Rw4G032530

DUF1338

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0010892
Physical Location & Seq
Forward (+)
59624546 .. 59626892
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G032530.1

Sequence Viewer

Length: 996 bp
ATGATTACCCGATTCTCCACCACCATGACCATGTTAAGAAGCTCCTCTATCTCCTCCCCATCACTTGACTCTCTCATTCCTCGCCAACCCAACAGTGGAGGTGAATCATTCTTCAGGGGAGTGTTGGCAAACATGGAGGCTGTGTACTTGAGCAGGAACCCTACAGCAAAGTCAGTGTTGGAGCTTGTTGGGTCCGATGAACAGATTAGCTATGATCATTTTGCATTCAGGACCTTTGGGGTGAATGGTTATGGGATTGATTCCATGGCTAGATTTTTCTTGGATTTCGGGTACACTCCCCGGGAGGAGTTACGATTCCCGGCTAAGAAATTGAGGGCATTCTGGTTTGCTCCTCCTAGTTATACCATTTCTGATGGTGGTGCTGGTGTTAATGGGCCTTTGCCGAGAATATTTATATCTGAGCTTCTTGTAGATCAGATGAGTCCACAAACTCAGGAGATAATCCGTAAGTACACTGAAAACTCTGGTTCTGGAAATAAGTATGCTGCTCTAGCAAGTGCGCTGGGAACTTTGACGTGGGAAAAGCCGTTGTACTCAGAATTTCAGCAATTGGCTAGGGAAAGTGAATATGCTGCCTGGACCCTTGTCAATGGGTATGCAGTCAACCATGTCACAATCTCTGCTCACCAGCTGAAATCTAAATTGAGAGATATTAAAAGCCTCAATCAATTCATCGAGGAGAATGGGTTCAGGTTGAATTCTGAAGGCGGAATTCTGAAAGTGAGCCCCGATGGTCTTCTGCTGCAAAGTTCAACTGTCGCAGATCCAGTTAATTTCCAATTTAGGGATGGTCAAATTGAGTCAGTCCCCTGCTCCTACATTGAGTTTGCTCAACGCCTGGTGCTGCCCCAGTACTGCAATCTACCAAGCAACGAGGTTAAAGAGCATCACAGGCGAGATGGCTTTGAGGTTGGTAATGCGGACAAGATCTTTGAGAGCACATCAAAGGAGCAGCTGACCAGGAGGGCTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1338

Protein Analysis

331

Amino Acids

37.03

Weight (kDa)

6.26

Isoelectric Point (pI)

