, 909
AhdI	GACNNNNNGTC	1 cut(s)	329
AjnI	CCWGG	1 cut(s)	1109
AjuI	GAANNNNNNNTTGG	2 cut(s)	1075, 1107
AluBI	AGCT	5 cut(s)	229, 442, 670, 866, 1014
AluI	AGCT	5 cut(s)	229, 442, 670, 866, 1014
Alw21I	GWGCWC	1 cut(s)	684
Alw26I	GTCTC	1 cut(s)	498
Alw44I	GTGCAC	1 cut(s)	680
AlwI	GGATC	8 cut(s)	148, 189, 434, 447, 460, 726, 1015, 1028
Ama87I	CYCGRG	2 cut(s)	501, 603
AoxI	GGCC	1 cut(s)	412
ApaLI	GTGCAC	1 cut(s)	680
ApeKI	GCWGC	4 cut(s)	349, 560, 863, 1011
ApoI	RAATTY	2 cut(s)	282, 293
AseI	ATTAAT	1 cut(s)	1036
Asp700I	GAANNNNTTC	2 cut(s)	376, 1087
AspLEI	GCGC	2 cut(s)	321, 881
AspS9I	GGNCC	2 cut(s)	331, 413
AsuC2I	CCSGG	3 cut(s)	199, 411, 978
AsuHPI	GGTGA	3 cut(s)	38, 197, 539
AvaI	CYCGRG	2 cut(s)	501, 603
AvaII	GGWCC	1 cut(s)	331
BaeGI	GKGCMC	1 cut(s)	684
BamHI	GGATCC	2 cut(s)	452, 1020
BanI	GGYRCC	1 cut(s)	318
BarI	GAAGNNNNNNTAC	4 cut(s)	338, 370, 380, 412
Bbv12I	GWGCWC	1 cut(s)	684
BbvI	GCAGC	4 cut(s)	361, 572, 875, 1023
BccI	CCATC	4 cut(s)	79, 603, 1006, 1125
BceAI	ACGGC	1 cut(s)	191
BciT130I	CCWGG	1 cut(s)	1111
BclI	TGATCA	3 cut(s)	596, 827, 1004
BcnI	CCSGG	3 cut(s)	199, 411, 978
BcoDI	GTCTC	1 cut(s)	498
BfmI	CTRYAG	2 cut(s)	445, 1075
BfoI	RGCGCY	1 cut(s)	322
BisI	GCNGC	8 cut(s)	350, 488, 561, 797, 800, 844, 864, 1012
BlsI	GCNGC	8 cut(s)	351, 489, 562, 798, 801, 845, 865, 1013
Bme1390I	CCNGG	4 cut(s)	199, 411, 978, 1111
Bme18I	GGWCC	1 cut(s)	331
BmeRI	GACNNNNNGTC	1 cut(s)	329
BmeT110I	CYCGRG	2 cut(s)	501, 603
BmgT120I	GGNCC	2 cut(s)	331, 413
BmiI	GGNNCC	6 cut(s)	320, 415, 454, 476, 482, 1022
BmrFI	CCNGG	4 cut(s)	199, 411, 978, 1111
BmrI	ACTGGG	2 cut(s)	1019, 1053
BmuI	ACTGGG	2 cut(s)	1019, 1053
BpmI	CTGGAG	1 cut(s)	856
BpuMI	CCSGG	3 cut(s)	199, 411, 978
BsaHI	GRCGYC	3 cut(s)	86, 145, 319
BsaJI	CCNNGG	4 cut(s)	169, 418, 663, 794
BsaXI	ACNNNNNCTCC	2 cut(s)	482, 512
Bsc4I	CCNNNNNNNGG	4 cut(s)	328, 423, 798, 1131
Bse1I	ACTGG	6 cut(s)	476, 854, 873, 1025, 1059, 1068
BseBI	CCWGG	1 cut(s)	1111
BseDI	CCNNGG	4 cut(s)	169, 418, 663, 794
BseGI	GGATG	1 cut(s)	614
BseLI	CCNNNNNNNGG	4 cut(s)	328, 423, 798, 1131
