RLG00000006165

DUF1338

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010892
Physical Location & Seq
Reverse (-)
4871897 .. 4874570
2674 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006165

Sequence Viewer

Length: 1005 bp
ATGATTACCCGATTCTCCACCACCATGACCATGTTAAGAAGCTCCTCTATCTCCTCCCCATCACTTGACTCTCTCATTCGTCGCCAACCCAACAGTGTCAATCCCACCAGGAATCTCATCTCCCTCACCAAATCACGTCTTCCCCTGAACCTCAAATACTCAATCTCCATGAACAATCAAAATCTCCGCACCGTTTGTAGCTCCAAATCAGACAATGGATCCCACGACCCTTCTTCTTCTCAGGGAGGTGAATCATTCTTCAGGGGAGTGTTGGCAAACATGGAGGGTGTGTACTTGAGCAGGAACCCTACAGCAAAGTCAGTGTTGGAGCTTGTTGGGTCTGATGAACAGATTAGCTATGATCATTTTGCATTTAGGACCTTTGGGGTGAATGGTTATGGGATTGATTCCATGGCTAGATTTTTCTTGGATTTCGGGTACACTCCCCGGGAGGAGTTACGATTCCCGGCTAAGAAATTGAGGGCATTGTGGTTTGCTCCTCCTAGTTATACCGTTTCTGATGGTGGTGCTGGTGTTAATGGGCCTTTGCCGAGAATATTTATATCTGAGCTTCTTGTAGATCAGATGAGTCCACAAACTCAGGAGATAATCCGTAAGTACACTGAAAACTCTGGTTCTGGAAATAAGTATGCTGCTCTAGCAAGTGCGCTGGGAACTTTGACGTGGGAAAAGCCGTTGTACTCAGAATTTCAGCAATTGGCTAGGGAAAGTGAATATGCTGCCTGGACCCTTGTCAATGGGTATGCAGTCAACCATGTCACAATCTCTGCTCACCAGCTGAAATCTAAATTGAGAGATATTAAAAGCCTCAATCAATTCATCGAGGAGAATGGGTTCAGGTTGAATTCTGAAGGCGGAATTCTGAAAGGTATGTTACATAATTGTAGCACAACAAACACATGTCTGGAACTATCTTATGATGAGATAATGTATAATTGTGTATGCTCTATTTTTCACTTTGATGCCACTCCAATTTTAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1338

Protein Analysis

335

Amino Acids

37.26

Weight (kDa)

