MD16G1063200.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0006868
Physical Location & Seq
Reverse (-)
4485285 .. 4490649
5365 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1063200.v1.1.491

Sequence Viewer

Length: 975 bp
ATGGACGAAAACAACAAAAAACGCGATCCACTAACCACGACCGAGTTCACGACTCAGTCCACCTGGACTCCGGACTCCGACAGCGTCTACTTCTTCAGTACCACCCACGACAGAGACAGCAACATACTCAGCGACTTCGGCTGGAACTTCAACCCGGTCGCCTCCAAACCCGATGAAGTCTTCCCCTGCCTCGACCCCATCGCCGCCGCCGACGACATGTCCGATTTGGCGGGAAATACTAGCAGTAGTCCTCTTGTTTTGGAGAACAGCAGCAGCAGTAGCAGCCCCGCCTTTCGGAGCTCCGACCTGGCCCCGGTTGTCGGATCCGCTTCGACCAATCAGTCCGCGTCTACGAGCTCCAGCGAGGATCCTCCCGAGAGGTCTGCAGGCTCCGGCGGCAAACCACCGCCCGAGATACAGAGTAAGGTTAAAAAGAAGGGGCCGAAGAGAATCCGGCAGCAACGATTCGCATTCGTGACAAAGAGTGAAGTTGATCATCTGGAGGATGGCTACCGATGGAGGAAATATGGTCAGAAGGCTGTTAAAAATAGTCCATTTCCAAGGAGCTATTATCGCTGCACAAATACCAAATGCACAGTGAAGAAACGAGTTGAGCGTTCCTCTGAAGATCCCACAACTGTAATTACCACATATGAAGGCCAACACTGTCATCATACCGTTGCATTCCCTCGTGGGGGAGTCATTGGTCATGAATCTGGCTTTGCAGGTCACCAGTTGGCTCAGCTGTCTCCAGCCTCACACTTCATGTATTATCCAACAATTCGACCCAGAGAAATAACTAGTCCTATTAATTTGACCCCAACGGCGTCGCACCAAGCAGTAGTCCGACGTCATGACGATGATCAAGCTGCAGGAGGATCATCCCACTTCATTGATCCTCAGCCAACCCCACCTCTTCCTACCGATGAAGGGCTTCTTGGAGACATTGTACCTCCCGGAATGCGAAATCGATAA

Protein Analysis

325

Amino Acids

35.54

Weight (kDa)

6.6

Isoelectric Point (pI)