BseNI	ACTGG	6 cut(s)	476, 854, 873, 1025, 1059, 1068
BseSI	GKGCMC	1 cut(s)	684
BseXI	GCAGC	4 cut(s)	361, 572, 875, 1023
BseYI	CCCAGC	2 cut(s)	229, 1014
Bsh1236I	CGCG	4 cut(s)	32, 193, 369, 796
Bsh1285I	CGRYCG	1 cut(s)	260
BshFI	GGCC	1 cut(s)	414
BshNI	GGYRCC	1 cut(s)	318
BsiEI	CGRYCG	1 cut(s)	260
BsiHKAI	GWGCWC	1 cut(s)	684
BsiHKCI	CYCGRG	2 cut(s)	501, 603
BsiSI	CCGG	7 cut(s)	198, 411, 459, 478, 484, 557, 977
BslFI	GGGAC	3 cut(s)	214, 246, 1110
BslI	CCNNNNNNNGG	4 cut(s)	328, 423, 798, 1131
BsmAI	GTCTC	1 cut(s)	498
BsmFI	GGGAC	3 cut(s)	214, 246, 1110
BsmI	GAATGC	1 cut(s)	1120
BsnI	GGCC	1 cut(s)	414
BsoBI	CYCGRG	2 cut(s)	501, 603
Bsp1286I	GDGCHC	1 cut(s)	684
Bsp19I	CCATGG	1 cut(s)	169
BspANI	GGCC	1 cut(s)	414
BspFNI	CGCG	4 cut(s)	32, 193, 369, 796
BspHI	TCATGA	3 cut(s)	824, 1001, 1046
BspLI	GGNNCC	6 cut(s)	320, 415, 454, 476, 482, 1022
BspPI	GGATC	8 cut(s)	148, 189, 434, 447, 460, 726, 1015, 1028
BspT107I	GGYRCC	1 cut(s)	318
BsrI	ACTGG	6 cut(s)	476, 854, 873, 1025, 1059, 1068
BssECI	CCNNGG	4 cut(s)	169, 418, 663, 794
BssNI	GRCGYC	3 cut(s)	86, 145, 319
BssT1I	CCWWGG	2 cut(s)	169, 663
Bst2UI	CCWGG	1 cut(s)	1111
Bst4CI	ACNGT	9 cut(s)	237, 272, 376, 618, 701, 743, 771, 782, 947
BstACI	GRCGYC	3 cut(s)	86, 145, 319
BstAPI	GCANNNNNTGC	1 cut(s)	887
BstC8I	GCNNGC	2 cut(s)	111, 983
BstDEI	CTNAG	2 cut(s)	276, 583
BstDSI	CCRYGG	3 cut(s)	169, 418, 794
BstF5I	GGATG	1 cut(s)	614
BstFNI	CGCG	4 cut(s)	32, 193, 369, 796
BstH2I	RGCGCY	1 cut(s)	322
BstHHI	GCGC	2 cut(s)	321, 881
BstMAI	GTCTC	1 cut(s)	498
BstMCI	CGRYCG	1 cut(s)	260
BstMWI	GCNNNNNNNGC	5 cut(s)	115, 487, 557, 688, 887
BstNI	CCWGG	1 cut(s)	1111
BstSCI	CCNGG	4 cut(s)	197, 409, 976, 1109
BstSFI	CTRYAG	2 cut(s)	445, 1075
BstSLI	GKGCMC	1 cut(s)	684
BstUI	CGCG	4 cut(s)	32, 193, 369, 796
BstV1I	GCAGC	4 cut(s)	361, 572, 875, 1023
BstX2I	RGATCY	4 cut(s)	426, 452, 731, 1020
BstYI	RGATCY	4 cut(s)	426, 452, 731, 1020
BsuRI	GGCC	1 cut(s)	414
BtgI	CCRYGG	3 cut(s)	169, 418, 794
BtsCI	GGATG	1 cut(s)	614
BtsIMutI	CAGTG	3 cut(s)	697, 767, 1032
Cac8I	GCNNGC	2 cut(s)	111, 983
