Rw4G032210

finger protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018428
Physical Location & Seq
Forward (+)
59340825 .. 59341619
795 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032210.1

Sequence Viewer

Length: 795 bp
ATGGAGATAGTGAATCACGAGGAAAATCACTTCGATTGTCACATCAATCCCACTAGAATACCAGCACTAGACCAGCTTCATCGTCAAGGGGAACTTTGGATAGAGTTTGTCTACAAAGTCTTCCACGGCTATGAGAATAATTCACTAGACAATCAAAGAACTCGTATCAGGCAATACCAAGATCCTGGGGAGTCGAAGAAAGAACTGCACCGATATGAGATGAGCATGCTAAAGAGCCACAGCCAAACGGCCGGGATTATGTCCGGCGTGCTGCCCATCTTTGGAATCACAAAAGGTACATCTTTGTACAATAAAATAGTGGATGCGATAGTCATTTGGGTTTCAAACAGGGGCAGTTCTCAAGAGTCATTGGCCTTATCGGTACATATTATTAGGCAACATACCAAGATTCACTGCAATCGGTGCTTTCTGAGGGATTTGATATTGGAGGTGGAATTGAGGGAGATGGCTTTGATGGAGAGAGCTCTGAGGGAATCGGAAGCCGAGAGAATGAAGAAAATGATGGTACCAGCGAGTGAGTCAGCGATCAAGAACCTTTTGAAGAGGGTAAGGGTCGAGGAGGAGGAAAAGGAAAGTACAAGAAAAAGAAGAAAATCTAGTAGTAGTAGTAGTGAGGTGGGTGGTGGTGGGTGTTGTACTGTTTGTTTGGAGCCTCTGGGATCATATGCGACTGAGATGCCTTGCAAACATGCGTTTCATGAAGATTGCATAGTGGGATGGCTGAGACTTAGTCACTATTGTCCACTCTGCAGATCCGAGGTGCCAACTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

264

Amino Acids

30.39

Weight (kDa)

7.63

Isoelectric Point (pI)