61.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 168 - 224 4.4e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 340
AatII GACGTC 1 cut(s) 853
Acc36I ACCTGC 1 cut(s) 716
AccI GTMKAC 2 cut(s) 87, 350
AccII CGCG 2 cut(s) 24, 347
AccIII TCCGGA 1 cut(s) 70
AciI CCGC 8 cut(s) 204, 207, 230, 288, 327, 345, 396, 407
AclWI GGATC 8 cut(s) 20, 318, 331, 362, 375, 623, 886, 890
AcuI CTGAAG 2 cut(s) 79, 645
AcyI GRCGYC 2 cut(s) 827, 850
AfaI GTAC 2 cut(s) 100, 951
AfiI CCNNNNNNNGG 6 cut(s) 294, 313, 320, 694, 695, 930
AflIII ACRYGT 1 cut(s) 216
AgsI TTSAA 1 cut(s) 151
AhdI GACNNNNNGTC 1 cut(s) 217
AhlI ACTAGT 1 cut(s) 800
AjnI CCWGG 2 cut(s) 62, 306
AjuI GAANNNNNNNTTGG 2 cut(s) 921, 953
AluBI AGCT 5 cut(s) 300, 357, 567, 745, 869
AluI AGCT 5 cut(s) 300, 357, 567, 745, 869
Alw21I GWGCWC 2 cut(s) 302, 359
Alw26I GTCTC 3 cut(s) 108, 753, 936
AlwI GGATC 8 cut(s) 20, 318, 331, 362, 375, 623, 886, 890
Ama87I CYCGRG 2 cut(s) 374, 410
Aor13HI TCCGGA 1 cut(s) 70
AoxI GGCC 3 cut(s) 309, 440, 658
ApeKI GCWGC 6 cut(s) 270, 273, 282, 457, 576, 869
AseI ATTAAT 1 cut(s) 810
Asp700I GAANNNNTTC 1 cut(s) 933
AspS9I GGNCC 2 cut(s) 310, 440
AsuC2I CCSGG 3 cut(s) 155, 314, 957
AsuHPI GGTGA 1 cut(s) 722
AvaI CYCGRG 2 cut(s) 374, 410
BamHI GGATCC 2 cut(s) 323, 367
BanII GRGCYC 2 cut(s) 302, 359
BauI CACGAG 1 cut(s) 690
BbsI GAAGAC 1 cut(s) 172
Bbv12I GWGCWC 2 cut(s) 302, 359
BbvCI CCTCAGC 1 cut(s) 900
BbvI GCAGC 6 cut(s) 282, 285, 294, 469, 563, 856
BccI CCATC 3 cut(s) 206, 500, 510
BceAI ACGGC 1 cut(s) 840
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 2 cut(s) 64, 308
BclI TGATCA 2 cut(s) 493, 862
BcnI CCSGG 3 cut(s) 155, 314, 957
BcoDI GTCTC 3 cut(s) 108, 753, 936
BcuI ACTAGT 1 cut(s) 800
BfaI CTAG 2 cut(s) 240, 801
BfmI CTRYAG 2 cut(s) 384, 870
BfuAI ACCTGC 1 cut(s) 716
BisI GCNGC 9 cut(s) 204, 207, 271, 274, 283, 397, 458, 577, 870
BlpI GCTNAGC 1 cut(s) 741
BlsI GCNGC 9 cut(s) 205, 208, 272, 275, 284, 398, 459, 578, 871
Bme1390I CCNGG 5 cut(s) 64, 155, 308, 314, 957
BmeRI GACNNNNNGTC 1 cut(s) 217
BmeT110I CYCGRG 2 cut(s) 374, 410
BmgT120I GGNCC 2 cut(s) 310, 440
BmiI GGNNCC 5 cut(s) 312, 325, 369, 391, 441
BmrFI CCNGG 5 cut(s) 64, 155, 308, 314, 957
BpiI GAAGAC 1 cut(s) 172
BplI GAGNNNNNCTC 2 cut(s) 605, 637
BpmI CTGGAG 3 cut(s) 343, 521, 735
Bpu10I CCTNAGC 1 cut(s) 900
Bpu1102I GCTNAGC 1 cut(s) 741
BpuMI CCSGG 3 cut(s) 155, 314, 957
Bsa29I ATCGAT 1 cut(s) 970
BsaHI GRCGYC 2 cut(s) 827, 850
BsaJI CCNNGG 2 cut(s) 312, 560
BsaWI WCCGGW 1 cut(s) 70
BsaXI ACNNNNNCTCC 4 cut(s) 52, 82, 933, 963
Bsc4I CCNNNNNNNGG 6 cut(s) 294, 313, 320, 694, 695, 930
Bse1I ACTGG 1 cut(s) 733
BseAI TCCGGA 1 cut(s) 70
BseBI CCWGG 2 cut(s) 64, 308
BseCI ATCGAT 1 cut(s) 970
BseDI CCNNGG 2 cut(s) 312, 560
BseGI GGATG 2 cut(s) 511, 881
BseLI CCNNNNNNNGG 6 cut(s) 294, 313, 320, 694, 695, 930
BseMII CTCAG 4 cut(s) 68, 142, 755, 914