43.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGLS PF07063 57 - 289 6.6e-43 2-oxoadipate dioxygenase/decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 729, 941
AclWI GGATC 1 cut(s) 779
AcsI RAATTY 3 cut(s) 560, 718, 732
AcuI CTGAAG 2 cut(s) 97, 744
AfaI GTAC 5 cut(s) 146, 293, 473, 554, 875
AfiI CCNNNNNNNGG 2 cut(s) 95, 805
AgsI TTSAA 2 cut(s) 718, 774
AjiI CACGTC 1 cut(s) 537
AjnI CCWGG 3 cut(s) 596, 858, 980
AloI GAACNNNNNNTCC 2 cut(s) 692, 724
AluBI AGCT 6 cut(s) 42, 184, 210, 424, 652, 976
AluI AGCT 6 cut(s) 42, 184, 210, 424, 652, 976
Alw21I GWGCWC 1 cut(s) 962
AlwI GGATC 1 cut(s) 779
Ama87I CYCGRG 1 cut(s) 300
AoxI GGCC 1 cut(s) 395
ApeKI GCWGC 6 cut(s) 506, 593, 763, 865, 973, 989
ApoI RAATTY 3 cut(s) 560, 718, 732
ArsI GACNNNNNNTTYG 2 cut(s) 935, 967
Asp700I GAANNNNTTC 1 cut(s) 707
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 4 cut(s) 192, 231, 395, 600
AsuC2I CCSGG 3 cut(s) 301, 302, 320
AsuHPI GGTGA 3 cut(s) 113, 253, 638
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 3 cut(s) 192, 231, 600
BanII GRGCYC 1 cut(s) 749
BbsI GAAGAC 1 cut(s) 749
Bbv12I GWGCWC 1 cut(s) 962
BbvI GCAGC 6 cut(s) 493, 580, 750, 852, 976, 985
BccI CCATC 5 cut(s) 67, 368, 746, 803, 914
BceAI ACGGC 1 cut(s) 532
BciT130I CCWGG 3 cut(s) 598, 860, 982
BclI TGATCA 1 cut(s) 214
BcnI CCSGG 3 cut(s) 301, 302, 320
BfaI CTAG 4 cut(s) 270, 357, 512, 576
BfmI CTRYAG 1 cut(s) 162
BglII AGATCT 1 cut(s) 948
BisI GCNGC 6 cut(s) 507, 594, 764, 866, 974, 990
BlsI GCNGC 6 cut(s) 508, 595, 765, 867, 975, 991
BmcAI AGTACT 1 cut(s) 875
Bme1390I CCNGG 6 cut(s) 301, 302, 320, 598, 860, 982
Bme18I GGWCC 3 cut(s) 192, 231, 600
BmeT110I CYCGRG 1 cut(s) 300
BmgBI CACGTC 1 cut(s) 537
BmgT120I GGNCC 4 cut(s) 192, 231, 395, 600
BmiI GGNNCC 3 cut(s) 158, 193, 602
BmrFI CCNGG 6 cut(s) 301, 302, 320, 598, 860, 982
BmrI ACTGGG 1 cut(s) 865
BmsI GCATC 1 cut(s) 916
BmuI ACTGGG 1 cut(s) 865
BoxI GACNNNNGTC 1 cut(s) 605
BpiI GAAGAC 1 cut(s) 749
BpuEI CTTGAG 1 cut(s) 169
BpuMI CCSGG 3 cut(s) 301, 302, 320
BsaJI CCNNGG 3 cut(s) 264, 299, 300
BsaXI ACNNNNNCTCC 4 cut(s) 128, 158, 692, 722
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 805
Bse1I ACTGG 2 cut(s) 788, 871
BseBI CCWGG 3 cut(s) 598, 860, 982
BseDI CCNNGG 3 cut(s) 264, 299, 300
BseGI GGATG 1 cut(s) 814
BseLI CCNNNNNNNGG 2 cut(s) 95, 805
BseMII CTCAG 3 cut(s) 411, 467, 570
BseNI ACTGG 2 cut(s) 788, 871
BseRI GAGGAG 5 cut(s) 34, 43, 320, 342, 713
BseXI GCAGC 6 cut(s) 493, 580, 750, 852, 976, 985
BseYI CCCAGC 1 cut(s) 523
BshFI GGCC 1 cut(s) 397
BsiHKAI GWGCWC 1 cut(s) 962
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 2 cut(s) 301, 320
BslFI GGGAC 1 cut(s) 812
BslI CCNNNNNNNGG 2 cut(s) 95, 805
BsmFI GGGAC 1 cut(s) 812
BsmI GAATGC 2 cut(s) 224, 338
BsnI GGCC 1 cut(s) 397
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 2 cut(s) 749, 962
Bsp143I GATC 4 cut(s) 214, 433, 784, 948
Bsp19I CCATGG 1 cut(s) 264
BspACI CCGC 2 cut(s) 729, 941
BspANI GGCC 1 cut(s) 397
BspCNI CTCAG 3 cut(s) 412, 466, 569
BspLI GGNNCC 3 cut(s) 158, 193, 602
BspPI GGATC 1 cut(s) 779
BsrI ACTGG 2 cut(s) 788, 871
