FvH4_4g31940

DUF1338

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0010892
Physical Location & Seq
Forward (+)
30830129 .. 30832999
2871 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g31940.t1

Sequence Viewer

Length: 1143 bp
ATGATTACTCGACTCTCCACCACCACCATGACTATGTTAAGAAGCTCCTCTGTTGCATCCCCATCACTTCACTCTCTCATTCCTCGCCAACCCAACAATCTCAATCCCACCAGGGACTTCATCTCCCTCACCAAATCACCTCTTCCCCTGAATCTCAAACGCTCAGTCTCCACCAACAAGCATACTCTCCGCACCCTTTGTAGCTCCAAACCAGACAATGGATCCCATCATCCTTCTTCTTCTCAGGGAGGCGAGTCATTCTTCAGGGGAGTGTTGGCAAACATGGAGGCTGTGTACTTGAGCAGGAACCCTACAGCAAAGTCAGTGTTGGAGCTTGTCGGGTCTGATGAACAGATTAGCTATGATCATTTTGCGTTTAGGACCTTTGGGGTGAATGGTTATGGGATTGATTCCATTGCTAGATTTTTCTTGGATTTCGGGTACACTACCCGGGAGGAATTACGATTCCCGGCTAAGAAATTGAGGGCACTGTGGTTTTCTCCTCCTAGTAATGCCACTTCTGATGGTGGTGCTGGTGTTAATGGGCCCTTGCCGAGAATATTCATATCTGAGCTTCTTGTAGATCAGATGAGTCCACAGACTCAGATAATCCATAAGTACACTGAAAACTCTGGTTCTGGAAATAAGTATGCTGCTCTAGCAAGTGCGCTGGGAACTTTGACTTGGGAAAAGCCGTTGTACACAGAATTTCAGCAATTGGCTAGGGAAAGTGAATATGCTGCCTGGACCCTTGTCAATGGGTATGCAGTCAACCATGTCACTATCTCTGCTCACCAGCTGAAATCTCAATTGAGAGATATTAAAAGCCTCAATCAGTTTATCGAGAAGAATGGATTCAGGTTGAATTCTGAAGGTGGAATTCTGAAAGTGAGCCCTGATGGTCTTCTGCTGCAAAGTTCAACGGTCGCAGATTCGGTTCATTTCCAATTTAGGGATGGTCAAATCGAGTTAGTCCCCTGCTCGTACATTGAGTTTGCTCAACGCCTGGTGCTGCCTCAGTACAGTGATCTACCAAGCAATGAGGTTAAAGAGCATCACAGGAGGGATGGATTTGAAGTTGGTAATGCTGATAAGATATTTGAGAGCACATCAAAGGAGCAGCTGACCAGGAGGGCTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1338

Protein Analysis

381

Amino Acids

42.27

Weight (kDa)