CciI	TCATGA	3 cut(s)	824, 1001, 1046
CfoI	GCGC	2 cut(s)	321, 881
Cfr13I	GGNCC	2 cut(s)	331, 413
Cfr42I	CCGCGG	1 cut(s)	797
CseI	GACGC	2 cut(s)	21, 94
Csp6I	GTAC	4 cut(s)	273, 988, 1065, 1104
CviAII	CATG	7 cut(s)	42, 106, 170, 825, 887, 1002, 1047
CviQI	GTAC	4 cut(s)	273, 988, 1065, 1104
DdeI	CTNAG	2 cut(s)	276, 583
DinI	GGCGCC	1 cut(s)	320
DriI	GACNNNNNGTC	1 cut(s)	329
Eam1105I	GACNNNNNGTC	1 cut(s)	329
EciI	GGCGGA	3 cut(s)	152, 445, 638
Eco130I	CCWWGG	2 cut(s)	169, 663
Eco47I	GGWCC	1 cut(s)	331
Eco57I	CTGAAG	2 cut(s)	233, 748
Eco88I	CYCGRG	2 cut(s)	501, 603
EcoRI	GAATTC	1 cut(s)	282
EcoRII	CCWGG	1 cut(s)	1109
EcoT14I	CCWWGG	2 cut(s)	169, 663
EcoT22I	ATGCAT	1 cut(s)	892
EgeI	GGCGCC	1 cut(s)	320
EheI	GGCGCC	1 cut(s)	320
ErhI	CCWWGG	2 cut(s)	169, 663
FaeI	CATG	7 cut(s)	45, 109, 173, 828, 890, 1005, 1050
FalI	AAGNNNNNCTT	2 cut(s)	1075, 1107
FaqI	GGGAC	3 cut(s)	214, 246, 1110
FatI	CATG	7 cut(s)	41, 105, 169, 824, 886, 1001, 1046
FauI	CCCGC	3 cut(s)	335, 795, 801
FbaI	TGATCA	3 cut(s)	596, 827, 1004
FblI	GTMKAC	1 cut(s)	468
Fnu4HI	GCNGC	8 cut(s)	350, 488, 561, 797, 800, 844, 864, 1012
FokI	GGATG	1 cut(s)	621
Fsp4HI	GCNGC	8 cut(s)	350, 488, 561, 797, 800, 844, 864, 1012
FspI	TGCGCA	1 cut(s)	880
GlaI	GCGC	2 cut(s)	320, 880
GluI	GCNGC	8 cut(s)	350, 488, 561, 797, 800, 844, 864, 1012
GsaI	CCCAGC	2 cut(s)	233, 1018
GsuI	CTGGAG	1 cut(s)	856
HaeII	RGCGCY	1 cut(s)	322
HaeIII	GGCC	1 cut(s)	414
HapII	CCGG	7 cut(s)	198, 411, 459, 478, 484, 557, 977
HgaI	GACGC	2 cut(s)	21, 94
HhaI	GCGC	2 cut(s)	321, 881
Hin1I	GRCGYC	3 cut(s)	86, 145, 319
Hin1II	CATG	7 cut(s)	45, 109, 173, 828, 890, 1005, 1050
Hin6I	GCGC	2 cut(s)	319, 879
HinP1I	GCGC	2 cut(s)	319, 879
HinfI	GANTC	2 cut(s)	202, 553
HpaII	CCGG	7 cut(s)	198, 411, 459, 478, 484, 557, 977
HphI	GGTGA	3 cut(s)	38, 197, 539
Hpy166II	GTNNAC	2 cut(s)	469, 682
Hpy188I	TCNGA	5 cut(s)	153, 335, 407, 426, 728
Hpy188III	TCNNGA	7 cut(s)	261, 381, 501, 603, 825, 1002, 1047
Hpy8I	GTNNAC	2 cut(s)	469, 682
Hpy99I	CGWCG	7 cut(s)	88, 147, 150, 309, 327, 743, 755