BseNI ACTGG 1 cut(s) 733
BseXI GCAGC 6 cut(s) 282, 285, 294, 469, 563, 856
BsgI GTGCAG 1 cut(s) 562
Bsh1236I CGCG 2 cut(s) 24, 347
Bsh1285I CGRYCG 2 cut(s) 42, 159
BshFI GGCC 3 cut(s) 311, 442, 660
BshVI ATCGAT 1 cut(s) 970
BsiEI CGRYCG 2 cut(s) 42, 159
BsiHKAI GWGCWC 2 cut(s) 302, 359
BsiHKCI CYCGRG 2 cut(s) 374, 410
BsiSI CCGG 6 cut(s) 71, 155, 314, 393, 454, 957
BslI CCNNNNNNNGG 6 cut(s) 294, 313, 320, 694, 695, 930
BsmAI GTCTC 3 cut(s) 108, 753, 936
BsmI GAATGC 3 cut(s) 470, 683, 966
BsnI GGCC 3 cut(s) 311, 442, 660
BsoBI CYCGRG 2 cut(s) 374, 410
Bsp1286I GDGCHC 2 cut(s) 302, 359
Bsp13I TCCGGA 1 cut(s) 70
Bsp143I GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
Bsp1720I GCTNAGC 1 cut(s) 741
BspACI CCGC 8 cut(s) 204, 207, 230, 288, 327, 345, 396, 407
BspANI GGCC 3 cut(s) 311, 442, 660
BspCNI CTCAG 4 cut(s) 67, 141, 754, 913
BspDI ATCGAT 1 cut(s) 970
BspEI TCCGGA 1 cut(s) 70
BspFNI CGCG 2 cut(s) 24, 347
BspHI TCATGA 2 cut(s) 709, 853
BspLI GGNNCC 5 cut(s) 312, 325, 369, 391, 441
BspMAI CTGCAG 2 cut(s) 388, 874
BspMI ACCTGC 1 cut(s) 716
BspPI GGATC 8 cut(s) 20, 318, 331, 362, 375, 623, 886, 890
BsrI ACTGG 1 cut(s) 733
BssECI CCNNGG 2 cut(s) 312, 560
BssMI GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
BssNI GRCGYC 2 cut(s) 827, 850
BssSI CACGAG 1 cut(s) 690
BssT1I CCWWGG 1 cut(s) 560
Bst2BI CACGAG 1 cut(s) 690
Bst2UI CCWGG 2 cut(s) 64, 308
Bst4CI ACNGT 4 cut(s) 598, 640, 668, 679
Bst6I CTCTTC 2 cut(s) 440, 921
BstACI GRCGYC 2 cut(s) 827, 850
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 4 cut(s) 54, 128, 741, 900
BstEII GGTNACC 1 cut(s) 728
BstF5I GGATG 2 cut(s) 511, 881
BstFNI CGCG 2 cut(s) 24, 347
BstKTI GATC 8 cut(s) 28, 326, 370, 496, 631, 865, 881, 898
BstMAI GTCTC 3 cut(s) 108, 753, 936
BstMBI GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
BstMCI CGRYCG 2 cut(s) 42, 159
BstMWI GCNNNNNNNGC 5 cut(s) 138, 279, 282, 396, 573
BstNI CCWGG 2 cut(s) 64, 308
BstNSI RCATGY 1 cut(s) 220
BstPI GGTNACC 1 cut(s) 728
BstSCI CCNGG 5 cut(s) 62, 153, 306, 312, 955
BstSFI CTRYAG 2 cut(s) 384, 870
BstUI CGCG 2 cut(s) 24, 347
BstV1I GCAGC 6 cut(s) 282, 285, 294, 469, 563, 856
BstV2I GAAGAC 1 cut(s) 172
BstX2I RGATCY 3 cut(s) 323, 367, 628
BstYI RGATCY 3 cut(s) 323, 367, 628
Bsu15I ATCGAT 1 cut(s) 970
BsuRI GGCC 3 cut(s) 311, 442, 660
BsuTUI ATCGAT 1 cut(s) 970
BtgZI GCGATG 1 cut(s) 184
BtsCI GGATG 2 cut(s) 511, 881
BtsIMutI CAGTG 2 cut(s) 603, 664
BveI ACCTGC 1 cut(s) 716
Cac8I GCNNGC 1 cut(s) 388
CciI TCATGA 2 cut(s) 709, 853
Cfr13I GGNCC 2 cut(s) 310, 440
ClaI ATCGAT 1 cut(s) 970
CseI GACGC 3 cut(s) 73, 336, 816
Csp6I GTAC 2 cut(s) 99, 950
CviAII CATG 4 cut(s) 217, 710, 766, 854
CviQI GTAC 2 cut(s) 99, 950
DdeI CTNAG 4 cut(s) 54, 128, 741, 900
DpnI GATC 8 cut(s) 27, 325, 369, 495, 630, 864, 880, 897
DpnII GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