7.65

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGLS PF07063 110 - 290 5.8e-30 2-oxoadipate dioxygenase/decarboxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 187, 876
AclWI GGATC 2 cut(s) 213, 226
AcsI RAATTY 3 cut(s) 707, 865, 879
AcuI CTGAAG 2 cut(s) 244, 891
AfaI GTAC 4 cut(s) 293, 440, 620, 701
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 1 cut(s) 865
AjiI CACGTC 2 cut(s) 137, 684
AjnI CCWGG 2 cut(s) 107, 743
AloI GAACNNNNNNTCC 2 cut(s) 839, 871
AluBI AGCT 6 cut(s) 42, 201, 331, 357, 571, 799
AluI AGCT 6 cut(s) 42, 201, 331, 357, 571, 799
AlwI GGATC 2 cut(s) 213, 226
Ama87I CYCGRG 1 cut(s) 447
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 2 cut(s) 653, 740
ApoI RAATTY 3 cut(s) 707, 865, 879
Asp700I GAANNNNTTC 1 cut(s) 854
AspLEI GCGC 1 cut(s) 670
AspS9I GGNCC 3 cut(s) 378, 542, 747
AsuC2I CCSGG 3 cut(s) 448, 449, 467
AsuHPI GGTGA 4 cut(s) 118, 260, 400, 785
AvaI CYCGRG 1 cut(s) 447
AvaII GGWCC 2 cut(s) 378, 747
BamHI GGATCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 640, 727
BccI CCATC 2 cut(s) 67, 515
BceAI ACGGC 1 cut(s) 679
BciT130I CCWGG 2 cut(s) 109, 745
BclI TGATCA 1 cut(s) 361
BcnI CCSGG 3 cut(s) 448, 449, 467
BfaI CTAG 4 cut(s) 417, 504, 659, 723
BfmI CTRYAG 1 cut(s) 309
BisI GCNGC 2 cut(s) 654, 741
BlsI GCNGC 2 cut(s) 655, 742
Bme1390I CCNGG 5 cut(s) 109, 448, 449, 467, 745
Bme18I GGWCC 2 cut(s) 378, 747
BmeT110I CYCGRG 1 cut(s) 447
BmgBI CACGTC 2 cut(s) 137, 684
BmgT120I GGNCC 3 cut(s) 378, 542, 747
BmiI GGNNCC 3 cut(s) 220, 305, 749
BmrFI CCNGG 5 cut(s) 109, 448, 449, 467, 745
BmsI GCATC 1 cut(s) 973
BoxI GACNNNNGTC 1 cut(s) 752
BpiI GAAGAC 1 cut(s) 131
BpuEI CTTGAG 1 cut(s) 316
BpuMI CCSGG 3 cut(s) 448, 449, 467
BsaJI CCNNGG 3 cut(s) 411, 446, 447
BsaXI ACNNNNNCTCC 6 cut(s) 149, 179, 275, 305, 839, 869
BseBI CCWGG 2 cut(s) 109, 745
BseDI CCNNGG 3 cut(s) 411, 446, 447
BseMII CTCAG 4 cut(s) 254, 558, 614, 717
BseRI GAGGAG 5 cut(s) 34, 43, 467, 489, 860
BseXI GCAGC 2 cut(s) 640, 727
BseYI CCCAGC 1 cut(s) 670
BshFI GGCC 1 cut(s) 544
BsiHKCI CYCGRG 1 cut(s) 447
BsiSI CCGG 2 cut(s) 448, 467
BsnI GGCC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 447
Bsp143I GATC 3 cut(s) 218, 361, 580
Bsp19I CCATGG 1 cut(s) 411
BspACI CCGC 2 cut(s) 187, 876
BspANI GGCC 1 cut(s) 544
BspCNI CTCAG 4 cut(s) 253, 559, 613, 716
BspLI GGNNCC 3 cut(s) 220, 305, 749
BspPI GGATC 2 cut(s) 213, 226
BssECI CCNNGG 3 cut(s) 411, 446, 447
BssMI GATC 3 cut(s) 218, 361, 580
BssT1I CCWWGG 1 cut(s) 411
Bst2UI CCWGG 2 cut(s) 109, 745
Bst4CI ACNGT 3 cut(s) 95, 193, 514
BstDEI CTNAG 5 cut(s) 240, 471, 567, 600, 703
BstDSI CCRYGG 1 cut(s) 411
BstHHI GCGC 1 cut(s) 670