59.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 218 - 258 2.2e-10 Ring finger domain
zf-rbx1 PF12678 218 - 258 5.3e-08 RING-H2 zinc finger domain
zf-C3HC4 PF00097 219 - 257 7.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 219 - 257 2.7e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 219 - 244 7.1e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018428)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g31701
rosa_chinensis RchiOBHm_Chr4g0440251
rosa_laevigata RLG00000006194
rosa_multiflora Rmu_sc0001685.1_g000033
rosa_roxburghii Rroxscaffold_1G00006460 Rroxscaffold_5G00381170
rosa_samantha Rh4AG376300 Rh4CG403900 Rh4DG383000
rosa_wichuraiana Rw4G032210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 526
AccB1I GGYRCC 2 cut(s) 526, 781
AccI GTMKAC 1 cut(s) 111
AclWI GGATC 3 cut(s) 176, 688, 768
AcoI YGGCCR 1 cut(s) 249
AfaI GTAC 6 cut(s) 298, 308, 384, 528, 598, 658
AfiI CCNNNNNNNGG 1 cut(s) 281
AgsI TTSAA 2 cut(s) 345, 562
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 2 cut(s) 76, 485
AluI AGCT 2 cut(s) 76, 485
Alw21I GWGCWC 1 cut(s) 487
Alw26I GTCTC 1 cut(s) 739
AlwI GGATC 3 cut(s) 176, 688, 768
AoxI GGCC 2 cut(s) 249, 372
ApeKI GCWGC 1 cut(s) 271
Asp718I GGTACC 1 cut(s) 526
AsuC2I CCSGG 1 cut(s) 253
BanI GGYRCC 2 cut(s) 526, 781
BanII GRGCYC 1 cut(s) 487
BauI CACGAG 1 cut(s) 17
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 1 cut(s) 487
BbvI GCAGC 1 cut(s) 258
BccI CCATC 5 cut(s) 284, 460, 469, 517, 732
BceAI ACGGC 2 cut(s) 142, 264
BcgI CGANNNNNNTGC 2 cut(s) 679, 713
BciT130I CCWGG 1 cut(s) 186
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 1 cut(s) 739
BfaI CTAG 4 cut(s) 54, 68, 146, 618
BfmI CTRYAG 1 cut(s) 769
BisI GCNGC 1 cut(s) 272
BlsI GCNGC 1 cut(s) 273
Bme1390I CCNGG 2 cut(s) 186, 253
BmiI GGNNCC 3 cut(s) 528, 672, 783
BmrFI CCNGG 2 cut(s) 186, 253
BmsI GCATC 2 cut(s) 313, 687
BpiI GAAGAC 1 cut(s) 112
BpuEI CTTGAG 1 cut(s) 345
BpuMI CCSGG 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 124, 185, 777
Bsc4I CCNNNNNNNGG 1 cut(s) 281
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 3 cut(s) 124, 185, 777
BseGI GGATG 2 cut(s) 328, 743
BseLI CCNNNNNNNGG 1 cut(s) 281
BseMII CTCAG 4 cut(s) 422, 479, 684, 734
BseRI GAGGAG 2 cut(s) 593, 596
BseX3I CGGCCG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 191
Bsh1285I CGRYCG 1 cut(s) 252
BshFI GGCC 2 cut(s) 251, 374
BshNI GGYRCC 2 cut(s) 526, 781
BsiEI CGRYCG 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 487
BsiSI CCGG 2 cut(s) 252, 264
BslI CCNNNNNNNGG 1 cut(s) 281
BsmAI GTCTC 1 cut(s) 739
BsnI GGCC 2 cut(s) 251, 374
Bsp1286I GDGCHC 1 cut(s) 487
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 4 cut(s) 181, 546, 680, 773
BspANI GGCC 2 cut(s) 251, 374
BspCNI CTCAG 4 cut(s) 423, 480, 685, 735
BspHI TCATGA 1 cut(s) 718
BspLI GGNNCC 3 cut(s) 528, 672, 783
BspMAI CTGCAG 1 cut(s) 773
BspPI GGATC 3 cut(s) 176, 688, 768
BspT107I GGYRCC 2 cut(s) 526, 781
BsrGI TGTACA 1 cut(s) 306
BssECI CCNNGG 3 cut(s) 124, 185, 777
BssMI GATC 4 cut(s) 181, 546, 680, 773
BssSI CACGAG 1 cut(s) 17
Bst2BI CACGAG 1 cut(s) 17
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 661
Bst6I CTCTTC 1 cut(s) 557
BstAPI GCANNNNNTGC 1 cut(s) 423
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 227, 269
BstDEI CTNAG 5 cut(s) 431, 488, 693, 743, 749
BstDSI CCRYGG 1 cut(s) 124
BstF5I GGATG 2 cut(s) 328, 743
BstKTI GATC 4 cut(s) 184, 549, 683, 776
BstMAI GTCTC 1 cut(s) 739
BstMBI GATC 4 cut(s) 181, 546, 680, 773
BstMCI CGRYCG 1 cut(s) 252
BstMWI GCNNNNNNNGC 1 cut(s) 423
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 2 cut(s) 229, 713
BstSCI CCNGG 2 cut(s) 184, 251
BstSFI CTRYAG 1 cut(s) 769
BstV1I GCAGC 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 2 cut(s) 181, 773
BstXI CCANNNNNNTGG 1 cut(s) 185
BstYI RGATCY 2 cut(s) 181, 773
BstZI CGGCCG 1 cut(s) 249
BsuRI GGCC 2 cut(s) 251, 374
BtgI CCRYGG 1 cut(s) 124
BtsCI GGATG 2 cut(s) 328, 743
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 1 cut(s) 412