DrdI GACNNNNNNGTC 1 cut(s) 340
DriI GACNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 440, 921
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 2 cut(s) 440, 921
Ecl136II GAGCTC 2 cut(s) 300, 357
Eco130I CCWWGG 1 cut(s) 560
Eco24I GRGCYC 2 cut(s) 302, 359
Eco53kI GAGCTC 2 cut(s) 300, 357
Eco57I CTGAAG 2 cut(s) 79, 645
Eco88I CYCGRG 2 cut(s) 374, 410
Eco91I GGTNACC 1 cut(s) 728
EcoICRI GAGCTC 2 cut(s) 300, 357
EcoO65I GGTNACC 1 cut(s) 728
EcoRII CCWGG 2 cut(s) 62, 306
EcoT14I CCWWGG 1 cut(s) 560
EcoT38I GRGCYC 2 cut(s) 302, 359
ErhI CCWWGG 1 cut(s) 560
FaeI CATG 4 cut(s) 220, 713, 769, 857
FaiI YATR 9 cut(s) 125, 218, 528, 652, 654, 675, 711, 767, 855
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FatI CATG 4 cut(s) 216, 709, 765, 853
FauI CCCGC 2 cut(s) 223, 295
FauNDI CATATG 1 cut(s) 652
FbaI TGATCA 2 cut(s) 493, 862
FblI GTMKAC 2 cut(s) 87, 350
Fnu4HI GCNGC 9 cut(s) 204, 207, 271, 274, 283, 397, 458, 577, 870
FokI GGATG 2 cut(s) 518, 868
FriOI GRGCYC 2 cut(s) 302, 359
Fsp4HI GCNGC 9 cut(s) 204, 207, 271, 274, 283, 397, 458, 577, 870
FspBI CTAG 2 cut(s) 240, 801
GluI GCNGC 9 cut(s) 204, 207, 271, 274, 283, 397, 458, 577, 870
GsuI CTGGAG 3 cut(s) 343, 521, 735
HaeIII GGCC 3 cut(s) 311, 442, 660
HapII CCGG 6 cut(s) 71, 155, 314, 393, 454, 957
HgaI GACGC 3 cut(s) 73, 336, 816
Hin1I GRCGYC 2 cut(s) 827, 850
Hin1II CATG 4 cut(s) 220, 713, 769, 857
HinfI GANTC 7 cut(s) 52, 67, 74, 450, 465, 699, 713
HpaII CCGG 6 cut(s) 71, 155, 314, 393, 454, 957
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 4 cut(s) 48, 60, 88, 351
Hpy188I TCNGA 8 cut(s) 79, 223, 297, 304, 323, 534, 625, 848
Hpy188III TCNNGA 7 cut(s) 49, 71, 374, 475, 500, 710, 854
Hpy8I GTNNAC 4 cut(s) 48, 60, 88, 351
Hpy99I CGWCG 3 cut(s) 215, 832, 852
HpyAV CCTTC 4 cut(s) 430, 529, 650, 923
HpyCH4III ACNGT 4 cut(s) 598, 640, 668, 679
HpyCH4IV ACGT 1 cut(s) 850
HpyCH4V TGCA 6 cut(s) 386, 579, 594, 683, 725, 872
HpyF10VI GCNNNNNNNGC 5 cut(s) 138, 279, 282, 396, 573
HpyF3I CTNAG 4 cut(s) 54, 128, 741, 900
HpySE526I ACGT 1 cut(s) 850
Hsp92I GRCGYC 2 cut(s) 827, 850
Hsp92II CATG 4 cut(s) 220, 713, 769, 857
Kpn2I TCCGGA 1 cut(s) 70
Ksp22I TGATCA 2 cut(s) 493, 862
Kzo9I GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
LmnI GCTCC 5 cut(s) 297, 305, 362, 395, 564
Lsp1109I GCAGC 6 cut(s) 282, 285, 294, 469, 563, 856
MaeI CTAG 2 cut(s) 240, 801
MaeII ACGT 1 cut(s) 850
MaeIII GTNAC 2 cut(s) 475, 728
MalI GATC 8 cut(s) 27, 325, 369, 495, 630, 864, 880, 897
MboI GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
MboII GAAGA 6 cut(s) 85, 172, 457, 613, 638, 908
MflI RGATCY 3 cut(s) 323, 367, 628
MhlI GDGCHC 2 cut(s) 302, 359
MluCI AATT 3 cut(s) 642, 780, 811
MlyI GAGTC 4 cut(s) 46, 61, 68, 708
MmeI TCCRAC 5 cut(s) 102, 301, 327, 800, 871
MroI TCCGGA 1 cut(s) 70
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 3 cut(s) 429, 543, 810