BstKTI GATC 3 cut(s) 221, 364, 583
BstMBI GATC 3 cut(s) 218, 361, 580
BstMWI GCNNNNNNNGC 1 cut(s) 659
BstNI CCWGG 2 cut(s) 109, 745
BstNSI RCATGY 1 cut(s) 924
BstPAI GACNNNNGTC 1 cut(s) 752
BstSCI CCNGG 5 cut(s) 107, 446, 447, 465, 743
BstSFI CTRYAG 1 cut(s) 309
BstV1I GCAGC 2 cut(s) 640, 727
BstV2I GAAGAC 1 cut(s) 131
BstX2I RGATCY 1 cut(s) 218
BstYI RGATCY 1 cut(s) 218
BsuRI GGCC 1 cut(s) 544
BtgI CCRYGG 1 cut(s) 411
BtrI CACGTC 2 cut(s) 137, 684
BtsIMutI CAGTG 3 cut(s) 100, 327, 621
CfoI GCGC 1 cut(s) 670
Cfr13I GGNCC 3 cut(s) 378, 542, 747
Cfr9I CCCGGG 1 cut(s) 447
Csp6I GTAC 4 cut(s) 292, 439, 619, 700
CviAII CATG 7 cut(s) 25, 31, 169, 280, 412, 776, 921
CviQI GTAC 4 cut(s) 292, 439, 619, 700
DdeI CTNAG 5 cut(s) 240, 471, 567, 600, 703
DpnI GATC 3 cut(s) 220, 363, 582
DpnII GATC 3 cut(s) 218, 361, 580
EciI GGCGGA 1 cut(s) 891
Eco130I CCWWGG 1 cut(s) 411
Eco47I GGWCC 2 cut(s) 378, 747
Eco57I CTGAAG 2 cut(s) 244, 891
Eco88I CYCGRG 1 cut(s) 447
EcoO109I RGGNCCY 1 cut(s) 378
EcoRI GAATTC 2 cut(s) 865, 879
EcoRII CCWGG 2 cut(s) 107, 743
EcoT14I CCWWGG 1 cut(s) 411
ErhI CCWWGG 1 cut(s) 411
FaeI CATG 7 cut(s) 28, 34, 172, 283, 415, 779, 924
FatI CATG 7 cut(s) 24, 30, 168, 279, 411, 775, 920
FbaI TGATCA 1 cut(s) 361
Fnu4HI GCNGC 2 cut(s) 654, 741
Fsp4HI GCNGC 2 cut(s) 654, 741
FspBI CTAG 4 cut(s) 417, 504, 659, 723
GlaI GCGC 1 cut(s) 669
GluI GCNGC 2 cut(s) 654, 741
GsaI CCCAGC 1 cut(s) 674
HaeIII GGCC 1 cut(s) 544
HapII CCGG 2 cut(s) 448, 467
HhaI GCGC 1 cut(s) 670
Hin1II CATG 7 cut(s) 28, 34, 172, 283, 415, 779, 924
Hin6I GCGC 1 cut(s) 668
HinP1I GCGC 1 cut(s) 668
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HinfI GANTC 7 cut(s) 12, 68, 112, 251, 407, 462, 589
HpaII CCGG 2 cut(s) 448, 467
HphI GGTGA 4 cut(s) 118, 260, 400, 785
Hpy166II GTNNAC 5 cut(s) 292, 441, 593, 621, 772
Hpy188I TCNGA 8 cut(s) 211, 343, 520, 568, 585, 706, 871, 885
Hpy188III TCNNGA 3 cut(s) 602, 639, 926
Hpy8I GTNNAC 5 cut(s) 292, 441, 593, 621, 772
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 2 cut(s) 240, 866
HpyCH4III ACNGT 3 cut(s) 95, 193, 514
HpyCH4IV ACGT 2 cut(s) 136, 683
HpyCH4V TGCA 2 cut(s) 371, 767
HpyF10VI GCNNNNNNNGC 1 cut(s) 659
HpyF3I CTNAG 5 cut(s) 240, 471, 567, 600, 703
HpySE526I ACGT 2 cut(s) 136, 683
Hsp92II CATG 7 cut(s) 28, 34, 172, 283, 415, 779, 924
HspAI GCGC 1 cut(s) 668
Ksp22I TGATCA 1 cut(s) 361
Kzo9I GATC 3 cut(s) 218, 361, 580
LmnI GCTCC 4 cut(s) 47, 206, 328, 502
Lsp1109I GCAGC 2 cut(s) 640, 727
LweI GCATC 1 cut(s) 973
MaeI CTAG 4 cut(s) 417, 504, 659, 723
MaeII ACGT 2 cut(s) 136, 683
MaeIII GTNAC 3 cut(s) 456, 778, 894
MalI GATC 3 cut(s) 220, 363, 582
MboI GATC 3 cut(s) 218, 361, 580