BssECI CCNNGG 3 cut(s) 264, 299, 300
BssMI GATC 4 cut(s) 214, 433, 784, 948
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 3 cut(s) 598, 860, 982
Bst4CI ACNGT 2 cut(s) 95, 778
BstDEI CTNAG 4 cut(s) 324, 420, 453, 556
BstDSI CCRYGG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 814
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 4 cut(s) 217, 436, 787, 951
BstMBI GATC 4 cut(s) 214, 433, 784, 948
BstMWI GCNNNNNNNGC 2 cut(s) 512, 913
BstNI CCWGG 3 cut(s) 598, 860, 982
BstPAI GACNNNNGTC 1 cut(s) 605
BstSCI CCNGG 6 cut(s) 299, 300, 318, 596, 858, 980
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 6 cut(s) 493, 580, 750, 852, 976, 985
BstV2I GAAGAC 1 cut(s) 749
BstX2I RGATCY 2 cut(s) 784, 948
BstYI RGATCY 2 cut(s) 784, 948
BsuRI GGCC 1 cut(s) 397
BtgI CCRYGG 1 cut(s) 264
BtrI CACGTC 1 cut(s) 537
BtsCI GGATG 1 cut(s) 814
BtsIMutI CAGTG 3 cut(s) 100, 180, 474
CfoI GCGC 1 cut(s) 523
Cfr13I GGNCC 4 cut(s) 192, 231, 395, 600
Cfr9I CCCGGG 1 cut(s) 300
Csp6I GTAC 5 cut(s) 145, 292, 472, 553, 874
CviAII CATG 6 cut(s) 25, 31, 133, 265, 629, 993
CviQI GTAC 5 cut(s) 145, 292, 472, 553, 874
DdeI CTNAG 4 cut(s) 324, 420, 453, 556
DpnI GATC 4 cut(s) 216, 435, 786, 950
DpnII GATC 4 cut(s) 214, 433, 784, 948
EciI GGCGGA 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 749
Eco47I GGWCC 3 cut(s) 192, 231, 600
Eco57I CTGAAG 2 cut(s) 97, 744
Eco88I CYCGRG 1 cut(s) 300
EcoO109I RGGNCCY 1 cut(s) 231
EcoRI GAATTC 2 cut(s) 718, 732
EcoRII CCWGG 3 cut(s) 596, 858, 980
EcoT14I CCWWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 749
ErhI CCWWGG 1 cut(s) 264
FaeI CATG 6 cut(s) 28, 34, 136, 268, 632, 996
FaqI GGGAC 1 cut(s) 812
FatI CATG 6 cut(s) 24, 30, 132, 264, 628, 992
FbaI TGATCA 1 cut(s) 214
Fnu4HI GCNGC 6 cut(s) 507, 594, 764, 866, 974, 990
FokI GGATG 1 cut(s) 821
FriOI GRGCYC 1 cut(s) 749
Fsp4HI GCNGC 6 cut(s) 507, 594, 764, 866, 974, 990
FspBI CTAG 4 cut(s) 270, 357, 512, 576
GlaI GCGC 1 cut(s) 522
GluI GCNGC 6 cut(s) 507, 594, 764, 866, 974, 990
GsaI CCCAGC 1 cut(s) 527
HaeIII GGCC 1 cut(s) 397
HapII CCGG 2 cut(s) 301, 320
HhaI GCGC 1 cut(s) 523
Hin1II CATG 6 cut(s) 28, 34, 136, 268, 632, 996
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HincII GTYRAC 1 cut(s) 625
HindII GTYRAC 1 cut(s) 625
HinfI GANTC 7 cut(s) 12, 68, 104, 260, 315, 442, 821
HpaII CCGG 2 cut(s) 301, 320
HphI GGTGA 3 cut(s) 113, 253, 638
Hpy166II GTNNAC 5 cut(s) 145, 294, 446, 474, 625
Hpy188I TCNGA 7 cut(s) 196, 373, 421, 438, 559, 724, 738
Hpy188III TCNNGA 3 cut(s) 229, 455, 492
Hpy8I GTNNAC 5 cut(s) 145, 294, 446, 474, 625
HpyAV CCTTC 1 cut(s) 719
HpyCH4III ACNGT 2 cut(s) 95, 778
HpyCH4IV ACGT 1 cut(s) 536
HpyCH4V TGCA 5 cut(s) 224, 620, 766, 879, 992
HpyF10VI GCNNNNNNNGC 2 cut(s) 512, 913
HpyF3I CTNAG 4 cut(s) 324, 420, 453, 556
HpySE526I ACGT 1 cut(s) 536
Hsp92II CATG 6 cut(s) 28, 34, 136, 268, 632, 996
HspAI GCGC 1 cut(s) 521
Ksp22I TGATCA 1 cut(s) 214
Kzo9I GATC 4 cut(s) 214, 433, 784, 948
LmnI GCTCC 5 cut(s) 47, 181, 355, 839, 970
Lsp1109I GCAGC 6 cut(s) 493, 580, 750, 852, 976, 985
LweI GCATC 1 cut(s) 916