HpyAV	CCTTC	7 cut(s)	25, 103, 518, 533, 632, 753, 1077
HpyCH4III	ACNGT	9 cut(s)	237, 272, 376, 618, 701, 743, 771, 782, 947
HpyCH4IV	ACGT	3 cut(s)	145, 719, 753
HpyCH4V	TGCA	6 cut(s)	682, 697, 786, 863, 890, 1030
HpyF10VI	GCNNNNNNNGC	5 cut(s)	115, 487, 557, 688, 887
HpyF3I	CTNAG	2 cut(s)	276, 583
HpySE526I	ACGT	3 cut(s)	145, 719, 753
Hsp92I	GRCGYC	3 cut(s)	86, 145, 319
Hsp92II	CATG	7 cut(s)	45, 109, 173, 828, 890, 1005, 1050
HspAI	GCGC	2 cut(s)	319, 879
KasI	GGCGCC	1 cut(s)	318
Ksp22I	TGATCA	3 cut(s)	596, 827, 1004
KspI	CCGCGG	1 cut(s)	797
LmnI	GCTCC	3 cut(s)	480, 486, 667
Lsp1109I	GCAGC	4 cut(s)	361, 572, 875, 1023
MaeII	ACGT	3 cut(s)	145, 719, 753
MaeIII	GTNAC	2 cut(s)	44, 261
MboII	GAAGA	4 cut(s)	88, 239, 716, 741
MflI	RGATCY	4 cut(s)	426, 452, 731, 1020
MhlI	GDGCHC	1 cut(s)	684
MluCI	AATT	9 cut(s)	58, 65, 101, 282, 293, 745, 904, 922, 938
MluI	ACGCGT	1 cut(s)	30
Mly113I	GGCGCC	1 cut(s)	319
MlyI	GAGTC	1 cut(s)	196
MmeI	TCCRAC	5 cut(s)	404, 430, 759, 921, 954
Mph1103I	ATGCAT	1 cut(s)	892
MroXI	GAANNNNTTC	2 cut(s)	376, 1087
MseI	TTAA	3 cut(s)	646, 858, 1036
MspA1I	CMGCKG	2 cut(s)	796, 1014
MspI	CCGG	7 cut(s)	198, 411, 459, 478, 484, 557, 977
MspR9I	CCNGG	4 cut(s)	199, 411, 978, 1111
Mva1269I	GAATGC	1 cut(s)	1120
MvaI	CCWGG	1 cut(s)	1111
MvnI	CGCG	4 cut(s)	32, 193, 369, 796
MwoI	GCNNNNNNNGC	5 cut(s)	115, 487, 557, 688, 887
NarI	GGCGCC	1 cut(s)	319
NciI	CCSGG	3 cut(s)	199, 411, 978
NcoI	CCATGG	1 cut(s)	169
NlaIII	CATG	7 cut(s)	45, 109, 173, 828, 890, 1005, 1050
NlaIV	GGNNCC	6 cut(s)	320, 415, 454, 476, 482, 1022
NmeAIII	GCCGAG	1 cut(s)	250
NmuCI	GTSAC	2 cut(s)	44, 261
NsbI	TGCGCA	1 cut(s)	880
NsiI	ATGCAT	1 cut(s)	892
PaeR7I	CTCGAG	1 cut(s)	603
PagI	TCATGA	3 cut(s)	824, 1001, 1046
PcsI	WCGNNNNNNNCGW	3 cut(s)	322, 331, 747
PctI	GAATGC	1 cut(s)	1120
PdmI	GAANNNNTTC	2 cut(s)	376, 1087
PfeI	GAWTC	1 cut(s)	553
PflFI	GACNNNGTC	1 cut(s)	147
PfoI	TCCNGGA	2 cut(s)	197, 1109
PkrI	GCNGC	8 cut(s)	351, 489, 562, 798, 801, 845, 865, 1013
Ple19I	CGATCG	1 cut(s)	260
PleI	GAGTC	1 cut(s)	196
PluTI	GGCGCC	1 cut(s)	322
PpsI	GAGTC	1 cut(s)	196