8.91

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGLS PF07063 107 - 338 2.8e-41 2-oxoadipate dioxygenase/decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 218
AciI CCGC 1 cut(s) 190
AclWI GGATC 2 cut(s) 216, 229
AcsI RAATTY 3 cut(s) 707, 865, 879
AcuI CTGAAG 2 cut(s) 247, 891
AfaI GTAC 6 cut(s) 296, 443, 620, 701, 986, 1022
AfiI CCNNNNNNNGG 2 cut(s) 218, 952
AgsI TTSAA 3 cut(s) 865, 921, 1076
AjnI CCWGG 4 cut(s) 110, 743, 1005, 1127
AjuI GAANNNNNNNTTGG 2 cut(s) 667, 699
AluBI AGCT 7 cut(s) 45, 204, 334, 360, 574, 799, 1123
AluI AGCT 7 cut(s) 45, 204, 334, 360, 574, 799, 1123
Alw21I GWGCWC 1 cut(s) 1109
Alw26I GTCTC 1 cut(s) 172
AlwI GGATC 2 cut(s) 216, 229
Ama87I CYCGRG 1 cut(s) 450
AoxI GGCC 1 cut(s) 545
ApaI GGGCCC 1 cut(s) 549
ApeKI GCWGC 6 cut(s) 653, 740, 910, 1012, 1120, 1136
ApoI RAATTY 3 cut(s) 707, 865, 879
ArsI GACNNNNNNTTYG 2 cut(s) 150, 182
Asp700I GAANNNNTTC 1 cut(s) 854
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 4 cut(s) 381, 545, 546, 747
AsuC2I CCSGG 3 cut(s) 451, 452, 470
AsuHPI GGTGA 4 cut(s) 121, 129, 403, 785
AvaI CYCGRG 1 cut(s) 450
AvaII GGWCC 2 cut(s) 381, 747
BaeGI GKGCMC 2 cut(s) 490, 549
BamHI GGATCC 1 cut(s) 221
BanII GRGCYC 2 cut(s) 549, 896
BarI GAAGNNNNNNTAC 2 cut(s) 502, 534
BbsI GAAGAC 1 cut(s) 896
Bbv12I GWGCWC 1 cut(s) 1109
BbvI GCAGC 6 cut(s) 640, 727, 897, 999, 1123, 1132
BccI CCATC 6 cut(s) 70, 234, 518, 893, 950, 1061
BceAI ACGGC 1 cut(s) 679
BciT130I CCWGG 4 cut(s) 112, 745, 1007, 1129
BclI TGATCA 1 cut(s) 364
BcnI CCSGG 3 cut(s) 451, 452, 470
BcoDI GTCTC 1 cut(s) 172
BfaI CTAG 4 cut(s) 420, 507, 659, 723
BfmI CTRYAG 1 cut(s) 312
BisI GCNGC 6 cut(s) 654, 741, 911, 1013, 1121, 1137
BlsI GCNGC 6 cut(s) 655, 742, 912, 1014, 1122, 1138
Bme1390I CCNGG 7 cut(s) 112, 451, 452, 470, 745, 1007, 1129
Bme18I GGWCC 2 cut(s) 381, 747
BmeT110I CYCGRG 1 cut(s) 450
BmgT120I GGNCC 4 cut(s) 381, 545, 546, 747
BmiI GGNNCC 4 cut(s) 223, 308, 547, 749
BmrFI CCNGG 7 cut(s) 112, 451, 452, 470, 745, 1007, 1129
BmsI GCATC 2 cut(s) 65, 1063
BoxI GACNNNNGTC 1 cut(s) 752
BpiI GAAGAC 1 cut(s) 896
BpuEI CTTGAG 1 cut(s) 319
BpuMI CCSGG 3 cut(s) 451, 452, 470
BsaJI CCNNGG 2 cut(s) 111, 450
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 278, 308
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 952
Bse3DI GCAATG 2 cut(s) 414, 1045
BseBI CCWGG 4 cut(s) 112, 745, 1007, 1129
BseDI CCNNGG 2 cut(s) 111, 450