MaeI CTAG 4 cut(s) 270, 357, 512, 576
MaeII ACGT 1 cut(s) 536
MaeIII GTNAC 2 cut(s) 309, 631
MalI GATC 4 cut(s) 216, 435, 786, 950
MboI GATC 4 cut(s) 214, 433, 784, 948
MboII GAAGA 2 cut(s) 103, 749
MfeI CAATTG 1 cut(s) 569
MflI RGATCY 2 cut(s) 784, 948
MhlI GDGCHC 2 cut(s) 749, 962
MlyI GAGTC 3 cut(s) 62, 451, 830
MmeI TCCRAC 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 707
MseI TTAA 5 cut(s) 35, 390, 675, 792, 900
MslI CAYNNNNRTG 2 cut(s) 23, 29
MspA1I CMGCKG 2 cut(s) 652, 976
MspI CCGG 2 cut(s) 301, 320
MspR9I CCNGG 6 cut(s) 301, 302, 320, 598, 860, 982
MunI CAATTG 1 cut(s) 569
Mva1269I GAATGC 2 cut(s) 224, 338
MvaI CCWGG 3 cut(s) 598, 860, 982
MwoI GCNNNNNNNGC 2 cut(s) 512, 913
NciI CCSGG 3 cut(s) 301, 302, 320
NcoI CCATGG 1 cut(s) 264
NdeII GATC 4 cut(s) 214, 433, 784, 948
NlaIII CATG 6 cut(s) 28, 34, 136, 268, 632, 996
NlaIV GGNNCC 3 cut(s) 158, 193, 602
NmeAIII GCCGAG 1 cut(s) 429
NmuCI GTSAC 1 cut(s) 631
PctI GAATGC 2 cut(s) 224, 338
PdmI GAANNNNTTC 1 cut(s) 707
PfeI GAWTC 4 cut(s) 12, 104, 260, 315
PkrI GCNGC 6 cut(s) 508, 595, 765, 867, 975, 991
PleI GAGTC 3 cut(s) 62, 450, 829
PpsI GAGTC 3 cut(s) 62, 450, 829
PpuMI RGGWCCY 1 cut(s) 231
PshAI GACNNNNGTC 1 cut(s) 605
Psp5II RGGWCCY 1 cut(s) 231
Psp6I CCWGG 3 cut(s) 596, 858, 980
PspFI CCCAGC 1 cut(s) 523
PspGI CCWGG 3 cut(s) 596, 858, 980
PspN4I GGNNCC 3 cut(s) 158, 193, 602
PspPI GGNCC 4 cut(s) 192, 231, 395, 600
PspPPI RGGWCCY 1 cut(s) 231
PsuI RGATCY 2 cut(s) 784, 948
PvuII CAGCTG 2 cut(s) 652, 976
RsaI GTAC 5 cut(s) 146, 293, 473, 554, 875
RsaNI GTAC 5 cut(s) 145, 292, 472, 553, 874
RseI CAYNNNNRTG 2 cut(s) 23, 29
SaqAI TTAA 5 cut(s) 35, 390, 675, 792, 900
SatI GCNGC 6 cut(s) 507, 594, 764, 866, 974, 990
Sau3AI GATC 4 cut(s) 214, 433, 784, 948
Sau96I GGNCC 4 cut(s) 192, 231, 395, 600
ScaI AGTACT 1 cut(s) 875
SchI GAGTC 3 cut(s) 62, 451, 830
ScrFI CCNGG 6 cut(s) 301, 302, 320, 598, 860, 982
SduI GDGCHC 2 cut(s) 749, 962
SfaNI GCATC 1 cut(s) 916
SfcI CTRYAG 1 cut(s) 162
SinI GGWCC 3 cut(s) 192, 231, 600
SmaI CCCGGG 1 cut(s) 302
SmiMI CAYNNNNRTG 2 cut(s) 23, 29
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
SsiI CCGC 2 cut(s) 729, 941
SspI AATATT 1 cut(s) 411
SspMI CTAG 4 cut(s) 270, 357, 512, 576
StyD4I CCNGG 6 cut(s) 299, 300, 318, 596, 858, 980
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 95, 778
TaiI ACGT 1 cut(s) 539
TaqI TCGA 1 cut(s) 696
TatI WGTACW 4 cut(s) 144, 471, 552, 873
TfiI GAWTC 4 cut(s) 12, 104, 260, 315
Tru1I TTAA 5 cut(s) 35, 390, 675, 792, 900
Tru9I TTAA 5 cut(s) 35, 390, 675, 792, 900
TscAI CASTG 3 cut(s) 100, 180, 481
TseFI GTSAC 1 cut(s) 631
TseI GCWGC 6 cut(s) 506, 593, 763, 865, 973, 989
Tsp45I GTSAC 1 cut(s) 631
TspDTI ATGAA 2 cut(s) 213, 682
TspGWI ACGGA 1 cut(s) 455
TspMI CCCGGG 1 cut(s) 300
TspRI CASTG 3 cut(s) 100, 180, 481
VpaK11BI GGWCC 3 cut(s) 192, 231, 600
XapI RAATTY 3 cut(s) 560, 718, 732
XcmI CCANNNNNNNNNTGG 2 cut(s) 92, 806
XmaI CCCGGG 1 cut(s) 300
XmnI GAANNNNTTC 1 cut(s) 707
XspI CTAG 4 cut(s) 270, 357, 512, 576
ZrmI AGTACT 1 cut(s) 875
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.