Cac8I GCNNGC 2 cut(s) 227, 269
CciI TCATGA 1 cut(s) 718
Csp6I GTAC 6 cut(s) 297, 307, 383, 527, 597, 657
CviAII CATG 3 cut(s) 226, 710, 719
CviQI GTAC 6 cut(s) 297, 307, 383, 527, 597, 657
DdeI CTNAG 5 cut(s) 431, 488, 693, 743, 749
DpnI GATC 4 cut(s) 183, 548, 682, 775
DpnII GATC 4 cut(s) 181, 546, 680, 773
EaeI YGGCCR 1 cut(s) 249
EagI CGGCCG 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 557
EarI CTCTTC 1 cut(s) 557
Ecl136II GAGCTC 1 cut(s) 485
EclXI CGGCCG 1 cut(s) 249
Eco24I GRGCYC 1 cut(s) 487
Eco52I CGGCCG 1 cut(s) 249
Eco53kI GAGCTC 1 cut(s) 485
EcoICRI GAGCTC 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 184
EcoT38I GRGCYC 1 cut(s) 487
FaeI CATG 3 cut(s) 229, 713, 722
FalI AAGNNNNNCTT 2 cut(s) 78, 110
FatI CATG 3 cut(s) 225, 709, 718
FauNDI CATATG 1 cut(s) 685
FblI GTMKAC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 272
FokI GGATG 2 cut(s) 335, 750
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 1 cut(s) 272
FspBI CTAG 4 cut(s) 54, 68, 146, 618
GluI GCNGC 1 cut(s) 272
HaeIII GGCC 2 cut(s) 251, 374
HapII CCGG 2 cut(s) 252, 264
Hin1II CATG 3 cut(s) 229, 713, 722
HinfI GANTC 7 cut(s) 13, 191, 285, 365, 409, 494, 539
HpaII CCGG 2 cut(s) 252, 264
Hpy166II GTNNAC 2 cut(s) 112, 764
Hpy188I TCNGA 4 cut(s) 432, 489, 499, 778
Hpy188III TCNNGA 4 cut(s) 17, 362, 550, 719
Hpy8I GTNNAC 2 cut(s) 112, 764
HpyCH4III ACNGT 1 cut(s) 661
HpyCH4V TGCA 5 cut(s) 208, 417, 705, 729, 771
HpyF10VI GCNNNNNNNGC 1 cut(s) 423
HpyF3I CTNAG 5 cut(s) 431, 488, 693, 743, 749
Hsp92II CATG 3 cut(s) 229, 713, 722
KpnI GGTACC 1 cut(s) 530
Kzo9I GATC 4 cut(s) 181, 546, 680, 773
LmnI GCTCC 1 cut(s) 670
Lsp1109I GCAGC 1 cut(s) 258
LweI GCATC 2 cut(s) 313, 687
MaeI CTAG 4 cut(s) 54, 68, 146, 618
MaeIII GTNAC 2 cut(s) 38, 752
MalI GATC 4 cut(s) 183, 548, 682, 775
MboI GATC 4 cut(s) 181, 546, 680, 773
MboII GAAGA 6 cut(s) 112, 208, 526, 574, 621, 734
MflI RGATCY 2 cut(s) 181, 773
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 3 cut(s) 139, 455, 790
MlyI GAGTC 3 cut(s) 200, 374, 548
MslI CAYNNNNRTG 2 cut(s) 129, 213
MspI CCGG 2 cut(s) 252, 264
MspR9I CCNGG 2 cut(s) 186, 253
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 1 cut(s) 423
NciI CCSGG 1 cut(s) 253
NdeI CATATG 1 cut(s) 685
NdeII GATC 4 cut(s) 181, 546, 680, 773
NlaIII CATG 3 cut(s) 229, 713, 722
NlaIV GGNNCC 3 cut(s) 528, 672, 783
NmeAIII GCCGAG 1 cut(s) 529
NmuCI GTSAC 2 cut(s) 38, 752
NspI RCATGY 2 cut(s) 229, 713
PaeI GCATGC 1 cut(s) 229
PagI TCATGA 1 cut(s) 718
PfeI GAWTC 4 cut(s) 13, 285, 409, 494
PflFI GACNNNGTC 1 cut(s) 750
PkrI GCNGC 1 cut(s) 273
PleI GAGTC 3 cut(s) 199, 373, 547
PpsI GAGTC 3 cut(s) 199, 373, 547
Psp124BI GAGCTC 1 cut(s) 487
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 528, 672, 783
PstI CTGCAG 1 cut(s) 773
PsuI RGATCY 2 cut(s) 181, 773
PsyI GACNNNGTC 1 cut(s) 750
RsaI GTAC 6 cut(s) 298, 308, 384, 528, 598, 658
RsaNI GTAC 6 cut(s) 297, 307, 383, 527, 597, 657
RseI CAYNNNNRTG 2 cut(s) 129, 213
SacI GAGCTC 1 cut(s) 487
SatI GCNGC 1 cut(s) 272
Sau3AI GATC 4 cut(s) 181, 546, 680, 773
SchI GAGTC 3 cut(s) 200, 374, 548
ScrFI CCNGG 2 cut(s) 186, 253
SduI GDGCHC 1 cut(s) 487
SetI ASST 7 cut(s) 78, 298, 453, 487, 558, 639, 783
SfaNI GCATC 2 cut(s) 313, 687
SfcI CTRYAG 1 cut(s) 769
SmiMI CAYNNNNRTG 2 cut(s) 129, 213
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
SphI GCATGC 1 cut(s) 229
Sse9I AATT 3 cut(s) 139, 455, 790
SspMI CTAG 4 cut(s) 54, 68, 146, 618
SstI GAGCTC 1 cut(s) 487
StyD4I CCNGG 2 cut(s) 184, 251
TaaI ACNGT 1 cut(s) 661
TaqI TCGA 3 cut(s) 33, 194, 576
TasI AATT 3 cut(s) 139, 455, 790
TatI WGTACW 3 cut(s) 306, 596, 656
TfiI GAWTC 4 cut(s) 13, 285, 409, 494
TscAI CASTG 1 cut(s) 419
TseFI GTSAC 2 cut(s) 38, 752
TseI GCWGC 1 cut(s) 271
Tsp45I GTSAC 2 cut(s) 38, 752
TspDTI ATGAA 4 cut(s) 68, 527, 707, 735
TspRI CASTG 1 cut(s) 419
Tth111I GACNNNGTC 1 cut(s) 750
XceI RCATGY 2 cut(s) 229, 713
XmiI GTMKAC 1 cut(s) 111
XspI CTAG 4 cut(s) 54, 68, 146, 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.