MboII GAAGA 4 cut(s) 131, 225, 228, 250
MfeI CAATTG 1 cut(s) 716
MflI RGATCY 1 cut(s) 218
MlyI GAGTC 2 cut(s) 62, 598
MmeI TCCRAC 1 cut(s) 306
MroXI GAANNNNTTC 1 cut(s) 854
MseI TTAA 3 cut(s) 35, 537, 822
MslI CAYNNNNRTG 3 cut(s) 23, 29, 981
MspA1I CMGCKG 1 cut(s) 799
MspI CCGG 2 cut(s) 448, 467
MspR9I CCNGG 5 cut(s) 109, 448, 449, 467, 745
MunI CAATTG 1 cut(s) 716
MvaI CCWGG 2 cut(s) 109, 745
MwoI GCNNNNNNNGC 1 cut(s) 659
NciI CCSGG 3 cut(s) 448, 449, 467
NcoI CCATGG 1 cut(s) 411
NdeII GATC 3 cut(s) 218, 361, 580
NlaIII CATG 7 cut(s) 28, 34, 172, 283, 415, 779, 924
NlaIV GGNNCC 3 cut(s) 220, 305, 749
NmeAIII GCCGAG 1 cut(s) 576
NmuCI GTSAC 1 cut(s) 778
NspI RCATGY 1 cut(s) 924
PciI ACATGT 1 cut(s) 920
PdmI GAANNNNTTC 1 cut(s) 854
PfeI GAWTC 5 cut(s) 12, 112, 251, 407, 462
PkrI GCNGC 2 cut(s) 655, 742
PleI GAGTC 2 cut(s) 62, 597
PpsI GAGTC 2 cut(s) 62, 597
PpuMI RGGWCCY 1 cut(s) 378
PscI ACATGT 1 cut(s) 920
PshAI GACNNNNGTC 1 cut(s) 752
Psp5II RGGWCCY 1 cut(s) 378
Psp6I CCWGG 2 cut(s) 107, 743
PspFI CCCAGC 1 cut(s) 670
PspGI CCWGG 2 cut(s) 107, 743
PspN4I GGNNCC 3 cut(s) 220, 305, 749
PspPI GGNCC 3 cut(s) 378, 542, 747
PspPPI RGGWCCY 1 cut(s) 378
PsrI GAACNNNNNNTAC 2 cut(s) 140, 172
PsuI RGATCY 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 799
RsaI GTAC 4 cut(s) 293, 440, 620, 701
RsaNI GTAC 4 cut(s) 292, 439, 619, 700
RseI CAYNNNNRTG 3 cut(s) 23, 29, 981
SaqAI TTAA 3 cut(s) 35, 537, 822
SatI GCNGC 2 cut(s) 654, 741
Sau3AI GATC 3 cut(s) 218, 361, 580
Sau96I GGNCC 3 cut(s) 378, 542, 747
SchI GAGTC 2 cut(s) 62, 598
ScrFI CCNGG 5 cut(s) 109, 448, 449, 467, 745
SfaNI GCATC 1 cut(s) 973
SfcI CTRYAG 1 cut(s) 309
SinI GGWCC 2 cut(s) 378, 747
SmaI CCCGGG 1 cut(s) 449
SmiMI CAYNNNNRTG 3 cut(s) 23, 29, 981
SmlI CTYRAG 1 cut(s) 295
SmoI CTYRAG 1 cut(s) 295
SsiI CCGC 2 cut(s) 187, 876
SspI AATATT 1 cut(s) 558
SspMI CTAG 4 cut(s) 417, 504, 659, 723
StyD4I CCNGG 5 cut(s) 107, 446, 447, 465, 743
StyI CCWWGG 1 cut(s) 411
TaaI ACNGT 3 cut(s) 95, 193, 514
TaiI ACGT 2 cut(s) 139, 686
TaqI TCGA 1 cut(s) 843
TatI WGTACW 3 cut(s) 291, 618, 699
TfiI GAWTC 5 cut(s) 12, 112, 251, 407, 462
Tru1I TTAA 3 cut(s) 35, 537, 822
Tru9I TTAA 3 cut(s) 35, 537, 822
TscAI CASTG 3 cut(s) 100, 327, 628
TseFI GTSAC 1 cut(s) 778
TseI GCWGC 2 cut(s) 653, 740
Tsp45I GTSAC 1 cut(s) 778
TspDTI ATGAA 3 cut(s) 185, 360, 829
TspGWI ACGGA 1 cut(s) 602
TspMI CCCGGG 1 cut(s) 447
TspRI CASTG 3 cut(s) 100, 327, 628
VpaK11BI GGWCC 2 cut(s) 378, 747
XapI RAATTY 3 cut(s) 707, 865, 879
XceI RCATGY 1 cut(s) 924
XmaI CCCGGG 1 cut(s) 447
XmnI GAANNNNTTC 1 cut(s) 854
XspI CTAG 4 cut(s) 417, 504, 659, 723
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.