MslI CAYNNNNRTG 1 cut(s) 858
MspA1I CMGCKG 1 cut(s) 745
MspI CCGG 6 cut(s) 71, 155, 314, 393, 454, 957
MspR9I CCNGG 5 cut(s) 64, 155, 308, 314, 957
Mva1269I GAATGC 3 cut(s) 470, 683, 966
MvaI CCWGG 2 cut(s) 64, 308
MvnI CGCG 2 cut(s) 24, 347
MwoI GCNNNNNNNGC 5 cut(s) 138, 279, 282, 396, 573
NciI CCSGG 3 cut(s) 155, 314, 957
NdeI CATATG 1 cut(s) 652
NdeII GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
NlaIII CATG 4 cut(s) 220, 713, 769, 857
NlaIV GGNNCC 5 cut(s) 312, 325, 369, 391, 441
NmuCI GTSAC 2 cut(s) 475, 728
NspI RCATGY 1 cut(s) 220
PagI TCATGA 2 cut(s) 709, 853
PciI ACATGT 1 cut(s) 216
PcsI WCGNNNNNNNCGW 3 cut(s) 207, 219, 613
PctI GAATGC 3 cut(s) 470, 683, 966
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 3 cut(s) 450, 465, 713
PflFI GACNNNGTC 2 cut(s) 55, 83
PfoI TCCNGGA 1 cut(s) 955
PkrI GCNGC 9 cut(s) 205, 208, 272, 275, 284, 398, 459, 578, 871
PleI GAGTC 4 cut(s) 46, 61, 68, 707
PpsI GAGTC 4 cut(s) 46, 61, 68, 707
PscI ACATGT 1 cut(s) 216
PshBI ATTAAT 1 cut(s) 810
Psp124BI GAGCTC 2 cut(s) 302, 359
Psp6I CCWGG 2 cut(s) 62, 306
PspEI GGTNACC 1 cut(s) 728
PspGI CCWGG 2 cut(s) 62, 306
PspN4I GGNNCC 5 cut(s) 312, 325, 369, 391, 441
PspPI GGNCC 2 cut(s) 310, 440
PstI CTGCAG 2 cut(s) 388, 874
PsuI RGATCY 3 cut(s) 323, 367, 628
PsyI GACNNNGTC 2 cut(s) 55, 83
PvuII CAGCTG 1 cut(s) 745
RsaI GTAC 2 cut(s) 100, 951
RsaNI GTAC 2 cut(s) 99, 950
RseI CAYNNNNRTG 1 cut(s) 858
SacI GAGCTC 2 cut(s) 302, 359
SaqAI TTAA 3 cut(s) 429, 543, 810
SatI GCNGC 9 cut(s) 204, 207, 271, 274, 283, 397, 458, 577, 870
Sau3AI GATC 8 cut(s) 25, 323, 367, 493, 628, 862, 878, 895
Sau96I GGNCC 2 cut(s) 310, 440
SchI GAGTC 4 cut(s) 46, 61, 68, 708
ScrFI CCNGG 5 cut(s) 64, 155, 308, 314, 957
SduI GDGCHC 2 cut(s) 302, 359
SfcI CTRYAG 2 cut(s) 384, 870
SmiMI CAYNNNNRTG 1 cut(s) 858
SpeI ACTAGT 1 cut(s) 800
Sse9I AATT 3 cut(s) 642, 780, 811
SsiI CCGC 8 cut(s) 204, 207, 230, 288, 327, 345, 396, 407
SspMI CTAG 2 cut(s) 240, 801
SstI GAGCTC 2 cut(s) 302, 359
StyD4I CCNGG 5 cut(s) 62, 153, 306, 312, 955
StyI CCWWGG 1 cut(s) 560
TaaI ACNGT 4 cut(s) 598, 640, 668, 679
TaiI ACGT 1 cut(s) 853
TaqI TCGA 4 cut(s) 192, 332, 784, 970
TaqII GACCGA 1 cut(s) 56
TasI AATT 3 cut(s) 642, 780, 811
TauI GCSGC 3 cut(s) 206, 209, 399
TfiI GAWTC 3 cut(s) 450, 465, 713
Tru1I TTAA 3 cut(s) 429, 543, 810
Tru9I TTAA 3 cut(s) 429, 543, 810
TscAI CASTG 2 cut(s) 603, 671
TseFI GTSAC 2 cut(s) 475, 728
TseI GCWGC 6 cut(s) 270, 273, 282, 457, 576, 869
Tsp45I GTSAC 2 cut(s) 475, 728
TspDTI ATGAA 6 cut(s) 189, 669, 726, 754, 880, 942
TspRI CASTG 2 cut(s) 603, 671
Tth111I GACNNNGTC 2 cut(s) 55, 83
VspI ATTAAT 1 cut(s) 810
XceI RCATGY 1 cut(s) 220
XmiI GTMKAC 2 cut(s) 87, 350
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 2 cut(s) 240, 801
ZraI GACGTC 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.