FvH4_4g31701

finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0018428
Physical Location & Seq
Forward (+)
30741208 .. 30742047
840 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31701.t1

Sequence Viewer

Length: 831 bp
ATGGCATTATTTTGTTGTACTATCGAGACAGTGAATCACGAGGAAACTCACCACAACTATGTCATCAATCCCATTAGAATACCAGCCATAGACCAGCTTCATCATCAAGGGAAAGTTTTCATAGAGTTTGTCTACGAAGTCATCCACAGCTATAAGAATAATTCACTACTCCATCAAAGAACTCTTATCAGCCAATACCAAGATCCTGAGGAGACGAAGATAGAACTGCGCCGATTTGACAGGAGCATGCTAAAGAGCCAGAGACAAACGTCCAGGATTATGTCCAGCGTTCTGCCCATGTTTAGAATCACAAAAGGTACTTCATTGTACAATGAAATAGTCGATGCGATAGTGGAAACGGTTTCAAACAGGGGCAGTTCTAGAGAGACGTCGGCCTTGTCGGTTTGTATTACTAGGCAACATATCATGATTCACTGCGAGATTCACTGCGAGAGGTGCTTGATGAGAGATTATCGTTTGTTGAGATTGAGGGAGGTGGCTTTGATTCAGAGAGCTTTGAGGGAATCGGAAGCCGAGAGAATGCGACAAATGATGGTACCGGCGAGTGAGTCAGCGATCAAGAACCTTTTGAATAGGGTAAGAGTCGAGGAGGAGGAGGAAAAGGAAAGTACAAGAAAAAGAAGAAAATCTAGTTGTAGTAGGAGTGATGAGGTGGGTGAGAGTGGGTATTGCACTGTTTGTTTGGAACCTGTGGGATCATATGCTACTGAGATGCCTTGCAAACATGCGTTTCATGAAGATTGCATAGTGAGATGGCTGAAAGTAAGTCACAAATGTCCAGTCTGCAGATCCGAGGTGCCTACTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

277

Amino Acids

32.24

Weight (kDa)

7.95

Isoelectric Point (pI)