BseGI GGATG 4 cut(s) 56, 229, 961, 1072
BseLI CCNNNNNNNGG 2 cut(s) 218, 952
BseMI GCAATG 2 cut(s) 414, 1045
BseMII CTCAG 5 cut(s) 177, 257, 561, 617, 1031
BseRI GAGGAG 2 cut(s) 37, 492
BseSI GKGCMC 2 cut(s) 490, 549
BseXI GCAGC 6 cut(s) 640, 727, 897, 999, 1123, 1132
BseYI CCCAGC 1 cut(s) 670
Bsh1285I CGRYCG 1 cut(s) 927
BshFI GGCC 1 cut(s) 547
BsiEI CGRYCG 1 cut(s) 927
BsiHKAI GWGCWC 1 cut(s) 1109
BsiHKCI CYCGRG 1 cut(s) 450
BsiSI CCGG 2 cut(s) 451, 470
BslFI GGGAC 2 cut(s) 128, 959
BslI CCNNNNNNNGG 2 cut(s) 218, 952
BsmAI GTCTC 1 cut(s) 172
BsmFI GGGAC 2 cut(s) 128, 959
BsnI GGCC 1 cut(s) 547
BsoBI CYCGRG 1 cut(s) 450
Bsp120I GGGCCC 1 cut(s) 545
Bsp1286I GDGCHC 4 cut(s) 490, 549, 896, 1109
Bsp1407I TGTACA 1 cut(s) 699
Bsp143I GATC 4 cut(s) 221, 364, 583, 1027
BspACI CCGC 1 cut(s) 190
BspANI GGCC 1 cut(s) 547
BspCNI CTCAG 5 cut(s) 176, 256, 562, 616, 1030
BspLI GGNNCC 4 cut(s) 223, 308, 547, 749
BspPI GGATC 2 cut(s) 216, 229
BsrDI GCAATG 2 cut(s) 414, 1045
BsrGI TGTACA 1 cut(s) 699
BssECI CCNNGG 2 cut(s) 111, 450
BssMI GATC 4 cut(s) 221, 364, 583, 1027
Bst2UI CCWGG 4 cut(s) 112, 745, 1007, 1129
Bst4CI ACNGT 3 cut(s) 492, 925, 1025
Bst6I CTCTTC 1 cut(s) 147
BstAUI TGTACA 1 cut(s) 699
BstDEI CTNAG 6 cut(s) 163, 243, 474, 570, 603, 1017
BstF5I GGATG 4 cut(s) 56, 229, 961, 1072
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 4 cut(s) 224, 367, 586, 1030
BstMAI GTCTC 1 cut(s) 172
BstMBI GATC 4 cut(s) 221, 364, 583, 1027
BstMCI CGRYCG 1 cut(s) 927
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 4 cut(s) 112, 745, 1007, 1129
BstPAI GACNNNNGTC 1 cut(s) 752
BstSCI CCNGG 7 cut(s) 110, 449, 450, 468, 743, 1005, 1127
BstSFI CTRYAG 1 cut(s) 312
BstSLI GKGCMC 2 cut(s) 490, 549
BstV1I GCAGC 6 cut(s) 640, 727, 897, 999, 1123, 1132
BstV2I GAAGAC 1 cut(s) 896
BstX2I RGATCY 1 cut(s) 221
BstYI RGATCY 1 cut(s) 221
BsuRI GGCC 1 cut(s) 547
BtsCI GGATG 4 cut(s) 56, 229, 961, 1072
BtsIMutI CAGTG 4 cut(s) 330, 488, 621, 1030
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 4 cut(s) 381, 545, 546, 747
Cfr9I CCCGGG 1 cut(s) 450
Csp6I GTAC 6 cut(s) 295, 442, 619, 700, 985, 1021
CviAII CATG 3 cut(s) 28, 283, 776
CviQI GTAC 6 cut(s) 295, 442, 619, 700, 985, 1021
DdeI CTNAG 6 cut(s) 163, 243, 474, 570, 603, 1017
DpnI GATC 4 cut(s) 223, 366, 585, 1029
DpnII GATC 4 cut(s) 221, 364, 583, 1027
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
Eco24I GRGCYC 2 cut(s) 549, 896