PshBI	ATTAAT	1 cut(s)	1036
Psp1406I	AACGTT	1 cut(s)	719
Psp6I	CCWGG	1 cut(s)	1109
PspFI	CCCAGC	2 cut(s)	229, 1014
PspGI	CCWGG	1 cut(s)	1109
PspN4I	GGNNCC	6 cut(s)	320, 415, 454, 476, 482, 1022
PspPI	GGNCC	2 cut(s)	331, 413
PsrI	GAACNNNNNNTAC	2 cut(s)	1054, 1086
PsuI	RGATCY	4 cut(s)	426, 452, 731, 1020
PsyI	GACNNNGTC	1 cut(s)	147
PvuI	CGATCG	1 cut(s)	260
PvuII	CAGCTG	1 cut(s)	1014
RsaI	GTAC	4 cut(s)	274, 989, 1066, 1105
RsaNI	GTAC	4 cut(s)	273, 988, 1065, 1104
SacII	CCGCGG	1 cut(s)	797
SaqAI	TTAA	3 cut(s)	646, 858, 1036
SatI	GCNGC	8 cut(s)	350, 488, 561, 797, 800, 844, 864, 1012
Sau96I	GGNCC	2 cut(s)	331, 413
SchI	GAGTC	1 cut(s)	196
ScrFI	CCNGG	4 cut(s)	199, 411, 978, 1111
SduI	GDGCHC	1 cut(s)	684
SfcI	CTRYAG	2 cut(s)	445, 1075
SfoI	GGCGCC	1 cut(s)	320
Sfr274I	CTCGAG	1 cut(s)	603
Sfr303I	CCGCGG	1 cut(s)	797
SgrBI	CCGCGG	1 cut(s)	797
SinI	GGWCC	1 cut(s)	331
SlaI	CTCGAG	1 cut(s)	603
SmlI	CTYRAG	1 cut(s)	603
SmoI	CTYRAG	1 cut(s)	603
Sse9I	AATT	9 cut(s)	58, 65, 101, 282, 293, 745, 904, 922, 938
SspDI	GGCGCC	1 cut(s)	318
SspI	AATATT	1 cut(s)	815
StyD4I	CCNGG	4 cut(s)	197, 409, 976, 1109
StyI	CCWWGG	2 cut(s)	169, 663
TaaI	ACNGT	9 cut(s)	237, 272, 376, 618, 701, 743, 771, 782, 947
TaiI	ACGT	3 cut(s)	148, 722, 756
TaqI	TCGA	2 cut(s)	604, 1129
TasI	AATT	9 cut(s)	58, 65, 101, 282, 293, 745, 904, 922, 938
TauI	GCSGC	4 cut(s)	490, 799, 802, 846
TfiI	GAWTC	1 cut(s)	553
Tru1I	TTAA	3 cut(s)	646, 858, 1036
Tru9I	TTAA	3 cut(s)	646, 858, 1036
TscAI	CASTG	3 cut(s)	704, 774, 1032
TseFI	GTSAC	2 cut(s)	44, 261
TseI	GCWGC	4 cut(s)	349, 560, 863, 1011
Tsp45I	GTSAC	2 cut(s)	44, 261
TspDTI	ATGAA	4 cut(s)	94, 772, 875, 1096
TspGWI	ACGGA	1 cut(s)	1114
TspRI	CASTG	3 cut(s)	704, 774, 1032
Tth111I	GACNNNGTC	1 cut(s)	147
VneI	GTGCAC	1 cut(s)	680
VpaK11BI	GGWCC	1 cut(s)	331
VspI	ATTAAT	1 cut(s)	1036
XapI	RAATTY	2 cut(s)	282, 293
XhoI	CTCGAG	1 cut(s)	603
XmiI	GTMKAC	1 cut(s)	468
XmnI	GAANNNNTTC	2 cut(s)	376, 1087
ZraI	GACGTC	1 cut(s)	146
Zsp2I	ATGCAT	1 cut(s)	892

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.