66.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_2 PF13923 230 - 269 7.8e-09 Zinc finger, C3HC4 type (RING finger)
zf-RING_2 PF13639 231 - 270 4.4e-12 Ring finger domain
zf-C3HC4 PF00097 231 - 269 4.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 231 - 256 5.8e-06 RING-like zinc finger
zf-rbx1 PF12678 245 - 270 2.2e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018428)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31701
rosa_chinensis RchiOBHm_Chr4g0440251
rosa_laevigata RLG00000006194
rosa_multiflora Rmu_sc0001685.1_g000033
rosa_roxburghii Rroxscaffold_1G00006460 Rroxscaffold_5G00381170
rosa_samantha Rh4AG376300 Rh4CG403900 Rh4DG383000
rosa_wichuraiana Rw4G032210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 392
Acc65I GGTACC 1 cut(s) 556
AccB1I GGYRCC 2 cut(s) 556, 817
AccI GTMKAC 1 cut(s) 132
AclWI GGATC 3 cut(s) 197, 724, 804
AcyI GRCGYC 1 cut(s) 389
AfaI GTAC 5 cut(s) 19, 319, 329, 558, 631
AgsI TTSAA 2 cut(s) 366, 592
AjnI CCWGG 1 cut(s) 272
AluBI AGCT 3 cut(s) 97, 150, 515
AluI AGCT 3 cut(s) 97, 150, 515
Alw26I GTCTC 4 cut(s) 20, 206, 256, 380
AlwI GGATC 3 cut(s) 197, 724, 804
AoxI GGCC 1 cut(s) 393
ArsI GACNNNNNNTTYG 2 cut(s) 786, 818
Asp700I GAANNNNTTC 1 cut(s) 116
Asp718I GGTACC 1 cut(s) 556
AspLEI GCGC 1 cut(s) 231
AsuHPI GGTGA 2 cut(s) 41, 689
AxyI CCTNAGG 1 cut(s) 207
BanI GGYRCC 2 cut(s) 556, 817
BauI CACGAG 1 cut(s) 38
BccI CCATC 3 cut(s) 180, 547, 768
BciT130I CCWGG 1 cut(s) 274
BcoDI GTCTC 4 cut(s) 20, 206, 256, 380
BfaI CTAG 3 cut(s) 381, 414, 651
BfmI CTRYAG 1 cut(s) 805
Bme1390I CCNGG 1 cut(s) 274
BmiI GGNNCC 3 cut(s) 558, 708, 819
BmrFI CCNGG 1 cut(s) 274
BmsI GCATC 2 cut(s) 334, 723
BoxI GACNNNNGTC 1 cut(s) 268
BsaHI GRCGYC 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 813
Bse118I RCCGGY 1 cut(s) 559
Bse1I ACTGG 1 cut(s) 800
Bse21I CCTNAGG 1 cut(s) 207
BseBI CCWGG 1 cut(s) 274
BseDI CCNNGG 1 cut(s) 813
BseGI GGATG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 198, 720
BseNI ACTGG 1 cut(s) 800
BseRI GAGGAG 4 cut(s) 224, 623, 626, 629
BshFI GGCC 1 cut(s) 395
BshNI GGYRCC 2 cut(s) 556, 817
BsiSI CCGG 1 cut(s) 560
BsmAI GTCTC 4 cut(s) 20, 206, 256, 380
BsmBI CGTCTC 2 cut(s) 206, 380
BsmI GAATGC 1 cut(s) 546
BsnI GGCC 1 cut(s) 395
Bsp1407I TGTACA 1 cut(s) 327
Bsp143I GATC 4 cut(s) 202, 576, 716, 809
BspANI GGCC 1 cut(s) 395
BspCNI CTCAG 2 cut(s) 199, 721
BspHI TCATGA 2 cut(s) 426, 754
BspLI GGNNCC 3 cut(s) 558, 708, 819
BspMAI CTGCAG 1 cut(s) 809
BspPI GGATC 3 cut(s) 197, 724, 804
BspT107I GGYRCC 2 cut(s) 556, 817
BsrFI RCCGGY 1 cut(s) 559
BsrGI TGTACA 1 cut(s) 327
BsrI ACTGG 1 cut(s) 800
BssAI RCCGGY 1 cut(s) 559
BssECI CCNNGG 1 cut(s) 813
BssMI GATC 4 cut(s) 202, 576, 716, 809
BssNI GRCGYC 1 cut(s) 389
BssSI CACGAG 1 cut(s) 38
Bst2BI CACGAG 1 cut(s) 38
Bst2UI CCWGG 1 cut(s) 274
Bst4CI ACNGT 3 cut(s) 31, 361, 697
BstACI GRCGYC 1 cut(s) 389
BstAUI TGTACA 1 cut(s) 327
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 2 cut(s) 207, 729
BstF5I GGATG 1 cut(s) 141
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 4 cut(s) 205, 579, 719, 812
BstMAI GTCTC 4 cut(s) 20, 206, 256, 380
BstMBI GATC 4 cut(s) 202, 576, 716, 809
BstMWI GCNNNNNNNGC 1 cut(s) 456
BstNI CCWGG 1 cut(s) 274
BstNSI RCATGY 2 cut(s) 250, 749
BstPAI GACNNNNGTC 1 cut(s) 268
BstSCI CCNGG 1 cut(s) 272
BstSFI CTRYAG 1 cut(s) 805
BstX2I RGATCY 2 cut(s) 202, 809
BstYI RGATCY 2 cut(s) 202, 809
Bsu36I CCTNAGG 1 cut(s) 207
BsuRI GGCC 1 cut(s) 395
BtsCI GGATG 1 cut(s) 141
BtsI GCAGTG 2 cut(s) 433, 445
BtsIMutI CAGTG 4 cut(s) 36, 433, 445, 693
Cac8I GCNNGC 1 cut(s) 248
CciI TCATGA 2 cut(s) 426, 754
CfoI GCGC 1 cut(s) 231