Eco47I GGWCC 2 cut(s) 381, 747
Eco57I CTGAAG 2 cut(s) 247, 891
Eco88I CYCGRG 1 cut(s) 450
EcoO109I RGGNCCY 2 cut(s) 381, 546
EcoRI GAATTC 2 cut(s) 865, 879
EcoRII CCWGG 4 cut(s) 110, 743, 1005, 1127
EcoT38I GRGCYC 2 cut(s) 549, 896
FaeI CATG 3 cut(s) 31, 286, 779
FaqI GGGAC 2 cut(s) 128, 959
FatI CATG 3 cut(s) 27, 282, 775
FbaI TGATCA 1 cut(s) 364
Fnu4HI GCNGC 6 cut(s) 654, 741, 911, 1013, 1121, 1137
FokI GGATG 4 cut(s) 43, 216, 968, 1079
FriOI GRGCYC 2 cut(s) 549, 896
Fsp4HI GCNGC 6 cut(s) 654, 741, 911, 1013, 1121, 1137
FspBI CTAG 4 cut(s) 420, 507, 659, 723
GlaI GCGC 1 cut(s) 669
GluI GCNGC 6 cut(s) 654, 741, 911, 1013, 1121, 1137
GsaI CCCAGC 1 cut(s) 674
HaeIII GGCC 1 cut(s) 547
HapII CCGG 2 cut(s) 451, 470
HhaI GCGC 1 cut(s) 670
Hin1II CATG 3 cut(s) 31, 286, 779
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 9 cut(s) 12, 151, 254, 410, 465, 592, 601, 855, 932
HpaII CCGG 2 cut(s) 451, 470
HphI GGTGA 4 cut(s) 121, 129, 403, 785
Hpy166II GTNNAC 6 cut(s) 295, 444, 596, 621, 702, 772
Hpy188I TCNGA 7 cut(s) 346, 523, 571, 588, 606, 871, 885
Hpy188III TCNNGA 2 cut(s) 639, 844
Hpy8I GTNNAC 6 cut(s) 295, 444, 596, 621, 702, 772
HpyAV CCTTC 2 cut(s) 243, 866
HpyCH4III ACNGT 3 cut(s) 492, 925, 1025
HpyCH4V TGCA 3 cut(s) 56, 767, 913
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 6 cut(s) 163, 243, 474, 570, 603, 1017
Hsp92II CATG 3 cut(s) 31, 286, 779
HspAI GCGC 1 cut(s) 668
Ksp22I TGATCA 1 cut(s) 364
Kzo9I GATC 4 cut(s) 221, 364, 583, 1027
LmnI GCTCC 4 cut(s) 50, 209, 331, 1117
Lsp1109I GCAGC 6 cut(s) 640, 727, 897, 999, 1123, 1132
LweI GCATC 2 cut(s) 65, 1063
MaeI CTAG 4 cut(s) 420, 507, 659, 723
MaeIII GTNAC 1 cut(s) 778
MalI GATC 4 cut(s) 223, 366, 585, 1029
MboI GATC 4 cut(s) 221, 364, 583, 1027
MboII GAAGA 6 cut(s) 134, 228, 231, 253, 859, 896
MfeI CAATTG 2 cut(s) 716, 809
MflI RGATCY 1 cut(s) 221
MhlI GDGCHC 4 cut(s) 490, 549, 896, 1109
MluCI AATT 8 cut(s) 458, 479, 707, 716, 809, 865, 879, 947
MlyI GAGTC 4 cut(s) 6, 263, 595, 601
MmeI TCCRAC 1 cut(s) 309
MroXI GAANNNNTTC 1 cut(s) 854
MseI TTAA 4 cut(s) 38, 540, 822, 1047
MslI CAYNNNNRTG 2 cut(s) 26, 32
MspA1I CMGCKG 2 cut(s) 799, 1123
MspI CCGG 2 cut(s) 451, 470
MspR9I CCNGG 7 cut(s) 112, 451, 452, 470, 745, 1007, 1129
MunI CAATTG 2 cut(s) 716, 809
MvaI CCWGG 4 cut(s) 112, 745, 1007, 1129
MwoI GCNNNNNNNGC 1 cut(s) 659
NciI CCSGG 3 cut(s) 451, 452, 470
NdeII GATC 4 cut(s) 221, 364, 583, 1027