Cfr10I RCCGGY 1 cut(s) 559
Csp6I GTAC 5 cut(s) 18, 318, 328, 557, 630
CviAII CATG 5 cut(s) 247, 298, 427, 746, 755
CviQI GTAC 5 cut(s) 18, 318, 328, 557, 630
DdeI CTNAG 2 cut(s) 207, 729
DpnI GATC 4 cut(s) 204, 578, 718, 811
DpnII GATC 4 cut(s) 202, 576, 716, 809
Eco81I CCTNAGG 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 272
Esp3I CGTCTC 2 cut(s) 206, 380
FaeI CATG 5 cut(s) 250, 301, 430, 749, 758
FatI CATG 5 cut(s) 246, 297, 426, 745, 754
FauNDI CATATG 1 cut(s) 721
FblI GTMKAC 1 cut(s) 132
FokI GGATG 1 cut(s) 128
FspBI CTAG 3 cut(s) 381, 414, 651
GlaI GCGC 1 cut(s) 230
HaeIII GGCC 1 cut(s) 395
HapII CCGG 1 cut(s) 560
HhaI GCGC 1 cut(s) 231
Hin1I GRCGYC 1 cut(s) 389
Hin1II CATG 5 cut(s) 250, 301, 430, 749, 758
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HinfI GANTC 8 cut(s) 34, 306, 430, 442, 505, 524, 569, 603
HpaII CCGG 1 cut(s) 560
HphI GGTGA 2 cut(s) 41, 689
Hpy166II GTNNAC 1 cut(s) 133
Hpy188I TCNGA 3 cut(s) 510, 529, 814
Hpy188III TCNNGA 7 cut(s) 25, 38, 206, 381, 427, 580, 755
Hpy8I GTNNAC 1 cut(s) 133
Hpy99I CGWCG 1 cut(s) 394
HpyCH4III ACNGT 3 cut(s) 31, 361, 697
HpyCH4IV ACGT 2 cut(s) 269, 389
HpyCH4V TGCA 4 cut(s) 693, 741, 765, 807
HpyF10VI GCNNNNNNNGC 1 cut(s) 456
HpyF3I CTNAG 2 cut(s) 207, 729
HpySE526I ACGT 2 cut(s) 269, 389
Hsp92I GRCGYC 1 cut(s) 389
Hsp92II CATG 5 cut(s) 250, 301, 430, 749, 758
HspAI GCGC 1 cut(s) 229
KpnI GGTACC 1 cut(s) 560
Kzo9I GATC 4 cut(s) 202, 576, 716, 809
LmnI GCTCC 1 cut(s) 243
LweI GCATC 2 cut(s) 334, 723
MaeI CTAG 3 cut(s) 381, 414, 651
MaeII ACGT 2 cut(s) 269, 389
MaeIII GTNAC 1 cut(s) 788
MalI GATC 4 cut(s) 204, 578, 718, 811
MboI GATC 4 cut(s) 202, 576, 716, 809
MboII GAAGA 3 cut(s) 229, 654, 770
MflI RGATCY 2 cut(s) 202, 809
MluCI AATT 1 cut(s) 160
MlyI GAGTC 2 cut(s) 578, 612
MroXI GAANNNNTTC 1 cut(s) 116
MslI CAYNNNNRTG 1 cut(s) 57
MspI CCGG 1 cut(s) 560
MspR9I CCNGG 1 cut(s) 274
Mva1269I GAATGC 1 cut(s) 546
MvaI CCWGG 1 cut(s) 274
MwoI GCNNNNNNNGC 1 cut(s) 456
NdeI CATATG 1 cut(s) 721
NdeII GATC 4 cut(s) 202, 576, 716, 809
NlaIII CATG 5 cut(s) 250, 301, 430, 749, 758
NlaIV GGNNCC 3 cut(s) 558, 708, 819
NmeAIII GCCGAG 1 cut(s) 559
NmuCI GTSAC 1 cut(s) 788
NspI RCATGY 2 cut(s) 250, 749
PaeI GCATGC 1 cut(s) 250
PagI TCATGA 2 cut(s) 426, 754
PctI GAATGC 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 116
PfeI GAWTC 6 cut(s) 34, 306, 430, 442, 505, 524
PfoI TCCNGGA 1 cut(s) 272
PleI GAGTC 2 cut(s) 577, 611
PpsI GAGTC 2 cut(s) 577, 611
PshAI GACNNNNGTC 1 cut(s) 268
Psp6I CCWGG 1 cut(s) 272
PspGI CCWGG 1 cut(s) 272
PspN4I GGNNCC 3 cut(s) 558, 708, 819
PstI CTGCAG 1 cut(s) 809
PsuI RGATCY 2 cut(s) 202, 809
RsaI GTAC 5 cut(s) 19, 319, 329, 558, 631
RsaNI GTAC 5 cut(s) 18, 318, 328, 557, 630
RseI CAYNNNNRTG 1 cut(s) 57
Sau3AI GATC 4 cut(s) 202, 576, 716, 809
SchI GAGTC 2 cut(s) 578, 612
ScrFI CCNGG 1 cut(s) 274
SfaNI GCATC 2 cut(s) 334, 723
SfcI CTRYAG 1 cut(s) 805
SmiMI CAYNNNNRTG 1 cut(s) 57
SphI GCATGC 1 cut(s) 250
Sse9I AATT 1 cut(s) 160
SspMI CTAG 3 cut(s) 381, 414, 651
StyD4I CCNGG 1 cut(s) 272
TaaI ACNGT 3 cut(s) 31, 361, 697
TaiI ACGT 2 cut(s) 272, 392
TaqI TCGA 3 cut(s) 24, 342, 606
TasI AATT 1 cut(s) 160
TatI WGTACW 3 cut(s) 17, 327, 629
TfiI GAWTC 6 cut(s) 34, 306, 430, 442, 505, 524
TscAI CASTG 4 cut(s) 36, 440, 452, 700
TseFI GTSAC 1 cut(s) 788
Tsp45I GTSAC 1 cut(s) 788
TspDTI ATGAA 6 cut(s) 89, 109, 312, 348, 743, 771
TspRI CASTG 4 cut(s) 36, 440, 452, 700
XbaI TCTAGA 1 cut(s) 380
XceI RCATGY 2 cut(s) 250, 749
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 116
XspI CTAG 3 cut(s) 381, 414, 651
ZraI GACGTC 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.