NlaIII CATG 3 cut(s) 31, 286, 779
NlaIV GGNNCC 4 cut(s) 223, 308, 547, 749
NmeAIII GCCGAG 1 cut(s) 579
NmuCI GTSAC 1 cut(s) 778
PdmI GAANNNNTTC 1 cut(s) 854
PfeI GAWTC 5 cut(s) 151, 410, 465, 855, 932
PflMI CCANNNNNTGG 1 cut(s) 218
PkrI GCNGC 6 cut(s) 655, 742, 912, 1014, 1122, 1138
PleI GAGTC 4 cut(s) 6, 262, 595, 600
PpsI GAGTC 4 cut(s) 6, 262, 595, 600
PpuMI RGGWCCY 1 cut(s) 381
PshAI GACNNNNGTC 1 cut(s) 752
Psp5II RGGWCCY 1 cut(s) 381
Psp6I CCWGG 4 cut(s) 110, 743, 1005, 1127
PspFI CCCAGC 1 cut(s) 670
PspGI CCWGG 4 cut(s) 110, 743, 1005, 1127
PspN4I GGNNCC 4 cut(s) 223, 308, 547, 749
PspOMI GGGCCC 1 cut(s) 545
PspPI GGNCC 4 cut(s) 381, 545, 546, 747
PspPPI RGGWCCY 1 cut(s) 381
PsuI RGATCY 1 cut(s) 221
PvuII CAGCTG 2 cut(s) 799, 1123
RsaI GTAC 6 cut(s) 296, 443, 620, 701, 986, 1022
RsaNI GTAC 6 cut(s) 295, 442, 619, 700, 985, 1021
RseI CAYNNNNRTG 2 cut(s) 26, 32
SaqAI TTAA 4 cut(s) 38, 540, 822, 1047
SatI GCNGC 6 cut(s) 654, 741, 911, 1013, 1121, 1137
Sau3AI GATC 4 cut(s) 221, 364, 583, 1027
Sau96I GGNCC 4 cut(s) 381, 545, 546, 747
SchI GAGTC 4 cut(s) 6, 263, 595, 601
ScrFI CCNGG 7 cut(s) 112, 451, 452, 470, 745, 1007, 1129
SduI GDGCHC 4 cut(s) 490, 549, 896, 1109
SfaNI GCATC 2 cut(s) 65, 1063
SfcI CTRYAG 1 cut(s) 312
SinI GGWCC 2 cut(s) 381, 747
SmaI CCCGGG 1 cut(s) 452
SmiMI CAYNNNNRTG 2 cut(s) 26, 32
SmlI CTYRAG 1 cut(s) 298
SmoI CTYRAG 1 cut(s) 298
Sse9I AATT 8 cut(s) 458, 479, 707, 716, 809, 865, 879, 947
SsiI CCGC 1 cut(s) 190
SspI AATATT 1 cut(s) 561
SspMI CTAG 4 cut(s) 420, 507, 659, 723
StyD4I CCNGG 7 cut(s) 110, 449, 450, 468, 743, 1005, 1127
TaaI ACNGT 3 cut(s) 492, 925, 1025
TaqI TCGA 3 cut(s) 10, 843, 966
TasI AATT 8 cut(s) 458, 479, 707, 716, 809, 865, 879, 947
TatI WGTACW 4 cut(s) 294, 618, 699, 1020
TfiI GAWTC 5 cut(s) 151, 410, 465, 855, 932
Tru1I TTAA 4 cut(s) 38, 540, 822, 1047
Tru9I TTAA 4 cut(s) 38, 540, 822, 1047
TscAI CASTG 4 cut(s) 330, 495, 628, 1030
TseFI GTSAC 1 cut(s) 778
TseI GCWGC 6 cut(s) 653, 740, 910, 1012, 1120, 1136
Tsp45I GTSAC 1 cut(s) 778
TspDTI ATGAA 4 cut(s) 109, 363, 553, 929
TspMI CCCGGG 1 cut(s) 450
TspRI CASTG 4 cut(s) 330, 495, 628, 1030
Van91I CCANNNNNTGG 1 cut(s) 218
VpaK11BI GGWCC 2 cut(s) 381, 747
XapI RAATTY 3 cut(s) 707, 865, 879
XcmI CCANNNNNNNNNTGG 1 cut(s) 953
XmaI CCCGGG 1 cut(s) 450
XmnI GAANNNNTTC 1 cut(s) 854
XspI CTAG 4 cut(s) 420, 507, 659, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.